Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20848.t1 | XP_003591417.1 | 98.7 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 1.30E-80 | 308.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20848.t1 | Q8VYN9 | 31.9 | 163 | 94 | 4 | 7 | 158 | 49 | 205 | 8.7e-12 | 71.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20848.t1 | G7IDR7 | 98.7 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 9.7e-81 | 308.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20848 | MS.gene005602 | PPI |
| MS.gene20848 | MS.gene010109 | PPI |
| MS.gene048106 | MS.gene20848 | PPI |
| MS.gene20848 | MS.gene20731 | PPI |
| MS.gene20848 | MS.gene009525 | PPI |
| MS.gene20848 | MS.gene92557 | PPI |
| MS.gene26154 | MS.gene20848 | PPI |
| MS.gene20848 | MS.gene063633 | PPI |
| MS.gene20848 | MS.gene037687 | PPI |
| MS.gene20848 | MS.gene07213 | PPI |
| MS.gene20848 | MS.gene050004 | PPI |
| MS.gene20848 | MS.gene017358 | PPI |
| MS.gene20848 | MS.gene06986 | PPI |
| MS.gene20848 | MS.gene73234 | PPI |
| MS.gene22971 | MS.gene20848 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20848.t1 | MTR_1g087200 | 98.734 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 1.66e-114 | 321 |
| MS.gene20848.t1 | MTR_1g083950 | 52.597 | 154 | 67 | 3 | 6 | 158 | 9 | 157 | 7.02e-56 | 173 |
| MS.gene20848.t1 | MTR_1g088640 | 39.375 | 160 | 91 | 4 | 2 | 158 | 7 | 163 | 8.12e-33 | 114 |
| MS.gene20848.t1 | MTR_1g088640 | 39.873 | 158 | 90 | 4 | 2 | 158 | 7 | 160 | 1.64e-31 | 111 |
| MS.gene20848.t1 | MTR_1g054750 | 34.706 | 170 | 95 | 5 | 2 | 158 | 17 | 183 | 1.64e-30 | 109 |
| MS.gene20848.t1 | MTR_1g054765 | 36.471 | 170 | 87 | 5 | 7 | 158 | 4 | 170 | 1.67e-29 | 106 |
| MS.gene20848.t1 | MTR_7g108830 | 41.026 | 156 | 87 | 4 | 5 | 158 | 6 | 158 | 4.98e-27 | 99.8 |
| MS.gene20848.t1 | MTR_8g058620 | 41.026 | 156 | 87 | 4 | 5 | 158 | 6 | 158 | 4.98e-27 | 99.8 |
| MS.gene20848.t1 | MTR_5g065980 | 35.185 | 162 | 91 | 4 | 8 | 156 | 10 | 170 | 1.03e-26 | 99.4 |
| MS.gene20848.t1 | MTR_5g065980 | 35.185 | 162 | 89 | 4 | 8 | 156 | 10 | 168 | 1.24e-26 | 99.0 |
| MS.gene20848.t1 | MTR_5g077130 | 36.774 | 155 | 84 | 5 | 10 | 156 | 9 | 157 | 1.58e-21 | 85.5 |
| MS.gene20848.t1 | MTR_5g077130 | 36.538 | 156 | 84 | 6 | 10 | 156 | 9 | 158 | 2.43e-20 | 82.4 |
| MS.gene20848.t1 | MTR_2g089350 | 32.738 | 168 | 89 | 6 | 6 | 158 | 25 | 183 | 2.48e-20 | 84.0 |
