AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015091


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015091.t1 MTR_3g102980 94.958 238 7 3 1 235 1 236 1.57e-150 418
MS.gene015091.t1 MTR_1g018420 55.102 245 83 8 1 233 1 230 5.89e-78 234
MS.gene015091.t1 MTR_1g106730 48.846 260 86 14 1 230 3 245 4.05e-56 179
MS.gene015091.t1 MTR_4g028610 30.693 202 72 6 30 163 134 335 4.72e-19 85.9
MS.gene015091.t1 MTR_0160s0030 40.152 132 53 4 84 215 240 345 5.99e-19 84.7
MS.gene015091.t1 MTR_4g027440 45.652 92 48 2 76 165 212 303 6.35e-19 84.7
MS.gene015091.t1 MTR_1g067650 30.769 169 90 3 58 209 1 159 2.16e-18 79.7
MS.gene015091.t1 MTR_1g067640 30.357 168 90 3 59 209 1 158 1.48e-17 77.4
MS.gene015091.t1 MTR_7g023560 34.375 128 70 2 52 179 12 125 1.03e-16 75.9
MS.gene015091.t1 MTR_1g090703 30.415 217 96 9 40 203 89 303 1.19e-16 78.2
MS.gene015091.t1 MTR_1g062780 31.683 202 81 7 52 207 81 271 2.36e-16 76.6
MS.gene015091.t1 MTR_5g099040 35.915 142 66 3 48 164 94 235 2.58e-16 76.3
MS.gene015091.t1 MTR_5g062300 39.130 138 73 3 38 164 71 208 3.71e-16 75.5
MS.gene015091.t1 MTR_1g115345 37.984 129 68 3 34 162 2 118 9.56e-16 73.6
MS.gene015091.t1 MTR_1g067660 33.529 170 85 5 47 209 6 154 2.86e-15 71.2
MS.gene015091.t1 MTR_7g100100 43.038 79 38 2 84 162 34 105 5.64e-15 70.5
MS.gene015091.t1 MTR_4g109340 38.514 148 49 8 85 200 349 486 2.68e-14 72.0
MS.gene015091.t1 MTR_2g096540 35.714 126 49 3 37 162 2 95 2.68e-14 67.8
MS.gene015091.t1 MTR_2g084315 29.907 214 96 6 41 200 60 273 7.33e-14 69.7
MS.gene015091.t1 MTR_4g100860 30.435 207 94 8 15 200 124 301 2.21e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015091.t1 AT1G27730 51.899 237 96 7 1 231 1 225 3.56e-62 194
MS.gene015091.t1 AT5G04340 49.576 236 98 10 1 222 1 229 1.53e-55 177
MS.gene015091.t1 AT3G19580 45.802 262 102 10 1 227 1 257 4.78e-50 165
MS.gene015091.t1 AT3G19580 45.802 262 102 10 1 227 1 257 4.78e-50 165
MS.gene015091.t1 AT5G43170 48.718 234 70 11 1 226 1 192 5.39e-46 152
MS.gene015091.t1 AT3G49930 49.143 175 68 5 30 200 36 193 9.84e-40 136
MS.gene015091.t1 AT5G67450 41.250 240 98 8 1 200 1 237 1.76e-39 137
MS.gene015091.t1 AT2G45120 40.237 169 78 5 44 200 143 300 2.88e-24 98.6
MS.gene015091.t1 AT3G60580 37.209 172 79 4 44 200 119 276 8.76e-24 97.1
MS.gene015091.t1 AT2G28200 33.178 214 105 7 44 220 68 280 1.05e-22 94.0
MS.gene015091.t1 AT1G49900 51.579 95 40 2 85 178 194 283 8.58e-22 94.0
