AlfalfaGEDB | MS.gene004813

M. sativa cultivar XinJiangDaYe / MS.gene004813

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0006950 GO:0008150 GO:0009611 GO:0030234 GO:0030414 GO:0050896 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00280.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004813.t1	XP_003590795.1	88.6	70	8	0	1	70	1	70	1.50E-28	134.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004813.t1	P19873	62.1	66	25	0	5	70	3	68	6.1e-18	90.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004813.t1	G7I3Z2	88.6	70	8	0	1	70	1	70	1.1e-28	134.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene004813	MS.gene39886	PPI
MS.gene004813	MS.gene52993	PPI
MS.gene004813	MS.gene036131	PPI
MS.gene004813	MS.gene038231	PPI
MS.gene004813	MS.gene47682	PPI
MS.gene004813	MS.gene71446	PPI
MS.gene004813	MS.gene017022	PPI
MS.gene004813	MS.gene038093	PPI
MS.gene004813	MS.gene47590	PPI
MS.gene004813	MS.gene49324	PPI
MS.gene004813	MS.gene066013	PPI
MS.gene004813	MS.gene017021	PPI
MS.gene39886	MS.gene004813	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004813.t1	MTR_1g075340	88.571	70	8	1	70	1	70	8.80e-41	128
MS.gene004813.t1	MTR_1g075340	88.571	70	8	1	70	1	70	8.80e-41	128
MS.gene004813.t1	MTR_1g075410	83.824	68	11	3	70	5	72	1.22e-35	115
MS.gene004813.t1	MTR_5g090250	82.857	70	12	1	70	1	70	5.02e-35	113
MS.gene004813.t1	MTR_5g090250	82.857	70	12	1	70	1	70	5.02e-35	113
MS.gene004813.t1	MTR_1g075380	80.882	68	13	3	70	4	71	1.59e-32	107
MS.gene004813.t1	MTR_1g070335	55.714	70	31	1	70	1	70	1.49e-22	82.0
MS.gene004813.t1	MTR_5g045470	55.224	67	30	4	70	3	69	1.22e-19	74.7
MS.gene004813.t1	MTR_1g070330	55.556	54	24	17	70	1	54	9.54e-15	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004813.t1	AT2G38870	52.857	70	33	0	1	70	1	70	1.62e-21	79.3
MS.gene004813.t1	AT5G43580	55.556	72	30	1	1	70	28	99	3.31e-21	79.7
MS.gene004813.t1	AT3G46860	46.032	63	34	0	8	70	23	85	1.39e-13	59.7
MS.gene004813.t1	AT5G43570	47.945	73	32	1	1	67	1	73	9.30e-13	58.2
MS.gene004813.t1	AT5G43570	51.667	60	29	0	8	67	24	83	9.54e-12	55.8
MS.gene004813.t1	AT2G38900	42.188	64	37	0	7	70	10	73	1.18e-11	54.7
MS.gene004813.t1	AT2G38900	42.188	64	37	0	7	70	25	88	1.65e-11	54.7

Find 26 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGTGACAGGGTTTGGGTTT+GGG	0.259063	1.4:+61737019	MS.gene004813:CDS
ACTTCCGCTGTGACAGGGTT+TGG	0.267379	1.4:+61737012	MS.gene004813:CDS
