AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005060.t1 MTR_1g079790 98.174 657 12 0 23 679 102 758 0.0 1331
MS.gene005060.t1 MTR_1g079790 96.748 492 16 0 23 514 102 593 0.0 982
MS.gene005060.t1 MTR_7g116330 71.298 655 182 5 26 679 112 761 0.0 954
MS.gene005060.t1 MTR_1g073840 55.740 662 276 7 26 679 105 757 0.0 734
MS.gene005060.t1 MTR_1g044210 51.061 660 308 7 26 676 104 757 0.0 654
MS.gene005060.t1 MTR_4g031150 37.026 659 390 13 33 678 137 783 1.93e-132 409
MS.gene005060.t1 MTR_7g109150 36.364 572 320 12 12 564 77 623 3.09e-87 286
MS.gene005060.t1 MTR_4g134880 56.164 73 31 1 608 679 1 73 1.39e-19 84.0
MS.gene005060.t1 MTR_8g080770 33.083 133 79 3 555 679 731 861 3.10e-15 80.1
MS.gene005060.t1 MTR_8g080770 25.595 168 125 0 271 438 262 429 1.23e-12 71.6
MS.gene005060.t1 MTR_8g080770 33.083 133 79 3 555 679 840 970 3.51e-15 80.1
MS.gene005060.t1 MTR_8g080770 25.595 168 125 0 271 438 371 538 1.20e-12 72.0
MS.gene005060.t1 MTR_3g085130 34.921 126 71 3 555 672 695 817 1.14e-13 75.1
MS.gene005060.t1 MTR_3g085130 23.810 168 128 0 271 438 216 383 1.17e-12 71.6
MS.gene005060.t1 MTR_3g085130 34.921 126 71 3 555 672 905 1027 1.77e-13 74.7
MS.gene005060.t1 MTR_3g085130 23.810 168 128 0 271 438 426 593 1.05e-12 72.0
MS.gene005060.t1 MTR_8g075340 23.544 395 246 14 281 636 460 837 2.80e-13 73.9
MS.gene005060.t1 MTR_8g075340 33.077 130 62 3 555 663 1028 1153 7.18e-12 69.3
MS.gene005060.t1 MTR_8g106150 25.000 208 153 1 271 475 362 569 6.61e-13 72.8
MS.gene005060.t1 MTR_8g106150 25.000 208 153 1 271 475 119 326 1.06e-12 72.0
MS.gene005060.t1 MTR_3g105590 26.531 196 128 5 248 438 331 515 4.32e-11 67.0
MS.gene005060.t1 MTR_5g022470 23.650 389 219 13 281 619 498 858 4.36e-11 67.0
MS.gene005060.t1 MTR_5g022470 23.650 389 219 13 281 619 498 858 4.51e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005060.t1 AT3G04580 58.797 665 260 6 26 679 103 764 0.0 750
MS.gene005060.t1 AT3G04580 58.797 665 260 6 26 679 103 764 0.0 750
MS.gene005060.t1 AT3G23150 50.812 677 297 12 24 679 108 769 0.0 612
MS.gene005060.t1 AT3G23150 52.121 495 215 8 24 508 108 590 7.99e-155 461
MS.gene005060.t1 AT1G66340 38.998 659 375 12 31 676 85 729 8.88e-140 426
MS.gene005060.t1 AT1G04310 45.402 522 260 7 29 538 115 623 5.36e-136 414
MS.gene005060.t1 AT1G04310 45.402 522 260 7 29 538 115 623 5.36e-136 414
MS.gene005060.t1 AT2G40940 36.486 518 304 11 33 537 87 592 8.59e-87 285
MS.gene005060.t1 AT5G35750 23.823 361 224 9 272 587 567 921 3.87e-17 86.7
MS.gene005060.t1 AT2G01830 25.568 176 131 0 271 446 428 603 1.53e-15 81.3
MS.gene005060.t1 AT2G01830 25.568 176 131 0 271 446 428 603 1.53e-15 81.3
MS.gene005060.t1 AT2G01830 25.568 176 131 0 271 446 428 603 1.53e-15 81.3
MS.gene005060.t1 AT2G01830 25.568 176 131 0 271 446 428 603 1.53e-15 81.3
MS.gene005060.t1 AT2G01830 25.568 176 131 0 271 446 451 626 1.61e-15 81.3
MS.gene005060.t1 AT2G01830 25.568 176 131 0 271 446 440 615 1.63e-15 81.3
MS.gene005060.t1 AT1G27320 25.134 187 140 0 252 438 410 596 3.06e-14 77.0
MS.gene005060.t1 AT5G10720 23.364 321 207 11 248 552 327 624 3.50e-12 70.5
MS.gene005060.t1 AT2G17820 23.120 359 225 9 282 603 488 832 1.13e-11 68.9

Find 174 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTAATTATGCATATTAT+TGG 0.179884 1.2:+61934945 MS.gene005060:CDS
CCCTCTCCTTCTTAGAATTA+AGG 0.206742 1.2:+61933879 MS.gene005060:CDS
GGATATCTCTGAAAATAAAA+AGG 0.209690 1.2:+61934761 MS.gene005060:CDS
TGTGAATTTGAATAATCTTT+AGG 0.255453 1.2:-61936596 None:intergenic
TTCATCAGGGTTTGAGTGCC+TGG 0.258763 1.2:+61936718 MS.gene005060:CDS
GATGAAGGAGGATTGGTTTC+CGG 0.271626 1.2:-61936940 None:intergenic
GCTATGATCAAAGGCCAATT+AGG 0.272954 1.2:-61936851 None:intergenic
TGTTGAGCTTTCTAAGGCTT+TGG 0.281832 1.2:+61934053 MS.gene005060:CDS
TACTCTTGTTGAGCTTTCTA+AGG 0.286989 1.2:+61934047 MS.gene005060:CDS
TGCACCAAACCCTGAGAGTT+TGG 0.309721 1.2:-61936512 None:intergenic
TTTATGTCTACATACTCAAC+TGG 0.311908 1.2:-61935077 None:intergenic
GGAAATTCAATGGCCCTAAT+TGG 0.315301 1.2:+61936837 MS.gene005060:CDS
GGATGCTTACTAGGGAAATT+AGG 0.316546 1.2:+61933992 MS.gene005060:CDS
ATGTTATGCTATACTAGTTT+TGG 0.328451 1.2:+61934374 MS.gene005060:CDS
GATTATTCAAATTCACAGTT+TGG 0.328563 1.2:+61936602 MS.gene005060:CDS
AGGAAGCTAGTTGGCATGTT+AGG 0.342538 1.2:+61933971 MS.gene005060:CDS
AGGACTGAACAAAAGATTAT+TGG 0.343143 1.2:+61934684 MS.gene005060:CDS
CAGATGATGATAGTTTAAAT+AGG 0.352906 1.2:+61936645 MS.gene005060:CDS
ACTTCAAAACCATCCATTTC+AGG 0.357064 1.2:-61936800 None:intergenic
ATCTGTTGTTTCGTTGAAAT+CGG 0.358826 1.2:-61935140 None:intergenic
ATTTGCATAATTGTGCTGTT+TGG 0.362682 1.2:+61934076 MS.gene005060:CDS
TTCCTTATCTCCAACACATC+AGG 0.366420 1.2:-61934195 None:intergenic
TTCTTCCAGGTTCGGACTCT+AGG 0.375382 1.2:+61934397 MS.gene005060:CDS
CTAGTAACAGCAGTTTCATC+AGG 0.380902 1.2:+61936704 MS.gene005060:CDS
CAGGTTCGGACTCTAGGGTT+TGG 0.381488 1.2:+61934403 MS.gene005060:CDS
GGCATGTTAGGATGCTTACT+AGG 0.396870 1.2:+61933983 MS.gene005060:CDS
TGGAATTGGGGCAAGAAGTT+GGG 0.397658 1.2:+61933932 MS.gene005060:CDS
TCATGAATTGAAATCGGATT+CGG 0.398997 1.2:+61934134 MS.gene005060:CDS
TTTGACTCATGAATTGAAAT+CGG 0.399140 1.2:+61934128 MS.gene005060:CDS
