AlfalfaGEDB | MS.gene005525

M. sativa cultivar XinJiangDaYe / MS.gene005525

KEGG: -
GO: -
PFAM: PF00582.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005525.t1	XP_003591417.1	98.7	158	2	0	1	158	1	158	1.30E-80	308.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005525.t1	Q8VYN9	31.9	163	94	4	7	158	49	205	8.7e-12	71.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005525.t1	G7IDR7	98.7	158	2	0	1	158	1	158	9.7e-81	308.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene005525	MS.gene063633	PPI
MS.gene005602	MS.gene005525	PPI
MS.gene005525	MS.gene037687	PPI
MS.gene005525	MS.gene050004	PPI
MS.gene07213	MS.gene005525	PPI
MS.gene048106	MS.gene005525	PPI
MS.gene26154	MS.gene005525	PPI
MS.gene06986	MS.gene005525	PPI
MS.gene20731	MS.gene005525	PPI
MS.gene009525	MS.gene005525	PPI
MS.gene005525	MS.gene010109	PPI
MS.gene017358	MS.gene005525	PPI
MS.gene92557	MS.gene005525	PPI
MS.gene22971	MS.gene005525	PPI
MS.gene73234	MS.gene005525	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005525.t1	MTR_1g087200	98.734	158	2	0	1	158	1	158	1.66e-114	321
MS.gene005525.t1	MTR_1g083950	52.597	154	67	3	6	158	9	157	7.02e-56	173
MS.gene005525.t1	MTR_1g088640	39.375	160	91	4	2	158	7	163	8.12e-33	114
MS.gene005525.t1	MTR_1g088640	39.873	158	90	4	2	158	7	160	1.64e-31	111
MS.gene005525.t1	MTR_1g054750	34.706	170	95	5	2	158	17	183	1.64e-30	109
MS.gene005525.t1	MTR_1g054765	36.471	170	87	5	7	158	4	170	1.67e-29	106
MS.gene005525.t1	MTR_7g108830	41.026	156	87	4	5	158	6	158	4.98e-27	99.8
MS.gene005525.t1	MTR_8g058620	41.026	156	87	4	5	158	6	158	4.98e-27	99.8
MS.gene005525.t1	MTR_5g065980	35.185	162	91	4	8	156	10	170	1.03e-26	99.4
MS.gene005525.t1	MTR_5g065980	35.185	162	89	4	8	156	10	168	1.24e-26	99.0
MS.gene005525.t1	MTR_5g077130	36.774	155	84	5	10	156	9	157	1.58e-21	85.5
MS.gene005525.t1	MTR_5g077130	36.538	156	84	6	10	156	9	158	2.43e-20	82.4
MS.gene005525.t1	MTR_2g089350	32.738	168	89	6	6	158	25	183	2.48e-20	84.0
MS.gene005525.t1	MTR_1g088620	58.462	65	25	1	94	158	220	282	4.37e-18	79.0
MS.gene005525.t1	MTR_4g019800	33.129	163	91	6	4	156	3	157	3.10e-17	74.3
MS.gene005525.t1	MTR_6g006190	27.168	173	100	4	1	157	1	163	9.42e-17	73.6
MS.gene005525.t1	MTR_2g034200	28.481	158	101	3	6	156	5	157	3.67e-16	71.6
MS.gene005525.t1	MTR_4g064933	35.404	161	88	5	6	157	5	158	1.12e-15	70.5
MS.gene005525.t1	MTR_5g065980	32.394	142	80	4	8	136	10	148	1.51e-15	70.9
MS.gene005525.t1	MTR_7g065770	29.091	165	95	4	4	156	3	157	2.60e-13	64.3
MS.gene005525.t1	MTR_6g006190	39.683	63	38	0	95	157	77	139	3.18e-12	60.8
MS.gene005525.t1	MTR_1717s0010	28.571	161	89	5	6	143	42	199	1.27e-11	60.8
MS.gene005525.t1	MTR_1527s0010	28.571	161	89	5	6	143	42	199	1.27e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005525.t1	AT3G62550	61.146	157	58	3	2	157	3	157	5.33e-64	193
MS.gene005525.t1	AT3G11930	36.970	165	89	4	6	158	33	194	3.38e-29	106
MS.gene005525.t1	AT3G11930	36.747	166	89	4	6	158	33	195	4.59e-29	105
MS.gene005525.t1	AT3G11930	36.527	167	89	4	6	158	33	196	6.35e-29	105
MS.gene005525.t1	AT2G47710	39.869	153	86	4	8	158	10	158	1.06e-27	101
MS.gene005525.t1	AT3G58450	35.802	162	90	5	8	158	32	190	3.17e-27	101
MS.gene005525.t1	AT1G09740	37.975	158	86	4	8	156	10	164	8.72e-27	99.4
MS.gene005525.t1	AT1G09740	37.975	158	86	4	8	156	10	164	8.72e-27	99.4
MS.gene005525.t1	AT3G58450	36.076	158	88	4	8	158	32	183	9.82e-27	100
MS.gene005525.t1	AT1G68300	35.714	154	90	3	6	156	10	157	4.20e-26	97.4
MS.gene005525.t1	AT1G11360	35.802	162	93	4	5	158	37	195	8.51e-24	93.6
MS.gene005525.t1	AT1G11360	35.802	162	93	4	5	158	37	195	8.51e-24	93.6
MS.gene005525.t1	AT1G11360	35.802	162	93	4	5	158	37	195	8.51e-24	93.6
MS.gene005525.t1	AT1G11360	35.802	162	93	4	5	158	37	195	8.51e-24	93.6
MS.gene005525.t1	AT3G11930	31.771	192	89	5	6	158	33	221	4.84e-23	91.3
MS.gene005525.t1	AT5G14680	26.506	166	110	3	1	157	1	163	2.71e-18	77.4
MS.gene005525.t1	AT3G01520	28.000	175	96	5	1	157	1	163	2.21e-17	75.1
MS.gene005525.t1	AT3G03270	30.357	168	89	4	4	156	3	157	1.17e-15	70.1
MS.gene005525.t1	AT3G53990	27.215	158	107	2	4	156	3	157	3.88e-15	68.9
MS.gene005525.t1	AT3G17020	29.518	166	98	4	2	156	3	160	8.91e-15	68.2