MS.gene015091.t1 AT1G49900 40.000 135 72 3 83 217 749 874 6.36e-18 82.8
MS.gene015091.t1 AT5G56200 47.423 97 38 2 84 167 342 438 1.42e-20 90.1
MS.gene015091.t1 AT5G03510 33.166 199 93 5 43 203 64 260 5.29e-19 84.0
MS.gene015091.t1 AT1G02040 31.771 192 94 8 5 163 61 248 1.68e-18 83.2
MS.gene015091.t1 AT2G37430 38.095 126 67 2 37 162 2 116 7.73e-17 75.9
MS.gene015091.t1 AT4G35280 38.400 125 53 4 48 164 138 246 1.20e-16 77.4
MS.gene015091.t1 AT1G02030 34.194 155 79 4 47 200 123 255 2.74e-16 76.3
MS.gene015091.t1 AT2G17180 30.625 160 76 5 48 200 121 252 7.77e-16 75.1
MS.gene015091.t1 AT3G46080 40.171 117 46 3 49 162 12 107 1.13e-15 72.4
MS.gene015091.t1 AT3G46090 36.923 130 62 3 39 167 3 113 1.79e-15 72.0
MS.gene015091.t1 AT5G59820 37.815 119 52 3 55 173 18 114 2.72e-15 71.2
MS.gene015091.t1 AT5G04390 28.889 225 90 7 48 203 96 319 6.23e-15 73.6
MS.gene015091.t1 AT3G53600 34.127 126 71 2 37 162 2 115 8.45e-15 70.5
MS.gene015091.t1 AT2G28710 39.640 111 43 3 52 162 14 100 5.33e-14 67.8
MS.gene015091.t1 AT3G10470 38.235 102 40 4 84 162 182 283 1.73e-12 66.6
MS.gene015091.t1 AT3G46070 34.545 110 55 3 53 162 16 108 2.24e-12 63.5
MS.gene015091.t1 AT5G61470 37.179 78 39 2 86 163 230 297 9.15e-11 60.8

Find 63 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTACTGGACAGGCTTT+GGG 0.284852 3.2:-85377373 MS.gene015091:CDS
TTGCGGACATAACAGCTTTC+CGG 0.302034 3.2:+85377500 None:intergenic
CTTCCCTACTGGACAGGCTT+TGG 0.307779 3.2:-85377374 MS.gene015091:CDS
GTTTCAAGATAACTCAGATT+TGG 0.369889 3.2:+85377757 None:intergenic
TACTCCTTTCACACCCTTTG+AGG 0.385893 3.2:-85377782 MS.gene015091:CDS
GGCAGCGTTGGTGCCGGTGC+CGG 0.399502 3.2:-85377325 MS.gene015091:CDS
CTCAGATTTGGTTCCTCAAA+GGG 0.424837 3.2:+85377769 None:intergenic
GTTGGTGCCGGTGCCGGTGC+TGG 0.425482 3.2:-85377319 MS.gene015091:CDS
CATAACAGCTTTCCGGTGAC+TGG 0.439265 3.2:+85377507 None:intergenic
GGCTTCTTTGCAGCAGGTAA+AGG 0.446061 3.2:+85377169 None:intergenic
AATTTCCAGCTTGAAAAGAC+AGG 0.452679 3.2:+85377147 None:intergenic
CTGATCTTCAGCGGTGGTTG+CGG 0.460016 3.2:+85377483 None:intergenic
TTTGAAAAGTCCGGAAAAGC+CGG 0.466243 3.2:+85377223 None:intergenic
ACTGGCTTTGTGTCCACCTA+GGG 0.475209 3.2:+85377525 None:intergenic
GATCGTTTACCTTTCGTCCA+CGG 0.485045 3.2:+85377733 None:intergenic
CTTACCGTTGGAAGCAGAGC+TGG 0.488264 3.2:+85377432 None:intergenic
GCATTTGTGACTGAGTTTAA+CGG 0.491121 3.2:+85377582 None:intergenic
ATGAGTCTTGTTCTTACCGT+TGG 0.503655 3.2:+85377420 None:intergenic
