AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22589

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22589.t1 MTR_5g011210 97.338 263 7 0 1 263 1 263 0.0 536
MS.gene22589.t1 MTR_4g102450 79.087 263 54 1 1 263 1 262 2.69e-157 438
MS.gene22589.t1 MTR_5g079950 55.217 230 101 2 31 259 27 255 8.53e-93 274
MS.gene22589.t1 MTR_2g097030 53.680 231 105 2 30 259 27 256 1.83e-91 271
MS.gene22589.t1 MTR_5g041700 58.108 222 89 3 37 257 25 243 3.01e-90 267
MS.gene22589.t1 MTR_7g061720 50.794 252 121 3 10 259 4 254 1.03e-89 266
MS.gene22589.t1 MTR_1g085460 54.348 230 99 4 37 262 30 257 1.02e-87 261
MS.gene22589.t1 MTR_3g094060 56.054 223 96 2 37 259 31 251 5.49e-87 259
MS.gene22589.t1 MTR_7g111010 51.550 258 111 6 9 262 7 254 7.06e-87 259
MS.gene22589.t1 MTR_4g081950 55.405 222 95 3 37 257 61 279 1.43e-86 259
MS.gene22589.t1 MTR_7g110990 55.217 230 97 4 37 262 12 239 2.05e-86 257
MS.gene22589.t1 MTR_3g094040 56.109 221 95 2 37 257 31 249 3.18e-86 257
MS.gene22589.t1 MTR_4g063975 50.000 250 123 2 11 259 6 254 4.04e-86 257
MS.gene22589.t1 MTR_8g464350 50.000 258 122 5 6 262 3 254 7.28e-86 256
MS.gene22589.t1 MTR_3g435430 53.648 233 103 4 27 257 14 243 8.95e-86 256
MS.gene22589.t1 MTR_5g075320 51.037 241 116 2 16 256 8 246 2.61e-85 255
MS.gene22589.t1 MTR_1g026020 50.000 240 115 4 26 262 18 255 1.38e-83 251
MS.gene22589.t1 MTR_8g058990 53.913 230 100 4 35 262 28 253 2.86e-83 250
MS.gene22589.t1 MTR_7g013140 54.825 228 99 3 36 262 27 251 4.20e-83 249
MS.gene22589.t1 MTR_7g013150 48.638 257 124 4 7 262 4 253 3.50e-81 244
MS.gene22589.t1 MTR_7g013120 52.193 228 104 4 37 262 29 253 6.11e-81 244
MS.gene22589.t1 MTR_7g011060 52.444 225 97 3 35 259 24 238 2.82e-80 242
MS.gene22589.t1 MTR_3g466760 51.639 244 116 2 13 256 14 255 4.05e-80 242
MS.gene22589.t1 MTR_3g466790 51.230 244 117 2 13 256 8 249 2.76e-79 239
MS.gene22589.t1 MTR_4g124340 49.333 225 109 4 37 259 61 282 3.52e-78 238
MS.gene22589.t1 MTR_6g012520 49.231 260 108 5 1 259 4 240 2.55e-70 216
MS.gene22589.t1 MTR_0040s0140 41.935 248 139 3 16 259 8 254 3.20e-68 211
MS.gene22589.t1 MTR_5g076950 45.778 225 121 1 35 259 39 262 2.08e-67 210
MS.gene22589.t1 MTR_2g090255 39.506 243 145 2 18 259 9 250 5.07e-64 201
MS.gene22589.t1 MTR_3g064510 43.777 233 122 5 35 259 51 282 1.53e-60 193
MS.gene22589.t1 MTR_0008s0120 36.576 257 103 4 6 262 3 199 1.13e-50 165
MS.gene22589.t1 MTR_2g090270 31.868 273 150 11 3 262 9 258 1.07e-31 117
MS.gene22589.t1 MTR_0122s0020 33.333 240 132 10 37 263 41 265 1.86e-30 114
MS.gene22589.t1 MTR_2g090265 31.111 270 157 11 6 262 3 256 2.78e-30 114
MS.gene22589.t1 MTR_4g028640 30.877 285 155 12 1 263 1 265 1.51e-27 107
