Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038231.t1 | AET00273.1 | 92.9 | 70 | 5 | 0 | 1 | 70 | 1 | 70 | 8.20E-30 | 139 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038231.t1 | P24076 | 65.7 | 67 | 23 | 0 | 4 | 70 | 2 | 68 | 1.6e-18 | 92.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038231.t1 | G7KCZ8 | 92.9 | 70 | 5 | 0 | 1 | 70 | 1 | 70 | 5.9e-30 | 139.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038231 | MS.gene47682 | PPI |
| MS.gene038231 | MS.gene036131 | PPI |
| MS.gene038231 | MS.gene71446 | PPI |
| MS.gene038231 | MS.gene017022 | PPI |
| MS.gene038231 | MS.gene038093 | PPI |
| MS.gene004813 | MS.gene038231 | PPI |
| MS.gene038231 | MS.gene47590 | PPI |
| MS.gene038231 | MS.gene49324 | PPI |
| MS.gene038231 | MS.gene066013 | PPI |
| MS.gene038231 | MS.gene017021 | PPI |
| MS.gene038231 | MS.gene52993 | PPI |
| MS.gene39886 | MS.gene038231 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038231.t1 | MTR_5g090250 | 92.857 | 70 | 5 | 0 | 1 | 70 | 1 | 70 | 1.94e-43 | 134 |
| MS.gene038231.t1 | MTR_5g090250 | 92.857 | 70 | 5 | 0 | 1 | 70 | 1 | 70 | 1.94e-43 | 134 |
| MS.gene038231.t1 | MTR_1g075410 | 89.706 | 68 | 7 | 0 | 3 | 70 | 5 | 72 | 5.59e-39 | 123 |
| MS.gene038231.t1 | MTR_1g075340 | 84.286 | 70 | 11 | 0 | 1 | 70 | 1 | 70 | 6.06e-38 | 120 |
| MS.gene038231.t1 | MTR_1g075340 | 84.286 | 70 | 11 | 0 | 1 | 70 | 1 | 70 | 6.06e-38 | 120 |
| MS.gene038231.t1 | MTR_1g075380 | 82.353 | 68 | 12 | 0 | 3 | 70 | 4 | 71 | 1.75e-33 | 109 |
| MS.gene038231.t1 | MTR_5g045470 | 63.636 | 66 | 24 | 0 | 5 | 70 | 4 | 69 | 1.71e-24 | 87.0 |
| MS.gene038231.t1 | MTR_1g070335 | 61.429 | 70 | 27 | 0 | 1 | 70 | 1 | 70 | 2.14e-24 | 86.7 |
| MS.gene038231.t1 | MTR_1g070330 | 59.259 | 54 | 22 | 0 | 17 | 70 | 1 | 54 | 1.16e-15 | 64.3 |
| MS.gene038231.t1 | MTR_3g020970 | 49.206 | 63 | 32 | 0 | 8 | 70 | 36 | 98 | 2.81e-13 | 59.3 |
| MS.gene038231.t1 | MTR_3g020800 | 47.619 | 63 | 33 | 0 | 8 | 70 | 36 | 98 | 2.48e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038231.t1 | AT2G38870 | 60.000 | 70 | 28 | 0 | 1 | 70 | 1 | 70 | 4.01e-24 | 85.9 |
| MS.gene038231.t1 | AT5G43580 | 56.250 | 64 | 28 | 0 | 7 | 70 | 36 | 99 | 1.62e-19 | 75.1 |
| MS.gene038231.t1 | AT2G38900 | 48.438 | 64 | 33 | 0 | 7 | 70 | 25 | 88 | 7.92e-16 | 65.5 |
| MS.gene038231.t1 | AT2G38900 | 48.438 | 64 | 33 | 0 | 7 | 70 | 10 | 73 | 8.23e-16 | 65.1 |
| MS.gene038231.t1 | AT3G46860 | 47.619 | 63 | 33 | 0 | 8 | 70 | 23 | 85 | 9.93e-14 | 60.1 |
| MS.gene038231.t1 | AT5G43570 | 47.297 | 74 | 33 | 1 | 1 | 68 | 1 | 74 | 1.60e-13 | 60.1 |
| MS.gene038231.t1 | AT5G43570 | 50.820 | 61 | 30 | 0 | 8 | 68 | 24 | 84 | 1.67e-12 | 57.8 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTATCAGGTTCCAGTAAT+TGG | 0.190800 | 5.4:-71325288 | MS.gene038231:CDS |
