AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77594

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77594.t1 MTR_2g064425 68.689 412 87 1 3 372 174 585 0.0 592
MS.gene77594.t1 MTR_2g064425 68.689 412 87 1 3 372 159 570 0.0 592
MS.gene77594.t1 MTR_2g065450 63.438 413 108 2 3 372 177 589 0.0 528
MS.gene77594.t1 MTR_8g057020 60.533 413 120 2 3 372 176 588 0.0 519
MS.gene77594.t1 MTR_4g045810 40.291 412 201 4 3 370 168 578 1.49e-100 309
MS.gene77594.t1 MTR_2g064425 63.675 234 43 1 3 194 159 392 2.63e-100 304
MS.gene77594.t1 MTR_2g064425 63.675 234 43 1 3 194 174 407 4.00e-100 304
MS.gene77594.t1 MTR_7g010710 33.659 410 223 6 9 371 146 553 1.02e-83 265
MS.gene77594.t1 MTR_2g089130 35.593 413 220 5 3 372 154 563 1.99e-80 257
MS.gene77594.t1 MTR_2g089120 34.541 414 224 6 3 372 154 564 2.69e-77 249
MS.gene77594.t1 MTR_4g081460 29.157 415 248 5 3 373 150 562 6.04e-58 197
MS.gene77594.t1 MTR_6g039440 27.952 415 253 5 3 373 141 553 1.80e-52 183
MS.gene77594.t1 MTR_4g092020 27.404 416 243 10 3 368 160 566 8.39e-52 181
MS.gene77594.t1 MTR_4g048460 29.398 415 247 5 3 373 149 561 1.69e-51 181
MS.gene77594.t1 MTR_4g092010 29.231 390 226 7 9 353 158 542 1.21e-49 176
MS.gene77594.t1 MTR_6g008560 27.229 415 256 5 3 373 145 557 1.28e-49 175
MS.gene77594.t1 MTR_5g094620 28.019 414 254 3 3 373 125 537 1.63e-49 175
MS.gene77594.t1 MTR_5g073200 27.536 414 254 5 4 373 138 549 4.99e-48 171
MS.gene77594.t1 MTR_2g010960 28.744 414 245 8 2 367 106 517 2.29e-47 169
MS.gene77594.t1 MTR_2g082010 25.714 420 263 6 3 376 155 571 3.15e-47 169
MS.gene77594.t1 MTR_6g008560 26.551 403 250 5 15 373 1 401 8.02e-47 164
MS.gene77594.t1 MTR_6g064980 27.603 413 250 7 2 367 102 512 1.43e-45 164
MS.gene77594.t1 MTR_3g063120 28.087 413 248 7 2 367 78 488 2.57e-45 162
MS.gene77594.t1 MTR_5g062230 26.329 414 261 3 3 373 141 553 5.21e-45 163
MS.gene77594.t1 MTR_2g082050 26.772 381 263 6 3 370 79 456 1.08e-44 160
MS.gene77594.t1 MTR_6g065040 27.586 406 240 9 4 361 2 401 2.23e-44 158
MS.gene77594.t1 MTR_3g063170 27.536 414 249 9 2 367 102 512 5.37e-44 159
MS.gene77594.t1 MTR_3g465090 26.812 414 252 9 2 367 102 512 8.33e-44 159
MS.gene77594.t1 MTR_3g464190 26.812 414 259 3 3 373 145 557 4.25e-43 157
MS.gene77594.t1 MTR_8g007515 25.416 421 265 6 1 374 139 557 6.10e-43 157
MS.gene77594.t1 MTR_3g063140 27.470 415 248 9 2 367 102 512 8.36e-43 156
MS.gene77594.t1 MTR_2g081980 25.061 411 262 4 9 374 149 558 3.28e-42 155
MS.gene77594.t1 MTR_3g052120 27.407 405 243 9 11 367 111 512 7.07e-41 151
MS.gene77594.t1 MTR_4g048460 31.646 316 171 4 3 275 149 462 4.37e-39 145
MS.gene77594.t1 MTR_5g073260 25.301 415 264 5 3 373 140 552 2.28e-38 144
MS.gene77594.t1 MTR_2g082060 23.934 422 259 8 3 376 149 556 3.80e-35 135
MS.gene77594.t1 MTR_4g019550 23.855 415 266 6 3 373 150 558 1.06e-29 120
MS.gene77594.t1 MTR_5g094530 34.286 175 106 2 28 193 4 178 2.95e-27 107
