AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004034

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004034.t1 MTR_4g063975 98.450 258 4 0 1 258 1 258 0.0 525
MS.gene004034.t1 MTR_2g097030 87.209 258 33 0 1 258 3 260 7.05e-175 482
MS.gene004034.t1 MTR_5g079950 82.661 248 43 0 11 258 12 259 2.63e-161 447
MS.gene004034.t1 MTR_7g061720 82.171 258 45 1 2 258 1 258 5.96e-161 447
MS.gene004034.t1 MTR_5g041700 66.800 250 81 1 8 257 1 248 1.62e-124 354
MS.gene004034.t1 MTR_4g081950 65.217 253 81 3 2 253 34 280 1.34e-122 350
MS.gene004034.t1 MTR_3g094060 64.314 255 86 3 5 256 1 253 5.56e-122 348
MS.gene004034.t1 MTR_3g094040 64.173 254 86 3 5 256 3 253 7.22e-122 347
MS.gene004034.t1 MTR_5g075320 63.780 254 87 3 5 256 1 251 4.22e-120 343
MS.gene004034.t1 MTR_3g435430 70.222 225 65 1 32 256 25 247 9.52e-120 342
MS.gene004034.t1 MTR_3g466760 63.281 256 92 1 1 256 7 260 3.80e-117 336
MS.gene004034.t1 MTR_3g466790 63.424 257 90 3 1 256 1 254 2.03e-116 334
MS.gene004034.t1 MTR_7g011060 63.265 245 80 2 12 256 6 240 9.97e-115 329
MS.gene004034.t1 MTR_7g111010 57.312 253 100 4 2 251 1 248 7.82e-107 310
MS.gene004034.t1 MTR_1g085460 61.157 242 86 4 16 254 18 254 1.30e-106 309
MS.gene004034.t1 MTR_6g012520 62.698 252 84 2 5 256 1 242 3.74e-105 305
MS.gene004034.t1 MTR_1g026020 57.422 256 103 3 1 254 1 252 7.24e-105 305
MS.gene004034.t1 MTR_7g110990 62.780 223 79 3 32 251 12 233 2.22e-103 300
MS.gene004034.t1 MTR_4g124340 59.471 227 89 2 30 255 59 283 7.91e-103 300
MS.gene004034.t1 MTR_8g464350 55.061 247 106 2 5 251 7 248 1.70e-100 293
MS.gene004034.t1 MTR_7g013150 55.738 244 105 2 11 254 10 250 5.15e-98 287
MS.gene004034.t1 MTR_8g058990 57.207 222 93 1 32 253 30 249 7.77e-95 279
MS.gene004034.t1 MTR_7g013140 57.399 223 93 1 32 254 28 248 4.86e-94 277
MS.gene004034.t1 MTR_7g013120 52.653 245 111 3 11 254 10 250 1.36e-93 276
MS.gene004034.t1 MTR_5g011210 50.394 254 123 3 6 257 11 263 1.59e-92 273
MS.gene004034.t1 MTR_4g102450 50.394 254 123 3 6 257 10 262 1.16e-91 271
MS.gene004034.t1 MTR_0040s0140 47.287 258 131 2 1 256 2 256 1.17e-88 263
MS.gene004034.t1 MTR_5g076950 55.310 226 100 1 31 256 40 264 7.48e-88 262
MS.gene004034.t1 MTR_2g090255 46.341 246 131 1 12 256 7 252 5.53e-83 249
MS.gene004034.t1 MTR_3g064510 52.340 235 103 5 31 257 52 285 1.41e-79 241
MS.gene004034.t1 MTR_0008s0120 59.091 110 45 0 144 253 86 195 3.96e-42 143
MS.gene004034.t1 MTR_0008s0120 56.471 85 33 2 5 88 7 88 2.92e-24 96.7
MS.gene004034.t1 MTR_6g012500 42.857 175 84 4 98 256 106 280 1.93e-40 141
MS.gene004034.t1 MTR_2g090270 30.041 243 144 9 24 254 27 255 5.82e-25 100
MS.gene004034.t1 MTR_2g090265 28.947 266 160 11 4 254 2 253 1.93e-23 95.9
MS.gene004034.t1 MTR_0122s0020 31.780 236 135 11 31 254 40 261 9.38e-23 94.4
MS.gene004034.t1 MTR_4g028640 29.237 236 132 11 32 251 42 258 4.14e-18 81.6
MS.gene004034.t1 MTR_0118s0070 28.571 231 135 12 37 251 53 269 1.86e-16 77.4
MS.gene004034.t1 MTR_0809s0010 28.512 242 137 13 27 251 42 264 2.42e-15 73.9
MS.gene004034.t1 MTR_0122s0020 33.838 198 97 12 21 198 21 204 7.79e-15 71.6
MS.gene004034.t1 MTR_5g013440 25.991 227 151 9 35 254 32 248 1.84e-13 68.6
MS.gene004034.t1 MTR_4g099370 25.751 233 152 10 28 251 24 244 3.81e-13 67.8
MS.gene004034.t1 MTR_1g085365 53.571 56 26 0 201 256 4 59 4.19e-13 63.2
