AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025741

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025741.t1 MTR_5g011210 97.338 263 7 0 1 263 1 263 0.0 536
MS.gene025741.t1 MTR_4g102450 79.087 263 54 1 1 263 1 262 2.69e-157 438
MS.gene025741.t1 MTR_5g079950 55.217 230 101 2 31 259 27 255 8.53e-93 274
MS.gene025741.t1 MTR_2g097030 53.680 231 105 2 30 259 27 256 1.83e-91 271
MS.gene025741.t1 MTR_5g041700 58.108 222 89 3 37 257 25 243 3.01e-90 267
MS.gene025741.t1 MTR_7g061720 50.794 252 121 3 10 259 4 254 1.03e-89 266
MS.gene025741.t1 MTR_1g085460 54.348 230 99 4 37 262 30 257 1.02e-87 261
MS.gene025741.t1 MTR_3g094060 56.054 223 96 2 37 259 31 251 5.49e-87 259
MS.gene025741.t1 MTR_7g111010 51.550 258 111 6 9 262 7 254 7.06e-87 259
MS.gene025741.t1 MTR_4g081950 55.405 222 95 3 37 257 61 279 1.43e-86 259
MS.gene025741.t1 MTR_7g110990 55.217 230 97 4 37 262 12 239 2.05e-86 257
MS.gene025741.t1 MTR_3g094040 56.109 221 95 2 37 257 31 249 3.18e-86 257
MS.gene025741.t1 MTR_4g063975 50.000 250 123 2 11 259 6 254 4.04e-86 257
MS.gene025741.t1 MTR_8g464350 50.000 258 122 5 6 262 3 254 7.28e-86 256
MS.gene025741.t1 MTR_3g435430 53.648 233 103 4 27 257 14 243 8.95e-86 256
MS.gene025741.t1 MTR_5g075320 51.037 241 116 2 16 256 8 246 2.61e-85 255
MS.gene025741.t1 MTR_1g026020 50.000 240 115 4 26 262 18 255 1.38e-83 251
MS.gene025741.t1 MTR_8g058990 53.913 230 100 4 35 262 28 253 2.86e-83 250
MS.gene025741.t1 MTR_7g013140 54.825 228 99 3 36 262 27 251 4.20e-83 249
MS.gene025741.t1 MTR_7g013150 48.638 257 124 4 7 262 4 253 3.50e-81 244
MS.gene025741.t1 MTR_7g013120 52.193 228 104 4 37 262 29 253 6.11e-81 244
MS.gene025741.t1 MTR_7g011060 52.444 225 97 3 35 259 24 238 2.82e-80 242
MS.gene025741.t1 MTR_3g466760 51.639 244 116 2 13 256 14 255 4.05e-80 242
MS.gene025741.t1 MTR_3g466790 51.230 244 117 2 13 256 8 249 2.76e-79 239
MS.gene025741.t1 MTR_4g124340 49.333 225 109 4 37 259 61 282 3.52e-78 238
MS.gene025741.t1 MTR_6g012520 49.231 260 108 5 1 259 4 240 2.55e-70 216
MS.gene025741.t1 MTR_0040s0140 41.935 248 139 3 16 259 8 254 3.20e-68 211
MS.gene025741.t1 MTR_5g076950 45.778 225 121 1 35 259 39 262 2.08e-67 210
MS.gene025741.t1 MTR_2g090255 39.506 243 145 2 18 259 9 250 5.07e-64 201
MS.gene025741.t1 MTR_3g064510 43.777 233 122 5 35 259 51 282 1.53e-60 193
MS.gene025741.t1 MTR_0008s0120 36.576 257 103 4 6 262 3 199 1.13e-50 165
MS.gene025741.t1 MTR_2g090270 31.868 273 150 11 3 262 9 258 1.07e-31 117
MS.gene025741.t1 MTR_0122s0020 33.333 240 132 10 37 263 41 265 1.86e-30 114
MS.gene025741.t1 MTR_2g090265 31.111 270 157 11 6 262 3 256 2.78e-30 114
MS.gene025741.t1 MTR_4g028640 30.877 285 155 12 1 263 1 265 1.51e-27 107
