Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07276.t1 | XP_003591417.1 | 98.7 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 1.30E-80 | 308.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07276.t1 | Q8VYN9 | 31.9 | 163 | 94 | 4 | 7 | 158 | 49 | 205 | 8.7e-12 | 71.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07276.t1 | G7IDR7 | 98.7 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 9.7e-81 | 308.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07276 | MS.gene73234 | PPI |
| MS.gene07276 | MS.gene005602 | PPI |
| MS.gene07276 | MS.gene06986 | PPI |
| MS.gene07276 | MS.gene22971 | PPI |
| MS.gene07276 | MS.gene009525 | PPI |
| MS.gene07276 | MS.gene010109 | PPI |
| MS.gene07276 | MS.gene26154 | PPI |
| MS.gene07276 | MS.gene20731 | PPI |
| MS.gene048106 | MS.gene07276 | PPI |
| MS.gene07276 | MS.gene037687 | PPI |
| MS.gene07276 | MS.gene92557 | PPI |
| MS.gene07276 | MS.gene07213 | PPI |
| MS.gene07276 | MS.gene050004 | PPI |
| MS.gene07276 | MS.gene017358 | PPI |
| MS.gene07276 | MS.gene063633 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07276.t1 | MTR_1g087200 | 98.734 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 1.66e-114 | 321 |
| MS.gene07276.t1 | MTR_1g083950 | 52.597 | 154 | 67 | 3 | 6 | 158 | 9 | 157 | 7.02e-56 | 173 |
| MS.gene07276.t1 | MTR_1g088640 | 39.375 | 160 | 91 | 4 | 2 | 158 | 7 | 163 | 8.12e-33 | 114 |
| MS.gene07276.t1 | MTR_1g088640 | 39.873 | 158 | 90 | 4 | 2 | 158 | 7 | 160 | 1.64e-31 | 111 |
| MS.gene07276.t1 | MTR_1g054750 | 34.706 | 170 | 95 | 5 | 2 | 158 | 17 | 183 | 1.64e-30 | 109 |
| MS.gene07276.t1 | MTR_1g054765 | 36.471 | 170 | 87 | 5 | 7 | 158 | 4 | 170 | 1.67e-29 | 106 |
| MS.gene07276.t1 | MTR_7g108830 | 41.026 | 156 | 87 | 4 | 5 | 158 | 6 | 158 | 4.98e-27 | 99.8 |
| MS.gene07276.t1 | MTR_8g058620 | 41.026 | 156 | 87 | 4 | 5 | 158 | 6 | 158 | 4.98e-27 | 99.8 |
| MS.gene07276.t1 | MTR_5g065980 | 35.185 | 162 | 91 | 4 | 8 | 156 | 10 | 170 | 1.03e-26 | 99.4 |
| MS.gene07276.t1 | MTR_5g065980 | 35.185 | 162 | 89 | 4 | 8 | 156 | 10 | 168 | 1.24e-26 | 99.0 |
| MS.gene07276.t1 | MTR_5g077130 | 36.774 | 155 | 84 | 5 | 10 | 156 | 9 | 157 | 1.58e-21 | 85.5 |
| MS.gene07276.t1 | MTR_5g077130 | 36.538 | 156 | 84 | 6 | 10 | 156 | 9 | 158 | 2.43e-20 | 82.4 |
| MS.gene07276.t1 | MTR_2g089350 | 32.738 | 168 | 89 | 6 | 6 | 158 | 25 | 183 | 2.48e-20 | 84.0 |
| MS.gene07276.t1 | MTR_1g088620 | 58.462 | 65 | 25 | 1 | 94 | 158 | 220 | 282 | 4.37e-18 | 79.0 |
| MS.gene07276.t1 | MTR_4g019800 | 33.129 | 163 | 91 | 6 | 4 | 156 | 3 | 157 | 3.10e-17 | 74.3 |
| MS.gene07276.t1 | MTR_6g006190 | 27.168 | 173 | 100 | 4 | 1 | 157 | 1 | 163 | 9.42e-17 | 73.6 |
| MS.gene07276.t1 | MTR_2g034200 | 28.481 | 158 | 101 | 3 | 6 | 156 | 5 | 157 | 3.67e-16 | 71.6 |