MS.gene004034.t1 MTR_4g075790 28.378 222 124 9 43 248 32 234 4.79e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004034.t1 AT2G39700 82.879 257 44 0 2 258 1 257 3.97e-164 454
MS.gene004034.t1 AT2G28950 82.490 257 45 0 2 258 1 257 4.14e-156 434
MS.gene004034.t1 AT2G37640 79.447 253 52 0 6 258 10 262 3.81e-154 429
MS.gene004034.t1 AT3G55500 78.346 254 55 0 5 258 7 260 5.09e-154 429
MS.gene004034.t1 AT5G02260 74.419 258 66 0 1 258 1 258 8.36e-152 424
MS.gene004034.t1 AT2G37640 82.381 210 37 0 49 258 28 237 1.97e-132 374
MS.gene004034.t1 AT1G69530 66.798 253 81 2 5 257 1 250 6.81e-125 355
MS.gene004034.t1 AT1G69530 66.798 253 81 2 5 257 1 250 6.81e-125 355
MS.gene004034.t1 AT1G69530 66.403 253 82 2 5 257 1 250 2.60e-123 352
MS.gene004034.t1 AT2G03090 66.935 248 79 2 11 257 8 253 4.62e-122 348
MS.gene004034.t1 AT5G56320 64.844 256 83 3 5 257 1 252 1.07e-120 345
MS.gene004034.t1 AT5G56320 64.844 256 83 3 5 257 1 252 1.07e-120 345
MS.gene004034.t1 AT5G56320 64.844 256 83 3 5 257 1 252 6.84e-120 344
MS.gene004034.t1 AT1G26770 66.403 253 81 2 5 257 1 249 9.40e-118 337
MS.gene004034.t1 AT1G26770 66.142 254 82 2 4 257 10 259 1.29e-117 337
MS.gene004034.t1 AT5G05290 62.109 256 93 3 2 256 3 255 4.97e-115 330
MS.gene004034.t1 AT2G40610 62.698 252 88 3 6 256 7 253 3.22e-114 328
MS.gene004034.t1 AT3G29030 65.929 226 68 2 32 257 38 254 3.93e-110 318
MS.gene004034.t1 AT1G69530 61.660 253 64 3 5 257 1 220 4.74e-109 314
MS.gene004034.t1 AT1G69530 61.265 253 65 3 5 257 1 220 1.26e-107 311
MS.gene004034.t1 AT4G01630 61.066 244 87 5 11 251 11 249 5.74e-106 307
MS.gene004034.t1 AT1G20190 61.538 221 82 2 34 252 28 247 1.56e-97 286
MS.gene004034.t1 AT5G39290 50.000 278 111 5 1 254 3 276 1.30e-95 282
MS.gene004034.t1 AT5G39270 50.000 278 111 4 1 254 3 276 4.23e-95 281
MS.gene004034.t1 AT1G62980 52.800 250 116 2 6 254 5 253 9.64e-93 274
MS.gene004034.t1 AT5G39280 55.157 223 96 2 32 254 54 272 1.31e-89 266
MS.gene004034.t1 AT1G12560 51.807 249 116 4 11 257 16 262 1.86e-89 266
MS.gene004034.t1 AT5G39300 47.636 275 119 5 1 254 3 273 7.35e-89 265
MS.gene004034.t1 AT5G39310 54.911 224 96 3 32 254 90 309 2.30e-87 262
MS.gene004034.t1 AT3G03220 53.509 228 105 1 31 258 39 265 3.89e-86 257
MS.gene004034.t1 AT4G38210 45.565 248 133 1 12 257 8 255 1.14e-80 243
MS.gene004034.t1 AT5G39260 47.191 267 120 6 1 255 3 260 1.81e-76 233
MS.gene004034.t1 AT1G20190 57.979 188 76 2 67 252 2 188 2.90e-76 229
MS.gene004034.t1 AT3G15370 46.512 258 123 7 1 254 1 247 9.12e-76 231
MS.gene004034.t1 AT3G15370 41.667 288 123 8 1 254 1 277 3.30e-69 215
MS.gene004034.t1 AT4G28250 30.705 241 138 11 28 254 32 257 9.76e-24 97.1
MS.gene004034.t1 AT2G20750 29.114 237 144 9 31 254 39 264 7.88e-22 92.0
MS.gene004034.t1 AT1G65680 30.126 239 138 11 28 251 43 267 4.08e-20 87.4
MS.gene004034.t1 AT4G28250 29.583 240 136 11 28 254 32 251 5.68e-20 86.7
MS.gene004034.t1 AT2G20750 32.386 176 97 7 31 195 39 203 3.50e-16 75.5
MS.gene004034.t1 AT4G28250 32.240 183 95 8 28 200 32 195 1.16e-15 73.9
MS.gene004034.t1 AT4G28250 32.584 178 91 8 28 195 32 190 7.20e-15 71.6
MS.gene004034.t1 AT3G60570 23.774 265 172 9 9 258 10 259 3.02e-13 68.2
MS.gene004034.t1 AT4G17030 25.000 212 138 7 3 209 4 199 5.62e-13 67.4
MS.gene004034.t1 AT3G45970 25.000 212 137 9 51 251 15 215 5.68e-12 64.3

Find 67 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCAATGTAGAGCATTT+TGG 0.215132 4.3:-39707287 None:intergenic
