AlfalfaGEDB | MS.gene71446

M. sativa cultivar XinJiangDaYe / MS.gene71446

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0006950 GO:0008150 GO:0009611 GO:0030234 GO:0030414 GO:0050896 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00280.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71446.t1	XP_003590795.1	90	70	7	0	1	70	1	70	1.80E-29	137.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71446.t1	P19873	62.1	66	25	0	5	70	3	68	1.0e-17	90.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71446.t1	G7I3Z2	90.0	70	7	0	1	70	1	70	1.3e-29	137.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene036131	MS.gene71446	PPI
MS.gene39886	MS.gene71446	PPI
MS.gene71446	MS.gene47682	PPI
MS.gene038231	MS.gene71446	PPI
MS.gene038093	MS.gene71446	PPI
MS.gene71446	MS.gene038093	PPI
MS.gene49324	MS.gene71446	PPI
MS.gene004813	MS.gene71446	PPI
MS.gene71446	MS.gene47590	PPI
MS.gene71446	MS.gene017021	PPI
MS.gene066013	MS.gene71446	PPI
MS.gene017021	MS.gene71446	PPI
MS.gene017022	MS.gene71446	PPI
MS.gene71446	MS.gene52993	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71446.t1	MTR_1g075340	90.000	70	7	1	70	1	70	5.29e-42	131
MS.gene71446.t1	MTR_1g075340	90.000	70	7	1	70	1	70	5.29e-42	131
MS.gene71446.t1	MTR_1g075410	83.824	68	11	3	70	5	72	1.84e-36	117
MS.gene71446.t1	MTR_5g090250	84.286	70	11	1	70	1	70	2.74e-36	116
MS.gene71446.t1	MTR_5g090250	84.286	70	11	1	70	1	70	2.74e-36	116
MS.gene71446.t1	MTR_1g075380	79.412	68	14	3	70	4	71	8.44e-32	105
MS.gene71446.t1	MTR_1g070335	54.286	70	32	1	70	1	70	8.93e-22	80.1
MS.gene71446.t1	MTR_5g045470	53.731	67	31	4	70	3	69	6.76e-19	72.8
MS.gene71446.t1	MTR_1g070330	55.556	54	24	17	70	1	54	1.36e-15	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71446.t1	AT2G38870	52.857	70	33	0	1	70	1	70	1.45e-21	79.7
MS.gene71446.t1	AT5G43580	55.556	72	30	1	1	70	28	99	4.85e-21	79.0
MS.gene71446.t1	AT3G46860	46.032	63	34	0	8	70	23	85	3.97e-13	58.5
MS.gene71446.t1	AT5G43570	46.575	73	33	1	1	67	1	73	6.03e-12	56.2
MS.gene71446.t1	AT2G38900	42.188	64	37	0	7	70	10	73	2.04e-11	53.9
MS.gene71446.t1	AT2G38900	42.188	64	37	0	7	70	25	88	3.10e-11	53.9
MS.gene71446.t1	AT5G43570	50.000	60	30	0	8	67	24	83	6.40e-11	53.9

Find 26 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGTGACAGGGTTTGGGTTT+GGG	0.259063	1.3:+53920216	MS.gene71446:CDS
ACTTCCGCTGTGACAGGGTT+TGG	0.267379	1.3:+53920209	MS.gene71446:CDS
