AlfalfaGEDB | MS.gene52993

M. sativa cultivar XinJiangDaYe / MS.gene52993

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0006950 GO:0008150 GO:0009611 GO:0030234 GO:0030414 GO:0050896 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00280.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52993.t1	XP_003590795.1	90	70	7	0	1	70	1	70	1.80E-29	137.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52993.t1	P19873	62.1	66	25	0	5	70	3	68	1.0e-17	90.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52993.t1	G7I3Z2	90.0	70	7	0	1	70	1	70	1.3e-29	137.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055150	MS.gene52993	0.800418	1.46E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene004813	MS.gene52993	PPI
MS.gene066013	MS.gene52993	PPI
MS.gene017021	MS.gene52993	PPI
MS.gene017022	MS.gene52993	PPI
MS.gene52993	MS.gene47682	PPI
MS.gene036131	MS.gene52993	PPI
MS.gene038093	MS.gene52993	PPI
MS.gene47590	MS.gene52993	PPI
MS.gene39886	MS.gene52993	PPI
MS.gene038231	MS.gene52993	PPI
MS.gene71446	MS.gene52993	PPI
MS.gene49324	MS.gene52993	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52993.t1	MTR_1g075340	90.000	70	7	1	70	1	70	5.29e-42	131
MS.gene52993.t1	MTR_1g075340	90.000	70	7	1	70	1	70	5.29e-42	131
MS.gene52993.t1	MTR_1g075410	83.824	68	11	3	70	5	72	1.84e-36	117
MS.gene52993.t1	MTR_5g090250	84.286	70	11	1	70	1	70	2.74e-36	116
MS.gene52993.t1	MTR_5g090250	84.286	70	11	1	70	1	70	2.74e-36	116
MS.gene52993.t1	MTR_1g075380	79.412	68	14	3	70	4	71	8.44e-32	105
MS.gene52993.t1	MTR_1g070335	54.286	70	32	1	70	1	70	8.93e-22	80.1
MS.gene52993.t1	MTR_5g045470	53.731	67	31	4	70	3	69	6.76e-19	72.8
MS.gene52993.t1	MTR_1g070330	55.556	54	24	17	70	1	54	1.36e-15	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52993.t1	AT2G38870	52.857	70	33	0	1	70	1	70	1.45e-21	79.7
MS.gene52993.t1	AT5G43580	55.556	72	30	1	1	70	28	99	4.85e-21	79.0
MS.gene52993.t1	AT3G46860	46.032	63	34	0	8	70	23	85	3.97e-13	58.5
MS.gene52993.t1	AT5G43570	46.575	73	33	1	1	67	1	73	6.03e-12	56.2
MS.gene52993.t1	AT2G38900	42.188	64	37	0	7	70	10	73	2.04e-11	53.9
MS.gene52993.t1	AT2G38900	42.188	64	37	0	7	70	25	88	3.10e-11	53.9
MS.gene52993.t1	AT5G43570	50.000	60	30	0	8	67	24	83	6.40e-11	53.9