MS.gene025741.t1 MTR_6g012500 48.485 99 50 1 161 259 181 278 4.51e-25 100
MS.gene025741.t1 MTR_0118s0070 29.958 237 136 9 42 262 53 275 1.24e-21 91.7
MS.gene025741.t1 MTR_0809s0010 28.685 251 145 9 28 262 38 270 7.78e-20 86.7
MS.gene025741.t1 MTR_5g013440 25.490 255 157 7 27 263 13 252 4.43e-19 84.3
MS.gene025741.t1 MTR_0122s0020 32.584 178 94 8 37 202 41 204 9.13e-19 82.4
MS.gene025741.t1 MTR_4g075790 28.634 227 131 6 50 262 34 243 1.31e-15 74.7
MS.gene025741.t1 MTR_4g099370 25.652 230 156 5 40 263 31 251 6.70e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025741.t1 AT1G12560 68.826 247 74 2 17 263 19 262 1.96e-126 360
MS.gene025741.t1 AT1G62980 63.320 259 89 2 5 263 5 257 3.25e-123 351
MS.gene025741.t1 AT2G39700 54.582 251 108 3 11 256 1 250 1.73e-95 281
MS.gene025741.t1 AT3G55500 51.807 249 117 3 10 256 6 253 1.02e-92 274
MS.gene025741.t1 AT2G37640 50.973 257 123 3 8 263 7 261 7.84e-91 270
MS.gene025741.t1 AT2G03090 56.951 223 94 2 37 259 30 250 9.41e-89 264
MS.gene025741.t1 AT1G69530 52.227 247 116 2 11 257 1 245 2.15e-88 263
MS.gene025741.t1 AT1G69530 52.227 247 116 2 11 257 1 245 2.15e-88 263
MS.gene025741.t1 AT1G69530 52.227 247 116 2 11 257 1 245 3.06e-88 263
MS.gene025741.t1 AT5G02260 49.219 256 127 3 5 259 1 254 4.29e-88 262
MS.gene025741.t1 AT2G28950 53.043 230 106 2 31 259 25 253 4.63e-87 259
MS.gene025741.t1 AT1G20190 53.138 239 107 3 27 262 16 252 5.81e-86 257
MS.gene025741.t1 AT4G01630 53.478 230 101 4 37 262 28 255 3.18e-85 255
MS.gene025741.t1 AT5G56320 50.400 250 116 4 11 259 7 249 1.22e-84 253
MS.gene025741.t1 AT5G56320 50.400 250 116 4 11 259 7 249 1.22e-84 253
MS.gene025741.t1 AT5G56320 50.400 250 116 4 11 259 7 249 6.20e-84 253
MS.gene025741.t1 AT2G40610 54.955 222 95 4 37 256 30 248 2.52e-83 250
MS.gene025741.t1 AT2G37640 54.976 211 92 3 54 263 28 236 5.40e-82 246
MS.gene025741.t1 AT5G05290 51.351 222 105 3 37 257 32 251 5.23e-80 241
MS.gene025741.t1 AT1G69530 50.855 234 110 3 11 244 1 229 4.49e-79 239
MS.gene025741.t1 AT1G69530 54.286 210 95 1 11 220 1 209 1.38e-78 237
MS.gene025741.t1 AT1G26770 55.204 221 97 2 37 257 26 244 2.32e-78 237
MS.gene025741.t1 AT1G26770 55.204 221 97 2 37 257 36 254 2.97e-78 237
MS.gene025741.t1 AT5G39300 45.387 271 127 6 8 262 11 276 3.16e-78 238
MS.gene025741.t1 AT5G39270 49.378 241 111 5 28 262 44 279 4.36e-78 238
MS.gene025741.t1 AT5G39280 46.442 267 130 6 1 262 17 275 6.07e-78 237
MS.gene025741.t1 AT5G39290 48.963 241 112 5 28 262 44 279 1.45e-76 234
MS.gene025741.t1 AT3G29030 50.901 222 98 4 37 257 38 249 1.99e-74 228
MS.gene025741.t1 AT5G39310 47.845 232 110 6 35 262 88 312 2.67e-73 226
MS.gene025741.t1 AT3G03220 44.643 224 123 1 36 259 39 261 1.05e-67 211
