AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20357

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20357.t1 MTR_5g011210 96.958 263 8 0 1 263 1 263 0.0 531
MS.gene20357.t1 MTR_4g102450 79.087 263 54 1 1 263 1 262 3.31e-157 437
MS.gene20357.t1 MTR_5g079950 55.128 234 102 3 31 263 27 258 1.14e-92 274
MS.gene20357.t1 MTR_2g097030 53.680 231 105 2 30 259 27 256 2.35e-91 271
MS.gene20357.t1 MTR_5g041700 58.108 222 89 3 37 257 25 243 3.21e-90 267
MS.gene20357.t1 MTR_7g061720 50.781 256 122 4 10 263 4 257 1.13e-89 266
MS.gene20357.t1 MTR_1g085460 54.348 230 99 4 37 262 30 257 1.79e-87 261
MS.gene20357.t1 MTR_3g094060 56.054 223 96 2 37 259 31 251 6.47e-87 259
MS.gene20357.t1 MTR_7g111010 51.550 258 111 6 9 262 7 254 6.83e-87 259
MS.gene20357.t1 MTR_4g081950 55.405 222 95 3 37 257 61 279 1.93e-86 259
MS.gene20357.t1 MTR_7g110990 55.217 230 97 4 37 262 12 239 2.58e-86 257
MS.gene20357.t1 MTR_3g094040 56.109 221 95 2 37 257 31 249 4.22e-86 257
MS.gene20357.t1 MTR_4g063975 50.000 250 123 2 11 259 6 254 4.66e-86 257
MS.gene20357.t1 MTR_3g435430 53.648 233 103 4 27 257 14 243 1.24e-85 256
MS.gene20357.t1 MTR_8g464350 50.000 258 122 5 6 262 3 254 1.33e-85 256
MS.gene20357.t1 MTR_5g075320 51.037 241 116 2 16 256 8 246 3.90e-85 254
MS.gene20357.t1 MTR_1g026020 46.792 265 121 5 1 262 8 255 2.44e-84 253
MS.gene20357.t1 MTR_8g058990 53.913 230 100 4 35 262 28 253 3.41e-83 249
MS.gene20357.t1 MTR_7g013140 54.825 228 99 3 36 262 27 251 5.57e-83 249
MS.gene20357.t1 MTR_7g013120 52.193 228 104 4 37 262 29 253 7.51e-81 244
MS.gene20357.t1 MTR_7g013150 48.638 257 124 4 7 262 4 253 1.28e-80 243
MS.gene20357.t1 MTR_7g011060 52.444 225 97 3 35 259 24 238 3.50e-80 241
MS.gene20357.t1 MTR_3g466760 51.639 244 116 2 13 256 14 255 4.10e-80 242
MS.gene20357.t1 MTR_3g466790 51.230 244 117 2 13 256 8 249 3.36e-79 239
MS.gene20357.t1 MTR_4g124340 49.333 225 109 4 37 259 61 282 5.15e-78 238
MS.gene20357.t1 MTR_6g012520 49.231 260 108 5 1 259 4 240 2.22e-70 217
MS.gene20357.t1 MTR_0040s0140 41.935 248 139 3 16 259 8 254 3.65e-68 211
MS.gene20357.t1 MTR_5g076950 45.778 225 121 1 35 259 39 262 2.37e-67 210
MS.gene20357.t1 MTR_2g090255 39.506 243 145 2 18 259 9 250 7.17e-64 200
MS.gene20357.t1 MTR_3g064510 43.777 233 122 5 35 259 51 282 1.40e-60 193
MS.gene20357.t1 MTR_0008s0120 36.576 257 103 4 6 262 3 199 2.12e-50 164
MS.gene20357.t1 MTR_2g090270 31.868 273 150 11 3 262 9 258 8.51e-32 118
MS.gene20357.t1 MTR_0122s0020 33.333 240 132 10 37 263 41 265 1.86e-30 114
MS.gene20357.t1 MTR_2g090265 31.321 265 153 11 11 262 8 256 1.23e-29 112
MS.gene20357.t1 MTR_4g028640 30.877 285 155 12 1 263 1 265 1.42e-27 107