TGTCACAAATCCTTCCCTAC+TGG 0.506283 3.2:-85377385 MS.gene015091:CDS
AGACAGGGCTTCTTTGCAGC+AGG 0.516606 3.2:+85377163 None:intergenic
GGTTCCTCAAAGGGTGTGAA+AGG 0.517530 3.2:+85377778 None:intergenic
ACGATCAAAGCGTTCTCGCA+TGG 0.518878 3.2:-85377716 MS.gene015091:CDS
TACGAAGGCAGCGTTGGTGC+CGG 0.519961 3.2:-85377331 MS.gene015091:CDS
TTGAAAAGTCCGGAAAAGCC+GGG 0.523321 3.2:+85377224 None:intergenic
GACTGGCTTTGTGTCCACCT+AGG 0.526559 3.2:+85377524 None:intergenic
CAAATCCTTCCCTACTGGAC+AGG 0.527321 3.2:-85377380 MS.gene015091:CDS
CTGAGTTTAACGGTGGTTAG+CGG 0.533899 3.2:+85377592 None:intergenic
CCTCCCAAAGCCTGTCCAGT+AGG 0.538116 3.2:+85377370 None:intergenic
ACTCAGATTTGGTTCCTCAA+AGG 0.539745 3.2:+85377768 None:intergenic
AGGGAAGGATTTGTGACAGA+TGG 0.550231 3.2:+85377390 None:intergenic
TTCTCATCTTATCAAGCCCT+AGG 0.550654 3.2:-85377541 MS.gene015091:CDS
GTAACTGCCTCTGAAGGAGT+TGG 0.551176 3.2:-85377286 MS.gene015091:CDS
CTCCCAAAGCCTGTCCAGTA+GGG 0.562622 3.2:+85377371 None:intergenic
CTTGAAACACCGTGGACGAA+AGG 0.563216 3.2:-85377742 MS.gene015091:CDS
TGTGAAAGGAGTAGCAGTGG+TGG 0.569120 3.2:+85377792 None:intergenic
TGTCACTACGAAGGCAGCGT+TGG 0.571767 3.2:-85377337 MS.gene015091:CDS
GGTAGTCACGGCGGATGAAG+TGG 0.575582 3.2:+85377453 None:intergenic
GCTACTTCCAGCACCGGCAC+CGG 0.578492 3.2:+85377312 None:intergenic
TAAGATCAAAATCACGGTGG+TGG 0.583762 3.2:+85377251 None:intergenic
CAAAGCCTGTCCAGTAGGGA+AGG 0.583815 3.2:+85377375 None:intergenic
GGAAGCAGAGCTGGTAGTCA+CGG 0.585255 3.2:+85377441 None:intergenic
CTCATCATGCTTGCTCGCAG+CGG 0.585296 3.2:-85377652 MS.gene015091:CDS
GTGGACACAAAGCCAGTCAC+CGG 0.585980 3.2:-85377519 MS.gene015091:CDS
ATTTCCAGCTTGAAAAGACA+GGG 0.591447 3.2:+85377148 None:intergenic
TACAGCGCTACTTCCAGCAC+CGG 0.591699 3.2:+85377306 None:intergenic
ATTATTGATCAGTAATGAGA+AGG 0.592327 3.2:+85377124 None:intergenic
GTGAAAGGAGTAGCAGTGGT+GGG 0.592634 3.2:+85377793 None:intergenic
AGCGCTGTAACTGCCTCTGA+AGG 0.607763 3.2:-85377292 MS.gene015091:CDS
CCTACTGGACAGGCTTTGGG+AGG 0.612476 3.2:-85377370 MS.gene015091:CDS
TCATCTTATCAAGCCCTAGG+TGG 0.625860 3.2:-85377538 MS.gene015091:CDS
TGACGATCCAACTCCTTCAG+AGG 0.633382 3.2:+85377279 None:intergenic
GGGTGTGAAAGGAGTAGCAG+TGG 0.640901 3.2:+85377789 None:intergenic
ACTACCAGCTCTGCTTCCAA+CGG 0.645257 3.2:-85377436 MS.gene015091:CDS
GGAGGTTAAGATCAAAATCA+CGG 0.658091 3.2:+85377245 None:intergenic
AGTCACGGCGGATGAAGTGG+TGG 0.674100 3.2:+85377456 None:intergenic