| MS.gene07276.t1 | MTR_4g064933 | 35.404 | 161 | 88 | 5 | 6 | 157 | 5 | 158 | 1.12e-15 | 70.5 |
| MS.gene07276.t1 | MTR_5g065980 | 32.394 | 142 | 80 | 4 | 8 | 136 | 10 | 148 | 1.51e-15 | 70.9 |
| MS.gene07276.t1 | MTR_7g065770 | 29.091 | 165 | 95 | 4 | 4 | 156 | 3 | 157 | 2.60e-13 | 64.3 |
| MS.gene07276.t1 | MTR_6g006190 | 39.683 | 63 | 38 | 0 | 95 | 157 | 77 | 139 | 3.18e-12 | 60.8 |
| MS.gene07276.t1 | MTR_1717s0010 | 28.571 | 161 | 89 | 5 | 6 | 143 | 42 | 199 | 1.27e-11 | 60.8 |
| MS.gene07276.t1 | MTR_1527s0010 | 28.571 | 161 | 89 | 5 | 6 | 143 | 42 | 199 | 1.27e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07276.t1 | AT3G62550 | 61.146 | 157 | 58 | 3 | 2 | 157 | 3 | 157 | 5.33e-64 | 193 |
| MS.gene07276.t1 | AT3G11930 | 36.970 | 165 | 89 | 4 | 6 | 158 | 33 | 194 | 3.38e-29 | 106 |
| MS.gene07276.t1 | AT3G11930 | 36.747 | 166 | 89 | 4 | 6 | 158 | 33 | 195 | 4.59e-29 | 105 |
| MS.gene07276.t1 | AT3G11930 | 36.527 | 167 | 89 | 4 | 6 | 158 | 33 | 196 | 6.35e-29 | 105 |
| MS.gene07276.t1 | AT2G47710 | 39.869 | 153 | 86 | 4 | 8 | 158 | 10 | 158 | 1.06e-27 | 101 |
| MS.gene07276.t1 | AT3G58450 | 35.802 | 162 | 90 | 5 | 8 | 158 | 32 | 190 | 3.17e-27 | 101 |
| MS.gene07276.t1 | AT1G09740 | 37.975 | 158 | 86 | 4 | 8 | 156 | 10 | 164 | 8.72e-27 | 99.4 |
| MS.gene07276.t1 | AT1G09740 | 37.975 | 158 | 86 | 4 | 8 | 156 | 10 | 164 | 8.72e-27 | 99.4 |
| MS.gene07276.t1 | AT3G58450 | 36.076 | 158 | 88 | 4 | 8 | 158 | 32 | 183 | 9.82e-27 | 100 |
| MS.gene07276.t1 | AT1G68300 | 35.714 | 154 | 90 | 3 | 6 | 156 | 10 | 157 | 4.20e-26 | 97.4 |
| MS.gene07276.t1 | AT1G11360 | 35.802 | 162 | 93 | 4 | 5 | 158 | 37 | 195 | 8.51e-24 | 93.6 |
| MS.gene07276.t1 | AT1G11360 | 35.802 | 162 | 93 | 4 | 5 | 158 | 37 | 195 | 8.51e-24 | 93.6 |
| MS.gene07276.t1 | AT1G11360 | 35.802 | 162 | 93 | 4 | 5 | 158 | 37 | 195 | 8.51e-24 | 93.6 |
| MS.gene07276.t1 | AT1G11360 | 35.802 | 162 | 93 | 4 | 5 | 158 | 37 | 195 | 8.51e-24 | 93.6 |
| MS.gene07276.t1 | AT3G11930 | 31.771 | 192 | 89 | 5 | 6 | 158 | 33 | 221 | 4.84e-23 | 91.3 |
| MS.gene07276.t1 | AT5G14680 | 26.506 | 166 | 110 | 3 | 1 | 157 | 1 | 163 | 2.71e-18 | 77.4 |
| MS.gene07276.t1 | AT3G01520 | 28.000 | 175 | 96 | 5 | 1 | 157 | 1 | 163 | 2.21e-17 | 75.1 |
| MS.gene07276.t1 | AT3G03270 | 30.357 | 168 | 89 | 4 | 4 | 156 | 3 | 157 | 1.17e-15 | 70.1 |
| MS.gene07276.t1 | AT3G53990 | 27.215 | 158 | 107 | 2 | 4 | 156 | 3 | 157 | 3.88e-15 | 68.9 |
| MS.gene07276.t1 | AT3G17020 | 29.518 | 166 | 98 | 4 | 2 | 156 | 3 | 160 | 8.91e-15 | 68.2 |
Find 27 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAATTACAACTGGGCATT+TGG | 0.240443 | 1.4:-69504515 | None:intergenic |
| CAAAGTATCAATTGTATCAT+TGG | 0.275490 | 1.4:-69504064 | None:intergenic |
| AAAGAGAGAGCGGAAGATTA+TGG | 0.304990 | 1.4:+69503280 | MS.gene07276:CDS |
| CAATGATACAATTGATACTT+TGG | 0.330035 | 1.4:+69504065 | MS.gene07276:CDS |