MS.gene77594.t1 MTR_5g081730 46.875 96 50 1 21 116 16 110 2.36e-19 84.3
MS.gene77594.t1 MTR_2g012870 24.695 328 187 8 12 283 341 664 1.88e-17 84.7
MS.gene77594.t1 MTR_3g058160 23.607 377 213 12 3 311 343 712 4.93e-17 83.2
MS.gene77594.t1 MTR_3g058160 23.607 377 213 12 3 311 139 508 5.11e-17 83.2
MS.gene77594.t1 MTR_3g058160 23.607 377 213 12 3 311 350 719 5.62e-17 83.2
MS.gene77594.t1 MTR_3g058160 23.607 377 213 12 3 311 330 699 5.81e-17 83.2
MS.gene77594.t1 MTR_3g058160 25.660 265 147 9 93 311 480 740 4.38e-16 80.5
MS.gene77594.t1 MTR_2g064295 22.478 347 197 11 3 283 330 670 5.22e-15 77.0
MS.gene77594.t1 MTR_7g057340 23.837 172 130 1 203 373 1 172 1.23e-12 65.9
MS.gene77594.t1 MTR_7g050990 40.789 76 45 0 61 136 29 104 5.80e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77594.t1 AT3G25810 39.141 419 202 7 8 373 179 597 2.57e-103 317
MS.gene77594.t1 AT3G25830 38.889 414 202 6 11 373 181 594 7.34e-102 313
MS.gene77594.t1 AT3G25820 38.889 414 202 6 11 373 181 594 7.34e-102 313
MS.gene77594.t1 AT4G16730 37.116 423 213 6 4 373 71 493 2.12e-97 298
MS.gene77594.t1 AT4G16730 37.116 423 213 6 4 373 117 539 4.77e-97 299
MS.gene77594.t1 AT4G16740 37.317 410 207 6 11 373 158 564 1.05e-95 296
MS.gene77594.t1 AT2G24210 38.051 431 205 12 1 373 164 590 2.38e-94 294
MS.gene77594.t1 AT1G61680 27.612 402 242 7 3 356 155 555 1.79e-50 178
MS.gene77594.t1 AT1G61680 27.612 402 242 7 3 356 162 562 2.26e-50 177
MS.gene77594.t1 AT3G25820 35.223 247 115 3 11 212 181 427 5.46e-49 171
MS.gene77594.t1 AT5G23960 26.506 415 258 7 3 373 134 545 1.38e-42 156
MS.gene77594.t1 AT5G23960 26.379 417 258 7 3 373 134 547 3.15e-42 155
MS.gene77594.t1 AT4G16740 35.217 230 103 3 11 194 158 387 2.15e-40 147
MS.gene77594.t1 AT3G14520 24.700 417 267 4 4 373 188 604 4.73e-39 147
MS.gene77594.t1 AT1G70080 27.184 412 252 4 3 366 193 604 5.29e-39 147
MS.gene77594.t1 AT1G31950 24.460 417 268 3 4 373 190 606 8.72e-39 146
MS.gene77594.t1 AT1G31950 24.460 417 268 3 4 373 191 607 9.24e-39 146
MS.gene77594.t1 AT3G14540 25.768 423 255 7 4 373 185 601 1.92e-37 142
MS.gene77594.t1 AT3G29190 26.555 418 259 7 3 373 185 601 1.05e-35 137
MS.gene77594.t1 AT3G29410 24.344 419 265 5 3 373 84 498 1.53e-35 136
MS.gene77594.t1 AT1G33750 24.941 421 259 6 3 373 190 603 4.02e-35 136
MS.gene77594.t1 AT3G29410 24.344 419 265 5 3 373 189 603 4.23e-35 136
MS.gene77594.t1 AT3G14490 24.762 420 262 7 4 373 186 601 1.03e-33 132
MS.gene77594.t1 AT3G25820 43.066 137 75 2 11 144 181 317 9.44e-33 127
MS.gene77594.t1 AT3G25820 43.066 137 75 2 11 144 181 317 9.44e-33 127
MS.gene77594.t1 AT3G32030 23.373 415 260 7 11 373 196 604 3.26e-32 128
MS.gene77594.t1 AT3G29110 25.672 409 258 5 4 366 182 590 3.33e-31 125
MS.gene77594.t1 AT4G16740 42.254 142 78 2 11 148 158 299 1.24e-29 117
MS.gene77594.t1 AT4G16740 42.254 142 78 2 11 148 158 299 1.24e-29 117
MS.gene77594.t1 AT1G31950 24.138 319 197 2 4 277 191 509 1.11e-27 114