CACAAGCGTTGTCACTACGA+AGG 0.677003 3.2:-85377346 MS.gene015091:CDS
TTTGTGACTGAGTTTAACGG+TGG 0.692042 3.2:+85377585 None:intergenic
AGCAGAGCTGGTAGTCACGG+CGG 0.700777 3.2:+85377444 None:intergenic
GGTGATCTGATCTTCAGCGG+TGG 0.700953 3.2:+85377477 None:intergenic
GGTGGTGATCTGATCTTCAG+CGG 0.704088 3.2:+85377474 None:intergenic
AAAAGTCCGGAAAAGCCGGG+AGG 0.720865 3.2:+85377227 None:intergenic
TGAGTTATCTTGAAACACCG+TGG 0.739154 3.2:-85377750 MS.gene015091:CDS
GGTTAAGATCAAAATCACGG+TGG 0.807668 3.2:+85377248 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACTTCTTTGAAAAGTC+CGG + chr3.2:85377738-85377757 None:intergenic 25.0%
GTTTCAAGATAACTCAGATT+TGG + chr3.2:85377195-85377214 None:intergenic 30.0%
! CTTTTCAAAGAAGTTTTTCG+TGG - chr3.2:85377740-85377759 MS.gene015091:CDS 30.0%
AATTTCCAGCTTGAAAAGAC+AGG + chr3.2:85377805-85377824 None:intergenic 35.0%
ATTTCCAGCTTGAAAAGACA+GGG + chr3.2:85377804-85377823 None:intergenic 35.0%
CAACGACAAAAAGTCTGATT+CGG - chr3.2:85377326-85377345 MS.gene015091:CDS 35.0%
GCATTTGTGACTGAGTTTAA+CGG + chr3.2:85377370-85377389 None:intergenic 35.0%
GGAGGTTAAGATCAAAATCA+CGG + chr3.2:85377707-85377726 None:intergenic 35.0%
!! TGATTTTGATCTTAACCTCC+CGG - chr3.2:85377707-85377726 MS.gene015091:CDS 35.0%
GGTTAAGATCAAAATCACGG+TGG + chr3.2:85377704-85377723 None:intergenic 40.0%
TAAGATCAAAATCACGGTGG+TGG + chr3.2:85377701-85377720 None:intergenic 40.0%
TGAGTTATCTTGAAACACCG+TGG - chr3.2:85377199-85377218 MS.gene015091:CDS 40.0%
TTCTCATCTTATCAAGCCCT+AGG - chr3.2:85377408-85377427 MS.gene015091:CDS 40.0%
TTTGAAAAGTCCGGAAAAGC+CGG + chr3.2:85377729-85377748 None:intergenic 40.0%
TTTGTGACTGAGTTTAACGG+TGG + chr3.2:85377367-85377386 None:intergenic 40.0%
! ACTCAGATTTGGTTCCTCAA+AGG + chr3.2:85377184-85377203 None:intergenic 40.0%
! CTCAGATTTGGTTCCTCAAA+GGG + chr3.2:85377183-85377202 None:intergenic 40.0%
!! ATGAGTCTTGTTCTTACCGT+TGG + chr3.2:85377532-85377551 None:intergenic 40.0%
AGGGAAGGATTTGTGACAGA+TGG + chr3.2:85377562-85377581 None:intergenic 45.0%
CTGAGTTTAACGGTGGTTAG+CGG + chr3.2:85377360-85377379 None:intergenic 45.0%
GATCGTTTACCTTTCGTCCA+CGG + chr3.2:85377219-85377238 None:intergenic 45.0%
TACTCCTTTCACACCCTTTG+AGG - chr3.2:85377167-85377186 MS.gene015091:CDS 45.0%
TCATCTTATCAAGCCCTAGG+TGG - chr3.2:85377411-85377430 MS.gene015091:CDS 45.0%
TGTCACAAATCCTTCCCTAC+TGG - chr3.2:85377564-85377583 MS.gene015091:CDS 45.0%
TTGAAAAGTCCGGAAAAGCC+GGG + chr3.2:85377728-85377747 None:intergenic 45.0%
