Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005602.t1 | XP_003591541.1 | 96.5 | 170 | 3 | 1 | 1 | 170 | 1 | 167 | 4.50E-82 | 313.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005602.t1 | Q8L4N1 | 31.5 | 162 | 93 | 4 | 19 | 166 | 49 | 206 | 4.6e-11 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005602.t1 | G7IF74 | 96.5 | 170 | 3 | 1 | 1 | 170 | 1 | 167 | 3.2e-82 | 313.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene005602 | MS.gene005525 | PPI |
| MS.gene20848 | MS.gene005602 | PPI |
| MS.gene005602 | MS.gene037687 | PPI |
| MS.gene07276 | MS.gene005602 | PPI |
| MS.gene92557 | MS.gene005602 | PPI |
| MS.gene20798 | MS.gene005602 | PPI |
| MS.gene73234 | MS.gene005602 | PPI |
| MS.gene048106 | MS.gene005602 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005602.t1 | MTR_1g088640 | 96.471 | 170 | 3 | 1 | 1 | 170 | 1 | 167 | 1.47e-117 | 329 |
| MS.gene005602.t1 | MTR_1g088640 | 94.706 | 170 | 3 | 2 | 1 | 170 | 1 | 164 | 4.31e-113 | 318 |
| MS.gene005602.t1 | MTR_7g108830 | 68.590 | 156 | 46 | 2 | 16 | 170 | 9 | 162 | 2.98e-66 | 199 |
| MS.gene005602.t1 | MTR_8g058620 | 68.590 | 156 | 46 | 2 | 16 | 170 | 9 | 162 | 2.98e-66 | 199 |
| MS.gene005602.t1 | MTR_1g088620 | 100.000 | 69 | 0 | 0 | 102 | 170 | 218 | 286 | 4.71e-42 | 142 |
| MS.gene005602.t1 | MTR_1g087200 | 40.252 | 159 | 90 | 3 | 11 | 166 | 2 | 158 | 3.59e-34 | 118 |
| MS.gene005602.t1 | MTR_1g054765 | 40.462 | 173 | 79 | 5 | 18 | 170 | 6 | 174 | 5.15e-33 | 115 |
| MS.gene005602.t1 | MTR_1g083950 | 39.752 | 161 | 89 | 4 | 10 | 168 | 5 | 159 | 1.69e-32 | 114 |
| MS.gene005602.t1 | MTR_1g054750 | 37.805 | 164 | 90 | 3 | 18 | 170 | 25 | 187 | 1.36e-30 | 110 |
| MS.gene005602.t1 | MTR_5g065980 | 32.716 | 162 | 96 | 3 | 16 | 164 | 9 | 170 | 4.36e-22 | 87.8 |
| MS.gene005602.t1 | MTR_5g065980 | 32.298 | 161 | 96 | 3 | 16 | 164 | 9 | 168 | 1.18e-21 | 86.7 |
| MS.gene005602.t1 | MTR_2g089350 | 29.560 | 159 | 99 | 4 | 19 | 167 | 29 | 184 | 1.28e-15 | 72.0 |
| MS.gene005602.t1 | MTR_4g064933 | 32.258 | 155 | 92 | 4 | 19 | 165 | 9 | 158 | 1.68e-15 | 70.5 |
| MS.gene005602.t1 | MTR_6g006190 | 24.051 | 158 | 109 | 3 | 17 | 165 | 8 | 163 | 1.53e-12 | 62.8 |
| MS.gene005602.t1 | MTR_5g077130 | 29.032 | 155 | 95 | 5 | 19 | 164 | 9 | 157 | 1.94e-12 | 62.4 |
| MS.gene005602.t1 | MTR_5g077130 | 28.846 | 156 | 95 | 5 | 19 | 164 | 9 | 158 | 2.21e-12 | 62.0 |
| MS.gene005602.t1 | MTR_2g034200 | 29.375 | 160 | 100 | 5 | 19 | 170 | 9 | 163 | 3.53e-12 | 61.6 |
| MS.gene005602.t1 | MTR_7g065770 | 29.936 | 157 | 91 | 6 | 19 | 164 | 9 | 157 | 1.41e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005602.t1 | AT2G47710 | 62.195 | 164 | 57 | 2 | 8 | 169 | 1 | 161 | 2.02e-70 | 210 |