MS.gene20357.t1 MTR_6g012500 48.485 99 50 1 161 259 181 278 4.32e-25 100
MS.gene20357.t1 MTR_0118s0070 29.958 237 136 9 42 262 53 275 1.31e-21 91.7
MS.gene20357.t1 MTR_0809s0010 28.685 251 145 9 28 262 38 270 7.63e-20 86.7
MS.gene20357.t1 MTR_5g013440 25.490 255 157 7 27 263 13 252 5.06e-19 84.0
MS.gene20357.t1 MTR_0122s0020 32.584 178 94 8 37 202 41 204 1.08e-18 82.0
MS.gene20357.t1 MTR_4g075790 28.634 227 131 6 50 262 34 243 1.33e-15 74.7
MS.gene20357.t1 MTR_4g099370 25.652 230 156 5 40 263 31 251 6.70e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20357.t1 AT1G12560 68.826 247 74 2 17 263 19 262 2.67e-126 359
MS.gene20357.t1 AT1G62980 63.077 260 89 1 4 263 5 257 1.50e-122 350
MS.gene20357.t1 AT2G39700 54.582 251 108 3 11 256 1 250 1.93e-95 281
MS.gene20357.t1 AT3G55500 51.807 249 117 3 10 256 6 253 1.01e-92 274
MS.gene20357.t1 AT2G37640 51.181 254 121 3 11 263 10 261 1.63e-90 268
MS.gene20357.t1 AT2G03090 56.951 223 94 2 37 259 30 250 1.08e-88 264
MS.gene20357.t1 AT1G69530 52.227 247 116 2 11 257 1 245 2.30e-88 263
MS.gene20357.t1 AT1G69530 52.227 247 116 2 11 257 1 245 2.30e-88 263
MS.gene20357.t1 AT1G69530 52.227 247 116 2 11 257 1 245 4.73e-88 263
MS.gene20357.t1 AT5G02260 50.996 251 118 3 10 259 8 254 7.82e-88 261
MS.gene20357.t1 AT2G28950 53.043 230 106 2 31 259 25 253 4.68e-87 259
MS.gene20357.t1 AT1G20190 53.138 239 107 3 27 262 16 252 6.06e-86 256
MS.gene20357.t1 AT4G01630 53.478 230 101 4 37 262 28 255 4.82e-85 254
MS.gene20357.t1 AT5G56320 50.400 250 116 4 11 259 7 249 1.99e-84 253
MS.gene20357.t1 AT5G56320 50.400 250 116 4 11 259 7 249 1.99e-84 253
MS.gene20357.t1 AT5G56320 50.400 250 116 4 11 259 7 249 5.75e-84 253
MS.gene20357.t1 AT2G40610 54.955 222 95 4 37 256 30 248 2.78e-83 250
MS.gene20357.t1 AT2G37640 54.976 211 92 3 54 263 28 236 6.64e-82 246
MS.gene20357.t1 AT5G05290 51.351 222 105 3 37 257 32 251 6.23e-80 241
MS.gene20357.t1 AT1G69530 50.855 234 110 3 11 244 1 229 5.71e-79 239
MS.gene20357.t1 AT1G69530 54.286 210 95 1 11 220 1 209 1.46e-78 237
MS.gene20357.t1 AT1G26770 55.204 221 97 2 37 257 26 244 2.51e-78 237
MS.gene20357.t1 AT1G26770 55.204 221 97 2 37 257 36 254 2.81e-78 237
MS.gene20357.t1 AT5G39270 49.378 241 111 5 28 262 44 279 4.46e-78 238
MS.gene20357.t1 AT5G39300 45.556 270 126 6 9 262 12 276 4.57e-78 238
MS.gene20357.t1 AT5G39280 46.442 267 130 6 1 262 17 275 7.39e-78 237
MS.gene20357.t1 AT5G39290 48.963 241 112 5 28 262 44 279 1.48e-76 234
MS.gene20357.t1 AT3G29030 50.901 222 98 4 37 257 38 249 2.69e-74 227
MS.gene20357.t1 AT5G39310 47.845 232 110 6 35 262 88 312 3.74e-73 226
MS.gene20357.t1 AT3G03220 44.643 224 123 1 36 259 39 261 1.08e-67 211
MS.gene20357.t1 AT3G15370 48.246 228 106 6 37 259 27 247 2.90e-66 206