TCACTTGGTTGAGCAAAATT+AGG 0.253485 4.3:-39707874 None:intergenic
GCTCCTCCTAGTTGGCAATT+TGG 0.267981 4.3:+39709562 MS.gene004034:CDS
GACAAAGAGTGGTGTCATTC+TGG 0.278401 4.3:+39707811 MS.gene004034:CDS
GATGCTCATGCTACTTTCTA+TGG 0.312345 4.3:+39707379 MS.gene004034:CDS
TTTGCTCAACCAAGTGATAA+TGG 0.336925 4.3:+39707880 MS.gene004034:CDS
TATGGTGGATCTGATGCTTC+TGG 0.339901 4.3:+39707397 MS.gene004034:CDS
GCCAGTTCTGACCCCAGTTT+CGG 0.341334 4.3:-39709456 None:intergenic
GGATGCCACTGAGCCGAAAC+TGG 0.348922 4.3:+39709443 MS.gene004034:CDS
ATTGTTGAAGCTAGAATTCC+TGG 0.372131 4.3:+39707331 MS.gene004034:CDS
CAATTTGGTCAAACTTTCAC+TGG 0.389947 4.3:+39709577 MS.gene004034:CDS
ACTAACCTGCGGTAAGCAAC+AGG 0.390868 4.3:-39707988 None:intergenic
CAGTGGCATCCACTCGGTTC+GGG 0.402414 4.3:-39709431 None:intergenic
TCAGTGGCATCCACTCGGTT+CGG 0.423453 4.3:-39709432 None:intergenic
ACCGTAGATCTTCAACATCT+TGG 0.445386 4.3:+39709533 MS.gene004034:CDS
GCCGAAACTGGGGTCAGAAC+TGG 0.448238 4.3:+39709455 MS.gene004034:CDS
CACCAACCACCATTATCACT+TGG 0.453463 4.3:-39707889 None:intergenic
CGGATTCCGTTACTTTAACC+TGG 0.456362 4.3:+39709351 MS.gene004034:CDS
GTTTGACCAAATTGCCAACT+AGG 0.460335 4.3:-39709568 None:intergenic
AGAATTCCTGGTGTTTACTC+TGG 0.487043 4.3:+39707343 MS.gene004034:CDS
GGAACATTGCTCCTCCTAGT+TGG 0.491170 4.3:+39709554 MS.gene004034:CDS
GTAGCAGTAATGAAAATTGA+AGG 0.495390 4.3:-39707838 None:intergenic
TCTGATGCTTCTGGCACCAT+GGG 0.503667 4.3:+39707406 MS.gene004034:CDS
TTAGGAGGAGCATGTGGGTA+CGG 0.517583 4.3:+39707682 MS.gene004034:intron
GTGTTTACTCTGGTGGTCAA+TGG 0.519210 4.3:+39707353 MS.gene004034:CDS
ATTCCTGGTGTTTACTCTGG+TGG 0.519949 4.3:+39707346 MS.gene004034:CDS
TTGTCCCTGTTGCTTACCGC+AGG 0.522475 4.3:+39707983 MS.gene004034:CDS
TCAGCAATCTTAAGAAACAT+AGG 0.524078 4.3:-39707946 None:intergenic
AATTTGTACAGTCAAGGTTA+CGG 0.526954 4.3:+39707706 MS.gene004034:CDS
ATTAGCAACGTAGCAGGTGC+TGG 0.528629 4.3:+39709379 MS.gene004034:CDS
ATCTGATGCTTCTGGCACCA+TGG 0.529188 4.3:+39707405 MS.gene004034:CDS
GTGCTAATTAGCAACGTAGC+AGG 0.532373 4.3:+39709373 MS.gene004034:CDS
CTACGTTGCTAATTAGCACC+AGG 0.535801 4.3:-39709369 None:intergenic
AAAGTAACGGAATCCGTTCA+CGG 0.539938 4.3:-39709344 None:intergenic
TCCAAGATGTTGAAGATCTA+CGG 0.544252 4.3:-39709534 None:intergenic
GATGCCACTGAGCCGAAACT+GGG 0.544292 4.3:+39709444 MS.gene004034:CDS
GGAATAAGATTCACCGTGAA+CGG 0.547347 4.3:+39709331 MS.gene004034:CDS
ATCGTGCGCGCCTACGTGAA+AGG 0.554876 4.3:+39709406 MS.gene004034:CDS
TACGGAAATTTGTACAGTCA+AGG 0.556701 4.3:+39707700 MS.gene004034:CDS
ATTCCACCACGCTTTCTGCA+TGG 0.558295 4.3:-39709313 None:intergenic
AAACACGAAAGGACAAAGCC+TGG 0.564825 4.3:-39709501 None:intergenic
AAGATTGCTGAGTATCGTGC+TGG 0.570660 4.3:+39707958 MS.gene004034:CDS
AGCACTGCTTTGTTCAACAA+TGG 0.578791 4.3:+39707748 MS.gene004034:CDS
GGTTCGGGTTCCTTTCACGT+AGG 0.581009 4.3:-39709416 None:intergenic
TCGGCTCAGTGGCATCCACT+CGG 0.585195 4.3:-39709437 None:intergenic
TTCAACAATGGTTTAAGCTG+TGG 0.599006 4.3:+39707760 MS.gene004034:CDS
CTTGGTTGAGCAAAATTAGG+AGG 0.603163 4.3:-39707871 None:intergenic
TTAGCACCAGGTTAAAGTAA+CGG 0.608652 4.3:-39709357 None:intergenic
GGCATAGCAAGATCAAAATG+AGG 0.611060 4.3:-39707925 None:intergenic
TGACCACCAGAGTAAACACC+AGG 0.616969 4.3:-39707349 None:intergenic
GCTCATGCTACTTTCTATGG+TGG 0.618802 4.3:+39707382 MS.gene004034:CDS
TGACCCCAGTTTCGGCTCAG+TGG 0.625730 4.3:-39709448 None:intergenic
CTGGCCCACTAAAACCGCGT+TGG 0.630033 4.3:-39709482 None:intergenic
