AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033403

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033403.t1 MTR_4g102450 95.455 264 10 1 1 264 1 262 0.0 518
MS.gene033403.t1 MTR_5g011210 76.894 264 60 1 1 264 1 263 3.56e-154 430
MS.gene033403.t1 MTR_5g079950 53.333 255 116 3 11 264 6 258 3.94e-95 280
MS.gene033403.t1 MTR_2g097030 50.195 257 125 3 9 264 5 259 1.42e-92 274
MS.gene033403.t1 MTR_7g061720 51.172 256 121 4 11 264 4 257 1.53e-89 266
MS.gene033403.t1 MTR_4g063975 50.189 265 123 5 1 264 1 257 4.89e-87 259
MS.gene033403.t1 MTR_7g111010 50.391 256 119 4 11 263 4 254 4.33e-86 257
MS.gene033403.t1 MTR_1g085460 53.219 233 105 3 34 263 26 257 7.00e-86 256
MS.gene033403.t1 MTR_5g041700 51.600 250 110 5 11 258 3 243 1.67e-85 255
MS.gene033403.t1 MTR_7g110990 55.022 229 99 3 38 263 12 239 7.36e-85 253
MS.gene033403.t1 MTR_3g094040 50.198 253 118 5 9 258 2 249 2.53e-84 253
MS.gene033403.t1 MTR_4g081950 55.605 223 93 4 38 258 61 279 2.79e-84 253
MS.gene033403.t1 MTR_3g094060 50.988 253 111 5 9 258 7 249 2.98e-84 252
MS.gene033403.t1 MTR_3g435430 54.709 223 95 4 38 258 25 243 3.74e-83 249
MS.gene033403.t1 MTR_7g011060 51.230 244 107 4 16 258 4 236 6.64e-82 246
MS.gene033403.t1 MTR_5g075320 53.394 221 99 3 38 257 29 246 5.21e-81 244
MS.gene033403.t1 MTR_8g058990 52.609 230 103 4 36 263 28 253 5.49e-81 244
MS.gene033403.t1 MTR_7g013140 49.606 254 118 4 11 263 7 251 3.18e-80 242
MS.gene033403.t1 MTR_8g464350 47.490 259 125 5 7 263 5 254 5.01e-80 241
MS.gene033403.t1 MTR_1g026020 50.215 233 113 2 33 263 24 255 7.10e-80 241
MS.gene033403.t1 MTR_4g124340 47.899 238 119 4 22 257 45 279 1.26e-79 242
MS.gene033403.t1 MTR_3g466760 47.104 259 133 3 1 258 1 256 2.02e-79 240
MS.gene033403.t1 MTR_7g013120 49.412 255 119 5 11 263 7 253 2.26e-79 240
MS.gene033403.t1 MTR_3g466790 49.600 250 120 4 9 257 5 249 1.73e-78 238
MS.gene033403.t1 MTR_7g013150 50.877 228 108 3 37 263 29 253 1.32e-76 233
MS.gene033403.t1 MTR_0040s0140 44.628 242 130 3 18 258 14 252 2.69e-69 214
MS.gene033403.t1 MTR_6g012520 50.000 250 112 4 9 258 2 238 4.92e-69 213
MS.gene033403.t1 MTR_2g090255 40.496 242 142 2 18 258 8 248 7.21e-66 206
MS.gene033403.t1 MTR_5g076950 46.188 223 119 1 36 258 39 260 6.27e-65 204
MS.gene033403.t1 MTR_3g064510 45.148 237 109 7 36 258 51 280 1.74e-60 193
MS.gene033403.t1 MTR_0008s0120 33.772 228 93 4 36 263 30 199 1.86e-32 118
MS.gene033403.t1 MTR_2g090270 30.712 267 154 10 10 263 10 258 2.91e-32 119
MS.gene033403.t1 MTR_0122s0020 31.047 277 158 11 6 264 4 265 9.70e-32 118
MS.gene033403.t1 MTR_2g090265 31.557 244 138 10 33 263 29 256 8.62e-30 112
MS.gene033403.t1 MTR_4g028640 34.568 243 124 10 38 264 42 265 1.91e-27 107
MS.gene033403.t1 MTR_6g012500 45.361 97 52 1 162 258 181 276 5.68e-22 92.8
MS.gene033403.t1 MTR_0118s0070 29.237 236 139 8 43 263 53 275 1.25e-21 91.7
MS.gene033403.t1 MTR_5g013440 25.292 257 170 8 16 264 10 252 3.27e-21 90.1
MS.gene033403.t1 MTR_0809s0010 29.268 246 142 9 33 263 42 270 6.17e-21 89.7
MS.gene033403.t1 MTR_0122s0020 30.698 215 118 9 6 203 4 204 8.97e-21 87.8
MS.gene033403.t1 MTR_4g099370 28.302 265 152 10 16 264 9 251 6.02e-20 86.7