MS.gene77594.t1 AT4G20230 22.573 412 273 4 3 368 200 611 1.71e-27 114
MS.gene77594.t1 AT4G20230 22.573 412 273 4 3 368 188 599 1.76e-27 114
MS.gene77594.t1 AT3G14540 26.154 325 183 6 4 277 185 503 4.79e-27 112
MS.gene77594.t1 AT4G15870 22.927 410 258 7 3 366 194 591 9.35e-27 112
MS.gene77594.t1 AT5G44630 24.578 415 263 7 4 370 142 554 5.70e-26 109
MS.gene77594.t1 AT1G48800 24.762 420 249 10 4 366 190 599 4.31e-25 107
MS.gene77594.t1 AT1G66020 22.927 410 270 5 3 366 182 591 2.07e-24 105
MS.gene77594.t1 AT4G20200 22.439 410 272 4 3 366 188 597 2.12e-24 105
MS.gene77594.t1 AT5G48110 23.582 335 208 5 79 367 5 337 1.29e-23 100
MS.gene77594.t1 AT1G48820 25.549 364 210 6 3 321 163 510 2.50e-23 102
MS.gene77594.t1 AT2G23230 24.561 399 249 7 4 353 185 580 3.06e-23 102
MS.gene77594.t1 AT2G23230 24.495 396 248 7 4 353 185 575 3.58e-23 101
MS.gene77594.t1 AT4G20210 24.524 420 265 9 3 373 184 600 1.11e-22 100
MS.gene77594.t1 AT4G13300 22.222 414 276 6 3 370 138 551 2.39e-22 99.0
MS.gene77594.t1 AT4G13280 22.410 415 274 7 3 370 138 551 5.46e-22 97.8
MS.gene77594.t1 AT4G13280 24.242 396 266 10 3 373 138 524 1.16e-16 82.0
MS.gene77594.t1 AT1G66020 32.414 145 96 2 3 145 182 326 2.86e-16 80.5
MS.gene77594.t1 AT1G79460 22.454 383 233 9 52 374 402 780 5.68e-16 80.1

Find 52 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACTTCAGTTTGCTAAAT+TGG 0.103435 2.4:+28515709 MS.gene77594:CDS
AAAGTTTCTCAAAGAATATT+TGG 0.167285 2.4:+28515553 MS.gene77594:CDS
ATATGCTCTTATCTTCATTT+TGG 0.204420 2.4:-28515577 None:intergenic
GTTTGAAAATGAAATTATAT+TGG 0.247458 2.4:+28515508 MS.gene77594:CDS
GATTCCACGTTCATTCAAAA+TGG 0.264512 2.4:+28517116 MS.gene77594:CDS
ACATATACTTTAAGTCTTCT+TGG 0.268413 2.4:-28515757 None:intergenic
TATTACTTACTGCTTTCTTT+AGG 0.304141 2.4:-28516875 None:intergenic
ATGTAGCATTGAATTATCTT+TGG 0.305826 2.4:-28517616 None:intergenic
TAACTATGGATAAGTATATC+TGG 0.362564 2.4:-28517087 None:intergenic
CTTACTGCTTTCTTTAGGTA+TGG 0.402220 2.4:-28516870 None:intergenic
ACTTCAAATAAGTCATGCTT+TGG 0.406823 2.4:+28515601 MS.gene77594:CDS
AAGAAAACTCAAACATGATT+AGG 0.407501 2.4:+28517157 MS.gene77594:CDS
TACTCAACACAACATTGAAT+AGG 0.408865 2.4:-28517874 None:intergenic
GTGGAATCAGAAAGTAACTA+TGG 0.412857 2.4:-28517101 None:intergenic
ATATTCTTTGAGAAACTTTG+AGG 0.413496 2.4:-28515549 None:intergenic
TGCTTTGAACATTGGTAGAA+TGG 0.422510 2.4:+28517762 MS.gene77594:CDS
AAGTATATCTGGTGCACCTA+TGG 0.431316 2.4:-28517076 None:intergenic
TTACGCCTTGCTAATGACCT+TGG 0.465962 2.4:+28517195 MS.gene77594:CDS
CTTTGGAGCTTCCACTACAT+TGG 0.473873 2.4:+28515618 MS.gene77594:CDS
TTTCTCGATGGGAGGCTCAA+TGG 0.484973 2.4:+28515645 MS.gene77594:CDS
TCTATGTATTCTTCAAGACT+TGG 0.487482 2.4:-28517039 None:intergenic
ATAGATACTGCTTTGAACAT+TGG 0.501453 2.4:+28517754 MS.gene77594:CDS
TTCATTCAAAATGGAAGACT+TGG 0.509358 2.4:+28517125 MS.gene77594:CDS
AACACAACATTGAATAGGAA+AGG 0.527983 2.4:-28517869 None:intergenic
GGCCAATCTGTTGATGTTGA+GGG 0.533101 2.4:+28515452 MS.gene77594:CDS