| MS.gene20848.t1 | MTR_1g088620 | 58.462 | 65 | 25 | 1 | 94 | 158 | 220 | 282 | 4.37e-18 | 79.0 |
| MS.gene20848.t1 | MTR_4g019800 | 33.129 | 163 | 91 | 6 | 4 | 156 | 3 | 157 | 3.10e-17 | 74.3 |
| MS.gene20848.t1 | MTR_6g006190 | 27.168 | 173 | 100 | 4 | 1 | 157 | 1 | 163 | 9.42e-17 | 73.6 |
| MS.gene20848.t1 | MTR_2g034200 | 28.481 | 158 | 101 | 3 | 6 | 156 | 5 | 157 | 3.67e-16 | 71.6 |
| MS.gene20848.t1 | MTR_4g064933 | 35.404 | 161 | 88 | 5 | 6 | 157 | 5 | 158 | 1.12e-15 | 70.5 |
| MS.gene20848.t1 | MTR_5g065980 | 32.394 | 142 | 80 | 4 | 8 | 136 | 10 | 148 | 1.51e-15 | 70.9 |
| MS.gene20848.t1 | MTR_7g065770 | 29.091 | 165 | 95 | 4 | 4 | 156 | 3 | 157 | 2.60e-13 | 64.3 |
| MS.gene20848.t1 | MTR_6g006190 | 39.683 | 63 | 38 | 0 | 95 | 157 | 77 | 139 | 3.18e-12 | 60.8 |
| MS.gene20848.t1 | MTR_1717s0010 | 28.571 | 161 | 89 | 5 | 6 | 143 | 42 | 199 | 1.27e-11 | 60.8 |
| MS.gene20848.t1 | MTR_1527s0010 | 28.571 | 161 | 89 | 5 | 6 | 143 | 42 | 199 | 1.27e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20848.t1 | AT3G62550 | 61.146 | 157 | 58 | 3 | 2 | 157 | 3 | 157 | 5.33e-64 | 193 |
| MS.gene20848.t1 | AT3G11930 | 36.970 | 165 | 89 | 4 | 6 | 158 | 33 | 194 | 3.38e-29 | 106 |
| MS.gene20848.t1 | AT3G11930 | 36.747 | 166 | 89 | 4 | 6 | 158 | 33 | 195 | 4.59e-29 | 105 |
| MS.gene20848.t1 | AT3G11930 | 36.527 | 167 | 89 | 4 | 6 | 158 | 33 | 196 | 6.35e-29 | 105 |
| MS.gene20848.t1 | AT2G47710 | 39.869 | 153 | 86 | 4 | 8 | 158 | 10 | 158 | 1.06e-27 | 101 |
| MS.gene20848.t1 | AT3G58450 | 35.802 | 162 | 90 | 5 | 8 | 158 | 32 | 190 | 3.17e-27 | 101 |
| MS.gene20848.t1 | AT1G09740 | 37.975 | 158 | 86 | 4 | 8 | 156 | 10 | 164 | 8.72e-27 | 99.4 |
| MS.gene20848.t1 | AT1G09740 | 37.975 | 158 | 86 | 4 | 8 | 156 | 10 | 164 | 8.72e-27 | 99.4 |
| MS.gene20848.t1 | AT3G58450 | 36.076 | 158 | 88 | 4 | 8 | 158 | 32 | 183 | 9.82e-27 | 100 |
| MS.gene20848.t1 | AT1G68300 | 35.714 | 154 | 90 | 3 | 6 | 156 | 10 | 157 | 4.20e-26 | 97.4 |
| MS.gene20848.t1 | AT1G11360 | 35.802 | 162 | 93 | 4 | 5 | 158 | 37 | 195 | 8.51e-24 | 93.6 |
| MS.gene20848.t1 | AT1G11360 | 35.802 | 162 | 93 | 4 | 5 | 158 | 37 | 195 | 8.51e-24 | 93.6 |
| MS.gene20848.t1 | AT1G11360 | 35.802 | 162 | 93 | 4 | 5 | 158 | 37 | 195 | 8.51e-24 | 93.6 |
| MS.gene20848.t1 | AT1G11360 | 35.802 | 162 | 93 | 4 | 5 | 158 | 37 | 195 | 8.51e-24 | 93.6 |