MS.gene033403.t1 MTR_4g075790 28.194 227 132 7 51 263 34 243 1.97e-16 77.0
MS.gene033403.t1 MTR_4g098930 26.966 267 156 10 10 261 8 250 2.32e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033403.t1 AT1G12560 64.394 264 92 2 1 264 1 262 7.06e-124 353
MS.gene033403.t1 AT1G62980 64.777 247 87 0 18 264 11 257 3.30e-120 344
MS.gene033403.t1 AT3G55500 54.618 249 110 3 11 257 6 253 1.34e-94 279
MS.gene033403.t1 AT2G39700 53.226 248 113 3 11 257 5 250 2.31e-93 276
MS.gene033403.t1 AT5G02260 50.201 249 122 2 11 258 5 252 1.65e-89 266
MS.gene033403.t1 AT2G37640 52.033 246 116 2 14 258 12 256 1.95e-89 266
MS.gene033403.t1 AT2G28950 49.799 249 122 3 11 257 3 250 2.42e-88 263
MS.gene033403.t1 AT4G01630 54.701 234 92 4 38 263 28 255 6.52e-88 262
MS.gene033403.t1 AT1G69530 51.200 250 115 5 11 258 1 245 2.04e-85 255
MS.gene033403.t1 AT1G69530 51.200 250 115 5 11 258 1 245 2.04e-85 255
MS.gene033403.t1 AT1G69530 50.794 252 117 5 11 260 1 247 3.11e-85 256
MS.gene033403.t1 AT5G56320 52.016 248 111 5 12 258 7 247 5.63e-85 254
MS.gene033403.t1 AT5G56320 52.016 248 111 5 12 258 7 247 5.63e-85 254
MS.gene033403.t1 AT2G03090 55.605 223 97 2 36 258 28 248 7.16e-85 254
MS.gene033403.t1 AT5G56320 52.016 248 111 5 12 258 7 247 3.50e-84 254
MS.gene033403.t1 AT2G40610 51.037 241 113 4 19 257 11 248 5.59e-82 246
MS.gene033403.t1 AT1G20190 50.000 238 116 2 28 263 16 252 1.03e-79 241
MS.gene033403.t1 AT2G37640 54.634 205 91 2 55 258 28 231 7.37e-79 238
MS.gene033403.t1 AT5G05290 51.339 224 102 5 38 258 32 251 2.51e-78 237
MS.gene033403.t1 AT5G39300 45.455 275 129 7 4 263 8 276 3.31e-78 238
MS.gene033403.t1 AT5G39280 48.207 251 118 6 19 263 31 275 2.06e-77 236
MS.gene033403.t1 AT1G26770 49.213 254 115 5 7 258 3 244 8.39e-77 233
MS.gene033403.t1 AT5G39270 50.000 230 107 5 36 263 56 279 1.14e-76 234
MS.gene033403.t1 AT1G26770 49.213 254 115 5 7 258 13 254 1.22e-76 233
MS.gene033403.t1 AT1G69530 50.000 236 108 6 11 244 1 228 4.91e-76 231
MS.gene033403.t1 AT5G39290 50.218 229 106 5 37 263 57 279 1.55e-75 231
MS.gene033403.t1 AT3G29030 45.703 256 121 3 12 258 3 249 7.01e-75 229
MS.gene033403.t1 AT1G69530 52.036 221 98 5 11 229 1 215 1.05e-74 227
MS.gene033403.t1 AT5G39310 45.607 239 117 7 30 263 82 312 2.40e-68 214
MS.gene033403.t1 AT3G03220 44.889 225 117 4 37 258 39 259 1.38e-63 200
MS.gene033403.t1 AT3G15370 44.578 249 126 6 17 260 6 247 1.41e-63 200
MS.gene033403.t1 AT4G38210 42.060 233 130 4 35 264 25 255 2.93e-61 194
MS.gene033403.t1 AT1G20190 48.187 193 97 2 73 263 2 193 1.50e-60 190
MS.gene033403.t1 AT3G15370 39.785 279 126 7 17 260 6 277 3.42e-57 184
MS.gene033403.t1 AT5G39260 42.241 232 118 6 36 263 43 262 6.56e-55 178
MS.gene033403.t1 AT4G28250 32.593 270 150 10 10 263 7 260 1.41e-35 128
MS.gene033403.t1 AT4G28250 31.955 266 151 9 10 263 7 254 3.42e-34 124
MS.gene033403.t1 AT1G65680 32.800 250 141 8 28 263 37 273 3.66e-33 122
MS.gene033403.t1 AT2G20750 31.818 242 139 8 36 263 38 267 2.52e-32 120
MS.gene033403.t1 AT2G45110 30.576 278 159 11 1 263 1 259 9.34e-28 107
MS.gene033403.t1 AT1G65681 31.556 225 129 7 52 264 12 223 1.99e-25 100