TTCCCTCAACATCAACAGAT+TGG 0.534301 2.4:-28515454 None:intergenic
AGGCCAATCTGTTGATGTTG+AGG 0.541057 2.4:+28515451 MS.gene77594:CDS
AAGAATACATAGAGAATGCA+TGG 0.541704 2.4:+28517049 MS.gene77594:CDS
TTTCAGCGTGAAAATGAGAT+GGG 0.542662 2.4:+28517586 MS.gene77594:intron
ACACAACATTGAATAGGAAA+GGG 0.549956 2.4:-28517868 None:intergenic
AGACTTGAAATTTGTTCCCA+CGG 0.551562 2.4:-28516481 None:intergenic
CGAGAAATTCTCCAATGTAG+TGG 0.558830 2.4:-28515629 None:intergenic
TACATTGGAGAATTTCTCGA+TGG 0.572320 2.4:+28515633 MS.gene77594:CDS
CTTCCATGTTGTACACAACA+AGG 0.573406 2.4:-28517686 None:intergenic
TAGATGGGATCTAAATGCCA+TGG 0.574694 2.4:+28516746 MS.gene77594:intron
GCATTGTTCATGTACCTACA+TGG 0.575275 2.4:+28517784 MS.gene77594:CDS
GTATTGTGGGAGAGGTTCCA+TGG 0.575761 2.4:-28516763 None:intergenic
GAGAATGCATGGATATCCAT+AGG 0.583758 2.4:+28517060 MS.gene77594:CDS
AACTTACTTTATAAGATCCA+AGG 0.584705 2.4:-28517212 None:intergenic
TCGTGGAGCTTCTGAAGTCG+AGG 0.598317 2.4:+28517645 MS.gene77594:CDS
CAATGCTACATGAACGATCG+TGG 0.605163 2.4:+28517628 MS.gene77594:CDS
AAGATCCAAGGTCATTAGCA+AGG 0.609710 2.4:-28517200 None:intergenic
TGGTGGAAAAGAATCGAACT+TGG 0.611617 2.4:+28516408 MS.gene77594:CDS
GAAGCAAGGTGGTATCATAG+TGG 0.612090 2.4:+28517009 MS.gene77594:CDS
TTCATGTACCTACATGGAGA+TGG 0.612512 2.4:+28517790 MS.gene77594:CDS
TTGGAGAATTTCTCGATGGG+AGG 0.615953 2.4:+28515637 MS.gene77594:CDS
AAGACTTAAAGTATATGTCA+AGG 0.616611 2.4:+28515762 MS.gene77594:CDS
ACATTGGAGAATTTCTCGAT+GGG 0.625313 2.4:+28515634 MS.gene77594:CDS
ATGCATATTTGATAGAAGCA+AGG 0.628575 2.4:+28516995 MS.gene77594:CDS
TGCTATGACCATCTCCATGT+AGG 0.637310 2.4:-28517798 None:intergenic
AGTCCTTGTTGTGTACAACA+TGG 0.651967 2.4:+28517683 MS.gene77594:CDS
CATATTTGATAGAAGCAAGG+TGG 0.695260 2.4:+28516998 MS.gene77594:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTGAATTAACTTAAATT+TGG + chr2.4:28515924-28515943 MS.gene77594:intron 10.0%
!! TCAATTATAAATTTAATCAA+TGG + chr2.4:28516271-28516290 MS.gene77594:intron 10.0%
!! GTTTGAAAATGAAATTATAT+TGG + chr2.4:28515508-28515527 MS.gene77594:CDS 15.0%
!!! ACATTTAAAATTGTGAATTT+TGG + chr2.4:28515900-28515919 MS.gene77594:intron 15.0%
!!! ATTACTTTTAAATGAGAATA+AGG - chr2.4:28516686-28516705 None:intergenic 15.0%
!!! ATTGTATTAATTTTTTTACG+CGG - chr2.4:28517459-28517478 None:intergenic 15.0%
!!! TCTATTTATTATGAGTTTTT+AGG + chr2.4:28516921-28516940 MS.gene77594:intron 15.0%
!!! TTACTTTTAAATGAGAATAA+GGG - chr2.4:28516685-28516704 None:intergenic 15.0%
!! AAAGTTTCTCAAAGAATATT+TGG + chr2.4:28515553-28515572 MS.gene77594:CDS 20.0%
!! AAATCAGGATATATAGTATA+TGG - chr2.4:28516108-28516127 None:intergenic 20.0%
!! AACAATTAGTGATAAGTTAA+AGG - chr2.4:28517553-28517572 None:intergenic 20.0%
!! AACGTTTAAAAAAATAACGA+AGG - chr2.4:28517273-28517292 None:intergenic 20.0%
!! AGATCATTTATTGTAAAATC+AGG - chr2.4:28516123-28516142 None:intergenic 20.0%
!! ATCATAAATATCATCAATTG+TGG - chr2.4:28516555-28516574 None:intergenic 20.0%
