AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004252

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004252.t1 MTR_2g016160 99.686 318 1 0 1 318 1 318 0.0 654
MS.gene004252.t1 MTR_4g073080 26.471 238 165 4 47 274 99 336 1.26e-19 89.0
MS.gene004252.t1 MTR_4g119620 26.047 215 154 5 96 309 67 277 2.75e-19 87.0
MS.gene004252.t1 MTR_4g119620 24.000 275 195 10 45 314 61 326 1.56e-12 67.8
MS.gene004252.t1 MTR_4g096880 23.411 299 187 6 54 310 105 403 1.48e-18 85.9
MS.gene004252.t1 MTR_4g096880 24.571 175 117 2 64 223 230 404 1.33e-11 65.5
MS.gene004252.t1 MTR_6g004040 27.315 216 149 6 51 266 49 256 3.24e-17 82.8
MS.gene004252.t1 MTR_2g012630 28.436 211 143 7 100 309 8 211 4.32e-17 82.4
MS.gene004252.t1 MTR_1g072140 29.412 187 126 5 125 309 86 268 8.41e-17 80.1
MS.gene004252.t1 MTR_7g104550 33.333 180 103 7 100 265 9 185 9.00e-17 80.1
MS.gene004252.t1 MTR_2g102277 30.000 190 125 6 78 265 73 256 2.16e-16 80.5
MS.gene004252.t1 MTR_2g102277 30.000 190 125 6 78 265 73 256 2.20e-16 80.5
MS.gene004252.t1 MTR_5g016350 29.670 182 113 7 97 269 408 583 2.38e-16 80.1
MS.gene004252.t1 MTR_5g016350 29.670 182 113 7 97 269 344 519 4.19e-16 79.0
MS.gene004252.t1 MTR_2g012630 28.436 211 143 7 100 309 8 211 7.67e-16 78.6
MS.gene004252.t1 MTR_4g011950 29.474 190 126 6 78 265 73 256 7.80e-16 78.6
MS.gene004252.t1 MTR_8g027985 25.843 267 172 10 60 310 649 905 2.38e-15 77.4
MS.gene004252.t1 MTR_4g095058 29.121 182 114 7 97 269 403 578 2.40e-15 77.0
MS.gene004252.t1 MTR_4g095058 29.121 182 114 7 97 269 401 576 2.55e-15 77.0
MS.gene004252.t1 MTR_8g080700 28.022 182 112 6 99 267 216 391 2.59e-15 76.6
MS.gene004252.t1 MTR_3g106080 28.700 223 126 9 72 267 358 574 3.41e-15 76.6
MS.gene004252.t1 MTR_5g097870 26.667 225 153 3 62 277 252 473 1.05e-14 74.7
MS.gene004252.t1 MTR_5g097870 27.811 169 115 1 106 267 250 418 1.92e-11 65.1
MS.gene004252.t1 MTR_1g050340 27.193 228 153 8 83 310 573 787 1.35e-14 74.7
MS.gene004252.t1 MTR_1g050340 27.193 228 153 8 83 310 572 786 1.45e-14 74.7
MS.gene004252.t1 MTR_5g016370 27.222 180 116 7 99 269 422 595 1.45e-14 74.7
MS.gene004252.t1 MTR_3g045130 25.664 226 159 7 84 309 294 510 2.19e-14 73.9
MS.gene004252.t1 MTR_3g045130 27.586 203 133 7 66 265 319 510 3.00e-14 73.6
MS.gene004252.t1 MTR_7g086360 29.452 146 99 4 122 267 112 253 3.49e-14 72.8
MS.gene004252.t1 MTR_5g009530 30.323 155 105 2 113 267 776 927 1.31e-13 72.0
MS.gene004252.t1 MTR_5g009530 30.323 155 105 2 113 267 776 927 1.47e-13 72.0
MS.gene004252.t1 MTR_5g022690 27.451 204 127 8 84 273 202 398 2.27e-13 70.9
MS.gene004252.t1 MTR_3g092840 25.962 312 199 11 3 292 18 319 9.28e-13 68.6
MS.gene004252.t1 MTR_2g085210 27.053 207 140 5 109 309 616 817 2.53e-12 68.2
MS.gene004252.t1 MTR_5g097870 27.835 194 128 3 62 246 252 442 3.96e-12 67.0
MS.gene004252.t1 MTR_5g097870 27.811 169 115 1 106 267 250 418 1.88e-11 65.1
MS.gene004252.t1 MTR_5g024090 30.319 188 105 9 99 269 210 388 4.87e-12 66.6
MS.gene004252.t1 MTR_4g105700 26.582 237 154 9 85 309 383 611 1.56e-11 65.5
MS.gene004252.t1 MTR_8g038550 25.472 212 146 8 55 265 221 421 9.10e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004252.t1 AT1G29260 81.191 319 57 2 1 318 1 317 0.0 558
MS.gene004252.t1 AT4G35050 26.383 235 160 4 46 267 101 335 3.84e-20 90.5
MS.gene004252.t1 AT5G58230 23.000 300 189 6 53 310 104 403 5.38e-20 90.1
MS.gene004252.t1 AT5G58230 24.000 175 118 2 64 223 230 404 1.21e-11 65.5
MS.gene004252.t1 AT3G49660 26.050 238 169 6 76 310 38 271 2.19e-19 87.4
MS.gene004252.t1 AT2G16780 23.729 236 167 3 45 267 99 334 3.09e-17 82.0
