AlfalfaGEDB | MS.gene045736

M. sativa cultivar XinJiangDaYe / MS.gene045736

KEGG: -
GO: GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0010333 GO:0016829 GO:0016835 GO:0016838 GO:0043167 GO:0043169 GO:0046872
PFAM: PF01397.24 PF03936.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045736.t1	XP_013464201.1	82.1	591	71	3	1	556	1	591	6.20E-282	979.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045736.t1	Q93X23	57.8	531	216	4	28	551	65	594	3.1e-173	609.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045736.t1	A0A072V9D0	82.1	591	71	3	1	556	1	591	4.5e-282	979.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene068447	MS.gene045736	PPI
MS.gene042766	MS.gene045736	PPI
MS.gene068440	MS.gene045736	PPI
MS.gene54235	MS.gene045736	PPI
MS.gene54248	MS.gene045736	PPI
MS.gene42723	MS.gene045736	PPI
MS.gene54270	MS.gene045736	PPI
MS.gene042753	MS.gene045736	PPI
MS.gene062697	MS.gene045736	PPI
MS.gene062687	MS.gene045736	PPI
MS.gene90432	MS.gene045736	PPI
MS.gene066316	MS.gene045736	PPI
MS.gene045736	MS.gene42723	PPI
MS.gene032057	MS.gene045736	PPI
MS.gene062668	MS.gene045736	PPI
MS.gene37786	MS.gene045736	PPI
MS.gene89517	MS.gene045736	PPI
MS.gene56552	MS.gene045736	PPI
MS.gene062690	MS.gene045736	PPI
MS.gene08035	MS.gene045736	PPI
MS.gene062696	MS.gene045736	PPI
MS.gene77594	MS.gene045736	PPI
MS.gene006772	MS.gene045736	PPI
MS.gene062678	MS.gene045736	PPI
MS.gene062695	MS.gene045736	PPI
MS.gene062667	MS.gene045736	PPI
MS.gene038985	MS.gene045736	PPI
MS.gene54267	MS.gene045736	PPI
MS.gene009763	MS.gene045736	PPI
MS.gene068431	MS.gene045736	PPI
MS.gene068442	MS.gene045736	PPI
MS.gene042759	MS.gene045736	PPI
MS.gene54268	MS.gene045736	PPI
MS.gene35872	MS.gene045736	PPI
MS.gene54260	MS.gene045736	PPI
MS.gene042761	MS.gene045736	PPI
MS.gene062686	MS.gene045736	PPI
MS.gene54255	MS.gene045736	PPI
MS.gene068436	MS.gene045736	PPI
MS.gene041989	MS.gene045736	PPI
MS.gene54250	MS.gene045736	PPI
MS.gene069389	MS.gene045736	PPI
MS.gene88992	MS.gene045736	PPI
MS.gene54232	MS.gene045736	PPI
MS.gene066310	MS.gene045736	PPI
MS.gene062682	MS.gene045736	PPI
MS.gene042752	MS.gene045736	PPI
MS.gene042750	MS.gene045736	PPI
MS.gene54261	MS.gene045736	PPI
MS.gene062802	MS.gene045736	PPI
MS.gene042769	MS.gene045736	PPI
MS.gene035927	MS.gene045736	PPI
MS.gene069726	MS.gene045736	PPI
MS.gene042758	MS.gene045736	PPI
MS.gene54263	MS.gene045736	PPI
MS.gene031524	MS.gene045736	PPI
MS.gene068432	MS.gene045736	PPI
MS.gene010250	MS.gene045736	PPI
MS.gene048078	MS.gene045736	PPI
MS.gene062683	MS.gene045736	PPI
MS.gene96807	MS.gene045736	PPI
MS.gene038982	MS.gene045736	PPI
MS.gene54264	MS.gene045736	PPI
MS.gene042760	MS.gene045736	PPI
MS.gene54262	MS.gene045736	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045736.t1	MTR_2g064425	82.064	591	71	3	1	556	1	591	0.0	990
MS.gene045736.t1	MTR_2g064425	79.865	591	69	4	1	556	1	576	0.0	952
MS.gene045736.t1	MTR_2g065450	65.874	589	162	4	1	550	1	589	0.0	764
MS.gene045736.t1	MTR_8g057020	69.011	526	160	2	28	550	63	588	0.0	763
MS.gene045736.t1	MTR_2g064425	78.624	407	52	3	1	372	1	407	0.0	646
MS.gene045736.t1	MTR_2g064425	75.430	407	50	4	1	372	1	392	0.0	607
MS.gene045736.t1	MTR_4g045810	46.893	531	267	8	29	549	54	579	5.70e-156	458
MS.gene045736.t1	MTR_7g010710	38.318	535	307	10	29	548	26	552	4.71e-121	368
MS.gene045736.t1	MTR_4g081460	36.412	563	332	8	11	551	4	562	1.35e-114	352
MS.gene045736.t1	MTR_2g089120	37.870	507	307	5	46	550	64	564	3.59e-111	343
MS.gene045736.t1	MTR_2g089130	37.945	506	307	4	46	550	64	563	3.57e-109	338
MS.gene045736.t1	MTR_6g039440	34.638	511	325	6	48	551	45	553	5.51e-105	326
MS.gene045736.t1	MTR_6g008560	35.409	514	322	6	46	551	46	557	2.20e-104	325
MS.gene045736.t1	MTR_4g048460	35.503	507	318	6	52	551	57	561	3.65e-103	322
MS.gene045736.t1	MTR_5g062230	35.098	510	324	4	48	551	45	553	2.75e-101	317
MS.gene045736.t1	MTR_5g094620	34.263	502	312	4	52	551	52	537	2.92e-99	311
MS.gene045736.t1	MTR_5g073200	35.140	535	334	8	28	551	17	549	7.12e-98	308
MS.gene045736.t1	MTR_3g464190	34.051	511	329	4	48	551	48	557	3.40e-97	306
MS.gene045736.t1	MTR_8g007515	34.444	540	334	11	28	551	21	556	3.28e-95	301
MS.gene045736.t1	MTR_2g081980	34.175	515	326	7	48	551	45	557	7.32e-95	300
MS.gene045736.t1	MTR_2g082010	34.690	516	322	9	47	551	57	568	5.92e-94	298
MS.gene045736.t1	MTR_5g073260	33.333	513	331	6	48	551	42	552	1.32e-89	286
MS.gene045736.t1	MTR_4g048460	38.177	406	243	5	52	451	57	460	2.55e-88	280
MS.gene045736.t1	MTR_2g082060	33.269	517	319	9	47	551	51	553	6.13e-84	272
MS.gene045736.t1	MTR_4g092020	33.789	512	325	7	45	549	65	569	1.20e-82	269
MS.gene045736.t1	MTR_4g092010	33.202	506	322	8	36	531	43	542	3.35e-82	268
MS.gene045736.t1	MTR_2g010960	33.205	518	330	7	42	545	2	517	5.73e-82	265
