AlfalfaGEDB | MS.gene73234

M. sativa cultivar XinJiangDaYe / MS.gene73234

KEGG: -
GO: -
PFAM: PF00582.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73234.t1	XP_013454933.1	99.3	135	1	0	1	135	1	135	1.60E-69	271.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73234.t1	Q8VYN9	31.8	129	75	4	7	129	50	171	2.6e-07	56.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73234.t1	B7FGP5	99.3	135	1	0	1	135	1	135	1.2e-69	271.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene73234	0.805795	1.12E-49	-1.69E-46
MS.gene049283	MS.gene73234	0.819953	8.86E-53	-1.69E-46
MS.gene049499	MS.gene73234	0.803847	2.87E-49	-1.69E-46
MS.gene049580	MS.gene73234	0.811731	6.05E-51	-1.69E-46
MS.gene050559	MS.gene73234	0.804084	2.56E-49	-1.69E-46
MS.gene050752	MS.gene73234	0.80743	5.07E-50	-1.69E-46
MS.gene050945	MS.gene73234	0.80544	1.33E-49	-1.69E-46
MS.gene050955	MS.gene73234	0.807514	4.87E-50	-1.69E-46
MS.gene051343	MS.gene73234	0.806223	9.13E-50	-1.69E-46
MS.gene051597	MS.gene73234	0.820639	6.17E-53	-1.69E-46
MS.gene051699	MS.gene73234	0.810858	9.35E-51	-1.69E-46
MS.gene05188	MS.gene73234	0.808298	3.32E-50	-1.69E-46
MS.gene051903	MS.gene73234	0.802895	4.52E-49	-1.69E-46
MS.gene051975	MS.gene73234	0.810856	9.36E-51	-1.69E-46
MS.gene052108	MS.gene73234	0.800685	1.28E-48	-1.69E-46
MS.gene052479	MS.gene73234	0.80044	1.44E-48	-1.69E-46
MS.gene052500	MS.gene73234	0.813013	3.17E-51	-1.69E-46
MS.gene052502	MS.gene73234	0.814069	1.86E-51	-1.69E-46
MS.gene052826	MS.gene73234	0.812217	4.74E-51	-1.69E-46
MS.gene052947	MS.gene73234	0.819204	1.31E-52	-1.69E-46
MS.gene052954	MS.gene73234	0.841229	5.39E-58	-1.69E-46
MS.gene053006	MS.gene73234	0.817165	3.80E-52	-1.69E-46
MS.gene053045	MS.gene73234	0.817387	3.39E-52	-1.69E-46
MS.gene053294	MS.gene73234	0.804263	2.35E-49	-1.69E-46
MS.gene053931	MS.gene73234	0.820897	5.38E-53	-1.69E-46
MS.gene053963	MS.gene73234	0.85478	9.80E-62	-1.69E-46
MS.gene054222	MS.gene73234	0.808941	2.42E-50	-1.69E-46
MS.gene054328	MS.gene73234	0.840309	9.40E-58	-1.69E-46
MS.gene054403	MS.gene73234	0.812915	3.33E-51	-1.69E-46
MS.gene05469	MS.gene73234	0.807144	5.83E-50	-1.69E-46
MS.gene05548	MS.gene73234	0.829402	5.25E-55	-1.69E-46
MS.gene055555	MS.gene73234	0.827044	1.94E-54	-1.69E-46
MS.gene055808	MS.gene73234	0.818124	2.31E-52	-1.69E-46
