AlfalfaGEDB | MS.gene49324

M. sativa cultivar XinJiangDaYe / MS.gene49324

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0006950 GO:0008150 GO:0009611 GO:0030234 GO:0030414 GO:0050896 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00280.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49324.t1	KEH42587.1	94.3	70	4	0	1	70	1	70	6.70E-32	146

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49324.t1	P82381	59.1	66	27	0	4	69	3	68	5.7e-16	84.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49324.t1	A0A072VL69	94.3	70	4	0	1	70	1	70	4.8e-32	146.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene49324	MS.gene036131	PPI
MS.gene49324	MS.gene47682	PPI
MS.gene39886	MS.gene49324	PPI
MS.gene49324	MS.gene71446	PPI
MS.gene49324	MS.gene017022	PPI
MS.gene49324	MS.gene038093	PPI
MS.gene038231	MS.gene49324	PPI
MS.gene49324	MS.gene47590	PPI
MS.gene49324	MS.gene066013	PPI
MS.gene49324	MS.gene017021	PPI
MS.gene004813	MS.gene49324	PPI
MS.gene036131	MS.gene49324	PPI
MS.gene49324	MS.gene52993	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49324.t1	MTR_1g070335	94.286	70	4	1	70	1	70	7.96e-44	135
MS.gene49324.t1	MTR_1g070330	81.481	54	10	17	70	1	54	4.30e-26	90.5
MS.gene49324.t1	MTR_1g075410	60.000	70	28	1	70	3	72	3.86e-25	88.6
MS.gene49324.t1	MTR_5g090250	60.000	70	28	1	70	1	70	1.32e-23	84.7
MS.gene49324.t1	MTR_5g090250	60.000	70	28	1	70	1	70	1.32e-23	84.7
MS.gene49324.t1	MTR_1g075340	54.286	70	32	1	70	1	70	1.61e-22	82.0
MS.gene49324.t1	MTR_1g075340	54.286	70	32	1	70	1	70	1.61e-22	82.0
MS.gene49324.t1	MTR_5g045470	55.882	68	30	3	70	2	69	4.27e-21	78.2
MS.gene49324.t1	MTR_1g075380	57.576	66	28	5	70	6	71	2.38e-19	73.9
MS.gene49324.t1	MTR_3g020970	44.444	63	35	8	70	36	98	2.33e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49324.t1	AT2G38870	51.429	70	34	1	70	1	70	3.33e-18	71.2
MS.gene49324.t1	AT2G38900	49.231	65	33	6	70	24	88	1.75e-16	67.4
MS.gene49324.t1	AT2G38900	49.231	65	33	6	70	9	73	2.39e-16	66.6
MS.gene49324.t1	AT5G43580	46.875	64	34	7	70	36	99	2.47e-16	67.0
MS.gene49324.t1	AT3G46860	44.444	63	35	8	70	23	85	1.42e-11	54.7
MS.gene49324.t1	AT5G43570	46.667	60	32	8	67	14	73	1.50e-11	55.1
MS.gene49324.t1	AT5G43570	46.667	60	32	8	67	24	83	2.39e-11	54.7