Find 26 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTCCACTCCAACCAATTC+TGG	0.174728	1.1:-56411840	None:intergenic
CTGTGACAGGGTTTGGGTTT+GGG	0.259063	1.1:+56411953	MS.gene52993:CDS
ACTTCCGCTGTGACAGGGTT+TGG	0.267379	1.1:+56411946	MS.gene52993:CDS
CTTCCGCTGTGACAGGGTTT+GGG	0.309008	1.1:+56411947	MS.gene52993:CDS
GCTGTGACAGGGTTTGGGTT+TGG	0.326505	1.1:+56411952	MS.gene52993:CDS
TAAAGATGGAATTGTCTATC+AGG	0.326953	1.1:+56411980	MS.gene52993:CDS
CCTTTGACTTCCGCTGTGAC+AGG	0.333094	1.1:+56411940	MS.gene52993:CDS
TAAGAGTTCATGGCCAGAAT+TGG	0.414180	1.1:+56411827	MS.gene52993:CDS
TGGGTTTGGGTTAATAAAGA+TGG	0.418377	1.1:+56411966	MS.gene52993:CDS
TGTGGTCAGGTAAGAGTTCA+TGG	0.426967	1.1:+56411817	MS.gene52993:intron
CTTTGACTTCCGCTGTGACA+GGG	0.463269	1.1:+56411941	MS.gene52993:CDS
CTGTCACAGCGGAAGTCAAA+GGG	0.467050	1.1:-56411939	None:intergenic
GATGCCATCATTGTGCTAGA+AGG	0.504821	1.1:+56411906	MS.gene52993:CDS
CCAGAATTGGTTGGAGTGGA+AGG	0.515293	1.1:+56411840	MS.gene52993:CDS
AGTTCCTTCTAGCACAATGA+TGG	0.538007	1.1:-56411910	None:intergenic
AATTCAGAGGGAGAATCCAT+TGG	0.542280	1.1:+56411881	MS.gene52993:CDS
CCTGTCACAGCGGAAGTCAA+AGG	0.545493	1.1:-56411940	None:intergenic
ATGGCCAGAATTGGTTGGAG+TGG	0.561764	1.1:+56411836	MS.gene52993:CDS
AGTTCATGGCCAGAATTGGT+TGG	0.565712	1.1:+56411831	MS.gene52993:CDS
ATTGGTTGGAGTGGAAGGAA+AGG	0.570071	1.1:+56411845	MS.gene52993:CDS
AGCAGAAGCTACAATTCAGA+GGG	0.593359	1.1:+56411869	MS.gene52993:CDS
TAGCAGAAGCTACAATTCAG+AGG	0.596029	1.1:+56411868	MS.gene52993:CDS
ACAATGATGGCATCAACCAA+TGG	0.650325	1.1:-56411897	None:intergenic
AAAATGTCTGATGAATGCAA+AGG	0.675933	1.1:+56411497	None:intergenic
TGTCACAGCGGAAGTCAAAG+GGG	0.698463	1.1:-56411938	None:intergenic
AAACCCAAACCCTGTCACAG+CGG	0.730650	1.1:-56411950	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATATGAATAATAAATTGAA+TGG	+	chr1.1:56411747-56411766	MS.gene52993:intron	10.0%
!!	ATGTAACCAAAAAATATATA+TGG	-	chr1.1:56411719-56411738	None:intergenic	15.0%
!!	TGTAACCAAAAAATATATAT+GGG	-	chr1.1:56411718-56411737	None:intergenic	15.0%
!!!	CAACTTTTCAAAAATATAAA+AGG	-	chr1.1:56411644-56411663	None:intergenic	15.0%
!!!	CATTACCCATATATATTTTT+TGG	+	chr1.1:56411710-56411729	MS.gene52993:intron	20.0%
!	ATGCTTACATGCATATATAT+TGG	+	chr1.1:56411545-56411564	MS.gene52993:intron	25.0%
!	TGCTTACATGCATATATATT+GGG	+	chr1.1:56411546-56411565	MS.gene52993:intron	25.0%
!	TTGAATGAATCAATATTGTG+TGG	+	chr1.1:56411799-56411818	MS.gene52993:intron	25.0%
	TAAAGATGGAATTGTCTATC+AGG	+	chr1.1:56411980-56411999	MS.gene52993:CDS	30.0%
	ATGAATGCAAAGGTAAGGTA+AGG	+	chr1.1:56411507-56411526	MS.gene52993:intron	35.0%
	GAATGTAATACTTACCACAC+TGG	+	chr1.1:56411683-56411702	MS.gene52993:intron	35.0%
	GTCTATCAGGTTCCAAAAAT+TGG	+	chr1.1:56411993-56412012	MS.gene52993:CDS	35.0%
	TGAATCAATATTGTGTGGTC+AGG	+	chr1.1:56411804-56411823	MS.gene52993:intron	35.0%
!	TGGGTTTGGGTTAATAAAGA+TGG	+	chr1.1:56411966-56411985	MS.gene52993:CDS	35.0%
	AATTCAGAGGGAGAATCCAT+TGG	+	chr1.1:56411881-56411900	MS.gene52993:CDS	40.0%
	ACAATGATGGCATCAACCAA+TGG	-	chr1.1:56411900-56411919	None:intergenic	40.0%
	AGCAGAAGCTACAATTCAGA+GGG	+	chr1.1:56411869-56411888	MS.gene52993:CDS	40.0%
	AGTTCCTTCTAGCACAATGA+TGG	-	chr1.1:56411913-56411932	None:intergenic	40.0%
	ATGGGTAATGTTAACCAGTG+TGG	-	chr1.1:56411700-56411719	None:intergenic	40.0%
	GTCTGATGAATGCAAAGGTA+AGG	+	chr1.1:56411502-56411521	MS.gene52993:intron	40.0%
	TAAGAGTTCATGGCCAGAAT+TGG	+	chr1.1:56411827-56411846	MS.gene52993:CDS	40.0%
	TAGCAGAAGCTACAATTCAG+AGG	+	chr1.1:56411868-56411887	MS.gene52993:CDS	40.0%
	AGTTCATGGCCAGAATTGGT+TGG	+	chr1.1:56411831-56411850	MS.gene52993:CDS	45.0%
	ATTGGTTGGAGTGGAAGGAA+AGG	+	chr1.1:56411845-56411864	MS.gene52993:CDS	45.0%
	GATGCCATCATTGTGCTAGA+AGG	+	chr1.1:56411906-56411925	MS.gene52993:CDS	45.0%
	TGTGGTCAGGTAAGAGTTCA+TGG	+	chr1.1:56411817-56411836	MS.gene52993:intron	45.0%
	AAACCCAAACCCTGTCACAG+CGG	-	chr1.1:56411953-56411972	None:intergenic	50.0%
	AGTTTCCTTAACCAGTGCCC+CGG	-	chr1.1:56411590-56411609	None:intergenic	50.0%
	ATGGCCAGAATTGGTTGGAG+TGG	+	chr1.1:56411836-56411855	MS.gene52993:CDS	50.0%
	CCAGAATTGGTTGGAGTGGA+AGG	+	chr1.1:56411840-56411859	MS.gene52993:CDS	50.0%
	CCTTCCACTCCAACCAATTC+TGG	-	chr1.1:56411843-56411862	None:intergenic	50.0%
	CTGTCACAGCGGAAGTCAAA+GGG	-	chr1.1:56411942-56411961	None:intergenic	50.0%
	TGTCACAGCGGAAGTCAAAG+GGG	-	chr1.1:56411941-56411960	None:intergenic	50.0%
!	CTGTGACAGGGTTTGGGTTT+GGG	+	chr1.1:56411953-56411972	MS.gene52993:CDS	50.0%
!	CTTTGACTTCCGCTGTGACA+GGG	+	chr1.1:56411941-56411960	MS.gene52993:CDS	50.0%
	ACTTCCGCTGTGACAGGGTT+TGG	+	chr1.1:56411946-56411965	MS.gene52993:CDS	55.0%
	CCTGTCACAGCGGAAGTCAA+AGG	-	chr1.1:56411943-56411962	None:intergenic	55.0%
	CTTCCGCTGTGACAGGGTTT+GGG	+	chr1.1:56411947-56411966	MS.gene52993:CDS	55.0%
	GTCATGTTAACCAGTGCCCC+CGG	+	chr1.1:56411568-56411587	MS.gene52993:intron	55.0%
	GTTTCCTTAACCAGTGCCCC+GGG	-	chr1.1:56411589-56411608	None:intergenic	55.0%
	TCATGTTAACCAGTGCCCCC+GGG	+	chr1.1:56411569-56411588	MS.gene52993:intron	55.0%
	TTTCCTTAACCAGTGCCCCG+GGG	-	chr1.1:56411588-56411607	None:intergenic	55.0%
!	CCTTTGACTTCCGCTGTGAC+AGG	+	chr1.1:56411940-56411959	MS.gene52993:CDS	55.0%
!	GCTGTGACAGGGTTTGGGTT+TGG	+	chr1.1:56411952-56411971	MS.gene52993:CDS	55.0%
	CATGTTAACCAGTGCCCCCG+GGG	+	chr1.1:56411570-56411589	MS.gene52993:intron	60.0%
	TTCCTTAACCAGTGCCCCGG+GGG	-	chr1.1:56411587-56411606	None:intergenic	60.0%
!!	TGCCCCCGGGGCACTGGTTA+AGG	+	chr1.1:56411582-56411601	MS.gene52993:intron	70.0%
	AACCAGTGCCCCCGGGGCAC+TGG	+	chr1.1:56411576-56411595	MS.gene52993:intron	75.0%
!	AACCAGTGCCCCGGGGGCAC+TGG	-	chr1.1:56411581-56411600	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	56411500	56412019	56411500	ID=MS.gene52993
chr1.1	mRNA	56411500	56412019	56411500	ID=MS.gene52993.t1;Parent=MS.gene52993
chr1.1	exon	56411500	56411518	56411500	ID=MS.gene52993.t1.exon1;Parent=MS.gene52993.t1
chr1.1	CDS	56411500	56411518	56411500	ID=cds.MS.gene52993.t1;Parent=MS.gene52993.t1
chr1.1	exon	56411826	56412019	56411826	ID=MS.gene52993.t1.exon2;Parent=MS.gene52993.t1
chr1.1	CDS	56411826	56412019	56411826	ID=cds.MS.gene52993.t1;Parent=MS.gene52993.t1