GCAAGATCAAAATGAGGACG+AGG 0.630560 4.3:-39707919 None:intergenic
GTCACTGCTTGAAACACGAA+AGG 0.633040 4.3:-39709512 None:intergenic
TGACCAAATTGCCAACTAGG+AGG 0.638092 4.3:-39709565 None:intergenic
AGGGTGCCATGCAGAAAGCG+TGG 0.647035 4.3:+39709307 MS.gene004034:intron
AACCAAGTGATAATGGTGGT+TGG 0.649043 4.3:+39707887 MS.gene004034:CDS
AGATCAAAATGAGGACGAGG+AGG 0.649689 4.3:-39707916 None:intergenic
TGAAAGGAACCCGAACCGAG+TGG 0.653524 4.3:+39709422 MS.gene004034:CDS
GATCAAAATGAGGACGAGGA+GGG 0.663864 4.3:-39707915 None:intergenic
GTGCCATGCAGAAAGCGTGG+TGG 0.676430 4.3:+39709310 MS.gene004034:CDS
ATGCCACTGAGCCGAAACTG+GGG 0.678002 4.3:+39709445 MS.gene004034:CDS
AGTGTGCAAATGACAAAGAG+TGG 0.680419 4.3:+39707800 MS.gene004034:CDS
CTAACCTGCGGTAAGCAACA+GGG 0.690792 4.3:-39707987 None:intergenic
GCTCAACCAAGTGATAATGG+TGG 0.719451 4.3:+39707883 MS.gene004034:CDS
TCAGAACTGGCAATCCAACG+CGG 0.772854 4.3:+39709468 MS.gene004034:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTATACTAAAATAAATTA+AGG - chr4.3:39708479-39708498 None:intergenic 10.0%
!!! AAGTTTATTTTATTTTACTT+TGG + chr4.3:39708223-39708242 MS.gene004034:intron 10.0%
!!! ATTTTATCTTTAAGTTATTT+AGG + chr4.3:39708195-39708214 MS.gene004034:intron 10.0%
!!! TTAATTATCCAATTTTTATA+TGG - chr4.3:39709135-39709154 None:intergenic 10.0%
!! CTATCTATATTAAATAGAAA+TGG - chr4.3:39709100-39709119 None:intergenic 15.0%
!! TGAAAAGAAAAAAAAATTAG+AGG + chr4.3:39708404-39708423 MS.gene004034:intron 15.0%
!!! ATATATTATTGACTTTTCTT+TGG + chr4.3:39708531-39708550 MS.gene004034:intron 15.0%
!!! ATGTTTTTTTAGGTTTAATT+AGG - chr4.3:39707620-39707639 None:intergenic 15.0%
!!! TAAAAATAATATAAAGTACC+AGG + chr4.3:39708441-39708460 MS.gene004034:intron 15.0%
!!! TTTATGTTAATTTTTTGTGT+TGG + chr4.3:39707654-39707673 MS.gene004034:intron 15.0%
!!! TTTTCTTTTTCATTAATTGA+GGG - chr4.3:39708504-39708523 None:intergenic 15.0%
!! AATATTTAGGTCTCTTAATA+AGG + chr4.3:39708273-39708292 MS.gene004034:intron 20.0%
!! ATAGTTAAGAAATTAGTTGT+GGG + chr4.3:39708566-39708585 MS.gene004034:intron 20.0%
!! GTTAATGAAAAAAGAAAAGT+AGG + chr4.3:39708609-39708628 MS.gene004034:intron 20.0%
!! TACATTACTTGAACAAAAAA+AGG - chr4.3:39708020-39708039 None:intergenic 20.0%
!! TAGCATGTGTAAAAATAAAA+AGG + chr4.3:39709218-39709237 MS.gene004034:intron 20.0%
!! TATAGTTAAGAAATTAGTTG+TGG + chr4.3:39708565-39708584 MS.gene004034:intron 20.0%
!! TTAAGGTTAAATTATATTCC+TGG - chr4.3:39708462-39708481 None:intergenic 20.0%
!!! ATTTTGTTGATATTTACACA+GGG + chr4.3:39709288-39709307 MS.gene004034:intron 20.0%
!!! CTTTTCTTTTTCATTAATTG+AGG - chr4.3:39708505-39708524 None:intergenic 20.0%
!!! GTAACATTTCATGTTTTTTT+AGG - chr4.3:39707630-39707649 None:intergenic 20.0%
!!! TATTTTGTTGATATTTACAC+AGG + chr4.3:39709287-39709306 MS.gene004034:intron 20.0%
!!! TTTTTGTTCAAGTAATGTAT+GGG + chr4.3:39708021-39708040 MS.gene004034:intron 20.0%
!!! TTTTTTGTTCAAGTAATGTA+TGG + chr4.3:39708020-39708039 MS.gene004034:intron 20.0%
! AAAATGAAGTAGTAAAAGAG+TGG - chr4.3:39708119-39708138 None:intergenic 25.0%
! AAATTGGAATCAATTATGGT+AGG + chr4.3:39709188-39709207 MS.gene004034:intron 25.0%
! AATCACTTTGACATATAGTT+AGG + chr4.3:39707495-39707514 MS.gene004034:intron 25.0%
! AATTGGAATCAATTATGGTA+GGG + chr4.3:39709189-39709208 MS.gene004034:intron 25.0%
! AGAAATGAATGAAGTAACTA+GGG - chr4.3:39708648-39708667 None:intergenic 25.0%