MS.gene004252.t1 AT2G16780 21.888 233 153 6 10 223 171 393 1.82e-11 65.1
MS.gene004252.t1 AT2G16780 22.872 188 139 3 40 223 149 334 9.64e-11 62.8
MS.gene004252.t1 AT5G08390 26.389 216 151 6 51 266 49 256 6.54e-17 82.0
MS.gene004252.t1 AT5G23430 26.389 216 151 6 51 266 49 256 8.62e-17 81.6
MS.gene004252.t1 AT5G23430 26.389 216 151 6 51 266 49 256 8.70e-17 81.6
MS.gene004252.t1 AT5G23430 26.389 216 151 6 51 266 49 256 8.70e-17 81.6
MS.gene004252.t1 AT2G26060 35.519 183 96 9 100 265 13 190 9.68e-17 80.1
MS.gene004252.t1 AT2G26060 35.519 183 96 9 100 265 13 190 1.25e-16 79.7
MS.gene004252.t1 AT2G05720 28.054 221 128 7 75 290 76 270 3.29e-16 77.8
MS.gene004252.t1 AT3G15980 30.000 190 125 6 78 265 73 256 3.34e-16 79.7
MS.gene004252.t1 AT3G15980 30.000 190 125 6 78 265 73 256 3.68e-16 79.7
MS.gene004252.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.27e-16 79.3
MS.gene004252.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.27e-16 79.3
MS.gene004252.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.27e-16 79.3
MS.gene004252.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.27e-16 79.3
MS.gene004252.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.30e-16 79.3
MS.gene004252.t1 AT1G52360 29.474 190 126 6 78 265 73 256 8.62e-16 78.6
MS.gene004252.t1 AT1G52360 29.474 190 126 6 78 265 73 256 8.62e-16 78.6
MS.gene004252.t1 AT1G52360 29.474 190 126 6 78 265 73 256 9.27e-16 78.6
MS.gene004252.t1 AT5G67320 29.670 182 113 7 97 269 438 613 9.69e-16 78.2
MS.gene004252.t1 AT1G52360 29.474 190 126 6 78 265 117 300 1.20e-15 78.2
MS.gene004252.t1 AT1G79990 30.000 190 125 6 78 265 73 256 1.85e-15 77.8
MS.gene004252.t1 AT1G79990 30.000 190 125 6 78 265 73 256 1.93e-15 77.4
MS.gene004252.t1 AT5G25150 28.626 262 154 11 72 309 361 613 3.96e-15 76.6
MS.gene004252.t1 AT5G25150 28.626 262 154 11 72 309 361 613 4.13e-15 76.3
MS.gene004252.t1 AT2G19540 27.374 179 117 4 99 267 215 390 8.62e-15 75.1
MS.gene004252.t1 AT1G61210 26.190 210 149 6 100 309 8 211 1.73e-14 74.7
MS.gene004252.t1 AT1G61210 25.500 200 140 6 71 269 67 258 1.17e-13 72.4
MS.gene004252.t1 AT1G61210 26.190 210 149 6 100 309 8 211 2.23e-14 74.3
MS.gene004252.t1 AT1G61210 25.500 200 140 6 71 269 67 258 1.66e-13 71.6
MS.gene004252.t1 AT1G61210 26.190 210 149 6 100 309 8 211 2.24e-14 74.3
MS.gene004252.t1 AT1G61210 25.500 200 140 6 71 269 67 258 1.87e-13 71.6
MS.gene004252.t1 AT4G18900 30.952 168 110 1 106 267 232 399 4.98e-14 72.8
MS.gene004252.t1 AT4G18900 27.803 223 148 5 64 277 236 454 5.46e-14 72.8
MS.gene004252.t1 AT2G43770 30.822 146 97 4 122 267 110 251 8.49e-14 71.6
MS.gene004252.t1 AT2G43770 27.570 214 136 9 62 266 97 300 4.30e-11 63.5
MS.gene004252.t1 AT1G11160 24.623 199 141 6 72 269 26 216 2.13e-13 71.2
MS.gene004252.t1 AT1G11160 24.623 199 141 6 72 269 68 258 2.28e-13 71.2
MS.gene004252.t1 AT1G11160 24.623 199 141 6 72 269 68 258 2.30e-13 71.2
MS.gene004252.t1 AT1G11160 24.623 199 141 6 72 269 68 258 2.40e-13 71.2
MS.gene004252.t1 AT2G32950 27.602 221 147 6 90 309 296 504 3.09e-13 70.5
MS.gene004252.t1 AT2G32950 27.602 221 147 6 90 309 409 617 4.18e-13 70.5
MS.gene004252.t1 AT4G11110 25.571 219 143 5 59 267 721 929 8.57e-13 69.7
MS.gene004252.t1 AT2G46340 27.184 206 135 5 113 309 771 970 1.44e-12 68.9
MS.gene004252.t1 AT2G46340 27.184 206 135 5 113 309 771 970 1.44e-12 68.9
MS.gene004252.t1 AT4G18905 26.754 228 151 5 62 277 276 499 1.88e-12 68.2
MS.gene004252.t1 AT4G02730 25.595 168 119 6 100 266 36 198 1.95e-12 67.4
MS.gene004252.t1 AT4G18905 26.754 228 151 5 62 277 266 489 2.08e-12 68.2
