Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38597.t1 | CAB77055.1 | 99.6 | 235 | 1 | 0 | 1 | 235 | 1 | 235 | 1.30E-95 | 359.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38597.t1 | Q96289 | 49.8 | 227 | 101 | 6 | 1 | 224 | 1 | 217 | 1.3e-32 | 141.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38597.t1 | Q9LWA5 | 99.6 | 235 | 1 | 0 | 1 | 235 | 1 | 235 | 9.3e-96 | 359.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene38597.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene38597 | MS.gene044101 | PPI |
| MS.gene001465 | MS.gene38597 | PPI |
| MS.gene015091 | MS.gene38597 | PPI |
| MS.gene38597 | MS.gene00489 | PPI |
| MS.gene38597 | MS.gene38598 | PPI |
| MS.gene38597 | MS.gene012885 | PPI |
| MS.gene38597 | MS.gene015091 | PPI |
| MS.gene38597 | MS.gene001466 | PPI |
| MS.gene38597 | MS.gene66447 | PPI |
| MS.gene38597 | MS.gene24464 | PPI |
| MS.gene38597 | MS.gene32887 | PPI |
| MS.gene38597 | MS.gene001465 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38597.t1 | MTR_3g102980 | 94.958 | 238 | 7 | 3 | 1 | 235 | 1 | 236 | 1.57e-150 | 418 |
| MS.gene38597.t1 | MTR_1g018420 | 55.102 | 245 | 83 | 8 | 1 | 233 | 1 | 230 | 5.89e-78 | 234 |
| MS.gene38597.t1 | MTR_1g106730 | 48.846 | 260 | 86 | 14 | 1 | 230 | 3 | 245 | 4.05e-56 | 179 |
| MS.gene38597.t1 | MTR_4g028610 | 30.693 | 202 | 72 | 6 | 30 | 163 | 134 | 335 | 4.72e-19 | 85.9 |
| MS.gene38597.t1 | MTR_0160s0030 | 40.152 | 132 | 53 | 4 | 84 | 215 | 240 | 345 | 5.99e-19 | 84.7 |
| MS.gene38597.t1 | MTR_4g027440 | 45.652 | 92 | 48 | 2 | 76 | 165 | 212 | 303 | 6.35e-19 | 84.7 |
| MS.gene38597.t1 | MTR_1g067650 | 30.769 | 169 | 90 | 3 | 58 | 209 | 1 | 159 | 2.16e-18 | 79.7 |
| MS.gene38597.t1 | MTR_1g067640 | 30.357 | 168 | 90 | 3 | 59 | 209 | 1 | 158 | 1.48e-17 | 77.4 |
| MS.gene38597.t1 | MTR_7g023560 | 34.375 | 128 | 70 | 2 | 52 | 179 | 12 | 125 | 1.03e-16 | 75.9 |
| MS.gene38597.t1 | MTR_1g090703 | 30.415 | 217 | 96 | 9 | 40 | 203 | 89 | 303 | 1.19e-16 | 78.2 |
| MS.gene38597.t1 | MTR_1g062780 | 31.683 | 202 | 81 | 7 | 52 | 207 | 81 | 271 | 2.36e-16 | 76.6 |
| MS.gene38597.t1 | MTR_5g099040 | 35.915 | 142 | 66 | 3 | 48 | 164 | 94 | 235 | 2.58e-16 | 76.3 |
| MS.gene38597.t1 | MTR_5g062300 | 39.130 | 138 | 73 | 3 | 38 | 164 | 71 | 208 | 3.71e-16 | 75.5 |
| MS.gene38597.t1 | MTR_1g115345 | 37.984 | 129 | 68 | 3 | 34 | 162 | 2 | 118 | 9.56e-16 | 73.6 |
| MS.gene38597.t1 | MTR_1g067660 | 33.529 | 170 | 85 | 5 | 47 | 209 | 6 | 154 | 2.86e-15 | 71.2 |