MS.gene22589.t1 MTR_6g012500 48.485 99 50 1 161 259 181 278 4.51e-25 100
MS.gene22589.t1 MTR_0118s0070 29.958 237 136 9 42 262 53 275 1.24e-21 91.7
MS.gene22589.t1 MTR_0809s0010 28.685 251 145 9 28 262 38 270 7.78e-20 86.7
MS.gene22589.t1 MTR_5g013440 25.490 255 157 7 27 263 13 252 4.43e-19 84.3
MS.gene22589.t1 MTR_0122s0020 32.584 178 94 8 37 202 41 204 9.13e-19 82.4
MS.gene22589.t1 MTR_4g075790 28.634 227 131 6 50 262 34 243 1.31e-15 74.7
MS.gene22589.t1 MTR_4g099370 25.652 230 156 5 40 263 31 251 6.70e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22589.t1 AT1G12560 68.826 247 74 2 17 263 19 262 1.96e-126 360
MS.gene22589.t1 AT1G62980 63.320 259 89 2 5 263 5 257 3.25e-123 351
MS.gene22589.t1 AT2G39700 54.582 251 108 3 11 256 1 250 1.73e-95 281
MS.gene22589.t1 AT3G55500 51.807 249 117 3 10 256 6 253 1.02e-92 274
MS.gene22589.t1 AT2G37640 50.973 257 123 3 8 263 7 261 7.84e-91 270
MS.gene22589.t1 AT2G03090 56.951 223 94 2 37 259 30 250 9.41e-89 264
MS.gene22589.t1 AT1G69530 52.227 247 116 2 11 257 1 245 2.15e-88 263
MS.gene22589.t1 AT1G69530 52.227 247 116 2 11 257 1 245 2.15e-88 263
MS.gene22589.t1 AT1G69530 52.227 247 116 2 11 257 1 245 3.06e-88 263
MS.gene22589.t1 AT5G02260 49.219 256 127 3 5 259 1 254 4.29e-88 262
MS.gene22589.t1 AT2G28950 53.043 230 106 2 31 259 25 253 4.63e-87 259
MS.gene22589.t1 AT1G20190 53.138 239 107 3 27 262 16 252 5.81e-86 257
MS.gene22589.t1 AT4G01630 53.478 230 101 4 37 262 28 255 3.18e-85 255
MS.gene22589.t1 AT5G56320 50.400 250 116 4 11 259 7 249 1.22e-84 253
MS.gene22589.t1 AT5G56320 50.400 250 116 4 11 259 7 249 1.22e-84 253
MS.gene22589.t1 AT5G56320 50.400 250 116 4 11 259 7 249 6.20e-84 253
MS.gene22589.t1 AT2G40610 54.955 222 95 4 37 256 30 248 2.52e-83 250
MS.gene22589.t1 AT2G37640 54.976 211 92 3 54 263 28 236 5.40e-82 246
MS.gene22589.t1 AT5G05290 51.351 222 105 3 37 257 32 251 5.23e-80 241
MS.gene22589.t1 AT1G69530 50.855 234 110 3 11 244 1 229 4.49e-79 239
MS.gene22589.t1 AT1G69530 54.286 210 95 1 11 220 1 209 1.38e-78 237
MS.gene22589.t1 AT1G26770 55.204 221 97 2 37 257 26 244 2.32e-78 237
MS.gene22589.t1 AT1G26770 55.204 221 97 2 37 257 36 254 2.97e-78 237
MS.gene22589.t1 AT5G39300 45.387 271 127 6 8 262 11 276 3.16e-78 238
MS.gene22589.t1 AT5G39270 49.378 241 111 5 28 262 44 279 4.36e-78 238
MS.gene22589.t1 AT5G39280 46.442 267 130 6 1 262 17 275 6.07e-78 237
MS.gene22589.t1 AT5G39290 48.963 241 112 5 28 262 44 279 1.45e-76 234
MS.gene22589.t1 AT3G29030 50.901 222 98 4 37 257 38 249 1.99e-74 228
MS.gene22589.t1 AT5G39310 47.845 232 110 6 35 262 88 312 2.67e-73 226
MS.gene22589.t1 AT3G03220 44.643 224 123 1 36 259 39 261 1.05e-67 211
MS.gene22589.t1 AT3G15370 48.246 228 106 6 37 259 27 247 2.96e-66 206