GAATCACAACAGTGAATCTA+AGG 0.402957 1.2:+61935175 MS.gene005060:CDS
ATGGAATTGGGGCAAGAAGT+TGG 0.404627 1.2:+61933931 MS.gene005060:CDS
GTCCATGGCTCATCACTGTT+TGG 0.409431 1.2:-61934602 None:intergenic
CCCTCAACATGGAATGTAGA+AGG 0.409888 1.2:-61934812 None:intergenic
CATGAATTGAAATCGGATTC+GGG 0.409935 1.2:+61934135 MS.gene005060:CDS
AGATAATGCAAGGTAATATA+TGG 0.410537 1.2:+61936483 MS.gene005060:intron
AGGCAGAATGTGATGGAATT+GGG 0.410985 1.2:+61933919 MS.gene005060:CDS
AGTTGAGGTGGTGGCTGATC+AGG 0.412583 1.2:+61934446 MS.gene005060:CDS
GAGGCAGAATGTGATGGAAT+TGG 0.412904 1.2:+61933918 MS.gene005060:CDS
TTGAAGTGGCGGCAAGGATC+AGG 0.415267 1.2:+61936816 MS.gene005060:CDS
ATTACCAAACTCTCAGGGTT+TGG 0.429450 1.2:+61936508 MS.gene005060:CDS
ATGGATATTACCAAACTCTC+AGG 0.429769 1.2:+61936502 MS.gene005060:CDS
AGCATACACACAATCCTTGT+TGG 0.430934 1.2:+61933589 MS.gene005060:CDS
CCTTCTACATTCCATGTTGA+GGG 0.431332 1.2:+61934812 MS.gene005060:CDS
ATCTCTCTCCTATCATCATT+CGG 0.433427 1.2:-61934102 None:intergenic
ACCTCAACTATCTCCATCTC+TGG 0.436575 1.2:-61934432 None:intergenic
TTGTGTGTGTATCAAGGGTT+TGG 0.438334 1.2:+61934858 MS.gene005060:CDS
GCAACTCCTAGTTTAGATTC+AGG 0.441706 1.2:-61934243 None:intergenic
TTTGAGACTGTTGCAATGCT+CGG 0.446015 1.2:-61934542 None:intergenic
CAAACCCTAGAGTCCGAACC+TGG 0.447027 1.2:-61934402 None:intergenic
GAAAGCAAGAGTTGAAAAGA+AGG 0.447107 1.2:-61933787 None:intergenic
TAAGGAAAACTAAAGGGGTT+AGG 0.448583 1.2:+61934211 MS.gene005060:CDS
CTATGATCAAAGGCCAATTA+GGG 0.451338 1.2:-61936850 None:intergenic
CTGGGTGCTATAAGTGGCTC+TGG 0.456114 1.2:+61936737 MS.gene005060:CDS
CTTCTTCTAAAGTTTCAGAT+AGG 0.459490 1.2:+61936545 MS.gene005060:CDS
TACAAGTGAGGGTGGTGATA+AGG 0.459678 1.2:+61935019 MS.gene005060:CDS
GAACGCGATGATGGCCAGCC+AGG 0.462578 1.2:+61934566 MS.gene005060:CDS
TGGAGATAAGGAAAACTAAA+GGG 0.470604 1.2:+61934205 MS.gene005060:CDS
TAATGCAGAAGAGCAAGTTA+AGG 0.472874 1.2:+61936883 MS.gene005060:CDS
AGACATTCACATGCCTGAAA+TGG 0.474093 1.2:+61936787 MS.gene005060:CDS
TCCTTCTACATTCCATGTTG+AGG 0.474448 1.2:+61934811 MS.gene005060:CDS
GTTGCGAGTAGTGGCGGTGG+GGG 0.474504 1.2:+61934261 MS.gene005060:CDS
GGAGTTGCGAGTAGTGGCGG+TGG 0.474808 1.2:+61934258 MS.gene005060:CDS
CAAAGGAGGGACGCCGGAGT+TGG 0.475152 1.2:+61934344 MS.gene005060:CDS
CTTCATGTTTCGAATTTCAA+AGG 0.475335 1.2:+61934327 MS.gene005060:CDS
AAAGAAGCTACTCGAGAGGC+TGG 0.477400 1.2:+61936676 MS.gene005060:CDS
GAGTTGCGAGTAGTGGCGGT+GGG 0.478888 1.2:+61934259 MS.gene005060:CDS
CGAGAACCGACGCGTGAGAA+AGG 0.484376 1.2:-61934479 None:intergenic
ACCCTGATGTGTTGGAGATA+AGG 0.484829 1.2:+61934193 MS.gene005060:CDS
GAATGACTTCCTAGCCTGGC+TGG 0.485805 1.2:-61934580 None:intergenic
TCATCAGGGTTTGAGTGCCT+GGG 0.491049 1.2:+61936719 MS.gene005060:CDS
TCTTCCAGGTTCGGACTCTA+GGG 0.492992 1.2:+61934398 MS.gene005060:CDS
AAGTGCTTGTGTGTGTATCA+AGG 0.498129 1.2:+61934852 MS.gene005060:CDS
TGGGATTATGAAGAAACAAA+AGG 0.498382 1.2:+61933951 MS.gene005060:CDS
TTTGGAATGACTTCCTAGCC+TGG 0.501343 1.2:-61934584 None:intergenic
TTTGGATGCCGAATGATGAT+AGG 0.501945 1.2:+61934094 MS.gene005060:CDS
TTGGAGATAAGGAAAACTAA+AGG 0.504670 1.2:+61934204 MS.gene005060:CDS
TTGAGGCCTGAATCTAAACT+AGG 0.504811 1.2:+61934237 MS.gene005060:CDS
TGAGGAATTTAGCAACAGTA+AGG 0.504921 1.2:-61933810 None:intergenic
AGGCCAATGCATTCAGTTGT+GGG 0.505607 1.2:+61934630 MS.gene005060:CDS
CCCTCACCTTAATTCTAAGA+AGG 0.509242 1.2:-61933885 None:intergenic
CGATGATGGCCAGCCAGGCT+AGG 0.509807 1.2:+61934571 MS.gene005060:CDS
GAGTTTAGTAAACGATGTAA+TGG 0.515957 1.2:+61934740 MS.gene005060:CDS
AACTCTCAGGGTTTGGTGCA+AGG 0.516591 1.2:+61936515 MS.gene005060:CDS
GAGTGCCTGGGTGCTATAAG+TGG 0.519942 1.2:+61936731 MS.gene005060:CDS
GCATTGGCCTCCTCAGTCCA+TGG 0.520880 1.2:-61934617 None:intergenic
AAGGATAGATGCATGCTGGC+TGG 0.523016 1.2:+61936902 MS.gene005060:CDS
GTCTCAAAAGAACGCGATGA+TGG 0.526187 1.2:+61934557 MS.gene005060:CDS
TTTCTTGAGGCAGAATGTGA+TGG 0.526914 1.2:+61933912 MS.gene005060:CDS
GAGAACCGACGCGTGAGAAA+GGG 0.532015 1.2:-61934478 None:intergenic
TGTTGAACACGCGTGACCGA+GGG 0.532022 1.2:+61934973 MS.gene005060:CDS
GCTATCTGATGAAGGAGGAT+TGG 0.533500 1.2:-61936947 None:intergenic
TGTGATTGACCCTGATGTGT+TGG 0.534459 1.2:+61934185 MS.gene005060:CDS
TATGTGCCGGAGGTTGGTAC+AGG 0.536453 1.2:+61935214 MS.gene005060:CDS
GCAAGGATCAGGAAATTCAA+TGG 0.537123 1.2:+61936827 MS.gene005060:CDS
CTTCAATATGTGCCGGAGGT+TGG 0.538586 1.2:+61935208 MS.gene005060:CDS
GCTATAAGAGCTATGATCAA+AGG 0.539373 1.2:-61936860 None:intergenic
CAACGAAACAACAGATTGGT+AGG 0.539988 1.2:+61935147 MS.gene005060:CDS
GAGGCCAATGCATTCAGTTG+TGG 0.544582 1.2:+61934629 MS.gene005060:CDS
TTGTTGAACACGCGTGACCG+AGG 0.544947 1.2:+61934972 MS.gene005060:CDS
GGTTTGGACCGCTCCAGAGA+TGG 0.547219 1.2:+61934419 MS.gene005060:CDS
ATTTCAACGAAACAACAGAT+TGG 0.547312 1.2:+61935143 MS.gene005060:CDS
TGTTTCAAGAGGGTAATATG+AGG 0.554581 1.2:+61934664 MS.gene005060:CDS
AACATGTATCTTTCAAGCGA+TGG 0.555713 1.2:-61936569 None:intergenic
TGCATGCTGGCTGGAATGAA+TGG 0.556631 1.2:+61936911 MS.gene005060:CDS
GTTCATCCGCTATCTGATGA+AGG 0.557017 1.2:-61936955 None:intergenic
AGTGCTTGTGTGTGTATCAA+GGG 0.557402 1.2:+61934853 MS.gene005060:CDS
CTGGAATGAATGGTGTGATC+CGG 0.559043 1.2:+61936921 MS.gene005060:CDS