| MS.gene38597.t1 | MTR_7g100100 | 43.038 | 79 | 38 | 2 | 84 | 162 | 34 | 105 | 5.64e-15 | 70.5 |
| MS.gene38597.t1 | MTR_4g109340 | 38.514 | 148 | 49 | 8 | 85 | 200 | 349 | 486 | 2.68e-14 | 72.0 |
| MS.gene38597.t1 | MTR_2g096540 | 35.714 | 126 | 49 | 3 | 37 | 162 | 2 | 95 | 2.68e-14 | 67.8 |
| MS.gene38597.t1 | MTR_2g084315 | 29.907 | 214 | 96 | 6 | 41 | 200 | 60 | 273 | 7.33e-14 | 69.7 |
| MS.gene38597.t1 | MTR_4g100860 | 30.435 | 207 | 94 | 8 | 15 | 200 | 124 | 301 | 2.21e-13 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38597.t1 | AT1G27730 | 51.899 | 237 | 96 | 7 | 1 | 231 | 1 | 225 | 3.56e-62 | 194 |
| MS.gene38597.t1 | AT5G04340 | 49.576 | 236 | 98 | 10 | 1 | 222 | 1 | 229 | 1.53e-55 | 177 |
| MS.gene38597.t1 | AT3G19580 | 45.802 | 262 | 102 | 10 | 1 | 227 | 1 | 257 | 4.78e-50 | 165 |
| MS.gene38597.t1 | AT3G19580 | 45.802 | 262 | 102 | 10 | 1 | 227 | 1 | 257 | 4.78e-50 | 165 |
| MS.gene38597.t1 | AT5G43170 | 48.718 | 234 | 70 | 11 | 1 | 226 | 1 | 192 | 5.39e-46 | 152 |
| MS.gene38597.t1 | AT3G49930 | 49.143 | 175 | 68 | 5 | 30 | 200 | 36 | 193 | 9.84e-40 | 136 |
| MS.gene38597.t1 | AT5G67450 | 41.250 | 240 | 98 | 8 | 1 | 200 | 1 | 237 | 1.76e-39 | 137 |
| MS.gene38597.t1 | AT2G45120 | 40.237 | 169 | 78 | 5 | 44 | 200 | 143 | 300 | 2.88e-24 | 98.6 |
| MS.gene38597.t1 | AT3G60580 | 37.209 | 172 | 79 | 4 | 44 | 200 | 119 | 276 | 8.76e-24 | 97.1 |
| MS.gene38597.t1 | AT2G28200 | 33.178 | 214 | 105 | 7 | 44 | 220 | 68 | 280 | 1.05e-22 | 94.0 |
| MS.gene38597.t1 | AT1G49900 | 51.579 | 95 | 40 | 2 | 85 | 178 | 194 | 283 | 8.58e-22 | 94.0 |
| MS.gene38597.t1 | AT1G49900 | 40.000 | 135 | 72 | 3 | 83 | 217 | 749 | 874 | 6.36e-18 | 82.8 |
| MS.gene38597.t1 | AT5G56200 | 47.423 | 97 | 38 | 2 | 84 | 167 | 342 | 438 | 1.42e-20 | 90.1 |
| MS.gene38597.t1 | AT5G03510 | 33.166 | 199 | 93 | 5 | 43 | 203 | 64 | 260 | 5.29e-19 | 84.0 |
| MS.gene38597.t1 | AT1G02040 | 31.771 | 192 | 94 | 8 | 5 | 163 | 61 | 248 | 1.68e-18 | 83.2 |
| MS.gene38597.t1 | AT2G37430 | 38.095 | 126 | 67 | 2 | 37 | 162 | 2 | 116 | 7.73e-17 | 75.9 |
| MS.gene38597.t1 | AT4G35280 | 38.400 | 125 | 53 | 4 | 48 | 164 | 138 | 246 | 1.20e-16 | 77.4 |
| MS.gene38597.t1 | AT1G02030 | 34.194 | 155 | 79 | 4 | 47 | 200 | 123 | 255 | 2.74e-16 | 76.3 |
| MS.gene38597.t1 | AT2G17180 | 30.625 | 160 | 76 | 5 | 48 | 200 | 121 | 252 | 7.77e-16 | 75.1 |
| MS.gene38597.t1 | AT3G46080 | 40.171 | 117 | 46 | 3 | 49 | 162 | 12 | 107 | 1.13e-15 | 72.4 |