MS.gene22589.t1 AT1G20190 51.031 194 90 3 72 262 2 193 1.49e-65 202
MS.gene22589.t1 AT3G15370 48.815 211 96 6 54 259 74 277 5.63e-60 191
MS.gene22589.t1 AT4G38210 40.948 232 132 4 35 263 26 255 2.14e-59 189
MS.gene22589.t1 AT5G39260 40.377 265 135 8 8 262 11 262 5.71e-57 183
MS.gene22589.t1 AT4G28250 33.058 242 133 8 35 262 34 260 1.20e-33 123
MS.gene22589.t1 AT1G65680 33.865 251 137 9 27 262 37 273 1.01e-32 120
MS.gene22589.t1 AT4G28250 32.645 242 128 8 35 262 34 254 1.73e-31 117
MS.gene22589.t1 AT2G20750 32.231 242 138 9 35 262 38 267 8.44e-31 115
MS.gene22589.t1 AT2G45110 31.343 268 150 8 10 262 11 259 7.87e-28 107
MS.gene22589.t1 AT1G65681 33.784 222 122 8 53 262 14 222 2.87e-25 100
MS.gene22589.t1 AT3G60570 26.592 267 168 7 8 262 7 257 1.20e-21 91.3
MS.gene22589.t1 AT4G28250 31.073 177 90 6 35 199 34 190 4.42e-18 80.5
MS.gene22589.t1 AT4G28250 30.556 180 93 6 35 202 34 193 5.19e-18 80.5
MS.gene22589.t1 AT2G20750 29.944 177 101 7 35 199 38 203 1.42e-17 79.3
MS.gene22589.t1 AT4G17030 23.735 257 179 7 11 260 1 247 6.89e-17 78.2
MS.gene22589.t1 AT3G60570 26.818 220 135 6 55 262 4 209 1.25e-16 76.6
MS.gene22589.t1 AT3G45970 25.463 216 143 7 56 262 15 221 4.99e-12 64.3
MS.gene22589.t1 AT4G38400 26.066 211 139 8 60 262 47 248 1.58e-11 63.5

Find 81 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGTCAAGCTCTTTCTTTC+AGG 0.154213 5.2:-3987775 MS.gene22589:CDS
GCAATGATGTTCACTTTCAT+TGG 0.304434 5.2:-3988671 MS.gene22589:CDS
CCAATCTTTGCCCTCCTAAT+TGG 0.315819 5.2:-3988195 MS.gene22589:CDS
CTTTCCAAGGAAACGGATAC+TGG 0.324679 5.2:-3987928 MS.gene22589:CDS
AGCAGTTTCATCTCCATAAA+AGG 0.326533 5.2:+3988589 None:intergenic
TTGATTGGTAGCATTTAATC+TGG 0.327109 5.2:+3988260 None:intergenic
GGATTAAGATGAGCCATAAC+TGG 0.340784 5.2:-3987829 MS.gene22589:CDS
ATTTACCTGCGGTACATAAC+AGG 0.349515 5.2:+3988067 None:intergenic
TCAGAGGGTTTGGACCAATT+AGG 0.358998 5.2:+3988181 None:intergenic
TCCACCATTATCAGAGGGTT+TGG 0.384127 5.2:+3988171 None:intergenic
TGGTCCAAACCCTCTGATAA+TGG 0.386369 5.2:-3988175 MS.gene22589:CDS
TTCTATAACATGCACTTGAT+TGG 0.400131 5.2:+3988245 None:intergenic
AATTTGTTTCAGAATGGTTA+CGG 0.419394 5.2:-3988346 MS.gene22589:CDS
CGTTTCCTTGGAAAGAGAAT+CGG 0.432181 5.2:+3987936 None:intergenic
ATGAGCAAGAGACCATTTAC+TGG 0.447346 5.2:+3988616 None:intergenic
ATTTGTTTCAGAATGGTTAC+GGG 0.448067 5.2:-3988345 MS.gene22589:CDS
GGAATGTTGCTCCTTCCAAT+TGG 0.448353 5.2:-3987715 MS.gene22589:CDS
GTGATGAACGTCGGAGGTGG+AGG 0.449688 5.2:-3987893 MS.gene22589:CDS
ATGGTGAAAGGAAGCAGAAC+AGG 0.453444 5.2:-3987854 MS.gene22589:CDS
GGTAGGAAGCACCCCAGTTA+TGG 0.453717 5.2:+3987816 None:intergenic
AGTGAGTCCCACATTCCAAT+TGG 0.458274 5.2:+3987700 None:intergenic