| MS.gene005602.t1 | AT3G11930 | 40.449 | 178 | 87 | 6 | 7 | 167 | 20 | 195 | 7.28e-34 | 119 |
| MS.gene005602.t1 | AT3G11930 | 40.223 | 179 | 87 | 6 | 7 | 167 | 20 | 196 | 1.02e-33 | 118 |
| MS.gene005602.t1 | AT3G11930 | 40.000 | 180 | 87 | 6 | 7 | 167 | 20 | 197 | 1.30e-33 | 118 |
| MS.gene005602.t1 | AT1G09740 | 38.365 | 159 | 88 | 5 | 17 | 167 | 10 | 166 | 3.55e-28 | 103 |
| MS.gene005602.t1 | AT1G09740 | 38.365 | 159 | 88 | 5 | 17 | 167 | 10 | 166 | 3.55e-28 | 103 |
| MS.gene005602.t1 | AT3G11930 | 35.096 | 208 | 83 | 8 | 7 | 167 | 20 | 222 | 1.08e-27 | 103 |
| MS.gene005602.t1 | AT3G58450 | 37.349 | 166 | 83 | 5 | 17 | 167 | 32 | 191 | 4.77e-26 | 99.0 |
| MS.gene005602.t1 | AT3G62550 | 36.905 | 168 | 85 | 8 | 7 | 165 | 2 | 157 | 1.32e-25 | 96.7 |
| MS.gene005602.t1 | AT3G58450 | 36.145 | 166 | 78 | 6 | 17 | 167 | 32 | 184 | 4.74e-25 | 95.9 |
| MS.gene005602.t1 | AT5G49050 | 42.735 | 117 | 62 | 2 | 13 | 127 | 37 | 150 | 2.24e-24 | 93.2 |
| MS.gene005602.t1 | AT1G11360 | 32.571 | 175 | 94 | 6 | 10 | 169 | 33 | 198 | 9.02e-19 | 80.5 |
| MS.gene005602.t1 | AT1G11360 | 32.571 | 175 | 94 | 6 | 10 | 169 | 33 | 198 | 9.02e-19 | 80.5 |
| MS.gene005602.t1 | AT1G11360 | 32.571 | 175 | 94 | 6 | 10 | 169 | 33 | 198 | 9.02e-19 | 80.5 |
| MS.gene005602.t1 | AT1G11360 | 32.571 | 175 | 94 | 6 | 10 | 169 | 33 | 198 | 9.02e-19 | 80.5 |
| MS.gene005602.t1 | AT1G68300 | 32.278 | 158 | 100 | 4 | 11 | 164 | 3 | 157 | 2.68e-18 | 77.4 |
| MS.gene005602.t1 | AT5G14680 | 27.647 | 170 | 99 | 5 | 12 | 165 | 2 | 163 | 3.66e-16 | 72.4 |
| MS.gene005602.t1 | AT3G01520 | 28.676 | 136 | 95 | 2 | 30 | 165 | 30 | 163 | 7.10e-15 | 68.9 |
| MS.gene005602.t1 | AT3G17020 | 28.477 | 151 | 102 | 2 | 19 | 164 | 11 | 160 | 8.33e-13 | 63.2 |
| MS.gene005602.t1 | AT3G53990 | 25.325 | 154 | 102 | 4 | 19 | 164 | 9 | 157 | 2.76e-12 | 61.6 |
| MS.gene005602.t1 | AT2G21620 | 29.932 | 147 | 80 | 4 | 17 | 163 | 42 | 165 | 2.55e-11 | 59.7 |
| MS.gene005602.t1 | AT2G21620 | 30.612 | 147 | 85 | 4 | 17 | 163 | 42 | 171 | 2.61e-11 | 59.7 |
Find 0 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAATTAAAAAGCAAA+AGG | + | 8681:118365-118384 | None:intergenic | 10.0% |
| !! | ATAAAATTATGAAAAGAATT+AGG | + | 8681:117374-117393 | None:intergenic | 10.0% |
| !! | TAAAATTATGAAAAGAATTA+GGG | + | 8681:117373-117392 | None:intergenic | 10.0% |
| !!! | ATGATATTATTTTAGATAAT+AGG | - | 8681:117486-117505 | MS.gene005602:intron | 10.0% |
| !! | AAAAATGTTAGAATTAAATG+CGG | + | 8681:118148-118167 | None:intergenic | 15.0% |
| !!! | ACTTTTCATTTTATTTATAC+TGG | - | 8681:118386-118405 | MS.gene005602:intron | 15.0% |