MS.gene045736.t1	MTR_3g465090	32.869	502	310	11	42	525	2	494	1.69e-80	261
MS.gene045736.t1	MTR_6g064980	32.239	518	330	11	42	545	2	512	1.16e-79	259
MS.gene045736.t1	MTR_3g063170	31.717	495	325	8	42	525	2	494	1.66e-78	256
MS.gene045736.t1	MTR_3g052120	32.186	494	324	6	42	525	2	494	6.70e-78	254
MS.gene045736.t1	MTR_3g063140	32.008	503	313	11	42	525	2	494	2.17e-77	253
MS.gene045736.t1	MTR_6g008560	33.747	403	263	4	151	551	1	401	5.18e-77	249
MS.gene045736.t1	MTR_3g063120	32.636	478	295	11	67	525	1	470	4.60e-76	249
MS.gene045736.t1	MTR_4g019550	31.107	524	325	8	47	551	52	558	6.31e-75	248
MS.gene045736.t1	MTR_6g065040	33.418	392	254	6	140	525	2	392	1.01e-65	219
MS.gene045736.t1	MTR_2g082050	30.315	508	296	9	47	549	3	457	2.53e-64	217
MS.gene045736.t1	MTR_3g058160	30.155	451	278	14	69	489	65	508	1.15e-45	169
MS.gene045736.t1	MTR_3g058160	30.155	451	278	14	69	489	256	699	5.69e-45	170
MS.gene045736.t1	MTR_3g058160	30.155	451	278	14	69	489	269	712	5.96e-45	170
MS.gene045736.t1	MTR_3g058160	30.155	451	278	14	69	489	276	719	6.68e-45	170
MS.gene045736.t1	MTR_2g064295	29.314	423	265	11	69	463	256	672	2.72e-44	168
MS.gene045736.t1	MTR_2g012870	29.927	411	260	9	76	463	261	666	1.31e-43	166
MS.gene045736.t1	MTR_3g058160	28.814	472	278	14	69	489	276	740	3.37e-42	162
MS.gene045736.t1	MTR_2g012900	30.979	439	257	13	64	463	249	680	2.10e-39	154
MS.gene045736.t1	MTR_3g063160	30.717	293	187	5	42	324	2	288	3.35e-37	139
MS.gene045736.t1	MTR_7g050990	43.750	144	72	1	197	340	29	163	3.68e-31	129
MS.gene045736.t1	MTR_5g094530	33.175	211	103	5	165	372	5	180	2.81e-25	103
MS.gene045736.t1	MTR_7g094970	26.923	286	172	7	73	328	212	490	1.48e-22	102
MS.gene045736.t1	MTR_7g011663	29.615	260	152	6	72	301	349	607	3.83e-22	101
MS.gene045736.t1	MTR_7g011770	28.571	287	173	7	73	328	246	531	1.91e-21	99.0
MS.gene045736.t1	MTR_5g030050	26.562	256	160	6	73	305	4	254	4.29e-20	91.7
MS.gene045736.t1	MTR_5g081730	51.515	99	43	2	157	253	16	111	1.59e-19	85.9
MS.gene045736.t1	MTR_7g057340	22.674	172	132	1	381	551	1	172	2.24e-13	68.9
MS.gene045736.t1	MTR_7g057390	22.093	172	130	2	381	551	1	169	2.99e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045736.t1	AT3G25830	48.263	518	248	10	51	551	80	594	6.81e-166	484
MS.gene045736.t1	AT3G25820	48.263	518	248	10	51	551	80	594	6.81e-166	484
MS.gene045736.t1	AT4G16730	45.143	525	275	5	40	551	15	539	2.52e-162	473
MS.gene045736.t1	AT2G24210	47.308	520	257	11	46	551	74	590	1.13e-159	468
MS.gene045736.t1	AT3G25810	47.441	508	246	10	62	551	93	597	8.80e-158	464
MS.gene045736.t1	AT4G16740	45.489	521	251	9	50	551	58	564	2.53e-154	454
MS.gene045736.t1	AT4G16730	45.528	492	255	5	73	551	2	493	5.97e-154	450
MS.gene045736.t1	AT3G25820	47.619	357	173	7	51	396	80	433	7.33e-110	335
MS.gene045736.t1	AT4G16740	46.334	341	154	6	50	372	58	387	6.55e-96	298
MS.gene045736.t1	AT1G61680	35.118	467	295	5	68	528	92	556	7.18e-88	282
MS.gene045736.t1	AT5G23960	32.900	538	338	10	29	551	16	545	7.54e-88	281
MS.gene045736.t1	AT5G23960	33.148	540	336	11	29	551	16	547	4.25e-87	280
MS.gene045736.t1	AT1G61680	34.904	467	289	6	68	528	92	549	1.12e-86	279
MS.gene045736.t1	AT3G25820	48.780	287	133	7	51	326	80	363	3.43e-84	267
MS.gene045736.t1	AT3G25820	48.780	287	133	7	51	326	80	363	3.43e-84	267
MS.gene045736.t1	AT4G16740	45.253	316	144	6	50	347	58	362	1.70e-78	252
MS.gene045736.t1	AT4G16740	45.253	316	144	6	50	347	58	362	1.70e-78	252
MS.gene045736.t1	AT1G70080	32.645	484	314	6	72	544	122	604	1.59e-72	243
MS.gene045736.t1	AT3G14520	28.656	506	344	5	58	551	104	604	3.69e-71	239
MS.gene045736.t1	AT1G31950	28.205	507	343	8	59	551	108	607	1.52e-70	238
MS.gene045736.t1	AT1G31950	28.205	507	343	8	59	551	107	606	1.65e-70	238
MS.gene045736.t1	AT3G29410	27.495	531	373	5	26	551	80	603	1.90e-68	232
MS.gene045736.t1	AT3G29410	27.309	498	358	3	58	551	1	498	2.82e-67	226
MS.gene045736.t1	AT3G14540	27.930	512	340	8	58	551	101	601	9.96e-67	228
MS.gene045736.t1	AT1G33750	28.571	511	347	7	52	551	100	603	8.64e-66	225
MS.gene045736.t1	AT3G32030	27.308	509	363	6	50	551	96	604	7.96e-62	214
MS.gene045736.t1	AT3G14490	29.502	522	331	14	52	551	95	601	7.73e-61	211
MS.gene045736.t1	AT4G15870	27.833	503	341	8	52	544	101	591	7.00e-58	203
MS.gene045736.t1	AT3G29110	28.440	545	369	10	17	549	60	595	1.43e-56	200
MS.gene045736.t1	AT3G29190	29.077	509	352	6	52	551	93	601	1.49e-56	200
MS.gene045736.t1	AT1G31950	28.676	408	274	6	59	455	108	509	3.48e-56	197
MS.gene045736.t1	AT5G44630	28.926	484	336	7	73	549	73	555	1.65e-54	193
MS.gene045736.t1	AT3G14540	27.051	451	301	8	28	460	68	508	4.70e-53	188
MS.gene045736.t1	AT4G20200	28.221	489	337	7	73	551	120	604	1.86e-51	186
MS.gene045736.t1	AT4G20230	26.839	503	358	7	52	544	108	610	7.82e-51	184