| MS.gene20848.t1 | AT3G11930 | 31.771 | 192 | 89 | 5 | 6 | 158 | 33 | 221 | 4.84e-23 | 91.3 |
| MS.gene20848.t1 | AT5G14680 | 26.506 | 166 | 110 | 3 | 1 | 157 | 1 | 163 | 2.71e-18 | 77.4 |
| MS.gene20848.t1 | AT3G01520 | 28.000 | 175 | 96 | 5 | 1 | 157 | 1 | 163 | 2.21e-17 | 75.1 |
| MS.gene20848.t1 | AT3G03270 | 30.357 | 168 | 89 | 4 | 4 | 156 | 3 | 157 | 1.17e-15 | 70.1 |
| MS.gene20848.t1 | AT3G53990 | 27.215 | 158 | 107 | 2 | 4 | 156 | 3 | 157 | 3.88e-15 | 68.9 |
| MS.gene20848.t1 | AT3G17020 | 29.518 | 166 | 98 | 4 | 2 | 156 | 3 | 160 | 8.91e-15 | 68.2 |
Find 27 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAATTACAACTGGGCATT+TGG | 0.240443 | 1.3:-61700290 | None:intergenic |
| CAAAGTATCAATTGTATCAT+TGG | 0.275490 | 1.3:-61699839 | None:intergenic |
| AAAGAGAGAGCGGAAGATTA+TGG | 0.304990 | 1.3:+61699055 | MS.gene20848:CDS |
| CAATGATACAATTGATACTT+TGG | 0.330035 | 1.3:+61699840 | MS.gene20848:CDS |
| TGTTGTTAGTGTCAGCAATA+AGG | 0.334363 | 1.3:-61699133 | None:intergenic |
| GGTTGCTTCACAATTACAAC+TGG | 0.349891 | 1.3:-61700298 | None:intergenic |
| CCAATTTGTAGGGCACTACT+TGG | 0.389332 | 1.3:+61700238 | MS.gene20848:intron |
| AGAGCATGTATGCACTTTCC+TGG | 0.390396 | 1.3:+61699099 | MS.gene20848:CDS |
| ACCTTCTGCTGTCTACTCAT+TGG | 0.404228 | 1.3:+61699187 | MS.gene20848:CDS |
| GGTAGCAGTTGATGAGAGTG+AGG | 0.430921 | 1.3:+61699076 | MS.gene20848:CDS |
| ATAGAGAAAGTAGTTGGTAC+TGG | 0.447004 | 1.3:+61700047 | MS.gene20848:CDS |
| GTCTACTCATTGGATTCTGC+AGG | 0.448519 | 1.3:+61699197 | MS.gene20848:CDS |
| GCAACGCAGCAAAGAAACTA+GGG | 0.514798 | 1.3:+61700090 | MS.gene20848:CDS |
| TGCAACGCAGCAAAGAAACT+AGG | 0.519057 | 1.3:+61700089 | MS.gene20848:CDS |
| ACCTTAGTCATGGGAAGTCA+TGG | 0.528245 | 1.3:+61700119 | MS.gene20848:CDS |
| GCCATGACTTCCCATGACTA+AGG | 0.537843 | 1.3:-61700120 | None:intergenic |
| GTCATGGGAAGTCATGGCTA+TGG | 0.540428 | 1.3:+61700125 | MS.gene20848:CDS |
| ATAAATATAGAGAAAGTAGT+TGG | 0.543839 | 1.3:+61700041 | MS.gene20848:CDS |
| TCCAATGAGTAGACAGCAGA+AGG | 0.550516 | 1.3:-61699188 | None:intergenic |
| AGGGGCTGATACCTTAGTCA+TGG | 0.560207 | 1.3:+61700109 | MS.gene20848:CDS |
| ATGGCTATGGATTCATCAAG+AGG | 0.623962 | 1.3:+61700138 | MS.gene20848:CDS |
| GGGGCTGATACCTTAGTCAT+GGG | 0.624939 | 1.3:+61700110 | MS.gene20848:CDS |
| AATAAGGTTACTGATAGACC+AGG | 0.625419 | 1.3:-61699117 | None:intergenic |