MS.gene20357.t1 AT1G20190 51.031 194 90 3 72 262 2 193 1.68e-65 202
MS.gene20357.t1 AT3G15370 48.815 211 96 6 54 259 74 277 5.00e-60 191
MS.gene20357.t1 AT4G38210 40.948 232 132 4 35 263 26 255 2.16e-59 189
MS.gene20357.t1 AT5G39260 40.530 264 134 8 9 262 12 262 8.99e-57 182
MS.gene20357.t1 AT4G28250 33.058 242 133 8 35 262 34 260 1.08e-33 123
MS.gene20357.t1 AT1G65680 33.865 251 137 9 27 262 37 273 1.08e-32 120
MS.gene20357.t1 AT4G28250 32.645 242 128 8 35 262 34 254 2.02e-31 117
MS.gene20357.t1 AT2G20750 32.231 242 138 9 35 262 38 267 9.08e-31 115
MS.gene20357.t1 AT2G45110 31.343 268 150 8 10 262 11 259 9.91e-28 107
MS.gene20357.t1 AT1G65681 33.632 223 123 8 53 263 14 223 3.02e-25 100
MS.gene20357.t1 AT3G60570 26.692 266 167 7 9 262 8 257 3.17e-21 90.1
MS.gene20357.t1 AT4G28250 31.073 177 90 6 35 199 34 190 3.95e-18 80.9
MS.gene20357.t1 AT4G28250 30.556 180 93 6 35 202 34 193 5.51e-18 80.5
MS.gene20357.t1 AT2G20750 29.944 177 101 7 35 199 38 203 1.35e-17 79.7
MS.gene20357.t1 AT4G17030 23.735 257 179 7 11 260 1 247 8.77e-17 77.8
MS.gene20357.t1 AT3G60570 26.818 220 135 6 55 262 4 209 1.30e-16 76.6
MS.gene20357.t1 AT3G45970 25.463 216 143 7 56 262 15 221 4.66e-12 64.7
MS.gene20357.t1 AT4G38400 26.066 211 139 8 60 262 47 248 1.36e-11 63.5

Find 81 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGTCAAGCTCTTTCTTTC+AGG 0.154213 5.1:-4754852 MS.gene20357:CDS
GCAATGATGTTCACTTTCAT+TGG 0.304434 5.1:-4755748 MS.gene20357:CDS
CCAATCTTTGCCCTCCTAAT+TGG 0.315819 5.1:-4755272 MS.gene20357:CDS
CTTTCCAAGGAAACGGATAC+TGG 0.324679 5.1:-4755005 MS.gene20357:CDS
AGCAGTTTCATCTCCATAAA+AGG 0.326533 5.1:+4755666 None:intergenic
TTGATTGGTAGCATTTAATC+TGG 0.327109 5.1:+4755337 None:intergenic
GGATTAAGATGAGCCATAAC+TGG 0.340784 5.1:-4754906 MS.gene20357:CDS
ATTTACCTGCGGTACATAAC+AGG 0.349515 5.1:+4755144 None:intergenic
TCAGAGGGTTTGGACCAATT+AGG 0.358998 5.1:+4755258 None:intergenic
TCCACCATTATCAGAGGGTT+TGG 0.384127 5.1:+4755248 None:intergenic
TGGTCCAAACCCTCTGATAA+TGG 0.386369 5.1:-4755252 MS.gene20357:CDS
TTCTATAACATGCACTTGAT+TGG 0.400131 5.1:+4755322 None:intergenic
AATTTGTTTCAGAATGGTTA+CGG 0.419394 5.1:-4755423 MS.gene20357:CDS
CGTTTCCTTGGAAAGAGAAT+CGG 0.432181 5.1:+4755013 None:intergenic
ATGAGCAAGAGACCATTTAC+TGG 0.447346 5.1:+4755693 None:intergenic
ATTTGTTTCAGAATGGTTAC+GGG 0.448067 5.1:-4755422 MS.gene20357:CDS
GGAATGTTGCTCCTTCCAAT+TGG 0.448353 5.1:-4754792 MS.gene20357:CDS
GTGATGAACGTCGGAGGTGG+AGG 0.449688 5.1:-4754970 MS.gene20357:CDS
ATGGTGAAAGGAAGCAGAAC+AGG 0.453444 5.1:-4754931 MS.gene20357:CDS
GGTAGGAAGCACCCCAGTTA+TGG 0.453717 5.1:+4754893 None:intergenic
AGTGAGTCCCACATTCCAAT+TGG 0.458274 5.1:+4754777 None:intergenic