CGATTCTCTTTCCAAGGAAA+CGG 0.460365 5.2:-3987935 MS.gene22589:CDS
ATAGCCAGTATCCGTTTCCT+TGG 0.460878 5.2:+3987924 None:intergenic
CCATGCAATAATAGTCTCCT+TGG 0.461696 5.2:+3987736 None:intergenic
TACGGAAATTTGTTTCAGAA+TGG 0.468116 5.2:-3988352 MS.gene22589:CDS
CGATATTTAGACCCAGTAAA+TGG 0.471376 5.2:-3988628 MS.gene22589:CDS
TACGTGATGAACGTCGGAGG+TGG 0.485202 5.2:-3987896 MS.gene22589:CDS
TCAGTGGAAGTTGACCCTAC+TGG 0.496511 5.2:+3987673 None:intergenic
AAAATTGCACAATGGAAAGC+TGG 0.510005 5.2:-3988097 MS.gene22589:CDS
CCAATTAGGAGGGCAAAGAT+TGG 0.512665 5.2:+3988195 None:intergenic
TGCAATAATAGTCTCCTTGG+TGG 0.513313 5.2:+3987739 None:intergenic
GCTCCTTCCAATTGGAATGT+GGG 0.514369 5.2:-3987707 MS.gene22589:CDS
TGAAACTGCTTCTGAAACCA+TGG 0.519577 5.2:-3988576 MS.gene22589:CDS
ATAGGAGGAGCATGTGGATA+CGG 0.528346 5.2:-3988370 MS.gene22589:intron
AGGGTGCCATGCCAAAGAAA+AGG 0.533175 5.2:-3987965 MS.gene22589:CDS
CACCTAGTGTTGCAAATGCT+TGG 0.543954 5.2:+3987795 None:intergenic
TCAACAATGGTTATGCGTGC+GGG 0.548343 5.2:-3988291 MS.gene22589:CDS
TTCAACAATGGTTATGCGTG+CGG 0.553756 5.2:-3988292 MS.gene22589:CDS
TAGTGTTGCAAATGCTTGGT+AGG 0.556322 5.2:+3987799 None:intergenic
GATTAAGATGAGCCATAACT+GGG 0.557111 5.2:-3987828 MS.gene22589:CDS
GGAATCCGATTCTCTTTCCA+AGG 0.558029 5.2:-3987941 MS.gene22589:CDS
GAGGGTTTGGACCAATTAGG+AGG 0.565605 5.2:+3988184 None:intergenic
CACCATCCACCATTATCAGA+GGG 0.568584 5.2:+3988166 None:intergenic
TCGTCCCTGTTATGTACCGC+AGG 0.572591 5.2:-3988072 MS.gene22589:intron
AGCTCGACGTTGTTCAACAA+TGG 0.574107 5.2:-3988304 MS.gene22589:CDS
GAATGGTTACGGGACAGATA+CGG 0.574566 5.2:-3988335 MS.gene22589:CDS
GGTGGCGGTGACAGTGGTGA+AGG 0.578310 5.2:+3988216 None:intergenic
ATTAGGAGGGCAAAGATTGG+TGG 0.578409 5.2:+3988198 None:intergenic
TGGGACTCACTTATTCCAGT+AGG 0.581986 5.2:-3987688 MS.gene22589:CDS
TGCTCCTTCCAATTGGAATG+TGG 0.582320 5.2:-3987708 MS.gene22589:CDS
ATGTCGAGCATGATGGTGAA+AGG 0.595570 5.2:-3987866 MS.gene22589:CDS
AGTCCCACATTCCAATTGGA+AGG 0.604827 5.2:+3987704 None:intergenic
AGGAGGGCAAAGATTGGTGG+CGG 0.613908 5.2:+3988201 None:intergenic
ATGTCAAAATGAACTCGAGG+TGG 0.616294 5.2:+3988139 None:intergenic
GAAACTGCTTCTGAAACCAT+GGG 0.617888 5.2:-3988575 MS.gene22589:intron
GGGACTCACTTATTCCAGTA+GGG 0.618858 5.2:-3987687 MS.gene22589:CDS
CCAAGGAGACTATTATTGCA+TGG 0.618930 5.2:-3987736 MS.gene22589:CDS
TTTACCTGCGGTACATAACA+GGG 0.620314 5.2:+3988068 None:intergenic
AAGTGCATGTTATAGAAATG+TGG 0.620944 5.2:-3988239 MS.gene22589:CDS
AGGAGATATGTCGAGCATGA+TGG 0.627916 5.2:-3987873 MS.gene22589:CDS
ACACCATCCACCATTATCAG+AGG 0.628985 5.2:+3988165 None:intergenic
AGGGTTTGGACCAATTAGGA+GGG 0.631610 5.2:+3988185 None:intergenic
