AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006772

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006772.t1 MTR_4g081460 94.347 513 29 0 1 513 50 562 0.0 1019
MS.gene006772.t1 MTR_6g008560 54.990 511 229 1 3 513 48 557 0.0 609
MS.gene006772.t1 MTR_5g062230 55.773 511 224 1 3 513 45 553 0.0 608
MS.gene006772.t1 MTR_3g464190 56.641 512 219 2 3 513 48 557 0.0 604
MS.gene006772.t1 MTR_5g073200 56.641 512 219 2 3 513 40 549 0.0 603
MS.gene006772.t1 MTR_4g048460 56.360 511 221 1 3 513 53 561 0.0 595
MS.gene006772.t1 MTR_5g073260 54.883 512 229 2 3 513 42 552 0.0 592
MS.gene006772.t1 MTR_6g039440 53.216 513 238 1 1 513 43 553 0.0 588
MS.gene006772.t1 MTR_5g094620 53.229 511 218 2 3 513 48 537 0.0 572
MS.gene006772.t1 MTR_6g008560 56.110 401 176 0 113 513 1 401 4.93e-173 493
MS.gene006772.t1 MTR_4g048460 57.729 414 173 1 3 416 53 464 1.39e-170 490
MS.gene006772.t1 MTR_8g007515 47.287 516 263 6 3 513 45 556 7.02e-166 481
MS.gene006772.t1 MTR_2g082010 46.035 517 271 7 1 513 56 568 6.18e-165 479
MS.gene006772.t1 MTR_2g081980 45.841 517 270 8 3 513 45 557 7.13e-163 474
MS.gene006772.t1 MTR_2g082060 44.595 518 266 9 2 513 51 553 7.17e-152 445
MS.gene006772.t1 MTR_4g019550 42.205 526 271 8 2 513 52 558 3.68e-139 413
MS.gene006772.t1 MTR_2g082050 40.234 512 245 8 2 510 3 456 2.03e-127 379
MS.gene006772.t1 MTR_4g045810 38.416 505 305 4 7 510 79 578 8.45e-118 359
MS.gene006772.t1 MTR_2g065450 38.012 513 308 5 2 512 85 589 9.80e-110 338
MS.gene006772.t1 MTR_2g064425 38.207 513 306 7 2 512 82 585 1.68e-109 338
MS.gene006772.t1 MTR_8g057020 37.132 509 310 5 6 512 88 588 3.45e-108 334
MS.gene006772.t1 MTR_2g089120 36.789 492 296 6 2 490 65 544 9.23e-108 332
MS.gene006772.t1 MTR_2g089130 36.992 492 294 6 2 490 65 543 2.84e-107 331
MS.gene006772.t1 MTR_2g064425 36.647 513 299 8 2 512 82 570 9.69e-100 312
MS.gene006772.t1 MTR_4g092010 34.151 489 308 9 9 490 60 541 2.20e-90 288
MS.gene006772.t1 MTR_4g092020 35.579 475 299 4 23 493 82 553 1.64e-89 285
MS.gene006772.t1 MTR_7g010710 33.878 490 317 4 25 510 66 552 4.99e-89 283
MS.gene006772.t1 MTR_2g064425 43.807 331 178 4 2 331 82 405 2.54e-83 265
MS.gene006772.t1 MTR_2g010960 29.480 519 350 9 1 509 6 518 1.28e-77 253
MS.gene006772.t1 MTR_2g064425 41.390 331 171 5 2 331 82 390 1.00e-73 240
MS.gene006772.t1 MTR_3g465090 29.779 497 329 10 1 486 6 493 5.67e-72 238
MS.gene006772.t1 MTR_3g063120 30.638 470 307 8 30 486 6 469 3.43e-71 235
MS.gene006772.t1 MTR_3g063170 29.234 496 333 10 1 486 6 493 9.83e-70 232
MS.gene006772.t1 MTR_3g052120 29.839 496 330 9 1 486 6 493 1.47e-69 231
MS.gene006772.t1 MTR_6g064980 28.210 514 351 8 1 503 6 512 1.11e-68 229
MS.gene006772.t1 MTR_3g063140 28.932 515 346 11 1 503 6 512 4.73e-68 228
MS.gene006772.t1 MTR_5g094530 49.770 217 73 3 124 338 2 184 1.84e-64 207
MS.gene006772.t1 MTR_7g057340 50.581 172 85 0 342 513 1 172 2.04e-58 191
MS.gene006772.t1 MTR_6g065040 29.668 391 268 5 102 486 2 391 2.15e-57 196
MS.gene006772.t1 MTR_7g057390 48.837 172 85 1 342 513 1 169 8.75e-53 176
MS.gene006772.t1 MTR_7g050990 52.830 159 62 2 148 302 15 164 3.19e-47 176
MS.gene006772.t1 MTR_3g063160 30.375 293 186 6 1 285 6 288 2.08e-38 142
MS.gene006772.t1 MTR_2g064295 28.320 512 316 18 30 504 259 756 2.33e-38 150
MS.gene006772.t1 MTR_3g058160 28.544 522 314 20 30 509 68 572 1.73e-37 145
MS.gene006772.t1 MTR_3g058160 28.544 522 314 20 30 509 259 763 5.17e-37 145
MS.gene006772.t1 MTR_3g058160 28.544 522 314 20 30 509 279 783 5.50e-37 145
MS.gene006772.t1 MTR_3g058160 28.544 522 314 20 30 509 272 776 5.93e-37 145
MS.gene006772.t1 MTR_3g058160 27.808 543 312 21 30 509 279 804 2.57e-34 138