MS.gene004252.t1 AT4G35370 26.455 189 135 2 83 267 189 377 3.65e-12 67.0
MS.gene004252.t1 AT4G35370 27.461 193 134 3 64 253 219 408 1.50e-11 65.1
MS.gene004252.t1 AT5G24520 28.144 167 114 3 105 267 130 294 5.94e-12 66.2
MS.gene004252.t1 AT5G24520 28.144 167 114 3 105 267 130 294 5.94e-12 66.2
MS.gene004252.t1 AT5G24520 28.144 167 114 3 105 267 130 294 5.94e-12 66.2
MS.gene004252.t1 AT5G24520 28.144 167 114 3 105 267 130 294 5.94e-12 66.2
MS.gene004252.t1 AT3G16650 29.213 178 117 7 82 259 190 358 2.01e-11 65.1
MS.gene004252.t1 AT1G20540 26.087 184 128 5 87 265 100 280 2.78e-11 63.9
MS.gene004252.t1 AT3G15354 25.728 206 144 4 109 309 335 536 4.13e-11 64.3
MS.gene004252.t1 AT3G15354 25.728 206 144 4 109 309 335 536 4.13e-11 64.3
MS.gene004252.t1 AT3G15354 25.728 206 144 4 109 309 585 786 5.62e-11 63.9
MS.gene004252.t1 AT4G15900 24.561 228 161 7 82 309 196 412 6.93e-11 63.2
MS.gene004252.t1 AT5G13480 27.972 143 100 3 123 265 222 361 8.47e-11 63.2
MS.gene004252.t1 AT5G13480 27.972 143 100 3 123 265 210 349 8.55e-11 63.2
MS.gene004252.t1 AT5G13480 27.972 143 100 3 123 265 233 372 8.68e-11 63.2
MS.gene004252.t1 AT5G13480 27.972 143 100 3 123 265 210 349 9.02e-11 63.2

Find 94 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTGTACTGTCAGAATT+TGG 0.134510 2.4:-68855938 MS.gene004252:CDS
GTTCCGGTTGCCGTGCTTAA+TGG 0.204077 2.4:-68855011 MS.gene004252:CDS
GATTGGGAGGGCGGGGTTAG+AGG 0.268512 2.4:+68856316 None:intergenic
TTTGGGATGTAAGGAGTTAT+AGG 0.273478 2.4:-68855034 MS.gene004252:CDS
GGGACGATACCGTCAAATTA+TGG 0.291682 2.4:-68856067 MS.gene004252:CDS
TTCCGGTTGCCGTGCTTAAT+GGG 0.295179 2.4:-68855010 MS.gene004252:CDS
ATGATATGACTGTTTGTGTT+TGG 0.299620 2.4:-68854920 MS.gene004252:CDS
ATTGTGTTTATTCATCTGTT+TGG 0.320363 2.4:-68855998 MS.gene004252:CDS
TTGGGATGTAAGGAGTTATA+GGG 0.332484 2.4:-68855033 MS.gene004252:CDS
TGATATGACTGTTTGTGTTT+GGG 0.333313 2.4:-68854919 MS.gene004252:CDS
CAGTTAAGGTTTGGGATGTA+AGG 0.354149 2.4:-68855043 MS.gene004252:CDS
TTGATAAGTCAGTTAAGGTT+TGG 0.355478 2.4:-68855052 MS.gene004252:CDS
TGATAAGTCAGTTAAGGTTT+GGG 0.383380 2.4:-68855051 MS.gene004252:CDS
ATCATACTGAGTTTGCTGTT+GGG 0.389414 2.4:-68854857 MS.gene004252:CDS
TGTTCATGAAAGGAACGAAT+AGG 0.390721 2.4:+68856155 None:intergenic
GGGATGAACTTGTTTATGTT+TGG 0.393483 2.4:-68854788 MS.gene004252:CDS
GATGAATGTATAATTGCAAC+TGG 0.406242 2.4:-68855080 MS.gene004252:CDS
TGTAAGGAGTTATAGGGTTC+CGG 0.408160 2.4:-68855027 MS.gene004252:CDS
GCTTCTTGCTAGTACTGGTT+GGG 0.412462 2.4:-68854808 MS.gene004252:CDS
AGTTCGGTGATTGGGAGGGC+GGG 0.417786 2.4:+68856308 None:intergenic
ATGAGATTCTTTCGTGCGAT+TGG 0.420567 2.4:-68855112 MS.gene004252:CDS
GATATGAGTGTTCTTGTTGA+AGG 0.426691 2.4:-68854831 MS.gene004252:CDS
TGACGGAGTAACCGTTGAAT+GGG 0.426922 2.4:+68856432 None:intergenic
TGCCCATTAAGCACGGCAAC+CGG 0.427081 2.4:+68855008 None:intergenic
GTCGGTTGATAAGTCAGTTA+AGG 0.429070 2.4:-68855057 MS.gene004252:CDS
GAGTTCGGTGATTGGGAGGG+CGG 0.431125 2.4:+68856307 None:intergenic
GGCTTCTTGCTAGTACTGGT+TGG 0.432245 2.4:-68854809 MS.gene004252:CDS
AGAAACGAATCACGGCGAAC+AGG 0.432589 2.4:+68856101 None:intergenic
TTGACGGAGTAACCGTTGAA+TGG 0.442266 2.4:+68856431 None:intergenic
GGATTGTACTGTCAGAATTT+GGG 0.443764 2.4:-68855937 MS.gene004252:CDS
TAGGCGGCGAGTTCGGTGAT+TGG 0.444095 2.4:+68856299 None:intergenic
GAAGGGCTTCTTGCTAGTAC+TGG 0.444363 2.4:-68854813 MS.gene004252:CDS
GGTAATGGAGTCGTGAGATT+CGG 0.448864 2.4:+68856241 None:intergenic
AGGCGGCGAGTTCGGTGATT+GGG 0.454508 2.4:+68856300 None:intergenic
CGGGGTTAGAGGAGAGTTCA+AGG 0.456834 2.4:+68856327 None:intergenic