MS.gene033403.t1 AT4G28250 30.392 204 115 7 10 203 7 193 5.72e-21 88.6
MS.gene033403.t1 AT4G28250 30.846 201 112 7 10 200 7 190 7.02e-21 88.2
MS.gene033403.t1 AT2G20750 30.319 188 107 7 36 211 38 213 7.85e-20 85.5
MS.gene033403.t1 AT3G60570 26.217 267 166 8 10 263 9 257 2.55e-19 85.1
MS.gene033403.t1 AT4G17030 26.087 253 168 7 17 261 6 247 2.78e-19 84.7
MS.gene033403.t1 AT3G60570 26.244 221 135 7 56 263 4 209 2.20e-14 70.9
MS.gene033403.t1 AT3G45970 25.806 217 141 6 57 263 15 221 1.69e-12 65.9

Find 78 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCAACATTGTTCAATAA+TGG 0.212841 8.4:-22557469 MS.gene033403:CDS
CCAAGCTATTATAGTTTCTT+TGG 0.229810 8.4:+22556239 None:intergenic
TTATCAATATTGTAGCTTCT+TGG 0.253481 8.4:-22558089 MS.gene033403:CDS
AGGAAATGGGTATTGGTTAT+TGG 0.261742 8.4:-22556424 MS.gene033403:CDS
CAAATATTTGCCCTCCTAAT+TGG 0.264760 8.4:-22557360 MS.gene033403:CDS
CGATTCTCTTTCCAAGGAAA+TGG 0.277433 8.4:-22556438 MS.gene033403:CDS
GGAATGTTGCTCCTTCCAAC+TGG 0.286104 8.4:-22556218 MS.gene033403:CDS
GCTCCTTCCAACTGGGCTGC+TGG 0.323833 8.4:-22556210 MS.gene033403:CDS
GTTCAACATTGTTCAATAAT+GGG 0.335116 8.4:-22557468 MS.gene033403:CDS
CAAGCATTTGCAACACTTAG+TGG 0.353072 8.4:-22556297 MS.gene033403:CDS
AATTTGTTTCTGAATGGTTA+TGG 0.357643 8.4:-22557511 MS.gene033403:CDS
AATTACCTGCGATACATAAC+AGG 0.358374 8.4:+22557232 None:intergenic
TAATCTTGAAAGAAAGAGTT+TGG 0.362500 8.4:+22556274 None:intergenic
GCACATTTCAACCCAGTTCA+TGG 0.362883 8.4:-22558009 MS.gene033403:CDS
CTTTCCAAGGAAATGGGTAT+TGG 0.363273 8.4:-22556431 MS.gene033403:CDS
ATTTGTTTCTGAATGGTTAT+GGG 0.373242 8.4:-22557510 MS.gene033403:CDS
AAAATTGACATGTGTGGAAT+AGG 0.412567 8.4:+22556182 None:intergenic
GGATTAGCATGAGCCACAAT+TGG 0.416127 8.4:-22556332 MS.gene033403:CDS
TCGGATGCCTGAGACCAATT+AGG 0.422103 8.4:+22557346 None:intergenic
TGGTCTCAGGCATCCGATTA+CGG 0.441938 8.4:-22557340 MS.gene033403:CDS
ATAACCAATACCCATTTCCT+TGG 0.444503 8.4:+22556427 None:intergenic
GATTAGCATGAGCCACAATT+GGG 0.448115 8.4:-22556331 MS.gene033403:CDS
TGGGTGAAAGGAAGCAGAAC+AGG 0.453444 8.4:-22556357 MS.gene033403:CDS
GATTCTCTTTCCAAGGAAAT+GGG 0.454324 8.4:-22556437 MS.gene033403:CDS
TTGCCCTCCTAATTGGTCTC+AGG 0.458583 8.4:-22557353 MS.gene033403:CDS
GTTGCACCATCCACCGTAAT+CGG 0.465140 8.4:+22557327 None:intergenic
TATGGAAATTTGTTTCTGAA+TGG 0.491875 8.4:-22557517 MS.gene033403:CDS
AAAGGAGGAGCATGTGGGTA+TGG 0.494882 8.4:-22557535 MS.gene033403:intron
ATTAGGAGGGCAAATATTTG+TGG 0.499330 8.4:+22557363 None:intergenic
GCAAAGCCAGCTTTAGTAGC+GGG 0.501290 8.4:-22558031 MS.gene033403:CDS
GAATGTTGCTCCTTCCAACT+GGG 0.505072 8.4:-22556217 MS.gene033403:CDS
ATGTGCAAGAGTCCATGAAC+TGG 0.507085 8.4:+22557997 None:intergenic
AATGTGCCCGCTACTAAAGC+TGG 0.511601 8.4:+22558025 None:intergenic
GTGATGAATGTGGGAGGTGG+AGG 0.515112 8.4:-22556396 MS.gene033403:CDS
TACGTGATGAATGTGGGAGG+TGG 0.521955 8.4:-22556399 MS.gene033403:CDS
TGCAAAGCCAGCTTTAGTAG+CGG 0.523369 8.4:-22558032 MS.gene033403:CDS
CTTATTAAAGGAGGAGCATG+TGG 0.525297 8.4:-22557541 MS.gene033403:intron
CACTAAGTGTTGCAAATGCT+TGG 0.527699 8.4:+22556298 None:intergenic