CTTCCGCTGTGACAGGGTTT+GGG	0.309008	1.4:+61737013	MS.gene004813:CDS
GCTGTGACAGGGTTTGGGTT+TGG	0.326505	1.4:+61737018	MS.gene004813:CDS
CCTTTGACTTCCGCTGTGAC+AGG	0.333094	1.4:+61737006	MS.gene004813:CDS
TGGGTTTGGGTTAATAAAGA+TGG	0.418377	1.4:+61737032	MS.gene004813:CDS
CCTTCCACTCCAACCAACTC+TGG	0.454716	1.4:-61736906	None:intergenic
CTTTGACTTCCGCTGTGACA+GGG	0.463269	1.4:+61737007	MS.gene004813:CDS
CTGTCACAGCGGAAGTCAAA+GGG	0.467050	1.4:-61737005	None:intergenic
TGTGCTCAGGTAAGAGTTCA+TGG	0.479878	1.4:+61736883	MS.gene004813:intron
GATGCCATCATTGTGCTAGA+AGG	0.504821	1.4:+61736972	MS.gene004813:CDS
CCAGAGTTGGTTGGAGTGGA+AGG	0.515293	1.4:+61736906	MS.gene004813:CDS
GTTGGTTGGAGTGGAAGGAA+AGG	0.527079	1.4:+61736911	MS.gene004813:CDS
AGTTCCTTCTAGCACAATGA+TGG	0.538007	1.4:-61736976	None:intergenic
AATTCAGAGGGAGAATCCAT+TGG	0.542280	1.4:+61736947	MS.gene004813:CDS
ATGGCCAGAGTTGGTTGGAG+TGG	0.545068	1.4:+61736902	MS.gene004813:CDS
CCTGTCACAGCGGAAGTCAA+AGG	0.545493	1.4:-61737006	None:intergenic
TAAGAGTTCATGGCCAGAGT+TGG	0.563662	1.4:+61736893	MS.gene004813:CDS
AGTTCATGGCCAGAGTTGGT+TGG	0.565712	1.4:+61736897	MS.gene004813:CDS
TAAAGATGGAATTGTCACTC+AGG	0.589757	1.4:+61737046	MS.gene004813:CDS
AGCAGAAGCTACAATTCAGA+GGG	0.593359	1.4:+61736935	MS.gene004813:CDS
TAGCAGAAGCTACAATTCAG+AGG	0.596029	1.4:+61736934	MS.gene004813:CDS
ACAATGATGGCATCAACCAA+TGG	0.650325	1.4:-61736963	None:intergenic
AAAATGTCTGATGAATGCAA+AGG	0.675933	1.4:+61736762	None:intergenic
TGTCACAGCGGAAGTCAAAG+GGG	0.698463	1.4:-61737004	None:intergenic
AAACCCAAACCCTGTCACAG+CGG	0.730650	1.4:-61737016	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAAAGATGGAATTGTCACTC+AGG	+	chr1.4:61737046-61737065	MS.gene004813:CDS	35.0%
	TCAATATATTGTGTGTGCTC+AGG	+	chr1.4:61736870-61736889	MS.gene004813:intron	35.0%
!	TGGGTTTGGGTTAATAAAGA+TGG	+	chr1.4:61737032-61737051	MS.gene004813:CDS	35.0%
	AATTCAGAGGGAGAATCCAT+TGG	+	chr1.4:61736947-61736966	MS.gene004813:CDS	40.0%
	ACAATGATGGCATCAACCAA+TGG	-	chr1.4:61736966-61736985	None:intergenic	40.0%
	AGCAGAAGCTACAATTCAGA+GGG	+	chr1.4:61736935-61736954	MS.gene004813:CDS	40.0%
	AGTTCCTTCTAGCACAATGA+TGG	-	chr1.4:61736979-61736998	None:intergenic	40.0%
	GTCACTCAGGTTCCAAAAAT+TGG	+	chr1.4:61737059-61737078	MS.gene004813:CDS	40.0%
	GTCTGATGAATGCAAAGGTA+AGG	+	chr1.4:61736767-61736786	MS.gene004813:intron	40.0%
	TAGCAGAAGCTACAATTCAG+AGG	+	chr1.4:61736934-61736953	MS.gene004813:CDS	40.0%
	GATGCCATCATTGTGCTAGA+AGG	+	chr1.4:61736972-61736991	MS.gene004813:CDS	45.0%