CTTGTTTATGTTTGGCAACA+TGG 0.461447 2.4:-68854780 MS.gene004252:CDS
GGTGTCGTAGGCGGCGAGTT+CGG 0.466638 2.4:+68856292 None:intergenic
GTTCATGAAAGGAACGAATA+GGG 0.469484 2.4:+68856156 None:intergenic
GTAGAAGGGGCTGAATTTGA+CGG 0.470246 2.4:+68856415 None:intergenic
CATCATACTGAGTTTGCTGT+TGG 0.478915 2.4:-68854858 MS.gene004252:CDS
GGCAACGGCGGCGACGGTAA+TGG 0.482055 2.4:+68856226 None:intergenic
ACCGTCGCCGCCGTTGCCGA+TGG 0.484087 2.4:-68856221 MS.gene004252:CDS
GTTCGGTGATTGGGAGGGCG+GGG 0.485951 2.4:+68856309 None:intergenic
ATTTGGGATGTTCGTGAACC+AGG 0.485985 2.4:-68855921 MS.gene004252:intron
TCATACTGAGTTTGCTGTTG+GGG 0.495869 2.4:-68854856 MS.gene004252:CDS
ATATGAGTGTTCTTGTTGAA+GGG 0.514929 2.4:-68854830 MS.gene004252:CDS
AGGTTCTACAATGATTCTTC+CGG 0.515402 2.4:-68855144 MS.gene004252:CDS
GCCGCATGTGAGGAATTTGT+TGG 0.515565 2.4:-68854955 MS.gene004252:CDS
GCATGGTTATGCTGTTAGAA+AGG 0.517131 2.4:-68854988 MS.gene004252:CDS
GATGCCGTCGGCGGTGTCGT+AGG 0.517224 2.4:+68856280 None:intergenic
GGCAACATGGAACTGATCCT+AGG 0.522032 2.4:-68854767 MS.gene004252:CDS
TTGAACGTTCTCACCGAAGT+GGG 0.523846 2.4:+68856032 None:intergenic
ACCATCGGCAACGGCGGCGA+CGG 0.530414 2.4:+68856220 None:intergenic
AGCGAGGTCGTAAAGTTTGA+CGG 0.535236 2.4:+68856196 None:intergenic
CGGCGAGTTCGGTGATTGGG+AGG 0.535883 2.4:+68856303 None:intergenic
CTTCCCTTTCAAGATGCCCT+AGG 0.540302 2.4:+68854750 None:intergenic
GTCGCCACCGCTCAAAACTT+CGG 0.541735 2.4:-68856377 MS.gene004252:CDS
GTTGCCGTGCTTAATGGGCA+TGG 0.541849 2.4:-68855005 MS.gene004252:CDS
ATCCACGGTCCATAATTTGA+CGG 0.542435 2.4:+68856058 None:intergenic
TAGAGGAGAGTTCAAGGACG+TGG 0.542958 2.4:+68856333 None:intergenic
AACTTCGGCATCCTCGGCAA+CGG 0.545359 2.4:-68856362 MS.gene004252:CDS
AGAATCTCATGTTCATGTGC+CGG 0.550681 2.4:+68855125 None:intergenic
TTCGTTTCTCTCCGCTGCGT+GGG 0.552872 2.4:-68856087 MS.gene004252:CDS
GCTCAAAACTTCGGCATCCT+CGG 0.554172 2.4:-68856368 MS.gene004252:CDS
CGAATAGGGTTTGAAGTAGG+TGG 0.559454 2.4:+68856170 None:intergenic
ATTCGTTTCTCTCCGCTGCG+TGG 0.561476 2.4:-68856088 MS.gene004252:CDS
CACCGAAGTGGGACGATCCA+CGG 0.563775 2.4:+68856043 None:intergenic
AGAGTTCAAGGACGTGGAGA+CGG 0.566645 2.4:+68856339 None:intergenic
GTAAAGTTTGACGGAACCAT+CGG 0.567165 2.4:+68856205 None:intergenic
GCAACATGGAACTGATCCTA+GGG 0.574620 2.4:-68854766 MS.gene004252:CDS
GGCGAGTTCGGTGATTGGGA+GGG 0.576026 2.4:+68856304 None:intergenic
CGTGGAGACGGCCGTTGCCG+AGG 0.577422 2.4:+68856351 None:intergenic
GACGGAACCATCGGCAACGG+CGG 0.578656 2.4:+68856214 None:intergenic
GAGCGGTGGCGACGGCGATG+CGG 0.585400 2.4:+68856387 None:intergenic
GCCGTCGGCGGTGTCGTAGG+CGG 0.589550 2.4:+68856283 None:intergenic
TTCACGTGTATGTTCATGAA+AGG 0.590355 2.4:+68856145 None:intergenic
GACCGTGGATCGTCCCACTT+CGG 0.593212 2.4:-68856045 MS.gene004252:CDS
TTTGAACGTTCTCACCGAAG+TGG 0.595208 2.4:+68856031 None:intergenic
GCCGCCTACGACACCGCCGA+CGG 0.600761 2.4:-68856284 MS.gene004252:CDS
GAACGAATAGGGTTTGAAGT+AGG 0.614418 2.4:+68856167 None:intergenic
TTTGACGGAACCATCGGCAA+CGG 0.622318 2.4:+68856211 None:intergenic
ACGGCATCTACGACGTCACA+TGG 0.628119 2.4:-68856265 MS.gene004252:CDS
ATAACCATGCCCATTAAGCA+CGG 0.633480 2.4:+68855001 None:intergenic
GACGGAGTAACCGTTGAATG+GGG 0.634585 2.4:+68856433 None:intergenic
ACCAACAAATTCCTCACATG+CGG 0.642131 2.4:+68854954 None:intergenic
GACGGTATCGTCCCACGCAG+CGG 0.642717 2.4:+68856076 None:intergenic
CAGCGGAGAGAAACGAATCA+CGG 0.652885 2.4:+68856093 None:intergenic
TACCGTCAAATTATGGACCG+TGG 0.654878 2.4:-68856060 MS.gene004252:CDS
TTGAAGTAGGTGGAAGAGCG+AGG 0.660138 2.4:+68856180 None:intergenic