! ATCACTTTGACATATAGTTA+GGG + chr4.3:39707496-39707515 MS.gene004034:intron 25.0%
! ATGGAAAGAATGAAAAAGAA+AGG - chr4.3:39708941-39708960 None:intergenic 25.0%
! CTACTCAACCATATAAAAAT+TGG + chr4.3:39709124-39709143 MS.gene004034:intron 25.0%
! GATACAAGTGGTAAATATTT+AGG + chr4.3:39708260-39708279 MS.gene004034:intron 25.0%
! GTAGAAATTGGAATCAATTA+TGG + chr4.3:39709184-39709203 MS.gene004034:intron 25.0%
! TAAAATATGAGACACTAACA+TGG - chr4.3:39708960-39708979 None:intergenic 25.0%
! TATCCACATGTTTGTATTTA+CGG - chr4.3:39707571-39707590 None:intergenic 25.0%
!! AGTGTCTCATATTTTACTTT+TGG + chr4.3:39708964-39708983 MS.gene004034:intron 25.0%
!! TTTCACTGGAAAGAATTTTA+GGG + chr4.3:39709591-39709610 MS.gene004034:CDS 25.0%
!! TTTTGTTCAAGTAATGTATG+GGG + chr4.3:39708022-39708041 MS.gene004034:intron 25.0%
!!! TTTTTTGTGTTGGTGTTTTT+AGG + chr4.3:39707664-39707683 MS.gene004034:intron 25.0%
AATTTGTACAGTCAAGGTTA+CGG + chr4.3:39707706-39707725 MS.gene004034:CDS 30.0%
AGAAAATATGAGGGCAAAAA+GGG - chr4.3:39707453-39707472 None:intergenic 30.0%
ATAGACGCATTATTATATGC+AGG - chr4.3:39708071-39708090 None:intergenic 30.0%
CAGAAATGAATGAAGTAACT+AGG - chr4.3:39708649-39708668 None:intergenic 30.0%
GACATAATCTCGATGTATTA+GGG - chr4.3:39707541-39707560 None:intergenic 30.0%
GTAGCAGTAATGAAAATTGA+AGG - chr4.3:39707841-39707860 None:intergenic 30.0%
TAGACGCATTATTATATGCA+GGG - chr4.3:39708070-39708089 None:intergenic 30.0%
TCAGCAATCTTAAGAAACAT+AGG - chr4.3:39707949-39707968 None:intergenic 30.0%
TCTTAAGTTTGTGATACAAG+TGG + chr4.3:39708248-39708267 MS.gene004034:intron 30.0%
TGACATAATCTCGATGTATT+AGG - chr4.3:39707542-39707561 None:intergenic 30.0%
! CAATTTTTGTACGAAGAACA+AGG - chr4.3:39708843-39708862 None:intergenic 30.0%
! CTTTCACTGGAAAGAATTTT+AGG + chr4.3:39709590-39709609 MS.gene004034:CDS 30.0%
!!! CAATTTCTACTTTTTCCGTA+CGG - chr4.3:39709174-39709193 None:intergenic 30.0%
AAAGGAAAGTAACTAACCTG+CGG - chr4.3:39708002-39708021 None:intergenic 35.0%
AAATTTAGGTACGTTGACGT+AGG - chr4.3:39709033-39709052 None:intergenic 35.0%
ATTATTATATGCAGGGCTTG+GGG - chr4.3:39708063-39708082 None:intergenic 35.0%
ATTGTTGAAGCTAGAATTCC+TGG + chr4.3:39707331-39707350 MS.gene004034:CDS 35.0%
CAATTTGGTCAAACTTTCAC+TGG + chr4.3:39709577-39709596 MS.gene004034:CDS 35.0%
CAGAAAATATGAGGGCAAAA+AGG - chr4.3:39707454-39707473 None:intergenic 35.0%
CATTATTATATGCAGGGCTT+GGG - chr4.3:39708064-39708083 None:intergenic 35.0%
CCTCAATCCAGAAAATATGA+GGG - chr4.3:39707462-39707481 None:intergenic 35.0%
CGTACGGAAAAAGTAGAAAT+TGG + chr4.3:39709172-39709191 MS.gene004034:intron 35.0%
GTGAAGTGATATATACCGTA+CGG + chr4.3:39709156-39709175 MS.gene004034:intron 35.0%
GTTAAGAAATTAGTTGTGGG+AGG + chr4.3:39708569-39708588 MS.gene004034:intron 35.0%
TACGGAAATTTGTACAGTCA+AGG + chr4.3:39707700-39707719 MS.gene004034:CDS 35.0%
TCACCGTAAATACAAACATG+TGG + chr4.3:39707565-39707584 MS.gene004034:intron 35.0%
TCACTTGGTTGAGCAAAATT+AGG - chr4.3:39707877-39707896 None:intergenic 35.0%
TCCAAGATGTTGAAGATCTA+CGG - chr4.3:39709537-39709556 None:intergenic 35.0%
TTAGCACCAGGTTAAAGTAA+CGG - chr4.3:39709360-39709379 None:intergenic 35.0%
TTCAACAATGGTTTAAGCTG+TGG + chr4.3:39707760-39707779 MS.gene004034:CDS 35.0%
TTCATTAACTGTGATGAGAC+TGG - chr4.3:39708598-39708617 None:intergenic 35.0%
TTCCAACTGAATTAAGCTCT+TGG + chr4.3:39708090-39708109 MS.gene004034:intron 35.0%
TTCCAAGAGCTTAATTCAGT+TGG - chr4.3:39708095-39708114 None:intergenic 35.0%
