AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37224

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37224.t1 MTR_4g102450 96.341 246 9 0 1 246 17 262 5.92e-179 492
MS.gene37224.t1 MTR_5g011210 80.894 246 47 0 1 246 18 263 2.04e-153 427
MS.gene37224.t1 MTR_5g079950 54.065 246 110 3 2 246 15 258 1.81e-93 275
MS.gene37224.t1 MTR_2g097030 51.667 240 114 2 2 240 16 254 4.17e-90 267
MS.gene37224.t1 MTR_7g061720 52.893 242 111 3 6 246 18 257 9.76e-88 261
MS.gene37224.t1 MTR_1g085460 53.219 233 105 3 16 245 26 257 6.52e-86 256
MS.gene37224.t1 MTR_4g063975 52.720 239 110 3 9 246 21 257 5.12e-85 254
MS.gene37224.t1 MTR_7g111010 51.210 248 117 3 1 245 8 254 5.29e-85 253
MS.gene37224.t1 MTR_5g041700 55.856 222 94 3 20 240 25 243 5.53e-85 253
MS.gene37224.t1 MTR_3g094040 55.405 222 95 3 20 240 31 249 1.10e-84 253
MS.gene37224.t1 MTR_7g110990 55.022 229 99 3 20 245 12 239 1.15e-84 252
MS.gene37224.t1 MTR_3g094060 54.955 222 96 3 20 240 31 249 3.46e-84 251
MS.gene37224.t1 MTR_4g081950 55.405 222 95 3 20 240 61 279 3.93e-84 252
MS.gene37224.t1 MTR_3g435430 54.955 222 96 3 20 240 25 243 2.31e-83 249
MS.gene37224.t1 MTR_5g075320 53.846 221 98 3 20 239 29 246 1.28e-81 245
MS.gene37224.t1 MTR_8g058990 53.043 230 102 4 18 245 28 253 1.38e-81 245
MS.gene37224.t1 MTR_7g011060 51.452 241 105 4 1 240 7 236 2.11e-80 241
MS.gene37224.t1 MTR_1g026020 50.215 233 113 2 15 245 24 255 5.99e-80 241
MS.gene37224.t1 MTR_8g464350 51.528 229 107 3 18 245 29 254 1.00e-79 240
MS.gene37224.t1 MTR_4g124340 48.319 238 118 4 4 239 45 279 1.43e-79 241
MS.gene37224.t1 MTR_7g013140 53.070 228 103 3 19 245 27 251 1.69e-79 239
MS.gene37224.t1 MTR_3g466760 54.054 222 98 3 20 240 38 256 8.34e-79 238
MS.gene37224.t1 MTR_7g013120 52.402 229 104 4 19 245 28 253 1.06e-78 238
MS.gene37224.t1 MTR_3g466790 54.299 221 97 3 20 239 32 249 2.31e-78 237
MS.gene37224.t1 MTR_7g013150 50.877 228 108 3 19 245 29 253 2.19e-76 231
MS.gene37224.t1 MTR_0040s0140 45.815 227 121 2 15 240 27 252 6.86e-69 213
MS.gene37224.t1 MTR_6g012520 52.101 238 102 4 4 240 12 238 1.45e-68 211
MS.gene37224.t1 MTR_2g090255 40.664 241 141 2 1 240 9 248 8.00e-66 205
MS.gene37224.t1 MTR_5g076950 46.667 225 115 3 18 240 39 260 6.75e-65 203
MS.gene37224.t1 MTR_3g064510 45.148 237 109 7 18 240 51 280 2.94e-60 192
MS.gene37224.t1 MTR_0008s0120 33.772 228 93 4 18 245 30 199 3.26e-32 117
MS.gene37224.t1 MTR_2g090270 32.365 241 135 9 18 245 33 258 7.00e-32 117
MS.gene37224.t1 MTR_0122s0020 32.917 240 133 9 20 246 41 265 4.73e-31 115
MS.gene37224.t1 MTR_2g090265 31.557 244 138 10 15 245 29 256 1.73e-29 111
MS.gene37224.t1 MTR_4g028640 33.750 240 130 9 20 246 42 265 1.13e-26 104
MS.gene37224.t1 MTR_6g012500 45.361 97 52 1 144 240 181 276 7.64e-22 91.7
MS.gene37224.t1 MTR_0809s0010 29.150 247 141 9 15 245 42 270 1.71e-21 90.5
MS.gene37224.t1 MTR_0118s0070 29.237 236 139 8 25 245 53 275 2.46e-21 90.5
MS.gene37224.t1 MTR_5g013440 26.271 236 155 7 18 245 27 251 1.80e-20 87.4
MS.gene37224.t1 MTR_0122s0020 33.146 178 93 7 20 185 41 204 2.14e-20 86.3
MS.gene37224.t1 MTR_4g099370 28.980 245 139 9 18 246 26 251 1.63e-18 82.0
MS.gene37224.t1 MTR_4g075790 28.194 227 132 7 33 245 34 243 3.33e-16 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37224.t1 AT1G12560 69.198 237 72 1 10 246 27 262 8.86e-123 350