GACGTCGTAGATGCCGTCGG+CGG 0.671920 2.4:+68856271 None:intergenic
TGTGACGTCGTAGATGCCGT+CGG 0.679756 2.4:+68856268 None:intergenic
ATGTATAATTGCAACTGGGT+CGG 0.683430 2.4:-68855075 MS.gene004252:CDS
GCGAACAGGATTATAATCAA+CGG 0.693874 2.4:+68856115 None:intergenic
ATGAATGTATAATTGCAACT+GGG 0.763887 2.4:-68855079 MS.gene004252:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAATATATTTTGACATTTT+TGG + chr2.4:68855880-68855899 None:intergenic 15.0%
!!! GATTTTTCTAAACAAATTTA+TGG + chr2.4:68855684-68855703 None:intergenic 15.0%
!! AATCCATTAAAGCAAAATTA+GGG - chr2.4:68855544-68855563 MS.gene004252:intron 20.0%
!! ATTACTTAAAAACCTAGTAT+TGG + chr2.4:68856001-68856020 None:intergenic 20.0%
!! TACTTAAATTAGTGTATGTT+TGG + chr2.4:68855942-68855961 None:intergenic 20.0%
!! TATATCAAAATTGTAATACC+TGG + chr2.4:68855307-68855326 None:intergenic 20.0%
!!! TTTTAGCTATAGTTAAGTTA+AGG - chr2.4:68855460-68855479 MS.gene004252:intron 20.0%
! ATCCATTAAAGCAAAATTAG+GGG - chr2.4:68855545-68855564 MS.gene004252:intron 25.0%
! ATGAATGTATAATTGCAACT+GGG - chr2.4:68856128-68856147 MS.gene004252:CDS 25.0%
! ATTGTGTTTATTCATCTGTT+TGG - chr2.4:68855209-68855228 MS.gene004252:intron 25.0%
! GAATCCATTAAAGCAAAATT+AGG - chr2.4:68855543-68855562 MS.gene004252:intron 25.0%
! TAACTATTACATCCAATACT+AGG - chr2.4:68855986-68856005 MS.gene004252:CDS 25.0%
! TATCTATTACATCTAATACC+AGG - chr2.4:68856043-68856062 MS.gene004252:CDS 25.0%
!! ATGGTTCAACTAACTTTTAA+GGG + chr2.4:68855665-68855684 None:intergenic 25.0%
!! TATGGTTCAACTAACTTTTA+AGG + chr2.4:68855666-68855685 None:intergenic 25.0%
!! TCTAAGTGTATGTTTCATTT+TGG - chr2.4:68855390-68855409 MS.gene004252:intron 25.0%
!!! AGAATTGATTTTGATAGGTT+TGG + chr2.4:68855787-68855806 None:intergenic 25.0%
AAGTAGAATCAGTGTATGTT+TGG + chr2.4:68855849-68855868 None:intergenic 30.0%
ATATGAGTGTTCTTGTTGAA+GGG - chr2.4:68856377-68856396 MS.gene004252:CDS 30.0%
ATGATATGACTGTTTGTGTT+TGG - chr2.4:68856287-68856306 MS.gene004252:CDS 30.0%
GATGAATGTATAATTGCAAC+TGG - chr2.4:68856127-68856146 MS.gene004252:CDS 30.0%
TGATAAGTCAGTTAAGGTTT+GGG - chr2.4:68856156-68856175 MS.gene004252:CDS 30.0%
TGATATGACTGTTTGTGTTT+GGG - chr2.4:68856288-68856307 MS.gene004252:CDS 30.0%
TGTAAAAATGAGCTGAACAT+CGG - chr2.4:68855501-68855520 MS.gene004252:intron 30.0%
TTGATAAGTCAGTTAAGGTT+TGG - chr2.4:68856155-68856174 MS.gene004252:CDS 30.0%
! TACCCCTAATTTTGCTTTAA+TGG + chr2.4:68855550-68855569 None:intergenic 30.0%
!! ATACACTGATTCTACTTTAG+AGG - chr2.4:68855852-68855871 MS.gene004252:intron 30.0%
!! ATACGGGTTAGAATTGATTA+TGG + chr2.4:68855816-68855835 None:intergenic 30.0%
!! GGGTTAGAATTGATTTTGAT+AGG + chr2.4:68855792-68855811 None:intergenic 30.0%
!! TGTTTGTGTTTGGGATTTTA+TGG - chr2.4:68856297-68856316 MS.gene004252:CDS 30.0%
!!! AATTTTGACTCTTCCAAAAG+TGG - chr2.4:68855914-68855933 MS.gene004252:intron 30.0%
AATTCTAACCCGTATAGAAG+TGG - chr2.4:68855821-68855840 MS.gene004252:intron 35.0%
AGGTTCTACAATGATTCTTC+CGG - chr2.4:68856063-68856082 MS.gene004252:CDS 35.0%
ATGTATAATTGCAACTGGGT+CGG - chr2.4:68856132-68856151 MS.gene004252:CDS 35.0%
CTTGTTTATGTTTGGCAACA+TGG - chr2.4:68856427-68856446 MS.gene004252:CDS 35.0%
GATATGAGTGTTCTTGTTGA+AGG - chr2.4:68856376-68856395 MS.gene004252:CDS 35.0%
GCGAACAGGATTATAATCAA+CGG + chr2.4:68855095-68855114 None:intergenic 35.0%
GCTTCAAGCAGAATCAATTA+TGG - chr2.4:68855733-68855752 MS.gene004252:intron 35.0%
GGATTGTACTGTCAGAATTT+GGG - chr2.4:68855270-68855289 MS.gene004252:intron 35.0%
GGGTTAGAATTGATTATGGA+GGG + chr2.4:68855812-68855831 None:intergenic 35.0%