| !!! | ATTACATTTATTCATGTTTT+TGG | + | 8681:118215-118234 | None:intergenic | 15.0% |
| !!! | CTTTTCATAATTTTATTTGA+TGG | - | 8681:117378-117397 | MS.gene005602:CDS | 15.0% |
| !!! | CTTTTCATTTTATTTATACT+GGG | - | 8681:118387-118406 | MS.gene005602:intron | 15.0% |
| !!! | TTTTCATTTTATTTATACTG+GGG | - | 8681:118388-118407 | MS.gene005602:intron | 15.0% |
| !! | AAAATTAAAAAGCAAAAGGA+AGG | + | 8681:118361-118380 | None:intergenic | 20.0% |
| !! | AACAAAAATAGAAACACATA+CGG | + | 8681:117188-117207 | None:intergenic | 20.0% |
| !! | TTAAGTATTTATGTCATATG+TGG | - | 8681:117872-117891 | MS.gene005602:intron | 20.0% |
| !!! | AAAATTTTAAACAACAGTCA+AGG | - | 8681:117224-117243 | MS.gene005602:intron | 20.0% |
| !!! | TTTCATTTTATTTATACTGG+GGG | - | 8681:118389-118408 | MS.gene005602:intron | 20.0% |
| ! | AATTTAAGTGGAAATACAAC+AGG | + | 8681:117670-117689 | None:intergenic | 25.0% |
| ! | ATGAAATTGTGAATTATACC+AGG | + | 8681:116999-117018 | None:intergenic | 25.0% |
| !!! | GCTTTTTTTTGTTGATGTTT+TGG | - | 8681:117031-117050 | MS.gene005602:intron | 25.0% |
| !!! | TGAATTTTCTCTGGTTTATA+CGG | - | 8681:117402-117421 | MS.gene005602:CDS | 25.0% |
| !!! | TTAATACACACAAGTTTTGT+TGG | + | 8681:117791-117810 | None:intergenic | 25.0% |
| !!! | TTTGTTGATGTTTTGGTAAT+TGG | - | 8681:117038-117057 | MS.gene005602:intron | 25.0% |
| !!! | TTTTTTGCTAGTTTGATGAA+AGG | - | 8681:117598-117617 | MS.gene005602:intron | 25.0% |
| AATTAAATGCGGCATATTCA+TGG | + | 8681:118137-118156 | None:intergenic | 30.0% | |
| CCTGTATTTATATGTTGTGT+GGG | - | 8681:117426-117445 | MS.gene005602:CDS | 30.0% | |
| TACAATTATCATCCTTCGTT+TGG | - | 8681:118065-118084 | MS.gene005602:intron | 30.0% | |
| TCAGGTAAATGATGTAATAG+TGG | - | 8681:117921-117940 | MS.gene005602:intron | 30.0% | |
| TCCTGTATTTATATGTTGTG+TGG | - | 8681:117425-117444 | MS.gene005602:CDS | 30.0% | |
| TTACCATCATCTTTGTATGT+CGG | - | 8681:117712-117731 | MS.gene005602:intron | 30.0% | |
| ! | TAATTTTGTGTTTGTGATCG+TGG | + | 8681:118187-118206 | None:intergenic | 30.0% |
| ! | TCTGATAACTGAATTTTCTC+AGG | - | 8681:117903-117922 | MS.gene005602:intron | 30.0% |
| !! | TTTGATGGATGAATTTTCTC+TGG | - | 8681:117393-117412 | MS.gene005602:CDS | 30.0% |
| !! | TTTTTCTACTTTCGAGTCTA+AGG | - | 8681:117827-117846 | MS.gene005602:intron | 30.0% |
| !!! | CTTTTCTGAAACTTGTGAAT+AGG | - | 8681:118106-118125 | MS.gene005602:intron | 30.0% |
| ACCAGTTGAAGCAATTTAAG+TGG | + | 8681:117682-117701 | None:intergenic | 35.0% | |
| AGGTTAAAGATTTCAGTGGA+TGG | + | 8681:118241-118260 | None:intergenic | 35.0% | |
| ATCTTTAACCTGCAGAAGAT+AGG | - | 8681:118250-118269 | MS.gene005602:CDS | 35.0% | |