Gene Sequence

>MS.gene52993

ATGTCTGATGAATGCAAAGGTAAGGTAAGGTGTTATAATATAAATATGCTTACATGCATATATATTGGGTCATGTTAACCAGTGCCCCCGGGGCACTGGTTAAGGAAACTAAAAAAAGAAAGTTATAAAATGAAAGTAACACCTTTTATATTTTTGAAAAGTTGACAGCACAAGTTATAAAATGAATGTAATACTTACCACACTGGTTAACATTACCCATATATATTTTTTGGTTACATTACATTCATATATGAATAATAAATTGAATGGTTTGTGAAATTTTTATTATAACTTGATCGTTGAATGAATCAATATTGTGTGGTCAGGTAAGAGTTCATGGCCAGAATTGGTTGGAGTGGAAGGAAAGGTAGCAGAAGCTACAATTCAGAGGGAGAATCCATTGGTTGATGCCATCATTGTGCTAGAAGGAACTGATGTCCCCTTTGACTTCCGCTGTGACAGGGTTTGGGTTTGGGTTAATAAAGATGGAATTGTCTATCAGGTTCCAAAAATTGGATAG

Protein sequence

>MS.gene52993.t1

MSDECKGKSSWPELVGVEGKVAEATIQRENPLVDAIIVLEGTDVPFDFRCDRVWVWVNKDGIVYQVPKIG