MS.gene006772.t1 MTR_3g015080 36.047 172 72 2 342 513 1 134 3.03e-30 115
MS.gene006772.t1 MTR_2g012870 23.707 464 325 11 30 470 257 714 7.46e-28 118
MS.gene006772.t1 MTR_2g012900 24.222 450 300 13 30 444 257 700 1.26e-25 111
MS.gene006772.t1 MTR_7g011663 28.296 311 178 8 16 299 335 627 3.17e-22 101
MS.gene006772.t1 MTR_7g094970 26.241 282 180 7 16 270 197 477 2.41e-21 98.2
MS.gene006772.t1 MTR_5g030050 27.203 261 157 8 30 266 3 254 5.37e-19 88.2
MS.gene006772.t1 MTR_7g011770 28.276 290 179 7 30 291 245 533 8.06e-19 90.5
MS.gene006772.t1 MTR_5g081730 39.583 96 58 0 119 214 16 111 4.53e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006772.t1 AT5G23960 44.314 510 278 4 6 513 40 545 5.00e-153 448
MS.gene006772.t1 AT5G23960 44.336 512 277 5 6 513 40 547 1.06e-151 444
MS.gene006772.t1 AT3G14490 40.272 514 297 5 5 513 93 601 6.40e-130 391
MS.gene006772.t1 AT1G70080 38.252 515 309 6 2 509 95 607 1.13e-123 375
MS.gene006772.t1 AT3G14540 36.170 517 321 4 2 513 89 601 5.98e-117 357
MS.gene006772.t1 AT1G31950 36.810 489 302 3 30 513 120 606 6.38e-116 355
MS.gene006772.t1 AT1G31950 36.810 489 302 3 30 513 121 607 6.56e-116 355
MS.gene006772.t1 AT3G14520 35.590 517 324 4 2 513 92 604 8.78e-116 354
MS.gene006772.t1 AT5G44630 37.864 515 312 6 2 510 42 554 1.31e-113 347
MS.gene006772.t1 AT3G32030 36.965 514 312 6 6 513 97 604 6.80e-113 347
MS.gene006772.t1 AT3G29410 35.812 511 320 5 6 513 98 603 1.55e-111 343
MS.gene006772.t1 AT1G33750 35.673 513 323 4 3 513 96 603 5.05e-111 342
MS.gene006772.t1 AT3G29410 35.060 502 319 4 14 513 2 498 2.41e-110 337
MS.gene006772.t1 AT4G20200 36.047 516 324 4 2 513 91 604 4.27e-108 335
MS.gene006772.t1 AT4G16730 33.080 526 330 7 2 513 22 539 1.96e-107 331
MS.gene006772.t1 AT4G16730 33.131 495 318 4 30 513 1 493 3.28e-105 323
MS.gene006772.t1 AT4G15870 34.825 514 314 7 2 509 96 594 4.53e-104 324
MS.gene006772.t1 AT4G20210 32.946 516 340 4 2 513 87 600 2.92e-101 317
MS.gene006772.t1 AT3G29190 33.719 519 330 6 3 513 89 601 1.06e-100 315
MS.gene006772.t1 AT4G13300 35.328 518 319 6 2 510 41 551 3.63e-100 312
MS.gene006772.t1 AT4G20230 34.308 513 330 6 2 509 91 601 4.56e-100 314
MS.gene006772.t1 AT4G20230 34.308 513 330 6 2 509 103 613 5.04e-100 314
MS.gene006772.t1 AT4G13280 35.409 514 324 5 2 510 41 551 2.85e-97 305
MS.gene006772.t1 AT2G23230 35.341 498 314 7 6 497 89 584 1.88e-95 302
MS.gene006772.t1 AT1G48800 34.047 514 330 6 2 509 92 602 6.32e-95 300
MS.gene006772.t1 AT1G66020 34.051 511 331 4 3 509 86 594 4.34e-94 298
MS.gene006772.t1 AT3G14540 36.643 423 260 3 2 420 89 507 1.11e-93 294
MS.gene006772.t1 AT2G23230 35.141 498 310 8 6 497 89 579 1.77e-93 296
MS.gene006772.t1 AT1G61680 33.607 488 310 6 9 490 77 556 1.94e-93 295
MS.gene006772.t1 AT3G29110 33.203 512 336 5 2 509 84 593 2.42e-92 293
MS.gene006772.t1 AT1G31950 37.596 391 238 2 30 416 121 509 1.58e-91 289
MS.gene006772.t1 AT1G61680 33.402 488 304 7 9 490 77 549 2.29e-91 290
MS.gene006772.t1 AT4G16740 32.762 525 327 11 1 513 54 564 4.79e-91 289
MS.gene006772.t1 AT3G25830 31.887 530 324 11 6 513 80 594 2.14e-90 288
MS.gene006772.t1 AT3G25820 31.887 530 324 11 6 513 80 594 2.14e-90 288
MS.gene006772.t1 AT2G24210 32.830 530 316 12 6 513 79 590 5.57e-90 287
MS.gene006772.t1 AT4G13280 33.852 514 302 7 2 510 41 521 9.56e-86 274
MS.gene006772.t1 AT3G25810 32.283 508 317 11 25 513 98 597 1.64e-85 276
MS.gene006772.t1 AT5G48110 38.484 343 206 5 172 513 1 339 5.52e-79 251
MS.gene006772.t1 AT1G48820 32.563 519 289 11 2 509 92 560 7.44e-77 252
MS.gene006772.t1 AT3G25820 34.916 358 209 6 6 350 80 426 1.86e-65 219
MS.gene006772.t1 AT4G16740 36.207 348 203 7 1 338 54 392 4.60e-65 216