GGATTTCGATTCTCTTTCCA+AGG 0.530783 8.4:-22556444 MS.gene033403:CDS
CCAAAGAAACTATAATAGCT+TGG 0.533024 8.4:-22556239 MS.gene033403:CDS
ATAGCAAACATGTGGGTGAA+AGG 0.540538 8.4:-22556369 MS.gene033403:CDS
AAAATTGCACAGTGGAAGGC+CGG 0.541507 8.4:-22557262 MS.gene033403:CDS
GGTTAATCCAGCAGCCCAGT+TGG 0.542121 8.4:+22556203 None:intergenic
ATACATAACAGGGACGATAC+CGG 0.542921 8.4:+22557243 None:intergenic
CATGAAAATTGCACAGTGGA+AGG 0.550705 8.4:-22557266 MS.gene033403:CDS
GGTAAGAAGCACCCCAATTG+TGG 0.559335 8.4:+22556319 None:intergenic
ATTGGTGTACGTGATGAATG+TGG 0.559419 8.4:-22556406 MS.gene033403:CDS
TTCAATAATGGGTATGCATG+TGG 0.563755 8.4:-22557457 MS.gene033403:CDS
AATCCAGCAGCCCAGTTGGA+AGG 0.563994 8.4:+22556207 None:intergenic
AGGCATCCGATTACGGTGGA+TGG 0.564250 8.4:-22557333 MS.gene033403:CDS
TTCTTAGAGTACCTTGCATG+AGG 0.569544 8.4:-22556473 MS.gene033403:intron
TCGTCCCTGTTATGTATCGC+AGG 0.570292 8.4:-22557237 MS.gene033403:intron
TTGGTGTACGTGATGAATGT+GGG 0.577309 8.4:-22556405 MS.gene033403:CDS
GATGCCTGAGACCAATTAGG+AGG 0.590186 8.4:+22557349 None:intergenic
GAGACAGCTTCTGCCACCAT+GGG 0.594645 8.4:-22557956 MS.gene033403:intron
GTACCTTGCATGAGGAGTGA+AGG 0.601544 8.4:-22556465 MS.gene033403:CDS
GACTGTAGTGTAAGGAACAT+TGG 0.607957 8.4:+22557390 None:intergenic
TGAGACAGCTTCTGCCACCA+TGG 0.614492 8.4:-22557957 MS.gene033403:CDS
TGAAAGGAAGCAGAACAGGA+TGG 0.625521 8.4:-22556353 MS.gene033403:CDS
TCAATAATGGGTATGCATGT+GGG 0.626106 8.4:-22557456 MS.gene033403:CDS
TGTCTCATCGCCATAAAATG+TGG 0.626462 8.4:+22557973 None:intergenic
GTGGCAGTGACTGTAGTGTA+AGG 0.632028 8.4:+22557382 None:intergenic
AATCCTTCACTCCTCATGCA+AGG 0.639387 8.4:+22556462 None:intergenic
ATGCCTGAGACCAATTAGGA+GGG 0.649082 8.4:+22557350 None:intergenic
GAGGTGATATAGCAAACATG+TGG 0.661138 8.4:-22556377 MS.gene033403:CDS
TTATTAAAGGAGGAGCATGT+GGG 0.672893 8.4:-22557540 MS.gene033403:intron
TGTGCAAGAGTCCATGAACT+GGG 0.678559 8.4:+22557998 None:intergenic
ATTAGCATGAGCCACAATTG+GGG 0.689678 8.4:-22556330 MS.gene033403:CDS
TCAATGAAAATTGACATGTG+TGG 0.690286 8.4:+22556176 None:intergenic
GTGTACGTGATGAATGTGGG+AGG 0.693958 8.4:-22556402 MS.gene033403:CDS
TCTCAGGCATCCGATTACGG+TGG 0.700337 8.4:-22557337 MS.gene033403:CDS
GAGATATAACTTACCCATGG+TGG 0.711843 8.4:+22557943 None:intergenic
TGTCAAAATGTGAACGTGGT+GGG 0.713445 8.4:+22557305 None:intergenic
ATGTCAAAATGTGAACGTGG+TGG 0.718706 8.4:+22557304 None:intergenic
ATTACCTGCGATACATAACA+GGG 0.735393 8.4:+22557233 None:intergenic
AGGTGATATAGCAAACATGT+GGG 0.736453 8.4:-22556376 MS.gene033403:CDS
CCTTCATGAAAATTGCACAG+TGG 0.762866 8.4:-22557270 MS.gene033403:CDS
GACATGTCAAAATGTGAACG+TGG 0.763719 8.4:+22557301 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAATTAAATAGTTTG+TGG - chr8.4:22556690-22556709 MS.gene033403:intron 10.0%
!! TTAATTATTTATATCTAGAA+GGG + chr8.4:22556682-22556701 None:intergenic 10.0%
!! TTTAATTATTTATATCTAGA+AGG + chr8.4:22556683-22556702 None:intergenic 10.0%
!! AGAATAGATTAATAACAAAA+TGG - chr8.4:22557101-22557120 MS.gene033403:intron 15.0%
!! TATTCTATATTATGTATATG+TGG + chr8.4:22557087-22557106 None:intergenic 15.0%
!!! TACTTAAATAGTTTGTAAAT+TGG - chr8.4:22556624-22556643 MS.gene033403:intron 15.0%