MS.gene37224.t1 AT1G62980 65.041 246 86 0 1 246 12 257 1.33e-119 342
MS.gene37224.t1 AT3G55500 56.466 232 99 2 9 239 23 253 8.56e-93 274
MS.gene37224.t1 AT2G39700 55.172 232 102 2 9 239 20 250 1.66e-92 273
MS.gene37224.t1 AT2G37640 53.361 238 109 2 4 240 20 256 2.59e-88 262
MS.gene37224.t1 AT5G02260 51.452 241 115 2 1 240 13 252 4.22e-88 261
MS.gene37224.t1 AT4G01630 54.701 234 92 4 20 245 28 255 1.12e-87 260
MS.gene37224.t1 AT2G28950 51.452 241 114 3 1 239 11 250 2.03e-86 257
MS.gene37224.t1 AT2G03090 55.605 223 97 2 18 240 28 248 6.05e-85 253
MS.gene37224.t1 AT1G69530 54.911 224 97 3 20 242 27 247 1.93e-84 253
MS.gene37224.t1 AT1G69530 55.405 222 95 3 20 240 27 245 2.17e-84 252
MS.gene37224.t1 AT1G69530 55.405 222 95 3 20 240 27 245 2.17e-84 252
MS.gene37224.t1 AT5G56320 54.464 224 98 3 18 240 27 247 1.02e-83 250
MS.gene37224.t1 AT5G56320 54.464 224 98 3 18 240 27 247 1.02e-83 250
MS.gene37224.t1 AT5G56320 54.464 224 98 3 18 240 27 247 3.61e-83 251
MS.gene37224.t1 AT2G40610 51.037 241 113 4 1 239 11 248 4.35e-82 246
MS.gene37224.t1 AT1G20190 50.000 238 116 2 10 245 16 252 6.35e-80 241
MS.gene37224.t1 AT2G37640 54.634 205 91 2 37 240 28 231 6.94e-79 238
MS.gene37224.t1 AT5G05290 51.339 224 102 5 20 240 32 251 7.58e-78 235
MS.gene37224.t1 AT5G39300 48.413 252 117 6 1 245 31 276 3.89e-77 234
MS.gene37224.t1 AT5G39280 48.000 250 120 5 1 245 31 275 8.08e-77 233
MS.gene37224.t1 AT5G39270 49.782 229 109 4 18 245 56 279 3.00e-76 232
MS.gene37224.t1 AT1G26770 54.054 222 98 3 20 240 26 244 4.48e-76 231
MS.gene37224.t1 AT1G26770 54.054 222 98 3 20 240 36 254 4.90e-76 231
MS.gene37224.t1 AT1G69530 54.327 208 88 4 20 226 27 228 1.24e-75 229
MS.gene37224.t1 AT5G39290 50.000 228 108 4 19 245 57 279 6.74e-75 229
MS.gene37224.t1 AT3G29030 50.679 221 100 2 20 240 38 249 1.24e-74 227
MS.gene37224.t1 AT1G69530 56.995 193 78 3 20 211 27 215 4.93e-74 224
MS.gene37224.t1 AT5G39310 45.570 237 118 6 13 245 83 312 4.95e-68 213
MS.gene37224.t1 AT3G03220 44.643 224 119 3 19 240 39 259 1.17e-63 200
MS.gene37224.t1 AT3G15370 44.715 246 126 5 1 242 8 247 1.83e-63 199
MS.gene37224.t1 AT4G38210 42.060 233 130 4 17 246 25 255 4.26e-61 193
MS.gene37224.t1 AT1G20190 48.187 193 97 2 55 245 2 193 5.17e-61 191
MS.gene37224.t1 AT3G15370 39.855 276 126 6 1 242 8 277 8.87e-57 182
MS.gene37224.t1 AT5G39260 42.241 232 118 6 18 245 43 262 1.44e-54 176
MS.gene37224.t1 AT4G28250 33.058 242 133 8 18 245 34 260 1.66e-34 125
MS.gene37224.t1 AT4G28250 32.353 238 134 7 18 245 34 254 3.88e-33 121
MS.gene37224.t1 AT1G65680 32.800 250 141 8 10 245 37 273 6.76e-33 120
MS.gene37224.t1 AT2G20750 31.818 242 139 8 18 245 38 267 5.95e-32 118
MS.gene37224.t1 AT2G45110 32.489 237 133 9 23 245 36 259 6.41e-27 104
MS.gene37224.t1 AT1G65681 31.556 225 129 7 34 246 12 223 5.82e-25 99.0
MS.gene37224.t1 AT4G28250 30.682 176 98 5 18 185 34 193 3.97e-20 85.9
MS.gene37224.t1 AT4G28250 31.214 173 95 5 18 182 34 190 4.14e-20 85.5
MS.gene37224.t1 AT2G20750 30.319 188 107 7 18 193 38 213 1.27e-19 84.7
MS.gene37224.t1 AT3G60570 27.386 241 147 7 18 245 32 257 7.97e-19 83.2
MS.gene37224.t1 AT4G17030 27.511 229 147 7 23 243 30 247 1.37e-18 82.4
MS.gene37224.t1 AT3G60570 26.697 221 134 7 38 245 4 209 1.03e-14 71.2