AGTTGCGAGTAGTGGCGGTG+GGG 0.561735 1.2:+61934260 MS.gene005060:CDS
ATGTTTCGAATTTCAAAGGA+GGG 0.563184 1.2:+61934331 MS.gene005060:CDS
GAATTTCAAAGGAGGGACGC+CGG 0.563898 1.2:+61934338 MS.gene005060:CDS
CACCTACCTGTACCAACCTC+CGG 0.564583 1.2:-61935220 None:intergenic
AACACATCAGGGTCAATCAC+AGG 0.564986 1.2:-61934183 None:intergenic
AACATGGAATGTAGAAGGAA+AGG 0.568366 1.2:-61934807 None:intergenic
CGTAGCCCTTTCTCACGCGT+CGG 0.568817 1.2:+61934473 MS.gene005060:CDS
AAATTCGAAACATGAAGCAT+CGG 0.568829 1.2:-61934321 None:intergenic
TATTCAAATTCACAGTTTGG+AGG 0.570060 1.2:+61936605 MS.gene005060:CDS
ATCCCCACAACTGAATGCAT+TGG 0.570182 1.2:-61934633 None:intergenic
CATGTTTCGAATTTCAAAGG+AGG 0.571827 1.2:+61934330 MS.gene005060:CDS
GCATGTTAGGATGCTTACTA+GGG 0.572208 1.2:+61933984 MS.gene005060:CDS
AGATCGGTCCTACTTCGTGC+AGG 0.573170 1.2:+61936980 MS.gene005060:CDS
TGCTCTTGTGTCGTGTGCAA+CGG 0.573831 1.2:+61933834 MS.gene005060:CDS
AGAAACAAAAGGAAGCTAGT+TGG 0.575842 1.2:+61933962 MS.gene005060:CDS
ACCTTAATTCTAAGAAGGAG+AGG 0.576433 1.2:-61933880 None:intergenic
ATTCACATGCCTGAAATGGA+TGG 0.579300 1.2:+61936791 MS.gene005060:CDS
TTCCAAACAGTGATGAGCCA+TGG 0.580133 1.2:+61934600 MS.gene005060:CDS
CCTTAATTCTAAGAAGGAGA+GGG 0.581303 1.2:-61933879 None:intergenic
TCCTTCTTAGAATTAAGGTG+AGG 0.582740 1.2:+61933884 MS.gene005060:CDS
CCTTCTTAGAATTAAGGTGA+GGG 0.583317 1.2:+61933885 MS.gene005060:CDS
GCTTGAGCTTCAATATGTGC+CGG 0.584592 1.2:+61935201 MS.gene005060:CDS
TCCTTATCTCCAACACATCA+GGG 0.586059 1.2:-61934194 None:intergenic
AACTATCTCCATCTCTGGAG+CGG 0.592322 1.2:-61934427 None:intergenic
TGAGGAGTTGGGTGCTGTCG+CGG 0.592472 1.2:+61934287 MS.gene005060:CDS
AGTTAAGGATAGATGCATGC+TGG 0.598062 1.2:+61936898 MS.gene005060:CDS
AAGGTTTCATCTCTAACTGA+AGG 0.599231 1.2:-61934788 None:intergenic
TGGATATTACCAAACTCTCA+GGG 0.599355 1.2:+61936503 MS.gene005060:CDS
GGAGATAAGGAAAACTAAAG+GGG 0.605752 1.2:+61934206 MS.gene005060:CDS
ATGCTTTCATTGTTTCAAGA+GGG 0.605913 1.2:+61934654 MS.gene005060:CDS
AAACTAGGAGTTGCGAGTAG+TGG 0.606504 1.2:+61934252 MS.gene005060:CDS
CTAGGAGTTGCGAGTAGTGG+CGG 0.609987 1.2:+61934255 MS.gene005060:CDS
AAGAAGCTACTCGAGAGGCT+GGG 0.610653 1.2:+61936677 MS.gene005060:CDS
GGTGCTATAAGTGGCTCTGG+TGG 0.610827 1.2:+61936740 MS.gene005060:CDS
TAGTAACAGCAGTTTCATCA+GGG 0.611000 1.2:+61936705 MS.gene005060:CDS
AATCTTATGCAGATAATGCA+AGG 0.616623 1.2:+61936473 MS.gene005060:intron
GAGGAATTTAGCAACAGTAA+GGG 0.621503 1.2:-61933809 None:intergenic
GATGCTTTCATTGTTTCAAG+AGG 0.621734 1.2:+61934653 MS.gene005060:CDS
GATGGAGATAGTTGAGGTGG+TGG 0.622877 1.2:+61934437 MS.gene005060:CDS
TCACAACAGTGAATCTAAGG+AGG 0.625084 1.2:+61935178 MS.gene005060:CDS
GATAGCGGATGAACTTAGAT+CGG 0.629033 1.2:+61936964 MS.gene005060:CDS
GCAAGCGGCTTCCCTCAACA+TGG 0.629945 1.2:-61934823 None:intergenic
CAAGCACTTAGCAATGCAAG+CGG 0.631343 1.2:-61934838 None:intergenic
GGTGACACAATGTTGAAAGT+CGG 0.632956 1.2:+61934705 MS.gene005060:CDS
CTAAGAAGGAGAGGGATCAA+CGG 0.639905 1.2:-61933871 None:intergenic
TAACATGTATCAACCAACTC+CGG 0.640709 1.2:-61934357 None:intergenic
CACAACAGTGAATCTAAGGA+GGG 0.640846 1.2:+61935179 MS.gene005060:CDS
TCCAGAGATGGAGATAGTTG+AGG 0.641469 1.2:+61934431 MS.gene005060:CDS
GGCCAATGCATTCAGTTGTG+GGG 0.642247 1.2:+61934631 MS.gene005060:CDS
TGAGCTTCAATATGTGCCGG+AGG 0.643404 1.2:+61935204 MS.gene005060:CDS
AGAGATGGAGATAGTTGAGG+TGG 0.648766 1.2:+61934434 MS.gene005060:CDS
GGCAGAATGTGATGGAATTG+GGG 0.651385 1.2:+61933920 MS.gene005060:CDS
TTATGTCTACATACTCAACT+GGG 0.652246 1.2:-61935076 None:intergenic
CAGTGATGAGCCATGGACTG+AGG 0.655532 1.2:+61934607 MS.gene005060:CDS
TGAGGTGGTGGCTGATCAGG+TGG 0.658305 1.2:+61934449 MS.gene005060:CDS
CGAAACAACAGATTGGTAGG+AGG 0.659295 1.2:+61935150 MS.gene005060:CDS
CATCTGCTAGCAAAATCTTG+AGG 0.670396 1.2:-61936628 None:intergenic
CCACTGTAACTGTGATGATG+AGG 0.670665 1.2:+61933555 MS.gene005060:CDS
TGATGAGCCATGGACTGAGG+AGG 0.672113 1.2:+61934610 MS.gene005060:CDS
CCTCATCATCACAGTTACAG+TGG 0.677470 1.2:-61933555 None:intergenic
CAGAGCCACTTATAGCACCC+AGG 0.678501 1.2:-61936736 None:intergenic
CATCCGCTATCTGATGAAGG+AGG 0.680590 1.2:-61936952 None:intergenic
CGACGCGTGAGAAAGGGCTA+CGG 0.681594 1.2:-61934472 None:intergenic
AATCCTCCTTCATCAGATAG+CGG 0.688493 1.2:+61936949 MS.gene005060:CDS
TGACAAAGAAGCTACTCGAG+AGG 0.707649 1.2:+61936672 MS.gene005060:CDS
AGAAGGAGAGGGATCAACGG+CGG 0.710786 1.2:-61933868 None:intergenic
CACACGACACAAGAGCAGTG+AGG 0.767368 1.2:-61933828 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTAAATAATTACACA+GGG - chr1.2:61936408-61936427 None:intergenic 10.0%
!! TGTAATTATTTAATTAATCA+TGG + chr1.2:61936410-61936429 MS.gene005060:intron 10.0%
!! ATTATAAGTATAAAGAAGTA+AGG - chr1.2:61936273-61936292 None:intergenic 15.0%
!! GATTAATTAAATAATTACAC+AGG - chr1.2:61936409-61936428 None:intergenic 15.0%
!! TAGTGCTTTAATAAAATAAT+TGG - chr1.2:61936301-61936320 None:intergenic 15.0%
!! CAAGTAATTATGCATATTAT+TGG + chr1.2:61934945-61934964 MS.gene005060:CDS 20.0%
!! TGTGAATTTGAATAATCTTT+AGG - chr1.2:61936599-61936618 None:intergenic 20.0%
!! TTTCTTATGTTGAAAGATTT+AGG - chr1.2:61935792-61935811 None:intergenic 20.0%