	TAAGAGTTCATGGCCAGAGT+TGG	+	chr1.4:61736893-61736912	MS.gene004813:CDS	45.0%
	TGTGCTCAGGTAAGAGTTCA+TGG	+	chr1.4:61736883-61736902	MS.gene004813:intron	45.0%
!!!	AAATTTTAATTATAATTTGA+TGG	+	chr1.4:61736838-61736857	MS.gene004813:intron	5.0%
	AAACCCAAACCCTGTCACAG+CGG	-	chr1.4:61737019-61737038	None:intergenic	50.0%
	CTGTCACAGCGGAAGTCAAA+GGG	-	chr1.4:61737008-61737027	None:intergenic	50.0%
	GTTGGTTGGAGTGGAAGGAA+AGG	+	chr1.4:61736911-61736930	MS.gene004813:CDS	50.0%
	TGTCACAGCGGAAGTCAAAG+GGG	-	chr1.4:61737007-61737026	None:intergenic	50.0%
!	AGTTCATGGCCAGAGTTGGT+TGG	+	chr1.4:61736897-61736916	MS.gene004813:CDS	50.0%
!	CTGTGACAGGGTTTGGGTTT+GGG	+	chr1.4:61737019-61737038	MS.gene004813:CDS	50.0%
!	CTTTGACTTCCGCTGTGACA+GGG	+	chr1.4:61737007-61737026	MS.gene004813:CDS	50.0%
	ACTTCCGCTGTGACAGGGTT+TGG	+	chr1.4:61737012-61737031	MS.gene004813:CDS	55.0%
	CCTGTCACAGCGGAAGTCAA+AGG	-	chr1.4:61737009-61737028	None:intergenic	55.0%
	CCTTCCACTCCAACCAACTC+TGG	-	chr1.4:61736909-61736928	None:intergenic	55.0%
	CTTCCGCTGTGACAGGGTTT+GGG	+	chr1.4:61737013-61737032	MS.gene004813:CDS	55.0%
!	ATGGCCAGAGTTGGTTGGAG+TGG	+	chr1.4:61736902-61736921	MS.gene004813:CDS	55.0%
!	CCAGAGTTGGTTGGAGTGGA+AGG	+	chr1.4:61736906-61736925	MS.gene004813:CDS	55.0%
!	CCTTTGACTTCCGCTGTGAC+AGG	+	chr1.4:61737006-61737025	MS.gene004813:CDS	55.0%
!	GCTGTGACAGGGTTTGGGTT+TGG	+	chr1.4:61737018-61737037	MS.gene004813:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	61736765	61737085	61736765	ID=MS.gene004813
chr1.4	mRNA	61736765	61737085	61736765	ID=MS.gene004813.t1;Parent=MS.gene004813
chr1.4	exon	61736765	61736783	61736765	ID=MS.gene004813.t1.exon1;Parent=MS.gene004813.t1
chr1.4	CDS	61736765	61736783	61736765	ID=cds.MS.gene004813.t1;Parent=MS.gene004813.t1
chr1.4	exon	61736892	61737085	61736892	ID=MS.gene004813.t1.exon2;Parent=MS.gene004813.t1
chr1.4	CDS	61736892	61737085	61736892	ID=cds.MS.gene004813.t1;Parent=MS.gene004813.t1

Gene Sequence	>MS.gene004813 ATGTCTGATGAATGCAAAGGTAAGGTGTTATAATATAATATGTTTACATGCATACATATGAATAATAAATTAAAAATTTTAATTATAATTTGATGGTTGAATGAATCAATATATTGTGTGTGCTCAGGTAAGAGTTCATGGCCAGAGTTGGTTGGAGTGGAAGGAAAGGTAGCAGAAGCTACAATTCAGAGGGAGAATCCATTGGTTGATGCCATCATTGTGCTAGAAGGAACTGATGTCCCCTTTGACTTCCGCTGTGACAGGGTTTGGGTTTGGGTTAATAAAGATGGAATTGTCACTCAGGTTCCAAAAATTGGATAG
Protein sequence	>MS.gene004813.t1 MSDECKGKSSWPELVGVEGKVAEATIQRENPLVDAIIVLEGTDVPFDFRCDRVWVWVNKDGIVTQVPKIG