| CAAAAGGAAGGTTTGATTGT+TGG | + | 8681:118349-118368 | None:intergenic | 35.0% | |
| CCCACACAACATATAAATAC+AGG | + | 8681:117429-117448 | None:intergenic | 35.0% | |
| CTCAAATCAGAATCCACAAT+TGG | + | 8681:117112-117131 | None:intergenic | 35.0% | |
| GGTAAATGATGTAATAGTGG+AGG | - | 8681:117924-117943 | MS.gene005602:intron | 35.0% | |
| TAACCGACATACAAAGATGA+TGG | + | 8681:117718-117737 | None:intergenic | 35.0% | |
| TATGGGGCTATAAAAAGGTA+TGG | - | 8681:118030-118049 | MS.gene005602:intron | 35.0% | |
| TCCACTTAAATTGCTTCAAC+TGG | - | 8681:117678-117697 | MS.gene005602:intron | 35.0% | |
| TCGATTGCAACTAACATTAC+TGG | + | 8681:116846-116865 | None:intergenic | 35.0% | |
| TTACATATCACTCCAAACGA+AGG | + | 8681:118080-118099 | None:intergenic | 35.0% | |
| TTTATATGTTGTGTGGGACT+TGG | - | 8681:117432-117451 | MS.gene005602:CDS | 35.0% | |
| ! | AAAAAGGTATGGCTTCATTG+TGG | - | 8681:118041-118060 | MS.gene005602:intron | 35.0% |
| ! | AAATGATGTAATAGTGGAGG+TGG | - | 8681:117927-117946 | MS.gene005602:intron | 35.0% |
| ! | TCGGTTAATTGTCATGCTTT+TGG | - | 8681:117731-117750 | MS.gene005602:intron | 35.0% |
| !! | AAAGTATCGTGCTTCAATCT+TGG | - | 8681:117990-118009 | MS.gene005602:intron | 35.0% |
| ACGGTTATGGGGCTATAAAA+AGG | - | 8681:118025-118044 | MS.gene005602:intron | 40.0% | |
| ATCTTGGTTGTAGGTAGTCA+CGG | - | 8681:118006-118025 | MS.gene005602:intron | 40.0% | |
| CTGCAGGTTAAAGATTTCAG+TGG | + | 8681:118245-118264 | None:intergenic | 40.0% | |
| TCGAGTGTAGTGAGTGAATT+AGG | + | 8681:118466-118485 | None:intergenic | 40.0% | |
| TTGTAGGTAGTCACGGTTAT+GGG | - | 8681:118013-118032 | MS.gene005602:intron | 40.0% | |
| TTGTGCTAATTAGTGTACGC+AGG | - | 8681:117070-117089 | MS.gene005602:intron | 40.0% | |
| ! | ACTCGACTCTTTTAATCTGC+AGG | - | 8681:118480-118499 | MS.gene005602:CDS | 40.0% |
| ! | CTCGACTCTTTTAATCTGCA+GGG | - | 8681:118481-118500 | MS.gene005602:CDS | 40.0% |
| ! | TTTTGTGTTTGTGATCGTGG+TGG | + | 8681:118184-118203 | None:intergenic | 40.0% |
| ACAGTCAAGGTGTGCGATTT+CGG | - | 8681:117237-117256 | MS.gene005602:intron | 45.0% | |
| AGTTCCATACCCACATCCTT+AGG | - | 8681:117764-117783 | MS.gene005602:intron | 45.0% | |
| CGTGCTTCAATCTTGGTTGT+AGG | - | 8681:117997-118016 | MS.gene005602:intron | 45.0% | |
| GGGACTTGGAGCATACTATA+TGG | - | 8681:117446-117465 | MS.gene005602:CDS | 45.0% | |
| GTTCCATACCCACATCCTTA+GGG | - | 8681:117765-117784 | MS.gene005602:intron | 45.0% | |
| GTTGTAGGTAGTCACGGTTA+TGG | - | 8681:118012-118031 | MS.gene005602:intron | 45.0% | |
| TAGCTGAATGCATCATGCTC+AGG | - | 8681:117523-117542 | MS.gene005602:intron | 45.0% | |
| TGTAGGTAGTCACGGTTATG+GGG | - | 8681:118014-118033 | MS.gene005602:intron | 45.0% | |