MS.gene025741.t1 AT3G15370 48.246 228 106 6 37 259 27 247 2.96e-66 206
MS.gene025741.t1 AT1G20190 51.031 194 90 3 72 262 2 193 1.49e-65 202
MS.gene025741.t1 AT3G15370 48.815 211 96 6 54 259 74 277 5.63e-60 191
MS.gene025741.t1 AT4G38210 40.948 232 132 4 35 263 26 255 2.14e-59 189
MS.gene025741.t1 AT5G39260 40.377 265 135 8 8 262 11 262 5.71e-57 183
MS.gene025741.t1 AT4G28250 33.058 242 133 8 35 262 34 260 1.20e-33 123
MS.gene025741.t1 AT1G65680 33.865 251 137 9 27 262 37 273 1.01e-32 120
MS.gene025741.t1 AT4G28250 32.645 242 128 8 35 262 34 254 1.73e-31 117
MS.gene025741.t1 AT2G20750 32.231 242 138 9 35 262 38 267 8.44e-31 115
MS.gene025741.t1 AT2G45110 31.343 268 150 8 10 262 11 259 7.87e-28 107
MS.gene025741.t1 AT1G65681 33.784 222 122 8 53 262 14 222 2.87e-25 100
MS.gene025741.t1 AT3G60570 26.592 267 168 7 8 262 7 257 1.20e-21 91.3
MS.gene025741.t1 AT4G28250 31.073 177 90 6 35 199 34 190 4.42e-18 80.5
MS.gene025741.t1 AT4G28250 30.556 180 93 6 35 202 34 193 5.19e-18 80.5
MS.gene025741.t1 AT2G20750 29.944 177 101 7 35 199 38 203 1.42e-17 79.3
MS.gene025741.t1 AT4G17030 23.735 257 179 7 11 260 1 247 6.89e-17 78.2
MS.gene025741.t1 AT3G60570 26.818 220 135 6 55 262 4 209 1.25e-16 76.6
MS.gene025741.t1 AT3G45970 25.463 216 143 7 56 262 15 221 4.99e-12 64.3
MS.gene025741.t1 AT4G38400 26.066 211 139 8 60 262 47 248 1.58e-11 63.5

Find 81 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGTCAAGCTCTTTCTTTC+AGG 0.154213 5.3:-4831331 MS.gene025741:CDS
GCAATGATGTTCACTTTCAT+TGG 0.304434 5.3:-4832227 MS.gene025741:CDS
CCAATCTTTGCCCTCCTAAT+TGG 0.315819 5.3:-4831751 MS.gene025741:CDS
CTTTCCAAGGAAACGGATAC+TGG 0.324679 5.3:-4831484 MS.gene025741:CDS
AGCAGTTTCATCTCCATAAA+AGG 0.326533 5.3:+4832145 None:intergenic
TTGATTGGTAGCATTTAATC+TGG 0.327109 5.3:+4831816 None:intergenic
GGATTAAGATGAGCCATAAC+TGG 0.340784 5.3:-4831385 MS.gene025741:CDS
ATTTACCTGCGGTACATAAC+AGG 0.349515 5.3:+4831623 None:intergenic
TCAGAGGGTTTGGACCAATT+AGG 0.358998 5.3:+4831737 None:intergenic
TCCACCATTATCAGAGGGTT+TGG 0.384127 5.3:+4831727 None:intergenic
TGGTCCAAACCCTCTGATAA+TGG 0.386369 5.3:-4831731 MS.gene025741:CDS
TTCTATAACATGCACTTGAT+TGG 0.400131 5.3:+4831801 None:intergenic
AATTTGTTTCAGAATGGTTA+CGG 0.419394 5.3:-4831902 MS.gene025741:CDS
CGTTTCCTTGGAAAGAGAAT+CGG 0.432181 5.3:+4831492 None:intergenic
ATGAGCAAGAGACCATTTAC+TGG 0.447346 5.3:+4832172 None:intergenic
ATTTGTTTCAGAATGGTTAC+GGG 0.448067 5.3:-4831901 MS.gene025741:CDS
GGAATGTTGCTCCTTCCAAT+TGG 0.448353 5.3:-4831271 MS.gene025741:CDS
GTGATGAACGTCGGAGGTGG+AGG 0.449688 5.3:-4831449 MS.gene025741:CDS
ATGGTGAAAGGAAGCAGAAC+AGG 0.453444 5.3:-4831410 MS.gene025741:CDS
GGTAGGAAGCACCCCAGTTA+TGG 0.453717 5.3:+4831372 None:intergenic