MS.gene37224.t1 AT3G45970 25.806 217 141 6 39 245 15 221 2.81e-12 64.7

Find 78 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCAACATTGTTCAATAA+TGG 0.212841 8.3:-19247545 MS.gene37224:CDS
CCAAGCTATTATAGTTTCTT+TGG 0.229810 8.3:+19246315 None:intergenic
AGGAAATGGGTATTGGTTAT+TGG 0.261742 8.3:-19246500 MS.gene37224:CDS
CAAATATTTGCCCTCCTAAT+TGG 0.264760 8.3:-19247436 MS.gene37224:CDS
CGATTCTCTTTCCAAGGAAA+TGG 0.277433 8.3:-19246514 MS.gene37224:CDS
GGAATGTTGCTCCTTCCAAC+TGG 0.286104 8.3:-19246294 MS.gene37224:CDS
GCTCCTTCCAACTGGGCTGC+TGG 0.323833 8.3:-19246286 MS.gene37224:CDS
ATAGCAAACATGTGGGTTAA+AGG 0.332124 8.3:-19246445 MS.gene37224:CDS
GTTCAACATTGTTCAATAAT+GGG 0.335116 8.3:-19247544 MS.gene37224:CDS
AATTTGTTTCTGAATGGTTA+TGG 0.357643 8.3:-19247587 MS.gene37224:CDS
AATTACCTGCGATACATAAC+AGG 0.358374 8.3:+19247308 None:intergenic
TAATCTTGAAAGAAAGAGTT+TGG 0.362500 8.3:+19246350 None:intergenic
GCACATTTCAACCCAGTTCA+TGG 0.362883 8.3:-19248283 MS.gene37224:CDS
CTTTCCAAGGAAATGGGTAT+TGG 0.363273 8.3:-19246507 MS.gene37224:CDS
ATTTGTTTCTGAATGGTTAT+GGG 0.373242 8.3:-19247586 MS.gene37224:CDS
AAAATTGACATGTGTGGAAT+AGG 0.412567 8.3:+19246258 None:intergenic
GGATTAGCATGAGCCACAAT+TGG 0.416127 8.3:-19246408 MS.gene37224:CDS
TCGGATGCCTGAGACCAATT+AGG 0.422103 8.3:+19247422 None:intergenic
TGGTCTCAGGCATCCGATTA+CGG 0.441938 8.3:-19247416 MS.gene37224:CDS
ATAACCAATACCCATTTCCT+TGG 0.444503 8.3:+19246503 None:intergenic
GATTAGCATGAGCCACAATT+GGG 0.448115 8.3:-19246407 MS.gene37224:CDS
TGGGTTAAAGGAAGTAGAAC+AGG 0.452727 8.3:-19246433 MS.gene37224:CDS
GATTCTCTTTCCAAGGAAAT+GGG 0.454324 8.3:-19246513 MS.gene37224:CDS
TTGCCCTCCTAATTGGTCTC+AGG 0.458583 8.3:-19247429 MS.gene37224:CDS
GTTGCACCATCCACCGTAAT+CGG 0.465140 8.3:+19247403 None:intergenic
TACCAAGCATTTGCAACACT+TGG 0.478453 8.3:-19246376 MS.gene37224:CDS
TATGGAAATTTGTTTCTGAA+TGG 0.491875 8.3:-19247593 MS.gene37224:CDS
CAAGCATTTGCAACACTTGG+TGG 0.494165 8.3:-19246373 MS.gene37224:CDS
AAAGGAGGAGCATGTGGGTA+TGG 0.494882 8.3:-19247611 MS.gene37224:intron
ATTAGGAGGGCAAATATTTG+TGG 0.499330 8.3:+19247439 None:intergenic
GCAAAGCCAGCTTTAGTAGC+GGG 0.501290 8.3:-19248305 MS.gene37224:CDS
GAATGTTGCTCCTTCCAACT+GGG 0.505072 8.3:-19246293 MS.gene37224:CDS
ATGTGCAAGAGTCCATGAAC+TGG 0.507085 8.3:+19248271 None:intergenic
AATGTGCCCGCTACTAAAGC+TGG 0.511601 8.3:+19248299 None:intergenic
GTGATGAATGTGGGAGGTGG+AGG 0.515112 8.3:-19246472 MS.gene37224:CDS
TACGTGATGAATGTGGGAGG+TGG 0.521955 8.3:-19246475 MS.gene37224:CDS
TGCAAAGCCAGCTTTAGTAG+CGG 0.523369 8.3:-19248306 MS.gene37224:CDS
CTTATTAAAGGAGGAGCATG+TGG 0.525297 8.3:-19247617 MS.gene37224:intron
GGATTTCGATTCTCTTTCCA+AGG 0.530783 8.3:-19246520 MS.gene37224:CDS
CCAAAGAAACTATAATAGCT+TGG 0.533024 8.3:-19246315 MS.gene37224:CDS
CACCAAGTGTTGCAAATGCT+TGG 0.541324 8.3:+19246374 None:intergenic
AAAATTGCACAGTGGAAGGC+CGG 0.541507 8.3:-19247338 MS.gene37224:CDS
GGTTAATCCAGCAGCCCAGT+TGG 0.542121 8.3:+19246279 None:intergenic
ATACATAACAGGGACGATAC+CGG 0.542921 8.3:+19247319 None:intergenic
GACTGTAGTGTAAGGAACAT+TGG 0.545707 8.3:+19247466 None:intergenic
CATGAAAATTGCACAGTGGA+AGG 0.550705 8.3:-19247342 MS.gene37224:CDS
GGTAAGAAGCACCCCAATTG+TGG 0.559335 8.3:+19246395 None:intergenic
ATTGGTGTACGTGATGAATG+TGG 0.559419 8.3:-19246482 MS.gene37224:CDS