!! ATGTATCATAACAATAATGA+AGG - chr2.4:28517297-28517316 None:intergenic 20.0%
!! TTAAATTGTATGTATGAAGA+AGG - chr2.4:28517530-28517549 None:intergenic 20.0%
!!! ATTTTCTCTCAAATTTTTTG+TGG - chr2.4:28517325-28517344 None:intergenic 20.0%
!!! GAATTGTTGAAAATTTTGTT+TGG + chr2.4:28516457-28516476 MS.gene77594:CDS 20.0%
!!! GATTTTATGTGTTTTTATAG+TGG + chr2.4:28516959-28516978 MS.gene77594:intron 20.0%
!!! TTTTTTTGTTCTTTTTAAGC+TGG + chr2.4:28516388-28516407 MS.gene77594:intron 20.0%
! AACTTACTTTATAAGATCCA+AGG - chr2.4:28517215-28517234 None:intergenic 25.0%
! AAGAAAACTCAAACATGATT+AGG + chr2.4:28517157-28517176 MS.gene77594:CDS 25.0%
! AAGACTTAAAGTATATGTCA+AGG + chr2.4:28515762-28515781 MS.gene77594:CDS 25.0%
! ACATATACTTTAAGTCTTCT+TGG - chr2.4:28515760-28515779 None:intergenic 25.0%
! ATGAAGAATATTTGCAAATG+AGG + chr2.4:28517369-28517388 MS.gene77594:intron 25.0%
! ATTAATACAATGAGAATTGC+TGG + chr2.4:28517468-28517487 MS.gene77594:intron 25.0%
! GTTAATCACATGTAAAATTG+TGG + chr2.4:28515844-28515863 MS.gene77594:intron 25.0%
! TAACTATGGATAAGTATATC+TGG - chr2.4:28517090-28517109 None:intergenic 25.0%
! TATTACTTACTGCTTTCTTT+AGG - chr2.4:28516878-28516897 None:intergenic 25.0%
! TTGAAACCTTGAAAAAAAGT+GGG + chr2.4:28516835-28516854 MS.gene77594:CDS 25.0%
! TTTGTAAGGAATAATTATGC+TGG - chr2.4:28517239-28517258 None:intergenic 25.0%
!! AAGCATATTTTCATGTATTG+TGG - chr2.4:28516780-28516799 None:intergenic 25.0%
!! AGCATATTTTCATGTATTGT+GGG - chr2.4:28516779-28516798 None:intergenic 25.0%
!! AGGAAGAGATTAATTTTGTA+AGG - chr2.4:28517253-28517272 None:intergenic 25.0%
!! ATACCTTGGTTATATCTTTT+CGG - chr2.4:28516520-28516539 None:intergenic 25.0%
!! ATATGCTCTTATCTTCATTT+TGG - chr2.4:28515580-28515599 None:intergenic 25.0%
!! ATATTCTTTGAGAAACTTTG+AGG - chr2.4:28515552-28515571 None:intergenic 25.0%
!! ATGTAGCATTGAATTATCTT+TGG - chr2.4:28517619-28517638 None:intergenic 25.0%
!! ATTATGCGATTTTGAATTTC+AGG - chr2.4:28517832-28517851 None:intergenic 25.0%
!! TTATGCGATTTTGAATTTCA+GGG - chr2.4:28517831-28517850 None:intergenic 25.0%
!! TTTGAAACCTTGAAAAAAAG+TGG + chr2.4:28516834-28516853 MS.gene77594:CDS 25.0%
!!! TTTACTTCAGTTTGCTAAAT+TGG + chr2.4:28515709-28515728 MS.gene77594:CDS 25.0%
AAAGTAGTCATTACGTTTCA+TGG + chr2.4:28515789-28515808 MS.gene77594:intron 30.0%
AACACAACATTGAATAGGAA+AGG - chr2.4:28517872-28517891 None:intergenic 30.0%
AAGAATACATAGAGAATGCA+TGG + chr2.4:28517049-28517068 MS.gene77594:CDS 30.0%
ACACAACATTGAATAGGAAA+GGG - chr2.4:28517871-28517890 None:intergenic 30.0%
ACTTCAAATAAGTCATGCTT+TGG + chr2.4:28515601-28515620 MS.gene77594:CDS 30.0%
ATACCGAAAAGATATAACCA+AGG + chr2.4:28516514-28516533 MS.gene77594:intron 30.0%
ATCATCAATTGTGGTAATCA+AGG - chr2.4:28516546-28516565 None:intergenic 30.0%
ATGCATATTTGATAGAAGCA+AGG + chr2.4:28516995-28517014 MS.gene77594:CDS 30.0%
CAATTACCTATCAATTGCTT+TGG - chr2.4:28516612-28516631 None:intergenic 30.0%