| AATGAGTAGACAGCAGAAGG+TGG | 0.668557 | 1.3:-61699185 | None:intergenic |
| ACAAAATGGAAAAGAGAGAG+CGG | 0.669276 | 1.3:+61699045 | None:intergenic |
| GTTGCTTCACAATTACAACT+GGG | 0.683814 | 1.3:-61700297 | None:intergenic |
| CAACGCAGCAAAGAAACTAG+GGG | 0.702473 | 1.3:+61700091 | MS.gene20848:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAAGTTTTATTAGTAAAA+AGG | - | chr1.3:61699367-61699386 | None:intergenic | 10.0% |
| !! | ATGTTTATTGAATACTAATT+TGG | + | chr1.3:61699786-61699805 | MS.gene20848:intron | 15.0% |
| !! | TAAAACATTAAACACATAAT+TGG | + | chr1.3:61699246-61699265 | MS.gene20848:intron | 15.0% |
| !!! | AAACTAATATTTTTGTTGAT+AGG | + | chr1.3:61699388-61699407 | MS.gene20848:intron | 15.0% |
| !!! | ATGTGTTTAATGTTTTAATA+AGG | - | chr1.3:61699243-61699262 | None:intergenic | 15.0% |
| !!! | TTTTAGAATATACAAAATGA+TGG | + | chr1.3:61700195-61700214 | MS.gene20848:intron | 15.0% |
| !! | ATAAATATAGAGAAAGTAGT+TGG | + | chr1.3:61700041-61700060 | MS.gene20848:CDS | 20.0% |
| !! | TATGTAATTACTATTACTCT+TGG | + | chr1.3:61699751-61699770 | MS.gene20848:intron | 20.0% |
| !!! | GACTTGGTATTATTATTTAT+AGG | + | chr1.3:61699721-61699740 | MS.gene20848:intron | 20.0% |
| !!! | TAGGTTTTGATATATATATG+AGG | + | chr1.3:61699319-61699338 | MS.gene20848:intron | 20.0% |
| !!! | TGGTAGTAATATATTTTACA+GGG | + | chr1.3:61699806-61699825 | MS.gene20848:intron | 20.0% |
| !!! | TTGGTAGTAATATATTTTAC+AGG | + | chr1.3:61699805-61699824 | MS.gene20848:intron | 20.0% |
| ! | AAAGTGAAAAAAAATTGAGC+TGG | - | chr1.3:61699957-61699976 | None:intergenic | 25.0% |
| ! | CAATGATACAATTGATACTT+TGG | + | chr1.3:61699840-61699859 | MS.gene20848:CDS | 25.0% |
| !! | ACAAATAAGTTGTTGTTGTT+TGG | - | chr1.3:61699670-61699689 | None:intergenic | 25.0% |
| !! | CAAAGTATCAATTGTATCAT+TGG | - | chr1.3:61699842-61699861 | None:intergenic | 25.0% |
| !! | GATGGTATAACTAACTTTTA+TGG | + | chr1.3:61700213-61700232 | MS.gene20848:intron | 25.0% |
| !! | TAAGTCAATATATGTTGACT+TGG | + | chr1.3:61699705-61699724 | MS.gene20848:intron | 25.0% |
| !!! | ACTTATTTCTGGAGATTATT+GGG | - | chr1.3:61699288-61699307 | None:intergenic | 25.0% |
| !!! | CTTATTTTATAAGCTCTTCA+AGG | - | chr1.3:61699601-61699620 | None:intergenic | 25.0% |
| AAATAAGTGAGGCATGAAAT+AGG | + | chr1.3:61699300-61699319 | MS.gene20848:intron | 30.0% | |
