Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47682.t1 | AET00273.1 | 94.3 | 70 | 4 | 0 | 1 | 70 | 1 | 70 | 7.40E-31 | 142.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47682.t1 | P24076 | 65.7 | 67 | 23 | 0 | 4 | 70 | 2 | 68 | 1.6e-18 | 92.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47682.t1 | G7KCZ8 | 94.3 | 70 | 4 | 0 | 1 | 70 | 1 | 70 | 5.3e-31 | 142.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052089 | MS.gene47682 | 0.804006 | 2.66E-49 | -1.69E-46 |
| MS.gene055549 | MS.gene47682 | 0.814366 | 1.60E-51 | -1.69E-46 |
| MS.gene055550 | MS.gene47682 | 0.822043 | 2.92E-53 | -1.69E-46 |
| MS.gene056530 | MS.gene47682 | 0.815447 | 9.20E-52 | -1.69E-46 |
| MS.gene058493 | MS.gene47682 | 0.815152 | 1.07E-51 | -1.69E-46 |
| MS.gene058494 | MS.gene47682 | 0.808418 | 3.13E-50 | -1.69E-46 |
| MS.gene05884 | MS.gene47682 | 0.828862 | 7.10E-55 | -1.69E-46 |
| MS.gene059514 | MS.gene47682 | 0.814417 | 1.56E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038231 | MS.gene47682 | PPI |
| MS.gene038093 | MS.gene47682 | PPI |
| MS.gene47590 | MS.gene47682 | PPI |
| MS.gene71446 | MS.gene47682 | PPI |
| MS.gene017021 | MS.gene47682 | PPI |
| MS.gene49324 | MS.gene47682 | PPI |
| MS.gene004813 | MS.gene47682 | PPI |
| MS.gene52993 | MS.gene47682 | PPI |
| MS.gene066013 | MS.gene47682 | PPI |
| MS.gene036131 | MS.gene47682 | PPI |
| MS.gene017022 | MS.gene47682 | PPI |
| MS.gene39886 | MS.gene47682 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47682.t1 | MTR_5g090250 | 94.286 | 70 | 4 | 0 | 1 | 70 | 1 | 70 | 1.62e-44 | 137 |
| MS.gene47682.t1 | MTR_5g090250 | 94.286 | 70 | 4 | 0 | 1 | 70 | 1 | 70 | 1.62e-44 | 137 |
| MS.gene47682.t1 | MTR_1g075410 | 89.706 | 68 | 7 | 0 | 3 | 70 | 5 | 72 | 1.02e-38 | 122 |
| MS.gene47682.t1 | MTR_1g075340 | 84.286 | 70 | 11 | 0 | 1 | 70 | 1 | 70 | 7.97e-38 | 120 |
| MS.gene47682.t1 | MTR_1g075340 | 84.286 | 70 | 11 | 0 | 1 | 70 | 1 | 70 | 7.97e-38 | 120 |
| MS.gene47682.t1 | MTR_1g075380 | 82.353 | 68 | 12 | 0 | 3 | 70 | 4 | 71 | 1.33e-33 | 110 |
| MS.gene47682.t1 | MTR_1g070335 | 60.000 | 70 | 28 | 0 | 1 | 70 | 1 | 70 | 8.17e-24 | 85.1 |
| MS.gene47682.t1 | MTR_5g045470 | 62.121 | 66 | 25 | 0 | 5 | 70 | 4 | 69 | 9.91e-24 | 85.1 |
| MS.gene47682.t1 | MTR_1g070330 | 59.259 | 54 | 22 | 0 | 17 | 70 | 1 | 54 | 1.31e-15 | 63.9 |