Find 27 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAATTACAACTGGGCATT+TGG	0.240443	1.1:-64627954	None:intergenic
CAAAGTATCAATTGTATCAT+TGG	0.275490	1.1:-64627502	None:intergenic
CAATGATACAATTGATACTT+TGG	0.330035	1.1:+64627503	MS.gene005525:CDS
TGTTGTTAGTGTCAGCAATA+AGG	0.334363	1.1:-64626767	None:intergenic
GGTTGCTTCACAATTACAAC+TGG	0.349891	1.1:-64627962	None:intergenic
AAAGAGAGAACGGAAGATTA+TGG	0.358619	1.1:+64626689	MS.gene005525:CDS
CCAATTTGTAGGGCACTACT+TGG	0.389332	1.1:+64627902	MS.gene005525:intron
AGAGCATGTATGCACTTTCC+TGG	0.390396	1.1:+64626733	MS.gene005525:CDS
ACCTTCTGCTGTCTACTCAT+TGG	0.404228	1.1:+64626821	MS.gene005525:CDS
ATAGAGAAAGTAGTTGGTAC+TGG	0.447004	1.1:+64627711	MS.gene005525:CDS
GTCTACTCATTGGATTCTGC+AGG	0.448519	1.1:+64626831	MS.gene005525:CDS
GCAACGCAGCAAAGAAACTA+GGG	0.514798	1.1:+64627754	MS.gene005525:CDS
TGCAACGCAGCAAAGAAACT+AGG	0.519057	1.1:+64627753	MS.gene005525:CDS
ACCTTAGTCATGGGAAGTCA+TGG	0.528245	1.1:+64627783	MS.gene005525:CDS
GCCATGACTTCCCATGACTA+AGG	0.537843	1.1:-64627784	None:intergenic
GTCATGGGAAGTCATGGCTA+TGG	0.540428	1.1:+64627789	MS.gene005525:CDS
ATAAATATAGAGAAAGTAGT+TGG	0.543839	1.1:+64627705	MS.gene005525:CDS
GGTAGCTGTTGATGAGAGTG+AGG	0.545867	1.1:+64626710	MS.gene005525:CDS
TCCAATGAGTAGACAGCAGA+AGG	0.550516	1.1:-64626822	None:intergenic
AGGGGCTGATACCTTAGTCA+TGG	0.560207	1.1:+64627773	MS.gene005525:CDS
ATGGCTATGGATTCATCAAG+AGG	0.623962	1.1:+64627802	MS.gene005525:CDS
ACAAAATGGAAAAGAGAGAA+CGG	0.623967	1.1:+64626679	None:intergenic
GGGGCTGATACCTTAGTCAT+GGG	0.624939	1.1:+64627774	MS.gene005525:CDS
AATAAGGTTACTGATAGACC+AGG	0.625419	1.1:-64626751	None:intergenic
AATGAGTAGACAGCAGAAGG+TGG	0.668557	1.1:-64626819	None:intergenic
GTTGCTTCACAATTACAACT+GGG	0.683814	1.1:-64627961	None:intergenic
CAACGCAGCAAAGAAACTAG+GGG	0.702473	1.1:+64627755	MS.gene005525:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGTTTATTAAATACTAATT+TGG	+	chr1.1:64627449-64627468	MS.gene005525:intron	10.0%
!!!	ATTAAGTTTTATTAGTAAAA+AGG	-	chr1.1:64626995-64627014	None:intergenic	10.0%
!!	ATTAACATTAAACACATAAT+TGG	+	chr1.1:64626878-64626897	MS.gene005525:intron	15.0%
!!	TTATGTGTTTAATGTTAATA+AGG	-	chr1.1:64626877-64626896	None:intergenic	15.0%
!!!	AAACTAATATTTTTGTTGAT+AGG	+	chr1.1:64627016-64627035	MS.gene005525:intron	15.0%
!!!	ACTTATTTTTGAAGATTATT+GGG	-	chr1.1:64626920-64626939	None:intergenic	15.0%
!!!	TTTTAGAATATACAAAATGA+TGG	+	chr1.1:64627859-64627878	MS.gene005525:intron	15.0%
!!	ATAAATATAGAGAAAGTAGT+TGG	+	chr1.1:64627705-64627724	MS.gene005525:CDS	20.0%