!! AACATATTATGCAAGAAAAA+AGG + chr8.4:22557670-22557689 None:intergenic 20.0%
!! ATATAGTGTTATACCAAAAA+AGG + chr8.4:22556435-22556454 None:intergenic 20.0%
!! GGTAACTAATTAAAATTTGA+TGG - chr8.4:22556711-22556730 MS.gene033403:intron 20.0%
!! TTATGAGACATTAGAAAATA+TGG + chr8.4:22557131-22557150 None:intergenic 20.0%
!! TTGACAAAGTTTAACATTAT+TGG - chr8.4:22556571-22556590 MS.gene033403:intron 20.0%
!!! AAAGATCAAATAACTACTTT+TGG + chr8.4:22557403-22557422 None:intergenic 20.0%
!!! AAATTTGATGGACTTATTAA+AGG - chr8.4:22556723-22556742 MS.gene033403:intron 20.0%
!!! ATATCTCTTTTGTAATGTTA+AGG + chr8.4:22557725-22557744 None:intergenic 20.0%
!!! GATTTACCAAATTTAAAATG+TGG + chr8.4:22557212-22557231 None:intergenic 20.0%
!!! GATTTATCAAATTTGAAACA+TGG + chr8.4:22557345-22557364 None:intergenic 20.0%
!!! TAATTTAGTGAGATTGATTT+TGG + chr8.4:22556411-22556430 None:intergenic 20.0%
! AGTTCAACATTGTTCAATAA+TGG - chr8.4:22556807-22556826 MS.gene033403:intron 25.0%
! ATGCAGTTATGTGATATAAA+CGG - chr8.4:22557503-22557522 MS.gene033403:CDS 25.0%
! GGGAAAATTAGTATTAATTG+TGG + chr8.4:22556662-22556681 None:intergenic 25.0%
! GTTCAACATTGTTCAATAAT+GGG - chr8.4:22556808-22556827 MS.gene033403:intron 25.0%
! TAATCTTGAAAGAAAGAGTT+TGG + chr8.4:22558005-22558024 None:intergenic 25.0%
! TTACAAAAGAGATATGTCTT+TGG - chr8.4:22557730-22557749 MS.gene033403:intron 25.0%
! TTATCAATATTGTAGCTTCT+TGG - chr8.4:22556187-22556206 MS.gene033403:CDS 25.0%
!! ATAAGAGTCCATATTTTCTA+TGG - chr8.4:22557463-22557482 MS.gene033403:CDS 25.0%
!!! AATGTCTGATTTTGATATGA+TGG + chr8.4:22557322-22557341 None:intergenic 25.0%
!!! AATTTGTTTCTGAATGGTTA+TGG - chr8.4:22556765-22556784 MS.gene033403:intron 25.0%
!!! ATTTGTTTCTGAATGGTTAT+GGG - chr8.4:22556766-22556785 MS.gene033403:intron 25.0%
!!! CTCACTAAATTAACCTTTTT+TGG - chr8.4:22556419-22556438 MS.gene033403:CDS 25.0%
!!! TATGGAAATTTGTTTCTGAA+TGG - chr8.4:22556759-22556778 MS.gene033403:intron 25.0%
AAAATTGACATGTGTGGAAT+AGG + chr8.4:22558097-22558116 None:intergenic 30.0%
ATCCAATCTTGATACAATCT+AGG - chr8.4:22557600-22557619 MS.gene033403:intron 30.0%
ATTGAGATATAACTTACCCA+TGG + chr8.4:22556339-22556358 None:intergenic 30.0%
CATTGTGACCATAGAAAATA+TGG + chr8.4:22557474-22557493 None:intergenic 30.0%
CCAAAGAAACTATAATAGCT+TGG - chr8.4:22558037-22558056 MS.gene033403:CDS 30.0%
CCAAGCTATTATAGTTTCTT+TGG + chr8.4:22558040-22558059 None:intergenic 30.0%
GCAAGAAAAAAGGTTGAATT+TGG + chr8.4:22557660-22557679 None:intergenic 30.0%
GCTATTGGATTAAAATCAGA+CGG - chr8.4:22557185-22557204 MS.gene033403:intron 30.0%
GTTTAACATTATTGGAACTG+CGG - chr8.4:22556579-22556598 MS.gene033403:intron 30.0%
TAAACGGTTAAATCAAAGAG+AGG - chr8.4:22557519-22557538 MS.gene033403:CDS 30.0%
TACCTAGATTGTATCAAGAT+TGG + chr8.4:22557605-22557624 None:intergenic 30.0%
TCAATGAAAATTGACATGTG+TGG + chr8.4:22558103-22558122 None:intergenic 30.0%
TGTTGGTCAAACATTATTTC+AGG + chr8.4:22556535-22556554 None:intergenic 30.0%
TTGTACATCAATTGCTGAAA+TGG + chr8.4:22556387-22556406 None:intergenic 30.0%
! GTTGCATCATTTGTCTTTTA+TGG - chr8.4:22557687-22557706 MS.gene033403:intron 30.0%
! TTTCAGGTAGGATTCAAATT+CGG + chr8.4:22556519-22556538 None:intergenic 30.0%