CGATTCTCTTTCCAAGGAAA+CGG 0.460365 5.1:-4755012 MS.gene20357:CDS
ATAGCCAGTATCCGTTTCCT+TGG 0.460878 5.1:+4755001 None:intergenic
CCATGCAATAATAGTCTCCT+TGG 0.461696 5.1:+4754813 None:intergenic
TACGGAAATTTGTTTCAGAA+TGG 0.468116 5.1:-4755429 MS.gene20357:CDS
CGATATTTAGACCCAGTAAA+TGG 0.471376 5.1:-4755705 MS.gene20357:CDS
TACGTGATGAACGTCGGAGG+TGG 0.485202 5.1:-4754973 MS.gene20357:CDS
TCAGTGGAAGTTGACCCTAC+TGG 0.496511 5.1:+4754750 None:intergenic
AAAATTGCACAATGGAAAGC+TGG 0.510005 5.1:-4755174 MS.gene20357:CDS
CCAATTAGGAGGGCAAAGAT+TGG 0.512665 5.1:+4755272 None:intergenic
TGCAATAATAGTCTCCTTGG+TGG 0.513313 5.1:+4754816 None:intergenic
GCTCCTTCCAATTGGAATGT+GGG 0.514369 5.1:-4754784 MS.gene20357:CDS
TGAAACTGCTTCTGAAACCA+TGG 0.519577 5.1:-4755653 MS.gene20357:CDS
ATAGGAGGAGCATGTGGATA+CGG 0.528346 5.1:-4755447 MS.gene20357:intron
AGGGTGCCATGCCAAAGAAA+AGG 0.533175 5.1:-4755042 MS.gene20357:CDS
CACCTAGTGTTGCAAATGCT+TGG 0.543954 5.1:+4754872 None:intergenic
TCAACAATGGTTATGCGTGC+GGG 0.548343 5.1:-4755368 MS.gene20357:CDS
TTCAACAATGGTTATGCGTG+CGG 0.553756 5.1:-4755369 MS.gene20357:CDS
TAGTGTTGCAAATGCTTGGT+AGG 0.556322 5.1:+4754876 None:intergenic
GATTAAGATGAGCCATAACT+GGG 0.557111 5.1:-4754905 MS.gene20357:CDS
GGAATCCGATTCTCTTTCCA+AGG 0.558029 5.1:-4755018 MS.gene20357:CDS
GAGGGTTTGGACCAATTAGG+AGG 0.565605 5.1:+4755261 None:intergenic
CACCATCCACCATTATCAGA+GGG 0.568584 5.1:+4755243 None:intergenic
TCGTCCCTGTTATGTACCGC+AGG 0.572591 5.1:-4755149 MS.gene20357:intron
AGCTCGACGTTGTTCAACAA+TGG 0.574107 5.1:-4755381 MS.gene20357:CDS
GAATGGTTACGGGACAGATA+CGG 0.574566 5.1:-4755412 MS.gene20357:CDS
GGTGGCGGTGACAGTGGTGA+AGG 0.578310 5.1:+4755293 None:intergenic
ATTAGGAGGGCAAAGATTGG+TGG 0.578409 5.1:+4755275 None:intergenic
TGGGACTCACTTATTCCAGT+AGG 0.581986 5.1:-4754765 MS.gene20357:CDS
TGCTCCTTCCAATTGGAATG+TGG 0.582320 5.1:-4754785 MS.gene20357:CDS
ATGTCGAGCATGATGGTGAA+AGG 0.595570 5.1:-4754943 MS.gene20357:CDS
AGTCCCACATTCCAATTGGA+AGG 0.604827 5.1:+4754781 None:intergenic
AGGAGGGCAAAGATTGGTGG+CGG 0.613908 5.1:+4755278 None:intergenic
ATGTCAAAATGAACTCGAGG+TGG 0.616294 5.1:+4755216 None:intergenic
GAAACTGCTTCTGAAACCAT+GGG 0.617888 5.1:-4755652 MS.gene20357:intron
GGGACTCACTTATTCCAGTA+GGG 0.618858 5.1:-4754764 MS.gene20357:CDS
CCAAGGAGACTATTATTGCA+TGG 0.618930 5.1:-4754813 MS.gene20357:CDS
TTTACCTGCGGTACATAACA+GGG 0.620314 5.1:+4755145 None:intergenic
AAGTGCATGTTATAGAAATG+TGG 0.620944 5.1:-4755316 MS.gene20357:CDS
AGGAGATATGTCGAGCATGA+TGG 0.627916 5.1:-4754950 MS.gene20357:CDS
ACACCATCCACCATTATCAG+AGG 0.628985 5.1:+4755242 None:intergenic
AGGGTTTGGACCAATTAGGA+GGG 0.631610 5.1:+4755262 None:intergenic