!!	ATAATCTTCAAAAATAAGTG+AGG	+	chr1.1:64626921-64626940	MS.gene005525:intron	20.0%
!!	TATGTAATTACTATTACTCT+TGG	+	chr1.1:64627414-64627433	MS.gene005525:intron	20.0%
!!!	CACTTATTTTTGAAGATTAT+TGG	-	chr1.1:64626921-64626940	None:intergenic	20.0%
!!!	GACTTGGTATTATTATTTAT+AGG	+	chr1.1:64627384-64627403	MS.gene005525:intron	20.0%
!!!	TGGTAGTAATATATTTTACA+GGG	+	chr1.1:64627469-64627488	MS.gene005525:intron	20.0%
!!!	TTGGTAGTAATATATTTTAC+AGG	+	chr1.1:64627468-64627487	MS.gene005525:intron	20.0%
!	AAAGTGAAAAAAAATTGAGC+TGG	-	chr1.1:64627620-64627639	None:intergenic	25.0%
!	CAATGATACAATTGATACTT+TGG	+	chr1.1:64627503-64627522	MS.gene005525:CDS	25.0%
!!	CAAAGTATCAATTGTATCAT+TGG	-	chr1.1:64627505-64627524	None:intergenic	25.0%
!!	GATGGTATAACTAACTTTTA+TGG	+	chr1.1:64627877-64627896	MS.gene005525:intron	25.0%
!!	TAAGTCAATATATGTTGACT+TGG	+	chr1.1:64627333-64627352	MS.gene005525:intron	25.0%
!!!	CTTATTTTATAAGCTCTTCA+AGG	-	chr1.1:64627229-64627248	None:intergenic	25.0%
!!!	GAAATAGGTTTTGATATATG+AGG	+	chr1.1:64626947-64626966	MS.gene005525:intron	25.0%
!!!	TTTTTTTTGAAGGAAAGACT+TGG	+	chr1.1:64627368-64627387	MS.gene005525:intron	25.0%
	AAATAAGTGAGGCATGAAAT+AGG	+	chr1.1:64626932-64626951	MS.gene005525:intron	30.0%
	ACTCATAGACATGTTATGAA+CGG	+	chr1.1:64627258-64627277	MS.gene005525:intron	30.0%
!!	ACACATAATTGGTCTTATTC+AGG	+	chr1.1:64626889-64626908	MS.gene005525:intron	30.0%
!!	TTTTATGGTGCCAATTTGTA+GGG	+	chr1.1:64627892-64627911	MS.gene005525:intron	30.0%
	AAAGAGAGAACGGAAGATTA+TGG	+	chr1.1:64626689-64626708	MS.gene005525:CDS	35.0%
	AATAAGGTTACTGATAGACC+AGG	-	chr1.1:64626754-64626773	None:intergenic	35.0%
	CGAAATAAGCTGATGTTGTT+TGG	-	chr1.1:64627298-64627317	None:intergenic	35.0%
	GTTGCTTCACAATTACAACT+GGG	-	chr1.1:64627964-64627983	None:intergenic	35.0%
	TGTTGTTAGTGTCAGCAATA+AGG	-	chr1.1:64626770-64626789	None:intergenic	35.0%
	TTGTTCATCATAACAAGTCG+CGG	-	chr1.1:64627079-64627098	None:intergenic	35.0%
!	ATAGAGAAAGTAGTTGGTAC+TGG	+	chr1.1:64627711-64627730	MS.gene005525:CDS	35.0%
!!	CTTTTATGGTGCCAATTTGT+AGG	+	chr1.1:64627891-64627910	MS.gene005525:intron	35.0%
	ATGGCTATGGATTCATCAAG+AGG	+	chr1.1:64627802-64627821	MS.gene005525:CDS	40.0%
	CACAATTACAACTGGGCATT+TGG	-	chr1.1:64627957-64627976	None:intergenic	40.0%
	CACTAAAGCCATCTCGTTTA+AGG	-	chr1.1:64627042-64627061	None:intergenic	40.0%
	GGTTGCTTCACAATTACAAC+TGG	-	chr1.1:64627965-64627984	None:intergenic	40.0%
	TTGATAGGCCTTAAACGAGA+TGG	+	chr1.1:64627031-64627050	MS.gene005525:intron	40.0%
!	GTCTTTTTGTCTGCAATACG+AGG	+	chr1.1:64626969-64626988	MS.gene005525:intron	40.0%