!!! AAATTTGATTTTCAGATTAC+TGG + chr1.2:61935098-61935117 MS.gene005060:CDS 20.0%
!!! AATTTTACATCCTTAATGAA+TGG - chr1.2:61935460-61935479 None:intergenic 20.0%
!!! CTTTTATCTTAATAGTATCA+CGG + chr1.2:61935848-61935867 MS.gene005060:intron 20.0%
!!! GATATCTCTGAAAATAAAAA+GGG + chr1.2:61934762-61934781 MS.gene005060:CDS 20.0%
!!! TAATTTTGTTTTCCTCTTTT+AGG + chr1.2:61935938-61935957 MS.gene005060:intron 20.0%
! AATGCTTAAAGAATGATGTT+TGG + chr1.2:61935335-61935354 MS.gene005060:intron 25.0%
! AGATAATGCAAGGTAATATA+TGG + chr1.2:61936483-61936502 MS.gene005060:intron 25.0%
! ATGACAAAAAAATTGGTGTA+TGG + chr1.2:61935042-61935061 MS.gene005060:CDS 25.0%
! CACTGTCTATATTATATATG+TGG + chr1.2:61935246-61935265 MS.gene005060:intron 25.0%
! CAGATGATGATAGTTTAAAT+AGG + chr1.2:61936645-61936664 MS.gene005060:CDS 25.0%
! CGTGATACTATTAAGATAAA+AGG - chr1.2:61935850-61935869 None:intergenic 25.0%
! GATAAGGATGACAAAAAAAT+TGG + chr1.2:61935035-61935054 MS.gene005060:CDS 25.0%
! GATTATTCAAATTCACAGTT+TGG + chr1.2:61936602-61936621 MS.gene005060:CDS 25.0%
! TGAAGAAACAACAATTTGAA+AGG - chr1.2:61933700-61933719 None:intergenic 25.0%
! TTTCTTCAAACCATTCATTA+AGG + chr1.2:61935447-61935466 MS.gene005060:intron 25.0%
! TTTGACTCATGAATTGAAAT+CGG + chr1.2:61934128-61934147 MS.gene005060:CDS 25.0%
!! AAAGATTTAGGTTATAACAG+AGG - chr1.2:61935780-61935799 None:intergenic 25.0%
!! GGATATCTCTGAAAATAAAA+AGG + chr1.2:61934761-61934780 MS.gene005060:CDS 25.0%
!! TTCTGACTTTATTTACATTG+TGG + chr1.2:61936450-61936469 MS.gene005060:intron 25.0%
!!! ATGTTATGCTATACTAGTTT+TGG + chr1.2:61934374-61934393 MS.gene005060:CDS 25.0%
!!! TTTTGTAGTCTTGTTCATAT+TGG - chr1.2:61936105-61936124 None:intergenic 25.0%
AAATTCGAAACATGAAGCAT+CGG - chr1.2:61934324-61934343 None:intergenic 30.0%
AATCTTATGCAGATAATGCA+AGG + chr1.2:61936473-61936492 MS.gene005060:intron 30.0%
ACAAAGTAAAGAAGCTCAAT+TGG - chr1.2:61933661-61933680 None:intergenic 30.0%
ACCTATTTCCATTGATGAAA+AGG - chr1.2:61936138-61936157 None:intergenic 30.0%
AGACATAACATAGACAATCA+AGG - chr1.2:61935282-61935301 None:intergenic 30.0%
AGGACTGAACAAAAGATTAT+TGG + chr1.2:61934684-61934703 MS.gene005060:CDS 30.0%
ATGCTTTCATTGTTTCAAGA+GGG + chr1.2:61934654-61934673 MS.gene005060:CDS 30.0%
ATGTTTCGAATTTCAAAGGA+GGG + chr1.2:61934331-61934350 MS.gene005060:CDS 30.0%
ATTTCAACGAAACAACAGAT+TGG + chr1.2:61935143-61935162 MS.gene005060:CDS 30.0%
CTTCATGTTTCGAATTTCAA+AGG + chr1.2:61934327-61934346 MS.gene005060:CDS 30.0%
GAGTTTAGTAAACGATGTAA+TGG + chr1.2:61934740-61934759 MS.gene005060:CDS 30.0%
TATTCAAATTCACAGTTTGG+AGG + chr1.2:61936605-61936624 MS.gene005060:CDS 30.0%
TCATGAATTGAAATCGGATT+CGG + chr1.2:61934134-61934153 MS.gene005060:CDS 30.0%
TGGAGATAAGGAAAACTAAA+GGG + chr1.2:61934205-61934224 MS.gene005060:CDS 30.0%
TGGGATTATGAAGAAACAAA+AGG + chr1.2:61933951-61933970 MS.gene005060:CDS 30.0%
TTATGTCTACATACTCAACT+GGG - chr1.2:61935079-61935098 None:intergenic 30.0%
TTGGAGATAAGGAAAACTAA+AGG + chr1.2:61934204-61934223 MS.gene005060:CDS 30.0%
TTGTGTGATGTAAACTCAAA+AGG - chr1.2:61935905-61935924 None:intergenic 30.0%
TTTATGTCTACATACTCAAC+TGG - chr1.2:61935080-61935099 None:intergenic 30.0%
! AAGAAACTGATTCTACGAAT+CGG + chr1.2:61935618-61935637 MS.gene005060:intron 30.0%
! ATAAGAAAGCTTGTGCTTTT+TGG + chr1.2:61936163-61936182 MS.gene005060:intron 30.0%
! ATCTCTGAAAATAAAAAGGG+TGG + chr1.2:61934765-61934784 MS.gene005060:CDS 30.0%
! ATCTTTTGTGCCTTAAAATC+TGG + chr1.2:61936237-61936256 MS.gene005060:intron 30.0%
! ATTTGCATAATTGTGCTGTT+TGG + chr1.2:61934076-61934095 MS.gene005060:CDS 30.0%
! GAATAACAAGTTCTGATTTG+TGG - chr1.2:61936346-61936365 None:intergenic 30.0%
! GTGTCTTTTCTTGAATTGTA+AGG + chr1.2:61936057-61936076 MS.gene005060:intron 30.0%
! TCCTACTTTACCAGATTTTA+AGG - chr1.2:61936250-61936269 None:intergenic 30.0%
! TCCTTTTCATCAATGGAAAT+AGG + chr1.2:61936134-61936153 MS.gene005060:intron 30.0%
! TCTTTGCCTGAAAAAGTTTT+AGG + chr1.2:61934903-61934922 MS.gene005060:CDS 30.0%
!! ATCTGTTGTTTCGTTGAAAT+CGG - chr1.2:61935143-61935162 None:intergenic 30.0%
!! CTTCTTCTAAAGTTTCAGAT+AGG + chr1.2:61936545-61936564 MS.gene005060:CDS 30.0%
!! TAAGAAAGCTTGTGCTTTTT+GGG + chr1.2:61936164-61936183 MS.gene005060:intron 30.0%
AAAAAAGAGTCTCGACACAA+CGG - chr1.2:61935680-61935699 None:intergenic 35.0%
AACATGGAATGTAGAAGGAA+AGG - chr1.2:61934810-61934829 None:intergenic 35.0%
AACATGTATCTTTCAAGCGA+TGG - chr1.2:61936572-61936591 None:intergenic 35.0%
AAGGTTTCATCTCTAACTGA+AGG - chr1.2:61934791-61934810 None:intergenic 35.0%
AATCAGTTTCTTGCAATTGC+AGG - chr1.2:61935610-61935629 None:intergenic 35.0%
ACTTCAAAACCATCCATTTC+AGG - chr1.2:61936803-61936822 None:intergenic 35.0%
AGAAACAAAAGGAAGCTAGT+TGG + chr1.2:61933962-61933981 MS.gene005060:CDS 35.0%
ATCAGTTTCTTGCAATTGCA+GGG - chr1.2:61935609-61935628 None:intergenic 35.0%
ATCTCTCTCCTATCATCATT+CGG - chr1.2:61934105-61934124 None:intergenic 35.0%
ATGGATATTACCAAACTCTC+AGG + chr1.2:61936502-61936521 MS.gene005060:CDS 35.0%
CATACCACATAGAACTATGA+AGG - chr1.2:61933731-61933750 None:intergenic 35.0%
CATGAATTGAAATCGGATTC+GGG + chr1.2:61934135-61934154 MS.gene005060:CDS 35.0%
CATGTTTCGAATTTCAAAGG+AGG + chr1.2:61934330-61934349 MS.gene005060:CDS 35.0%