TTGCGGACATAACAGCTTTC+CGG + chr3.2:85377452-85377471 None:intergenic 45.0%
! GAAGTTTTTCGTGGATGACG+AGG - chr3.2:85377749-85377768 MS.gene015091:CDS 45.0%
!! CGACAAAAAGTCTGATTCGG+TGG - chr3.2:85377329-85377348 MS.gene015091:CDS 45.0%
ACTACCAGCTCTGCTTCCAA+CGG - chr3.2:85377513-85377532 MS.gene015091:CDS 50.0%
CAAATCCTTCCCTACTGGAC+AGG - chr3.2:85377569-85377588 MS.gene015091:CDS 50.0%
CATAACAGCTTTCCGGTGAC+TGG + chr3.2:85377445-85377464 None:intergenic 50.0%
CTTGAAACACCGTGGACGAA+AGG - chr3.2:85377207-85377226 MS.gene015091:CDS 50.0%
GGCTTCTTTGCAGCAGGTAA+AGG + chr3.2:85377783-85377802 None:intergenic 50.0%
GGTGGTGATCTGATCTTCAG+CGG + chr3.2:85377478-85377497 None:intergenic 50.0%
GTGAAAGGAGTAGCAGTGGT+GGG + chr3.2:85377159-85377178 None:intergenic 50.0%
TGACGATCCAACTCCTTCAG+AGG + chr3.2:85377673-85377692 None:intergenic 50.0%
TGTGAAAGGAGTAGCAGTGG+TGG + chr3.2:85377160-85377179 None:intergenic 50.0%
TTCCCTACTGGACAGGCTTT+GGG - chr3.2:85377576-85377595 MS.gene015091:CDS 50.0%
! ACGATCAAAGCGTTCTCGCA+TGG - chr3.2:85377233-85377252 MS.gene015091:CDS 50.0%
! CACAAGCGTTGTCACTACGA+AGG - chr3.2:85377603-85377622 MS.gene015091:CDS 50.0%
! GGTTCCTCAAAGGGTGTGAA+AGG + chr3.2:85377174-85377193 None:intergenic 50.0%
! GTAACTGCCTCTGAAGGAGT+TGG - chr3.2:85377663-85377682 MS.gene015091:CDS 50.0%
! TCTTAACCTCCCGGCTTTTC+CGG - chr3.2:85377716-85377735 MS.gene015091:CDS 50.0%
!! ACTGGCTTTGTGTCCACCTA+GGG + chr3.2:85377427-85377446 None:intergenic 50.0%
!! GAAGCCCTGTCTTTTCAAGC+TGG - chr3.2:85377797-85377816 MS.gene015091:CDS 50.0%
AAAAGTCCGGAAAAGCCGGG+AGG + chr3.2:85377725-85377744 None:intergenic 55.0%
AGACAGGGCTTCTTTGCAGC+AGG + chr3.2:85377789-85377808 None:intergenic 55.0%
AGCGCTGTAACTGCCTCTGA+AGG - chr3.2:85377657-85377676 MS.gene015091:CDS 55.0%
CAAAGCCTGTCCAGTAGGGA+AGG + chr3.2:85377577-85377596 None:intergenic 55.0%
CTCATCATGCTTGCTCGCAG+CGG - chr3.2:85377297-85377316 MS.gene015091:CDS 55.0%
CTCCCAAAGCCTGTCCAGTA+GGG + chr3.2:85377581-85377600 None:intergenic 55.0%
CTGATCTTCAGCGGTGGTTG+CGG + chr3.2:85377469-85377488 None:intergenic 55.0%
CTTACCGTTGGAAGCAGAGC+TGG + chr3.2:85377520-85377539 None:intergenic 55.0%
CTTCCCTACTGGACAGGCTT+TGG - chr3.2:85377575-85377594 MS.gene015091:CDS 55.0%
GGAAGCAGAGCTGGTAGTCA+CGG + chr3.2:85377511-85377530 None:intergenic 55.0%
GGGTGTGAAAGGAGTAGCAG+TGG + chr3.2:85377163-85377182 None:intergenic 55.0%