MS.gene055841	MS.gene73234	0.822271	2.59E-53	-1.69E-46
MS.gene056091	MS.gene73234	0.814079	1.85E-51	-1.69E-46
MS.gene05689	MS.gene73234	0.820925	5.30E-53	-1.69E-46
MS.gene056986	MS.gene73234	0.824959	6.08E-54	-1.69E-46
MS.gene057079	MS.gene73234	0.826082	3.30E-54	-1.69E-46
MS.gene057102	MS.gene73234	0.816679	4.88E-52	-1.69E-46
MS.gene057208	MS.gene73234	0.837359	5.45E-57	-1.69E-46
MS.gene057234	MS.gene73234	0.802114	6.54E-49	-1.69E-46
MS.gene057392	MS.gene73234	0.83032	3.14E-55	-1.69E-46
MS.gene057694	MS.gene73234	0.830941	2.21E-55	-1.69E-46
MS.gene057705	MS.gene73234	0.825442	4.68E-54	-1.69E-46
MS.gene05781	MS.gene73234	0.811419	7.07E-51	-1.69E-46
MS.gene05795	MS.gene73234	0.833047	6.67E-56	-1.69E-46
MS.gene057999	MS.gene73234	0.829198	5.89E-55	-1.69E-46
MS.gene058008	MS.gene73234	0.828236	1.01E-54	-1.69E-46
MS.gene058009	MS.gene73234	0.815672	8.20E-52	-1.69E-46
MS.gene058054	MS.gene73234	0.804244	2.37E-49	-1.69E-46
MS.gene058074	MS.gene73234	0.802999	4.30E-49	-1.69E-46
MS.gene058159	MS.gene73234	0.821659	3.59E-53	-1.69E-46
MS.gene058238	MS.gene73234	0.816663	4.92E-52	-1.69E-46
MS.gene058498	MS.gene73234	0.812828	3.48E-51	-1.69E-46
MS.gene059146	MS.gene73234	0.816187	6.29E-52	-1.69E-46
MS.gene059227	MS.gene73234	0.811317	7.44E-51	-1.69E-46
MS.gene059317	MS.gene73234	0.828467	8.85E-55	-1.69E-46
MS.gene05952	MS.gene73234	0.807629	4.60E-50	-1.69E-46
MS.gene059830	MS.gene73234	0.803413	3.53E-49	-1.69E-46
MS.gene05985	MS.gene73234	-0.821449	4.01E-53	-1.69E-46
MS.gene059935	MS.gene73234	0.802001	6.90E-49	-1.69E-46
MS.gene060244	MS.gene73234	0.809297	2.03E-50	-1.69E-46
MS.gene060365	MS.gene73234	0.834953	2.22E-56	-1.69E-46
MS.gene060429	MS.gene73234	0.822331	2.51E-53	-1.69E-46
MS.gene060586	MS.gene73234	0.825359	4.89E-54	-1.69E-46
MS.gene060763	MS.gene73234	0.813893	2.03E-51	-1.69E-46
MS.gene060879	MS.gene73234	0.804828	1.79E-49	-1.69E-46
MS.gene060917	MS.gene73234	0.802668	5.03E-49	-1.69E-46
MS.gene060935	MS.gene73234	0.81772	2.85E-52	-1.69E-46
MS.gene060937	MS.gene73234	0.817833	2.69E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene07276	MS.gene73234	PPI
MS.gene73234	MS.gene20798	PPI
MS.gene73234	MS.gene032573	PPI
MS.gene73234	MS.gene07213	PPI
MS.gene73234	MS.gene050004	PPI
MS.gene73234	MS.gene017358	PPI
MS.gene73234	MS.gene00161	PPI