TTCAATAATGGGTATGCATG+TGG 0.563755 8.3:-19247533 MS.gene37224:CDS
AATCCAGCAGCCCAGTTGGA+AGG 0.563994 8.3:+19246283 None:intergenic
AGGCATCCGATTACGGTGGA+TGG 0.564250 8.3:-19247409 MS.gene37224:CDS
TCGTCCCTGTTATGTATCGC+AGG 0.570292 8.3:-19247313 MS.gene37224:intron
TTGGTGTACGTGATGAATGT+GGG 0.577309 8.3:-19246481 MS.gene37224:CDS
TTCTTAGAGTGCCTTGCATG+AGG 0.577880 8.3:-19246549 MS.gene37224:intron
GATGCCTGAGACCAATTAGG+AGG 0.590186 8.3:+19247425 None:intergenic
GTGCCTTGCATGAGGAGTGA+AGG 0.590760 8.3:-19246541 MS.gene37224:CDS
GAGACAGCTTCTGCCACCAT+GGG 0.594645 8.3:-19248230 MS.gene37224:intron
TGAGACAGCTTCTGCCACCA+TGG 0.614492 8.3:-19248231 MS.gene37224:CDS
GAGGTGATATAGCAAACATG+TGG 0.614514 8.3:-19246453 MS.gene37224:CDS
TTAAAGGAAGTAGAACAGGA+TGG 0.614528 8.3:-19246429 MS.gene37224:CDS
AATCCTTCACTCCTCATGCA+AGG 0.617867 8.3:+19246538 None:intergenic
TCAATAATGGGTATGCATGT+GGG 0.626106 8.3:-19247532 MS.gene37224:CDS
TGTCTCATCGCCATAAAATG+TGG 0.626462 8.3:+19248247 None:intergenic
GTGGCAGTGACTGTAGTGTA+AGG 0.632028 8.3:+19247458 None:intergenic
ATGCCTGAGACCAATTAGGA+GGG 0.649082 8.3:+19247426 None:intergenic
TTATTAAAGGAGGAGCATGT+GGG 0.672893 8.3:-19247616 MS.gene37224:intron
TGTGCAAGAGTCCATGAACT+GGG 0.678559 8.3:+19248272 None:intergenic
AGGTGATATAGCAAACATGT+GGG 0.681494 8.3:-19246452 MS.gene37224:CDS
ATTAGCATGAGCCACAATTG+GGG 0.689678 8.3:-19246406 MS.gene37224:CDS
TCAATGAAAATTGACATGTG+TGG 0.690286 8.3:+19246252 None:intergenic
GTGTACGTGATGAATGTGGG+AGG 0.693958 8.3:-19246478 MS.gene37224:CDS
TCTCAGGCATCCGATTACGG+TGG 0.700337 8.3:-19247413 MS.gene37224:CDS
GAGATATAACTTACCCATGG+TGG 0.711843 8.3:+19248217 None:intergenic
TGTCAAAATGTGAACGTGGT+GGG 0.713445 8.3:+19247381 None:intergenic
ATGTCAAAATGTGAACGTGG+TGG 0.718706 8.3:+19247380 None:intergenic
ATTACCTGCGATACATAACA+GGG 0.735393 8.3:+19247309 None:intergenic
CCTTCATGAAAATTGCACAG+TGG 0.762866 8.3:-19247346 MS.gene37224:CDS
GACATGTCAAAATGTGAACG+TGG 0.763719 8.3:+19247377 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATATAGAAATAATATAT+TGG + chr8.3:19246852-19246871 None:intergenic 10.0%
!! TATTCTATATTATATATATG+TGG + chr8.3:19247307-19247326 None:intergenic 10.0%
!! TCTAGATATAAATAATTAAA+GGG - chr8.3:19246709-19246728 MS.gene37224:intron 10.0%
!! TTAATTATTTATATCTAGAA+GGG + chr8.3:19246708-19246727 None:intergenic 10.0%
!! TTCTAGATATAAATAATTAA+AGG - chr8.3:19246708-19246727 MS.gene37224:intron 10.0%
!! TTTAATTATTTATATCTAGA+AGG + chr8.3:19246709-19246728 None:intergenic 10.0%
!! AGAATAGATTAATAACAAAA+TGG - chr8.3:19247321-19247340 MS.gene37224:CDS 15.0%
!!! TACTTAAATAGTTTGTAAAT+TGG - chr8.3:19246650-19246669 MS.gene37224:intron 15.0%
!! ACCACAAACTATTTAATTAT+GGG + chr8.3:19246914-19246933 None:intergenic 20.0%
!! ATATAGTGTTATACCAAAAA+AGG + chr8.3:19246457-19246476 None:intergenic 20.0%
!! ATGTAGTTATGTGATATAAA+CGG - chr8.3:19247723-19247742 MS.gene37224:intron 20.0%
!! GGTAACTAATTAAAATTTGA+TGG - chr8.3:19246931-19246950 MS.gene37224:intron 20.0%
!! TACCACAAACTATTTAATTA+TGG + chr8.3:19246915-19246934 None:intergenic 20.0%
!! TTATGAGACATTAGAAAATA+TGG + chr8.3:19247351-19247370 None:intergenic 20.0%
!!! AAAGATCAAATAACTACTTT+TGG + chr8.3:19247623-19247642 None:intergenic 20.0%
!!! AAATTTGATGGACTTATTAA+AGG - chr8.3:19246943-19246962 MS.gene37224:intron 20.0%