GTGCCATGCCAAAGAAAAGG+AGG 0.634927 5.2:-3987962 MS.gene22589:CDS
TGAAAGGAAGCAGAACAGGA+TGG 0.636895 5.2:-3987850 MS.gene22589:CDS
TGGAGAACAAACAGTAGCTG+TGG 0.637218 5.2:-3988651 MS.gene22589:CDS
TGTCAAAATGAACTCGAGGT+GGG 0.638616 5.2:+3988140 None:intergenic
AAAATTATAGGAGGAGCATG+TGG 0.639946 5.2:-3988376 MS.gene22589:intron
TGAGCAAGAGACCATTTACT+GGG 0.649236 5.2:+3988617 None:intergenic
GCCATGTCAAAATGAACTCG+AGG 0.653200 5.2:+3988136 None:intergenic
CAAGCATTTGCAACACTAGG+TGG 0.653587 5.2:-3987794 MS.gene22589:CDS
AAGATTGGTGGCGGTGACAG+TGG 0.654167 5.2:+3988210 None:intergenic
TACCAAGCATTTGCAACACT+AGG 0.658315 5.2:-3987797 MS.gene22589:CDS
TTAGTCTACGTGATGAACGT+CGG 0.658332 5.2:-3987902 MS.gene22589:CDS
AGTTTCATCTCCATAAAAGG+TGG 0.665867 5.2:+3988592 None:intergenic
AACCCTCTGATAATGGTGGA+TGG 0.666609 5.2:-3988168 MS.gene22589:CDS
CCTTCATGAAAATTGCACAA+TGG 0.672430 5.2:-3988105 MS.gene22589:CDS
GTCTACGTGATGAACGTCGG+AGG 0.675538 5.2:-3987899 MS.gene22589:CDS
TCCAAACCCTCTGATAATGG+TGG 0.691504 5.2:-3988172 MS.gene22589:CDS
TGGTTACGGGACAGATACGG+TGG 0.722045 5.2:-3988332 MS.gene22589:CDS
GCTTACTTCATACACCACCA+AGG 0.752925 5.2:-3987753 MS.gene22589:CDS
ATTAAGATGAGCCATAACTG+GGG 0.796701 5.2:-3987827 MS.gene22589:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCTGATTATTTCATACTA+AGG + chr5.2:3988370-3988389 None:intergenic 20.0%
!! TAAATGTATATATATACCCA+TGG + chr5.2:3987833-3987852 None:intergenic 20.0%
!!! TTTTTTTGTTAATATGGTTG+TGG - chr5.2:3987988-3988007 MS.gene22589:intron 20.0%
!! AATTTGTTTCAGAATGGTTA+CGG - chr5.2:3988043-3988062 MS.gene22589:intron 25.0%
!! TGCTTGTTGATTAATTTACA+GGG - chr5.2:3988405-3988424 MS.gene22589:intron 25.0%
AAGTGCATGTTATAGAAATG+TGG - chr5.2:3988150-3988169 MS.gene22589:CDS 30.0%
ATCAACAAGCACATTAGTTA+AGG + chr5.2:3988396-3988415 None:intergenic 30.0%
ATGGTTGTGGCTAAAATTAT+AGG - chr5.2:3988001-3988020 MS.gene22589:intron 30.0%
ATTTGTTTCAGAATGGTTAC+GGG - chr5.2:3988044-3988063 MS.gene22589:intron 30.0%
TCAACAAGCACATTAGTTAA+GGG + chr5.2:3988395-3988414 None:intergenic 30.0%
TTCTATAACATGCACTTGAT+TGG + chr5.2:3988147-3988166 None:intergenic 30.0%
! GTGCTTGTTGATTAATTTAC+AGG - chr5.2:3988404-3988423 MS.gene22589:intron 30.0%
! TACGGAAATTTGTTTCAGAA+TGG - chr5.2:3988037-3988056 MS.gene22589:intron 30.0%
! TTGATTGGTAGCATTTAATC+TGG + chr5.2:3988132-3988151 None:intergenic 30.0%
AAAATTGCACAATGGAAAGC+TGG - chr5.2:3988292-3988311 MS.gene22589:CDS 35.0%
AGCAGTTTCATCTCCATAAA+AGG + chr5.2:3987803-3987822 None:intergenic 35.0%
AGTTTCATCTCCATAAAAGG+TGG + chr5.2:3987800-3987819 None:intergenic 35.0%
ATTAAGATGAGCCATAACTG+GGG - chr5.2:3988562-3988581 MS.gene22589:intron 35.0%
CCTTCATGAAAATTGCACAA+TGG - chr5.2:3988284-3988303 MS.gene22589:CDS 35.0%