| TGTTGTTAGTGTCAGCAATA+AGG | 0.334363 | 1.4:-69503358 | None:intergenic |
| GGTTGCTTCACAATTACAAC+TGG | 0.349891 | 1.4:-69504523 | None:intergenic |
| CCAATTTGTAGGGCACTACT+TGG | 0.389332 | 1.4:+69504463 | MS.gene07276:intron |
| AGAGCATGTATGCACTTTCC+TGG | 0.390396 | 1.4:+69503324 | MS.gene07276:CDS |
| ACCTTCTGCTGTCTACTCAT+TGG | 0.404228 | 1.4:+69503412 | MS.gene07276:CDS |
| GGTAGCAGTTGATGAGAGTG+AGG | 0.430921 | 1.4:+69503301 | MS.gene07276:CDS |
| ATAGAGAAAGTAGTTGGTAC+TGG | 0.447004 | 1.4:+69504272 | MS.gene07276:CDS |
| GTCTACTCATTGGATTCTGC+AGG | 0.448519 | 1.4:+69503422 | MS.gene07276:CDS |
| GCAACGCAGCAAAGAAACTA+GGG | 0.514798 | 1.4:+69504315 | MS.gene07276:CDS |
| TGCAACGCAGCAAAGAAACT+AGG | 0.519057 | 1.4:+69504314 | MS.gene07276:CDS |
| ACCTTAGTCATGGGAAGTCA+TGG | 0.528245 | 1.4:+69504344 | MS.gene07276:CDS |
| GCCATGACTTCCCATGACTA+AGG | 0.537843 | 1.4:-69504345 | None:intergenic |
| GTCATGGGAAGTCATGGCTA+TGG | 0.540428 | 1.4:+69504350 | MS.gene07276:CDS |
| ATAAATATAGAGAAAGTAGT+TGG | 0.543839 | 1.4:+69504266 | MS.gene07276:CDS |
| TCCAATGAGTAGACAGCAGA+AGG | 0.550516 | 1.4:-69503413 | None:intergenic |
| AGGGGCTGATACCTTAGTCA+TGG | 0.560207 | 1.4:+69504334 | MS.gene07276:CDS |
| ATGGCTATGGATTCATCAAG+AGG | 0.623962 | 1.4:+69504363 | MS.gene07276:CDS |
| GGGGCTGATACCTTAGTCAT+GGG | 0.624939 | 1.4:+69504335 | MS.gene07276:CDS |
| AATAAGGTTACTGATAGACC+AGG | 0.625419 | 1.4:-69503342 | None:intergenic |
| AATGAGTAGACAGCAGAAGG+TGG | 0.668557 | 1.4:-69503410 | None:intergenic |
| ACAAAATGGAAAAGAGAGAG+CGG | 0.669276 | 1.4:+69503270 | None:intergenic |
| GTTGCTTCACAATTACAACT+GGG | 0.683814 | 1.4:-69504522 | None:intergenic |
| CAACGCAGCAAAGAAACTAG+GGG | 0.702473 | 1.4:+69504316 | MS.gene07276:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAAGTTTTATTAGTAAAA+AGG | - | chr1.4:69503592-69503611 | None:intergenic | 10.0% |
| !! | ATGTTTATTGAATACTAATT+TGG | + | chr1.4:69504011-69504030 | MS.gene07276:intron | 15.0% |
| !! | TAAAACATTAAACACATAAT+TGG | + | chr1.4:69503471-69503490 | MS.gene07276:intron | 15.0% |
| !!! | AAACTAATATTTTTGTTGAT+AGG | + | chr1.4:69503613-69503632 | MS.gene07276:intron | 15.0% |
| !!! | ATGTGTTTAATGTTTTAATA+AGG | - | chr1.4:69503468-69503487 | None:intergenic | 15.0% |
| !!! | TTTTAGAATATACAAAATGA+TGG | + | chr1.4:69504420-69504439 | MS.gene07276:intron | 15.0% |
| !! | ATAAATATAGAGAAAGTAGT+TGG | + | chr1.4:69504266-69504285 | MS.gene07276:CDS | 20.0% |
| !! | TATGTAATTACTATTACTCT+TGG | + | chr1.4:69503976-69503995 | MS.gene07276:intron | 20.0% |
| !!! | ACTTATTTCTGAAGATTATT+GGG | - | chr1.4:69503513-69503532 | None:intergenic | 20.0% |
| !!! | GACTTGGTATTATTATTTAT+AGG | + | chr1.4:69503946-69503965 | MS.gene07276:intron | 20.0% |
| !!! | TAGGTTTTGATATATATATG+AGG | + | chr1.4:69503544-69503563 | MS.gene07276:intron | 20.0% |
