Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47590.t1 | AET00273.1 | 94.2 | 52 | 3 | 0 | 1 | 52 | 1 | 52 | 4.40E-20 | 106.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47590.t1 | P24076 | 67.3 | 49 | 16 | 0 | 4 | 52 | 2 | 50 | 2.2e-12 | 72.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47590.t1 | G7KCZ8 | 94.2 | 52 | 3 | 0 | 1 | 52 | 1 | 52 | 3.1e-20 | 106.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058493 | MS.gene47590 | 0.802713 | 4.92E-49 | -1.69E-46 |
| MS.gene05884 | MS.gene47590 | 0.819145 | 1.35E-52 | -1.69E-46 |
| MS.gene059514 | MS.gene47590 | 0.817339 | 3.47E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene017022 | MS.gene47590 | PPI |
| MS.gene47590 | MS.gene47682 | PPI |
| MS.gene036131 | MS.gene47590 | PPI |
| MS.gene39886 | MS.gene47590 | PPI |
| MS.gene038231 | MS.gene47590 | PPI |
| MS.gene71446 | MS.gene47590 | PPI |
| MS.gene038093 | MS.gene47590 | PPI |
| MS.gene49324 | MS.gene47590 | PPI |
| MS.gene004813 | MS.gene47590 | PPI |
| MS.gene47590 | MS.gene52993 | PPI |
| MS.gene066013 | MS.gene47590 | PPI |
| MS.gene017021 | MS.gene47590 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47590.t1 | MTR_5g090250 | 94.231 | 52 | 3 | 0 | 1 | 52 | 1 | 52 | 2.40e-31 | 103 |
| MS.gene47590.t1 | MTR_5g090250 | 94.231 | 52 | 3 | 0 | 1 | 52 | 1 | 52 | 2.40e-31 | 103 |
| MS.gene47590.t1 | MTR_1g075410 | 94.000 | 50 | 3 | 0 | 3 | 52 | 5 | 54 | 1.62e-28 | 96.3 |
| MS.gene47590.t1 | MTR_1g075340 | 88.462 | 52 | 6 | 0 | 1 | 52 | 1 | 52 | 2.68e-28 | 95.9 |
| MS.gene47590.t1 | MTR_1g075340 | 88.462 | 52 | 6 | 0 | 1 | 52 | 1 | 52 | 2.68e-28 | 95.9 |
| MS.gene47590.t1 | MTR_1g075380 | 82.000 | 50 | 9 | 0 | 3 | 52 | 4 | 53 | 1.65e-22 | 81.3 |
| MS.gene47590.t1 | MTR_1g070335 | 59.615 | 52 | 21 | 0 | 1 | 52 | 1 | 52 | 1.20e-16 | 66.2 |
| MS.gene47590.t1 | MTR_5g045470 | 62.500 | 48 | 18 | 0 | 5 | 52 | 4 | 51 | 5.08e-16 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47590.t1 | AT2G38870 | 63.462 | 52 | 19 | 0 | 1 | 52 | 1 | 52 | 1.76e-18 | 70.9 |
| MS.gene47590.t1 | AT5G43580 | 52.174 | 46 | 22 | 0 | 7 | 52 | 36 | 81 | 9.41e-11 | 52.0 |
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGCCGACTTCCGCTGTGAC+AGG | 0.421191 | 5.1:+71830930 | MS.gene47590:CDS |
| CCTTCCACTCCAACCAACTC+TGG | 0.454716 | 5.1:-71830830 | None:intergenic |
| AGATCCTTCTGGCACAATGA+TGG | 0.502286 | 5.1:-71830900 | None:intergenic |
| CCAGAGTTGGTTGGAGTGGA+AGG | 0.515293 | 5.1:+71830830 | MS.gene47590:CDS |
| GTTGGTTGGAGTGGAAGGAA+AGG | 0.527079 | 5.1:+71830835 | MS.gene47590:CDS |
| GCGGTGACAAAAGATCCTTC+TGG | 0.550057 | 5.1:-71830911 | None:intergenic |
| TATGGTCAGGTAAGAGCTCA+TGG | 0.552544 | 5.1:+71830807 | MS.gene47590:intron |
| CGCCGACTTCCGCTGTGACA+GGG | 0.553611 | 5.1:+71830931 | MS.gene47590:CDS |
| ATGGCCAGAGTTGGTTGGAG+TGG | 0.564909 | 5.1:+71830826 | MS.gene47590:CDS |
| AATTGAGAGGGAGAATCCAT+TGG | 0.570404 | 5.1:+71830871 | MS.gene47590:CDS |
| AACCCTGTCACAGCGGAAGT+CGG | 0.575336 | 5.1:-71830933 | None:intergenic |
| AGCTCATGGCCAGAGTTGGT+TGG | 0.578782 | 5.1:+71830821 | MS.gene47590:CDS |
| TAAGAGCTCATGGCCAGAGT+TGG | 0.585826 | 5.1:+71830817 | MS.gene47590:CDS |