MS.gene006772.t1 AT1G66020 36.778 329 202 4 3 327 86 412 1.20e-64 216
MS.gene006772.t1 AT4G16740 38.384 297 164 7 1 287 54 341 1.34e-55 191
MS.gene006772.t1 AT4G16740 38.384 297 164 7 1 287 54 341 1.34e-55 191
MS.gene006772.t1 AT3G25820 37.627 295 160 6 6 287 80 363 1.43e-55 191
MS.gene006772.t1 AT3G25820 37.627 295 160 6 6 287 80 363 1.43e-55 191
MS.gene006772.t1 AT1G79460 24.409 508 341 16 29 507 276 769 1.97e-29 123
MS.gene006772.t1 AT1G61120 22.649 521 365 14 16 510 273 781 3.12e-26 113
MS.gene006772.t1 AT4G02780 30.466 279 162 8 16 265 320 595 5.75e-24 106

Find 91 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAAATTGAAATCTAATT+TGG 0.157846 4.1:+25482366 None:intergenic
AGATGAAGCATTGACAATTT+TGG 0.158357 4.1:+25481399 None:intergenic
GATGATATATATGATGTTTA+TGG 0.206526 4.1:-25482045 MS.gene006772:CDS
CACAATAATCATGTTGAAAA+TGG 0.211729 4.1:-25483210 MS.gene006772:CDS
ATTCAATTGCACTTGTCTTT+AGG 0.255161 4.1:-25483161 MS.gene006772:CDS
CAAAGTCCCTTTACTCATAT+TGG 0.261866 4.1:+25480966 None:intergenic
AGTGCCAAAGCCTCTCCTTA+TGG 0.298277 4.1:-25481102 MS.gene006772:CDS
GTCATTCTTTGTTGATCCTT+GGG 0.305154 4.1:+25481016 None:intergenic
AGAGGCTTTGGCACTTGAAT+TGG 0.307687 4.1:+25481110 None:intergenic
GATTGATTCAATCCAACGTT+TGG 0.313958 4.1:-25483283 MS.gene006772:CDS
TGTCATTCTTTGTTGATCCT+TGG 0.335528 4.1:+25481015 None:intergenic
GAATAGTTGAAGCTTACTTT+TGG 0.351708 4.1:-25482149 MS.gene006772:CDS
GCTCTTGGAATAGTAAATTC+TGG 0.365262 4.1:-25481505 MS.gene006772:CDS
GTGCCAAAGCCTCTCCTTAT+GGG 0.366253 4.1:-25481101 MS.gene006772:CDS
TTTGTGCTTGATTACCTCAT+TGG 0.372285 4.1:+25481345 None:intergenic
CACTTGTCTTTAGGTTACTA+AGG 0.384876 4.1:-25483152 MS.gene006772:CDS
GCCATGTACTCTTCTATTGT+TGG 0.400220 4.1:+25481526 None:intergenic
TTTGGATGTTTATGAAGAAA+TGG 0.403778 4.1:-25481826 MS.gene006772:CDS
ATTGATTCAATCCAACGTTT+GGG 0.408878 4.1:-25483282 MS.gene006772:CDS
CTCAATACTCTGTTGGTAGA+AGG 0.414840 4.1:-25482104 MS.gene006772:CDS
CAAGAACTTGCCTAGGCTAC+TGG 0.415662 4.1:-25482452 MS.gene006772:CDS
GCATTACATTTCCACTTATG+AGG 0.423418 4.1:-25482425 MS.gene006772:CDS
GCCACACATATGAGGATTCA+TGG 0.435188 4.1:-25482592 MS.gene006772:CDS
TCAAATTAGCTTCCCTCAAA+GGG 0.439126 4.1:+25483304 None:intergenic
AACAACATCCTTCGTTGGAA+TGG 0.440069 4.1:-25481467 MS.gene006772:CDS
CCCTTTACTCATATTGGACA+TGG 0.445169 4.1:+25480972 None:intergenic
ATAGAAGAGTACATGGCTCT+TGG 0.456178 4.1:-25481520 MS.gene006772:CDS
ACTATTCTGTTGCGAGCAAA+AGG 0.456530 4.1:+25482165 None:intergenic
ACAACATCCTTCGTTGGAAT+GGG 0.463666 4.1:-25481466 MS.gene006772:CDS
ATTGATGTGCTTTATAAAGA+TGG 0.464933 4.1:-25481056 MS.gene006772:CDS
CATACTTTACTGAGGCTCAA+TGG 0.470064 4.1:-25481567 MS.gene006772:CDS
ACTCCCATAAGGAGAGGCTT+TGG 0.475163 4.1:+25481098 None:intergenic
AAAATGTCACAAACCCTTTG+AGG 0.477504 4.1:-25483317 MS.gene006772:CDS
AAACAACATCAGAAAGAAGT+TGG 0.478105 4.1:-25482340 MS.gene006772:CDS
TGTATGAAGCCACACATATG+AGG 0.482529 4.1:-25482600 MS.gene006772:CDS
ATCAAATTAGCTTCCCTCAA+AGG 0.485801 4.1:+25483303 None:intergenic
ATCTAAAATTGCTAGACTCA+TGG 0.490115 4.1:-25481380 MS.gene006772:CDS
GAGCCTCAGTAAAGTATGCC+TGG 0.491242 4.1:+25481572 None:intergenic
CAAGACAAGTCACAAATGCT+TGG 0.491285 4.1:-25481160 MS.gene006772:CDS
CAATGTCATGCTCAAAATGA+TGG 0.500528 4.1:+25483253 None:intergenic
AACTTCAAAGAAACACTTGT+TGG 0.503713 4.1:-25482646 MS.gene006772:CDS
TTCTTCATAAACATCCAAAA+CGG 0.505524 4.1:+25481830 None:intergenic
ATAGATGAAAAGATTGATCC+AGG 0.512165 4.1:-25481590 MS.gene006772:intron
AGAGGGATCTTCCTCATAAG+TGG 0.512911 4.1:+25482414 None:intergenic
TAATGCCTTGCCAGTAGCCT+AGG 0.520942 4.1:+25482442 None:intergenic