CCTTCTTAGAATTAAGGTGA+GGG + chr1.2:61933885-61933904 MS.gene005060:CDS 35.0%
CTATGATCAAAGGCCAATTA+GGG - chr1.2:61936853-61936872 None:intergenic 35.0%
GAAAGCAAGAGTTGAAAAGA+AGG - chr1.2:61933790-61933809 None:intergenic 35.0%
GAATCACAACAGTGAATCTA+AGG + chr1.2:61935175-61935194 MS.gene005060:CDS 35.0%
GAGGAATTTAGCAACAGTAA+GGG - chr1.2:61933812-61933831 None:intergenic 35.0%
GATGCTTTCATTGTTTCAAG+AGG + chr1.2:61934653-61934672 MS.gene005060:CDS 35.0%
GCAATAACTGATCTCATATG+AGG + chr1.2:61935536-61935555 MS.gene005060:intron 35.0%
GCCTTAAAATCTGGTAAAGT+AGG + chr1.2:61936246-61936265 MS.gene005060:intron 35.0%
GCTATAAGAGCTATGATCAA+AGG - chr1.2:61936863-61936882 None:intergenic 35.0%
GCTCTTAGCTGAAATTAGTA+TGG - chr1.2:61935747-61935766 None:intergenic 35.0%
GGAGATAAGGAAAACTAAAG+GGG + chr1.2:61934206-61934225 MS.gene005060:CDS 35.0%
TAACATGTATCAACCAACTC+CGG - chr1.2:61934360-61934379 None:intergenic 35.0%
TAAGGAAAACTAAAGGGGTT+AGG + chr1.2:61934211-61934230 MS.gene005060:CDS 35.0%
TAATGCAGAAGAGCAAGTTA+AGG + chr1.2:61936883-61936902 MS.gene005060:CDS 35.0%
TACTCTTGTTGAGCTTTCTA+AGG + chr1.2:61934047-61934066 MS.gene005060:CDS 35.0%
TAGTAACAGCAGTTTCATCA+GGG + chr1.2:61936705-61936724 MS.gene005060:CDS 35.0%
TCCTTCTTAGAATTAAGGTG+AGG + chr1.2:61933884-61933903 MS.gene005060:CDS 35.0%
TGAGGAATTTAGCAACAGTA+AGG - chr1.2:61933813-61933832 None:intergenic 35.0%
TGGATATTACCAAACTCTCA+GGG + chr1.2:61936503-61936522 MS.gene005060:CDS 35.0%
TGTTTCAAGAGGGTAATATG+AGG + chr1.2:61934664-61934683 MS.gene005060:CDS 35.0%
! ACCTTAATTCTAAGAAGGAG+AGG - chr1.2:61933883-61933902 None:intergenic 35.0%
! ATAGCCTTCATAGTTCTATG+TGG + chr1.2:61933724-61933743 MS.gene005060:intron 35.0%
! CCTTAATTCTAAGAAGGAGA+GGG - chr1.2:61933882-61933901 None:intergenic 35.0%
! GATTTAGGTTATAACAGAGG+AGG - chr1.2:61935777-61935796 None:intergenic 35.0%
! TCTCACTTCCTTTTCATCAA+TGG + chr1.2:61936127-61936146 MS.gene005060:intron 35.0%
!! AAGAAAGCTTGTGCTTTTTG+GGG + chr1.2:61936165-61936184 MS.gene005060:intron 35.0%
!! TCATCGCCTAAAACTTTTTC+AGG - chr1.2:61934912-61934931 None:intergenic 35.0%
!!! ATACTAGTTTTGGTTCTTCC+AGG + chr1.2:61934384-61934403 MS.gene005060:CDS 35.0%
!!! TGAAATGGATGGTTTTGAAG+TGG + chr1.2:61936802-61936821 MS.gene005060:CDS 35.0%
AAAAGGATCCTTCCAACATG+GGG - chr1.2:61935888-61935907 None:intergenic 40.0%
AAAGCAGATTCTGACATGAC+AGG + chr1.2:61935982-61936001 MS.gene005060:intron 40.0%
AAGTGCTTGTGTGTGTATCA+AGG + chr1.2:61934852-61934871 MS.gene005060:CDS 40.0%
AATCCTCCTTCATCAGATAG+CGG + chr1.2:61936949-61936968 MS.gene005060:CDS 40.0%
ACACAGAAACTGCATGATTG+TGG + chr1.2:61936035-61936054 MS.gene005060:intron 40.0%
ACCAATAACTGATCTGTTGC+AGG + chr1.2:61935575-61935594 MS.gene005060:intron 40.0%
AGACATTCACATGCCTGAAA+TGG + chr1.2:61936787-61936806 MS.gene005060:CDS 40.0%
AGCATACACACAATCCTTGT+TGG + chr1.2:61933589-61933608 MS.gene005060:CDS 40.0%
AGTGCTTGTGTGTGTATCAA+GGG + chr1.2:61934853-61934872 MS.gene005060:CDS 40.0%
AGTTAAGGATAGATGCATGC+TGG + chr1.2:61936898-61936917 MS.gene005060:CDS 40.0%
ATTACCAAACTCTCAGGGTT+TGG + chr1.2:61936508-61936527 MS.gene005060:CDS 40.0%
ATTCACATGCCTGAAATGGA+TGG + chr1.2:61936791-61936810 MS.gene005060:CDS 40.0%
CAAAAGGATCCTTCCAACAT+GGG - chr1.2:61935889-61935908 None:intergenic 40.0%
CAACGAAACAACAGATTGGT+AGG + chr1.2:61935147-61935166 MS.gene005060:CDS 40.0%
CACAACAGTGAATCTAAGGA+GGG + chr1.2:61935179-61935198 MS.gene005060:CDS 40.0%
CATCTGCTAGCAAAATCTTG+AGG - chr1.2:61936631-61936650 None:intergenic 40.0%
CCCTCTCCTTCTTAGAATTA+AGG + chr1.2:61933879-61933898 MS.gene005060:CDS 40.0%
CCTTCTACATTCCATGTTGA+GGG + chr1.2:61934812-61934831 MS.gene005060:CDS 40.0%
CTAGTAACAGCAGTTTCATC+AGG + chr1.2:61936704-61936723 MS.gene005060:CDS 40.0%
GCAACAGATCAGTTATTGGT+CGG - chr1.2:61935575-61935594 None:intergenic 40.0%
GCAACTCCTAGTTTAGATTC+AGG - chr1.2:61934246-61934265 None:intergenic 40.0%
GCAAGGATCAGGAAATTCAA+TGG + chr1.2:61936827-61936846 MS.gene005060:CDS 40.0%
GCATGTTAGGATGCTTACTA+GGG + chr1.2:61933984-61934003 MS.gene005060:CDS 40.0%
GCTATGATCAAAGGCCAATT+AGG - chr1.2:61936854-61936873 None:intergenic 40.0%
GGAAATTCAATGGCCCTAAT+TGG + chr1.2:61936837-61936856 MS.gene005060:CDS 40.0%
GGATGCTTACTAGGGAAATT+AGG + chr1.2:61933992-61934011 MS.gene005060:CDS 40.0%
GGCATCATTAGAGAAACATG+AGG + chr1.2:61936003-61936022 MS.gene005060:intron 40.0%
GGTGACACAATGTTGAAAGT+CGG + chr1.2:61934705-61934724 MS.gene005060:CDS 40.0%
GTTGACCTGCATCTTCATTT+GGG + chr1.2:61935373-61935392 MS.gene005060:intron 40.0%
TCAAAAGGATCCTTCCAACA+TGG - chr1.2:61935890-61935909 None:intergenic 40.0%
TCACAACAGTGAATCTAAGG+AGG + chr1.2:61935178-61935197 MS.gene005060:CDS 40.0%
TCCTTATCTCCAACACATCA+GGG - chr1.2:61934197-61934216 None:intergenic 40.0%
TCCTTCTACATTCCATGTTG+AGG + chr1.2:61934811-61934830 MS.gene005060:CDS 40.0%
TGTTGACCTGCATCTTCATT+TGG + chr1.2:61935372-61935391 MS.gene005060:intron 40.0%
TTCCTTATCTCCAACACATC+AGG - chr1.2:61934198-61934217 None:intergenic 40.0%
TTGAGGCCTGAATCTAAACT+AGG + chr1.2:61934237-61934256 MS.gene005060:CDS 40.0%
TTGATCCCAAATGAAGATGC+AGG - chr1.2:61935381-61935400 None:intergenic 40.0%
TTGTGTGTGTATCAAGGGTT+TGG + chr1.2:61934858-61934877 MS.gene005060:CDS 40.0%