!!! ATATCTCTTTTGTAATGTTA+AGG + chr8.3:19247945-19247964 None:intergenic 20.0%
!!! CTAAAATTTTGTGCATTTAA+TGG - chr8.3:19246790-19246809 MS.gene37224:intron 20.0%
!!! GATTTACCAAATTTAAAATG+TGG + chr8.3:19247432-19247451 None:intergenic 20.0%
!!! GATTTATCAAATTTGAAACA+TGG + chr8.3:19247565-19247584 None:intergenic 20.0%
!!! TAATTTAGTGAGATTGATTT+TGG + chr8.3:19246433-19246452 None:intergenic 20.0%
!!! TTAGAGAATATTTCTTCTTT+TGG + chr8.3:19246774-19246793 None:intergenic 20.0%
! AACACATTATGCATGAAAAA+AGG + chr8.3:19247890-19247909 None:intergenic 25.0%
! AGTTCAACATTGTTCAATAA+TGG - chr8.3:19247027-19247046 MS.gene37224:intron 25.0%
! GGGAAAATTAGTATTAATTG+TGG + chr8.3:19246688-19246707 None:intergenic 25.0%
! GTTCAACATTGTTCAATAAT+GGG - chr8.3:19247028-19247047 MS.gene37224:intron 25.0%
! TAATCTTGAAAGAAAGAGTT+TGG + chr8.3:19248225-19248244 None:intergenic 25.0%
! TCCCATAATTAAATAGTTTG+TGG - chr8.3:19246910-19246929 MS.gene37224:intron 25.0%
! TTACAAAAGAGATATGTCTT+TGG - chr8.3:19247950-19247969 MS.gene37224:intron 25.0%
! TTTCAGTTAGGATTCAAATT+CGG + chr8.3:19246541-19246560 None:intergenic 25.0%
!! ATAAGAGTCCATATTTTCTA+TGG - chr8.3:19247683-19247702 MS.gene37224:intron 25.0%
!!! AATGTCTGATTTTGATATGA+TGG + chr8.3:19247542-19247561 None:intergenic 25.0%
!!! AATTTGTTTCTGAATGGTTA+TGG - chr8.3:19246985-19247004 MS.gene37224:intron 25.0%
!!! ATTTGTTTCTGAATGGTTAT+GGG - chr8.3:19246986-19247005 MS.gene37224:intron 25.0%
!!! CTCACTAAATTAACCTTTTT+TGG - chr8.3:19246441-19246460 MS.gene37224:CDS 25.0%
!!! TATGGAAATTTGTTTCTGAA+TGG - chr8.3:19246979-19246998 MS.gene37224:intron 25.0%
AAAATTGACATGTGTGGAAT+AGG + chr8.3:19248317-19248336 None:intergenic 30.0%
ATCTGATCTTGATACAATCT+AGG - chr8.3:19247820-19247839 MS.gene37224:intron 30.0%
ATTGAGATATAACTTACCCA+TGG + chr8.3:19246361-19246380 None:intergenic 30.0%
CAAAGTTTGACCAACATTAT+TGG - chr8.3:19246597-19246616 MS.gene37224:intron 30.0%
CAATCATGGAAATGTCAATT+TGG + chr8.3:19247656-19247675 None:intergenic 30.0%
CATTGTGACCATAGAAAATA+TGG + chr8.3:19247694-19247713 None:intergenic 30.0%
CCAAAGAAACTATAATAGCT+TGG - chr8.3:19248257-19248276 MS.gene37224:CDS 30.0%
CCAAGCTATTATAGTTTCTT+TGG + chr8.3:19248260-19248279 None:intergenic 30.0%
GCATGAAAAAAGGTTGAATT+TGG + chr8.3:19247880-19247899 None:intergenic 30.0%
GGTCAAACATTATTTCAGTT+AGG + chr8.3:19246553-19246572 None:intergenic 30.0%
TAAACGGTTAAATCAAAGAG+AGG - chr8.3:19247739-19247758 MS.gene37224:intron 30.0%
TCAATGAAAATTGACATGTG+TGG + chr8.3:19248323-19248342 None:intergenic 30.0%
TTCAGTTAGGATTCAAATTC+GGG + chr8.3:19246540-19246559 None:intergenic 30.0%
TTGTACATCAATTGCTGAAA+TGG + chr8.3:19246409-19246428 None:intergenic 30.0%
! GTTGCATCATTTGTCTTTTA+AGG - chr8.3:19247907-19247926 MS.gene37224:intron 30.0%
!! TTTGATGGACTTATTAAAGG+AGG - chr8.3:19246946-19246965 MS.gene37224:intron 30.0%
AATTACCTGCGATACATAAC+AGG + chr8.3:19247267-19247286 None:intergenic 35.0%
AGGTGATATAGCAAACATGT+GGG - chr8.3:19248120-19248139 MS.gene37224:intron 35.0%
ATAACCAATACCCATTTCCT+TGG + chr8.3:19248072-19248091 None:intergenic 35.0%
ATGATAGTTCGTCAATCGTT+TGG + chr8.3:19246494-19246513 None:intergenic 35.0%
ATTACCTGCGATACATAACA+GGG + chr8.3:19247266-19247285 None:intergenic 35.0%
ATTAGGAGGGCAAATATTTG+TGG + chr8.3:19247136-19247155 None:intergenic 35.0%