GTTGAGTGGTCATCATTTCA+AGG 0.522299 4.1:+25482527 None:intergenic
AAATGTCACAAACCCTTTGA+GGG 0.523712 4.1:-25483316 MS.gene006772:CDS
TTTGAGCCTCAATACTCTGT+TGG 0.527980 4.1:-25482111 MS.gene006772:CDS
TAGCAAAATTTCATGTATTG+TGG 0.530446 4.1:+25481858 None:intergenic
ATCATCAATTACTGAGGCCA+AGG 0.530914 4.1:+25482062 None:intergenic
AAGAAGTTCAAGAATGAGCA+AGG 0.540053 4.1:-25482670 MS.gene006772:CDS
ATCACAACAACATCCTTCGT+TGG 0.542322 4.1:-25481472 MS.gene006772:CDS
ACAAGTCACAAATGCTTGGA+AGG 0.546592 4.1:-25481156 MS.gene006772:CDS
AATGACCAAGGTGATATCCT+TGG 0.547258 4.1:-25482079 MS.gene006772:CDS
TTTGAGCAAGAGAGAGGGCA+TGG 0.551451 4.1:-25481251 MS.gene006772:CDS
AGACAAGTGCAATTGAATGA+AGG 0.552478 4.1:+25483166 None:intergenic
ACAAATTGAGAACTCCCATA+AGG 0.553139 4.1:+25481087 None:intergenic
TAGGTGGGATATAAGTTGCA+TGG 0.561499 4.1:-25481889 MS.gene006772:CDS
AGCAAAGTTGCATCATGAGA+GGG 0.572891 4.1:+25482397 None:intergenic
AATTAGTTTGAGCAAGAGAG+AGG 0.573171 4.1:-25481257 MS.gene006772:intron
GGATGATATTGTTTCCAATG+AGG 0.573519 4.1:-25481359 MS.gene006772:intron
AACACTTGTTGGTGATGTTG+AGG 0.576884 4.1:-25482635 MS.gene006772:CDS
ACACTTGTTGGTGATGTTGA+GGG 0.577847 4.1:-25482634 MS.gene006772:CDS
TGAAGAAATGGAGCAAGAGA+TGG 0.580004 4.1:-25481814 MS.gene006772:CDS
CACTCTTCAAGAACTTGCCT+AGG 0.581377 4.1:-25482459 MS.gene006772:CDS
ACCAACAATAGAAGAGTACA+TGG 0.590550 4.1:-25481527 MS.gene006772:CDS
TCCATGAATCCTCATATGTG+TGG 0.591257 4.1:+25482591 None:intergenic
ATTAGTTTGAGCAAGAGAGA+GGG 0.599571 4.1:-25481256 MS.gene006772:intron
CTTCTACCAACAGAGTATTG+AGG 0.601314 4.1:+25482105 None:intergenic
TAGCAAAGTTGCATCATGAG+AGG 0.612571 4.1:+25482396 None:intergenic
GGGATGCATTGCTACAGAAG+AGG 0.614970 4.1:-25481446 MS.gene006772:CDS
GAGCAAGAGATGGTCAAGGA+AGG 0.615004 4.1:-25481804 MS.gene006772:CDS
CTAGGCAAGTTCTTGAAGAG+TGG 0.622912 4.1:+25482460 None:intergenic
AATGCATCCCATTCCAACGA+AGG 0.632549 4.1:+25481459 None:intergenic
GATGGTATAATCCCAAACGT+TGG 0.633632 4.1:+25483271 None:intergenic
ACATATGAGGATTCATGGAG+AGG 0.637251 4.1:-25482587 MS.gene006772:CDS
TTTAGGTTACTAAGGCAACA+AGG 0.641189 4.1:-25483144 MS.gene006772:CDS
AAATTAATCATAGCTTAAAG+CGG 0.641455 4.1:-25482483 MS.gene006772:CDS
TTGAGAACTCCCATAAGGAG+AGG 0.643091 4.1:+25481092 None:intergenic
GGTCATCATTTCAAGGTGTG+AGG 0.643197 4.1:+25482534 None:intergenic
TGAGGCCAAGGATATCACCT+TGG 0.643385 4.1:+25482074 None:intergenic
GATTGCTATACTAACTCCCA+AGG 0.653661 4.1:-25481032 MS.gene006772:CDS
AATGGAGCAAGAGATGGTCA+AGG 0.660378 4.1:-25481808 MS.gene006772:CDS
ACTTGCCTAGGCTACTGGCA+AGG 0.666735 4.1:-25482447 MS.gene006772:CDS
CATGTAGCACTCAATAGCAG+AGG 0.671786 4.1:+25481226 None:intergenic
ATATATATCATCAATTACTG+AGG 0.671981 4.1:+25482056 None:intergenic
GATCCAGGCATACTTTACTG+AGG 0.675134 4.1:-25481575 MS.gene006772:CDS
AAGAAAAGGACTCAGTTGAG+TGG 0.683013 4.1:+25482513 None:intergenic
TGCTTGGAAGGATATCAATG+AGG 0.688978 4.1:-25481144 MS.gene006772:CDS
TGGTAGAAGGATAATGACCA+AGG 0.706697 4.1:-25482091 MS.gene006772:CDS
GAGTGCTACATGAATGAACA+TGG 0.715393 4.1:-25481215 MS.gene006772:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAATTAAAATGATTAAA+TGG - chr4.1:25481605-25481624 MS.gene006772:intron 10.0%
!! TTTAATTCAAATTAATTAAG+AGG + chr4.1:25482439-25482458 None:intergenic 10.0%
!!! AATATTGAACTATTTTTTTA+TGG - chr4.1:25482402-25482421 MS.gene006772:CDS 10.0%
!!! TGATTTTTTAAAATTATTAG+TGG - chr4.1:25481470-25481489 MS.gene006772:CDS 10.0%
!!! TTAATTTGAATTAAAATTCT+AGG - chr4.1:25482444-25482463 MS.gene006772:CDS 10.0%
!!! TTTTAAAAAATCACATAATT+CGG + chr4.1:25481463-25481482 None:intergenic 10.0%
!!! AAGAATTTTTACTTCATTTT+TGG + chr4.1:25482597-25482616 None:intergenic 15.0%