MS.gene73234	MS.gene06986	PPI
MS.gene73234	MS.gene063633	PPI
MS.gene22971	MS.gene73234	PPI
MS.gene73234	MS.gene053006	PPI
MS.gene73234	MS.gene005602	PPI
MS.gene73234	MS.gene058230	PPI
MS.gene26154	MS.gene73234	PPI
MS.gene73234	MS.gene20731	PPI
MS.gene73234	MS.gene82881	PPI
MS.gene73234	MS.gene009525	PPI
MS.gene73234	MS.gene010109	PPI
MS.gene070229	MS.gene73234	PPI
MS.gene73234	MS.gene005525	PPI
MS.gene20848	MS.gene73234	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73234.t1	MTR_4g019800	97.101	138	4	0	1	138	1	138	9.18e-97	276
MS.gene73234.t1	MTR_7g065770	66.667	138	46	0	1	138	1	138	1.07e-67	202
MS.gene73234.t1	MTR_4g064933	63.768	138	50	0	1	138	1	138	1.18e-61	187
MS.gene73234.t1	MTR_5g077130	52.899	138	65	0	1	138	1	138	5.06e-48	152
MS.gene73234.t1	MTR_5g077130	52.518	139	65	1	1	138	1	139	8.82e-47	149
MS.gene73234.t1	MTR_2g034200	45.926	135	73	0	1	135	1	135	5.72e-36	122
MS.gene73234.t1	MTR_5g065980	34.965	143	77	5	9	138	12	151	1.74e-12	61.6
MS.gene73234.t1	MTR_5g065980	34.266	143	76	5	9	138	12	149	6.49e-12	60.1
MS.gene73234.t1	MTR_6g038080	73.529	34	9	0	95	128	12	45	2.33e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73234.t1	AT3G53990	74.638	138	35	0	1	138	1	138	4.87e-73	216
MS.gene73234.t1	AT3G03270	55.797	138	61	0	1	138	1	138	8.78e-49	154
MS.gene73234.t1	AT3G53990	74.490	98	25	0	1	98	1	98	1.41e-47	150
MS.gene73234.t1	AT3G03270	57.037	135	58	0	1	135	1	135	4.55e-47	151
MS.gene73234.t1	AT3G17020	47.407	135	70	1	5	138	7	141	1.21e-43	141
MS.gene73234.t1	AT3G17020	47.253	91	47	1	5	94	7	97	4.27e-25	92.8

Find 26 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAGCATTCTTGCTGCTCT+TGG	0.307367	4.1:-78422672	None:intergenic
GAAGCTGGATTCTCTTGTTA+TGG	0.310746	4.1:+78423453	MS.gene73234:CDS
TAGAACTATTGGTGTGGCTT+TGG	0.330939	4.1:+78422643	MS.gene73234:CDS
TATTGTTACAAAAGTGTATT+GGG	0.331126	4.1:+78423390	MS.gene73234:CDS
AAGCTGGATTCTCTTGTTAT+GGG	0.360643	4.1:+78423454	MS.gene73234:CDS
GCAGCAAGAATGCTCTGAAA+TGG	0.370068	4.1:+78422678	MS.gene73234:CDS
ATTCTCTTGTTATGGGTAGC+AGG	0.383547	4.1:+78423461	MS.gene73234:CDS
CCATACTTCTTCATTATCTC+TGG	0.389368	4.1:-78423224	None:intergenic
ATGGCAAAAGATAGAACTAT+TGG	0.392250	4.1:+78422632	MS.gene73234:CDS
GTACTTACGAGAACCATCTT+TGG	0.412789	4.1:-78422792	None:intergenic