!!	CTTAAACGAGATGGCTTTAG+TGG	+	chr1.1:64627040-64627059	MS.gene005525:intron	40.0%
	AATGAGTAGACAGCAGAAGG+TGG	-	chr1.1:64626822-64626841	None:intergenic	45.0%
	ACCTTAGTCATGGGAAGTCA+TGG	+	chr1.1:64627783-64627802	MS.gene005525:CDS	45.0%
	ACCTTCTGCTGTCTACTCAT+TGG	+	chr1.1:64626821-64626840	MS.gene005525:CDS	45.0%
	AGAGCATGTATGCACTTTCC+TGG	+	chr1.1:64626733-64626752	MS.gene005525:CDS	45.0%
	CAACGCAGCAAAGAAACTAG+GGG	+	chr1.1:64627755-64627774	MS.gene005525:CDS	45.0%
	CCAAGTAGTGCCCTACAAAT+TGG	-	chr1.1:64627905-64627924	None:intergenic	45.0%
	GCAACGCAGCAAAGAAACTA+GGG	+	chr1.1:64627754-64627773	MS.gene005525:CDS	45.0%
	GTCTACTCATTGGATTCTGC+AGG	+	chr1.1:64626831-64626850	MS.gene005525:CDS	45.0%
	TCCAATGAGTAGACAGCAGA+AGG	-	chr1.1:64626825-64626844	None:intergenic	45.0%
	TGCAACGCAGCAAAGAAACT+AGG	+	chr1.1:64627753-64627772	MS.gene005525:CDS	45.0%
!	CCAATTTGTAGGGCACTACT+TGG	+	chr1.1:64627902-64627921	MS.gene005525:intron	45.0%
!	GCTTTAGTGGCCTTACCTTT+AGG	+	chr1.1:64627053-64627072	MS.gene005525:intron	45.0%
!!!	TTATTTTTTTTTTTTTTTGA+AGG	+	chr1.1:64627358-64627377	MS.gene005525:intron	5.0%
	AGGGGCTGATACCTTAGTCA+TGG	+	chr1.1:64627773-64627792	MS.gene005525:CDS	50.0%
	CATAACAAGTCGCGGCCTAA+AGG	-	chr1.1:64627071-64627090	None:intergenic	50.0%
	GCCATGACTTCCCATGACTA+AGG	-	chr1.1:64627787-64627806	None:intergenic	50.0%
	GGGGCTGATACCTTAGTCAT+GGG	+	chr1.1:64627774-64627793	MS.gene005525:CDS	50.0%
	GTCATGGGAAGTCATGGCTA+TGG	+	chr1.1:64627789-64627808	MS.gene005525:CDS	50.0%
!	GGTAGCTGTTGATGAGAGTG+AGG	+	chr1.1:64626710-64626729	MS.gene005525:CDS	50.0%
	CAAGTCGCGGCCTAAAGGTA+AGG	-	chr1.1:64627066-64627085	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	64626684	64627988	64626684	ID=MS.gene005525
chr1.1	mRNA	64626684	64627988	64626684	ID=MS.gene005525.t1;Parent=MS.gene005525
chr1.1	exon	64626684	64626852	64626684	ID=MS.gene005525.t1.exon1;Parent=MS.gene005525.t1
chr1.1	CDS	64626684	64626852	64626684	ID=cds.MS.gene005525.t1;Parent=MS.gene005525.t1
chr1.1	exon	64627490	64627602	64627490	ID=MS.gene005525.t1.exon2;Parent=MS.gene005525.t1
chr1.1	CDS	64627490	64627602	64627490	ID=cds.MS.gene005525.t1;Parent=MS.gene005525.t1
chr1.1	exon	64627705	64627823	64627705	ID=MS.gene005525.t1.exon3;Parent=MS.gene005525.t1
chr1.1	CDS	64627705	64627823	64627705	ID=cds.MS.gene005525.t1;Parent=MS.gene005525.t1
chr1.1	exon	64627913	64627988	64627913	ID=MS.gene005525.t1.exon4;Parent=MS.gene005525.t1
chr1.1	CDS	64627913	64627988	64627913	ID=cds.MS.gene005525.t1;Parent=MS.gene005525.t1