AGTGAGTCCCACATTCCAAT+TGG 0.458274 5.3:+4831256 None:intergenic
CGATTCTCTTTCCAAGGAAA+CGG 0.460365 5.3:-4831491 MS.gene025741:CDS
ATAGCCAGTATCCGTTTCCT+TGG 0.460878 5.3:+4831480 None:intergenic
CCATGCAATAATAGTCTCCT+TGG 0.461696 5.3:+4831292 None:intergenic
TACGGAAATTTGTTTCAGAA+TGG 0.468116 5.3:-4831908 MS.gene025741:CDS
CGATATTTAGACCCAGTAAA+TGG 0.471376 5.3:-4832184 MS.gene025741:CDS
TACGTGATGAACGTCGGAGG+TGG 0.485202 5.3:-4831452 MS.gene025741:CDS
TCAGTGGAAGTTGACCCTAC+TGG 0.496511 5.3:+4831229 None:intergenic
AAAATTGCACAATGGAAAGC+TGG 0.510005 5.3:-4831653 MS.gene025741:CDS
CCAATTAGGAGGGCAAAGAT+TGG 0.512665 5.3:+4831751 None:intergenic
TGCAATAATAGTCTCCTTGG+TGG 0.513313 5.3:+4831295 None:intergenic
GCTCCTTCCAATTGGAATGT+GGG 0.514369 5.3:-4831263 MS.gene025741:CDS
TGAAACTGCTTCTGAAACCA+TGG 0.519577 5.3:-4832132 MS.gene025741:CDS
ATAGGAGGAGCATGTGGATA+CGG 0.528346 5.3:-4831926 MS.gene025741:intron
AGGGTGCCATGCCAAAGAAA+AGG 0.533175 5.3:-4831521 MS.gene025741:CDS
CACCTAGTGTTGCAAATGCT+TGG 0.543954 5.3:+4831351 None:intergenic
TCAACAATGGTTATGCGTGC+GGG 0.548343 5.3:-4831847 MS.gene025741:CDS
TTCAACAATGGTTATGCGTG+CGG 0.553756 5.3:-4831848 MS.gene025741:CDS
TAGTGTTGCAAATGCTTGGT+AGG 0.556322 5.3:+4831355 None:intergenic
GATTAAGATGAGCCATAACT+GGG 0.557111 5.3:-4831384 MS.gene025741:CDS
GGAATCCGATTCTCTTTCCA+AGG 0.558029 5.3:-4831497 MS.gene025741:CDS
GAGGGTTTGGACCAATTAGG+AGG 0.565605 5.3:+4831740 None:intergenic
CACCATCCACCATTATCAGA+GGG 0.568584 5.3:+4831722 None:intergenic
TCGTCCCTGTTATGTACCGC+AGG 0.572591 5.3:-4831628 MS.gene025741:intron
AGCTCGACGTTGTTCAACAA+TGG 0.574107 5.3:-4831860 MS.gene025741:CDS
GAATGGTTACGGGACAGATA+CGG 0.574566 5.3:-4831891 MS.gene025741:CDS
GGTGGCGGTGACAGTGGTGA+AGG 0.578310 5.3:+4831772 None:intergenic
ATTAGGAGGGCAAAGATTGG+TGG 0.578409 5.3:+4831754 None:intergenic
TGGGACTCACTTATTCCAGT+AGG 0.581986 5.3:-4831244 MS.gene025741:CDS
TGCTCCTTCCAATTGGAATG+TGG 0.582320 5.3:-4831264 MS.gene025741:CDS
ATGTCGAGCATGATGGTGAA+AGG 0.595570 5.3:-4831422 MS.gene025741:CDS
AGTCCCACATTCCAATTGGA+AGG 0.604827 5.3:+4831260 None:intergenic
AGGAGGGCAAAGATTGGTGG+CGG 0.613908 5.3:+4831757 None:intergenic
ATGTCAAAATGAACTCGAGG+TGG 0.616294 5.3:+4831695 None:intergenic
GAAACTGCTTCTGAAACCAT+GGG 0.617888 5.3:-4832131 MS.gene025741:intron
GGGACTCACTTATTCCAGTA+GGG 0.618858 5.3:-4831243 MS.gene025741:CDS
CCAAGGAGACTATTATTGCA+TGG 0.618930 5.3:-4831292 MS.gene025741:CDS
TTTACCTGCGGTACATAACA+GGG 0.620314 5.3:+4831624 None:intergenic
AAGTGCATGTTATAGAAATG+TGG 0.620944 5.3:-4831795 MS.gene025741:CDS
AGGAGATATGTCGAGCATGA+TGG 0.627916 5.3:-4831429 MS.gene025741:CDS
ACACCATCCACCATTATCAG+AGG 0.628985 5.3:+4831721 None:intergenic
AGGGTTTGGACCAATTAGGA+GGG 0.631610 5.3:+4831741 None:intergenic