GATGATATTTATGATGTGCA+TGG + chr2.4:28516560-28516579 MS.gene77594:intron 30.0%
GGTTAATTCATTTGACAAGA+TGG + chr2.4:28515865-28515884 MS.gene77594:intron 30.0%
GTTCACAATATTTCCATCTT+GGG + chr2.4:28517418-28517437 MS.gene77594:intron 30.0%
TACTCAACACAACATTGAAT+AGG - chr2.4:28517877-28517896 None:intergenic 30.0%
TCTATGTATTCTTCAAGACT+TGG - chr2.4:28517042-28517061 None:intergenic 30.0%
TGTTCACAATATTTCCATCT+TGG + chr2.4:28517417-28517436 MS.gene77594:intron 30.0%
TTCACGCTGAAAAAAGAAAA+AGG - chr2.4:28517578-28517597 None:intergenic 30.0%
TTCATTCAAAATGGAAGACT+TGG + chr2.4:28517125-28517144 MS.gene77594:CDS 30.0%
! ATAGATACTGCTTTGAACAT+TGG + chr2.4:28517754-28517773 MS.gene77594:CDS 30.0%
! ATTTTCAACAATTCTATCCC+TGG - chr2.4:28516451-28516470 None:intergenic 30.0%
! TCTAGAACATTGCAATGATA+TGG - chr2.4:28516233-28516252 None:intergenic 30.0%
! TGAAAAGTTGAGATTTTCCA+GGG + chr2.4:28516431-28516450 MS.gene77594:CDS 30.0%
! TTTTCTTTTGTTCATCGTCT+TGG + chr2.4:28516195-28516214 MS.gene77594:intron 30.0%
!! ATTTGACTTGTGAATCTAGA+TGG + chr2.4:28516730-28516749 MS.gene77594:intron 30.0%
!! TTTGACTTGTGAATCTAGAT+GGG + chr2.4:28516731-28516750 MS.gene77594:intron 30.0%
!! TTTTGTTCTTTTTAAGCTGG+TGG + chr2.4:28516391-28516410 MS.gene77594:intron 30.0%
ACATTGGAGAATTTCTCGAT+GGG + chr2.4:28515634-28515653 MS.gene77594:CDS 35.0%
ACTACGTGAGCACATATTAT+CGG - chr2.4:28516060-28516079 None:intergenic 35.0%
CATATTTGATAGAAGCAAGG+TGG + chr2.4:28516998-28517017 MS.gene77594:CDS 35.0%
CATCGTAGGTGTAGAAAAAT+AGG + chr2.4:28516015-28516034 MS.gene77594:intron 35.0%
CTTACTGCTTTCTTTAGGTA+TGG - chr2.4:28516873-28516892 None:intergenic 35.0%
GATTCCACGTTCATTCAAAA+TGG + chr2.4:28517116-28517135 MS.gene77594:CDS 35.0%
GTGGAATCAGAAAGTAACTA+TGG - chr2.4:28517104-28517123 None:intergenic 35.0%
TACATTGGAGAATTTCTCGA+TGG + chr2.4:28515633-28515652 MS.gene77594:CDS 35.0%
TCTTCACCAAAGCAATTGAT+AGG + chr2.4:28516603-28516622 MS.gene77594:intron 35.0%
TGAGCACATATTATCGGTAA+CGG - chr2.4:28516054-28516073 None:intergenic 35.0%
TTTCAGCGTGAAAATGAGAT+GGG + chr2.4:28517586-28517605 MS.gene77594:intron 35.0%
! AGACTTGAAATTTGTTCCCA+CGG - chr2.4:28516484-28516503 None:intergenic 35.0%
! ATTTTCATGTATTGTGGGAG+AGG - chr2.4:28516774-28516793 None:intergenic 35.0%
! ATTTTTCTACACCTACGATG+CGG - chr2.4:28516015-28516034 None:intergenic 35.0%
! GTGAAAAGTTGAGATTTTCC+AGG + chr2.4:28516430-28516449 MS.gene77594:CDS 35.0%
! TCTTCCATTTTGAATGAACG+TGG - chr2.4:28517123-28517142 None:intergenic 35.0%
! TGCTTTGAACATTGGTAGAA+TGG + chr2.4:28517762-28517781 MS.gene77594:CDS 35.0%
! TTTTCAGCGTGAAAATGAGA+TGG + chr2.4:28517585-28517604 MS.gene77594:intron 35.0%
!! GAAAATTTTGTTTGGACCGT+GGG + chr2.4:28516465-28516484 MS.gene77594:CDS 35.0%
!! TGAAAATTTTGTTTGGACCG+TGG + chr2.4:28516464-28516483 MS.gene77594:CDS 35.0%
!!! ATAGTGCCCACTTTTTTTCA+AGG - chr2.4:28516844-28516863 None:intergenic 35.0%
AAGATCCAAGGTCATTAGCA+AGG - chr2.4:28517203-28517222 None:intergenic 40.0%