| MS.gene47682.t1 | MTR_3g020970 | 50.794 | 63 | 31 | 0 | 8 | 70 | 36 | 98 | 2.08e-14 | 62.4 |
| MS.gene47682.t1 | MTR_3g020930 | 42.857 | 63 | 36 | 0 | 8 | 70 | 32 | 94 | 3.43e-11 | 53.9 |
| MS.gene47682.t1 | MTR_3g020800 | 47.619 | 63 | 33 | 0 | 8 | 70 | 36 | 98 | 4.98e-11 | 53.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47682.t1 | AT2G38870 | 60.000 | 70 | 28 | 0 | 1 | 70 | 1 | 70 | 5.34e-24 | 85.9 |
| MS.gene47682.t1 | AT5G43580 | 54.688 | 64 | 29 | 0 | 7 | 70 | 36 | 99 | 6.50e-19 | 73.6 |
| MS.gene47682.t1 | AT2G38900 | 48.438 | 64 | 33 | 0 | 7 | 70 | 10 | 73 | 1.28e-16 | 67.0 |
| MS.gene47682.t1 | AT2G38900 | 48.438 | 64 | 33 | 0 | 7 | 70 | 25 | 88 | 1.91e-16 | 67.0 |
| MS.gene47682.t1 | AT3G46860 | 47.619 | 63 | 33 | 0 | 8 | 70 | 23 | 85 | 1.50e-13 | 59.7 |
| MS.gene47682.t1 | AT5G43570 | 45.946 | 74 | 34 | 1 | 1 | 68 | 1 | 74 | 4.49e-13 | 58.9 |
| MS.gene47682.t1 | AT5G43570 | 49.180 | 61 | 31 | 0 | 8 | 68 | 24 | 84 | 4.42e-12 | 56.6 |
| MS.gene47682.t1 | AT5G45277 | 47.170 | 53 | 28 | 0 | 8 | 60 | 2 | 54 | 7.58e-11 | 52.4 |
Find 28 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTATCAGGTTCCAGTAAT+TGG | 0.190800 | 5.3:-72789173 | MS.gene47682:CDS |
| CTGTGACAGGGTTAGGGTTT+GGG | 0.262714 | 5.3:-72789213 | MS.gene47682:CDS |
| GACACTTTCTATCCAATTAC+TGG | 0.267846 | 5.3:+72789161 | None:intergenic |
| ACTTCCGCTGTGACAGGGTT+AGG | 0.276358 | 5.3:-72789220 | MS.gene47682:CDS |
| TAAAGATGGAATTGTCTATC+AGG | 0.346883 | 5.3:-72789186 | MS.gene47682:CDS |
| GCTGTGACAGGGTTAGGGTT+TGG | 0.358066 | 5.3:-72789214 | MS.gene47682:CDS |
| AGGGTTTGGGTTGATAAAGA+TGG | 0.412719 | 5.3:-72789200 | MS.gene47682:CDS |
| CCGCCGACTTCCGCTGTGAC+AGG | 0.421191 | 5.3:-72789226 | MS.gene47682:CDS |
| CTTCCGCTGTGACAGGGTTA+GGG | 0.422816 | 5.3:-72789219 | MS.gene47682:CDS |
| CCTTCCACTCCAACCAACTC+TGG | 0.454716 | 5.3:+72789326 | None:intergenic |
| AGATCCTTCTGGCACAATGA+TGG | 0.502286 | 5.3:+72789256 | None:intergenic |
| CCAGAGTTGGTTGGAGTGGA+AGG | 0.515293 | 5.3:-72789326 | MS.gene47682:CDS |
| GTTGGTTGGAGTGGAAGGAA+AGG | 0.527079 | 5.3:-72789321 | MS.gene47682:CDS |
| GCGGTGACAAAAGATCCTTC+TGG | 0.550057 | 5.3:+72789245 | None:intergenic |
| TATGGTCAGGTAAGAGCTCA+TGG | 0.552544 | 5.3:-72789349 | MS.gene47682:intron |
| CGCCGACTTCCGCTGTGACA+GGG | 0.553611 | 5.3:-72789225 | MS.gene47682:CDS |
| ATGGCCAGAGTTGGTTGGAG+TGG | 0.564909 | 5.3:-72789330 | MS.gene47682:CDS |