CGATATTTAGACCCAGTAAA+TGG - chr5.2:3987761-3987780 MS.gene22589:CDS 35.0%
GATTAAGATGAGCCATAACT+GGG - chr5.2:3988561-3988580 MS.gene22589:intron 35.0%
GCAATGATGTTCACTTTCAT+TGG - chr5.2:3987718-3987737 MS.gene22589:CDS 35.0%
GTTGTGGCTAAAATTATAGG+AGG - chr5.2:3988004-3988023 MS.gene22589:intron 35.0%
! AAAATTATAGGAGGAGCATG+TGG - chr5.2:3988013-3988032 MS.gene22589:intron 35.0%
! AATTTTCATGAAGGCAGGTT+TGG + chr5.2:3988278-3988297 None:intergenic 35.0%
! CCATTGTGCAATTTTCATGA+AGG + chr5.2:3988287-3988306 None:intergenic 35.0%
ATGAGCAAGAGACCATTTAC+TGG + chr5.2:3987776-3987795 None:intergenic 40.0%
ATGTCAAAATGAACTCGAGG+TGG + chr5.2:3988253-3988272 None:intergenic 40.0%
ATTTACCTGCGGTACATAAC+AGG + chr5.2:3988325-3988344 None:intergenic 40.0%
CCAAGGAGACTATTATTGCA+TGG - chr5.2:3988653-3988672 MS.gene22589:CDS 40.0%
CCATGCAATAATAGTCTCCT+TGG + chr5.2:3988656-3988675 None:intergenic 40.0%
CGATTCTCTTTCCAAGGAAA+CGG - chr5.2:3988454-3988473 MS.gene22589:intron 40.0%
CGTTTCCTTGGAAAGAGAAT+CGG + chr5.2:3988456-3988475 None:intergenic 40.0%
GGATTAAGATGAGCCATAAC+TGG - chr5.2:3988560-3988579 MS.gene22589:intron 40.0%
TACCAAGCATTTGCAACACT+AGG - chr5.2:3988592-3988611 MS.gene22589:CDS 40.0%
TAGTGTTGCAAATGCTTGGT+AGG + chr5.2:3988593-3988612 None:intergenic 40.0%
TGAGCAAGAGACCATTTACT+GGG + chr5.2:3987775-3987794 None:intergenic 40.0%
TGCAATAATAGTCTCCTTGG+TGG + chr5.2:3988653-3988672 None:intergenic 40.0%
TGTCAAAATGAACTCGAGGT+GGG + chr5.2:3988252-3988271 None:intergenic 40.0%
TTAGTCTACGTGATGAACGT+CGG - chr5.2:3988487-3988506 MS.gene22589:intron 40.0%
TTCAACAATGGTTATGCGTG+CGG - chr5.2:3988097-3988116 MS.gene22589:CDS 40.0%
TTTACCTGCGGTACATAACA+GGG + chr5.2:3988324-3988343 None:intergenic 40.0%
! ATCGGATTCCTCCTTTTCTT+TGG + chr5.2:3988438-3988457 None:intergenic 40.0%
! ATTCCTCCTTTTCTTTGGCA+TGG + chr5.2:3988433-3988452 None:intergenic 40.0%
! TGTGCAATTTTCATGAAGGC+AGG + chr5.2:3988283-3988302 None:intergenic 40.0%
!! ACCTCGAGTTCATTTTGACA+TGG - chr5.2:3988252-3988271 MS.gene22589:CDS 40.0%
!! GAAACTGCTTCTGAAACCAT+GGG - chr5.2:3987814-3987833 MS.gene22589:CDS 40.0%
!! TGAAACTGCTTCTGAAACCA+TGG - chr5.2:3987813-3987832 MS.gene22589:CDS 40.0%
ACACCATCCACCATTATCAG+AGG + chr5.2:3988227-3988246 None:intergenic 45.0%
AGCTCGACGTTGTTCAACAA+TGG - chr5.2:3988085-3988104 MS.gene22589:CDS 45.0%
AGGAGATATGTCGAGCATGA+TGG - chr5.2:3988516-3988535 MS.gene22589:intron 45.0%
AGGGTTTGGACCAATTAGGA+GGG + chr5.2:3988207-3988226 None:intergenic 45.0%
AGTCCCACATTCCAATTGGA+AGG + chr5.2:3988688-3988707 None:intergenic 45.0%
AGTGAGTCCCACATTCCAAT+TGG + chr5.2:3988692-3988711 None:intergenic 45.0%
ATAGCCAGTATCCGTTTCCT+TGG + chr5.2:3988468-3988487 None:intergenic 45.0%