TTTCTTGAGGCAGAATGTGA+TGG + chr1.2:61933912-61933931 MS.gene005060:CDS 40.0%
TTTGGATGCCGAATGATGAT+AGG + chr1.2:61934094-61934113 MS.gene005060:CDS 40.0%
! AGGCAGAATGTGATGGAATT+GGG + chr1.2:61933919-61933938 MS.gene005060:CDS 40.0%
! CCCTCACCTTAATTCTAAGA+AGG - chr1.2:61933888-61933907 None:intergenic 40.0%
! CGGGTTTTTGTTACAAGTGA+GGG + chr1.2:61935008-61935027 MS.gene005060:CDS 40.0%
! CTAAAGGGGTTAGGATTTTG+AGG + chr1.2:61934220-61934239 MS.gene005060:CDS 40.0%
! GTTTTTGTTACAAGTGAGGG+TGG + chr1.2:61935011-61935030 MS.gene005060:CDS 40.0%
! TATTTTTCCCCCATGTTGGA+AGG + chr1.2:61935877-61935896 MS.gene005060:intron 40.0%
! TCGGGTTTTTGTTACAAGTG+AGG + chr1.2:61935007-61935026 MS.gene005060:CDS 40.0%
! TTTGAGACTGTTGCAATGCT+CGG - chr1.2:61934545-61934564 None:intergenic 40.0%
!! CTTGTTATTCCCTTGCCTAT+TGG + chr1.2:61936356-61936375 MS.gene005060:intron 40.0%
!! GATAGCGGATGAACTTAGAT+CGG + chr1.2:61936964-61936983 MS.gene005060:CDS 40.0%
!! TGTTGAGCTTTCTAAGGCTT+TGG + chr1.2:61934053-61934072 MS.gene005060:CDS 40.0%
!!! AAGCTATTTTTCCCCCATGT+TGG + chr1.2:61935873-61935892 MS.gene005060:intron 40.0%
!!! AAGTTTTAGGCGATGAAGCA+AGG + chr1.2:61934916-61934935 MS.gene005060:CDS 40.0%
!!! AATGGATGGTTTTGAAGTGG+CGG + chr1.2:61936805-61936824 MS.gene005060:CDS 40.0%
!!! ACCGAGGGAATCTTGTTTTT+CGG + chr1.2:61934988-61935007 MS.gene005060:CDS 40.0%
!!! AGTTTTGGTTCTTCCAGGTT+CGG + chr1.2:61934389-61934408 MS.gene005060:CDS 40.0%
!!! CACTGACTTTTTGACCAACA+AGG - chr1.2:61933606-61933625 None:intergenic 40.0%
AAACTAGGAGTTGCGAGTAG+TGG + chr1.2:61934252-61934271 MS.gene005060:CDS 45.0%
AAAGGATCCTTCCAACATGG+GGG - chr1.2:61935887-61935906 None:intergenic 45.0%
AACACATCAGGGTCAATCAC+AGG - chr1.2:61934186-61934205 None:intergenic 45.0%
ACCCTGATGTGTTGGAGATA+AGG + chr1.2:61934193-61934212 MS.gene005060:CDS 45.0%
ACCTCAACTATCTCCATCTC+TGG - chr1.2:61934435-61934454 None:intergenic 45.0%
AGAGATGGAGATAGTTGAGG+TGG + chr1.2:61934434-61934453 MS.gene005060:CDS 45.0%
AGGAAGCTAGTTGGCATGTT+AGG + chr1.2:61933971-61933990 MS.gene005060:CDS 45.0%
AGGCCAATGCATTCAGTTGT+GGG + chr1.2:61934630-61934649 MS.gene005060:CDS 45.0%
ATCCCCACAACTGAATGCAT+TGG - chr1.2:61934636-61934655 None:intergenic 45.0%
ATGGAATTGGGGCAAGAAGT+TGG + chr1.2:61933931-61933950 MS.gene005060:CDS 45.0%
CAAGCACTTAGCAATGCAAG+CGG - chr1.2:61934841-61934860 None:intergenic 45.0%
CCAGGATATGCTCCTAAAAG+AGG - chr1.2:61935953-61935972 None:intergenic 45.0%
CCCGAAAAACAAGATTCCCT+CGG - chr1.2:61934992-61935011 None:intergenic 45.0%
CCCTCAACATGGAATGTAGA+AGG - chr1.2:61934815-61934834 None:intergenic 45.0%
CCTCATCATCACAGTTACAG+TGG - chr1.2:61933558-61933577 None:intergenic 45.0%
CGAAACAACAGATTGGTAGG+AGG + chr1.2:61935150-61935169 MS.gene005060:CDS 45.0%
CTAAGAAGGAGAGGGATCAA+CGG - chr1.2:61933874-61933893 None:intergenic 45.0%
CTCATATGAGGTGCATGTGA+GGG + chr1.2:61935548-61935567 MS.gene005060:intron 45.0%
CTGGAATGAATGGTGTGATC+CGG + chr1.2:61936921-61936940 MS.gene005060:CDS 45.0%
GCCTGCAACAGATCAGTTAT+TGG - chr1.2:61935579-61935598 None:intergenic 45.0%
GCTATCTGATGAAGGAGGAT+TGG - chr1.2:61936950-61936969 None:intergenic 45.0%
GCTTGAGCTTCAATATGTGC+CGG + chr1.2:61935201-61935220 MS.gene005060:CDS 45.0%
GGCATGTTAGGATGCTTACT+AGG + chr1.2:61933983-61934002 MS.gene005060:CDS 45.0%
GTCTCAAAAGAACGCGATGA+TGG + chr1.2:61934557-61934576 MS.gene005060:CDS 45.0%
GTTCATCCGCTATCTGATGA+AGG - chr1.2:61936958-61936977 None:intergenic 45.0%
TACAAGTGAGGGTGGTGATA+AGG + chr1.2:61935019-61935038 MS.gene005060:CDS 45.0%
TCCAGAGATGGAGATAGTTG+AGG + chr1.2:61934431-61934450 MS.gene005060:CDS 45.0%
TCCTTGACCTTCTAGTCAGA+TGG + chr1.2:61935642-61935661 MS.gene005060:intron 45.0%
TCTCATATGAGGTGCATGTG+AGG + chr1.2:61935547-61935566 MS.gene005060:intron 45.0%
TGACAAAGAAGCTACTCGAG+AGG + chr1.2:61936672-61936691 MS.gene005060:CDS 45.0%
TGGAATTGGGGCAAGAAGTT+GGG + chr1.2:61933932-61933951 MS.gene005060:CDS 45.0%
TGTGATTGACCCTGATGTGT+TGG + chr1.2:61934185-61934204 MS.gene005060:CDS 45.0%
TTCCAAACAGTGATGAGCCA+TGG + chr1.2:61934600-61934619 MS.gene005060:CDS 45.0%
! AACTATCTCCATCTCTGGAG+CGG - chr1.2:61934430-61934449 None:intergenic 45.0%
! AGGTGAGGGAGATTTTCTTG+AGG + chr1.2:61933899-61933918 MS.gene005060:CDS 45.0%
! CCACTGTAACTGTGATGATG+AGG + chr1.2:61933555-61933574 MS.gene005060:CDS 45.0%
! GAGGCAGAATGTGATGGAAT+TGG + chr1.2:61933918-61933937 MS.gene005060:CDS 45.0%
! GGCAGAATGTGATGGAATTG+GGG + chr1.2:61933920-61933939 MS.gene005060:CDS 45.0%
! TTTGGAATGACTTCCTAGCC+TGG - chr1.2:61934587-61934606 None:intergenic 45.0%
!! CCTCTTTTAGGAGCATATCC+TGG + chr1.2:61935950-61935969 MS.gene005060:intron 45.0%
!! GATGAAGGAGGATTGGTTTC+CGG - chr1.2:61936943-61936962 None:intergenic 45.0%
!!! AGCTTGTGCTTTTTGGGGTT+AGG + chr1.2:61936170-61936189 MS.gene005060:intron 45.0%
!!! ATCTGCTTTTTCGACTAGCC+AGG - chr1.2:61935971-61935990 None:intergenic 45.0%
!!! CCGAGGGAATCTTGTTTTTC+GGG + chr1.2:61934989-61935008 MS.gene005060:CDS 45.0%
!!! TGTGCTTTTTGGGGTTAGGT+GGG + chr1.2:61936174-61936193 MS.gene005060:intron 45.0%
!!! TTGTGCTTTTTGGGGTTAGG+TGG + chr1.2:61936173-61936192 MS.gene005060:intron 45.0%
AAAGAAGCTACTCGAGAGGC+TGG + chr1.2:61936676-61936695 MS.gene005060:CDS 50.0%