| !!! | TGGTAGTAATATATTTTACA+GGG | + | chr1.4:69504031-69504050 | MS.gene07276:intron | 20.0% |
| !!! | TTGGTAGTAATATATTTTAC+AGG | + | chr1.4:69504030-69504049 | MS.gene07276:intron | 20.0% |
| ! | AAAGTGAAAAAAAATTGAGC+TGG | - | chr1.4:69504182-69504201 | None:intergenic | 25.0% |
| ! | ATAATCTTCAGAAATAAGTG+AGG | + | chr1.4:69503514-69503533 | MS.gene07276:intron | 25.0% |
| ! | CAATGATACAATTGATACTT+TGG | + | chr1.4:69504065-69504084 | MS.gene07276:CDS | 25.0% |
| !! | ACAAATAAGTTGTTGTTGTT+TGG | - | chr1.4:69503895-69503914 | None:intergenic | 25.0% |
| !! | CAAAGTATCAATTGTATCAT+TGG | - | chr1.4:69504067-69504086 | None:intergenic | 25.0% |
| !! | GATGGTATAACTAACTTTTA+TGG | + | chr1.4:69504438-69504457 | MS.gene07276:intron | 25.0% |
| !! | TAAGTCAATATATGTTGACT+TGG | + | chr1.4:69503930-69503949 | MS.gene07276:intron | 25.0% |
| !!! | CACTTATTTCTGAAGATTAT+TGG | - | chr1.4:69503514-69503533 | None:intergenic | 25.0% |
| !!! | CTTATTTTATAAGCTCTTCA+AGG | - | chr1.4:69503826-69503845 | None:intergenic | 25.0% |
| AAATAAGTGAGGCATGAAAT+AGG | + | chr1.4:69503525-69503544 | MS.gene07276:intron | 30.0% | |
| ACTCATAGACATGTTATGAA+CGG | + | chr1.4:69503855-69503874 | MS.gene07276:intron | 30.0% | |
| !! | ACACATAATTGGTCTTATTC+AGG | + | chr1.4:69503482-69503501 | MS.gene07276:intron | 30.0% |
| !! | TAATTGGTCTTATTCAGGAT+CGG | + | chr1.4:69503487-69503506 | MS.gene07276:intron | 30.0% |
| !! | TTTTATGGTGCCAATTTGTA+GGG | + | chr1.4:69504453-69504472 | MS.gene07276:intron | 30.0% |
| AATAAGGTTACTGATAGACC+AGG | - | chr1.4:69503345-69503364 | None:intergenic | 35.0% | |
| GTTGCTTCACAATTACAACT+GGG | - | chr1.4:69504525-69504544 | None:intergenic | 35.0% | |
| TGTTGTTAGTGTCAGCAATA+AGG | - | chr1.4:69503361-69503380 | None:intergenic | 35.0% | |
| TTGTTCATCATAACAAGTCG+CGG | - | chr1.4:69503676-69503695 | None:intergenic | 35.0% | |
| ! | ATAGAGAAAGTAGTTGGTAC+TGG | + | chr1.4:69504272-69504291 | MS.gene07276:CDS | 35.0% |
| !! | CTTTTATGGTGCCAATTTGT+AGG | + | chr1.4:69504452-69504471 | MS.gene07276:intron | 35.0% |
| AAAGAGAGAGCGGAAGATTA+TGG | + | chr1.4:69503280-69503299 | MS.gene07276:CDS | 40.0% | |
| ATGGCTATGGATTCATCAAG+AGG | + | chr1.4:69504363-69504382 | MS.gene07276:CDS | 40.0% | |
| CACAATTACAACTGGGCATT+TGG | - | chr1.4:69504518-69504537 | None:intergenic | 40.0% | |
| GGTTGCTTCACAATTACAAC+TGG | - | chr1.4:69504526-69504545 | None:intergenic | 40.0% | |
| TTGATAGGCCTTAAACGAGA+GGG | + | chr1.4:69503628-69503647 | MS.gene07276:intron | 40.0% | |
| ! | GTCTTTTTGTCTGCAATACG+AGG | + | chr1.4:69503566-69503585 | MS.gene07276:intron | 40.0% |
| AATGAGTAGACAGCAGAAGG+TGG | - | chr1.4:69503413-69503432 | None:intergenic | 45.0% | |
| ACCTTAGTCATGGGAAGTCA+TGG | + | chr1.4:69504344-69504363 | MS.gene07276:CDS | 45.0% | |