| MS.gene38597.t1 | AT3G46090 | 36.923 | 130 | 62 | 3 | 39 | 167 | 3 | 113 | 1.79e-15 | 72.0 |
| MS.gene38597.t1 | AT5G59820 | 37.815 | 119 | 52 | 3 | 55 | 173 | 18 | 114 | 2.72e-15 | 71.2 |
| MS.gene38597.t1 | AT5G04390 | 28.889 | 225 | 90 | 7 | 48 | 203 | 96 | 319 | 6.23e-15 | 73.6 |
| MS.gene38597.t1 | AT3G53600 | 34.127 | 126 | 71 | 2 | 37 | 162 | 2 | 115 | 8.45e-15 | 70.5 |
| MS.gene38597.t1 | AT2G28710 | 39.640 | 111 | 43 | 3 | 52 | 162 | 14 | 100 | 5.33e-14 | 67.8 |
| MS.gene38597.t1 | AT3G10470 | 38.235 | 102 | 40 | 4 | 84 | 162 | 182 | 283 | 1.73e-12 | 66.6 |
| MS.gene38597.t1 | AT3G46070 | 34.545 | 110 | 55 | 3 | 53 | 162 | 16 | 108 | 2.24e-12 | 63.5 |
| MS.gene38597.t1 | AT5G61470 | 37.179 | 78 | 39 | 2 | 86 | 163 | 230 | 297 | 9.15e-11 | 60.8 |
Find 0 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACTTCTTTGAAAAGTC+CGG | + | 23841:14939-14958 | MS.gene38597:intergenic | 25.0% |
| GTTTCAAGATAACTCAGATT+TGG | + | 23841:14396-14415 | MS.gene38597:intergenic | 30.0% | |
| ! | CTTTTCAAAGAAGTTTTTCG+TGG | - | 23841:14941-14960 | MS.gene38597:CDS | 30.0% |
| AATTTCCAGCTTGAAAAGAC+AGG | + | 23841:15006-15025 | MS.gene38596:intergenic | 35.0% | |
| ATTTCCAGCTTGAAAAGACA+GGG | + | 23841:15005-15024 | MS.gene38597:intergenic | 35.0% | |
| CAACGACAAAAAGTCTGATT+CGG | - | 23841:14527-14546 | MS.gene38597:CDS | 35.0% | |
| GCATTTGTGACTGAGTTTAA+CGG | + | 23841:14571-14590 | MS.gene38597:intergenic | 35.0% | |
| GGAGGTTAAGATCAAAATCA+CGG | + | 23841:14908-14927 | MS.gene38597:intergenic | 35.0% | |
| !! | TGATTTTGATCTTAACCTCC+CGG | - | 23841:14908-14927 | MS.gene38597:CDS | 35.0% |
| GGTTAAGATCAAAATCACGG+TGG | + | 23841:14905-14924 | MS.gene38597:intergenic | 40.0% | |
| TAAGATCAAAATCACGGTGG+TGG | + | 23841:14902-14921 | MS.gene38597:intergenic | 40.0% | |
| TGAGTTATCTTGAAACACCG+TGG | - | 23841:14400-14419 | MS.gene38597:CDS | 40.0% | |
| TTCTCATCTTATCAAGCCCT+AGG | - | 23841:14609-14628 | MS.gene38597:CDS | 40.0% | |
| TTTGAAAAGTCCGGAAAAGC+CGG | + | 23841:14930-14949 | MS.gene38597:intergenic | 40.0% | |
| TTTGTGACTGAGTTTAACGG+TGG | + | 23841:14568-14587 | MS.gene38597:intergenic | 40.0% | |
| ! | ACTCAGATTTGGTTCCTCAA+AGG | + | 23841:14385-14404 | MS.gene38597:intergenic | 40.0% |
| ! | CTCAGATTTGGTTCCTCAAA+GGG | + | 23841:14384-14403 | MS.gene38597:intergenic | 40.0% |
| !! | ATGAGTCTTGTTCTTACCGT+TGG | + | 23841:14733-14752 | MS.gene38597:intergenic | 40.0% |