TTTGCTCAACCAAGTGATAA+TGG + chr4.3:39707880-39707899 MS.gene004034:CDS 35.0%
! CCTCATATTTTCTGGATTGA+GGG + chr4.3:39707460-39707479 MS.gene004034:intron 35.0%
! CTTTTTGCCCTCATATTTTC+TGG + chr4.3:39707452-39707471 MS.gene004034:intron 35.0%
!! GAAAGCAATGTAGAGCATTT+TGG - chr4.3:39707290-39707309 None:intergenic 35.0%
!! GGTAATTTGTTACATACCCA+TGG - chr4.3:39707425-39707444 None:intergenic 35.0%
!! TGATGGTGTTTTCGACTTTT+TGG + chr4.3:39708692-39708711 MS.gene004034:intron 35.0%
!!! TTTGTGTTGGTGTTTTTAGG+AGG + chr4.3:39707667-39707686 MS.gene004034:intron 35.0%
AAGTAATGTATGGGGTTTCC+GGG + chr4.3:39708030-39708049 MS.gene004034:intron 40.0%
ACCGTAGATCTTCAACATCT+TGG + chr4.3:39709533-39709552 MS.gene004034:CDS 40.0%
AGAATTCCTGGTGTTTACTC+TGG + chr4.3:39707343-39707362 MS.gene004034:CDS 40.0%
AGTGTGCAAATGACAAAGAG+TGG + chr4.3:39707800-39707819 MS.gene004034:CDS 40.0%
ATGAGGGCAAAAAGGGAAAT+GGG - chr4.3:39707446-39707465 None:intergenic 40.0%
CAAGTAATGTATGGGGTTTC+CGG + chr4.3:39708029-39708048 MS.gene004034:intron 40.0%
CCCTCAATCCAGAAAATATG+AGG - chr4.3:39707463-39707482 None:intergenic 40.0%
CTTGGTTGAGCAAAATTAGG+AGG - chr4.3:39707874-39707893 None:intergenic 40.0%
GCATTATTATATGCAGGGCT+TGG - chr4.3:39708065-39708084 None:intergenic 40.0%
GGAATAAGATTCACCGTGAA+CGG + chr4.3:39709331-39709350 MS.gene004034:CDS 40.0%
GGCATAGCAAGATCAAAATG+AGG - chr4.3:39707928-39707947 None:intergenic 40.0%
GTGTGTCTGATTCATGAAGT+GGG + chr4.3:39708747-39708766 MS.gene004034:intron 40.0%
GTTGCTCTAGTCAAATGTGA+TGG + chr4.3:39708675-39708694 MS.gene004034:intron 40.0%
GTTTGACCAAATTGCCAACT+AGG - chr4.3:39709571-39709590 None:intergenic 40.0%
TATGAGGGCAAAAAGGGAAA+TGG - chr4.3:39707447-39707466 None:intergenic 40.0%
TGTGTCTGATTCATGAAGTG+GGG + chr4.3:39708748-39708767 MS.gene004034:intron 40.0%
TGTGTGTCTGATTCATGAAG+TGG + chr4.3:39708746-39708765 MS.gene004034:intron 40.0%
! AAAGTAACGGAATCCGTTCA+CGG - chr4.3:39709347-39709366 None:intergenic 40.0%
! AGCACTGCTTTGTTCAACAA+TGG + chr4.3:39707748-39707767 MS.gene004034:CDS 40.0%
! CCCTCATATTTTCTGGATTG+AGG + chr4.3:39707459-39707478 MS.gene004034:intron 40.0%
!! AACCAAGTGATAATGGTGGT+TGG + chr4.3:39707887-39707906 MS.gene004034:CDS 40.0%
!! GATGCTCATGCTACTTTCTA+TGG + chr4.3:39707379-39707398 MS.gene004034:CDS 40.0%
!!! TGTTTTTAGGAGGAGCATGT+GGG + chr4.3:39707677-39707696 MS.gene004034:intron 40.0%
AAACACGAAAGGACAAAGCC+TGG - chr4.3:39709504-39709523 None:intergenic 45.0%
AGATCAAAATGAGGACGAGG+AGG - chr4.3:39707919-39707938 None:intergenic 45.0%
AGTAATGTATGGGGTTTCCG+GGG + chr4.3:39708031-39708050 MS.gene004034:intron 45.0%
ATTCCTGGTGTTTACTCTGG+TGG + chr4.3:39707346-39707365 MS.gene004034:CDS 45.0%
CACCAACCACCATTATCACT+TGG - chr4.3:39707892-39707911 None:intergenic 45.0%
CTACGTTGCTAATTAGCACC+AGG - chr4.3:39709372-39709391 None:intergenic 45.0%
GATCAAAATGAGGACGAGGA+GGG - chr4.3:39707918-39707937 None:intergenic 45.0%
GCAAGATCAAAATGAGGACG+AGG - chr4.3:39707922-39707941 None:intergenic 45.0%
GTCACTGCTTGAAACACGAA+AGG - chr4.3:39709515-39709534 None:intergenic 45.0%
GTGCTAATTAGCAACGTAGC+AGG + chr4.3:39709373-39709392 MS.gene004034:CDS 45.0%
GTGTTTACTCTGGTGGTCAA+TGG + chr4.3:39707353-39707372 MS.gene004034:CDS 45.0%
TGACCAAATTGCCAACTAGG+AGG - chr4.3:39709568-39709587 None:intergenic 45.0%
! AAGATTGCTGAGTATCGTGC+TGG + chr4.3:39707958-39707977 MS.gene004034:CDS 45.0%