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCACTAGGGTTTGGGTTT+GGG	0.263801	1.1:-52747626	MS.gene49324:CDS
AATGCACTAGGGTTTGGGTT+TGG	0.268397	1.1:-52747627	MS.gene49324:CDS
ATTTCAAATGCACTAGGGTT+TGG	0.318537	1.1:-52747633	MS.gene49324:CDS
TTTCAAATGCACTAGGGTTT+GGG	0.339535	1.1:-52747632	MS.gene49324:CDS
AAAATGGAAGGTCGATGTTT+AGG	0.365392	1.1:-52747922	MS.gene49324:intron
GAATAAGTGGCCAGAGGTTG+TGG	0.391845	1.1:-52747749	MS.gene49324:CDS
CCGTCCACTCCCACAACCTC+TGG	0.453884	1.1:+52747739	None:intergenic
CAATGGATTTCAAATGCACT+AGG	0.456321	1.1:-52747639	MS.gene49324:CDS
TTACTTTAGGGAAGAATAAG+TGG	0.467450	1.1:-52747762	MS.gene49324:intron
GTGGCCAGAGGTTGTGGGAG+TGG	0.469941	1.1:-52747743	MS.gene49324:CDS
TGGGTTTGGGTCGACAAACA+TGG	0.500413	1.1:-52747613	MS.gene49324:CDS
ATTGTCACAACATGCCCTTC+CGG	0.508925	1.1:+52747658	None:intergenic
CCAGAGGTTGTGGGAGTGGA+CGG	0.522662	1.1:-52747739	MS.gene49324:CDS
AGTGAATGCAATAGTGGTGC+CGG	0.530167	1.1:-52747677	MS.gene49324:CDS
AATAAGTGGCCAGAGGTTGT+GGG	0.533246	1.1:-52747748	MS.gene49324:CDS
GGGTTTGGGTCGACAAACAT+GGG	0.568932	1.1:-52747612	MS.gene49324:CDS
ATGCAATAGTGGTGCCGGAA+GGG	0.587071	1.1:-52747672	MS.gene49324:CDS
TGAGAGAGTGAATGCAATAG+TGG	0.591365	1.1:-52747683	MS.gene49324:CDS
AATGGATTTCAAATGCACTA+GGG	0.595211	1.1:-52747638	MS.gene49324:CDS
AAGCAGAAAAGCAAAATGGA+AGG	0.626986	1.1:-52747934	None:intergenic
AATGCAATAGTGGTGCCGGA+AGG	0.641397	1.1:-52747673	MS.gene49324:CDS
GGAAGGGCATGTTGTGACAA+TGG	0.645412	1.1:-52747656	MS.gene49324:CDS
AGGGAAGAATAAGTGGCCAG+AGG	0.676552	1.1:-52747755	MS.gene49324:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTAATGATTTACTTTA+GGG	-	chr1.1:52747727-52747746	MS.gene49324:CDS	10.0%
!!!	GAATTTTAATGATTTACTTT+AGG	-	chr1.1:52747726-52747745	MS.gene49324:CDS	15.0%
!	GATTGAATGAAAATACTCTA+GGG	-	chr1.1:52747642-52747661	MS.gene49324:CDS	25.0%
!	TGATTGAATGAAAATACTCT+AGG	-	chr1.1:52747641-52747660	MS.gene49324:CDS	25.0%
!	AATGGATTTCAAATGCACTA+GGG	-	chr1.1:52747863-52747882	MS.gene49324:intron	30.0%
!	TTACTTTAGGGAAGAATAAG+TGG	-	chr1.1:52747739-52747758	MS.gene49324:CDS	30.0%
	ATTTCAAATGCACTAGGGTT+TGG	-	chr1.1:52747868-52747887	MS.gene49324:intron	35.0%
	TTTCAAATGCACTAGGGTTT+GGG	-	chr1.1:52747869-52747888	MS.gene49324:intron	35.0%
!	CAATGGATTTCAAATGCACT+AGG	-	chr1.1:52747862-52747881	MS.gene49324:intron	35.0%
	GTTACTCGTGTGCCAAAAAT+AGG	-	chr1.1:52747915-52747934	MS.gene49324:intron	40.0%
	TGAGAGAGTGAATGCAATAG+TGG	-	chr1.1:52747818-52747837	MS.gene49324:intron	40.0%
	AATAAGTGGCCAGAGGTTGT+GGG	-	chr1.1:52747753-52747772	MS.gene49324:CDS	45.0%
	AGTGAATGCAATAGTGGTGC+CGG	-	chr1.1:52747824-52747843	MS.gene49324:intron	45.0%
	ATTGTCACAACATGCCCTTC+CGG	+	chr1.1:52747846-52747865	None:intergenic	45.0%
!	AATGCACTAGGGTTTGGGTT+TGG	-	chr1.1:52747874-52747893	MS.gene49324:intron	45.0%
!	ATGCACTAGGGTTTGGGTTT+GGG	-	chr1.1:52747875-52747894	MS.gene49324:intron	45.0%
	AGGGAAGAATAAGTGGCCAG+AGG	-	chr1.1:52747746-52747765	MS.gene49324:CDS	50.0%
	GAATAAGTGGCCAGAGGTTG+TGG	-	chr1.1:52747752-52747771	MS.gene49324:CDS	50.0%
	GGGTTTGGGTCGACAAACAT+GGG	-	chr1.1:52747889-52747908	MS.gene49324:intron	50.0%
	TGGGTTTGGGTCGACAAACA+TGG	-	chr1.1:52747888-52747907	MS.gene49324:intron	50.0%
!	AATGCAATAGTGGTGCCGGA+AGG	-	chr1.1:52747828-52747847	MS.gene49324:intron	50.0%
!	ATGCAATAGTGGTGCCGGAA+GGG	-	chr1.1:52747829-52747848	MS.gene49324:intron	50.0%
!	GGAAGGGCATGTTGTGACAA+TGG	-	chr1.1:52747845-52747864	MS.gene49324:intron	50.0%
	CCAGAGGTTGTGGGAGTGGA+CGG	-	chr1.1:52747762-52747781	MS.gene49324:intron	60.0%
	CCGTCCACTCCCACAACCTC+TGG	+	chr1.1:52747765-52747784	None:intergenic	65.0%
	GTGGCCAGAGGTTGTGGGAG+TGG	-	chr1.1:52747758-52747777	MS.gene49324:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	52747582	52747941	52747582	ID=MS.gene49324
chr1.1	mRNA	52747582	52747941	52747582	ID=MS.gene49324.t1;Parent=MS.gene49324
chr1.1	exon	52747923	52747941	52747923	ID=MS.gene49324.t1.exon1;Parent=MS.gene49324.t1
chr1.1	CDS	52747923	52747941	52747923	ID=cds.MS.gene49324.t1;Parent=MS.gene49324.t1
chr1.1	exon	52747582	52747775	52747582	ID=MS.gene49324.t1.exon2;Parent=MS.gene49324.t1
chr1.1	CDS	52747582	52747775	52747582	ID=cds.MS.gene49324.t1;Parent=MS.gene49324.t1

Gene Sequence	>MS.gene49324 ATGGAAGGTCGATGTTTAGGTGATCATACATATAATATGCTATATTTTTTAATTTAATTTGATTGAATGAAAATACTCTAGGGATCAAGTTCTATAATCTATAGTTATGATTGTATTATTTCTACATGCATGCTTACTAATATTGAATTTTAATGATTTACTTTAGGGAAGAATAAGTGGCCAGAGGTTGTGGGAGTGGACGGTGATGAAGCAGCAGAGAAGATTGAAAGAGAGAATGAGAGAGTGAATGCAATAGTGGTGCCGGAAGGGCATGTTGTGACAATGGATTTCAAATGCACTAGGGTTTGGGTTTGGGTCGACAAACATGGGAGAGTTACTCGTGTGCCAAAAATAGGATAA
Protein sequence	>MS.gene49324.t1 MEGRCLGKNKWPEVVGVDGDEAAEKIERENERVNAIVVPEGHVVTMDFKCTRVWVWVDKHGRVTRVPKIG