MS.gene045736.t1	AT4G20230	26.839	503	358	7	52	544	96	598	8.35e-51	184
MS.gene045736.t1	AT4G20210	26.501	483	349	5	75	551	118	600	5.69e-50	182
MS.gene045736.t1	AT1G66020	28.046	517	347	11	52	551	90	598	1.14e-48	178
MS.gene045736.t1	AT4G13300	26.265	514	368	9	46	549	40	552	2.99e-48	176
MS.gene045736.t1	AT1G48800	27.734	512	352	9	47	544	92	599	1.14e-47	176
MS.gene045736.t1	AT1G48820	28.458	506	318	10	47	546	92	559	1.68e-47	174
MS.gene045736.t1	AT2G23230	26.511	513	364	7	44	544	82	593	1.28e-45	170
MS.gene045736.t1	AT2G23230	26.499	517	360	8	44	547	82	591	2.50e-45	169
MS.gene045736.t1	AT4G13280	26.151	478	343	8	82	551	79	554	1.82e-44	166
MS.gene045736.t1	AT1G79460	26.901	513	338	12	69	551	274	779	9.12e-43	164
MS.gene045736.t1	AT5G48110	28.947	342	237	6	212	551	2	339	3.30e-41	152
MS.gene045736.t1	AT4G13280	25.314	478	317	10	82	551	79	524	7.99e-37	144
MS.gene045736.t1	AT1G66020	30.915	317	206	7	52	360	90	401	1.90e-36	141
MS.gene045736.t1	AT1G61120	30.227	440	271	14	66	480	282	710	5.63e-35	140
MS.gene045736.t1	AT4G02780	30.798	263	149	6	73	304	335	595	2.08e-22	102

Find 87 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTACTTGAATTTGCTAAAT+TGG	0.149491	2.2:+25272454	MS.gene045736:CDS
CCCTAATTTCATTATTAAAA+TGG	0.155653	2.2:-25271843	None:intergenic
GCAATTTGATTCTGAATTTC+AGG	0.181621	2.2:-25274217	None:intergenic
ATTTGAAGATGAACCTATAT+TGG	0.220192	2.2:+25272253	MS.gene045736:CDS
ATACGTTCTTATCTTCATTT+TGG	0.245554	2.2:-25272322	None:intergenic
ATCATCAATGATGGTAATTA+AGG	0.253672	2.2:-25273134	None:intergenic
GATTCCACATTCATTCAAAA+TGG	0.278555	2.2:+25273781	MS.gene045736:CDS
TAATGAAATTAGGGACATAT+TGG	0.284373	2.2:+25271852	MS.gene045736:CDS
TTGAGTTGTGCGATGGAAAT+TGG	0.303929	2.2:-25270474	None:intergenic
TTTCGTAAATATTTAAAATC+TGG	0.312383	2.2:-25273091	None:intergenic
GATTCTGAATTTCAGGATCT+TGG	0.336257	2.2:-25274210	None:intergenic
CAATGCTACATGAATGAAAC+TGG	0.341142	2.2:+25274016	MS.gene045736:CDS
ATGTATACTTAAGGTCTTCT+TGG	0.346794	2.2:-25272502	None:intergenic
TCTCTTGCTTCATCCAATAT+AGG	0.348986	2.2:-25272266	None:intergenic
CTAATGGCTTTACCTATGAT+TGG	0.363161	2.2:+25270377	None:intergenic
GATTGGTTGAGAACTTTGTT+TGG	0.376926	2.2:+25273047	MS.gene045736:CDS
ACTTCAAATAAGTCATGCTT+TGG	0.381812	2.2:+25272346	MS.gene045736:CDS
TTTGGAACATCCACTGCATT+GGG	0.391831	2.2:+25272364	MS.gene045736:CDS
ATGCTTTGCAAGGGATAGAT+TGG	0.394984	2.2:+25273030	MS.gene045736:CDS
TAAGCATGAGTAAGTATAGT+AGG	0.415253	2.2:-25273752	None:intergenic
GAAATTTGTAATTTCAAGAA+AGG	0.419193	2.2:+25272176	MS.gene045736:CDS
CTAGAAAGAGTTCCAATCAT+AGG	0.420371	2.2:-25270389	None:intergenic
TTTGTAATTTCAAGAAAGGT+AGG	0.421777	2.2:+25272180	MS.gene045736:CDS
GATTGGAACTCTTTCTAGCT+TGG	0.425228	2.2:+25270394	MS.gene045736:CDS
GCTTCCTCCTGTTGAAAAGA+AGG	0.427249	2.2:+25270427	MS.gene045736:CDS
TCTTCACAAAAGCAGTTGAT+AGG	0.429846	2.2:+25273194	MS.gene045736:CDS
TTTCAGGATCTTGGTTGGTA+TGG	0.431471	2.2:-25274201	None:intergenic
AGCACCTTGATGTATACTTA+AGG	0.432930	2.2:-25272511	None:intergenic
GGTAGGTTTGTTGACGTTGA+GGG	0.440364	2.2:+25272197	MS.gene045736:CDS
ATGTAGCATTGAATTGACTT+TGG	0.445177	2.2:-25274004	None:intergenic
TGCTGAAAGTCTAATTATGT+TGG	0.450285	2.2:-25273831	None:intergenic
CTTTGGAACATCCACTGCAT+TGG	0.453996	2.2:+25272363	MS.gene045736:CDS
TGCGGTAAATCTTGCTAGAA+TGG	0.458860	2.2:+25274150	MS.gene045736:CDS
GAGTCCCTCGATGGGAGGCT+AGG	0.467693	2.2:+25272387	MS.gene045736:CDS
AGGGTTCTACTGTAGCAATA+TGG	0.469057	2.2:+25270447	MS.gene045736:CDS
AAGTATAGTAGGTGCACCTA+TGG	0.470676	2.2:-25273741	None:intergenic
TTTGTACATACAATAGGAAT+CGG	0.476951	2.2:-25274256	None:intergenic
GGCTTGGTGTGTATCCACTA+TGG	0.480255	2.2:-25273688	None:intergenic
CTGAATTTCAGGATCTTGGT+TGG	0.483854	2.2:-25274206	None:intergenic
ATTGATGTATTGCAAAGGCT+TGG	0.488943	2.2:+25271811	MS.gene045736:CDS
TCTATGTATTCTCCAAGGCT+TGG	0.489111	2.2:-25273704	None:intergenic
AAGAATGATGCTTAACAAAA+CGG	0.497193	2.2:+25271762	MS.gene045736:CDS
TTCATTCAAAATGGAAGACT+TGG	0.497580	2.2:+25273790	MS.gene045736:CDS
GAGACAATTTATGCTTTGCA+AGG	0.504853	2.2:+25273020	MS.gene045736:CDS
TGCATTGGGGAGTCCCTCGA+TGG	0.509350	2.2:+25272378	MS.gene045736:CDS
CTTCCTCCTGTTGAAAAGAA+GGG	0.519768	2.2:+25270428	MS.gene045736:CDS
AGGTAGGTTTGTTGACGTTG+AGG	0.522510	2.2:+25272196	MS.gene045736:CDS
TTTATGTATCATCGTGGAGA+TGG	0.524795	2.2:+25274178	MS.gene045736:CDS
GAGAACTTTGTTTGGACTGT+GGG	0.531644	2.2:+25273055	MS.gene045736:CDS
AGTGGATACACACCAAGCCT+TGG	0.533055	2.2:+25273692	MS.gene045736:CDS
CATATTTGATTGAAGCAAAG+TGG	0.534124	2.2:+25273663	MS.gene045736:CDS
CATGGTTATGATATATCACC+AGG	0.538113	2.2:+25271955	MS.gene045736:CDS
GAGAATACATAGAGAATGCA+TGG	0.541704	2.2:+25273714	MS.gene045736:CDS
AACTGGAGCTTCTGAAGTTG+AGG	0.542366	2.2:+25274033	MS.gene045736:CDS