CTTCCGCTGTGACAGGGTTT+GGG	0.309008	1.3:+53920210	MS.gene71446:CDS
GCTGTGACAGGGTTTGGGTT+TGG	0.326505	1.3:+53920215	MS.gene71446:CDS
TAAAGATGGAATTGTCTATC+AGG	0.326953	1.3:+53920243	MS.gene71446:CDS
CCTTTGACTTCCGCTGTGAC+AGG	0.333094	1.3:+53920203	MS.gene71446:CDS
TGGGTTTGGGTTAATAAAGA+TGG	0.418377	1.3:+53920229	MS.gene71446:CDS
CCTTCCACTCCAACCAACTC+TGG	0.454716	1.3:-53920103	None:intergenic
CTTTGACTTCCGCTGTGACA+GGG	0.463269	1.3:+53920204	MS.gene71446:CDS
CTGTCACAGCGGAAGTCAAA+GGG	0.467050	1.3:-53920202	None:intergenic
TGTGCTCAGGTAAGAGTTCA+TGG	0.479878	1.3:+53920080	MS.gene71446:intron
CCAGAGTTGGTTGGAGTGGA+AGG	0.515293	1.3:+53920103	MS.gene71446:CDS
GATGCCATCATTGTACTAGA+AGG	0.519670	1.3:+53920169	MS.gene71446:CDS
GTTGGTTGGAGTGGAAGGAA+AGG	0.527079	1.3:+53920108	MS.gene71446:CDS
AATTCAGAGGGAGAATCCAT+TGG	0.542280	1.3:+53920144	MS.gene71446:CDS
ATGGCCAGAGTTGGTTGGAG+TGG	0.545068	1.3:+53920099	MS.gene71446:CDS
CCTGTCACAGCGGAAGTCAA+AGG	0.545493	1.3:-53920203	None:intergenic
AGTTCCTTCTAGTACAATGA+TGG	0.549173	1.3:-53920173	None:intergenic
TAAGAGTTCATGGCCAGAGT+TGG	0.563662	1.3:+53920090	MS.gene71446:CDS
AGTTCATGGCCAGAGTTGGT+TGG	0.565712	1.3:+53920094	MS.gene71446:CDS
AGCAGAAGCTACAATTCAGA+GGG	0.593359	1.3:+53920132	MS.gene71446:CDS
TAGCAGAAGCTACAATTCAG+AGG	0.596029	1.3:+53920131	MS.gene71446:CDS
ACAATGATGGCATCAACCAA+TGG	0.612007	1.3:-53920160	None:intergenic
AAAATGTCTGATGAATGCAA+AGG	0.675933	1.3:+53919959	None:intergenic
TGTCACAGCGGAAGTCAAAG+GGG	0.698463	1.3:-53920201	None:intergenic
AAACCCAAACCCTGTCACAG+CGG	0.730650	1.3:-53920213	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAAAGATGGAATTGTCTATC+AGG	+	chr1.3:53920243-53920262	MS.gene71446:CDS	30.0%
	AGTTCCTTCTAGTACAATGA+TGG	-	chr1.3:53920176-53920195	None:intergenic	35.0%
	GTCTATCAGGTTCCAAAAAT+TGG	+	chr1.3:53920256-53920275	MS.gene71446:CDS	35.0%
	TCAATATATTGTGTGTGCTC+AGG	+	chr1.3:53920067-53920086	MS.gene71446:intron	35.0%
!	TGGGTTTGGGTTAATAAAGA+TGG	+	chr1.3:53920229-53920248	MS.gene71446:CDS	35.0%
	AATTCAGAGGGAGAATCCAT+TGG	+	chr1.3:53920144-53920163	MS.gene71446:CDS	40.0%
	ACAATGATGGCATCAACCAA+TGG	-	chr1.3:53920163-53920182	None:intergenic	40.0%
	AGCAGAAGCTACAATTCAGA+GGG	+	chr1.3:53920132-53920151	MS.gene71446:CDS	40.0%
	GATGCCATCATTGTACTAGA+AGG	+	chr1.3:53920169-53920188	MS.gene71446:CDS	40.0%
	GTCTGATGAATGCAAAGGTA+AGG	+	chr1.3:53919964-53919983	MS.gene71446:intron	40.0%