GTTCATGAAAGGAACGAATA+GGG + chr2.4:68855054-68855073 None:intergenic 35.0%
TCAAGCAGAATCAATTATGG+AGG - chr2.4:68855736-68855755 MS.gene004252:intron 35.0%
TGTTCATGAAAGGAACGAAT+AGG + chr2.4:68855055-68855074 None:intergenic 35.0%
TTCACGTGTATGTTCATGAA+AGG + chr2.4:68855065-68855084 None:intergenic 35.0%
TTGGGATGTAAGGAGTTATA+GGG - chr2.4:68856174-68856193 MS.gene004252:CDS 35.0%
! GGGATGAACTTGTTTATGTT+TGG - chr2.4:68856419-68856438 MS.gene004252:CDS 35.0%
! GTTTTCAAAACCCCATTCAA+CGG - chr2.4:68854764-68854783 MS.gene004252:CDS 35.0%
! TGTGACTAAAAGTGAGTTGA+AGG - chr2.4:68855613-68855632 MS.gene004252:intron 35.0%
! TGTTTGGTTCCACTTCTATA+CGG + chr2.4:68855833-68855852 None:intergenic 35.0%
! TTTGGGATGTAAGGAGTTAT+AGG - chr2.4:68856173-68856192 MS.gene004252:CDS 35.0%
!! ATCATACTGAGTTTGCTGTT+GGG - chr2.4:68856350-68856369 MS.gene004252:CDS 35.0%
!! TGTATGTTTGGTTCCACTTT+TGG + chr2.4:68855930-68855949 None:intergenic 35.0%
!! TTGTGTTTGGGATTTTATGG+TGG - chr2.4:68856300-68856319 MS.gene004252:CDS 35.0%
!! TTTTATGGTGGAAGATGCTT+TGG - chr2.4:68856312-68856331 MS.gene004252:CDS 35.0%
!!! TTGAATGGGGTTTTGAAAAC+TGG + chr2.4:68854764-68854783 None:intergenic 35.0%
ACCAACAAATTCCTCACATG+CGG + chr2.4:68856256-68856275 None:intergenic 40.0%
AGAAGCCGATGCAAAAACAT+CGG + chr2.4:68855248-68855267 None:intergenic 40.0%
AGAATCTCATGTTCATGTGC+CGG + chr2.4:68856085-68856104 None:intergenic 40.0%
ATAACCATGCCCATTAAGCA+CGG + chr2.4:68856209-68856228 None:intergenic 40.0%
ATCCACGGTCCATAATTTGA+CGG + chr2.4:68855152-68855171 None:intergenic 40.0%
ATGAGATTCTTTCGTGCGAT+TGG - chr2.4:68856095-68856114 MS.gene004252:CDS 40.0%
GCATGGTTATGCTGTTAGAA+AGG - chr2.4:68856219-68856238 MS.gene004252:CDS 40.0%
GGAAGAATCATTGTAGAACC+TGG + chr2.4:68856064-68856083 None:intergenic 40.0%
GGGATTGTACTGTCAGAATT+TGG - chr2.4:68855269-68855288 MS.gene004252:intron 40.0%
GTAAAGTTTGACGGAACCAT+CGG + chr2.4:68855005-68855024 None:intergenic 40.0%
TGTAAGGAGTTATAGGGTTC+CGG - chr2.4:68856180-68856199 MS.gene004252:CDS 40.0%
! CAGTTAAGGTTTGGGATGTA+AGG - chr2.4:68856164-68856183 MS.gene004252:CDS 40.0%
! CGGGTTAGAATTGATTATGG+AGG + chr2.4:68855813-68855832 None:intergenic 40.0%
! GAACGAATAGGGTTTGAAGT+AGG + chr2.4:68855043-68855062 None:intergenic 40.0%
! GTTTGGTTCCACTTCTATAC+GGG + chr2.4:68855832-68855851 None:intergenic 40.0%
! TAGGGGTATGTTTGATTGTG+CGG - chr2.4:68855562-68855581 MS.gene004252:intron 40.0%
! TTAAATTTTCGCCGCATGTG+AGG - chr2.4:68856242-68856261 MS.gene004252:CDS 40.0%
!! CATCATACTGAGTTTGCTGT+TGG - chr2.4:68856349-68856368 MS.gene004252:CDS 40.0%
!! GTCGGTTGATAAGTCAGTTA+AGG - chr2.4:68856150-68856169 MS.gene004252:CDS 40.0%
!! TCATACTGAGTTTGCTGTTG+GGG - chr2.4:68856351-68856370 MS.gene004252:CDS 40.0%
!!! ATGTTTTTGCATCGGCTTCT+GGG - chr2.4:68855248-68855267 MS.gene004252:intron 40.0%
AGCGAGGTCGTAAAGTTTGA+CGG + chr2.4:68855014-68855033 None:intergenic 45.0%
GCAACATGGAACTGATCCTA+GGG - chr2.4:68856441-68856460 MS.gene004252:CDS 45.0%
GGGACGATACCGTCAAATTA+TGG - chr2.4:68855140-68855159 MS.gene004252:CDS 45.0%
GTAGAAGGGGCTGAATTTGA+CGG + chr2.4:68854795-68854814 None:intergenic 45.0%
TACCGTCAAATTATGGACCG+TGG - chr2.4:68855147-68855166 MS.gene004252:intron 45.0%
TGACGGAGTAACCGTTGAAT+GGG + chr2.4:68854778-68854797 None:intergenic 45.0%
TTGAACGTTCTCACCGAAGT+GGG + chr2.4:68855178-68855197 None:intergenic 45.0%
TTGACGGAGTAACCGTTGAA+TGG + chr2.4:68854779-68854798 None:intergenic 45.0%
TTTGAACGTTCTCACCGAAG+TGG + chr2.4:68855179-68855198 None:intergenic 45.0%
! ATTTGGGATGTTCGTGAACC+AGG - chr2.4:68855286-68855305 MS.gene004252:intron 45.0%