CTTCCACTCTATAAACATCA+TGG	0.551044	2.2:-25274074	None:intergenic
TGGTGGAAAAGAACTAAACT+TGG	0.552083	2.2:+25272998	MS.gene045736:CDS
ATCGTACACATCATCAATGA+TGG	0.554157	2.2:-25273143	None:intergenic
TTACGCCTTGCTAATGACCT+CGG	0.563093	2.2:+25273860	MS.gene045736:CDS
GATGATGTGTACGATGTGTA+TGG	0.566503	2.2:+25273151	MS.gene045736:CDS
TTCAGACTCTTGAGGCAACA+TGG	0.576372	2.2:+25271937	MS.gene045736:CDS
AGTCCATGATGTTTATAGAG+TGG	0.576983	2.2:+25274071	MS.gene045736:CDS
AGACAATTTATGCTTTGCAA+GGG	0.578399	2.2:+25273021	MS.gene045736:CDS
CATTCTCTATGTATTCTCCA+AGG	0.581762	2.2:-25273709	None:intergenic
TAGATGGGATCTAAATGCCA+TGG	0.583752	2.2:+25273314	MS.gene045736:intron
GAGAATGCATGGATATCCAT+AGG	0.583758	2.2:+25273725	MS.gene045736:CDS
GTAGTTTGGGAGAGAATCCA+TGG	0.590044	2.2:-25273331	None:intergenic
AACTTACTTTATATGTTCCG+AGG	0.590775	2.2:-25273877	None:intergenic
CCTTGCATTGAGTTGTGCGA+TGG	0.594872	2.2:-25270481	None:intergenic
ACCACCTAGCCTCCCATCGA+GGG	0.602909	2.2:-25272391	None:intergenic
AGTTTATTGATGTATTGCAA+AGG	0.603533	2.2:+25271806	MS.gene045736:CDS
GCATTGGGGAGTCCCTCGAT+GGG	0.605686	2.2:+25272379	MS.gene045736:CDS
AAGTAATGTACACAAAGCAA+TGG	0.607124	2.2:+25271719	MS.gene045736:CDS
CGAGGGACTCCCCAATGCAG+TGG	0.607874	2.2:-25272374	None:intergenic
ATGTTCCGAGGTCATTAGCA+AGG	0.610811	2.2:-25273865	None:intergenic
CCATCGCACAACTCAATGCA+AGG	0.612454	2.2:+25270481	MS.gene045736:CDS
TTTACGAAAAGTTATGACAA+AGG	0.613165	2.2:+25273105	MS.gene045736:CDS
GAAGCAAAGTGGTACCATAG+TGG	0.615723	2.2:+25273674	MS.gene045736:CDS
TTGGGGAGTCCCTCGATGGG+AGG	0.619853	2.2:+25272382	MS.gene045736:CDS
AAGACCTTAAGTATACATCA+AGG	0.621822	2.2:+25272507	MS.gene045736:CDS
TCCCTCGATGGGAGGCTAGG+TGG	0.625455	2.2:+25272390	MS.gene045736:CDS
TGAGAACTTTGTTTGGACTG+TGG	0.632658	2.2:+25273054	MS.gene045736:CDS
TTGGAACATCCACTGCATTG+GGG	0.645337	2.2:+25272365	MS.gene045736:CDS
CACTTGAGTTCAGACTCTTG+AGG	0.649831	2.2:+25271929	MS.gene045736:CDS
TCAATATTTCATAGATACTG+CGG	0.658525	2.2:+25274132	MS.gene045736:CDS
AACCACCTAGCCTCCCATCG+AGG	0.678896	2.2:-25272392	None:intergenic
GCATTGTTTATGTATCATCG+TGG	0.679800	2.2:+25274172	MS.gene045736:CDS
GTACGATGTGTATGGCACAT+TGG	0.688383	2.2:+25273159	MS.gene045736:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAAAATAATAATTTTTATTA+TGG	-	chr2.2:25272649-25272668	None:intergenic	0.0%
!!	ACAAAAAATTACTTAAAAAT+AGG	-	chr2.2:25271243-25271262	None:intergenic	10.0%
!!	CAAAAAATTACTTAAAAATA+GGG	-	chr2.2:25271242-25271261	None:intergenic	10.0%
!!	TTACATTAAAAAAATTCATA+TGG	+	chr2.2:25270870-25270889	MS.gene045736:intron	10.0%
!!!	AAAAATTCTAATTGTTTTTT+TGG	+	chr2.2:25271409-25271428	MS.gene045736:intron	10.0%
!!!	AAAATTCTAATTGTTTTTTT+GGG	+	chr2.2:25271410-25271429	MS.gene045736:intron	10.0%
!!!	TAAAAAAATCTTTTCAAAAA+TGG	+	chr2.2:25270956-25270975	MS.gene045736:intron	10.0%
!!!	TACAAATTAACAATAATTTT+CGG	-	chr2.2:25272100-25272119	None:intergenic	10.0%
!!	AAAAAATTGGTATATCTATA+GGG	-	chr2.2:25271151-25271170	None:intergenic	15.0%
!!	AAAATTTGACATATTTATAG+GGG	+	chr2.2:25271586-25271605	MS.gene045736:intron	15.0%
!!	AACAATTAGTGAAAAATTTA+AGG	-	chr2.2:25273941-25273960	None:intergenic	15.0%
!!	ATATGCATTTACTTAATAAT+AGG	-	chr2.2:25270724-25270743	None:intergenic	15.0%
!!	ATTGTAATCATTATTATTAG+TGG	+	chr2.2:25273484-25273503	MS.gene045736:intron	15.0%
!!	GAAAATTTGACATATTTATA+GGG	+	chr2.2:25271585-25271604	MS.gene045736:intron	15.0%
!!	TAAATATGAGTTTACTTTAA+AGG	+	chr2.2:25271491-25271510	MS.gene045736:intron	15.0%
!!	TTGTAATCATTATTATTAGT+GGG	+	chr2.2:25273485-25273504	MS.gene045736:intron	15.0%
!!!	AATTTTGAGATAATTTTCTT+AGG	-	chr2.2:25270796-25270815	None:intergenic	15.0%
!!!	ACCATTTTAATAATGAAATT+AGG	+	chr2.2:25271842-25271861	MS.gene045736:CDS	15.0%
!!!	ATGAAAAACAATATTTTGTA+AGG	+	chr2.2:25271069-25271088	MS.gene045736:intron	15.0%
!!!	ATGAATTTTTATATTTTTGG+CGG	-	chr2.2:25271369-25271388	None:intergenic	15.0%
!!!	CATTTATCAATTTTTTTTCT+GGG	+	chr2.2:25271108-25271127	MS.gene045736:intron	15.0%
!!!	CCATTTTAATAATGAAATTA+GGG	+	chr2.2:25271843-25271862	MS.gene045736:CDS	15.0%
!!!	TAAAAAGTATGATTTTTATG+TGG	+	chr2.2:25271046-25271065	MS.gene045736:intron	15.0%
!!!	TATAAAAATCTACTTTTTAC+TGG	-	chr2.2:25272695-25272714	None:intergenic	15.0%
!!!	TTAAGAAATTATTTTTGTGT+GGG	+	chr2.2:25270906-25270925	MS.gene045736:intron	15.0%
!!!	TTTCGTAAATATTTAAAATC+TGG	-	chr2.2:25273094-25273113	None:intergenic	15.0%
!!	AGATAACAGTAACAAAAATA+TGG	-	chr2.2:25270842-25270861	None:intergenic	20.0%
!!	ATCATTAAGTAGTGAATATA+AGG	+	chr2.2:25270595-25270614	MS.gene045736:intron	20.0%
!!	CAAAAAATTGGTATATCTAT+AGG	-	chr2.2:25271152-25271171	None:intergenic	20.0%
!!	CAATAAAATTAAGCATTAGT+CGG	-	chr2.2:25272964-25272983	None:intergenic	20.0%
!!	CCAGAAAAAAAATTGATAAA+TGG	-	chr2.2:25271110-25271129	None:intergenic	20.0%