	TAGCAGAAGCTACAATTCAG+AGG	+	chr1.3:53920131-53920150	MS.gene71446:CDS	40.0%
	TAAGAGTTCATGGCCAGAGT+TGG	+	chr1.3:53920090-53920109	MS.gene71446:CDS	45.0%
	TGTGCTCAGGTAAGAGTTCA+TGG	+	chr1.3:53920080-53920099	MS.gene71446:intron	45.0%
!!!	AAATTTTAATTATAATTTGA+TGG	+	chr1.3:53920035-53920054	MS.gene71446:intron	5.0%
	AAACCCAAACCCTGTCACAG+CGG	-	chr1.3:53920216-53920235	None:intergenic	50.0%
	CTGTCACAGCGGAAGTCAAA+GGG	-	chr1.3:53920205-53920224	None:intergenic	50.0%
	GTTGGTTGGAGTGGAAGGAA+AGG	+	chr1.3:53920108-53920127	MS.gene71446:CDS	50.0%
	TGTCACAGCGGAAGTCAAAG+GGG	-	chr1.3:53920204-53920223	None:intergenic	50.0%
!	AGTTCATGGCCAGAGTTGGT+TGG	+	chr1.3:53920094-53920113	MS.gene71446:CDS	50.0%
!	CTGTGACAGGGTTTGGGTTT+GGG	+	chr1.3:53920216-53920235	MS.gene71446:CDS	50.0%
!	CTTTGACTTCCGCTGTGACA+GGG	+	chr1.3:53920204-53920223	MS.gene71446:CDS	50.0%
	ACTTCCGCTGTGACAGGGTT+TGG	+	chr1.3:53920209-53920228	MS.gene71446:CDS	55.0%
	CCTGTCACAGCGGAAGTCAA+AGG	-	chr1.3:53920206-53920225	None:intergenic	55.0%
	CCTTCCACTCCAACCAACTC+TGG	-	chr1.3:53920106-53920125	None:intergenic	55.0%
	CTTCCGCTGTGACAGGGTTT+GGG	+	chr1.3:53920210-53920229	MS.gene71446:CDS	55.0%
!	ATGGCCAGAGTTGGTTGGAG+TGG	+	chr1.3:53920099-53920118	MS.gene71446:CDS	55.0%
!	CCAGAGTTGGTTGGAGTGGA+AGG	+	chr1.3:53920103-53920122	MS.gene71446:CDS	55.0%
!	CCTTTGACTTCCGCTGTGAC+AGG	+	chr1.3:53920203-53920222	MS.gene71446:CDS	55.0%
!	GCTGTGACAGGGTTTGGGTT+TGG	+	chr1.3:53920215-53920234	MS.gene71446:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	53919962	53920282	53919962	ID=MS.gene71446
chr1.3	mRNA	53919962	53920282	53919962	ID=MS.gene71446.t1;Parent=MS.gene71446
chr1.3	exon	53919962	53919980	53919962	ID=MS.gene71446.t1.exon1;Parent=MS.gene71446.t1
chr1.3	CDS	53919962	53919980	53919962	ID=cds.MS.gene71446.t1;Parent=MS.gene71446.t1
chr1.3	exon	53920089	53920282	53920089	ID=MS.gene71446.t1.exon2;Parent=MS.gene71446.t1
chr1.3	CDS	53920089	53920282	53920089	ID=cds.MS.gene71446.t1;Parent=MS.gene71446.t1

Gene Sequence	>MS.gene71446 ATGTCTGATGAATGCAAAGGTAAGGTGTTATAATATAATATGTTTACATGCATACATATGAATAATAAATTAAAAATTTTAATTATAATTTGATGGTTGAATGAATCAATATATTGTGTGTGCTCAGGTAAGAGTTCATGGCCAGAGTTGGTTGGAGTGGAAGGAAAGGTAGCAGAAGCTACAATTCAGAGGGAGAATCCATTGGTTGATGCCATCATTGTACTAGAAGGAACTGATGTCCCCTTTGACTTCCGCTGTGACAGGGTTTGGGTTTGGGTTAATAAAGATGGAATTGTCTATCAGGTTCCAAAAATTGGATAG
Protein sequence	>MS.gene71446.t1 MSDECKGKSSWPELVGVEGKVAEATIQRENPLVDAIIVLEGTDVPFDFRCDRVWVWVNKDGIVYQVPKIG