GTGCCATGCCAAAGAAAAGG+AGG 0.634927 5.3:-4831518 MS.gene025741:CDS
TGAAAGGAAGCAGAACAGGA+TGG 0.636895 5.3:-4831406 MS.gene025741:CDS
TGGAGAACAAACAGTAGCTG+TGG 0.637218 5.3:-4832207 MS.gene025741:CDS
TGTCAAAATGAACTCGAGGT+GGG 0.638616 5.3:+4831696 None:intergenic
AAAATTATAGGAGGAGCATG+TGG 0.639946 5.3:-4831932 MS.gene025741:intron
TGAGCAAGAGACCATTTACT+GGG 0.649236 5.3:+4832173 None:intergenic
GCCATGTCAAAATGAACTCG+AGG 0.653200 5.3:+4831692 None:intergenic
CAAGCATTTGCAACACTAGG+TGG 0.653587 5.3:-4831350 MS.gene025741:CDS
AAGATTGGTGGCGGTGACAG+TGG 0.654167 5.3:+4831766 None:intergenic
TACCAAGCATTTGCAACACT+AGG 0.658315 5.3:-4831353 MS.gene025741:CDS
TTAGTCTACGTGATGAACGT+CGG 0.658332 5.3:-4831458 MS.gene025741:CDS
AGTTTCATCTCCATAAAAGG+TGG 0.665867 5.3:+4832148 None:intergenic
AACCCTCTGATAATGGTGGA+TGG 0.666609 5.3:-4831724 MS.gene025741:CDS
CCTTCATGAAAATTGCACAA+TGG 0.672430 5.3:-4831661 MS.gene025741:CDS
GTCTACGTGATGAACGTCGG+AGG 0.675538 5.3:-4831455 MS.gene025741:CDS
TCCAAACCCTCTGATAATGG+TGG 0.691504 5.3:-4831728 MS.gene025741:CDS
TGGTTACGGGACAGATACGG+TGG 0.722045 5.3:-4831888 MS.gene025741:CDS
GCTTACTTCATACACCACCA+AGG 0.752925 5.3:-4831309 MS.gene025741:CDS
ATTAAGATGAGCCATAACTG+GGG 0.796701 5.3:-4831383 MS.gene025741:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCTGATTATTTCATACTA+AGG + chr5.3:4831926-4831945 None:intergenic 20.0%
!! TAAATGTATATATATACCCA+TGG + chr5.3:4831389-4831408 None:intergenic 20.0%
!!! TTTTTTTGTTAATATGGTTG+TGG - chr5.3:4831544-4831563 MS.gene025741:intron 20.0%
!! AATTTGTTTCAGAATGGTTA+CGG - chr5.3:4831599-4831618 MS.gene025741:intron 25.0%
!! TGCTTGTTGATTAATTTACA+GGG - chr5.3:4831961-4831980 MS.gene025741:intron 25.0%
AAGTGCATGTTATAGAAATG+TGG - chr5.3:4831706-4831725 MS.gene025741:CDS 30.0%
ATCAACAAGCACATTAGTTA+AGG + chr5.3:4831952-4831971 None:intergenic 30.0%
ATGGTTGTGGCTAAAATTAT+AGG - chr5.3:4831557-4831576 MS.gene025741:intron 30.0%
ATTTGTTTCAGAATGGTTAC+GGG - chr5.3:4831600-4831619 MS.gene025741:intron 30.0%
TCAACAAGCACATTAGTTAA+GGG + chr5.3:4831951-4831970 None:intergenic 30.0%
TTCTATAACATGCACTTGAT+TGG + chr5.3:4831703-4831722 None:intergenic 30.0%
! GTGCTTGTTGATTAATTTAC+AGG - chr5.3:4831960-4831979 MS.gene025741:intron 30.0%
! TACGGAAATTTGTTTCAGAA+TGG - chr5.3:4831593-4831612 MS.gene025741:intron 30.0%
! TTGATTGGTAGCATTTAATC+TGG + chr5.3:4831688-4831707 None:intergenic 30.0%
AAAATTGCACAATGGAAAGC+TGG - chr5.3:4831848-4831867 MS.gene025741:CDS 35.0%
AGCAGTTTCATCTCCATAAA+AGG + chr5.3:4831359-4831378 None:intergenic 35.0%
AGTTTCATCTCCATAAAAGG+TGG + chr5.3:4831356-4831375 None:intergenic 35.0%
ATTAAGATGAGCCATAACTG+GGG - chr5.3:4832118-4832137 MS.gene025741:intron 35.0%
CCTTCATGAAAATTGCACAA+TGG - chr5.3:4831840-4831859 MS.gene025741:CDS 35.0%