GGTGATCTGATCTTCAGCGG+TGG + chr3.2:85377475-85377494 None:intergenic 55.0%
GTGGACACAAAGCCAGTCAC+CGG - chr3.2:85377430-85377449 MS.gene015091:CDS 55.0%
TACAGCGCTACTTCCAGCAC+CGG + chr3.2:85377646-85377665 None:intergenic 55.0%
TGTCACTACGAAGGCAGCGT+TGG - chr3.2:85377612-85377631 MS.gene015091:CDS 55.0%
!! GACTGGCTTTGTGTCCACCT+AGG + chr3.2:85377428-85377447 None:intergenic 55.0%
AGCAGAGCTGGTAGTCACGG+CGG + chr3.2:85377508-85377527 None:intergenic 60.0%
AGTCACGGCGGATGAAGTGG+TGG + chr3.2:85377496-85377515 None:intergenic 60.0%
CCTCCCAAAGCCTGTCCAGT+AGG + chr3.2:85377582-85377601 None:intergenic 60.0%
GGTAGTCACGGCGGATGAAG+TGG + chr3.2:85377499-85377518 None:intergenic 60.0%
!! CCTACTGGACAGGCTTTGGG+AGG - chr3.2:85377579-85377598 MS.gene015091:CDS 60.0%
!! TACGAAGGCAGCGTTGGTGC+CGG - chr3.2:85377618-85377637 MS.gene015091:CDS 60.0%
!! GCTACTTCCAGCACCGGCAC+CGG + chr3.2:85377640-85377659 None:intergenic 65.0%
!! GGCAGCGTTGGTGCCGGTGC+CGG - chr3.2:85377624-85377643 MS.gene015091:CDS 75.0%
!! GTTGGTGCCGGTGCCGGTGC+TGG - chr3.2:85377630-85377649 MS.gene015091:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 85377132 85377839 85377132 ID=MS.gene015091
chr3.2 mRNA 85377132 85377839 85377132 ID=MS.gene015091.t1;Parent=MS.gene015091
chr3.2 exon 85377132 85377839 85377132 ID=MS.gene015091.t1.exon1;Parent=MS.gene015091.t1
chr3.2 CDS 85377132 85377839 85377132 ID=cds.MS.gene015091.t1;Parent=MS.gene015091.t1
Gene Sequence

>MS.gene015091

ATGGCTATGGAAGCACTTAACTCACCCACCACTGCTACTCCTTTCACACCCTTTGAGGAACCAAATCTGAGTTATCTTGAAACACCGTGGACGAAAGGTAAACGATCAAAGCGTTCTCGCATGGATCAATCTTCATGCACTGAAGAAGAGTATCTCGCTCTTTGTCTCATCATGCTTGCTCGCAGCGGTAACAACAACGACAAAAAGTCTGATTCGGTGGCGACGCCGCTAACCACCGTTAAACTCAGTCACAAATGCTCAGTCTGCAACAAAGCTTTCTCATCTTATCAAGCCCTAGGTGGACACAAAGCCAGTCACCGGAAAGCTGTTATGTCCGCAACCACCGCTGAAGATCAGATCACCACCACTTCATCCGCCGTGACTACCAGCTCTGCTTCCAACGGTAAGAACAAGACTCATGAGTGTTCCATCTGTCACAAATCCTTCCCTACTGGACAGGCTTTGGGAGGACACAAGCGTTGTCACTACGAAGGCAGCGTTGGTGCCGGTGCCGGTGCTGGAAGTAGCGCTGTAACTGCCTCTGAAGGAGTTGGATCGTCACACAGCCACCACCGTGATTTTGATCTTAACCTCCCGGCTTTTCCGGACTTTTCAAAGAAGTTTTTCGTGGATGACGAGGTTTCTAGTCCTTTACCTGCTGCAAAGAAGCCCTGTCTTTTCAAGCTGGAAATTCCTTCTCATTACTGA

Protein sequence

>MS.gene015091.t1

MAMEALNSPTTATPFTPFEEPNLSYLETPWTKGKRSKRSRMDQSSCTEEEYLALCLIMLARSGNNNDKKSDSVATPLTTVKLSHKCSVCNKAFSSYQALGGHKASHRKAVMSATTAEDQITTTSSAVTTSSASNGKNKTHECSICHKSFPTGQALGGHKRCHYEGSVGAGAGAGSSAVTASEGVGSSHSHHRDFDLNLPAFPDFSKKFFVDDEVSSPLPAAKKPCLFKLEIPSHY