AAGAAGCTACTCGAGAGGCT+GGG + chr1.2:61936677-61936696 MS.gene005060:CDS 50.0%
AAGAGTCTCGACACAACGGT+TGG - chr1.2:61935676-61935695 None:intergenic 50.0%
AAGGATAGATGCATGCTGGC+TGG + chr1.2:61936902-61936921 MS.gene005060:CDS 50.0%
ACACAGGGCCTATTTCGTAC+AGG - chr1.2:61936393-61936412 None:intergenic 50.0%
ACTCCTGGACCAATAGGCAA+GGG - chr1.2:61936368-61936387 None:intergenic 50.0%
AGGAGTGGCCTGTACGAAAT+AGG + chr1.2:61936382-61936401 MS.gene005060:intron 50.0%
ATTCCCTTGCCTATTGGTCC+AGG + chr1.2:61936362-61936381 MS.gene005060:intron 50.0%
CATCCGCTATCTGATGAAGG+AGG - chr1.2:61936955-61936974 None:intergenic 50.0%
CTTCAATATGTGCCGGAGGT+TGG + chr1.2:61935208-61935227 MS.gene005060:CDS 50.0%
GAATTTCAAAGGAGGGACGC+CGG + chr1.2:61934338-61934357 MS.gene005060:CDS 50.0%
GAGGCCAATGCATTCAGTTG+TGG + chr1.2:61934629-61934648 MS.gene005060:CDS 50.0%
GATGGAGATAGTTGAGGTGG+TGG + chr1.2:61934437-61934456 MS.gene005060:CDS 50.0%
GCCATCTGACTAGAAGGTCA+AGG - chr1.2:61935646-61935665 None:intergenic 50.0%
GGCCAATGCATTCAGTTGTG+GGG + chr1.2:61934631-61934650 MS.gene005060:CDS 50.0%
GTCCATGGCTCATCACTGTT+TGG - chr1.2:61934605-61934624 None:intergenic 50.0%
GTGGTTGCCATCTGACTAGA+AGG - chr1.2:61935652-61935671 None:intergenic 50.0%
TATTTCGTACAGGCCACTCC+TGG - chr1.2:61936383-61936402 None:intergenic 50.0%
TCATCAGGGTTTGAGTGCCT+GGG + chr1.2:61936719-61936738 MS.gene005060:CDS 50.0%
TCTTCCAGGTTCGGACTCTA+GGG + chr1.2:61934398-61934417 MS.gene005060:CDS 50.0%
TGAGCTTCAATATGTGCCGG+AGG + chr1.2:61935204-61935223 MS.gene005060:CDS 50.0%
TGCACCAAACCCTGAGAGTT+TGG - chr1.2:61936515-61936534 None:intergenic 50.0%
TGCTCTTGTGTCGTGTGCAA+CGG + chr1.2:61933834-61933853 MS.gene005060:CDS 50.0%
TTCATCAGGGTTTGAGTGCC+TGG + chr1.2:61936718-61936737 MS.gene005060:CDS 50.0%
TTCTTCCAGGTTCGGACTCT+AGG + chr1.2:61934397-61934416 MS.gene005060:CDS 50.0%
! TGCATGCTGGCTGGAATGAA+TGG + chr1.2:61936911-61936930 MS.gene005060:CDS 50.0%
!! AACTCTCAGGGTTTGGTGCA+AGG + chr1.2:61936515-61936534 MS.gene005060:CDS 50.0%
!!! ATGGTTTTGAAGTGGCGGCA+AGG + chr1.2:61936810-61936829 MS.gene005060:CDS 50.0%
AGAAGGAGAGGGATCAACGG+CGG - chr1.2:61933871-61933890 None:intergenic 55.0%
CAAACCCTAGAGTCCGAACC+TGG - chr1.2:61934405-61934424 None:intergenic 55.0%
CACACGACACAAGAGCAGTG+AGG - chr1.2:61933831-61933850 None:intergenic 55.0%
CACCTACCTGTACCAACCTC+CGG - chr1.2:61935223-61935242 None:intergenic 55.0%
CACTCCTGGACCAATAGGCA+AGG - chr1.2:61936369-61936388 None:intergenic 55.0%
CAGAGCCACTTATAGCACCC+AGG - chr1.2:61936739-61936758 None:intergenic 55.0%
CTAGGAGTTGCGAGTAGTGG+CGG + chr1.2:61934255-61934274 MS.gene005060:CDS 55.0%
CTTGCCTATTGGTCCAGGAG+TGG + chr1.2:61936367-61936386 MS.gene005060:intron 55.0%
GAATGACTTCCTAGCCTGGC+TGG - chr1.2:61934583-61934602 None:intergenic 55.0%
GAGAACCGACGCGTGAGAAA+GGG - chr1.2:61934481-61934500 None:intergenic 55.0%
TCTCGACACAACGGTTGGAG+TGG - chr1.2:61935671-61935690 None:intergenic 55.0%
TGTTGAACACGCGTGACCGA+GGG + chr1.2:61934973-61934992 MS.gene005060:CDS 55.0%
TTGAAGTGGCGGCAAGGATC+AGG + chr1.2:61936816-61936835 MS.gene005060:CDS 55.0%
TTGTTGAACACGCGTGACCG+AGG + chr1.2:61934972-61934991 MS.gene005060:CDS 55.0%
! AGTTGAGGTGGTGGCTGATC+AGG + chr1.2:61934446-61934465 MS.gene005060:CDS 55.0%
! CAGTGATGAGCCATGGACTG+AGG + chr1.2:61934607-61934626 MS.gene005060:CDS 55.0%
! CTGGGTGCTATAAGTGGCTC+TGG + chr1.2:61936737-61936756 MS.gene005060:CDS 55.0%
! GAGTGCCTGGGTGCTATAAG+TGG + chr1.2:61936731-61936750 MS.gene005060:CDS 55.0%
! TGATGAGCCATGGACTGAGG+AGG + chr1.2:61934610-61934629 MS.gene005060:CDS 55.0%
!! CAGGTTCGGACTCTAGGGTT+TGG + chr1.2:61934403-61934422 MS.gene005060:CDS 55.0%
!! GGTGCTATAAGTGGCTCTGG+TGG + chr1.2:61936740-61936759 MS.gene005060:CDS 55.0%
!! TATGTGCCGGAGGTTGGTAC+AGG + chr1.2:61935214-61935233 MS.gene005060:CDS 55.0%
AGTTGCGAGTAGTGGCGGTG+GGG + chr1.2:61934260-61934279 MS.gene005060:CDS 60.0%
CAGGCCACTCCTGGACCAAT+AGG - chr1.2:61936374-61936393 None:intergenic 60.0%
CGACGCGTGAGAAAGGGCTA+CGG - chr1.2:61934475-61934494 None:intergenic 60.0%
CGAGAACCGACGCGTGAGAA+AGG - chr1.2:61934482-61934501 None:intergenic 60.0%
CGTAGCCCTTTCTCACGCGT+CGG + chr1.2:61934473-61934492 MS.gene005060:CDS 60.0%
GAGTTGCGAGTAGTGGCGGT+GGG + chr1.2:61934259-61934278 MS.gene005060:CDS 60.0%
GCAAGCGGCTTCCCTCAACA+TGG - chr1.2:61934826-61934845 None:intergenic 60.0%
GGTTTGGACCGCTCCAGAGA+TGG + chr1.2:61934419-61934438 MS.gene005060:CDS 60.0%
! GCATTGGCCTCCTCAGTCCA+TGG - chr1.2:61934620-61934639 None:intergenic 60.0%
! TGAGGAGTTGGGTGCTGTCG+CGG + chr1.2:61934287-61934306 MS.gene005060:CDS 60.0%
! TGAGGTGGTGGCTGATCAGG+TGG + chr1.2:61934449-61934468 MS.gene005060:CDS 60.0%
!! GGTGGGGGTACTGAGGAGTT+GGG + chr1.2:61934276-61934295 MS.gene005060:CDS 60.0%
!! TGCCGGAGGTTGGTACAGGT+AGG + chr1.2:61935218-61935237 MS.gene005060:intron 60.0%
CAAAGGAGGGACGCCGGAGT+TGG + chr1.2:61934344-61934363 MS.gene005060:CDS 65.0%
GGAGTTGCGAGTAGTGGCGG+TGG + chr1.2:61934258-61934277 MS.gene005060:CDS 65.0%
GTTGCGAGTAGTGGCGGTGG+GGG + chr1.2:61934261-61934280 MS.gene005060:CDS 65.0%
! CGATGATGGCCAGCCAGGCT+AGG + chr1.2:61934571-61934590 MS.gene005060:CDS 65.0%
! GAACGCGATGATGGCCAGCC+AGG + chr1.2:61934566-61934585 MS.gene005060:CDS 65.0%
