AlfalfaGEDB | MS.gene036131

M. sativa cultivar XinJiangDaYe / MS.gene036131

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0006950 GO:0008150 GO:0009611 GO:0030234 GO:0030414 GO:0050896 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00280.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036131.t1	KEH42587.1	94.3	70	4	0	1	70	1	70	6.70E-32	146

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036131.t1	P82381	59.1	66	27	0	4	69	3	68	5.7e-16	84.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036131.t1	A0A072VL69	94.3	70	4	0	1	70	1	70	4.8e-32	146.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene036131	MS.gene066013	PPI
MS.gene038231	MS.gene036131	PPI
MS.gene036131	MS.gene71446	PPI
MS.gene49324	MS.gene036131	PPI
MS.gene004813	MS.gene036131	PPI
MS.gene036131	MS.gene47590	PPI
MS.gene036131	MS.gene017021	PPI
MS.gene036131	MS.gene52993	PPI
MS.gene036131	MS.gene47682	PPI
MS.gene036131	MS.gene017022	PPI
MS.gene036131	MS.gene038093	PPI
MS.gene39886	MS.gene036131	PPI
MS.gene036131	MS.gene49324	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036131.t1	MTR_1g070335	94.286	70	4	1	70	1	70	7.96e-44	135
MS.gene036131.t1	MTR_1g070330	81.481	54	10	17	70	1	54	4.30e-26	90.5
MS.gene036131.t1	MTR_1g075410	60.000	70	28	1	70	3	72	3.86e-25	88.6
MS.gene036131.t1	MTR_5g090250	60.000	70	28	1	70	1	70	1.32e-23	84.7
MS.gene036131.t1	MTR_5g090250	60.000	70	28	1	70	1	70	1.32e-23	84.7
MS.gene036131.t1	MTR_1g075340	54.286	70	32	1	70	1	70	1.61e-22	82.0
MS.gene036131.t1	MTR_1g075340	54.286	70	32	1	70	1	70	1.61e-22	82.0
MS.gene036131.t1	MTR_5g045470	55.882	68	30	3	70	2	69	4.27e-21	78.2
MS.gene036131.t1	MTR_1g075380	57.576	66	28	5	70	6	71	2.38e-19	73.9
MS.gene036131.t1	MTR_3g020970	44.444	63	35	8	70	36	98	2.33e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036131.t1	AT2G38870	51.429	70	34	1	70	1	70	3.33e-18	71.2
MS.gene036131.t1	AT2G38900	49.231	65	33	6	70	24	88	1.75e-16	67.4
MS.gene036131.t1	AT2G38900	49.231	65	33	6	70	9	73	2.39e-16	66.6
MS.gene036131.t1	AT5G43580	46.875	64	34	7	70	36	99	2.47e-16	67.0
MS.gene036131.t1	AT3G46860	44.444	63	35	8	70	23	85	1.42e-11	54.7
MS.gene036131.t1	AT5G43570	46.667	60	32	8	67	14	73	1.50e-11	55.1
MS.gene036131.t1	AT5G43570	46.667	60	32	8	67	24	83	2.39e-11	54.7