CAAATATTTGCCCTCCTAAT+TGG - chr8.3:19247136-19247155 MS.gene37224:intron 35.0%
CTTGATTCAACATGATTGTG+TGG - chr8.3:19247486-19247505 MS.gene37224:CDS 35.0%
GATTCTCTTTCCAAGGAAAT+GGG - chr8.3:19248059-19248078 MS.gene37224:intron 35.0%
GCTATCGGATTAAAATCAGA+CGG - chr8.3:19247405-19247424 MS.gene37224:CDS 35.0%
TTAAAGGAAGTAGAACAGGA+TGG - chr8.3:19248143-19248162 MS.gene37224:intron 35.0%
TTTCTTAACATGTGCCCTAA+GGG + chr8.3:19246750-19246769 None:intergenic 35.0%
! ATAGCAAACATGTGGGTTAA+AGG - chr8.3:19248127-19248146 MS.gene37224:intron 35.0%
! GACGGTCCACATTTTAAATT+TGG - chr8.3:19247423-19247442 MS.gene37224:CDS 35.0%
! GATACAATCTAGGTTGACAA+CGG - chr8.3:19247830-19247849 MS.gene37224:intron 35.0%
! TTATTAAAGGAGGAGCATGT+GGG - chr8.3:19246956-19246975 MS.gene37224:intron 35.0%
! TTTTGTCACGTGATCTATCT+AGG - chr8.3:19247383-19247402 MS.gene37224:CDS 35.0%
!! AATCATTAACTTGTGCCTTG+AGG - chr8.3:19246872-19246891 MS.gene37224:intron 35.0%
!! AGGAAATGGGTATTGGTTAT+TGG - chr8.3:19248072-19248091 MS.gene37224:intron 35.0%
!! ATCATTAACTTGTGCCTTGA+GGG - chr8.3:19246873-19246892 MS.gene37224:intron 35.0%
!! TCAATAATGGGTATGCATGT+GGG - chr8.3:19247040-19247059 MS.gene37224:intron 35.0%
!! TTCAATAATGGGTATGCATG+TGG - chr8.3:19247039-19247058 MS.gene37224:intron 35.0%
AACCGCAGTTCCAATAATGT+TGG + chr8.3:19246610-19246629 None:intergenic 40.0%
ACGTGATCTATCTAGGCTAT+CGG - chr8.3:19247390-19247409 MS.gene37224:CDS 40.0%
ATACATAACAGGGACGATAC+CGG + chr8.3:19247256-19247275 None:intergenic 40.0%
ATGTCAAAATGTGAACGTGG+TGG + chr8.3:19247195-19247214 None:intergenic 40.0%
ATTAGCATGAGCCACAATTG+GGG - chr8.3:19248166-19248185 MS.gene37224:intron 40.0%
ATTGGTGTACGTGATGAATG+TGG - chr8.3:19248090-19248109 MS.gene37224:intron 40.0%
CATGAAAATTGCACAGTGGA+AGG - chr8.3:19247230-19247249 MS.gene37224:intron 40.0%
CCTTCATGAAAATTGCACAG+TGG - chr8.3:19247226-19247245 MS.gene37224:intron 40.0%
CGATTCTCTTTCCAAGGAAA+TGG - chr8.3:19248058-19248077 MS.gene37224:intron 40.0%
CTTTCCAAGGAAATGGGTAT+TGG - chr8.3:19248065-19248084 MS.gene37224:intron 40.0%
CTTTCTTAACATGTGCCCTA+AGG + chr8.3:19246751-19246770 None:intergenic 40.0%
GACATGTCAAAATGTGAACG+TGG + chr8.3:19247198-19247217 None:intergenic 40.0%
GACCAACATTATTGGAACTG+CGG - chr8.3:19246605-19246624 MS.gene37224:intron 40.0%
GACTGTAGTGTAAGGAACAT+TGG + chr8.3:19247109-19247128 None:intergenic 40.0%
GAGATATAACTTACCCATGG+TGG + chr8.3:19246358-19246377 None:intergenic 40.0%
GAGGTGATATAGCAAACATG+TGG - chr8.3:19248119-19248138 MS.gene37224:intron 40.0%
GATTAGCATGAGCCACAATT+GGG - chr8.3:19248165-19248184 MS.gene37224:intron 40.0%
GGATTTCGATTCTCTTTCCA+AGG - chr8.3:19248052-19248071 MS.gene37224:intron 40.0%
GGTAGTTCATGCTTCAACTT+TGG - chr8.3:19247760-19247779 MS.gene37224:intron 40.0%
TACCAAGCATTTGCAACACT+TGG - chr8.3:19248196-19248215 MS.gene37224:intron 40.0%
TGGGTTAAAGGAAGTAGAAC+AGG - chr8.3:19248139-19248158 MS.gene37224:intron 40.0%
TGTCAAAATGTGAACGTGGT+GGG + chr8.3:19247194-19247213 None:intergenic 40.0%
TGTCTCATCGCCATAAAATG+TGG + chr8.3:19246328-19246347 None:intergenic 40.0%
TTGACATTTCCATGATTGCG+TGG - chr8.3:19247658-19247677 MS.gene37224:intron 40.0%
TTGGTGTACGTGATGAATGT+GGG - chr8.3:19248091-19248110 MS.gene37224:intron 40.0%
! CCACTGTGCAATTTTCATGA+AGG + chr8.3:19247229-19247248 None:intergenic 40.0%