! CGGATTCCGTTACTTTAACC+TGG + chr4.3:39709351-39709370 MS.gene004034:CDS 45.0%
!! AAGTGGGGTAGTGTTACTTC+GGG + chr4.3:39708763-39708782 MS.gene004034:intron 45.0%
!! CAATCCAACGCGGTTTTAGT+GGG + chr4.3:39709478-39709497 MS.gene004034:CDS 45.0%
!! GAAGTGGGGTAGTGTTACTT+CGG + chr4.3:39708762-39708781 MS.gene004034:intron 45.0%
!! GACAAAGAGTGGTGTCATTC+TGG + chr4.3:39707811-39707830 MS.gene004034:CDS 45.0%
!! GCTCAACCAAGTGATAATGG+TGG + chr4.3:39707883-39707902 MS.gene004034:CDS 45.0%
!! GCTCATGCTACTTTCTATGG+TGG + chr4.3:39707382-39707401 MS.gene004034:CDS 45.0%
!! GGTAGTGTTACTTCGGGTAA+AGG + chr4.3:39708769-39708788 MS.gene004034:intron 45.0%
!! TATGGTGGATCTGATGCTTC+TGG + chr4.3:39707397-39707416 MS.gene004034:CDS 45.0%
!!! GTGTTTTTAGGAGGAGCATG+TGG + chr4.3:39707676-39707695 MS.gene004034:intron 45.0%
!! TTAAAAAAAAATGAAAATTT+AGG - chr4.3:39709047-39709066 None:intergenic 5.0%
!!! ATTATTTTTAGATTTTATTT+TGG - chr4.3:39708431-39708450 None:intergenic 5.0%
!!! TCATTTTTTTATTATTTTTT+TGG + chr4.3:39708132-39708151 MS.gene004034:intron 5.0%
ACTAACCTGCGGTAAGCAAC+AGG - chr4.3:39707991-39708010 None:intergenic 50.0%
ATCTGATGCTTCTGGCACCA+TGG + chr4.3:39707405-39707424 MS.gene004034:CDS 50.0%
ATTAGCAACGTAGCAGGTGC+TGG + chr4.3:39709379-39709398 MS.gene004034:CDS 50.0%
ATTCCACCACGCTTTCTGCA+TGG - chr4.3:39709316-39709335 None:intergenic 50.0%
CTAACCTGCGGTAAGCAACA+GGG - chr4.3:39707990-39708009 None:intergenic 50.0%
GCTCCTCCTAGTTGGCAATT+TGG + chr4.3:39709562-39709581 MS.gene004034:CDS 50.0%
GGAACATTGCTCCTCCTAGT+TGG + chr4.3:39709554-39709573 MS.gene004034:CDS 50.0%
TCAGAACTGGCAATCCAACG+CGG + chr4.3:39709468-39709487 MS.gene004034:CDS 50.0%
TCTGATGCTTCTGGCACCAT+GGG + chr4.3:39707406-39707425 MS.gene004034:CDS 50.0%
TGACCACCAGAGTAAACACC+AGG - chr4.3:39707352-39707371 None:intergenic 50.0%
! TTAGGAGGAGCATGTGGGTA+CGG + chr4.3:39707682-39707701 MS.gene004034:intron 50.0%
!! GCAATCCAACGCGGTTTTAG+TGG + chr4.3:39709477-39709496 MS.gene004034:CDS 50.0%
ATGCCACTGAGCCGAAACTG+GGG + chr4.3:39709445-39709464 MS.gene004034:CDS 55.0%
GATGCCACTGAGCCGAAACT+GGG + chr4.3:39709444-39709463 MS.gene004034:CDS 55.0%
TCAGTGGCATCCACTCGGTT+CGG - chr4.3:39709435-39709454 None:intergenic 55.0%
TGAAAGGAACCCGAACCGAG+TGG + chr4.3:39709422-39709441 MS.gene004034:CDS 55.0%
! GCCAGTTCTGACCCCAGTTT+CGG - chr4.3:39709459-39709478 None:intergenic 55.0%
! GGTTCGGGTTCCTTTCACGT+AGG - chr4.3:39709419-39709438 None:intergenic 55.0%
! TTGTCCCTGTTGCTTACCGC+AGG + chr4.3:39707983-39708002 MS.gene004034:CDS 55.0%
ATCGTGCGCGCCTACGTGAA+AGG + chr4.3:39709406-39709425 MS.gene004034:CDS 60.0%
CAGTGGCATCCACTCGGTTC+GGG - chr4.3:39709434-39709453 None:intergenic 60.0%
CTGGCCCACTAAAACCGCGT+TGG - chr4.3:39709485-39709504 None:intergenic 60.0%
GCCGAAACTGGGGTCAGAAC+TGG + chr4.3:39709455-39709474 MS.gene004034:CDS 60.0%
GGATGCCACTGAGCCGAAAC+TGG + chr4.3:39709443-39709462 MS.gene004034:CDS 60.0%
TCGGCTCAGTGGCATCCACT+CGG - chr4.3:39709440-39709459 None:intergenic 60.0%
TGACCCCAGTTTCGGCTCAG+TGG - chr4.3:39709451-39709470 None:intergenic 60.0%
! AGGGTGCCATGCAGAAAGCG+TGG + chr4.3:39709307-39709326 MS.gene004034:intron 60.0%
! GTGCCATGCAGAAAGCGTGG+TGG + chr4.3:39709310-39709329 MS.gene004034:CDS 60.0%
!! CAACGCGGTTTTAGTGGGCC+AGG + chr4.3:39709483-39709502 MS.gene004034:CDS 60.0%
AGGGCTTGGGGTTCGAGCCC+CGG - chr4.3:39708051-39708070 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 39707280 39709618 39707280 ID=MS.gene004034