GTGCCATGCCAAAGAAAAGG+AGG 0.634927 5.1:-4755039 MS.gene20357:CDS
TGAAAGGAAGCAGAACAGGA+TGG 0.636895 5.1:-4754927 MS.gene20357:CDS
TGGAGAACAAACAGTAGCTG+TGG 0.637218 5.1:-4755728 MS.gene20357:CDS
TGTCAAAATGAACTCGAGGT+GGG 0.638616 5.1:+4755217 None:intergenic
AAAATTATAGGAGGAGCATG+TGG 0.639946 5.1:-4755453 MS.gene20357:intron
TGAGCAAGAGACCATTTACT+GGG 0.649236 5.1:+4755694 None:intergenic
GCCATGTCAAAATGAACTCG+AGG 0.653200 5.1:+4755213 None:intergenic
CAAGCATTTGCAACACTAGG+TGG 0.653587 5.1:-4754871 MS.gene20357:CDS
AAGATTGGTGGCGGTGACAG+TGG 0.654167 5.1:+4755287 None:intergenic
TACCAAGCATTTGCAACACT+AGG 0.658315 5.1:-4754874 MS.gene20357:CDS
TTAGTCTACGTGATGAACGT+CGG 0.658332 5.1:-4754979 MS.gene20357:CDS
AGTTTCATCTCCATAAAAGG+TGG 0.665867 5.1:+4755669 None:intergenic
AACCCTCTGATAATGGTGGA+TGG 0.666609 5.1:-4755245 MS.gene20357:CDS
CCTTCATGAAAATTGCACAA+TGG 0.672430 5.1:-4755182 MS.gene20357:CDS
GTCTACGTGATGAACGTCGG+AGG 0.675538 5.1:-4754976 MS.gene20357:CDS
TCCAAACCCTCTGATAATGG+TGG 0.691504 5.1:-4755249 MS.gene20357:CDS
TGGTTACGGGACAGATACGG+TGG 0.722045 5.1:-4755409 MS.gene20357:CDS
GCTTACTTCATACACCACCA+AGG 0.752925 5.1:-4754830 MS.gene20357:CDS
ATTAAGATGAGCCATAACTG+GGG 0.796701 5.1:-4754904 MS.gene20357:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCTGATTATTTCATACTA+AGG + chr5.1:4755447-4755466 None:intergenic 20.0%
!! TAAATGTATATATATACCCA+TGG + chr5.1:4754910-4754929 None:intergenic 20.0%
!!! TTTTTTTGTTAATATGGTTG+TGG - chr5.1:4755065-4755084 MS.gene20357:intron 20.0%
!! AATTTGTTTCAGAATGGTTA+CGG - chr5.1:4755120-4755139 MS.gene20357:intron 25.0%
!! TGCTTGTTGATTAATTTACA+GGG - chr5.1:4755482-4755501 MS.gene20357:intron 25.0%
AAGTGCATGTTATAGAAATG+TGG - chr5.1:4755227-4755246 MS.gene20357:CDS 30.0%
ATCAACAAGCACATTAGTTA+AGG + chr5.1:4755473-4755492 None:intergenic 30.0%
ATGGTTGTGGCTAAAATTAT+AGG - chr5.1:4755078-4755097 MS.gene20357:intron 30.0%
ATTTGTTTCAGAATGGTTAC+GGG - chr5.1:4755121-4755140 MS.gene20357:intron 30.0%
TCAACAAGCACATTAGTTAA+GGG + chr5.1:4755472-4755491 None:intergenic 30.0%
TTCTATAACATGCACTTGAT+TGG + chr5.1:4755224-4755243 None:intergenic 30.0%
! GTGCTTGTTGATTAATTTAC+AGG - chr5.1:4755481-4755500 MS.gene20357:intron 30.0%
! TACGGAAATTTGTTTCAGAA+TGG - chr5.1:4755114-4755133 MS.gene20357:intron 30.0%
! TTGATTGGTAGCATTTAATC+TGG + chr5.1:4755209-4755228 None:intergenic 30.0%
AAAATTGCACAATGGAAAGC+TGG - chr5.1:4755369-4755388 MS.gene20357:CDS 35.0%
AGCAGTTTCATCTCCATAAA+AGG + chr5.1:4754880-4754899 None:intergenic 35.0%
AGTTTCATCTCCATAAAAGG+TGG + chr5.1:4754877-4754896 None:intergenic 35.0%
ATTAAGATGAGCCATAACTG+GGG - chr5.1:4755639-4755658 MS.gene20357:intron 35.0%
CCTTCATGAAAATTGCACAA+TGG - chr5.1:4755361-4755380 MS.gene20357:CDS 35.0%