| ACTCATAGACATGTTATGAA+CGG | + | chr1.3:61699630-61699649 | MS.gene20848:intron | 30.0% | |
| ATAATCTCCAGAAATAAGTG+AGG | + | chr1.3:61699289-61699308 | MS.gene20848:intron | 30.0% | |
| !! | ACACATAATTGGTCTTATTC+AGG | + | chr1.3:61699257-61699276 | MS.gene20848:intron | 30.0% |
| !! | CACTTATTTCTGGAGATTAT+TGG | - | chr1.3:61699289-61699308 | None:intergenic | 30.0% |
| !! | TAATTGGTCTTATTCAGGAT+CGG | + | chr1.3:61699262-61699281 | MS.gene20848:intron | 30.0% |
| !! | TTTTATGGTGCCAATTTGTA+GGG | + | chr1.3:61700228-61700247 | MS.gene20848:intron | 30.0% |
| AATAAGGTTACTGATAGACC+AGG | - | chr1.3:61699120-61699139 | None:intergenic | 35.0% | |
| GTTGCTTCACAATTACAACT+GGG | - | chr1.3:61700300-61700319 | None:intergenic | 35.0% | |
| TGTTGTTAGTGTCAGCAATA+AGG | - | chr1.3:61699136-61699155 | None:intergenic | 35.0% | |
| TTGTTCATCATAACAAGTCG+CGG | - | chr1.3:61699451-61699470 | None:intergenic | 35.0% | |
| TTTCATGCCTCACTTATTTC+TGG | - | chr1.3:61699299-61699318 | None:intergenic | 35.0% | |
| ! | ATAGAGAAAGTAGTTGGTAC+TGG | + | chr1.3:61700047-61700066 | MS.gene20848:CDS | 35.0% |
| !! | CTTTTATGGTGCCAATTTGT+AGG | + | chr1.3:61700227-61700246 | MS.gene20848:intron | 35.0% |
| AAAGAGAGAGCGGAAGATTA+TGG | + | chr1.3:61699055-61699074 | MS.gene20848:CDS | 40.0% | |
| ATGGCTATGGATTCATCAAG+AGG | + | chr1.3:61700138-61700157 | MS.gene20848:CDS | 40.0% | |
| CACAATTACAACTGGGCATT+TGG | - | chr1.3:61700293-61700312 | None:intergenic | 40.0% | |
| GGTTGCTTCACAATTACAAC+TGG | - | chr1.3:61700301-61700320 | None:intergenic | 40.0% | |
| TTGATAGGCCTTAAACGAGA+GGG | + | chr1.3:61699403-61699422 | MS.gene20848:intron | 40.0% | |
| ! | GTCTTTTTGTCTGCAATACG+AGG | + | chr1.3:61699341-61699360 | MS.gene20848:intron | 40.0% |
| AATGAGTAGACAGCAGAAGG+TGG | - | chr1.3:61699188-61699207 | None:intergenic | 45.0% | |
| ACCTTAGTCATGGGAAGTCA+TGG | + | chr1.3:61700119-61700138 | MS.gene20848:CDS | 45.0% | |
| ACCTTCTGCTGTCTACTCAT+TGG | + | chr1.3:61699187-61699206 | MS.gene20848:CDS | 45.0% | |
| AGAGCATGTATGCACTTTCC+TGG | + | chr1.3:61699099-61699118 | MS.gene20848:CDS | 45.0% | |
| CAACGCAGCAAAGAAACTAG+GGG | + | chr1.3:61700091-61700110 | MS.gene20848:CDS | 45.0% | |
| CACTAAAGCCCTCTCGTTTA+AGG | - | chr1.3:61699414-61699433 | None:intergenic | 45.0% | |
| CCAAGTAGTGCCCTACAAAT+TGG | - | chr1.3:61700241-61700260 | None:intergenic | 45.0% | |