!! TTTGATGGACTTATTAAAGG+AGG - chr8.4:22556726-22556745 MS.gene033403:intron 30.0%
AATTACCTGCGATACATAAC+AGG + chr8.4:22557047-22557066 None:intergenic 35.0%
AGGTGATATAGCAAACATGT+GGG - chr8.4:22557900-22557919 MS.gene033403:intron 35.0%
ATAACCAATACCCATTTCCT+TGG + chr8.4:22557852-22557871 None:intergenic 35.0%
ATGATAGTTCGTCAATCGTT+TGG + chr8.4:22556472-22556491 None:intergenic 35.0%
ATTACCTGCGATACATAACA+GGG + chr8.4:22557046-22557065 None:intergenic 35.0%
ATTAGGAGGGCAAATATTTG+TGG + chr8.4:22556916-22556935 None:intergenic 35.0%
CAAATATTTGCCCTCCTAAT+TGG - chr8.4:22556916-22556935 MS.gene033403:intron 35.0%
CAATCATGCAGATGTCAATT+TGG + chr8.4:22557436-22557455 None:intergenic 35.0%
CTTGATTCAACATGATTGTG+TGG - chr8.4:22557266-22557285 MS.gene033403:CDS 35.0%
GATTCTCTTTCCAAGGAAAT+GGG - chr8.4:22557839-22557858 MS.gene033403:intron 35.0%
TTCAGGTAGGATTCAAATTC+GGG + chr8.4:22556518-22556537 None:intergenic 35.0%
! GACGGTCCACATTTTAAATT+TGG - chr8.4:22557203-22557222 MS.gene033403:intron 35.0%
! GGTCAAACATTATTTCAGGT+AGG + chr8.4:22556531-22556550 None:intergenic 35.0%
! TTATTAAAGGAGGAGCATGT+GGG - chr8.4:22556736-22556755 MS.gene033403:intron 35.0%
! TTTTGTCACGTGATCTATCT+AGG - chr8.4:22557163-22557182 MS.gene033403:intron 35.0%
!! AGGAAATGGGTATTGGTTAT+TGG - chr8.4:22557852-22557871 MS.gene033403:intron 35.0%
!! TCAATAATGGGTATGCATGT+GGG - chr8.4:22556820-22556839 MS.gene033403:intron 35.0%
!! TTCAATAATGGGTATGCATG+TGG - chr8.4:22556819-22556838 MS.gene033403:intron 35.0%
ACGTGATCTATCTAGGCTAT+TGG - chr8.4:22557170-22557189 MS.gene033403:intron 40.0%
ATACATAACAGGGACGATAC+CGG + chr8.4:22557036-22557055 None:intergenic 40.0%
ATAGCAAACATGTGGGTGAA+AGG - chr8.4:22557907-22557926 MS.gene033403:intron 40.0%
ATGTCAAAATGTGAACGTGG+TGG + chr8.4:22556975-22556994 None:intergenic 40.0%
ATTAGCATGAGCCACAATTG+GGG - chr8.4:22557946-22557965 MS.gene033403:intron 40.0%
ATTGGTGTACGTGATGAATG+TGG - chr8.4:22557870-22557889 MS.gene033403:intron 40.0%
CAAGCATTTGCAACACTTAG+TGG - chr8.4:22557979-22557998 MS.gene033403:CDS 40.0%
CATGAAAATTGCACAGTGGA+AGG - chr8.4:22557010-22557029 MS.gene033403:intron 40.0%
CCTTCATGAAAATTGCACAG+TGG - chr8.4:22557006-22557025 MS.gene033403:intron 40.0%
CGATTCTCTTTCCAAGGAAA+TGG - chr8.4:22557838-22557857 MS.gene033403:intron 40.0%
CTTTCCAAGGAAATGGGTAT+TGG - chr8.4:22557845-22557864 MS.gene033403:intron 40.0%
GACATGTCAAAATGTGAACG+TGG + chr8.4:22556978-22556997 None:intergenic 40.0%
GACTGTAGTGTAAGGAACAT+TGG + chr8.4:22556889-22556908 None:intergenic 40.0%
GAGATATAACTTACCCATGG+TGG + chr8.4:22556336-22556355 None:intergenic 40.0%
GAGGTGATATAGCAAACATG+TGG - chr8.4:22557899-22557918 MS.gene033403:intron 40.0%
GATTAGCATGAGCCACAATT+GGG - chr8.4:22557945-22557964 MS.gene033403:intron 40.0%
GGATTTCGATTCTCTTTCCA+AGG - chr8.4:22557832-22557851 MS.gene033403:intron 40.0%
GGTAGTTCATGCTTCAACTT+TGG - chr8.4:22557540-22557559 MS.gene033403:intron 40.0%
TGTCAAAATGTGAACGTGGT+GGG + chr8.4:22556974-22556993 None:intergenic 40.0%
TGTCTCATCGCCATAAAATG+TGG + chr8.4:22556306-22556325 None:intergenic 40.0%