| AGGGAAGGATTTGTGACAGA+TGG | + | 23841:14763-14782 | MS.gene38597:intergenic | 45.0% | |
| CTGAGTTTAACGGTGGTTAG+CGG | + | 23841:14561-14580 | MS.gene38597:intergenic | 45.0% | |
| GATCGTTTACCTTTCGTCCA+CGG | + | 23841:14420-14439 | MS.gene38597:intergenic | 45.0% | |
| TACTCCTTTCACACCCTTTG+AGG | - | 23841:14368-14387 | MS.gene38597:CDS | 45.0% | |
| TCATCTTATCAAGCCCTAGG+TGG | - | 23841:14612-14631 | MS.gene38597:CDS | 45.0% | |
| TGTCACAAATCCTTCCCTAC+TGG | - | 23841:14765-14784 | MS.gene38597:CDS | 45.0% | |
| TTGAAAAGTCCGGAAAAGCC+GGG | + | 23841:14929-14948 | MS.gene38597:intergenic | 45.0% | |
| TTGCGGACATAACAGCTTTC+CGG | + | 23841:14653-14672 | MS.gene38597:intergenic | 45.0% | |
| ! | GAAGTTTTTCGTGGATGACG+AGG | - | 23841:14950-14969 | MS.gene38597:CDS | 45.0% |
| !! | CGACAAAAAGTCTGATTCGG+TGG | - | 23841:14530-14549 | MS.gene38597:CDS | 45.0% |
| ACTACCAGCTCTGCTTCCAA+CGG | - | 23841:14714-14733 | MS.gene38597:CDS | 50.0% | |
| CAAATCCTTCCCTACTGGAC+AGG | - | 23841:14770-14789 | MS.gene38597:CDS | 50.0% | |
| CATAACAGCTTTCCGGTGAC+TGG | + | 23841:14646-14665 | MS.gene38597:intergenic | 50.0% | |
| CTTGAAACACCGTGGACGAA+AGG | - | 23841:14408-14427 | MS.gene38597:CDS | 50.0% | |
| GGCTTCTTTGCAGCAGGTAA+AGG | + | 23841:14984-15003 | MS.gene38597:intergenic | 50.0% | |
| GGTGGTGATCTGATCTTCAG+CGG | + | 23841:14679-14698 | MS.gene38597:intergenic | 50.0% | |
| GTGAAAGGAGTAGCAGTGGT+GGG | + | 23841:14360-14379 | MS.gene38597:intergenic | 50.0% | |
| TGACGATCCAACTCCTTCAG+AGG | + | 23841:14874-14893 | MS.gene38597:intergenic | 50.0% | |
| TGTGAAAGGAGTAGCAGTGG+TGG | + | 23841:14361-14380 | MS.gene38597:intergenic | 50.0% | |
| TTCCCTACTGGACAGGCTTT+GGG | - | 23841:14777-14796 | MS.gene38597:CDS | 50.0% | |
| ! | ACGATCAAAGCGTTCTCGCA+TGG | - | 23841:14434-14453 | MS.gene38597:CDS | 50.0% |
| ! | CACAAGCGTTGTCACTACGA+AGG | - | 23841:14804-14823 | MS.gene38597:CDS | 50.0% |
| ! | GGTTCCTCAAAGGGTGTGAA+AGG | + | 23841:14375-14394 | MS.gene38597:intergenic | 50.0% |
| ! | GTAACTGCCTCTGAAGGAGT+TGG | - | 23841:14864-14883 | MS.gene38597:CDS | 50.0% |
| ! | TCTTAACCTCCCGGCTTTTC+CGG | - | 23841:14917-14936 | MS.gene38597:CDS | 50.0% |
| !! | ACTGGCTTTGTGTCCACCTA+GGG | + | 23841:14628-14647 | MS.gene38597:intergenic | 50.0% |
| !! | GAAGCCCTGTCTTTTCAAGC+TGG | - | 23841:14998-15017 | MS.gene38597:CDS | 50.0% |
| AAAAGTCCGGAAAAGCCGGG+AGG | + | 23841:14926-14945 | MS.gene38596:intergenic | 55.0% | |