chr4.3 mRNA 39707280 39709618 39707280 ID=MS.gene004034.t1;Parent=MS.gene004034
chr4.3 exon 39707280 39707427 39707280 ID=MS.gene004034.t1.exon1;Parent=MS.gene004034.t1
chr4.3 CDS 39707280 39707427 39707280 ID=cds.MS.gene004034.t1;Parent=MS.gene004034.t1
chr4.3 exon 39707686 39708004 39707686 ID=MS.gene004034.t1.exon2;Parent=MS.gene004034.t1
chr4.3 CDS 39707686 39708004 39707686 ID=cds.MS.gene004034.t1;Parent=MS.gene004034.t1
chr4.3 exon 39709309 39709618 39709309 ID=MS.gene004034.t1.exon3;Parent=MS.gene004034.t1
chr4.3 CDS 39709309 39709618 39709309 ID=cds.MS.gene004034.t1;Parent=MS.gene004034.t1
Gene Sequence

>MS.gene004034

ATGGTGGCCAAAATGCTCTACATTGCTTTCATAATTATCATTTCAATGTACATTGTTGAAGCTAGAATTCCTGGTGTTTACTCTGGTGGTCAATGGCAAGATGCTCATGCTACTTTCTATGGTGGATCTGATGCTTCTGGCACCATGGGTATGTAACAAATTACCCATTTCCCTTTTTGCCCTCATATTTTCTGGATTGAGGGTGAGTTTGACAAAATCACTTTGACATATAGTTAGGGTTACATGTATAGCTTAAAACCCTAATACATCGAGATTATGTCAAATTCACCGTAAATACAAACATGTGGATAAAATACATACAAATAATTACAATGTACCTAATTAAACCTAAAAAAACATGAAATGTTACTATGTTTATGTTAATTTTTTGTGTTGGTGTTTTTAGGAGGAGCATGTGGGTACGGAAATTTGTACAGTCAAGGTTACGGAGTGAACACAGCAGCATTAAGCACTGCTTTGTTCAACAATGGTTTAAGCTGTGGTGCTTGTTTTGAGATAAAGTGTGCAAATGACAAAGAGTGGTGTCATTCTGGAAGTCCTTCAATTTTCATTACTGCTACTAACTTTTGTCCTCCTAATTTTGCTCAACCAAGTGATAATGGTGGTTGGTGTAACCCTCCTCGTCCTCATTTTGATCTTGCTATGCCTATGTTTCTTAAGATTGCTGAGTATCGTGCTGGTATTGTCCCTGTTGCTTACCGCAGGTTAGTTACTTTCCTTTTTTTGTTCAAGTAATGTATGGGGTTTCCGGGGCTCGAACCCCAAGCCCTGCATATAATAATGCGTCTATTCCAACTGAATTAAGCTCTTGGAAACCACTCTTTTACTACTTCATTTTTTTATTATTTTTTTGGTATAAACTTCATTTTTAATTTTAGACGAATTAAACTTTACATTTTATCTTTAAGTTATTTAGGTCTCTAAGTTTATTTTATTTTACTTTGGTGTCTTAAGTTTGTGATACAAGTGGTAAATATTTAGGTCTCTTAATAAGGAAAAACATTTTGTTTCAATGTTTAAATAAAGTCTATAATGCTGTTAGAACAAAAACAATTTATAGAGACAAAGTGTATGATACTTGTCTGTCACTTAAATAACTAAAATGAAAAGAAAAAAAAATTAGAGGACCAAAATAAAATCTAAAAATAATATAAAGTACCAGGAATATAATTTAACCTTAATTTATTTTAGTATAGTACTCCCTCAATTAATGAAAAAGAAAAGTAGTTTATATATTATTGACTTTTCTTTGGAAAATGATGTTTATAGTTAAGAAATTAGTTGTGGGAGGAGTCCAGTCTCATCACAGTTAATGAAAAAAGAAAAGTAGGCTTATATTATTGACCCTAGTTACTTCATTCATTTCTGTATATAGTTGCTCTAGTCAAATGTGATGGTGTTTTCGACTTTTTGGCTATGTAAATTTCTGCACTTCTCACATGATCTGTGTGTCTGATTCATGAAGTGGGGTAGTGTTACTTCGGGTAAAGGATTCTCTTACACACGTAGTATCTTTTTTCTACTGATCACTCACTCTTTCCTTGTTCTTCGTACAAAAATTGTTCGTACAAATATAGCATTGAGCTGTTTAGTTTAGACGAAATTAAGTTATATACTTGAAAAAAATTATATATGTTCCTTTCTTTTTCATTCTTTCCATGTTAGTGTCTCATATTTTACTTTTGGAATCATTTTTCTTTTACTTTTTTAGTCACTTTCAGTGAAATTACCTACGTCAACGTACCTAAATTTTCATTTTTTTTTAATTTATTTATTTTTCTTAACTATATATATGTCCATTTCTATTTAATATAGATAGATATCTACTCAACCATATAAAAATTGGATAATTAAAGTGAAGTGATATATACCGTACGGAAAAAGTAGAAATTGGAATCAATTATGGTAGGGTATAGCTAGCATGTGTAAAAATAAAAAGGATAAGTGAAAAAGTTAATAAAATGTGAAAAGTCAAAAATAGTGACGTATTTTGTTGATATTTACACAGGGTGCCATGCAGAAAGCGTGGTGGAATAAGATTCACCGTGAACGGATTCCGTTACTTTAACCTGGTGCTAATTAGCAACGTAGCAGGTGCTGGTGATATCGTGCGCGCCTACGTGAAAGGAACCCGAACCGAGTGGATGCCACTGAGCCGAAACTGGGGTCAGAACTGGCAATCCAACGCGGTTTTAGTGGGCCAGGCTTTGTCCTTTCGTGTTTCAAGCAGTGACCGTAGATCTTCAACATCTTGGAACATTGCTCCTCCTAGTTGGCAATTTGGTCAAACTTTCACTGGAAAGAATTTTAGGGTTTAA
Protein sequence

>MS.gene004034.t1

MVAKMLYIAFIIIISMYIVEARIPGVYSGGQWQDAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGACFEIKCANDKEWCHSGSPSIFITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKIAEYRAGIVPVAYRRVPCRKRGGIRFTVNGFRYFNLVLISNVAGAGDIVRAYVKGTRTEWMPLSRNWGQNWQSNAVLVGQALSFRVSSSDRRSSTSWNIAPPSWQFGQTFTGKNFRV