CGATATTTAGACCCAGTAAA+TGG - chr5.3:4831317-4831336 MS.gene025741:CDS 35.0%
GATTAAGATGAGCCATAACT+GGG - chr5.3:4832117-4832136 MS.gene025741:intron 35.0%
GCAATGATGTTCACTTTCAT+TGG - chr5.3:4831274-4831293 MS.gene025741:CDS 35.0%
GTTGTGGCTAAAATTATAGG+AGG - chr5.3:4831560-4831579 MS.gene025741:intron 35.0%
! AAAATTATAGGAGGAGCATG+TGG - chr5.3:4831569-4831588 MS.gene025741:intron 35.0%
! AATTTTCATGAAGGCAGGTT+TGG + chr5.3:4831834-4831853 None:intergenic 35.0%
! CCATTGTGCAATTTTCATGA+AGG + chr5.3:4831843-4831862 None:intergenic 35.0%
ATGAGCAAGAGACCATTTAC+TGG + chr5.3:4831332-4831351 None:intergenic 40.0%
ATGTCAAAATGAACTCGAGG+TGG + chr5.3:4831809-4831828 None:intergenic 40.0%
ATTTACCTGCGGTACATAAC+AGG + chr5.3:4831881-4831900 None:intergenic 40.0%
CCAAGGAGACTATTATTGCA+TGG - chr5.3:4832209-4832228 MS.gene025741:CDS 40.0%
CCATGCAATAATAGTCTCCT+TGG + chr5.3:4832212-4832231 None:intergenic 40.0%
CGATTCTCTTTCCAAGGAAA+CGG - chr5.3:4832010-4832029 MS.gene025741:intron 40.0%
CGTTTCCTTGGAAAGAGAAT+CGG + chr5.3:4832012-4832031 None:intergenic 40.0%
GGATTAAGATGAGCCATAAC+TGG - chr5.3:4832116-4832135 MS.gene025741:intron 40.0%
TACCAAGCATTTGCAACACT+AGG - chr5.3:4832148-4832167 MS.gene025741:CDS 40.0%
TAGTGTTGCAAATGCTTGGT+AGG + chr5.3:4832149-4832168 None:intergenic 40.0%
TGAGCAAGAGACCATTTACT+GGG + chr5.3:4831331-4831350 None:intergenic 40.0%
TGCAATAATAGTCTCCTTGG+TGG + chr5.3:4832209-4832228 None:intergenic 40.0%
TGTCAAAATGAACTCGAGGT+GGG + chr5.3:4831808-4831827 None:intergenic 40.0%
TTAGTCTACGTGATGAACGT+CGG - chr5.3:4832043-4832062 MS.gene025741:intron 40.0%
TTCAACAATGGTTATGCGTG+CGG - chr5.3:4831653-4831672 MS.gene025741:CDS 40.0%
TTTACCTGCGGTACATAACA+GGG + chr5.3:4831880-4831899 None:intergenic 40.0%
! ATCGGATTCCTCCTTTTCTT+TGG + chr5.3:4831994-4832013 None:intergenic 40.0%
! ATTCCTCCTTTTCTTTGGCA+TGG + chr5.3:4831989-4832008 None:intergenic 40.0%
! TGTGCAATTTTCATGAAGGC+AGG + chr5.3:4831839-4831858 None:intergenic 40.0%
!! ACCTCGAGTTCATTTTGACA+TGG - chr5.3:4831808-4831827 MS.gene025741:CDS 40.0%
!! GAAACTGCTTCTGAAACCAT+GGG - chr5.3:4831370-4831389 MS.gene025741:CDS 40.0%
!! TGAAACTGCTTCTGAAACCA+TGG - chr5.3:4831369-4831388 MS.gene025741:CDS 40.0%
ACACCATCCACCATTATCAG+AGG + chr5.3:4831783-4831802 None:intergenic 45.0%
AGCTCGACGTTGTTCAACAA+TGG - chr5.3:4831641-4831660 MS.gene025741:CDS 45.0%
AGGAGATATGTCGAGCATGA+TGG - chr5.3:4832072-4832091 MS.gene025741:intron 45.0%
AGGGTTTGGACCAATTAGGA+GGG + chr5.3:4831763-4831782 None:intergenic 45.0%
AGTCCCACATTCCAATTGGA+AGG + chr5.3:4832244-4832263 None:intergenic 45.0%
AGTGAGTCCCACATTCCAAT+TGG + chr5.3:4832248-4832267 None:intergenic 45.0%
ATAGCCAGTATCCGTTTCCT+TGG + chr5.3:4832024-4832043 None:intergenic 45.0%