| AGACAGGGCTTCTTTGCAGC+AGG | + | 23841:14990-15009 | MS.gene38597:intergenic | 55.0% | |
| AGCGCTGTAACTGCCTCTGA+AGG | - | 23841:14858-14877 | MS.gene38597:CDS | 55.0% | |
| CAAAGCCTGTCCAGTAGGGA+AGG | + | 23841:14778-14797 | MS.gene38597:intergenic | 55.0% | |
| CTCATCATGCTTGCTCGCAG+CGG | - | 23841:14498-14517 | MS.gene38597:CDS | 55.0% | |
| CTCCCAAAGCCTGTCCAGTA+GGG | + | 23841:14782-14801 | MS.gene38597:intergenic | 55.0% | |
| CTGATCTTCAGCGGTGGTTG+CGG | + | 23841:14670-14689 | MS.gene38597:intergenic | 55.0% | |
| CTTACCGTTGGAAGCAGAGC+TGG | + | 23841:14721-14740 | MS.gene38597:intergenic | 55.0% | |
| CTTCCCTACTGGACAGGCTT+TGG | - | 23841:14776-14795 | MS.gene38597:CDS | 55.0% | |
| GGAAGCAGAGCTGGTAGTCA+CGG | + | 23841:14712-14731 | MS.gene38597:intergenic | 55.0% | |
| GGGTGTGAAAGGAGTAGCAG+TGG | + | 23841:14364-14383 | MS.gene38597:intergenic | 55.0% | |
| GGTGATCTGATCTTCAGCGG+TGG | + | 23841:14676-14695 | MS.gene38597:intergenic | 55.0% | |
| GTGGACACAAAGCCAGTCAC+CGG | - | 23841:14631-14650 | MS.gene38597:CDS | 55.0% | |
| TACAGCGCTACTTCCAGCAC+CGG | + | 23841:14847-14866 | MS.gene38597:intergenic | 55.0% | |
| TGTCACTACGAAGGCAGCGT+TGG | - | 23841:14813-14832 | MS.gene38597:CDS | 55.0% | |
| !! | GACTGGCTTTGTGTCCACCT+AGG | + | 23841:14629-14648 | MS.gene38597:intergenic | 55.0% |
| AGCAGAGCTGGTAGTCACGG+CGG | + | 23841:14709-14728 | MS.gene38597:intergenic | 60.0% | |
| AGTCACGGCGGATGAAGTGG+TGG | + | 23841:14697-14716 | MS.gene38597:intergenic | 60.0% | |
| CCTCCCAAAGCCTGTCCAGT+AGG | + | 23841:14783-14802 | MS.gene38597:intergenic | 60.0% | |
| GGTAGTCACGGCGGATGAAG+TGG | + | 23841:14700-14719 | MS.gene38597:intergenic | 60.0% | |
| !! | CCTACTGGACAGGCTTTGGG+AGG | - | 23841:14780-14799 | MS.gene38597:CDS | 60.0% |
| !! | TACGAAGGCAGCGTTGGTGC+CGG | - | 23841:14819-14838 | MS.gene38597:CDS | 60.0% |
| !! | GCTACTTCCAGCACCGGCAC+CGG | + | 23841:14841-14860 | MS.gene38597:intergenic | 65.0% |
| !! | GGCAGCGTTGGTGCCGGTGC+CGG | - | 23841:14825-14844 | MS.gene38597:CDS | 75.0% |
| !! | GTTGGTGCCGGTGCCGGTGC+TGG | - | 23841:14831-14850 | MS.gene38597:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23841 | gene | 14333 | 15040 | 14333 | ID=MS.gene38597 |
| 23841 | mRNA | 14333 | 15040 | 14333 | ID=MS.gene38597.t1;Parent=MS.gene38597 |
| 23841 | exon | 14333 | 15040 | 14333 | ID=MS.gene38597.t1.exon1;Parent=MS.gene38597.t1 |
| 23841 | CDS | 14333 | 15040 | 14333 | ID=cds.MS.gene38597.t1;Parent=MS.gene38597.t1 |
| Gene Sequence |
| Protein sequence |