!!! TTTGTTTTCTTTAATGAATT+AGG - chr4.1:25482131-25482150 MS.gene006772:CDS 15.0%
!! AAATTAATCATAGCTTAAAG+CGG - chr4.1:25481869-25481888 MS.gene006772:CDS 20.0%
!! AAGCAAATTGAAATCTAATT+TGG + chr4.1:25481989-25482008 None:intergenic 20.0%
!! AATATTGAGATGTGAAAATT+TGG + chr4.1:25482388-25482407 None:intergenic 20.0%
!! ATATATATCATCAATTACTG+AGG + chr4.1:25482299-25482318 None:intergenic 20.0%
!! ATTTGAATTAAAATTCTAGG+TGG - chr4.1:25482447-25482466 MS.gene006772:CDS 20.0%
!! GATGATATATATGATGTTTA+TGG - chr4.1:25482307-25482326 MS.gene006772:intron 20.0%
!! TTTGAATTAAAATTCTAGGT+GGG - chr4.1:25482448-25482467 MS.gene006772:CDS 20.0%
!!! CATATTCTTGTAAATTGTTT+TGG - chr4.1:25482727-25482746 MS.gene006772:intron 20.0%
!!! TTAATTTTTGTAGCAAGAAA+AGG + chr4.1:25481856-25481875 None:intergenic 20.0%
! AAATCTAATACAAGTAGACA+AGG + chr4.1:25481261-25481280 None:intergenic 25.0%
! ATTGATGTGCTTTATAAAGA+TGG - chr4.1:25483296-25483315 MS.gene006772:CDS 25.0%
! CACAATAATCATGTTGAAAA+TGG - chr4.1:25481142-25481161 MS.gene006772:CDS 25.0%
! CTTAACCATATATTATCATG+TGG - chr4.1:25481644-25481663 MS.gene006772:intron 25.0%
! TAACATATATTGATCTCCAT+CGG - chr4.1:25481550-25481569 MS.gene006772:CDS 25.0%
! TAGCAAAATTTCATGTATTG+TGG + chr4.1:25482497-25482516 None:intergenic 25.0%
! TCAATATATGTTATGAAGCA+CGG + chr4.1:25481543-25481562 None:intergenic 25.0%
! TTCTTCATAAACATCCAAAA+CGG + chr4.1:25482525-25482544 None:intergenic 25.0%
! TTTGGATGTTTATGAAGAAA+TGG - chr4.1:25482526-25482545 MS.gene006772:CDS 25.0%
!! GTTTTCTTTAATGAATTAGG+TGG - chr4.1:25482134-25482153 MS.gene006772:CDS 25.0%
AAACAACATCAGAAAGAAGT+TGG - chr4.1:25482012-25482031 MS.gene006772:CDS 30.0%
AACTTCAAAGAAACACTTGT+TGG - chr4.1:25481706-25481725 MS.gene006772:intron 30.0%
ATAGATGAAAAGATTGATCC+AGG - chr4.1:25482762-25482781 MS.gene006772:intron 30.0%
ATTCAATTGCACTTGTCTTT+AGG - chr4.1:25481191-25481210 MS.gene006772:CDS 30.0%
GAGAACCACATGATAATATA+TGG + chr4.1:25481652-25481671 None:intergenic 30.0%
GTTAGTGTCTGTATCATATT+CGG - chr4.1:25481502-25481521 MS.gene006772:CDS 30.0%
TAGTGCTAAAAGAAAAAGTG+AGG - chr4.1:25480993-25481012 MS.gene006772:CDS 30.0%
TTCTTTAATGAATTAGGTGG+TGG - chr4.1:25482137-25482156 MS.gene006772:CDS 30.0%
! ATCTAAAATTGCTAGACTCA+TGG - chr4.1:25482972-25482991 MS.gene006772:intron 30.0%
! ATTGATTCAATCCAACGTTT+GGG - chr4.1:25481070-25481089 MS.gene006772:CDS 30.0%
! GAATAGTTGAAGCTTACTTT+TGG - chr4.1:25482203-25482222 MS.gene006772:intron 30.0%
! GGTTTGTGACATTTTTGTTA+GGG + chr4.1:25481030-25481049 None:intergenic 30.0%
! TTTTCACTCAAGCAATTGAA+AGG - chr4.1:25482350-25482369 MS.gene006772:CDS 30.0%
!! ATTTTGCTATAAAGCCGTTT+TGG - chr4.1:25482508-25482527 MS.gene006772:CDS 30.0%
!! GAAGCTTACTTTTGGATATT+GGG - chr4.1:25482211-25482230 MS.gene006772:intron 30.0%
!! TGAAGCTTACTTTTGGATAT+TGG - chr4.1:25482210-25482229 MS.gene006772:intron 30.0%
!!! AGATGAAGCATTGACAATTT+TGG + chr4.1:25482956-25482975 None:intergenic 30.0%
!!! ATGAAGCATTGACAATTTTG+GGG + chr4.1:25482954-25482973 None:intergenic 30.0%
!!! ATTGACAATTTTGGGGTTAT+TGG + chr4.1:25482947-25482966 None:intergenic 30.0%
!!! GATGAAGCATTGACAATTTT+GGG + chr4.1:25482955-25482974 None:intergenic 30.0%
AAAATGTCACAAACCCTTTG+AGG - chr4.1:25481035-25481054 MS.gene006772:CDS 35.0%
AAATGTCACAAACCCTTTGA+GGG - chr4.1:25481036-25481055 MS.gene006772:CDS 35.0%
AAGAAGTTCAAGAATGAGCA+AGG - chr4.1:25481682-25481701 MS.gene006772:intron 35.0%
ACAAATTGAGAACTCCCATA+AGG + chr4.1:25483268-25483287 None:intergenic 35.0%
AGACAAGTGCAATTGAATGA+AGG + chr4.1:25481189-25481208 None:intergenic 35.0%
CAATGTCATGCTCAAAATGA+TGG + chr4.1:25481102-25481121 None:intergenic 35.0%