| TTTGTTGGCCCTAAGGATGT+GGG | + | 8681:117776-117795 | None:intergenic | 45.0% | |
| ! | GACTCTTTTAATCTGCAGGG+CGG | - | 8681:118484-118503 | MS.gene005602:CDS | 45.0% |
| ! | TGATGTAATAGTGGAGGTGG+TGG | - | 8681:117930-117949 | MS.gene005602:intron | 45.0% |
| ! | TGCTGAGGTTTTGCCAATTG+TGG | - | 8681:117096-117115 | MS.gene005602:intron | 45.0% |
| ! | TTAATCTGCAGGGCGGTTTT+AGG | - | 8681:118491-118510 | MS.gene005602:CDS | 45.0% |
| ! | TTTTGTTGGCCCTAAGGATG+TGG | + | 8681:117777-117796 | None:intergenic | 45.0% |
| !! | ACAGTGCATACGCTCTGAAA+TGG | - | 8681:116877-116896 | MS.gene005602:intron | 45.0% |
| !!! | CACAAGTTTTGTTGGCCCTA+AGG | + | 8681:117783-117802 | None:intergenic | 45.0% |
| GAATTATACCAGGACCAGCG+AGG | + | 8681:116989-117008 | None:intergenic | 50.0% | |
| GAGGCCAACAGAAGAAGTAG+CGG | + | 8681:116970-116989 | None:intergenic | 50.0% | |
| TACTGCTGCCTATCTTCTGC+AGG | + | 8681:118261-118280 | None:intergenic | 50.0% | |
| !! | GTAATAGTGGAGGTGGTGGA+AGG | - | 8681:117934-117953 | MS.gene005602:intron | 50.0% |
| CCAACAGAAGAAGTAGCGGC+GGG | + | 8681:116966-116985 | None:intergenic | 55.0% | |
| GCCAACAGAAGAAGTAGCGG+CGG | + | 8681:116967-116986 | None:intergenic | 55.0% | |
| TGGCCCTAAGGATGTGGGTA+TGG | + | 8681:117771-117790 | None:intergenic | 55.0% | |
| ! | ACTTCTTCTGTTGGCCTCGC+TGG | - | 8681:116972-116991 | MS.gene005602:intron | 55.0% |
| AGTGTACGCAGGAGCTGCTG+AGG | - | 8681:117081-117100 | MS.gene005602:intron | 60.0% | |
| CCCGCCGCTACTTCTTCTGT+TGG | - | 8681:116963-116982 | MS.gene005602:intron | 60.0% | |
| ! | TCTGTTGGCCTCGCTGGTCC+TGG | - | 8681:116978-116997 | MS.gene005602:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8681 | gene | 116801 | 118589 | 116801 | ID=MS.gene005602 |
| 8681 | mRNA | 116801 | 118589 | 116801 | ID=MS.gene005602.t1;Parent=MS.gene005602 |
| 8681 | exon | 118391 | 118589 | 118391 | ID=MS.gene005602.t1.exon1;Parent=MS.gene005602.t1 |
| 8681 | CDS | 118391 | 118589 | 118391 | ID=cds.MS.gene005602.t1;Parent=MS.gene005602.t1 |
| 8681 | exon | 118204 | 118307 | 118204 | ID=MS.gene005602.t1.exon2;Parent=MS.gene005602.t1 |
| 8681 | CDS | 118204 | 118307 | 118204 | ID=cds.MS.gene005602.t1;Parent=MS.gene005602.t1 |
| 8681 | exon | 117344 | 117465 | 117344 | ID=MS.gene005602.t1.exon3;Parent=MS.gene005602.t1 |
| 8681 | CDS | 117344 | 117465 | 117344 | ID=cds.MS.gene005602.t1;Parent=MS.gene005602.t1 |
| 8681 | exon | 116801 | 116888 | 116801 | ID=MS.gene005602.t1.exon4;Parent=MS.gene005602.t1 |
| 8681 | CDS | 116801 | 116888 | 116801 | ID=cds.MS.gene005602.t1;Parent=MS.gene005602.t1 |
| Gene Sequence |
| Protein sequence |