!!	CCCTAATTTCATTATTAAAA+TGG	-	chr2.2:25271846-25271865	None:intergenic	20.0%
!!	CGAAAATTTGACATATTTAT+AGG	+	chr2.2:25271584-25271603	MS.gene045736:intron	20.0%
!!	GAAATTTGTAATTTCAAGAA+AGG	+	chr2.2:25272176-25272195	MS.gene045736:CDS	20.0%
!!	GTATGTGAGTAAAAAATAAA+GGG	-	chr2.2:25271628-25271647	None:intergenic	20.0%
!!	TAATGATTGTATATGATCAT+AGG	-	chr2.2:25270582-25270601	None:intergenic	20.0%
!!	TGTATGTGAGTAAAAAATAA+AGG	-	chr2.2:25271629-25271648	None:intergenic	20.0%
!!!	AAAATCTTTTCAAAAATGGA+AGG	+	chr2.2:25270960-25270979	MS.gene045736:intron	20.0%
!!!	AAATTTTCAATCACTACTTT+TGG	+	chr2.2:25271215-25271234	MS.gene045736:intron	20.0%
!!!	AAGTGTAGATAAAAAACTTT+TGG	+	chr2.2:25271523-25271542	MS.gene045736:intron	20.0%
!!!	AGAAAACTTTTCAAATAAAG+TGG	-	chr2.2:25273254-25273273	None:intergenic	20.0%
!!!	AGTGTAGATAAAAAACTTTT+GGG	+	chr2.2:25271524-25271543	MS.gene045736:intron	20.0%
!!!	CCATTTATCAATTTTTTTTC+TGG	+	chr2.2:25271107-25271126	MS.gene045736:intron	20.0%
!!!	CTTAAGAAATTATTTTTGTG+TGG	+	chr2.2:25270905-25270924	MS.gene045736:intron	20.0%
!!!	CTTTTAGAGATTTTTTTCTA+AGG	+	chr2.2:25272593-25272612	MS.gene045736:intron	20.0%
!!!	GACTAATGCTTAATTTTATT+GGG	+	chr2.2:25272963-25272982	MS.gene045736:intron	20.0%
!!!	GCATAATAATTTTTTAGAAC+TGG	-	chr2.2:25270687-25270706	None:intergenic	20.0%
!!!	TAAAGGGTTAAATATGTTTT+TGG	-	chr2.2:25271612-25271631	None:intergenic	20.0%
!!!	TAAGAAATTATTTTTGTGTG+GGG	+	chr2.2:25270907-25270926	MS.gene045736:intron	20.0%
!!!	TAATATATGCTTCCAAATTT+TGG	-	chr2.2:25272075-25272094	None:intergenic	20.0%
!!!	TAATGTTTTGTACATACAAT+AGG	-	chr2.2:25274265-25274284	None:intergenic	20.0%
!!!	TAGTGATTGAAAATTTTGTA+GGG	-	chr2.2:25271211-25271230	None:intergenic	20.0%
!!!	TTCTAAAATTCGATAACAAA+TGG	+	chr2.2:25272753-25272772	MS.gene045736:intron	20.0%
!!!	TTCTGGGTTAAATATATTTT+TGG	+	chr2.2:25271124-25271143	MS.gene045736:intron	20.0%
!!!	TTTACTTGAATTTGCTAAAT+TGG	+	chr2.2:25272454-25272473	MS.gene045736:CDS	20.0%
!	AAGAATGATGCTTAACAAAA+CGG	+	chr2.2:25271762-25271781	MS.gene045736:CDS	25.0%
!	AGTTTATTGATGTATTGCAA+AGG	+	chr2.2:25271806-25271825	MS.gene045736:CDS	25.0%
!	ATCATCAATGATGGTAATTA+AGG	-	chr2.2:25273137-25273156	None:intergenic	25.0%
!	ATTTGAAGATGAACCTATAT+TGG	+	chr2.2:25272253-25272272	MS.gene045736:CDS	25.0%
!	CAAATGGAAAAACATTTCTA+AGG	+	chr2.2:25272769-25272788	MS.gene045736:intron	25.0%
!	TAATGAAATTAGGGACATAT+TGG	+	chr2.2:25271852-25271871	MS.gene045736:CDS	25.0%
!	TAGGGATTAAAACTGTTTAT+AGG	+	chr2.2:25271007-25271026	MS.gene045736:intron	25.0%
!	TCAATATTTCATAGATACTG+CGG	+	chr2.2:25274132-25274151	MS.gene045736:CDS	25.0%
!	TGAACATGTAATGTTTATCA+AGG	-	chr2.2:25272558-25272577	None:intergenic	25.0%
!	TTATTGGGTATATTTGAAGA+TGG	+	chr2.2:25272978-25272997	MS.gene045736:intron	25.0%
!	TTCTTATATATTCAATGACC+TGG	-	chr2.2:25271976-25271995	None:intergenic	25.0%
!	TTTACGAAAAGTTATGACAA+AGG	+	chr2.2:25273105-25273124	MS.gene045736:CDS	25.0%
!	TTTGTAATTTCAAGAAAGGT+AGG	+	chr2.2:25272180-25272199	MS.gene045736:CDS	25.0%
!	TTTGTACATACAATAGGAAT+CGG	-	chr2.2:25274259-25274278	None:intergenic	25.0%
!!	AAACACGATTTGTTTCAATA+GGG	+	chr2.2:25270989-25271008	MS.gene045736:intron	25.0%
!!	AAACTTTTCAAATAAAGTGG+AGG	-	chr2.2:25273251-25273270	None:intergenic	25.0%
!!	AAAGCAATGGACAATTTTAA+AGG	+	chr2.2:25271732-25271751	MS.gene045736:CDS	25.0%
!!	AAAGTGAAGAAAACTTTTAG+AGG	+	chr2.2:25271553-25271572	MS.gene045736:intron	25.0%
!!	AAGCATATTTTCATGTAGTT+TGG	-	chr2.2:25273348-25273367	None:intergenic	25.0%
!!	AAGTGAAGAAAACTTTTAGA+GGG	+	chr2.2:25271554-25271573	MS.gene045736:intron	25.0%
!!	AAGTTGAAGGAAAATTTTAG+AGG	-	chr2.2:25271183-25271202	None:intergenic	25.0%
!!	AGCATATTTTCATGTAGTTT+GGG	-	chr2.2:25273347-25273366	None:intergenic	25.0%
!!	AGTTGAAGGAAAATTTTAGA+GGG	-	chr2.2:25271182-25271201	None:intergenic	25.0%
!!	ATACGTTCTTATCTTCATTT+TGG	-	chr2.2:25272325-25272344	None:intergenic	25.0%
!!	CGACTAATGCTTAATTTTAT+TGG	+	chr2.2:25272962-25272981	MS.gene045736:intron	25.0%
!!	GATTTTTGATATCTTTACGA+CGG	+	chr2.2:25272031-25272050	MS.gene045736:intron	25.0%
!!	GTAGTGATTGAAAATTTTGT+AGG	-	chr2.2:25271212-25271231	None:intergenic	25.0%
!!	GTGTAGATAAAAAACTTTTG+GGG	+	chr2.2:25271525-25271544	MS.gene045736:intron	25.0%
!!	TGTAGATAAAAAACTTTTGG+GGG	+	chr2.2:25271526-25271545	MS.gene045736:intron	25.0%
!!	TTCTTTGCACTTTTATCTTA+GGG	+	chr2.2:25270646-25270665	MS.gene045736:intron	25.0%
!!	TTTGAAACTTTGAAAAAGTG+TGG	+	chr2.2:25273402-25273421	MS.gene045736:CDS	25.0%
!!!	AAGTTTTTTATCTACACTTC+TGG	-	chr2.2:25271522-25271541	None:intergenic	25.0%
!!!	ATTTTGTTGATGATTCTAGA+TGG	+	chr2.2:25273298-25273317	MS.gene045736:intron	25.0%
!!!	TTTGAGATAATTTTCTTAGG+AGG	-	chr2.2:25270793-25270812	None:intergenic	25.0%
!!!	TTTTGTTGATGATTCTAGAT+GGG	+	chr2.2:25273299-25273318	MS.gene045736:intron	25.0%