CTAATACAAGTAGACAAGGT+GGG + chr4.1:25481257-25481276 None:intergenic 35.0%
GAAACCTCTTATTAAACCGA+TGG + chr4.1:25481569-25481588 None:intergenic 35.0%
GCATTACATTTCCACTTATG+AGG - chr4.1:25481927-25481946 MS.gene006772:intron 35.0%
GCTCTTGGAATAGTAAATTC+TGG - chr4.1:25482847-25482866 MS.gene006772:intron 35.0%
GGATGATATTGTTTCCAATG+AGG - chr4.1:25482993-25483012 MS.gene006772:intron 35.0%
GTCATTCTTTGTTGATCCTT+GGG + chr4.1:25483339-25483358 None:intergenic 35.0%
TAATACAAGTAGACAAGGTG+GGG + chr4.1:25481256-25481275 None:intergenic 35.0%
TCTAATACAAGTAGACAAGG+TGG + chr4.1:25481258-25481277 None:intergenic 35.0%
TGTCATTCTTTGTTGATCCT+TGG + chr4.1:25483340-25483359 None:intergenic 35.0%
TTTGTGCTTGATTACCTCAT+TGG + chr4.1:25483010-25483029 None:intergenic 35.0%
! AATTAGTTTGAGCAAGAGAG+AGG - chr4.1:25483095-25483114 MS.gene006772:intron 35.0%
! ACAAGGATATCACATTTTGC+CGG - chr4.1:25481225-25481244 MS.gene006772:CDS 35.0%
! ACCAACAATAGAAGAGTACA+TGG - chr4.1:25482825-25482844 MS.gene006772:intron 35.0%
! ATCAAATTAGCTTCCCTCAA+AGG + chr4.1:25481052-25481071 None:intergenic 35.0%
! ATTAGTTTGAGCAAGAGAGA+GGG - chr4.1:25483096-25483115 MS.gene006772:intron 35.0%
! CACTTGTCTTTAGGTTACTA+AGG - chr4.1:25481200-25481219 MS.gene006772:CDS 35.0%
! CATCATTTTGAGCATGACAT+TGG - chr4.1:25481100-25481119 MS.gene006772:CDS 35.0%
! GATTGATTCAATCCAACGTT+TGG - chr4.1:25481069-25481088 MS.gene006772:CDS 35.0%
! GGGTTTGTGACATTTTTGTT+AGG + chr4.1:25481031-25481050 None:intergenic 35.0%
! TACTCTTTTTAGATGTGTCC+TGG - chr4.1:25481385-25481404 MS.gene006772:CDS 35.0%
! TCAAATTAGCTTCCCTCAAA+GGG + chr4.1:25481051-25481070 None:intergenic 35.0%
! TTTAGGTTACTAAGGCAACA+AGG - chr4.1:25481208-25481227 MS.gene006772:CDS 35.0%
!! ATCTCCATCGGTTTAATAAG+AGG - chr4.1:25481562-25481581 MS.gene006772:CDS 35.0%
!! CTACAGAAGAGGTTTTTCAA+TGG - chr4.1:25482917-25482936 MS.gene006772:intron 35.0%
AACAACATCCTTCGTTGGAA+TGG - chr4.1:25482885-25482904 MS.gene006772:intron 40.0%
AAGAAAAGGACTCAGTTGAG+TGG + chr4.1:25481842-25481861 None:intergenic 40.0%
AATGACCAAGGTGATATCCT+TGG - chr4.1:25482273-25482292 MS.gene006772:intron 40.0%
ACAACATCCTTCGTTGGAAT+GGG - chr4.1:25482886-25482905 MS.gene006772:intron 40.0%
ACAAGTCACAAATGCTTGGA+AGG - chr4.1:25483196-25483215 MS.gene006772:CDS 40.0%
ACATATGAGGATTCATGGAG+AGG - chr4.1:25481765-25481784 MS.gene006772:intron 40.0%
ACTATTCTGTTGCGAGCAAA+AGG + chr4.1:25482190-25482209 None:intergenic 40.0%
AGCAAAGTTGCATCATGAGA+GGG + chr4.1:25481958-25481977 None:intergenic 40.0%
ATCACAACAACATCCTTCGT+TGG - chr4.1:25482880-25482899 MS.gene006772:intron 40.0%
ATCATCAATTACTGAGGCCA+AGG + chr4.1:25482293-25482312 None:intergenic 40.0%
CAAGACAAGTCACAAATGCT+TGG - chr4.1:25483192-25483211 MS.gene006772:CDS 40.0%
CATACTTTACTGAGGCTCAA+TGG - chr4.1:25482785-25482804 MS.gene006772:intron 40.0%
GAGTAGTGTTCGTGTTTCAT+AGG + chr4.1:25481370-25481389 None:intergenic 40.0%
GAGTGCTACATGAATGAACA+TGG - chr4.1:25483137-25483156 MS.gene006772:CDS 40.0%
GATGGTATAATCCCAAACGT+TGG + chr4.1:25481084-25481103 None:intergenic 40.0%
GATTGCTATACTAACTCCCA+AGG - chr4.1:25483320-25483339 MS.gene006772:CDS 40.0%
GCCATGTACTCTTCTATTGT+TGG + chr4.1:25482829-25482848 None:intergenic 40.0%
GTTGAGTGGTCATCATTTCA+AGG + chr4.1:25481828-25481847 None:intergenic 40.0%
TAGCAAAGTTGCATCATGAG+AGG + chr4.1:25481959-25481978 None:intergenic 40.0%
TAGGTGGGATATAAGTTGCA+TGG - chr4.1:25482463-25482482 MS.gene006772:CDS 40.0%
TCCATGAATCCTCATATGTG+TGG + chr4.1:25481764-25481783 None:intergenic 40.0%
TGAAGAAATGGAGCAAGAGA+TGG - chr4.1:25482538-25482557 MS.gene006772:CDS 40.0%
TGCTTGGAAGGATATCAATG+AGG - chr4.1:25483208-25483227 MS.gene006772:CDS 40.0%