ATGAAGCTCGTAATCAGTTA+TGG	0.420885	4.1:+78422768	MS.gene73234:CDS
TTCTCTTGTTATGGGTAGCA+GGG	0.424515	4.1:+78423462	MS.gene73234:CDS
ATATTGTTACAAAAGTGTAT+TGG	0.434652	4.1:+78423389	MS.gene73234:CDS
CCAGAGATAATGAAGAAGTA+TGG	0.442632	4.1:+78423224	MS.gene73234:CDS
CAGCAAGAATGCTCTGAAAT+GGG	0.466495	4.1:+78422679	MS.gene73234:CDS
TGAAGCTCGTAATCAGTTAT+GGG	0.466658	4.1:+78422769	MS.gene73234:CDS
TGATGCAAGAGAGAAACTTA+TGG	0.472999	4.1:+78423414	MS.gene73234:CDS
TGCTGTTGAAGATCTGAAGC+TGG	0.501707	4.1:+78423438	MS.gene73234:CDS
GGTTCTCTGAACTCTTTCAA+AGG	0.548832	4.1:-78423203	None:intergenic
GGGGTCTCAGCACAATTCAA+AGG	0.550843	4.1:+78423482	MS.gene73234:CDS
AATCAGTTATGGGCCAAAGA+TGG	0.569005	4.1:+78422779	MS.gene73234:CDS
TCTCTTGTTATGGGTAGCAG+GGG	0.589338	4.1:+78423463	MS.gene73234:CDS
CTTGAGAATTTAGCTGACAA+AGG	0.596595	4.1:+78422704	MS.gene73234:CDS
TGGTGTTCAGATTGATATCG+AGG	0.609478	4.1:+78423244	MS.gene73234:CDS
ATTGTTACAAAAGTGTATTG+GGG	0.627971	4.1:+78423391	MS.gene73234:CDS
AAAAGATAGAACTATTGGTG+TGG	0.655375	4.1:+78422637	MS.gene73234:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGTAATAATTATTGATTTTT+AGG	+	chr4.1:78423363-78423382	MS.gene73234:intron	10.0%
!!	AAAAATTAACGAAAAATGAA+TGG	-	chr4.1:78422817-78422836	None:intergenic	15.0%
!!	AATGTAATTCTTAGAAATCA+TGG	+	chr4.1:78422977-78422996	MS.gene73234:intron	20.0%
!!	TCAAACTAACAATATAAGAT+CGG	-	chr4.1:78422871-78422890	None:intergenic	20.0%
!!!	ATATTGTTACAAAAGTGTAT+TGG	+	chr4.1:78423389-78423408	MS.gene73234:CDS	20.0%
!!!	TATTGTTACAAAAGTGTATT+GGG	+	chr4.1:78423390-78423409	MS.gene73234:CDS	20.0%
!	AATTCTTAGAAATCATGGTT+GGG	+	chr4.1:78422982-78423001	MS.gene73234:intron	25.0%
!	TAATTCTTAGAAATCATGGT+TGG	+	chr4.1:78422981-78423000	MS.gene73234:intron	25.0%
!!	AATTTAGACAAGGATGTTTT+AGG	+	chr4.1:78423329-78423348	MS.gene73234:intron	25.0%
!!	ATTGTTACAAAAGTGTATTG+GGG	+	chr4.1:78423391-78423410	MS.gene73234:CDS	25.0%
!!!	TCTGCATTTTAATTTAGACA+AGG	+	chr4.1:78423319-78423338	MS.gene73234:intron	25.0%
	AAAAGATAGAACTATTGGTG+TGG	+	chr4.1:78422637-78422656	MS.gene73234:CDS	30.0%
	ATGGCAAAAGATAGAACTAT+TGG	+	chr4.1:78422632-78422651	MS.gene73234:CDS	30.0%
!!	AACTCATAAACCGATTTTGT+AGG	-	chr4.1:78423019-78423038	None:intergenic	30.0%
!!	ACTCATAAACCGATTTTGTA+GGG	-	chr4.1:78423018-78423037	None:intergenic	30.0%