	AACTTACTTTATATGTTCCG+AGG	-	chr2.2:25273880-25273899	None:intergenic	30.0%
	AAGACCTTAAGTATACATCA+AGG	+	chr2.2:25272507-25272526	MS.gene045736:CDS	30.0%
	AAGTAATGTACACAAAGCAA+TGG	+	chr2.2:25271719-25271738	MS.gene045736:CDS	30.0%
	ACTTCAAATAAGTCATGCTT+TGG	+	chr2.2:25272346-25272365	MS.gene045736:CDS	30.0%
	AGACAATTTATGCTTTGCAA+GGG	+	chr2.2:25273021-25273040	MS.gene045736:CDS	30.0%
	AGGTTGAATAGACCAAAATT+TGG	+	chr2.2:25272060-25272079	MS.gene045736:intron	30.0%
	ATGTATACTTAAGGTCTTCT+TGG	-	chr2.2:25272505-25272524	None:intergenic	30.0%
	CATATTTGATTGAAGCAAAG+TGG	+	chr2.2:25273663-25273682	MS.gene045736:CDS	30.0%
	CTAATTTCCAACCATCAATT+TGG	+	chr2.2:25270555-25270574	MS.gene045736:intron	30.0%
	GAGGGACTAAAACAAAAAAT+TGG	-	chr2.2:25271164-25271183	None:intergenic	30.0%
	GATTCCACATTCATTCAAAA+TGG	+	chr2.2:25273781-25273800	MS.gene045736:CDS	30.0%
	GCAATTTGATTCTGAATTTC+AGG	-	chr2.2:25274220-25274239	None:intergenic	30.0%
	TAACACCATCTACTTAATTC+AGG	+	chr2.2:25271696-25271715	MS.gene045736:intron	30.0%
	TAAGCATGAGTAAGTATAGT+AGG	-	chr2.2:25273755-25273774	None:intergenic	30.0%
	TAATTTAGTTGCTCACTTGT+AGG	+	chr2.2:25272909-25272928	MS.gene045736:intron	30.0%
	TACTTCCTGAATTAAGTAGA+TGG	-	chr2.2:25271704-25271723	None:intergenic	30.0%
	TGCTGAAAGTCTAATTATGT+TGG	-	chr2.2:25273834-25273853	None:intergenic	30.0%
	TTCATTCAAAATGGAAGACT+TGG	+	chr2.2:25273790-25273809	MS.gene045736:CDS	30.0%
!	ATGTAGCATTGAATTGACTT+TGG	-	chr2.2:25274007-25274026	None:intergenic	30.0%
!	CAAACACGATTTGTTTCAAT+AGG	+	chr2.2:25270988-25271007	MS.gene045736:intron	30.0%
!	CTTCTTTGCACTTTTATCTT+AGG	+	chr2.2:25270645-25270664	MS.gene045736:intron	30.0%
!	GTTCTTTTTGCTTCTCTAAA+CGG	+	chr2.2:25273897-25273916	MS.gene045736:intron	30.0%
!	TCAATGCAAGGTATCATATA+AGG	+	chr2.2:25270493-25270512	MS.gene045736:intron	30.0%
!	TCTTCCATTTTGAATGAATG+TGG	-	chr2.2:25273788-25273807	None:intergenic	30.0%
!	TTTAAAGCGTGAAAAAGAGA+CGG	+	chr2.2:25273973-25273992	MS.gene045736:intron	30.0%
!!!	CTCTTTTTCACGCTTTAAAA+AGG	-	chr2.2:25273972-25273991	None:intergenic	30.0%
!!!	TAATGCTTACCGCTTTTTTT+AGG	-	chr2.2:25273446-25273465	None:intergenic	30.0%
!!!	TTTTTAGGTATGGAGTGATA+TGG	-	chr2.2:25273431-25273450	None:intergenic	30.0%
	AACATTACATGTTCACGTTG+TGG	+	chr2.2:25272563-25272582	MS.gene045736:intron	35.0%
	AAGGAACTGATACTTGAAGT+TGG	-	chr2.2:25272539-25272558	None:intergenic	35.0%
	AGCACCTTGATGTATACTTA+AGG	-	chr2.2:25272514-25272533	None:intergenic	35.0%
	AGTCCATGATGTTTATAGAG+TGG	+	chr2.2:25274071-25274090	MS.gene045736:CDS	35.0%
	ATCGTACACATCATCAATGA+TGG	-	chr2.2:25273146-25273165	None:intergenic	35.0%
	ATTGATGTATTGCAAAGGCT+TGG	+	chr2.2:25271811-25271830	MS.gene045736:CDS	35.0%
	CAATGCTACATGAATGAAAC+TGG	+	chr2.2:25274016-25274035	MS.gene045736:CDS	35.0%
	CACTCCATACCTAAAAAAAG+CGG	+	chr2.2:25273434-25273453	MS.gene045736:CDS	35.0%
	CATGGTTATGATATATCACC+AGG	+	chr2.2:25271955-25271974	MS.gene045736:CDS	35.0%
	CATTCTCTATGTATTCTCCA+AGG	-	chr2.2:25273712-25273731	None:intergenic	35.0%
	CTAGAAAGAGTTCCAATCAT+AGG	-	chr2.2:25270392-25270411	None:intergenic	35.0%
	CTTCCACTCTATAAACATCA+TGG	-	chr2.2:25274077-25274096	None:intergenic	35.0%
	GAGAATACATAGAGAATGCA+TGG	+	chr2.2:25273714-25273733	MS.gene045736:CDS	35.0%
	GAGACAATTTATGCTTTGCA+AGG	+	chr2.2:25273020-25273039	MS.gene045736:CDS	35.0%
	GATTCTGAATTTCAGGATCT+TGG	-	chr2.2:25274213-25274232	None:intergenic	35.0%
	GCATTGTTTATGTATCATCG+TGG	+	chr2.2:25274172-25274191	MS.gene045736:CDS	35.0%
	TCTCTTGCTTCATCCAATAT+AGG	-	chr2.2:25272269-25272288	None:intergenic	35.0%
	TCTTCACAAAAGCAGTTGAT+AGG	+	chr2.2:25273194-25273213	MS.gene045736:CDS	35.0%
	TGATCATAGGTCCAAATTGA+TGG	-	chr2.2:25270569-25270588	None:intergenic	35.0%
	TGGTGGAAAAGAACTAAACT+TGG	+	chr2.2:25272998-25273017	MS.gene045736:CDS	35.0%
	TTAAAGCGTGAAAAAGAGAC+GGG	+	chr2.2:25273974-25273993	MS.gene045736:intron	35.0%
	TTGCGACTTAATGATAGTTG+TGG	-	chr2.2:25272005-25272024	None:intergenic	35.0%
	TTGGGTATATTTGAAGATGG+TGG	+	chr2.2:25272981-25273000	MS.gene045736:intron	35.0%
	TTGTAGGGACTAAAAGTTGA+AGG	-	chr2.2:25271196-25271215	None:intergenic	35.0%
	TTTATGTATCATCGTGGAGA+TGG	+	chr2.2:25274178-25274197	MS.gene045736:CDS	35.0%
!	GATTGGTTGAGAACTTTGTT+TGG	+	chr2.2:25273047-25273066	MS.gene045736:CDS	35.0%
!!!	AACAGGAGGAAGCATTTTTT+TGG	-	chr2.2:25270420-25270439	None:intergenic	35.0%
!!!	CTTACCGCTTTTTTTAGGTA+TGG	-	chr2.2:25273441-25273460	None:intergenic	35.0%
	AAGTATAGTAGGTGCACCTA+TGG	-	chr2.2:25273744-25273763	None:intergenic	40.0%
	AGGGTTCTACTGTAGCAATA+TGG	+	chr2.2:25270447-25270466	MS.gene045736:CDS	40.0%
	ATGCTTTGCAAGGGATAGAT+TGG	+	chr2.2:25273030-25273049	MS.gene045736:CDS	40.0%
	CTGAATTTCAGGATCTTGGT+TGG	-	chr2.2:25274209-25274228	None:intergenic	40.0%