CGATATTTAGACCCAGTAAA+TGG - chr5.1:4754838-4754857 MS.gene20357:CDS 35.0%
GATTAAGATGAGCCATAACT+GGG - chr5.1:4755638-4755657 MS.gene20357:intron 35.0%
GCAATGATGTTCACTTTCAT+TGG - chr5.1:4754795-4754814 MS.gene20357:CDS 35.0%
GTTGTGGCTAAAATTATAGG+AGG - chr5.1:4755081-4755100 MS.gene20357:intron 35.0%
! AAAATTATAGGAGGAGCATG+TGG - chr5.1:4755090-4755109 MS.gene20357:intron 35.0%
! AATTTTCATGAAGGCAGGTT+TGG + chr5.1:4755355-4755374 None:intergenic 35.0%
! CCATTGTGCAATTTTCATGA+AGG + chr5.1:4755364-4755383 None:intergenic 35.0%
ATGAGCAAGAGACCATTTAC+TGG + chr5.1:4754853-4754872 None:intergenic 40.0%
ATGTCAAAATGAACTCGAGG+TGG + chr5.1:4755330-4755349 None:intergenic 40.0%
ATTTACCTGCGGTACATAAC+AGG + chr5.1:4755402-4755421 None:intergenic 40.0%
CCAAGGAGACTATTATTGCA+TGG - chr5.1:4755730-4755749 MS.gene20357:CDS 40.0%
CCATGCAATAATAGTCTCCT+TGG + chr5.1:4755733-4755752 None:intergenic 40.0%
CGATTCTCTTTCCAAGGAAA+CGG - chr5.1:4755531-4755550 MS.gene20357:intron 40.0%
CGTTTCCTTGGAAAGAGAAT+CGG + chr5.1:4755533-4755552 None:intergenic 40.0%
GGATTAAGATGAGCCATAAC+TGG - chr5.1:4755637-4755656 MS.gene20357:intron 40.0%
TACCAAGCATTTGCAACACT+AGG - chr5.1:4755669-4755688 MS.gene20357:CDS 40.0%
TAGTGTTGCAAATGCTTGGT+AGG + chr5.1:4755670-4755689 None:intergenic 40.0%
TGAGCAAGAGACCATTTACT+GGG + chr5.1:4754852-4754871 None:intergenic 40.0%
TGCAATAATAGTCTCCTTGG+TGG + chr5.1:4755730-4755749 None:intergenic 40.0%
TGTCAAAATGAACTCGAGGT+GGG + chr5.1:4755329-4755348 None:intergenic 40.0%
TTAGTCTACGTGATGAACGT+CGG - chr5.1:4755564-4755583 MS.gene20357:intron 40.0%
TTCAACAATGGTTATGCGTG+CGG - chr5.1:4755174-4755193 MS.gene20357:CDS 40.0%
TTTACCTGCGGTACATAACA+GGG + chr5.1:4755401-4755420 None:intergenic 40.0%
! ATCGGATTCCTCCTTTTCTT+TGG + chr5.1:4755515-4755534 None:intergenic 40.0%
! ATTCCTCCTTTTCTTTGGCA+TGG + chr5.1:4755510-4755529 None:intergenic 40.0%
! TGTGCAATTTTCATGAAGGC+AGG + chr5.1:4755360-4755379 None:intergenic 40.0%
!! ACCTCGAGTTCATTTTGACA+TGG - chr5.1:4755329-4755348 MS.gene20357:CDS 40.0%
!! GAAACTGCTTCTGAAACCAT+GGG - chr5.1:4754891-4754910 MS.gene20357:CDS 40.0%
!! TGAAACTGCTTCTGAAACCA+TGG - chr5.1:4754890-4754909 MS.gene20357:CDS 40.0%
ACACCATCCACCATTATCAG+AGG + chr5.1:4755304-4755323 None:intergenic 45.0%
AGCTCGACGTTGTTCAACAA+TGG - chr5.1:4755162-4755181 MS.gene20357:CDS 45.0%
AGGAGATATGTCGAGCATGA+TGG - chr5.1:4755593-4755612 MS.gene20357:intron 45.0%
AGGGTTTGGACCAATTAGGA+GGG + chr5.1:4755284-4755303 None:intergenic 45.0%
AGTCCCACATTCCAATTGGA+AGG + chr5.1:4755765-4755784 None:intergenic 45.0%
AGTGAGTCCCACATTCCAAT+TGG + chr5.1:4755769-4755788 None:intergenic 45.0%
ATAGCCAGTATCCGTTTCCT+TGG + chr5.1:4755545-4755564 None:intergenic 45.0%