!!!	CACTTTTTCAAGACAAAAAG+AGG	+	chr4.1:78423283-78423302	MS.gene73234:CDS	30.0%
	ATGAAGCTCGTAATCAGTTA+TGG	+	chr4.1:78422768-78422787	MS.gene73234:CDS	35.0%
	CCAGAGATAATGAAGAAGTA+TGG	+	chr4.1:78423224-78423243	MS.gene73234:CDS	35.0%
	CCATACTTCTTCATTATCTC+TGG	-	chr4.1:78423227-78423246	None:intergenic	35.0%
	CTAACACAACCCTACAAAAT+CGG	+	chr4.1:78423006-78423025	MS.gene73234:intron	35.0%
	CTTGAGAATTTAGCTGACAA+AGG	+	chr4.1:78422704-78422723	MS.gene73234:CDS	35.0%
	GATAACAGAAATCTGATAGC+AGG	+	chr4.1:78423080-78423099	MS.gene73234:intron	35.0%
	GCTATCAGATTTCTGTTATC+AGG	-	chr4.1:78423080-78423099	None:intergenic	35.0%
	TGAAGCTCGTAATCAGTTAT+GGG	+	chr4.1:78422769-78422788	MS.gene73234:CDS	35.0%
	TGATGCAAGAGAGAAACTTA+TGG	+	chr4.1:78423414-78423433	MS.gene73234:CDS	35.0%
!	AAGCTGGATTCTCTTGTTAT+GGG	+	chr4.1:78423454-78423473	MS.gene73234:CDS	35.0%
	AACAGAAATCTGATAGCAGG+TGG	+	chr4.1:78423083-78423102	MS.gene73234:intron	40.0%
	AATCAGTTATGGGCCAAAGA+TGG	+	chr4.1:78422779-78422798	MS.gene73234:CDS	40.0%
	CTCCACTTATAAATCGTGGT+GGG	+	chr4.1:78423051-78423070	MS.gene73234:intron	40.0%
	GAAGCTGGATTCTCTTGTTA+TGG	+	chr4.1:78423453-78423472	MS.gene73234:CDS	40.0%
	GGTTCTCTGAACTCTTTCAA+AGG	-	chr4.1:78423206-78423225	None:intergenic	40.0%
	GTACTTACGAGAACCATCTT+TGG	-	chr4.1:78422795-78422814	None:intergenic	40.0%
	TAGAACTATTGGTGTGGCTT+TGG	+	chr4.1:78422643-78422662	MS.gene73234:CDS	40.0%
	TGGTGTTCAGATTGATATCG+AGG	+	chr4.1:78423244-78423263	MS.gene73234:CDS	40.0%
	TTGCCTCCACTTATAAATCG+TGG	+	chr4.1:78423047-78423066	MS.gene73234:intron	40.0%
!	ATTCTCTTGTTATGGGTAGC+AGG	+	chr4.1:78423461-78423480	MS.gene73234:CDS	40.0%
!	TTCTCTTGTTATGGGTAGCA+GGG	+	chr4.1:78423462-78423481	MS.gene73234:CDS	40.0%
!!	CAGCAAGAATGCTCTGAAAT+GGG	+	chr4.1:78422679-78422698	MS.gene73234:CDS	40.0%
	CACCCACCACGATTTATAAG+TGG	-	chr4.1:78423056-78423075	None:intergenic	45.0%
	CCACCACGATTTATAAGTGG+AGG	-	chr4.1:78423053-78423072	None:intergenic	45.0%
	CCTCCACTTATAAATCGTGG+TGG	+	chr4.1:78423050-78423069	MS.gene73234:intron	45.0%
!	CACTTATAAATCGTGGTGGG+TGG	+	chr4.1:78423054-78423073	MS.gene73234:intron	45.0%
!	TCTCTTGTTATGGGTAGCAG+GGG	+	chr4.1:78423463-78423482	MS.gene73234:CDS	45.0%
!	TGCTGTTGAAGATCTGAAGC+TGG	+	chr4.1:78423438-78423457	MS.gene73234:CDS	45.0%
!!	GCAGCAAGAATGCTCTGAAA+TGG	+	chr4.1:78422678-78422697	MS.gene73234:CDS	45.0%