TTGGTGTACGTGATGAATGT+GGG - chr8.4:22557871-22557890 MS.gene033403:intron 40.0%
! CACTAAGTGTTGCAAATGCT+TGG + chr8.4:22557981-22558000 None:intergenic 40.0%
! CCACTGTGCAATTTTCATGA+AGG + chr8.4:22557009-22557028 None:intergenic 40.0%
! CTTATTAAAGGAGGAGCATG+TGG - chr8.4:22556735-22556754 MS.gene033403:intron 40.0%
! CTTGCACATGCCACATTTTA+TGG - chr8.4:22556293-22556312 MS.gene033403:CDS 40.0%
! TGTGCAATTTTCATGAAGGC+AGG + chr8.4:22557005-22557024 None:intergenic 40.0%
! TTCTTAGAGTACCTTGCATG+AGG - chr8.4:22557803-22557822 MS.gene033403:intron 40.0%
AAAATTGCACAGTGGAAGGC+CGG - chr8.4:22557014-22557033 MS.gene033403:intron 45.0%
AATCCTTCACTCCTCATGCA+AGG + chr8.4:22557817-22557836 None:intergenic 45.0%
ATGCCTGAGACCAATTAGGA+GGG + chr8.4:22556929-22556948 None:intergenic 45.0%
ATGTGCAAGAGTCCATGAAC+TGG + chr8.4:22556282-22556301 None:intergenic 45.0%
GAATGTTGCTCCTTCCAACT+GGG - chr8.4:22558059-22558078 MS.gene033403:CDS 45.0%
GCACATTTCAACCCAGTTCA+TGG - chr8.4:22556267-22556286 MS.gene033403:CDS 45.0%
TGAAAGGAAGCAGAACAGGA+TGG - chr8.4:22557923-22557942 MS.gene033403:intron 45.0%
TGTGCAAGAGTCCATGAACT+GGG + chr8.4:22556281-22556300 None:intergenic 45.0%
! GGATTAGCATGAGCCACAAT+TGG - chr8.4:22557944-22557963 MS.gene033403:intron 45.0%
! TGCAAAGCCAGCTTTAGTAG+CGG - chr8.4:22556244-22556263 MS.gene033403:CDS 45.0%
!! AAAAATAAATAAAATAATGT+TGG + chr8.4:22556552-22556571 None:intergenic 5.0%
AATGTGCCCGCTACTAAAGC+TGG + chr8.4:22556254-22556273 None:intergenic 50.0%
GATGCCTGAGACCAATTAGG+AGG + chr8.4:22556930-22556949 None:intergenic 50.0%
GGAATGTTGCTCCTTCCAAC+TGG - chr8.4:22558058-22558077 MS.gene033403:CDS 50.0%
GTACCTTGCATGAGGAGTGA+AGG - chr8.4:22557811-22557830 MS.gene033403:intron 50.0%
GTGGCAGTGACTGTAGTGTA+AGG + chr8.4:22556897-22556916 None:intergenic 50.0%
GTGTACGTGATGAATGTGGG+AGG - chr8.4:22557874-22557893 MS.gene033403:intron 50.0%
GTTGCACCATCCACCGTAAT+CGG + chr8.4:22556952-22556971 None:intergenic 50.0%
TACGTGATGAATGTGGGAGG+TGG - chr8.4:22557877-22557896 MS.gene033403:intron 50.0%
TCGGATGCCTGAGACCAATT+AGG + chr8.4:22556933-22556952 None:intergenic 50.0%
TCGTCCCTGTTATGTATCGC+AGG - chr8.4:22557039-22557058 MS.gene033403:intron 50.0%
TGGGTGAAAGGAAGCAGAAC+AGG - chr8.4:22557919-22557938 MS.gene033403:intron 50.0%
TGGTCTCAGGCATCCGATTA+CGG - chr8.4:22556936-22556955 MS.gene033403:intron 50.0%
! AAAGGAGGAGCATGTGGGTA+TGG - chr8.4:22556741-22556760 MS.gene033403:intron 50.0%
! GCAAAGCCAGCTTTAGTAGC+GGG - chr8.4:22556245-22556264 MS.gene033403:CDS 50.0%
! TTGCCCTCCTAATTGGTCTC+AGG - chr8.4:22556923-22556942 MS.gene033403:intron 50.0%
!! GGTAAGAAGCACCCCAATTG+TGG + chr8.4:22557960-22557979 None:intergenic 50.0%
AATCCAGCAGCCCAGTTGGA+AGG + chr8.4:22558072-22558091 None:intergenic 55.0%
AGGCATCCGATTACGGTGGA+TGG - chr8.4:22556943-22556962 MS.gene033403:intron 55.0%
GAGACAGCTTCTGCCACCAT+GGG - chr8.4:22556320-22556339 MS.gene033403:CDS 55.0%
GGTTAATCCAGCAGCCCAGT+TGG + chr8.4:22558076-22558095 None:intergenic 55.0%
GTGATGAATGTGGGAGGTGG+AGG - chr8.4:22557880-22557899 MS.gene033403:intron 55.0%
TCTCAGGCATCCGATTACGG+TGG - chr8.4:22556939-22556958 MS.gene033403:intron 55.0%