| CTGTGACAGGGTTAGGGTTT+GGG | 0.262714 | 5.4:-71325328 | MS.gene038231:CDS |
| GACACTTTCTATCCAATTAC+TGG | 0.267846 | 5.4:+71325276 | None:intergenic |
| ACTTCCGCTGTGACAGGGTT+AGG | 0.276358 | 5.4:-71325335 | MS.gene038231:CDS |
| TAAAGATGGAATTGTCTATC+AGG | 0.346883 | 5.4:-71325301 | MS.gene038231:CDS |
| GCTGTGACAGGGTTAGGGTT+TGG | 0.358066 | 5.4:-71325329 | MS.gene038231:CDS |
| AGGGTTTGGGTTGATAAAGA+TGG | 0.412719 | 5.4:-71325315 | MS.gene038231:CDS |
| CCGCCGACTTCCGCTGTGAC+AGG | 0.421191 | 5.4:-71325341 | MS.gene038231:CDS |
| CTTCCGCTGTGACAGGGTTA+GGG | 0.422816 | 5.4:-71325334 | MS.gene038231:CDS |
| CCTTCCACTCCAACCAACTC+TGG | 0.454716 | 5.4:+71325441 | None:intergenic |
| CCAGAGTTGGTTGGAGTGGA+AGG | 0.515293 | 5.4:-71325441 | MS.gene038231:CDS |
| GTTGGTTGGAGTGGAAGGAA+AGG | 0.527079 | 5.4:-71325436 | MS.gene038231:CDS |
| AGATCCTTCTGGCACAATGA+TGG | 0.529456 | 5.4:+71325371 | None:intergenic |
| GCGGTGACAACAGATCCTTC+TGG | 0.532461 | 5.4:+71325360 | None:intergenic |
| TATGGTCAGGTAAGAGCTCA+TGG | 0.552544 | 5.4:-71325464 | MS.gene038231:intron |
| CGCCGACTTCCGCTGTGACA+GGG | 0.553611 | 5.4:-71325340 | MS.gene038231:CDS |
| ATGGCCAGAGTTGGTTGGAG+TGG | 0.564909 | 5.4:-71325445 | MS.gene038231:CDS |
| AATTGAGAGGGAGAATCCAT+TGG | 0.570404 | 5.4:-71325400 | MS.gene038231:CDS |
| AACCCTGTCACAGCGGAAGT+CGG | 0.575336 | 5.4:+71325338 | None:intergenic |
| AGCTCATGGCCAGAGTTGGT+TGG | 0.578782 | 5.4:-71325450 | MS.gene038231:CDS |
| TAAGAGCTCATGGCCAGAGT+TGG | 0.585826 | 5.4:-71325454 | MS.gene038231:CDS |
| AGCAGAAGCTACAATTGAGA+GGG | 0.593359 | 5.4:-71325412 | MS.gene038231:CDS |
| TAGCAGAAGCTACAATTGAG+AGG | 0.609404 | 5.4:-71325413 | MS.gene038231:CDS |
| AATGCCATCATTGTGCCAGA+AGG | 0.618440 | 5.4:-71325375 | MS.gene038231:CDS |
| ACAATGATGGCATTCACCAA+TGG | 0.635361 | 5.4:+71325384 | None:intergenic |
| CCTGTCACAGCGGAAGTCGG+CGG | 0.661655 | 5.4:+71325341 | None:intergenic |
| AAAATGTCTGATGAATGCAA+AGG | 0.675933 | 5.4:-71325573 | MS.gene038231:intron |
| AAACCCTAACCCTGTCACAG+CGG | 0.735906 | 5.4:+71325331 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATTTTAATTATAATTTGA+TGG | - | chr5.4:71325349-71325368 | MS.gene038231:CDS | 10.0% |
| !! | TTGAATTAATCAATATTGTA+TGG | - | chr5.4:71325372-71325391 | MS.gene038231:CDS | 15.0% |
| !! | TGTTTAAATTAAATGGTTTG+TGG | - | chr5.4:71325327-71325346 | MS.gene038231:CDS | 20.0% |
| ! | TTAATCAATATTGTATGGTC+AGG | - | chr5.4:71325377-71325396 | MS.gene038231:CDS | 25.0% |
| TAAAGATGGAATTGTCTATC+AGG | - | chr5.4:71325553-71325572 | MS.gene038231:intron | 30.0% | |
| AATTGAGAGGGAGAATCCAT+TGG | - | chr5.4:71325454-71325473 | MS.gene038231:CDS | 40.0% | |
| ACAATGATGGCATTCACCAA+TGG | + | chr5.4:71325473-71325492 | None:intergenic | 40.0% | |
| AGCAGAAGCTACAATTGAGA+GGG | - | chr5.4:71325442-71325461 | MS.gene038231:CDS | 40.0% | |