	CTTCCTCCTGTTGAAAAGAA+GGG	+	chr2.2:25270428-25270447	MS.gene045736:CDS	40.0%
	GAGAATGCATGGATATCCAT+AGG	+	chr2.2:25273725-25273744	MS.gene045736:CDS	40.0%
	GATGATGTGTACGATGTGTA+TGG	+	chr2.2:25273151-25273170	MS.gene045736:CDS	40.0%
	TAGATGGGATCTAAATGCCA+TGG	+	chr2.2:25273314-25273333	MS.gene045736:intron	40.0%
	TATCTTTACGACGGTGATTG+AGG	+	chr2.2:25272040-25272059	MS.gene045736:intron	40.0%
	TCTATGTATTCTCCAAGGCT+TGG	-	chr2.2:25273707-25273726	None:intergenic	40.0%
	TGCGGTAAATCTTGCTAGAA+TGG	+	chr2.2:25274150-25274169	MS.gene045736:CDS	40.0%
!	CATAGGTCCAAATTGATGGT+TGG	-	chr2.2:25270565-25270584	None:intergenic	40.0%
!	GAGAACTTTGTTTGGACTGT+GGG	+	chr2.2:25273055-25273074	MS.gene045736:CDS	40.0%
!	GTAGAACCCTTCTTTTCAAC+AGG	-	chr2.2:25270437-25270456	None:intergenic	40.0%
!	TGAGAACTTTGTTTGGACTG+TGG	+	chr2.2:25273054-25273073	MS.gene045736:CDS	40.0%
!	TTGAGTTGTGCGATGGAAAT+TGG	-	chr2.2:25270477-25270496	None:intergenic	40.0%
!	TTTCAGGATCTTGGTTGGTA+TGG	-	chr2.2:25274204-25274223	None:intergenic	40.0%
!	TTTGGAACATCCACTGCATT+GGG	+	chr2.2:25272364-25272383	MS.gene045736:CDS	40.0%
!!	GATTGGAACTCTTTCTAGCT+TGG	+	chr2.2:25270394-25270413	MS.gene045736:CDS	40.0%
	ATGTTCCGAGGTCATTAGCA+AGG	-	chr2.2:25273868-25273887	None:intergenic	45.0%
	CACTTGAGTTCAGACTCTTG+AGG	+	chr2.2:25271929-25271948	MS.gene045736:CDS	45.0%
	GAAGCAAAGTGGTACCATAG+TGG	+	chr2.2:25273674-25273693	MS.gene045736:CDS	45.0%
	GCTTCCTCCTGTTGAAAAGA+AGG	+	chr2.2:25270427-25270446	MS.gene045736:CDS	45.0%
	GTACGATGTGTATGGCACAT+TGG	+	chr2.2:25273159-25273178	MS.gene045736:CDS	45.0%
	GTAGTTTGGGAGAGAATCCA+TGG	-	chr2.2:25273334-25273353	None:intergenic	45.0%
	TTACGCCTTGCTAATGACCT+CGG	+	chr2.2:25273860-25273879	MS.gene045736:CDS	45.0%
	TTCAGACTCTTGAGGCAACA+TGG	+	chr2.2:25271937-25271956	MS.gene045736:CDS	45.0%
	TTGGAACATCCACTGCATTG+GGG	+	chr2.2:25272365-25272384	MS.gene045736:CDS	45.0%
!	GAACCCTTCTTTTCAACAGG+AGG	-	chr2.2:25270434-25270453	None:intergenic	45.0%
!!	AACTGGAGCTTCTGAAGTTG+AGG	+	chr2.2:25274033-25274052	MS.gene045736:CDS	45.0%
!!	AGGTAGGTTTGTTGACGTTG+AGG	+	chr2.2:25272196-25272215	MS.gene045736:CDS	45.0%
!!	CTTTGGAACATCCACTGCAT+TGG	+	chr2.2:25272363-25272382	MS.gene045736:CDS	45.0%
!!	GGTAGGTTTGTTGACGTTGA+GGG	+	chr2.2:25272197-25272216	MS.gene045736:CDS	45.0%
!!!	AATATGAATTTTTATATTTT+TGG	-	chr2.2:25271372-25271391	None:intergenic	5.0%
!!!	ATTATTATTATTTTAATAGT+GGG	+	chr2.2:25273625-25273644	MS.gene045736:intron	5.0%
!!!	TATTATTATTATTTTAATAG+TGG	+	chr2.2:25273624-25273643	MS.gene045736:intron	5.0%
!!!	TTAAAAAATTCTAATTTTTT+TGG	-	chr2.2:25271317-25271336	None:intergenic	5.0%
	AGTGGATACACACCAAGCCT+TGG	+	chr2.2:25273692-25273711	MS.gene045736:CDS	50.0%
	CCATCGCACAACTCAATGCA+AGG	+	chr2.2:25270481-25270500	MS.gene045736:CDS	50.0%
!	CCTTGCATTGAGTTGTGCGA+TGG	-	chr2.2:25270484-25270503	None:intergenic	50.0%
!!	GGCTTGGTGTGTATCCACTA+TGG	-	chr2.2:25273691-25273710	None:intergenic	50.0%
	AACCACCTAGCCTCCCATCG+AGG	-	chr2.2:25272395-25272414	None:intergenic	60.0%
	ACCACCTAGCCTCCCATCGA+GGG	-	chr2.2:25272394-25272413	None:intergenic	60.0%
	GCATTGGGGAGTCCCTCGAT+GGG	+	chr2.2:25272379-25272398	MS.gene045736:CDS	60.0%
	TGCATTGGGGAGTCCCTCGA+TGG	+	chr2.2:25272378-25272397	MS.gene045736:CDS	60.0%
	CGAGGGACTCCCCAATGCAG+TGG	-	chr2.2:25272377-25272396	None:intergenic	65.0%
	GAGTCCCTCGATGGGAGGCT+AGG	+	chr2.2:25272387-25272406	MS.gene045736:CDS	65.0%
	TCCCTCGATGGGAGGCTAGG+TGG	+	chr2.2:25272390-25272409	MS.gene045736:CDS	65.0%
	TTGGGGAGTCCCTCGATGGG+AGG	+	chr2.2:25272382-25272401	MS.gene045736:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	25270380	25274285	25270380	ID=MS.gene045736
chr2.2	mRNA	25270380	25274285	25270380	ID=MS.gene045736.t1;Parent=MS.gene045736
chr2.2	exon	25270380	25270502	25270380	ID=MS.gene045736.t1.exon1;Parent=MS.gene045736.t1
chr2.2	CDS	25270380	25270502	25270380	ID=cds.MS.gene045736.t1;Parent=MS.gene045736.t1
chr2.2	exon	25271718	25271976	25271718	ID=MS.gene045736.t1.exon2;Parent=MS.gene045736.t1
chr2.2	CDS	25271718	25271976	25271718	ID=cds.MS.gene045736.t1;Parent=MS.gene045736.t1
chr2.2	exon	25272153	25272528	25272153	ID=MS.gene045736.t1.exon3;Parent=MS.gene045736.t1
chr2.2	CDS	25272153	25272528	25272153	ID=cds.MS.gene045736.t1;Parent=MS.gene045736.t1
chr2.2	exon	25272997	25273215	25272997	ID=MS.gene045736.t1.exon4;Parent=MS.gene045736.t1
chr2.2	CDS	25272997	25273215	25272997	ID=cds.MS.gene045736.t1;Parent=MS.gene045736.t1
chr2.2	exon	25273317	25273455	25273317	ID=MS.gene045736.t1.exon5;Parent=MS.gene045736.t1
chr2.2	CDS	25273317	25273455	25273317	ID=cds.MS.gene045736.t1;Parent=MS.gene045736.t1
chr2.2	exon	25273644	25273892	25273644	ID=MS.gene045736.t1.exon6;Parent=MS.gene045736.t1
chr2.2	CDS	25273644	25273892	25273644	ID=cds.MS.gene045736.t1;Parent=MS.gene045736.t1