TGAGACAGCTTCTGCCACCA+TGG - chr8.4:22556319-22556338 MS.gene033403:CDS 55.0%
GCTCCTTCCAACTGGGCTGC+TGG - chr8.4:22558066-22558085 MS.gene033403:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 22556176 22558122 22556176 ID=MS.gene033403
chr8.4 mRNA 22556176 22558122 22556176 ID=MS.gene033403.t1;Parent=MS.gene033403
chr8.4 exon 22557957 22558122 22557957 ID=MS.gene033403.t1.exon1;Parent=MS.gene033403.t1
chr8.4 CDS 22557957 22558122 22557957 ID=cds.MS.gene033403.t1;Parent=MS.gene033403.t1
chr8.4 exon 22557238 22557553 22557238 ID=MS.gene033403.t1.exon2;Parent=MS.gene033403.t1
chr8.4 CDS 22557238 22557553 22557238 ID=cds.MS.gene033403.t1;Parent=MS.gene033403.t1
chr8.4 exon 22556176 22556488 22556176 ID=MS.gene033403.t1.exon3;Parent=MS.gene033403.t1
chr8.4 CDS 22556176 22556488 22556176 ID=cds.MS.gene033403.t1;Parent=MS.gene033403.t1
Gene Sequence

>MS.gene033403

ATGGCTGCAATTTATCAATATTGTAGCTTCTTGGTTACAATTACAATTACACTGATGCTCACAATCATTGCAAAGCCAGCTTTAGTAGCGGGCACATTTCAACCCAGTTCATGGACTCTTGCACATGCCACATTTTATGGCGATGAGACAGCTTCTGCCACCATGGGTAAGTTATATCTCAATCTCTTAATTATTATCATTAGCTAAACCATTTCAGCAATTGATGTACAAGCCAAAATCAATCTCACTAAATTAACCTTTTTTGGTATAACACTATATTAACATTTTACTGACCAAACGATTGACGAACTATCATTTAAAACAAATTATTCAGAAGAACCCGAATTTGAATCCTACCTGAAATAATGTTTGACCAACATTATTTTATTTATTTTTTGACAAAGTTTAACATTATTGGAACTGCGGTTAAGACAAACATTTAACATCTTACTTAAATAGTTTGTAAATTGGTCATTAGCATTACCACAATTAATACTAATTTTCCCTTCTAGATATAAATAATTAAATAGTTTGTGGTAACTAATTAAAATTTGATGGACTTATTAAAGGAGGAGCATGTGGGTATGGAAATTTGTTTCTGAATGGTTATGGGACAGACACAGCAGCATTGAGTTCAACATTGTTCAATAATGGGTATGCATGTGGGACTTGTTATCAAATAAAATGTGTTCAATCAAGTGCATGTAACACCAATGTTCCTTACACTACAGTCACTGCCACAAATATTTGCCCTCCTAATTGGTCTCAGGCATCCGATTACGGTGGATGGTGCAACCCACCACGTTCACATTTTGACATGTCTAAGCCTGCCTTCATGAAAATTGCACAGTGGAAGGCCGGTATCGTCCCTGTTATGTATCGCAGGTAATTAACAAATCAAGCTTGCTCCACATATACATAATATAGAATAGATTAATAACAAAATGGTCGTCCATATTTTCTAATGTCTCATAAACATGCATATGATTTTGTCACGTGATCTATCTAGGCTATTGGATTAAAATCAGACGGTCCACATTTTAAATTTGGTAAATCAAATTTAAAGTACAAAGTGAAAATGTGATCTAATCTTGATTCAACATGATTGTGTGGATACATATATTTACAGTCACATGATTTTGACCATCATATCAAAATCAGACATTCCATGTTTCAAATTTGATAAATCGAGACATTCCATGTTTCAAATTTGATAAATCGAGTCCAAAAGTAGTTATTTGATCTTTATCTCGTAATCCAAATTGACATCTGCATGATTGCGTCGACATAAGAGTCCATATTTTCTATGGTCACAATGACACAATTGATGCAGTTATGTGATATAAACGGTTAAATCAAAGAGAGGTAGTTCATGCTTCAACTTTGGTAAATTGCATCTAAAATGCGAAGTTGAAATATGAATCATCCAATCTTGATACAATCTAGGTAGACAACAGCATGATTTTGTGATCACAAAGAATCCAAATTCAACCTTTTTTCTTGCATAATATGTTGCATCATTTGTCTTTTATGGAGATTATTCTTACCTTAACATTACAAAAGAGATATGTCTTTGGCTTTAGTGATATCGTACTTACAATGTGATTTGTTGATAAAAAAATTGAATTTCTTAGAGTACCTTGCATGAGGAGTGAAGGATTTCGATTCTCTTTCCAAGGAAATGGGTATTGGTTATTGGTGTACGTGATGAATGTGGGAGGTGGAGGTGATATAGCAAACATGTGGGTGAAAGGAAGCAGAACAGGATGGATTAGCATGAGCCACAATTGGGGTGCTTCTTACCAAGCATTTGCAACACTTAGTGGCCAAACTCTTTCTTTCAAGATTACTTCTTACACAACCAAAGAAACTATAATAGCTTGGAATGTTGCTCCTTCCAACTGGGCTGCTGGATTAACCTATTCCACACATGTCAATTTTCATTGA
Protein sequence

>MS.gene033403.t1

MAAIYQYCSFLVTITITLMLTIIAKPALVAGTFQPSSWTLAHATFYGDETASATMGGACGYGNLFLNGYGTDTAALSSTLFNNGYACGTCYQIKCVQSSACNTNVPYTTVTATNICPPNWSQASDYGGWCNPPRSHFDMSKPAFMKIAQWKAGIVPVMYRRVPCMRSEGFRFSFQGNGYWLLVYVMNVGGGGDIANMWVKGSRTGWISMSHNWGASYQAFATLSGQTLSFKITSYTTKETIIAWNVAPSNWAAGLTYSTHVNFH