ATGGTGAAAGGAAGCAGAAC+AGG - chr5.2:3988535-3988554 MS.gene22589:intron 45.0%
ATTAGGAGGGCAAAGATTGG+TGG + chr5.2:3988194-3988213 None:intergenic 45.0%
CAAGCATTTGCAACACTAGG+TGG - chr5.2:3988595-3988614 MS.gene22589:CDS 45.0%
CACCATCCACCATTATCAGA+GGG + chr5.2:3988226-3988245 None:intergenic 45.0%
CACCTAGTGTTGCAAATGCT+TGG + chr5.2:3988597-3988616 None:intergenic 45.0%
CCAATCTTTGCCCTCCTAAT+TGG - chr5.2:3988194-3988213 MS.gene22589:CDS 45.0%
CCAATTAGGAGGGCAAAGAT+TGG + chr5.2:3988197-3988216 None:intergenic 45.0%
CTTTCCAAGGAAACGGATAC+TGG - chr5.2:3988461-3988480 MS.gene22589:intron 45.0%
GAATGGTTACGGGACAGATA+CGG - chr5.2:3988054-3988073 MS.gene22589:intron 45.0%
GCCATGTCAAAATGAACTCG+AGG + chr5.2:3988256-3988275 None:intergenic 45.0%
GCGCATTGTGAATTTACCTG+CGG + chr5.2:3988336-3988355 None:intergenic 45.0%
GCTCCTTCCAATTGGAATGT+GGG - chr5.2:3988682-3988701 MS.gene22589:CDS 45.0%
GCTTACTTCATACACCACCA+AGG - chr5.2:3988636-3988655 MS.gene22589:CDS 45.0%
GGAATCCGATTCTCTTTCCA+AGG - chr5.2:3988448-3988467 MS.gene22589:intron 45.0%
GGAATGTTGCTCCTTCCAAT+TGG - chr5.2:3988674-3988693 MS.gene22589:CDS 45.0%
GGGACTCACTTATTCCAGTA+GGG - chr5.2:3988702-3988721 MS.gene22589:CDS 45.0%
GTGGTCAAGCTCTTTCTTTC+AGG - chr5.2:3988614-3988633 MS.gene22589:CDS 45.0%
TCAACAATGGTTATGCGTGC+GGG - chr5.2:3988098-3988117 MS.gene22589:CDS 45.0%
TCAGAGGGTTTGGACCAATT+AGG + chr5.2:3988211-3988230 None:intergenic 45.0%
TCCACCATTATCAGAGGGTT+TGG + chr5.2:3988221-3988240 None:intergenic 45.0%
TGAAAGGAAGCAGAACAGGA+TGG - chr5.2:3988539-3988558 MS.gene22589:intron 45.0%
TGCTCCTTCCAATTGGAATG+TGG - chr5.2:3988681-3988700 MS.gene22589:CDS 45.0%
TGGAGAACAAACAGTAGCTG+TGG - chr5.2:3987738-3987757 MS.gene22589:CDS 45.0%
TGGGACTCACTTATTCCAGT+AGG - chr5.2:3988701-3988720 MS.gene22589:CDS 45.0%
TGGTCCAAACCCTCTGATAA+TGG - chr5.2:3988214-3988233 MS.gene22589:CDS 45.0%
! ATAGGAGGAGCATGTGGATA+CGG - chr5.2:3988019-3988038 MS.gene22589:intron 45.0%
! ATGTCGAGCATGATGGTGAA+AGG - chr5.2:3988523-3988542 MS.gene22589:intron 45.0%
! CTTGCTCATGCCACCTTTTA+TGG - chr5.2:3987787-3987806 MS.gene22589:CDS 45.0%
!! AACCCTCTGATAATGGTGGA+TGG - chr5.2:3988221-3988240 MS.gene22589:CDS 45.0%
!! TCCAAACCCTCTGATAATGG+TGG - chr5.2:3988217-3988236 MS.gene22589:CDS 45.0%
!!! ATATATTTTTTTTGTTAATA+TGG - chr5.2:3987982-3988001 MS.gene22589:intron 5.0%
GAGGGTTTGGACCAATTAGG+AGG + chr5.2:3988208-3988227 None:intergenic 50.0%
GTGCCATGCCAAAGAAAAGG+AGG - chr5.2:3988427-3988446 MS.gene22589:intron 50.0%
TCAGTGGAAGTTGACCCTAC+TGG + chr5.2:3988719-3988738 None:intergenic 50.0%
! AGGGTGCCATGCCAAAGAAA+AGG - chr5.2:3988424-3988443 MS.gene22589:intron 50.0%
AGGAGGGCAAAGATTGGTGG+CGG + chr5.2:3988191-3988210 None:intergenic 55.0%