ATGGTGAAAGGAAGCAGAAC+AGG - chr5.1:4755612-4755631 MS.gene20357:intron 45.0%
ATTAGGAGGGCAAAGATTGG+TGG + chr5.1:4755271-4755290 None:intergenic 45.0%
CAAGCATTTGCAACACTAGG+TGG - chr5.1:4755672-4755691 MS.gene20357:CDS 45.0%
CACCATCCACCATTATCAGA+GGG + chr5.1:4755303-4755322 None:intergenic 45.0%
CACCTAGTGTTGCAAATGCT+TGG + chr5.1:4755674-4755693 None:intergenic 45.0%
CCAATCTTTGCCCTCCTAAT+TGG - chr5.1:4755271-4755290 MS.gene20357:CDS 45.0%
CCAATTAGGAGGGCAAAGAT+TGG + chr5.1:4755274-4755293 None:intergenic 45.0%
CTTTCCAAGGAAACGGATAC+TGG - chr5.1:4755538-4755557 MS.gene20357:intron 45.0%
GAATGGTTACGGGACAGATA+CGG - chr5.1:4755131-4755150 MS.gene20357:intron 45.0%
GCCATGTCAAAATGAACTCG+AGG + chr5.1:4755333-4755352 None:intergenic 45.0%
GCGCATTGTGAATTTACCTG+CGG + chr5.1:4755413-4755432 None:intergenic 45.0%
GCTCCTTCCAATTGGAATGT+GGG - chr5.1:4755759-4755778 MS.gene20357:CDS 45.0%
GCTTACTTCATACACCACCA+AGG - chr5.1:4755713-4755732 MS.gene20357:CDS 45.0%
GGAATCCGATTCTCTTTCCA+AGG - chr5.1:4755525-4755544 MS.gene20357:intron 45.0%
GGAATGTTGCTCCTTCCAAT+TGG - chr5.1:4755751-4755770 MS.gene20357:CDS 45.0%
GGGACTCACTTATTCCAGTA+GGG - chr5.1:4755779-4755798 MS.gene20357:CDS 45.0%
GTGGTCAAGCTCTTTCTTTC+AGG - chr5.1:4755691-4755710 MS.gene20357:CDS 45.0%
TCAACAATGGTTATGCGTGC+GGG - chr5.1:4755175-4755194 MS.gene20357:CDS 45.0%
TCAGAGGGTTTGGACCAATT+AGG + chr5.1:4755288-4755307 None:intergenic 45.0%
TCCACCATTATCAGAGGGTT+TGG + chr5.1:4755298-4755317 None:intergenic 45.0%
TGAAAGGAAGCAGAACAGGA+TGG - chr5.1:4755616-4755635 MS.gene20357:intron 45.0%
TGCTCCTTCCAATTGGAATG+TGG - chr5.1:4755758-4755777 MS.gene20357:CDS 45.0%
TGGAGAACAAACAGTAGCTG+TGG - chr5.1:4754815-4754834 MS.gene20357:CDS 45.0%
TGGGACTCACTTATTCCAGT+AGG - chr5.1:4755778-4755797 MS.gene20357:CDS 45.0%
TGGTCCAAACCCTCTGATAA+TGG - chr5.1:4755291-4755310 MS.gene20357:CDS 45.0%
! ATAGGAGGAGCATGTGGATA+CGG - chr5.1:4755096-4755115 MS.gene20357:intron 45.0%
! ATGTCGAGCATGATGGTGAA+AGG - chr5.1:4755600-4755619 MS.gene20357:intron 45.0%
! CTTGCTCATGCCACCTTTTA+TGG - chr5.1:4754864-4754883 MS.gene20357:CDS 45.0%
!! AACCCTCTGATAATGGTGGA+TGG - chr5.1:4755298-4755317 MS.gene20357:CDS 45.0%
!! TCCAAACCCTCTGATAATGG+TGG - chr5.1:4755294-4755313 MS.gene20357:CDS 45.0%
!!! ATATTTTTTTTTTGTTAATA+TGG - chr5.1:4755059-4755078 MS.gene20357:intron 5.0%
GAGGGTTTGGACCAATTAGG+AGG + chr5.1:4755285-4755304 None:intergenic 50.0%
GTGCCATGCCAAAGAAAAGG+AGG - chr5.1:4755504-4755523 MS.gene20357:intron 50.0%
TCAGTGGAAGTTGACCCTAC+TGG + chr5.1:4755796-4755815 None:intergenic 50.0%
! AGGGTGCCATGCCAAAGAAA+AGG - chr5.1:4755501-4755520 MS.gene20357:intron 50.0%
AGGAGGGCAAAGATTGGTGG+CGG + chr5.1:4755268-4755287 None:intergenic 55.0%