!! CGGTGGGGGTACTGAGGAGT+TGG + chr1.2:61934275-61934294 MS.gene005060:CDS 65.0%
!! TAGTGGCGGTGGGGGTACTG+AGG + chr1.2:61934269-61934288 MS.gene005060:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 61933551 61937001 61933551 ID=MS.gene005060
chr1.2 mRNA 61933551 61937001 61933551 ID=MS.gene005060.t1;Parent=MS.gene005060
chr1.2 exon 61933551 61933618 61933551 ID=MS.gene005060.t1.exon1;Parent=MS.gene005060.t1
chr1.2 CDS 61933551 61933618 61933551 ID=cds.MS.gene005060.t1;Parent=MS.gene005060.t1
chr1.2 exon 61933783 61935235 61933783 ID=MS.gene005060.t1.exon2;Parent=MS.gene005060.t1
chr1.2 CDS 61933783 61935235 61933783 ID=cds.MS.gene005060.t1;Parent=MS.gene005060.t1
chr1.2 exon 61936485 61937001 61936485 ID=MS.gene005060.t1.exon3;Parent=MS.gene005060.t1
chr1.2 CDS 61936485 61937001 61936485 ID=cds.MS.gene005060.t1;Parent=MS.gene005060.t1
Gene Sequence

>MS.gene005060

ATGACCACTGTAACTGTGATGATGAGGAAGCACTTTTCAGCATACACACAATCCTTGTTGGTCAAAAAGTCAGTGATTTTTTCATTGCAATTGCATATTTTTCAATACCAATTGAGCTTCTTTACTTTGTTAGTCGTTCAAATGTTCCTTTCAAATTGTTGTTTCTTCAATTCATAGCCTTCATAGTTCTATGTGGTATGACTCATTTACTCAATGCTTATTCTTATCATAGTCATCCTTCTTTTCAACTCTTGCTTTCCCTTACTGTTGCTAAATTCCTCACTGCTCTTGTGTCGTGTGCAACGGCATTGACACTTCCGCCGTTGATCCCTCTCCTTCTTAGAATTAAGGTGAGGGAGATTTTCTTGAGGCAGAATGTGATGGAATTGGGGCAAGAAGTTGGGATTATGAAGAAACAAAAGGAAGCTAGTTGGCATGTTAGGATGCTTACTAGGGAAATTAGGAAGTCTCTTGATAAACATACAATTTTGTATACTACTCTTGTTGAGCTTTCTAAGGCTTTGGATTTGCATAATTGTGCTGTTTGGATGCCGAATGATGATAGGAGAGAGATGTATTTGACTCATGAATTGAAATCGGATTCGGGTAAGAGTTTTCATCATAATTCTATTCCTGTGATTGACCCTGATGTGTTGGAGATAAGGAAAACTAAAGGGGTTAGGATTTTGAGGCCTGAATCTAAACTAGGAGTTGCGAGTAGTGGCGGTGGGGGTACTGAGGAGTTGGGTGCTGTCGCGGCTATTCGTATGCCGATGCTTCATGTTTCGAATTTCAAAGGAGGGACGCCGGAGTTGGTTGATACATGTTATGCTATACTAGTTTTGGTTCTTCCAGGTTCGGACTCTAGGGTTTGGACCGCTCCAGAGATGGAGATAGTTGAGGTGGTGGCTGATCAGGTGGCCGTAGCCCTTTCTCACGCGTCGGTTCTCGAAGAGTCTTATTTAATGAGAGAGAAACTCGAAGAGCAAAACCGAGCATTGCAACAGTCTCAAAAGAACGCGATGATGGCCAGCCAGGCTAGGAAGTCATTCCAAACAGTGATGAGCCATGGACTGAGGAGGCCAATGCATTCAGTTGTGGGGATGCTTTCATTGTTTCAAGAGGGTAATATGAGGACTGAACAAAAGATTATTGGTGACACAATGTTGAAAGTCGGCAATGTGCTCTCGAGTTTAGTAAACGATGTAATGGATATCTCTGAAAATAAAAAGGGTGGCCTTCAGTTAGAGATGAAACCTTTCCTTCTACATTCCATGTTGAGGGAAGCCGCTTGCATTGCTAAGTGCTTGTGTGTGTATCAAGGGTTTGGTTTTCAAATTGATGTCGAAAAGTCTTTGCCTGAAAAAGTTTTAGGCGATGAAGCAAGGACTTTTCAAGTAATTATGCATATTATTGGCTATTTGTTGAACACGCGTGACCGAGGGAATCTTGTTTTTCGGGTTTTTGTTACAAGTGAGGGTGGTGATAAGGATGACAAAAAAATTGGTGTATGGAGATCGAGCAGCCCAGTTGAGTATGTAGACATAAAATTTGATTTTCAGATTACTGGTAGTTCTCAGTCAGATGAACCGATTTCAACGAAACAACAGATTGGTAGGAGGCTGAATCACAACAGTGAATCTAAGGAGGGCTTGAGCTTCAATATGTGCCGGAGGTTGGTACAGGTAGGTGCATCACTGTCTATATTATATATGTGGATCATAATCTCCTTGATTGTCTATGTTATGTCTGAGTATCAAATATGTCATTTATTGTTCAAATTTAATGCTTAAAGAATGATGTTTGGTTTGACGAATTTAATGTTGACCTGCATCTTCATTTGGGATCAAAATGAATGCTAGCATGCTTGCTACTAAACTTTAGTGAAAGTTTTTGTTTCTTCAAACCATTCATTAAGGATGTAAAATTATGTGTGATCACTTATCTGTTCATTCGAAGTCACTGATTACTTATATTTAAAAAGTGCAATAACTGATCTCATATGAGGTGCATGTGAGGGTCCGACCAATAACTGATCTGTTGCAGGCAATCTTACCCTGCAATTGCAAGAAACTGATTCTACGAATCGGATCCTTGACCTTCTAGTCAGATGGCAACCACTCCAACCGTTGTGTCGAGACTCTTTTTTTCACTGATAACTTATATTTGACAATTTTTCGTAGATTTCTCTCTCCATACTAATTTCAGCTAAGAGCTGACAAGCCTCCTCTGTTATAACCTAAATCTTTCAACATAAGAAATAAAATAAAAAAATTAATAAGAACATAATTGTAATCCTTTTATCTTAATAGTATCACGGAGAAGCTATTTTTCCCCCATGTTGGAAGGATCCTTTTGAGTTTACATCACACAAGCATGCATTTGCATAATTTTGTTTTCCTCTTTTAGGAGCATATCCTGGCTAGTCGAAAAAGCAGATTCTGACATGACAGGCATCATTAGAGAAACATGAGGATTGTAATAACACAGAAACTGCATGATTGTGGTGTCTTTTCTTGAATTGTAAGGTCATTCAGTTAGTTTTAGAATTCCAATATGAACAAGACTACAAAAAATCTCACTTCCTTTTCATCAATGGAAATAGGTGAACAATAAGAAAGCTTGTGCTTTTTGGGGTTAGGTGGGTGCATTATTTTATTATATAGAAAAATAATAGTAAAGTAGCATCTTTTGTGCCTTAAAATCTGGTAAAGTAGGACCTTACTTCTTTATACTTATAATAGTATCCAATTATTTTATTAAAGCACTATTATGATTTCTCTCTCTTTTTGCCACAAATCAGAACTTGTTATTCCCTTGCCTATTGGTCCAGGAGTGGCCTGTACGAAATAGGCCCTGTGTAATTATTTAATTAATCATGGCATGCTTGTTTTTGTAATTCTGACTTTATTTACATTGTGGAATCTTATGCAGATAATGCAAGGTAATATATGGATATTACCAAACTCTCAGGGTTTGGTGCAAGGCGTGTCACTTCTTCTAAAGTTTCAGATAGGACCATCGCTTGAAAGATACATGTTTGCACCTAAAGATTATTCAAATTCACAGTTTGGAGGCCTCAAGATTTTGCTAGCAGATGATGATAGTTTAAATAGGATCGTGACAAAGAAGCTACTCGAGAGGCTGGGCTGTCTAGTAACAGCAGTTTCATCAGGGTTTGAGTGCCTGGGTGCTATAAGTGGCTCTGGTGGTAACTCATTCAAAATTATCATGTTAGACATTCACATGCCTGAAATGGATGGTTTTGAAGTGGCGGCAAGGATCAGGAAATTCAATGGCCCTAATTGGCCTTTGATCATAGCTCTTATAGCTAATGCAGAAGAGCAAGTTAAGGATAGATGCATGCTGGCTGGAATGAATGGTGTGATCCGGAAACCAATCCTCCTTCATCAGATAGCGGATGAACTTAGATCGGTCCTACTTCGTGCAG
Protein sequence

>MS.gene005060.t1

MTTVTVMMRKHFSAYTQSLLVKNHPSFQLLLSLTVAKFLTALVSCATALTLPPLIPLLLRIKVREIFLRQNVMELGQEVGIMKKQKEASWHVRMLTREIRKSLDKHTILYTTLVELSKALDLHNCAVWMPNDDRREMYLTHELKSDSGKSFHHNSIPVIDPDVLEIRKTKGVRILRPESKLGVASSGGGGTEELGAVAAIRMPMLHVSNFKGGTPELVDTCYAILVLVLPGSDSRVWTAPEMEIVEVVADQVAVALSHASVLEESYLMREKLEEQNRALQQSQKNAMMASQARKSFQTVMSHGLRRPMHSVVGMLSLFQEGNMRTEQKIIGDTMLKVGNVLSSLVNDVMDISENKKGGLQLEMKPFLLHSMLREAACIAKCLCVYQGFGFQIDVEKSLPEKVLGDEARTFQVIMHIIGYLLNTRDRGNLVFRVFVTSEGGDKDDKKIGVWRSSSPVEYVDIKFDFQITGSSQSDEPISTKQQIGRRLNHNSESKEGLSFNMCRRLVQIMQGNIWILPNSQGLVQGVSLLLKFQIGPSLERYMFAPKDYSNSQFGGLKILLADDDSLNRIVTKKLLERLGCLVTAVSSGFECLGAISGSGGNSFKIIMLDIHMPEMDGFEVAARIRKFNGPNWPLIIALIANAEEQVKDRCMLAGMNGVIRKPILLHQIADELRSVLLRA