GTCTACGTGATGAACGTCGG+AGG - chr5.2:3988490-3988509 MS.gene22589:intron 55.0%
TACGTGATGAACGTCGGAGG+TGG - chr5.2:3988493-3988512 MS.gene22589:intron 55.0%
TCGTCCCTGTTATGTACCGC+AGG - chr5.2:3988317-3988336 MS.gene22589:CDS 55.0%
TGGTTACGGGACAGATACGG+TGG - chr5.2:3988057-3988076 MS.gene22589:intron 55.0%
!! AAGATTGGTGGCGGTGACAG+TGG + chr5.2:3988182-3988201 None:intergenic 55.0%
!! GGTAGGAAGCACCCCAGTTA+TGG + chr5.2:3988576-3988595 None:intergenic 55.0%
GTGATGAACGTCGGAGGTGG+AGG - chr5.2:3988496-3988515 MS.gene22589:intron 60.0%
! GGTGGCGGTGACAGTGGTGA+AGG + chr5.2:3988176-3988195 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 3987673 3988738 3987673 ID=MS.gene22589
chr5.2 mRNA 3987673 3988738 3987673 ID=MS.gene22589.t1;Parent=MS.gene22589
chr5.2 exon 3988576 3988738 3988576 ID=MS.gene22589.t1.exon1;Parent=MS.gene22589.t1
chr5.2 CDS 3988576 3988738 3988576 ID=cds.MS.gene22589.t1;Parent=MS.gene22589.t1
chr5.2 exon 3988073 3988388 3988073 ID=MS.gene22589.t1.exon2;Parent=MS.gene22589.t1
chr5.2 CDS 3988073 3988388 3988073 ID=cds.MS.gene22589.t1;Parent=MS.gene22589.t1
chr5.2 exon 3987673 3987985 3987673 ID=MS.gene22589.t1.exon3;Parent=MS.gene22589.t1
chr5.2 CDS 3987673 3987985 3987673 ID=cds.MS.gene22589.t1;Parent=MS.gene22589.t1
Gene Sequence

>MS.gene22589

ATGGCATTTATTCTTCAATCTCGTTGCTTCATGTTGATTGCTTATGCAATGATGTTCACTTTCATTGGAGAACAAACAGTAGCTGTGGCGATATTTAGACCCAGTAAATGGTCTCTTGCTCATGCCACCTTTTATGGAGATGAAACTGCTTCTGAAACCATGGGTATATATATACATTTATATGTTATCTCAATTATCATTACTTCTCACTTAGCTTAATTAAGTTCAGCATTATTAGTATCATTCAACATCTCACAACTTCAATTTATTAATTAAATTAAACTACTTTTACTTAGTATCAATATTGATATATATTTTTTTTGTTAATATGGTTGTGGCTAAAATTATAGGAGGAGCATGTGGATACGGAAATTTGTTTCAGAATGGTTACGGGACAGATACGGTGGCTTTAAGCTCGACGTTGTTCAACAATGGTTATGCGTGCGGGACATGTTTCCAGATTAAATGCTACCAATCAAGTGCATGTTATAGAAATGTGGCCTTCACCACTGTCACCGCCACCAATCTTTGCCCTCCTAATTGGTCCAAACCCTCTGATAATGGTGGATGGTGTAACCCACCTCGAGTTCATTTTGACATGGCCAAACCTGCCTTCATGAAAATTGCACAATGGAAAGCTGGTATCGTCCCTGTTATGTACCGCAGGTAAATTCACAATGCGCATTTTAACTTTCCTTAGTATGAAATAATCAGATTTTCCCTTAACTAATGTGCTTGTTGATTAATTTACAGGGTGCCATGCCAAAGAAAAGGAGGAATCCGATTCTCTTTCCAAGGAAACGGATACTGGCTATTAGTCTACGTGATGAACGTCGGAGGTGGAGGAGATATGTCGAGCATGATGGTGAAAGGAAGCAGAACAGGATGGATTAAGATGAGCCATAACTGGGGTGCTTCCTACCAAGCATTTGCAACACTAGGTGGTCAAGCTCTTTCTTTCAGGCTTACTTCATACACCACCAAGGAGACTATTATTGCATGGAATGTTGCTCCTTCCAATTGGAATGTGGGACTCACTTATTCCAGTAGGGTCAACTTCCACTGA
Protein sequence

>MS.gene22589.t1

MAFILQSRCFMLIAYAMMFTFIGEQTVAVAIFRPSKWSLAHATFYGDETASETMGGACGYGNLFQNGYGTDTVALSSTLFNNGYACGTCFQIKCYQSSACYRNVAFTTVTATNLCPPNWSKPSDNGGWCNPPRVHFDMAKPAFMKIAQWKAGIVPVMYRRVPCQRKGGIRFSFQGNGYWLLVYVMNVGGGGDMSSMMVKGSRTGWIKMSHNWGASYQAFATLGGQALSFRLTSYTTKETIIAWNVAPSNWNVGLTYSSRVNFH