Gene Sequence

>MS.gene005525

ATGGAAAAGAGAGAACGGAAGATTATGGTAGCTGTTGATGAGAGTGAGGAGAGCATGTATGCACTTTCCTGGTCTATCAGTAACCTTATTGCTGACACTAACAACAATAACAAGCTTGTGCTTCTCTATGTCAAGCCACCTTCTGCTGTCTACTCATTGGATTCTGCAGGTTAATTTCTTCTCACTCACTCCTTATTAACATTAAACACATAATTGGTCTTATTCAGGATCGACCCAATAATCTTCAAAAATAAGTGAGGCATGAAATAGGTTTTGATATATGAGGTCTTTTTGTCTGCAATACGAGGCCTTTTTACTAATAAAACTTAATAAAACTAATATTTTTGTTGATAGGCCTTAAACGAGATGGCTTTAGTGGCCTTACCTTTAGGCCGCGACTTGTTATGATGAACAACTTAATTAAAAGCTTATTGAATAAACTTTTAATATATAAGTGCTCATGTAAAAGTAATTTTCATAACAAAAATATAAAATAAAGATTAACTGTTTTCATATAAATTATAAGTTGATTTTATAAGCTACCTTGAAGAGCTTATAAAATAAGTTAAAAACAACTCATAGACATGTTATGAACGGTTTCATTAGTTCTTCCAAACAACATCAGCTTATTTCGCTAGATAAACTCAAATAAGTCAATATATGTTGACTTGGTATTATTTTTTTTTTTTTTTGAAGGAAAGACTTGGTATTATTATTTATAGGATATTATTATGTAATTACTATTACTCTTGGCTTTTGAGTAACATGTTTATTAAATACTAATTTGGTAGTAATATATTTTACAGGGTATATTTTTTCCAATGATACAATTGATACTTTGGAAAACTATAGTTCACAGTTAGCTAAATCAGTAATGAAAAGAGCTGAAGCTATTTACAGAAACTTTGACGATGCAGATGTAAGTCAACTCATCCAGCTCAATTTTTTTTCACTTTTTTTTTTTAAAAAAAAATTCTTCAATTTTTAGTTGTTGACTCTGAATTTGAGATTTTATTTACAGATAAATATAGAGAAAGTAGTTGGTACTGGAGATGCTAAGAATGTTATATGCAACGCAGCAAAGAAACTAGGGGCTGATACCTTAGTCATGGGAAGTCATGGCTATGGATTCATCAAGAGGTAATTAACATTAACATATATAGTCTTTAGTTATTTTTTAGAATATACAAAATGATGGTATAACTAACTTTTATGGTGCCAATTTGTAGGGCACTACTTGGAAGTGTAAGTGATTACTGTGTCAAGAATGCCAAATGCCCAGTTGTAATTGTGAAGCAACCATAG

Protein sequence

>MS.gene005525.t1

MEKRERKIMVAVDESEESMYALSWSISNLIADTNNNNKLVLLYVKPPSAVYSLDSAGYIFSNDTIDTLENYSSQLAKSVMKRAEAIYRNFDDADINIEKVVGTGDAKNVICNAAKKLGADTLVMGSHGYGFIKRALLGSVSDYCVKNAKCPVVIVKQP