| ACCTTCTGCTGTCTACTCAT+TGG | + | chr1.4:69503412-69503431 | MS.gene07276:CDS | 45.0% | |
| AGAGCATGTATGCACTTTCC+TGG | + | chr1.4:69503324-69503343 | MS.gene07276:CDS | 45.0% | |
| CAACGCAGCAAAGAAACTAG+GGG | + | chr1.4:69504316-69504335 | MS.gene07276:CDS | 45.0% | |
| CACTAAAGCCCTCTCGTTTA+AGG | - | chr1.4:69503639-69503658 | None:intergenic | 45.0% | |
| CCAAGTAGTGCCCTACAAAT+TGG | - | chr1.4:69504466-69504485 | None:intergenic | 45.0% | |
| GCAACGCAGCAAAGAAACTA+GGG | + | chr1.4:69504315-69504334 | MS.gene07276:CDS | 45.0% | |
| GTCTACTCATTGGATTCTGC+AGG | + | chr1.4:69503422-69503441 | MS.gene07276:CDS | 45.0% | |
| TCCAATGAGTAGACAGCAGA+AGG | - | chr1.4:69503416-69503435 | None:intergenic | 45.0% | |
| TGCAACGCAGCAAAGAAACT+AGG | + | chr1.4:69504314-69504333 | MS.gene07276:CDS | 45.0% | |
| ! | CCAATTTGTAGGGCACTACT+TGG | + | chr1.4:69504463-69504482 | MS.gene07276:intron | 45.0% |
| ! | CTTAAACGAGAGGGCTTTAG+TGG | + | chr1.4:69503637-69503656 | MS.gene07276:intron | 45.0% |
| ! | GCTTTAGTGGCCTTACCTTT+AGG | + | chr1.4:69503650-69503669 | MS.gene07276:intron | 45.0% |
| ! | GTTGATAGGCCTTAAACGAG+AGG | + | chr1.4:69503627-69503646 | MS.gene07276:intron | 45.0% |
| AGGGGCTGATACCTTAGTCA+TGG | + | chr1.4:69504334-69504353 | MS.gene07276:CDS | 50.0% | |
| CATAACAAGTCGCGGCCTAA+AGG | - | chr1.4:69503668-69503687 | None:intergenic | 50.0% | |
| GCCATGACTTCCCATGACTA+AGG | - | chr1.4:69504348-69504367 | None:intergenic | 50.0% | |
| GGGGCTGATACCTTAGTCAT+GGG | + | chr1.4:69504335-69504354 | MS.gene07276:CDS | 50.0% | |
| GTCATGGGAAGTCATGGCTA+TGG | + | chr1.4:69504350-69504369 | MS.gene07276:CDS | 50.0% | |
| !! | GGTAGCAGTTGATGAGAGTG+AGG | + | chr1.4:69503301-69503320 | MS.gene07276:CDS | 50.0% |
| CAAGTCGCGGCCTAAAGGTA+AGG | - | chr1.4:69503663-69503682 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 69503275 | 69504549 | 69503275 | ID=MS.gene07276 |
| chr1.4 | mRNA | 69503275 | 69504549 | 69503275 | ID=MS.gene07276.t1;Parent=MS.gene07276 |
| chr1.4 | exon | 69503275 | 69503443 | 69503275 | ID=MS.gene07276.t1.exon1;Parent=MS.gene07276.t1 |
| chr1.4 | CDS | 69503275 | 69503443 | 69503275 | ID=cds.MS.gene07276.t1;Parent=MS.gene07276.t1 |
| chr1.4 | exon | 69504052 | 69504164 | 69504052 | ID=MS.gene07276.t1.exon2;Parent=MS.gene07276.t1 |
| chr1.4 | CDS | 69504052 | 69504164 | 69504052 | ID=cds.MS.gene07276.t1;Parent=MS.gene07276.t1 |
| chr1.4 | exon | 69504266 | 69504384 | 69504266 | ID=MS.gene07276.t1.exon3;Parent=MS.gene07276.t1 |
| chr1.4 | CDS | 69504266 | 69504384 | 69504266 | ID=cds.MS.gene07276.t1;Parent=MS.gene07276.t1 |
| chr1.4 | exon | 69504474 | 69504549 | 69504474 | ID=MS.gene07276.t1.exon4;Parent=MS.gene07276.t1 |
| chr1.4 | CDS | 69504474 | 69504549 | 69504474 | ID=cds.MS.gene07276.t1;Parent=MS.gene07276.t1 |
| Gene Sequence |
| Protein sequence |