| GTCTATCAGGTTCCAGTAAT+TGG | - | chr5.4:71325566-71325585 | MS.gene038231:intron | 40.0% | |
| GTCTGATGAATGCAAAGGTA+AGG | - | chr5.4:71325286-71325305 | MS.gene038231:CDS | 40.0% | |
| TAGCAGAAGCTACAATTGAG+AGG | - | chr5.4:71325441-71325460 | MS.gene038231:CDS | 40.0% | |
| !! | AGGGTTTGGGTTGATAAAGA+TGG | - | chr5.4:71325539-71325558 | MS.gene038231:intron | 40.0% |
| AATGCCATCATTGTGCCAGA+AGG | - | chr5.4:71325479-71325498 | MS.gene038231:intron | 45.0% | |
| AGATCCTTCTGGCACAATGA+TGG | + | chr5.4:71325486-71325505 | None:intergenic | 45.0% | |
| TATGGTCAGGTAAGAGCTCA+TGG | - | chr5.4:71325390-71325409 | MS.gene038231:CDS | 45.0% | |
| !! | TATAATATGTTTAAATTAAA+TGG | - | chr5.4:71325320-71325339 | MS.gene038231:CDS | 5.0% |
| AAACCCTAACCCTGTCACAG+CGG | + | chr5.4:71325526-71325545 | None:intergenic | 50.0% | |
| CTGTGACAGGGTTAGGGTTT+GGG | - | chr5.4:71325526-71325545 | MS.gene038231:intron | 50.0% | |
| GTTGGTTGGAGTGGAAGGAA+AGG | - | chr5.4:71325418-71325437 | MS.gene038231:CDS | 50.0% | |
| TAAGAGCTCATGGCCAGAGT+TGG | - | chr5.4:71325400-71325419 | MS.gene038231:CDS | 50.0% | |
| AACCCTGTCACAGCGGAAGT+CGG | + | chr5.4:71325519-71325538 | None:intergenic | 55.0% | |
| ACTTCCGCTGTGACAGGGTT+AGG | - | chr5.4:71325519-71325538 | MS.gene038231:intron | 55.0% | |
| CCTTCCACTCCAACCAACTC+TGG | + | chr5.4:71325416-71325435 | None:intergenic | 55.0% | |
| CTTCCGCTGTGACAGGGTTA+GGG | - | chr5.4:71325520-71325539 | MS.gene038231:intron | 55.0% | |
| GCGGTGACAACAGATCCTTC+TGG | + | chr5.4:71325497-71325516 | None:intergenic | 55.0% | |
| GCTGTGACAGGGTTAGGGTT+TGG | - | chr5.4:71325525-71325544 | MS.gene038231:intron | 55.0% | |
| ! | AGCTCATGGCCAGAGTTGGT+TGG | - | chr5.4:71325404-71325423 | MS.gene038231:CDS | 55.0% |
| ! | ATGGCCAGAGTTGGTTGGAG+TGG | - | chr5.4:71325409-71325428 | MS.gene038231:CDS | 55.0% |
| ! | CCAGAGTTGGTTGGAGTGGA+AGG | - | chr5.4:71325413-71325432 | MS.gene038231:CDS | 55.0% |
| CCTGTCACAGCGGAAGTCGG+CGG | + | chr5.4:71325516-71325535 | None:intergenic | 65.0% | |
| !! | CGCCGACTTCCGCTGTGACA+GGG | - | chr5.4:71325514-71325533 | MS.gene038231:intron | 65.0% |
| !! | CCGCCGACTTCCGCTGTGAC+AGG | - | chr5.4:71325513-71325532 | MS.gene038231:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 71325284 | 71325592 | 71325284 | ID=MS.gene038231 |
| chr5.4 | mRNA | 71325284 | 71325592 | 71325284 | ID=MS.gene038231.t1;Parent=MS.gene038231 |
| chr5.4 | exon | 71325574 | 71325592 | 71325574 | ID=MS.gene038231.t1.exon1;Parent=MS.gene038231.t1 |
| chr5.4 | CDS | 71325574 | 71325592 | 71325574 | ID=cds.MS.gene038231.t1;Parent=MS.gene038231.t1 |
| chr5.4 | exon | 71325284 | 71325477 | 71325284 | ID=MS.gene038231.t1.exon2;Parent=MS.gene038231.t1 |
| chr5.4 | CDS | 71325284 | 71325477 | 71325284 | ID=cds.MS.gene038231.t1;Parent=MS.gene038231.t1 |
| Gene Sequence |
| Protein sequence |