	AGAGCCTCTCAAGATCCACT+AGG	-	chr4.1:78423109-78423128	None:intergenic	50.0%
	GGGGTCTCAGCACAATTCAA+AGG	+	chr4.1:78423482-78423501	MS.gene73234:CDS	50.0%
!	CAGAGCATTCTTGCTGCTCT+TGG	-	chr4.1:78422675-78422694	None:intergenic	50.0%
!	GAGAGGCTCTGATAGCATTC+TGG	+	chr4.1:78423119-78423138	MS.gene73234:intron	50.0%
	TCTGATAGCAGGTGGCCTAG+TGG	+	chr4.1:78423091-78423110	MS.gene73234:intron	55.0%
!	GTGGCCTAGTGGATCTTGAG+AGG	+	chr4.1:78423102-78423121	MS.gene73234:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	78422632	78423528	78422632	ID=MS.gene73234
chr4.1	mRNA	78422632	78423528	78422632	ID=MS.gene73234.t1;Parent=MS.gene73234
chr4.1	exon	78422632	78422806	78422632	ID=MS.gene73234.t1.exon1;Parent=MS.gene73234.t1
chr4.1	CDS	78422632	78422806	78422632	ID=cds.MS.gene73234.t1;Parent=MS.gene73234.t1
chr4.1	exon	78423195	78423304	78423195	ID=MS.gene73234.t1.exon2;Parent=MS.gene73234.t1
chr4.1	CDS	78423195	78423304	78423195	ID=cds.MS.gene73234.t1;Parent=MS.gene73234.t1
chr4.1	exon	78423385	78423528	78423385	ID=MS.gene73234.t1.exon3;Parent=MS.gene73234.t1
chr4.1	CDS	78423385	78423528	78423385	ID=cds.MS.gene73234.t1;Parent=MS.gene73234.t1

Gene Sequence

>MS.gene73234

ATGGCAAAAGATAGAACTATTGGTGTGGCTTTGGATTTCTCCAAGAGCAGCAAGAATGCTCTGAAATGGGCACTTGAGAATTTAGCTGACAAAGGTGACAACATTTACATCATTCACATCAGTCATGATTCACTTGATGAAGCTCGTAATCAGTTATGGGCCAAAGATGGTTCTCGTAAGTACCATTCATTTTTCGTTAATTTTTTACTTATCTTATGTTTTATATTTTTTCTGATCCGATCTTATATTGTTAGTTTGATAATAGTTCGAATCTTGATTGAAATAATTCTTGATCAAACTTTACTTATCATCAGACTGAATTTTAGATTATCAGAGTCTCATATAAATGTAATTCTTAGAAATCATGGTTGGGTCTAACACAACCCTACAAAATCGGTTTATGAGTTGAGAAGAATTGCCTCCACTTATAAATCGTGGTGGGTGGCCTGATAACAGAAATCTGATAGCAGGTGGCCTAGTGGATCTTGAGAGGCTCTGATAGCATTCTGGATATGATATTGATATTGATTTTTTTTTTTTTTTTTTTGTGTGTGTGTGTGAAGCTCTTATTCCTTTGAAAGAGTTCAGAGAACCAGAGATAATGAAGAAGTATGGTGTTCAGATTGATATCGAGGTTCTTGATTTGCTTGACACTTTTTCAAGACAAAAAGAGGTATTTTTTATTTTTCTGCATTTTAATTTAGACAAGGATGTTTTAGGAGTTTTAATTGTGTAATAATTATTGATTTTTAGGTGAATATTGTTACAAAAGTGTATTGGGGTGATGCAAGAGAGAAACTTATGGATGCTGTTGAAGATCTGAAGCTGGATTCTCTTGTTATGGGTAGCAGGGGTCTCAGCACAATTCAAAGGTTTTTTTTTTTTTTTTTACCTTAA

Protein sequence

>MS.gene73234.t1

MAKDRTIGVALDFSKSSKNALKWALENLADKGDNIYIIHISHDSLDEARNQLWAKDGSPLIPLKEFREPEIMKKYGVQIDIEVLDLLDTFSRQKEVNIVTKVYWGDAREKLMDAVEDLKLDSLVMGSRGLSTIQRFFFFFLP