ATGGTGAAAGGAAGCAGAAC+AGG - chr5.3:4832091-4832110 MS.gene025741:intron 45.0%
ATTAGGAGGGCAAAGATTGG+TGG + chr5.3:4831750-4831769 None:intergenic 45.0%
CAAGCATTTGCAACACTAGG+TGG - chr5.3:4832151-4832170 MS.gene025741:CDS 45.0%
CACCATCCACCATTATCAGA+GGG + chr5.3:4831782-4831801 None:intergenic 45.0%
CACCTAGTGTTGCAAATGCT+TGG + chr5.3:4832153-4832172 None:intergenic 45.0%
CCAATCTTTGCCCTCCTAAT+TGG - chr5.3:4831750-4831769 MS.gene025741:CDS 45.0%
CCAATTAGGAGGGCAAAGAT+TGG + chr5.3:4831753-4831772 None:intergenic 45.0%
CTTTCCAAGGAAACGGATAC+TGG - chr5.3:4832017-4832036 MS.gene025741:intron 45.0%
GAATGGTTACGGGACAGATA+CGG - chr5.3:4831610-4831629 MS.gene025741:intron 45.0%
GCCATGTCAAAATGAACTCG+AGG + chr5.3:4831812-4831831 None:intergenic 45.0%
GCGCATTGTGAATTTACCTG+CGG + chr5.3:4831892-4831911 None:intergenic 45.0%
GCTCCTTCCAATTGGAATGT+GGG - chr5.3:4832238-4832257 MS.gene025741:CDS 45.0%
GCTTACTTCATACACCACCA+AGG - chr5.3:4832192-4832211 MS.gene025741:CDS 45.0%
GGAATCCGATTCTCTTTCCA+AGG - chr5.3:4832004-4832023 MS.gene025741:intron 45.0%
GGAATGTTGCTCCTTCCAAT+TGG - chr5.3:4832230-4832249 MS.gene025741:CDS 45.0%
GGGACTCACTTATTCCAGTA+GGG - chr5.3:4832258-4832277 MS.gene025741:CDS 45.0%
GTGGTCAAGCTCTTTCTTTC+AGG - chr5.3:4832170-4832189 MS.gene025741:CDS 45.0%
TCAACAATGGTTATGCGTGC+GGG - chr5.3:4831654-4831673 MS.gene025741:CDS 45.0%
TCAGAGGGTTTGGACCAATT+AGG + chr5.3:4831767-4831786 None:intergenic 45.0%
TCCACCATTATCAGAGGGTT+TGG + chr5.3:4831777-4831796 None:intergenic 45.0%
TGAAAGGAAGCAGAACAGGA+TGG - chr5.3:4832095-4832114 MS.gene025741:intron 45.0%
TGCTCCTTCCAATTGGAATG+TGG - chr5.3:4832237-4832256 MS.gene025741:CDS 45.0%
TGGAGAACAAACAGTAGCTG+TGG - chr5.3:4831294-4831313 MS.gene025741:CDS 45.0%
TGGGACTCACTTATTCCAGT+AGG - chr5.3:4832257-4832276 MS.gene025741:CDS 45.0%
TGGTCCAAACCCTCTGATAA+TGG - chr5.3:4831770-4831789 MS.gene025741:CDS 45.0%
! ATAGGAGGAGCATGTGGATA+CGG - chr5.3:4831575-4831594 MS.gene025741:intron 45.0%
! ATGTCGAGCATGATGGTGAA+AGG - chr5.3:4832079-4832098 MS.gene025741:intron 45.0%
! CTTGCTCATGCCACCTTTTA+TGG - chr5.3:4831343-4831362 MS.gene025741:CDS 45.0%
!! AACCCTCTGATAATGGTGGA+TGG - chr5.3:4831777-4831796 MS.gene025741:CDS 45.0%
!! TCCAAACCCTCTGATAATGG+TGG - chr5.3:4831773-4831792 MS.gene025741:CDS 45.0%
!!! ATATATTTTTTTTGTTAATA+TGG - chr5.3:4831538-4831557 MS.gene025741:intron 5.0%
GAGGGTTTGGACCAATTAGG+AGG + chr5.3:4831764-4831783 None:intergenic 50.0%
GTGCCATGCCAAAGAAAAGG+AGG - chr5.3:4831983-4832002 MS.gene025741:intron 50.0%
TCAGTGGAAGTTGACCCTAC+TGG + chr5.3:4832275-4832294 None:intergenic 50.0%
! AGGGTGCCATGCCAAAGAAA+AGG - chr5.3:4831980-4831999 MS.gene025741:intron 50.0%
AGGAGGGCAAAGATTGGTGG+CGG + chr5.3:4831747-4831766 None:intergenic 55.0%