TGGTAGAAGGATAATGACCA+AGG - chr4.1:25482261-25482280 MS.gene006772:intron 40.0%
TGTATGAAGCCACACATATG+AGG - chr4.1:25481752-25481771 MS.gene006772:intron 40.0%
TTTGAGCCTCAATACTCTGT+TGG - chr4.1:25482241-25482260 MS.gene006772:intron 40.0%
! ATAGAAGAGTACATGGCTCT+TGG - chr4.1:25482832-25482851 MS.gene006772:intron 40.0%
! CAAGGATATCACATTTTGCC+GGG - chr4.1:25481226-25481245 MS.gene006772:CDS 40.0%
! CTCAATACTCTGTTGGTAGA+AGG - chr4.1:25482248-25482267 MS.gene006772:intron 40.0%
!! AACACTTGTTGGTGATGTTG+AGG - chr4.1:25481717-25481736 MS.gene006772:intron 40.0%
!! ACACTTGTTGGTGATGTTGA+GGG - chr4.1:25481718-25481737 MS.gene006772:intron 40.0%
!! CTTCTACCAACAGAGTATTG+AGG + chr4.1:25482250-25482269 None:intergenic 40.0%
AATGCATCCCATTCCAACGA+AGG + chr4.1:25482896-25482915 None:intergenic 45.0%
AATGGAGCAAGAGATGGTCA+AGG - chr4.1:25482544-25482563 MS.gene006772:CDS 45.0%
AGAGGGATCTTCCTCATAAG+TGG + chr4.1:25481941-25481960 None:intergenic 45.0%
ATGAAGCACGGATACAGACA+TGG + chr4.1:25481531-25481550 None:intergenic 45.0%
CACTCTTCAAGAACTTGCCT+AGG - chr4.1:25481893-25481912 MS.gene006772:intron 45.0%
CATGTAGCACTCAATAGCAG+AGG + chr4.1:25483129-25483148 None:intergenic 45.0%
CTAGGCAAGTTCTTGAAGAG+TGG + chr4.1:25481895-25481914 None:intergenic 45.0%
GATCCAGGCATACTTTACTG+AGG - chr4.1:25482777-25482796 MS.gene006772:intron 45.0%
GCCACACATATGAGGATTCA+TGG - chr4.1:25481760-25481779 MS.gene006772:intron 45.0%
GGTCATCATTTCAAGGTGTG+AGG + chr4.1:25481821-25481840 None:intergenic 45.0%
TTGAGAACTCCCATAAGGAG+AGG + chr4.1:25483263-25483282 None:intergenic 45.0%
! AGAGGCTTTGGCACTTGAAT+TGG + chr4.1:25483245-25483264 None:intergenic 45.0%
! GTGTCTGAAACGTCTCGAAT+AGG + chr4.1:25482089-25482108 None:intergenic 45.0%
!! AAAATATAAATTTAAAAGAT+AGG + chr4.1:25483050-25483069 None:intergenic 5.0%
!! ATAAAAAAAATAAAAAAACA+AGG + chr4.1:25482633-25482652 None:intergenic 5.0%
ACTCCCATAAGGAGAGGCTT+TGG + chr4.1:25483257-25483276 None:intergenic 50.0%
AGTGCCAAAGCCTCTCCTTA+TGG - chr4.1:25483250-25483269 MS.gene006772:CDS 50.0%
ATCATGTGTCAGAGTCGCGT+CGG + chr4.1:25481436-25481455 None:intergenic 50.0%
CAAGAACTTGCCTAGGCTAC+TGG - chr4.1:25481900-25481919 MS.gene006772:intron 50.0%
GAGCAAGAGATGGTCAAGGA+AGG - chr4.1:25482548-25482567 MS.gene006772:CDS 50.0%
GAGCCTCAGTAAAGTATGCC+TGG + chr4.1:25482783-25482802 None:intergenic 50.0%
GGGATGCATTGCTACAGAAG+AGG - chr4.1:25482906-25482925 MS.gene006772:intron 50.0%
GTGCCAAAGCCTCTCCTTAT+GGG - chr4.1:25483251-25483270 MS.gene006772:CDS 50.0%
TAATGCCTTGCCAGTAGCCT+AGG + chr4.1:25481913-25481932 None:intergenic 50.0%
TAGACAAGGTGGGGTTTACC+CGG + chr4.1:25481247-25481266 None:intergenic 50.0%
TGAGGCCAAGGATATCACCT+TGG + chr4.1:25482281-25482300 None:intergenic 50.0%
TTTGAGCAAGAGAGAGGGCA+TGG - chr4.1:25483101-25483120 MS.gene006772:intron 50.0%
AACGACACGTGTTCGACACC+AGG + chr4.1:25481406-25481425 None:intergenic 55.0%
ACTTGCCTAGGCTACTGGCA+AGG - chr4.1:25481905-25481924 MS.gene006772:intron 55.0%
! TGTCAGAGTCGCGTCGGTGT+TGG + chr4.1:25481430-25481449 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 25480980 25483394 25480980 ID=MS.gene006772
chr4.1 mRNA 25480980 25483394 25480980 ID=MS.gene006772.t1;Parent=MS.gene006772
chr4.1 exon 25483127 25483394 25483127 ID=MS.gene006772.t1.exon1;Parent=MS.gene006772.t1
chr4.1 CDS 25483127 25483394 25483127 ID=cds.MS.gene006772.t1;Parent=MS.gene006772.t1
chr4.1 exon 25482328 25482700 25482328 ID=MS.gene006772.t1.exon2;Parent=MS.gene006772.t1
chr4.1 CDS 25482328 25482700 25482328 ID=cds.MS.gene006772.t1;Parent=MS.gene006772.t1
chr4.1 exon 25482003 25482221 25482003 ID=MS.gene006772.t1.exon3;Parent=MS.gene006772.t1