AAGTATATCTGGTGCACCTA+TGG - chr2.4:28517079-28517098 None:intergenic 40.0%
CGAGAAATTCTCCAATGTAG+TGG - chr2.4:28515632-28515651 None:intergenic 40.0%
CTTCCATGTTGTACACAACA+AGG - chr2.4:28517689-28517708 None:intergenic 40.0%
CTTGATTTCGTTGTCTAGTG+TGG + chr2.4:28516172-28516191 MS.gene77594:intron 40.0%
GAACACGATCATGACATGAA+AGG - chr2.4:28517402-28517421 None:intergenic 40.0%
GAGAATGCATGGATATCCAT+AGG + chr2.4:28517060-28517079 MS.gene77594:CDS 40.0%
GATAATATGTGCTCACGTAG+TGG + chr2.4:28516059-28516078 MS.gene77594:intron 40.0%
GCATTGTTCATGTACCTACA+TGG + chr2.4:28517784-28517803 MS.gene77594:CDS 40.0%
GGTAATCAAGGCATATACCT+TGG - chr2.4:28516534-28516553 None:intergenic 40.0%
TAGATGGGATCTAAATGCCA+TGG + chr2.4:28516746-28516765 MS.gene77594:intron 40.0%
TGAGAATTGCTGGAGTACAT+CGG + chr2.4:28517478-28517497 MS.gene77594:intron 40.0%
TGGTGGAAAAGAATCGAACT+TGG + chr2.4:28516408-28516427 MS.gene77594:CDS 40.0%
TGTTCATCGTCTTGGTATGT+CGG + chr2.4:28516203-28516222 MS.gene77594:intron 40.0%
TTATGATGTGCATGGTACCT+TGG + chr2.4:28516568-28516587 MS.gene77594:intron 40.0%
TTCATGTACCTACATGGAGA+TGG + chr2.4:28517790-28517809 MS.gene77594:CDS 40.0%
TTCCATCTTGGGACGAATAA+TGG + chr2.4:28517429-28517448 MS.gene77594:intron 40.0%
TTCCATTATTCGTCCCAAGA+TGG - chr2.4:28517434-28517453 None:intergenic 40.0%
TTCCCTCAACATCAACAGAT+TGG - chr2.4:28515457-28515476 None:intergenic 40.0%
! AGTCCTTGTTGTGTACAACA+TGG + chr2.4:28517683-28517702 MS.gene77594:CDS 40.0%
! TTAATTTGAGACCGCATCGT+AGG + chr2.4:28516001-28516020 MS.gene77594:intron 40.0%
AGGCCAATCTGTTGATGTTG+AGG + chr2.4:28515451-28515470 MS.gene77594:CDS 45.0%
CAATGCTACATGAACGATCG+TGG + chr2.4:28517628-28517647 MS.gene77594:CDS 45.0%
GAAGCAAGGTGGTATCATAG+TGG + chr2.4:28517009-28517028 MS.gene77594:CDS 45.0%
GGCCAATCTGTTGATGTTGA+GGG + chr2.4:28515452-28515471 MS.gene77594:CDS 45.0%
TGCTATGACCATCTCCATGT+AGG - chr2.4:28517801-28517820 None:intergenic 45.0%
TGTGATTAACCGACTGTCGT+AGG - chr2.4:28515834-28515853 None:intergenic 45.0%
TTACGCCTTGCTAATGACCT+TGG + chr2.4:28517195-28517214 MS.gene77594:CDS 45.0%
TTGGAGAATTTCTCGATGGG+AGG + chr2.4:28515637-28515656 MS.gene77594:CDS 45.0%
!! CTTTGGAGCTTCCACTACAT+TGG + chr2.4:28515618-28515637 MS.gene77594:CDS 45.0%
!! GAAGAGCTCTAGTTCTTCCA+AGG - chr2.4:28516588-28516607 None:intergenic 45.0%
AACCGACTGTCGTAGGTGTA+GGG - chr2.4:28515827-28515846 None:intergenic 50.0%
GTATTGTGGGAGAGGTTCCA+TGG - chr2.4:28516766-28516785 None:intergenic 50.0%
GTCGTAGGTGTAGGGGAAAT+TGG - chr2.4:28515819-28515838 None:intergenic 50.0%
TAACCGACTGTCGTAGGTGT+AGG - chr2.4:28515828-28515847 None:intergenic 50.0%
TTTCTCGATGGGAGGCTCAA+TGG + chr2.4:28515645-28515664 MS.gene77594:CDS 50.0%
ACCGACTGTCGTAGGTGTAG+GGG - chr2.4:28515826-28515845 None:intergenic 55.0%
TCCCCTACACCTACGACAGT+CGG + chr2.4:28515822-28515841 MS.gene77594:intron 55.0%