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCACTAGGGTTTGGGTTT+GGG	0.263801	1.3:-50383819	MS.gene036131:CDS
AATGCACTAGGGTTTGGGTT+TGG	0.268397	1.3:-50383820	MS.gene036131:CDS
ATTTCAAATGCACTAGGGTT+TGG	0.318537	1.3:-50383826	MS.gene036131:CDS
TTTCAAATGCACTAGGGTTT+GGG	0.339535	1.3:-50383825	MS.gene036131:CDS
AAAATGGAAGGTCGATGTTT+AGG	0.365392	1.3:-50384115	MS.gene036131:intron
GAATAAGTGGCCAGAGGTTG+TGG	0.391845	1.3:-50383942	MS.gene036131:CDS
CCGTCCACTCCCACAACCTC+TGG	0.453884	1.3:+50383932	None:intergenic
CAATGGATTTCAAATGCACT+AGG	0.456321	1.3:-50383832	MS.gene036131:CDS
TTACTTTAGGGAAGAATAAG+TGG	0.467450	1.3:-50383955	MS.gene036131:intron
GTGGCCAGAGGTTGTGGGAG+TGG	0.469941	1.3:-50383936	MS.gene036131:CDS
TGGGTTTGGGTCGACAAACA+TGG	0.500413	1.3:-50383806	MS.gene036131:CDS
ATTGTCACAACATGCCCTTC+CGG	0.508925	1.3:+50383851	None:intergenic
CCAGAGGTTGTGGGAGTGGA+CGG	0.522662	1.3:-50383932	MS.gene036131:CDS
AGTGAATGCAATAGTGGTGC+CGG	0.530167	1.3:-50383870	MS.gene036131:CDS
AATAAGTGGCCAGAGGTTGT+GGG	0.533246	1.3:-50383941	MS.gene036131:CDS
GGGTTTGGGTCGACAAACAT+GGG	0.568932	1.3:-50383805	MS.gene036131:CDS
ATGCAATAGTGGTGCCGGAA+GGG	0.587071	1.3:-50383865	MS.gene036131:CDS
TGAGAGAGTGAATGCAATAG+TGG	0.591365	1.3:-50383876	MS.gene036131:CDS
AATGGATTTCAAATGCACTA+GGG	0.595211	1.3:-50383831	MS.gene036131:CDS
AAGCAGAAAAGCAAAATGGA+AGG	0.626986	1.3:-50384127	None:intergenic
AATGCAATAGTGGTGCCGGA+AGG	0.641397	1.3:-50383866	MS.gene036131:CDS
GGAAGGGCATGTTGTGACAA+TGG	0.645412	1.3:-50383849	MS.gene036131:CDS
AGGGAAGAATAAGTGGCCAG+AGG	0.676552	1.3:-50383948	MS.gene036131:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTAATGATTTACTTTA+GGG	-	chr1.3:50383920-50383939	MS.gene036131:CDS	10.0%
!!!	GAATTTTAATGATTTACTTT+AGG	-	chr1.3:50383919-50383938	MS.gene036131:CDS	15.0%
!	GATTGAATGAAAATACTCTA+GGG	-	chr1.3:50383835-50383854	MS.gene036131:CDS	25.0%
!	TGATTGAATGAAAATACTCT+AGG	-	chr1.3:50383834-50383853	MS.gene036131:CDS	25.0%
!	AATGGATTTCAAATGCACTA+GGG	-	chr1.3:50384056-50384075	MS.gene036131:intron	30.0%
!	TTACTTTAGGGAAGAATAAG+TGG	-	chr1.3:50383932-50383951	MS.gene036131:CDS	30.0%
	ATTTCAAATGCACTAGGGTT+TGG	-	chr1.3:50384061-50384080	MS.gene036131:intron	35.0%
	TTTCAAATGCACTAGGGTTT+GGG	-	chr1.3:50384062-50384081	MS.gene036131:intron	35.0%
!	CAATGGATTTCAAATGCACT+AGG	-	chr1.3:50384055-50384074	MS.gene036131:intron	35.0%
	GTTACTCGTGTGCCAAAAAT+AGG	-	chr1.3:50384108-50384127	MS.gene036131:intron	40.0%
	TGAGAGAGTGAATGCAATAG+TGG	-	chr1.3:50384011-50384030	MS.gene036131:intron	40.0%
	AATAAGTGGCCAGAGGTTGT+GGG	-	chr1.3:50383946-50383965	MS.gene036131:CDS	45.0%
	AGTGAATGCAATAGTGGTGC+CGG	-	chr1.3:50384017-50384036	MS.gene036131:intron	45.0%
	ATTGTCACAACATGCCCTTC+CGG	+	chr1.3:50384039-50384058	None:intergenic	45.0%
!	AATGCACTAGGGTTTGGGTT+TGG	-	chr1.3:50384067-50384086	MS.gene036131:intron	45.0%
!	ATGCACTAGGGTTTGGGTTT+GGG	-	chr1.3:50384068-50384087	MS.gene036131:intron	45.0%
	AGGGAAGAATAAGTGGCCAG+AGG	-	chr1.3:50383939-50383958	MS.gene036131:CDS	50.0%
	GAATAAGTGGCCAGAGGTTG+TGG	-	chr1.3:50383945-50383964	MS.gene036131:CDS	50.0%
	GGGTTTGGGTCGACAAACAT+GGG	-	chr1.3:50384082-50384101	MS.gene036131:intron	50.0%
	TGGGTTTGGGTCGACAAACA+TGG	-	chr1.3:50384081-50384100	MS.gene036131:intron	50.0%
!	AATGCAATAGTGGTGCCGGA+AGG	-	chr1.3:50384021-50384040	MS.gene036131:intron	50.0%
!	ATGCAATAGTGGTGCCGGAA+GGG	-	chr1.3:50384022-50384041	MS.gene036131:intron	50.0%
!	GGAAGGGCATGTTGTGACAA+TGG	-	chr1.3:50384038-50384057	MS.gene036131:intron	50.0%
	CCAGAGGTTGTGGGAGTGGA+CGG	-	chr1.3:50383955-50383974	MS.gene036131:intron	60.0%
	CCGTCCACTCCCACAACCTC+TGG	+	chr1.3:50383958-50383977	None:intergenic	65.0%
	GTGGCCAGAGGTTGTGGGAG+TGG	-	chr1.3:50383951-50383970	MS.gene036131:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	50383775	50384134	50383775	ID=MS.gene036131
chr1.3	mRNA	50383775	50384134	50383775	ID=MS.gene036131.t1;Parent=MS.gene036131
chr1.3	exon	50384116	50384134	50384116	ID=MS.gene036131.t1.exon1;Parent=MS.gene036131.t1
chr1.3	CDS	50384116	50384134	50384116	ID=cds.MS.gene036131.t1;Parent=MS.gene036131.t1
chr1.3	exon	50383775	50383968	50383775	ID=MS.gene036131.t1.exon2;Parent=MS.gene036131.t1
chr1.3	CDS	50383775	50383968	50383775	ID=cds.MS.gene036131.t1;Parent=MS.gene036131.t1

Gene Sequence	>MS.gene036131 ATGGAAGGTCGATGTTTAGGTGATCATACATATAATATGCTATATTTTTTAATTTAATTTGATTGAATGAAAATACTCTAGGGATCAAGTTCTATAATCTATAGTTATGATTGTATTATTTCTACATGCATGCTTACTAATATTGAATTTTAATGATTTACTTTAGGGAAGAATAAGTGGCCAGAGGTTGTGGGAGTGGACGGTGATGAAGCAGCAGAGAAGATTGAAAGAGAGAATGAGAGAGTGAATGCAATAGTGGTGCCGGAAGGGCATGTTGTGACAATGGATTTCAAATGCACTAGGGTTTGGGTTTGGGTCGACAAACATGGGAGAGTTACTCGTGTGCCAAAAATAGGATAA
Protein sequence	>MS.gene036131.t1 MEGRCLGKNKWPEVVGVDGDEAAEKIERENERVNAIVVPEGHVVTMDFKCTRVWVWVDKHGRVTRVPKIG