! CGAATAGGGTTTGAAGTAGG+TGG + chr2.4:68855040-68855059 None:intergenic 45.0%
!! GCTTCTTGCTAGTACTGGTT+GGG - chr2.4:68856399-68856418 MS.gene004252:CDS 45.0%
!! GGTAATGGAGTCGTGAGATT+CGG + chr2.4:68854969-68854988 None:intergenic 45.0%
!! TCACGCCGATGTTTTTGCAT+CGG - chr2.4:68855240-68855259 MS.gene004252:intron 45.0%
!!! GATGTTTTTGCATCGGCTTC+TGG - chr2.4:68855247-68855266 MS.gene004252:intron 45.0%
!!! TGTTTTTGCATCGGCTTCTG+GGG - chr2.4:68855249-68855268 MS.gene004252:intron 45.0%
AGAAACGAATCACGGCGAAC+AGG + chr2.4:68855109-68855128 None:intergenic 50.0%
AGAGTTCAAGGACGTGGAGA+CGG + chr2.4:68854871-68854890 None:intergenic 50.0%
CAGCGGAGAGAAACGAATCA+CGG + chr2.4:68855117-68855136 None:intergenic 50.0%
GAAGGGCTTCTTGCTAGTAC+TGG - chr2.4:68856394-68856413 MS.gene004252:CDS 50.0%
GACGGAGTAACCGTTGAATG+GGG + chr2.4:68854777-68854796 None:intergenic 50.0%
GCCGCATGTGAGGAATTTGT+TGG - chr2.4:68856252-68856271 MS.gene004252:CDS 50.0%
GCTCAAAACTTCGGCATCCT+CGG - chr2.4:68854839-68854858 MS.gene004252:CDS 50.0%
GGCAACATGGAACTGATCCT+AGG - chr2.4:68856440-68856459 MS.gene004252:CDS 50.0%
TAGAGGAGAGTTCAAGGACG+TGG + chr2.4:68854877-68854896 None:intergenic 50.0%
TTCCGGTTGCCGTGCTTAAT+GGG - chr2.4:68856197-68856216 MS.gene004252:CDS 50.0%
TTGAAGTAGGTGGAAGAGCG+AGG + chr2.4:68855030-68855049 None:intergenic 50.0%
TTTGACGGAACCATCGGCAA+CGG + chr2.4:68854999-68855018 None:intergenic 50.0%
! CGATGCGGTTTTCGTAGAAG+GGG + chr2.4:68854808-68854827 None:intergenic 50.0%
! GCGATGCGGTTTTCGTAGAA+GGG + chr2.4:68854809-68854828 None:intergenic 50.0%
!! GGCTTCTTGCTAGTACTGGT+TGG - chr2.4:68856398-68856417 MS.gene004252:CDS 50.0%
!!! GAGGATGCCGAAGTTTTGAG+CGG + chr2.4:68854840-68854859 None:intergenic 50.0%
AACTTCGGCATCCTCGGCAA+CGG - chr2.4:68854845-68854864 MS.gene004252:CDS 55.0%
ACGGCATCTACGACGTCACA+TGG - chr2.4:68854942-68854961 MS.gene004252:CDS 55.0%
ATTCGTTTCTCTCCGCTGCG+TGG - chr2.4:68855119-68855138 MS.gene004252:CDS 55.0%
CGGGGTTAGAGGAGAGTTCA+AGG + chr2.4:68854883-68854902 None:intergenic 55.0%
GCAAAAACATCGGCGTGACG+AGG + chr2.4:68855238-68855257 None:intergenic 55.0%
GTCGCCACCGCTCAAAACTT+CGG - chr2.4:68854830-68854849 MS.gene004252:CDS 55.0%
GTTCCGGTTGCCGTGCTTAA+TGG - chr2.4:68856196-68856215 MS.gene004252:CDS 55.0%
TGCCCATTAAGCACGGCAAC+CGG + chr2.4:68856202-68856221 None:intergenic 55.0%
TGTGACGTCGTAGATGCCGT+CGG + chr2.4:68854942-68854961 None:intergenic 55.0%
TTCGTTTCTCTCCGCTGCGT+GGG - chr2.4:68855120-68855139 MS.gene004252:CDS 55.0%
! GGCGATGCGGTTTTCGTAGA+AGG + chr2.4:68854810-68854829 None:intergenic 55.0%
! GTTGCCGTGCTTAATGGGCA+TGG - chr2.4:68856202-68856221 MS.gene004252:CDS 55.0%
!!! GAAGTTTTGAGCGGTGGCGA+CGG + chr2.4:68854831-68854850 None:intergenic 55.0%
!!! GATGCCGAAGTTTTGAGCGG+TGG + chr2.4:68854837-68854856 None:intergenic 55.0%
CACCGAAGTGGGACGATCCA+CGG + chr2.4:68855167-68855186 None:intergenic 60.0%
GACCGTGGATCGTCCCACTT+CGG - chr2.4:68855162-68855181 MS.gene004252:intron 60.0%
! AGGCGGCGAGTTCGGTGATT+GGG + chr2.4:68854910-68854929 None:intergenic 60.0%
! AGTTCGGTGATTGGGAGGGC+GGG + chr2.4:68854902-68854921 None:intergenic 60.0%
! GAGTTCGGTGATTGGGAGGG+CGG + chr2.4:68854903-68854922 None:intergenic 60.0%
! GGCGAGTTCGGTGATTGGGA+GGG + chr2.4:68854906-68854925 None:intergenic 60.0%
! TAGGCGGCGAGTTCGGTGAT+TGG + chr2.4:68854911-68854930 None:intergenic 60.0%
GACGGAACCATCGGCAACGG+CGG + chr2.4:68854996-68855015 None:intergenic 65.0%
GACGGTATCGTCCCACGCAG+CGG + chr2.4:68855134-68855153 None:intergenic 65.0%
GACGTCGTAGATGCCGTCGG+CGG + chr2.4:68854939-68854958 None:intergenic 65.0%