GTCTACGTGATGAACGTCGG+AGG - chr5.3:4832046-4832065 MS.gene025741:intron 55.0%
TACGTGATGAACGTCGGAGG+TGG - chr5.3:4832049-4832068 MS.gene025741:intron 55.0%
TCGTCCCTGTTATGTACCGC+AGG - chr5.3:4831873-4831892 MS.gene025741:CDS 55.0%
TGGTTACGGGACAGATACGG+TGG - chr5.3:4831613-4831632 MS.gene025741:intron 55.0%
!! AAGATTGGTGGCGGTGACAG+TGG + chr5.3:4831738-4831757 None:intergenic 55.0%
!! GGTAGGAAGCACCCCAGTTA+TGG + chr5.3:4832132-4832151 None:intergenic 55.0%
GTGATGAACGTCGGAGGTGG+AGG - chr5.3:4832052-4832071 MS.gene025741:intron 60.0%
! GGTGGCGGTGACAGTGGTGA+AGG + chr5.3:4831732-4831751 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 4831229 4832294 4831229 ID=MS.gene025741
chr5.3 mRNA 4831229 4832294 4831229 ID=MS.gene025741.t1;Parent=MS.gene025741
chr5.3 exon 4832132 4832294 4832132 ID=MS.gene025741.t1.exon1;Parent=MS.gene025741.t1
chr5.3 CDS 4832132 4832294 4832132 ID=cds.MS.gene025741.t1;Parent=MS.gene025741.t1
chr5.3 exon 4831629 4831944 4831629 ID=MS.gene025741.t1.exon2;Parent=MS.gene025741.t1
chr5.3 CDS 4831629 4831944 4831629 ID=cds.MS.gene025741.t1;Parent=MS.gene025741.t1
chr5.3 exon 4831229 4831541 4831229 ID=MS.gene025741.t1.exon3;Parent=MS.gene025741.t1
chr5.3 CDS 4831229 4831541 4831229 ID=cds.MS.gene025741.t1;Parent=MS.gene025741.t1
Gene Sequence

>MS.gene025741

ATGGCATTTATTCTTCAATCTCGTTGCTTCATGTTGATTGCTTATGCAATGATGTTCACTTTCATTGGAGAACAAACAGTAGCTGTGGCGATATTTAGACCCAGTAAATGGTCTCTTGCTCATGCCACCTTTTATGGAGATGAAACTGCTTCTGAAACCATGGGTATATATATACATTTATATGTTATCTCAATTATCATTACTTCTCACTTAGCTTAATTAAGTTCAGCATTATTAGTATCATTCAACATCTCACAACTTCAATTTATTAATTAAATTAAACTACTTTTACTTAGTATCAATATTGATATATATTTTTTTTGTTAATATGGTTGTGGCTAAAATTATAGGAGGAGCATGTGGATACGGAAATTTGTTTCAGAATGGTTACGGGACAGATACGGTGGCTTTAAGCTCGACGTTGTTCAACAATGGTTATGCGTGCGGGACATGTTTCCAGATTAAATGCTACCAATCAAGTGCATGTTATAGAAATGTGGCCTTCACCACTGTCACCGCCACCAATCTTTGCCCTCCTAATTGGTCCAAACCCTCTGATAATGGTGGATGGTGTAACCCACCTCGAGTTCATTTTGACATGGCCAAACCTGCCTTCATGAAAATTGCACAATGGAAAGCTGGTATCGTCCCTGTTATGTACCGCAGGTAAATTCACAATGCGCATTTTAACTTTCCTTAGTATGAAATAATCAGATTTTCCCTTAACTAATGTGCTTGTTGATTAATTTACAGGGTGCCATGCCAAAGAAAAGGAGGAATCCGATTCTCTTTCCAAGGAAACGGATACTGGCTATTAGTCTACGTGATGAACGTCGGAGGTGGAGGAGATATGTCGAGCATGATGGTGAAAGGAAGCAGAACAGGATGGATTAAGATGAGCCATAACTGGGGTGCTTCCTACCAAGCATTTGCAACACTAGGTGGTCAAGCTCTTTCTTTCAGGCTTACTTCATACACCACCAAGGAGACTATTATTGCATGGAATGTTGCTCCTTCCAATTGGAATGTGGGACTCACTTATTCCAGTAGGGTCAACTTCCACTGA
Protein sequence

>MS.gene025741.t1

MAFILQSRCFMLIAYAMMFTFIGEQTVAVAIFRPSKWSLAHATFYGDETASETMGGACGYGNLFQNGYGTDTVALSSTLFNNGYACGTCFQIKCYQSSACYRNVAFTTVTATNLCPPNWSKPSDNGGWCNPPRVHFDMAKPAFMKIAQWKAGIVPVMYRRVPCQRKGGIRFSFQGNGYWLLVYVMNVGGGGDMSSMMVKGSRTGWIKMSHNWGASYQAFATLGGQALSFRLTSYTTKETIIAWNVAPSNWNVGLTYSSRVNFH