chr4.1 CDS 25482003 25482221 25482003 ID=cds.MS.gene006772.t1;Parent=MS.gene006772.t1
chr4.1 exon 25481770 25481908 25481770 ID=MS.gene006772.t1.exon4;Parent=MS.gene006772.t1
chr4.1 CDS 25481770 25481908 25481770 ID=cds.MS.gene006772.t1;Parent=MS.gene006772.t1
chr4.1 exon 25481360 25481608 25481360 ID=MS.gene006772.t1.exon5;Parent=MS.gene006772.t1
chr4.1 CDS 25481360 25481608 25481360 ID=cds.MS.gene006772.t1;Parent=MS.gene006772.t1
chr4.1 exon 25480980 25481273 25480980 ID=MS.gene006772.t1.exon6;Parent=MS.gene006772.t1
chr4.1 CDS 25480980 25481273 25480980 ID=cds.MS.gene006772.t1;Parent=MS.gene006772.t1
Gene Sequence

>MS.gene006772

ATGAAGCAGATTATAGTGCTAAAAGAAAAAGTGAGGCAGATGCTAGTCCCTAACAAAAATGTCACAAACCCTTTGAGGGAAGCTAATTTGATTGATTCAATCCAACGTTTGGGATTATACCATCATTTTGAGCATGACATTGGAGAATTATTGCGACATATTCACAATAATCATGTTGAAAATGGAACAATAACTCTTAACGAAGACCTTCATTCAATTGCACTTGTCTTTAGGTTACTAAGGCAACAAGGATATCACATTTTGCCGGGTAAACCCCACCTTGTCTACTTGTATTAGATTTTAGTCTTATTTTACGTAAATGTATACAACACATTAAACATCTAAATTCAAAGTGCTTAAAGCGTGCTCAGATGAGATGACATATATCCTATGAAACACGAACACTACTCTTTTTAGATGTGTCCTGGTGTCGAACACGTGTCGTTTCCAACACCGACGCGACTCTGACACATGATTACACCGAATTATGTGATTTTTTAAAATTATTAGTGGTGTCAGTGTGTTAGTGTCTGTATCATATTCGGTGTCCATGTCTGTATCCGTGCTTCATAACATATATTGATCTCCATCGGTTTAATAAGAGGTTTCAAGTCACGTGAACTTAACAAAATTAAAATGATTAAATGGTTTGAAGAACATAAAACTTAACCATATATTATCATGTGGTTCTCAGATGCTTTTAAGAAGTTCAAGAATGAGCAAGGAAACTTCAAAGAAACACTTGTTGGTGATGTTGAGGGAATGCTAAGTTTGTATGAAGCCACACATATGAGGATTCATGGAGAGGATATATTAGATGAAGCACTTTCTTTCACTTCCTCACACCTTGAAATGATGACCACTCAACTGAGTCCTTTTCTTGCTACAAAAATTAATCATAGCTTAAAGCGGCCACTCTTCAAGAACTTGCCTAGGCTACTGGCAAGGCATTACATTTCCACTTATGAGGAAGATCCCTCTCATGATGCAACTTTGCTATTACTTGCCAAATTAGATTTCAATTTGCTTCAAAAACAACATCAGAAAGAAGTTGGCGATATTTCAAAGTAAGTTTTTTTTTTTTTTTTTATTCTTTTAAAGTTAATCCTATTCGAGACGTTTCAGACACTAAATATGACTATCAAACTAAGTTTGTTTTCTTTAATGAATTAGGTGGTGGAAAGATTTAGATTTTGCAACAAAACTACCTTTTGCTCGCAACAGAATAGTTGAAGCTTACTTTTGGATATTGGGAGTGTATTTTGAGCCTCAATACTCTGTTGGTAGAAGGATAATGACCAAGGTGATATCCTTGGCCTCAGTAATTGATGATATATATGATGTTTATGGTACAATAGAAGAGCTACAACTTTTCACTCAAGCAATTGAAAGGTTTGAATCTCTACCAAATTTTCACATCTCAATATTGAACTATTTTTTTATGGAAATTTATTAACCTCTTAATTAATTTGAATTAAAATTCTAGGTGGGATATAAGTTGCATGGATTTTCTACCACAATACATGAAATTTTGCTATAAAGCCGTTTTGGATGTTTATGAAGAAATGGAGCAAGAGATGGTCAAGGAAGGCAGAGCATTTTGTGTAACATATGCCAAAAATGAAGTAAAAATTCTTACTCTTCATTTTTCCTTGTTTTTTTATTTTTTTTATTTTAAAAGAGATTAAAATGAGAGAATATTTTTTCTTGCAAGATTAAAATGAGAAAATGTGCTTCATGTCTAACTCATATTCTTGTAAATTGTTTTGGTTCATGTGCACAATAGATGAAAAGATTGATCCAGGCATACTTTACTGAGGCTCAATGGTTCAGTAGAAAATATATACCAACAATAGAAGAGTACATGGCTCTTGGAATAGTAAATTCTGGTTACTATCTAATCACAACAACATCCTTCGTTGGAATGGGATGCATTGCTACAGAAGAGGTTTTTCAATGGTTAACCAATAACCCCAAAATTGTCAATGCTTCATCTAAAATTGCTAGACTCATGGATGATATTGTTTCCAATGAGGTAATCAAGCACAAAAATTAATTTATCAATTTCCTATCTTTTAAATTTATATTTTAAATAAATATGTTTTTTTTTATATTAATTAGTTTGAGCAAGAGAGAGGGCATGGTGCCTCTGCTATTGAGTGCTACATGAATGAACATGGTGTGTCTAGAGAAGATGCAGTTAATGAACTTTCAAGACAAGTCACAAATGCTTGGAAGGATATCAATGAGGAACTTCTTGATCCAATTCAAGTGCCAAAGCCTCTCCTTATGGGAGTTCTCAATTTGTCACGTGTCATTGATGTGCTTTATAAAGATGGAGATTGCTATACTAACTCCCAAGGATCAACAAAGAATGACATAATATCTATCTTGTTGAATCCATGTCCAATATGA
Protein sequence

>MS.gene006772.t1

MKQIIVLKEKVRQMLVPNKNVTNPLREANLIDSIQRLGLYHHFEHDIGELLRHIHNNHVENGTITLNEDLHSIALVFRLLRQQGYHILPDAFKKFKNEQGNFKETLVGDVEGMLSLYEATHMRIHGEDILDEALSFTSSHLEMMTTQLSPFLATKINHSLKRPLFKNLPRLLARHYISTYEEDPSHDATLLLLAKLDFNLLQKQHQKEVGDISKWWKDLDFATKLPFARNRIVEAYFWILGVYFEPQYSVGRRIMTKVISLASVIDDIYDVYGTIEELQLFTQAIERWDISCMDFLPQYMKFCYKAVLDVYEEMEQEMVKEGRAFCVTYAKNEMKRLIQAYFTEAQWFSRKYIPTIEEYMALGIVNSGYYLITTTSFVGMGCIATEEVFQWLTNNPKIVNASSKIARLMDDIVSNEFEQERGHGASAIECYMNEHGVSREDAVNELSRQVTNAWKDINEELLDPIQVPKPLLMGVLNLSRVIDVLYKDGDCYTNSQGSTKNDIISILLNPCPI