| AATTGAGAGGGAGAATCCAT+TGG | 0.570404 | 5.3:-72789285 | MS.gene47682:CDS |
| AACCCTGTCACAGCGGAAGT+CGG | 0.575336 | 5.3:+72789223 | None:intergenic |
| AGCTCATGGCCAGAGTTGGT+TGG | 0.578782 | 5.3:-72789335 | MS.gene47682:CDS |
| TAAGAGCTCATGGCCAGAGT+TGG | 0.585826 | 5.3:-72789339 | MS.gene47682:CDS |
| AGCAGAAGCTACAATTGAGA+GGG | 0.593359 | 5.3:-72789297 | MS.gene47682:CDS |
| TAGCAGAAGCTACAATTGAG+AGG | 0.609404 | 5.3:-72789298 | MS.gene47682:CDS |
| AATGCCATCATTGTGCCAGA+AGG | 0.618440 | 5.3:-72789260 | MS.gene47682:CDS |
| ACAATGATGGCATTCACCAA+TGG | 0.635361 | 5.3:+72789269 | None:intergenic |
| CCTGTCACAGCGGAAGTCGG+CGG | 0.661655 | 5.3:+72789226 | None:intergenic |
| AAAATGTCTGATGAATGCAA+AGG | 0.675933 | 5.3:-72789651 | MS.gene47682:intron |
| AAACCCTAACCCTGTCACAG+CGG | 0.735906 | 5.3:+72789216 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGAATTAATCAATATTGTA+TGG | - | chr5.3:72789450-72789469 | MS.gene47682:intron | 15.0% |
| !!! | CACAAACTATTTAATTTAAG+AGG | + | chr5.3:72789407-72789426 | None:intergenic | 20.0% |
| !!! | TCATTAAAGTTGTTTGATTT+TGG | - | chr5.3:72789365-72789384 | MS.gene47682:intron | 20.0% |
| ! | ATAACAATAGAGAGAGAAAA+AGG | + | chr5.3:72789315-72789334 | None:intergenic | 25.0% |
| ! | CAATAAGAAGAGAGAAAAAA+AGG | - | chr5.3:72789339-72789358 | MS.gene47682:CDS | 25.0% |
| ! | TCACAAAAGTTGTTTAAAAG+TGG | + | chr5.3:72789247-72789266 | None:intergenic | 25.0% |
| ! | TTAATCAATATTGTATGGTC+AGG | - | chr5.3:72789455-72789474 | MS.gene47682:intron | 25.0% |
| !!! | CTTTTTTTCTCTCTTCTTAT+TGG | + | chr5.3:72789341-72789360 | None:intergenic | 25.0% |
| TAAAGATGGAATTGTCTATC+AGG | - | chr5.3:72789631-72789650 | MS.gene47682:intron | 30.0% | |
| AATTGAGAGGGAGAATCCAT+TGG | - | chr5.3:72789532-72789551 | MS.gene47682:intron | 40.0% | |
| ACAATGATGGCATTCACCAA+TGG | + | chr5.3:72789551-72789570 | None:intergenic | 40.0% | |
| AGCAGAAGCTACAATTGAGA+GGG | - | chr5.3:72789520-72789539 | MS.gene47682:intron | 40.0% | |
| GTCTATCAGGTTCCAGTAAT+TGG | - | chr5.3:72789644-72789663 | MS.gene47682:intron | 40.0% | |
| GTCTGATGAATGCAAAGGTA+AGG | - | chr5.3:72789171-72789190 | MS.gene47682:CDS | 40.0% | |
| TAGCAGAAGCTACAATTGAG+AGG | - | chr5.3:72789519-72789538 | MS.gene47682:intron | 40.0% | |
| !! | AGGGTTTGGGTTGATAAAGA+TGG | - | chr5.3:72789617-72789636 | MS.gene47682:intron | 40.0% |
| AATGCCATCATTGTGCCAGA+AGG | - | chr5.3:72789557-72789576 | MS.gene47682:intron | 45.0% | |