! CTTATTAAAGGAGGAGCATG+TGG - chr8.3:19246955-19246974 MS.gene37224:intron 40.0%
! CTTGCACATGCCACATTTTA+TGG - chr8.3:19246315-19246334 MS.gene37224:CDS 40.0%
! TGTGCAATTTTCATGAAGGC+AGG + chr8.3:19247225-19247244 None:intergenic 40.0%
!! AGTGTTAACTTGTGCCCTTA+GGG - chr8.3:19246733-19246752 MS.gene37224:intron 40.0%
!! TAGTGTTAACTTGTGCCCTT+AGG - chr8.3:19246732-19246751 MS.gene37224:intron 40.0%
AAAATTGCACAGTGGAAGGC+CGG - chr8.3:19247234-19247253 MS.gene37224:intron 45.0%
AATCCTTCACTCCTCATGCA+AGG + chr8.3:19248037-19248056 None:intergenic 45.0%
AATGTTAACCTGTGCCCTCA+AGG + chr8.3:19246890-19246909 None:intergenic 45.0%
ATGCCTGAGACCAATTAGGA+GGG + chr8.3:19247149-19247168 None:intergenic 45.0%
ATGTGCAAGAGTCCATGAAC+TGG + chr8.3:19246304-19246323 None:intergenic 45.0%
CAAGCATTTGCAACACTTGG+TGG - chr8.3:19248199-19248218 MS.gene37224:intron 45.0%
CTCTTATGTCCACGCAATCA+TGG + chr8.3:19247670-19247689 None:intergenic 45.0%
GAATGTTGCTCCTTCCAACT+GGG - chr8.3:19248279-19248298 MS.gene37224:CDS 45.0%
GCACATTTCAACCCAGTTCA+TGG - chr8.3:19246289-19246308 MS.gene37224:CDS 45.0%
TGTGCAAGAGTCCATGAACT+GGG + chr8.3:19246303-19246322 None:intergenic 45.0%
! CACCAAGTGTTGCAAATGCT+TGG + chr8.3:19248201-19248220 None:intergenic 45.0%
! GGATTAGCATGAGCCACAAT+TGG - chr8.3:19248164-19248183 MS.gene37224:intron 45.0%
! TGCAAAGCCAGCTTTAGTAG+CGG - chr8.3:19246266-19246285 MS.gene37224:CDS 45.0%
! TTCTTAGAGTGCCTTGCATG+AGG - chr8.3:19248023-19248042 MS.gene37224:intron 45.0%
!! AAAAAAAAATAAAATAATGT+TGG + chr8.3:19246574-19246593 None:intergenic 5.0%
AATGTGCCCGCTACTAAAGC+TGG + chr8.3:19246276-19246295 None:intergenic 50.0%
GATGCCTGAGACCAATTAGG+AGG + chr8.3:19247150-19247169 None:intergenic 50.0%
GGAATGTTGCTCCTTCCAAC+TGG - chr8.3:19248278-19248297 MS.gene37224:CDS 50.0%
GTGGCAGTGACTGTAGTGTA+AGG + chr8.3:19247117-19247136 None:intergenic 50.0%
GTGTACGTGATGAATGTGGG+AGG - chr8.3:19248094-19248113 MS.gene37224:intron 50.0%
GTTGCACCATCCACCGTAAT+CGG + chr8.3:19247172-19247191 None:intergenic 50.0%
TACGTGATGAATGTGGGAGG+TGG - chr8.3:19248097-19248116 MS.gene37224:intron 50.0%
TCGGATGCCTGAGACCAATT+AGG + chr8.3:19247153-19247172 None:intergenic 50.0%
TCGTCCCTGTTATGTATCGC+AGG - chr8.3:19247259-19247278 MS.gene37224:intron 50.0%
TGGTCTCAGGCATCCGATTA+CGG - chr8.3:19247156-19247175 MS.gene37224:intron 50.0%
! AAAGGAGGAGCATGTGGGTA+TGG - chr8.3:19246961-19246980 MS.gene37224:intron 50.0%
! GCAAAGCCAGCTTTAGTAGC+GGG - chr8.3:19246267-19246286 MS.gene37224:CDS 50.0%
! TTGCCCTCCTAATTGGTCTC+AGG - chr8.3:19247143-19247162 MS.gene37224:intron 50.0%
!! GGTAAGAAGCACCCCAATTG+TGG + chr8.3:19248180-19248199 None:intergenic 50.0%
AATCCAGCAGCCCAGTTGGA+AGG + chr8.3:19248292-19248311 None:intergenic 55.0%
AGGCATCCGATTACGGTGGA+TGG - chr8.3:19247163-19247182 MS.gene37224:intron 55.0%
GAGACAGCTTCTGCCACCAT+GGG - chr8.3:19246342-19246361 MS.gene37224:CDS 55.0%
GGTTAATCCAGCAGCCCAGT+TGG + chr8.3:19248296-19248315 None:intergenic 55.0%
GTGATGAATGTGGGAGGTGG+AGG - chr8.3:19248100-19248119 MS.gene37224:intron 55.0%
GTGCCTTGCATGAGGAGTGA+AGG - chr8.3:19248031-19248050 MS.gene37224:intron 55.0%
TCTCAGGCATCCGATTACGG+TGG - chr8.3:19247159-19247178 MS.gene37224:intron 55.0%
TGAGACAGCTTCTGCCACCA+TGG - chr8.3:19246341-19246360 MS.gene37224:CDS 55.0%