GTCTACGTGATGAACGTCGG+AGG - chr5.1:4755567-4755586 MS.gene20357:intron 55.0%
TACGTGATGAACGTCGGAGG+TGG - chr5.1:4755570-4755589 MS.gene20357:intron 55.0%
TCGTCCCTGTTATGTACCGC+AGG - chr5.1:4755394-4755413 MS.gene20357:CDS 55.0%
TGGTTACGGGACAGATACGG+TGG - chr5.1:4755134-4755153 MS.gene20357:intron 55.0%
!! AAGATTGGTGGCGGTGACAG+TGG + chr5.1:4755259-4755278 None:intergenic 55.0%
!! GGTAGGAAGCACCCCAGTTA+TGG + chr5.1:4755653-4755672 None:intergenic 55.0%
GTGATGAACGTCGGAGGTGG+AGG - chr5.1:4755573-4755592 MS.gene20357:intron 60.0%
! GGTGGCGGTGACAGTGGTGA+AGG + chr5.1:4755253-4755272 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 4754750 4755815 4754750 ID=MS.gene20357
chr5.1 mRNA 4754750 4755815 4754750 ID=MS.gene20357.t1;Parent=MS.gene20357
chr5.1 exon 4755653 4755815 4755653 ID=MS.gene20357.t1.exon1;Parent=MS.gene20357.t1
chr5.1 CDS 4755653 4755815 4755653 ID=cds.MS.gene20357.t1;Parent=MS.gene20357.t1
chr5.1 exon 4755150 4755465 4755150 ID=MS.gene20357.t1.exon2;Parent=MS.gene20357.t1
chr5.1 CDS 4755150 4755465 4755150 ID=cds.MS.gene20357.t1;Parent=MS.gene20357.t1
chr5.1 exon 4754750 4755062 4754750 ID=MS.gene20357.t1.exon3;Parent=MS.gene20357.t1
chr5.1 CDS 4754750 4755062 4754750 ID=cds.MS.gene20357.t1;Parent=MS.gene20357.t1
Gene Sequence

>MS.gene20357

ATGGCATTTATTCTTCAATCTTGTTGCTTCATGTTGATTGCTTATGCAATGATGTTCACTTTCATTGGAGAACAAACAGTAGCTGTGGCGATATTTAGACCCAGTAAATGGTCTCTTGCTCATGCCACCTTTTATGGAGATGAAACTGCTTCTGAAACCATGGGTATATATATACATTTATATGTTATCTCAATTATCATTACTTCTCACTTAGCTTAATTAAGTTCAGCATTATTAGTATCATTCAACATCTCACAACTTCAATTTATTAATTAAATTAAACTACTTTTACTTAGTATCAATATTGATATATTTTTTTTTTGTTAATATGGTTGTGGCTAAAATTATAGGAGGAGCATGTGGATACGGAAATTTGTTTCAGAATGGTTACGGGACAGATACGGTGGCTTTAAGCTCGACGTTGTTCAACAATGGTTATGCGTGCGGGACATGTTTCCAGATTAAATGCTACCAATCAAGTGCATGTTATAGAAATGTGGCCTTCACCACTGTCACCGCCACCAATCTTTGCCCTCCTAATTGGTCCAAACCCTCTGATAATGGTGGATGGTGTAACCCACCTCGAGTTCATTTTGACATGGCCAAACCTGCCTTCATGAAAATTGCACAATGGAAAGCTGGTATCGTCCCTGTTATGTACCGCAGGTAAATTCACAATGCGCATTTTAACTTTCCTTAGTATGAAATAATCAGATTTTCCCTTAACTAATGTGCTTGTTGATTAATTTACAGGGTGCCATGCCAAAGAAAAGGAGGAATCCGATTCTCTTTCCAAGGAAACGGATACTGGCTATTAGTCTACGTGATGAACGTCGGAGGTGGAGGAGATATGTCGAGCATGATGGTGAAAGGAAGCAGAACAGGATGGATTAAGATGAGCCATAACTGGGGTGCTTCCTACCAAGCATTTGCAACACTAGGTGGTCAAGCTCTTTCTTTCAGGCTTACTTCATACACCACCAAGGAGACTATTATTGCATGGAATGTTGCTCCTTCCAATTGGAATGTGGGACTCACTTATTCCAGTAGGGTCAACTTCCACTGA
Protein sequence

>MS.gene20357.t1

MAFILQSCCFMLIAYAMMFTFIGEQTVAVAIFRPSKWSLAHATFYGDETASETMGGACGYGNLFQNGYGTDTVALSSTLFNNGYACGTCFQIKCYQSSACYRNVAFTTVTATNLCPPNWSKPSDNGGWCNPPRVHFDMAKPAFMKIAQWKAGIVPVMYRRVPCQRKGGIRFSFQGNGYWLLVYVMNVGGGGDMSSMMVKGSRTGWIKMSHNWGASYQAFATLGGQALSFRLTSYTTKETIIAWNVAPSNWNVGLTYSSRVNFH