chr2.2	exon	25273980	25274285	25273980	ID=MS.gene045736.t1.exon7;Parent=MS.gene045736.t1
chr2.2	CDS	25273980	25274285	25273980	ID=cds.MS.gene045736.t1;Parent=MS.gene045736.t1

Gene Sequence

>MS.gene045736

ATGGCTTTACCTATGATTGGAACTCTTTCTAGCTTGGCCAAAAAAATGCTTCCTCCTGTTGAAAAGAAGGGTTCTACTGTAGCAATATGGAACTCCAATTTCCATCGCACAACTCAATGCAAGGTATCATATAAGGATGCAAAAGATTATAGTCAAACAATCACTCGTCGAGTTGCTAATTTCCAACCATCAATTTGGACCTATGATCATATACAATCATTAAGTAGTGAATATAAGGTACTACGATTTAATACTTATTTTGTTTCTTCTTTGCACTTTTATCTTAGGGTTAAGTATATTTTTTCCAGTTCTAAAAAATTATTATGCAAGCAATTTTACTTCCTATTATTAAGTAAATGCATATTTTTGAATGATTTTTGTTGTAATGTTAAGAACATTATAACAAAATTCCTCCTAAGAAAATTATCTCAAAATTTGATTTTTAAATGATCTCTAAGTCCATATTTTTGTTACTGTTATCTTCAATTTTTTACATTAAAAAAATTCATATGGTATTCATCTCAACTTAAGAAATTATTTTTGTGTGGGGAAACTTTTAATAATGTTGAGTACATGTAAAAAAATCTTTTCAAAAATGGAAGGATAGTCAAACACGATTTGTTTCAATAGGGATTAAAACTGTTTATAGGATAATTTCGTAAGAACTAAAAAGTATGATTTTTATGTGGATGAAAAACAATATTTTGTAAGGATAACAACATATTTACCATTTATCAATTTTTTTTCTGGGTTAAATATATTTTTGGTCCCTATAGATATACCAATTTTTTGTTTTAGTCCCTCTAAAATTTTCCTTCAACTTTTAGTCCCTACAAAATTTTCAATCACTACTTTTGGTCCCTATTTTTAAGTAATTTTTTGTATTTTTTAATGAAATTGTGCAGAAATGTGTAGAATATTGTAAAAATCTCTACCAAAAAAATTAGAATTTTTTAACAAAACATAAATTAAATATGAATTTTTAACCGCCAAAAATATAAAAATTCATATTTAATTCATGTTTTGTTAAAAAATTCTAATTGTTTTTTTGGGAGAAATTCTTATAATATTATAAATTTTTCTGCAAAATTTTATTAAGAAACATGAATTCTAAATATGAGTTTACTTTAAAGGAAGAACCAGAAGTGTAGATAAAAAACTTTTGGGGGACTAAAAGTGAAGAAAACTTTTAGAGGGACTAAAACGAAAATTTGACATATTTATAGGGGCCAAAAACATATTTAACCCTTTATTTTTTACTCACATACATATTTATCAAGTCTTCATATATTACAAATCTGAAATGTGAAAAATGCTAACACCATCTACTTAATTCAGGAAGTAATGTACACAAAGCAATGGACAATTTTAAAGGAAAAAGTAAGAATGATGCTTAACAAAACGGAAAATGAGCTTGATCAACTTGAGTTTATTGATGTATTGCAAAGGCTTGGAGTGTCATACCATTTTAATAATGAAATTAGGGACATATTGGATAACATTTACAACAAACAATCATTCAAGTTGAAGAAGAATTTACATGCGACAGCACTTGAGTTCAGACTCTTGAGGCAACATGGTTATGATATATCACCAGGTCATTGAATATATAAGAAAAAGCTCCACAACTATCATTAAGTCGCAAAATTAAGATTTTTGATATCTTTACGACGGTGATTGAGGTTGAATAGACCAAAATTTGGAAGCATATATTACTCCGAAAATTATTGTTAATTTGTATATTAATTTATATTATATAATTATTTGTTGCAGATGTTTTTGTTTGCTTTGAAGATGAAATTTGTAATTTCAAGAAAGGTAGGTTTGTTGACGTTGAGGGAATGTTATCATTGTATGAAGCATCGTTTCATTCATTTGAAGATGAACCTATATTGGATGAAGCAAGAGATGTCACATCAAAGTTTCTCAAAGAATTTTTCGACCAAAATGAAGATAAGAACGTATCACTTCAAATAAGTCATGCTTTGGAACATCCACTGCATTGGGGAGTCCCTCGATGGGAGGCTAGGTGGTTCATTGATATATACGAAAGACAACAAAATAAGAATCATGTTTTACTTGAATTTGCTAAATTGGATTTCAACATTCTTCAAAGCTTCTACCAAGAAGACCTTAAGTATACATCAAGGTGCTAACCAACTTCAAGTATCAGTTCCTTGATAAACATTACATGTTCACGTTGTGGTGTCTAACTTTTAGAGATTTTTTTCTAAGGAAAAAAAAACAGAAATTTTGTGTTTTTTTACCATAATAAAAATTATTATTTTAGAAAAAGATTTATTGAAACTGAACCAGTAAAAAGTAGATTTTTATATAAAGAAATTTTATATTTTCTTCTTCATATTTTTATTTTTCTAAAATTCGATAACAAATGGAAAAACATTTCTAAGGTTGATTAATTATTTTAAAAAATTTATTTTATTAATTTAAATAGTGATTATTTAATTAAAATTATATTTTAAACTAGCTTATTACTTAAAATTTTAACTTAATGAATAGTAAATAAAATAATTTAGTTGCTCACTTGTAGGTATACTAGAGATTGAGATAGATGATACTTCCGACTAATGCTTAATTTTATTGGGTATATTTGAAGATGGTGGAAAAGAACTAAACTTGGAGACAATTTATGCTTTGCAAGGGATAGATTGGTTGAGAACTTTGTTTGGACTGTGGGAACAAATTTCAAACCAGATTTTAAATATTTACGAAAAGTTATGACAAAGGTCAATTCCTTAATTACCATCATTGATGATGTGTACGATGTGTATGGCACATTGGAAGAACTAGAGCTCTTCACAAAAGCAGTTGATAGGTAATTCTTGTTCAATAATTTGTTTTTTACTTCCTCCACTTTATTTGAAAAGTTTTCTTGTTATTAGTGAATAATTATCAATATTTTGTTGATGATTCTAGATGGGATCTAAATGCCATGGATTCTCTCCCAAACTACATGAAAATATGCTTTCATGCACTCTATAACTTTGTGAGTGAACTTGCTTTTGAAACTTTGAAAAAGTGTGGCTACCATATCACTCCATACCTAAAAAAAGCGGTAAGCATTATCATTATCATCATCATTCATTGTAATCATTATTATTAGTGGGCTATTTAGCTTCTTCAAAAAAAAAATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTTAATAGTGGGCAGATCTATGTAAATCATATTTGATTGAAGCAAAGTGGTACCATAGTGGATACACACCAAGCCTTGGAGAATACATAGAGAATGCATGGATATCCATAGGTGCACCTACTATACTTACTCATGCTTACTTTGTGATTCCACATTCATTCAAAATGGAAGACTTGGTTAGTTTAGAAGAAAACTCCAACATAATTAGACTTTCAGCAATAATTTTACGCCTTGCTAATGACCTCGGAACATATAAAGTAAGTTCTTTTTGCTTCTCTAAACGGACTAAAAATGCAATTAAACCTTAAATTTTTCACTAATTGTTTTGCTTTTCCTTTTTAAAGCGTGAAAAAGAGACGGGTGATATTCCAAAGTCAATTCAATGCTACATGAATGAAACTGGAGCTTCTGAAGTTGAGGCTTGTGAGTATGTTAAGTCCATGATGTTTATAGAGTGGAAGAAGTTGAATAAAGAAGCTCATAGTTCATCTTTCTCTCAATATTTCATAGATACTGCGGTAAATCTTGCTAGAATGGCATTGTTTATGTATCATCGTGGAGATGGCCATACCAACCAAGATCCTGAAATTCAGAATCAAATTGCATCATTAGTTTTTCAGCCGATTCCTATTGTATGTACAAAACATTAA

Protein sequence

>MS.gene045736.t1

MALPMIGTLSSLAKKMLPPVEKKGSTVAIWNSNFHRTTQCKEVMYTKQWTILKEKVRMMLNKTENELDQLEFIDVLQRLGVSYHFNNEIRDILDNIYNKQSFKLKKNLHATALEFRLLRQHGYDISPDVFVCFEDEICNFKKGRFVDVEGMLSLYEASFHSFEDEPILDEARDVTSKFLKEFFDQNEDKNVSLQISHALEHPLHWGVPRWEARWFIDIYERQQNKNHVLLEFAKLDFNILQSFYQEDLKYTSRWWKRTKLGDNLCFARDRLVENFVWTVGTNFKPDFKYLRKVMTKVNSLITIIDDVYDVYGTLEELELFTKAVDRWDLNAMDSLPNYMKICFHALYNFVSELAFETLKKCGYHITPYLKKAWADLCKSYLIEAKWYHSGYTPSLGEYIENAWISIGAPTILTHAYFVIPHSFKMEDLVSLEENSNIIRLSAIILRLANDLGTYKREKETGDIPKSIQCYMNETGASEVEACEYVKSMMFIEWKKLNKEAHSSSFSQYFIDTAVNLARMALFMYHRGDGHTNQDPEIQNQIASLVFQPIPIVCTKH