! AACTTGTGCCTTGAGGGCAC+AGG - chr8.3:19246879-19246898 MS.gene37224:intron 55.0%
GCTCCTTCCAACTGGGCTGC+TGG - chr8.3:19248286-19248305 MS.gene37224:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 19246252 19248342 19246252 ID=MS.gene37224
chr8.3 mRNA 19246252 19248342 19246252 ID=MS.gene37224.t1;Parent=MS.gene37224
chr8.3 exon 19248231 19248342 19248231 ID=MS.gene37224.t1.exon1;Parent=MS.gene37224.t1
chr8.3 CDS 19248231 19248342 19248231 ID=cds.MS.gene37224.t1;Parent=MS.gene37224.t1
chr8.3 exon 19247314 19247629 19247314 ID=MS.gene37224.t1.exon2;Parent=MS.gene37224.t1
chr8.3 CDS 19247314 19247629 19247314 ID=cds.MS.gene37224.t1;Parent=MS.gene37224.t1
chr8.3 exon 19246252 19246564 19246252 ID=MS.gene37224.t1.exon3;Parent=MS.gene37224.t1
chr8.3 CDS 19246252 19246564 19246252 ID=cds.MS.gene37224.t1;Parent=MS.gene37224.t1
Gene Sequence

>MS.gene37224

ATGCTCACAATCATTGCAAAGCCAGCTTTAGTAGCGGGCACATTTCAACCCAGTTCATGGACTCTTGCACATGCCACATTTTATGGCGATGAGACAGCTTCTGCCACCATGGGTAAGTTATATCTCAATCTCTTAATTATTATCATTAGCTAAACCATTTCAGCAATTGATGTACAAGCCAAAATCAATCTCACTAAATTAACCTTTTTTGGTATAACACTATATTAACATTTTACTGACCAAACGATTGACGAACTATCATTTAAAACAAATTATTCAGAAGAACCCGAATTTGAATCCTAACTGAAATAATGTTTGACCAACATTATTTTATTTTTTTTTTGACAAAGTTTGACCAACATTATTGGAACTGCGGTTAAGACAAACATTTAACATCTTACTTAAATAGTTTGTAAATTGGTCATTAGCATTACCACAATTAATACTAATTTTCCCTTCTAGATATAAATAATTAAAGGGTAGTGTTAACTTGTGCCCTTAGGGCACATGTTAAGAAAGCCAAAAGAAGAAATATTCTCTAAAATTTTGTGCATTTAATGGATTAAAAAGTTAAAAATTAAATTCAATGTACACATTCCAATATATTATTTCTATATTTGAATCATTAACTTGTGCCTTGAGGGCACAGGTTAACATTTCCCATAATTAAATAGTTTGTGGTAACTAATTAAAATTTGATGGACTTATTAAAGGAGGAGCATGTGGGTATGGAAATTTGTTTCTGAATGGTTATGGGACAGACACAGCAGCATTGAGTTCAACATTGTTCAATAATGGGTATGCATGTGGGACTTGTTATCAAATAAAATGTGTTCAATCAAGTGCATGTAAGACCAATGTTCCTTACACTACAGTCACTGCCACAAATATTTGCCCTCCTAATTGGTCTCAGGCATCCGATTACGGTGGATGGTGCAACCCACCACGTTCACATTTTGACATGTCTAAGCCTGCCTTCATGAAAATTGCACAGTGGAAGGCCGGTATCGTCCCTGTTATGTATCGCAGGTAATTAACAAATCAAGCTTGCTCCACATATATATAATATAGAATAGATTAATAACAAAATGGTCGTCCATATTTTCTAATGTCTCATAAACATGCATATGATTTTGTCACGTGATCTATCTAGGCTATCGGATTAAAATCAGACGGTCCACATTTTAAATTTGGTAAATCAAATTTAAAGTACAAAGTGAAAATGTGATCTAATCTTGATTCAACATGATTGTGTGGATACATATATTTACAGTCACATGATTTTGACCATCATATCAAAATCAGACATTCCATGTTTCAAATTTGATAAATCGAGACATTCCATGTTTCAAATTTGATAAATCGAGTCCAAAAGTAGTTATTTGATCTTTATCTCGTAATCCAAATTGACATTTCCATGATTGCGTGGACATAAGAGTCCATATTTTCTATGGTCACAATGACACAATTGATGTAGTTATGTGATATAAACGGTTAAATCAAAGAGAGGTAGTTCATGCTTCAACTTTGGTAAATTGCGTCTAAAATGCAAAGTTGAAATATCAATAATCTGATCTTGATACAATCTAGGTTGACAACGGCACGATTGTGTGATCACAAAGAATCCAAATTCAACCTTTTTTCATGCATAATGTGTTGCATCATTTGTCTTTTAAGGAGATTATTCTTACCTTAACATTACAAAAGAGATATGTCTTTGGCTTTAGTGATATCGTACTTACAATGTGATTTGTTGATAAAAAAATTGAATTTCTTAGAGTGCCTTGCATGAGGAGTGAAGGATTTCGATTCTCTTTCCAAGGAAATGGGTATTGGTTATTGGTGTACGTGATGAATGTGGGAGGTGGAGGTGATATAGCAAACATGTGGGTTAAAGGAAGTAGAACAGGATGGATTAGCATGAGCCACAATTGGGGTGCTTCTTACCAAGCATTTGCAACACTTGGTGGCCAAACTCTTTCTTTCAAGATTACTTCTTACACAACCAAAGAAACTATAATAGCTTGGAATGTTGCTCCTTCCAACTGGGCTGCTGGATTAACCTATTCCACACATGTCAATTTTCATTGA
Protein sequence

>MS.gene37224.t1

MLTIIAKPALVAGTFQPSSWTLAHATFYGDETASATMGGACGYGNLFLNGYGTDTAALSSTLFNNGYACGTCYQIKCVQSSACKTNVPYTTVTATNICPPNWSQASDYGGWCNPPRSHFDMSKPAFMKIAQWKAGIVPVMYRRVPCMRSEGFRFSFQGNGYWLLVYVMNVGGGGDIANMWVKGSRTGWISMSHNWGASYQAFATLGGQTLSFKITSYTTKETIIAWNVAPSNWAAGLTYSTHVNFH