GATTGGGAGGGCGGGGTTAG+AGG + chr2.4:68854894-68854913 None:intergenic 65.0%
! CGGCGAGTTCGGTGATTGGG+AGG + chr2.4:68854907-68854926 None:intergenic 65.0%
! GGTGTCGTAGGCGGCGAGTT+CGG + chr2.4:68854918-68854937 None:intergenic 65.0%
! GTTCGGTGATTGGGAGGGCG+GGG + chr2.4:68854901-68854920 None:intergenic 65.0%
ACCATCGGCAACGGCGGCGA+CGG + chr2.4:68854990-68855009 None:intergenic 70.0%
GGCAACGGCGGCGACGGTAA+TGG + chr2.4:68854984-68855003 None:intergenic 70.0%
!! GATGCCGTCGGCGGTGTCGT+AGG + chr2.4:68854930-68854949 None:intergenic 70.0%
ACCGTCGCCGCCGTTGCCGA+TGG - chr2.4:68854986-68855005 MS.gene004252:CDS 75.0%
CGTGGAGACGGCCGTTGCCG+AGG + chr2.4:68854859-68854878 None:intergenic 75.0%
GAGCGGTGGCGACGGCGATG+CGG + chr2.4:68854823-68854842 None:intergenic 75.0%
GCCGCCTACGACACCGCCGA+CGG - chr2.4:68854923-68854942 MS.gene004252:CDS 75.0%
!! GCCGTCGGCGGTGTCGTAGG+CGG + chr2.4:68854927-68854946 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 68854758 68856471 68854758 ID=MS.gene004252
chr2.4 mRNA 68854758 68856471 68854758 ID=MS.gene004252.t1;Parent=MS.gene004252
chr2.4 exon 68855922 68856471 68855922 ID=MS.gene004252.t1.exon1;Parent=MS.gene004252.t1
chr2.4 CDS 68855922 68856471 68855922 ID=cds.MS.gene004252.t1;Parent=MS.gene004252.t1
chr2.4 exon 68854758 68855164 68854758 ID=MS.gene004252.t1.exon2;Parent=MS.gene004252.t1
chr2.4 CDS 68854758 68855164 68854758 ID=cds.MS.gene004252.t1;Parent=MS.gene004252.t1
Gene Sequence

>MS.gene004252

ATGCCAGTTTTCAAAACCCCATTCAACGGTTACTCCGTCAAATTCAGCCCCTTCTACGAAAACCGCATCGCCGTCGCCACCGCTCAAAACTTCGGCATCCTCGGCAACGGCCGTCTCCACGTCCTTGAACTCTCCTCTAACCCCGCCCTCCCAATCACCGAACTCGCCGCCTACGACACCGCCGACGGCATCTACGACGTCACATGGTCCGAATCTCACGACTCCATTACCGTCGCCGCCGTTGCCGATGGTTCCGTCAAACTTTACGACCTCGCTCTTCCACCTACTTCAAACCCTATTCGTTCCTTTCATGAACATACACGTGAAGTTCATTCCGTTGATTATAATCCTGTTCGCCGTGATTCGTTTCTCTCCGCTGCGTGGGACGATACCGTCAAATTATGGACCGTGGATCGTCCCACTTCGGTGAGAACGTTCAAAGAGCATGCTTATTGTGTTTATTCATCTGTTTGGAATCCTCGTCACGCCGATGTTTTTGCATCGGCTTCTGGGGATTGTACTGTCAGAATTTGGGATGTTCGTGAACCAGGTATTACAATTTTGATATAACTCACAAATAAATAACAAATTATACAATGATGCTTATATAGCAAATTAAAACAATTGATTAGTCTAAGTGTATGTTTCATTTTGGAGTGAGAAAAATTGATTTTGTTTAGTAAATTGATTCTGTGAAATTGATTTTAGCTATAGTTAAGTTAAGGTAAAGTGATTTATGTTCATGTAAAAATGAGCTGAACATCGGCAATTTCTAGCTTCAAGTAGAATCCATTAAAGCAAAATTAGGGGTATGTTTGATTGTGCGGTGACAAGAATTGATTTAGTAAAATTGATTGTGACTAAAAGTGAGTTGAAGGTAAAGTGATTTGTGTTTGAATACATTCCCTTAAAAGTTAGTTGAACCATAAATTTGTTTAGAAAAATCAATTCTAGAATCAAAAGCTACAAGTTCTAGCTTCAAGCAGAATCAATTATGGAGGCAAAATCAATAATACTCGTAAGCAACCAAACCTATCAAAATCAATTCTAACCCTCCATAATCAATTCTAACCCGTATAGAAGTGGAACCAAACATACACTGATTCTACTTTAGAGGAACCAAAAATGTCAAAATATATTCTACACTTGTAGAATCAATTTTGACTCTTCCAAAAGTGGAACCAAACATACACTAATTTAAGTAACAATTGTGACTAATTATTCTAACTAACTATTACATCCAATACTAGGTTTTTAAGTAATCAGTGTGACTAATTATTCTAACTATCTATTACATCTAATACCAGGTTCTACAATGATTCTTCCGGCACATGAACATGAGATTCTTTCGTGCGATTGGAATAAGTATGATGAATGTATAATTGCAACTGGGTCGGTTGATAAGTCAGTTAAGGTTTGGGATGTAAGGAGTTATAGGGTTCCGGTTGCCGTGCTTAATGGGCATGGTTATGCTGTTAGAAAGGTTAAATTTTCGCCGCATGTGAGGAATTTGTTGGTGTCTTGTTCTTATGATATGACTGTTTGTGTTTGGGATTTTATGGTGGAAGATGCTTTGGTGAGTAGATATGATCATCATACTGAGTTTGCTGTTGGGGTTGATATGAGTGTTCTTGTTGAAGGGCTTCTTGCTAGTACTGGTTGGGATGAACTTGTTTATGTTTGGCAACATGGAACTGATCCTAGGGCATCTTGA
Protein sequence

>MS.gene004252.t1

MPVFKTPFNGYSVKFSPFYENRIAVATAQNFGILGNGRLHVLELSSNPALPITELAAYDTADGIYDVTWSESHDSITVAAVADGSVKLYDLALPPTSNPIRSFHEHTREVHSVDYNPVRRDSFLSAAWDDTVKLWTVDRPTSVRTFKEHAYCVYSSVWNPRHADVFASASGDCTVRIWDVREPGSTMILPAHEHEILSCDWNKYDECIIATGSVDKSVKVWDVRSYRVPVAVLNGHGYAVRKVKFSPHVRNLLVSCSYDMTVCVWDFMVEDALVSRYDHHTEFAVGVDMSVLVEGLLASTGWDELVYVWQHGTDPRAS