| AGCAGAAGCTACAATTGAGA+GGG | 0.593359 | 5.1:+71830859 | MS.gene47590:CDS |
| TAGCAGAAGCTACAATTGAG+AGG | 0.609404 | 5.1:+71830858 | MS.gene47590:CDS |
| AATGCCATCATTGTGCCAGA+AGG | 0.618440 | 5.1:+71830896 | MS.gene47590:CDS |
| ACAATGATGGCATTCACCAA+TGG | 0.635361 | 5.1:-71830887 | None:intergenic |
| CCTGTCACAGCGGAAGTCGG+CGG | 0.661655 | 5.1:-71830930 | None:intergenic |
| AAAATGTCTGATGAATGCAA+AGG | 0.675933 | 5.1:+71830698 | None:intergenic |
| AAACCCTAACCCTGTCACAG+CGG | 0.735906 | 5.1:-71830940 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATTTTAATTATAATTTGA+TGG | + | chr5.1:71830766-71830785 | MS.gene47590:intron | 10.0% |
| !! | TTGAATTAATCAATATTGTA+TGG | + | chr5.1:71830789-71830808 | MS.gene47590:intron | 15.0% |
| !! | TGTTTAAATTAAATGGTTTG+TGG | + | chr5.1:71830744-71830763 | MS.gene47590:intron | 20.0% |
| ! | TTAATCAATATTGTATGGTC+AGG | + | chr5.1:71830794-71830813 | MS.gene47590:intron | 25.0% |
| AATTGAGAGGGAGAATCCAT+TGG | + | chr5.1:71830871-71830890 | MS.gene47590:CDS | 40.0% | |
| ACAATGATGGCATTCACCAA+TGG | - | chr5.1:71830890-71830909 | None:intergenic | 40.0% | |
| AGCAGAAGCTACAATTGAGA+GGG | + | chr5.1:71830859-71830878 | MS.gene47590:CDS | 40.0% | |
| GTCTGATGAATGCAAAGGTA+AGG | + | chr5.1:71830703-71830722 | MS.gene47590:intron | 40.0% | |
| TAGCAGAAGCTACAATTGAG+AGG | + | chr5.1:71830858-71830877 | MS.gene47590:CDS | 40.0% | |
| AATGCCATCATTGTGCCAGA+AGG | + | chr5.1:71830896-71830915 | MS.gene47590:CDS | 45.0% | |
| AGATCCTTCTGGCACAATGA+TGG | - | chr5.1:71830903-71830922 | None:intergenic | 45.0% | |
| TATGGTCAGGTAAGAGCTCA+TGG | + | chr5.1:71830807-71830826 | MS.gene47590:intron | 45.0% | |
| !! | TATAATATGTTTAAATTAAA+TGG | + | chr5.1:71830737-71830756 | MS.gene47590:intron | 5.0% |
| GCGGTGACAAAAGATCCTTC+TGG | - | chr5.1:71830914-71830933 | None:intergenic | 50.0% | |
| GTTGGTTGGAGTGGAAGGAA+AGG | + | chr5.1:71830835-71830854 | MS.gene47590:CDS | 50.0% | |
| TAAGAGCTCATGGCCAGAGT+TGG | + | chr5.1:71830817-71830836 | MS.gene47590:CDS | 50.0% | |
| CCTTCCACTCCAACCAACTC+TGG | - | chr5.1:71830833-71830852 | None:intergenic | 55.0% | |
| ! | AGCTCATGGCCAGAGTTGGT+TGG | + | chr5.1:71830821-71830840 | MS.gene47590:CDS | 55.0% |
| ! | ATGGCCAGAGTTGGTTGGAG+TGG | + | chr5.1:71830826-71830845 | MS.gene47590:CDS | 55.0% |
| ! | CCAGAGTTGGTTGGAGTGGA+AGG | + | chr5.1:71830830-71830849 | MS.gene47590:CDS | 55.0% |
| CCTGTCACAGCGGAAGTCGG+CGG | - | chr5.1:71830933-71830952 | None:intergenic | 65.0% | |
| !! | CCGCCGACTTCCGCTGTGAC+AGG | + | chr5.1:71830930-71830949 | MS.gene47590:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 71830701 | 71830952 | 71830701 | ID=MS.gene47590 |
| chr5.1 | mRNA | 71830701 | 71830952 | 71830701 | ID=MS.gene47590.t1;Parent=MS.gene47590 |
| chr5.1 | exon | 71830701 | 71830719 | 71830701 | ID=MS.gene47590.t1.exon1;Parent=MS.gene47590.t1 |
| chr5.1 | CDS | 71830701 | 71830719 | 71830701 | ID=cds.MS.gene47590.t1;Parent=MS.gene47590.t1 |
| chr5.1 | exon | 71830816 | 71830952 | 71830816 | ID=MS.gene47590.t1.exon2;Parent=MS.gene47590.t1 |
| chr5.1 | CDS | 71830816 | 71830952 | 71830816 | ID=cds.MS.gene47590.t1;Parent=MS.gene47590.t1 |
| Gene Sequence |
| Protein sequence |