| GCAACGCAGCAAAGAAACTA+GGG | + | chr1.3:61700090-61700109 | MS.gene20848:CDS | 45.0% | |
| GTCTACTCATTGGATTCTGC+AGG | + | chr1.3:61699197-61699216 | MS.gene20848:CDS | 45.0% | |
| TCCAATGAGTAGACAGCAGA+AGG | - | chr1.3:61699191-61699210 | None:intergenic | 45.0% | |
| TGCAACGCAGCAAAGAAACT+AGG | + | chr1.3:61700089-61700108 | MS.gene20848:CDS | 45.0% | |
| ! | CCAATTTGTAGGGCACTACT+TGG | + | chr1.3:61700238-61700257 | MS.gene20848:intron | 45.0% |
| ! | CTTAAACGAGAGGGCTTTAG+TGG | + | chr1.3:61699412-61699431 | MS.gene20848:intron | 45.0% |
| ! | GCTTTAGTGGCCTTACCTTT+AGG | + | chr1.3:61699425-61699444 | MS.gene20848:intron | 45.0% |
| ! | GTTGATAGGCCTTAAACGAG+AGG | + | chr1.3:61699402-61699421 | MS.gene20848:intron | 45.0% |
| AGGGGCTGATACCTTAGTCA+TGG | + | chr1.3:61700109-61700128 | MS.gene20848:CDS | 50.0% | |
| CATAACAAGTCGCGGCCTAA+AGG | - | chr1.3:61699443-61699462 | None:intergenic | 50.0% | |
| GCCATGACTTCCCATGACTA+AGG | - | chr1.3:61700123-61700142 | None:intergenic | 50.0% | |
| GGGGCTGATACCTTAGTCAT+GGG | + | chr1.3:61700110-61700129 | MS.gene20848:CDS | 50.0% | |
| GTCATGGGAAGTCATGGCTA+TGG | + | chr1.3:61700125-61700144 | MS.gene20848:CDS | 50.0% | |
| !! | GGTAGCAGTTGATGAGAGTG+AGG | + | chr1.3:61699076-61699095 | MS.gene20848:CDS | 50.0% |
| CAAGTCGCGGCCTAAAGGTA+AGG | - | chr1.3:61699438-61699457 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 61699050 | 61700324 | 61699050 | ID=MS.gene20848 |
| chr1.3 | mRNA | 61699050 | 61700324 | 61699050 | ID=MS.gene20848.t1;Parent=MS.gene20848 |
| chr1.3 | exon | 61699050 | 61699218 | 61699050 | ID=MS.gene20848.t1.exon1;Parent=MS.gene20848.t1 |
| chr1.3 | CDS | 61699050 | 61699218 | 61699050 | ID=cds.MS.gene20848.t1;Parent=MS.gene20848.t1 |
| chr1.3 | exon | 61699827 | 61699939 | 61699827 | ID=MS.gene20848.t1.exon2;Parent=MS.gene20848.t1 |
| chr1.3 | CDS | 61699827 | 61699939 | 61699827 | ID=cds.MS.gene20848.t1;Parent=MS.gene20848.t1 |
| chr1.3 | exon | 61700041 | 61700159 | 61700041 | ID=MS.gene20848.t1.exon3;Parent=MS.gene20848.t1 |
| chr1.3 | CDS | 61700041 | 61700159 | 61700041 | ID=cds.MS.gene20848.t1;Parent=MS.gene20848.t1 |
| chr1.3 | exon | 61700249 | 61700324 | 61700249 | ID=MS.gene20848.t1.exon4;Parent=MS.gene20848.t1 |
| chr1.3 | CDS | 61700249 | 61700324 | 61700249 | ID=cds.MS.gene20848.t1;Parent=MS.gene20848.t1 |
| Gene Sequence |
| Protein sequence |