| AGATCCTTCTGGCACAATGA+TGG | + | chr5.3:72789564-72789583 | None:intergenic | 45.0% | |
| TATGGTCAGGTAAGAGCTCA+TGG | - | chr5.3:72789468-72789487 | MS.gene47682:intron | 45.0% | |
| !!! | AAATTTTAATTATAATTTGA+TGG | - | chr5.3:72789427-72789446 | MS.gene47682:intron | 5.0% |
| AAACCCTAACCCTGTCACAG+CGG | + | chr5.3:72789604-72789623 | None:intergenic | 50.0% | |
| CTGTGACAGGGTTAGGGTTT+GGG | - | chr5.3:72789604-72789623 | MS.gene47682:intron | 50.0% | |
| GCGGTGACAAAAGATCCTTC+TGG | + | chr5.3:72789575-72789594 | None:intergenic | 50.0% | |
| GTTGGTTGGAGTGGAAGGAA+AGG | - | chr5.3:72789496-72789515 | MS.gene47682:intron | 50.0% | |
| TAAGAGCTCATGGCCAGAGT+TGG | - | chr5.3:72789478-72789497 | MS.gene47682:intron | 50.0% | |
| AACCCTGTCACAGCGGAAGT+CGG | + | chr5.3:72789597-72789616 | None:intergenic | 55.0% | |
| ACTTCCGCTGTGACAGGGTT+AGG | - | chr5.3:72789597-72789616 | MS.gene47682:intron | 55.0% | |
| CCTTCCACTCCAACCAACTC+TGG | + | chr5.3:72789494-72789513 | None:intergenic | 55.0% | |
| CTTCCGCTGTGACAGGGTTA+GGG | - | chr5.3:72789598-72789617 | MS.gene47682:intron | 55.0% | |
| GCTGTGACAGGGTTAGGGTT+TGG | - | chr5.3:72789603-72789622 | MS.gene47682:intron | 55.0% | |
| ! | AGCTCATGGCCAGAGTTGGT+TGG | - | chr5.3:72789482-72789501 | MS.gene47682:intron | 55.0% |
| ! | ATGGCCAGAGTTGGTTGGAG+TGG | - | chr5.3:72789487-72789506 | MS.gene47682:intron | 55.0% |
| ! | CCAGAGTTGGTTGGAGTGGA+AGG | - | chr5.3:72789491-72789510 | MS.gene47682:intron | 55.0% |
| CCTGTCACAGCGGAAGTCGG+CGG | + | chr5.3:72789594-72789613 | None:intergenic | 65.0% | |
| !! | CGCCGACTTCCGCTGTGACA+GGG | - | chr5.3:72789592-72789611 | MS.gene47682:intron | 65.0% |
| !! | CCGCCGACTTCCGCTGTGAC+AGG | - | chr5.3:72789591-72789610 | MS.gene47682:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 72789169 | 72789670 | 72789169 | ID=MS.gene47682 |
| chr5.3 | mRNA | 72789169 | 72789670 | 72789169 | ID=MS.gene47682.t1;Parent=MS.gene47682 |
| chr5.3 | exon | 72789652 | 72789670 | 72789652 | ID=MS.gene47682.t1.exon1;Parent=MS.gene47682.t1 |
| chr5.3 | CDS | 72789652 | 72789670 | 72789652 | ID=cds.MS.gene47682.t1;Parent=MS.gene47682.t1 |
| chr5.3 | exon | 72789169 | 72789362 | 72789169 | ID=MS.gene47682.t1.exon2;Parent=MS.gene47682.t1 |
| chr5.3 | CDS | 72789169 | 72789362 | 72789169 | ID=cds.MS.gene47682.t1;Parent=MS.gene47682.t1 |
| Gene Sequence |
| Protein sequence |