!! TCGTGGAGCTTCTGAAGTCG+AGG + chr2.4:28517645-28517664 MS.gene77594:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 28515434 28517897 28515434 ID=MS.gene77594
chr2.4 mRNA 28515434 28517897 28515434 ID=MS.gene77594.t1;Parent=MS.gene77594
chr2.4 exon 28515434 28515783 28515434 ID=MS.gene77594.t1.exon1;Parent=MS.gene77594.t1
chr2.4 CDS 28515434 28515783 28515434 ID=cds.MS.gene77594.t1;Parent=MS.gene77594.t1
chr2.4 exon 28516407 28516499 28516407 ID=MS.gene77594.t1.exon2;Parent=MS.gene77594.t1
chr2.4 CDS 28516407 28516499 28516407 ID=cds.MS.gene77594.t1;Parent=MS.gene77594.t1
chr2.4 exon 28516749 28516887 28516749 ID=MS.gene77594.t1.exon3;Parent=MS.gene77594.t1
chr2.4 CDS 28516749 28516887 28516749 ID=cds.MS.gene77594.t1;Parent=MS.gene77594.t1
chr2.4 exon 28516979 28517227 28516979 ID=MS.gene77594.t1.exon4;Parent=MS.gene77594.t1
chr2.4 CDS 28516979 28517227 28516979 ID=cds.MS.gene77594.t1;Parent=MS.gene77594.t1
chr2.4 exon 28517592 28517897 28517592 ID=MS.gene77594.t1.exon5;Parent=MS.gene77594.t1
chr2.4 CDS 28517592 28517897 28517592 ID=cds.MS.gene77594.t1;Parent=MS.gene77594.t1
Gene Sequence

>MS.gene77594

ATGCGTAATTTTAAGAAAGGCCAATCTGTTGATGTTGAGGGAATGTTATCGATGTATGAAGCATCATTTCATTCGTTTGAAAATGAAATTATATTGGATGAAGCAAGAGATTTGACCTCAAAGTTTCTCAAAGAATATTTGGACCAAAATGAAGATAAGAGCATATCACTTCAAATAAGTCATGCTTTGGAGCTTCCACTACATTGGAGAATTTCTCGATGGGAGGCTCAATGGTTCATTAATATTTACGAAAGACAAGAAAATAAGAATCACACTTTACTTCAGTTTGCTAAATTGGATTACAACATTGTTCAAAACATCTACCAAGAAGACTTAAAGTATATGTCAAGGTAATAAAGTAGTCATTACGTTTCATGGACAACCAATTTCCCCTACACCTACGACAGTCGGTTAATCACATGTAAAATTGTGGTTAATTCATTTGACAAGATGGTTAATTTAAATCACATTTAAAATTGTGAATTTTGGTTAATTGAATTAACTTAAATTTGGTTATGAATTAAATTAATATTCAACTATATTAATCACATTTGTGAATGTGATTAATTAATTTGAGACCGCATCGTAGGTGTAGAAAAATAGGTGTCTCAATATTACCGTTACCGATAATATGTGCTCACGTAGTGGTGTCTAAATATTTGTCGAATGAACCATATACTATATATCCTGATTTTACAATAAATGATCTTCACATACTGCTTTTCTTTTGTATTTTATCTTGATTTCGTTGTCTAGTGTGGTTTTCTTTTGTTCATCGTCTTGGTATGTCGGTTTTCCATATCATTGCAATGTTCTAGACTGTAAGATCAATTTCAATCAATTATAAATTTAATCAATGGCTTAGACGTCAACATTAAGAATCTGTATTTCTTTACATATAAATGACTTTATGTAAATTTTCATGCTATCTTATCACAATTAGTGAATAAATTTTTTTTTTGTTCTTTTTAAGCTGGTGGAAAAGAATCGAACTTGGTGAAAAGTTGAGATTTTCCAGGGATAGAATTGTTGAAAATTTTGTTTGGACCGTGGGAACAAATTTCAAGTCTGATTTTGAAAATACCGAAAAGATATAACCAAGGTATATGCCTTGATTACCACAATTGATGATATTTATGATGTGCATGGTACCTTGGAAGAACTAGAGCTCTTCACCAAAGCAATTGATAGGTAATTGTTTTTTTGTTACAATATTGATGTTTAACAATAATTTATGTTAATTTATTTCCCTTATTCTCATTTAAAAGTAATTCTTGTTATTGAGTAATTATCAAAATTTGACTTGTGAATCTAGATGGGATCTAAATGCCATGGAACCTCTCCCACAATACATGAAAATATGCTTTCATGCACTCTATAACTATGTGAATGAAGTTGCATTTGAAACCTTGAAAAAAAGTGGGCACTATATCACTCCATACCTAAAGAAAGCAGTAAGTAATATTATATCTTTCATGATAACAATCTCTATTTATTATGAGTTTTTAGGCATATGATATATTATGATTTTATGTGTTTTTATAGTGGACAGATATATGTAATGCATATTTGATAGAAGCAAGGTGGTATCATAGTGGATACATTCCAAGTCTTGAAGAATACATAGAGAATGCATGGATATCCATAGGTGCACCAGATATACTTATCCATAGTTACTTTCTGATTCCACGTTCATTCAAAATGGAAGACTTGGTTAGTTTAGAAGAAAACTCAAACATGATTAGGCTTTCAGCAATGCTTTTACGCCTTGCTAATGACCTTGGATCTTATAAAGTAAGTTTCCAGCATAATTATTCCTTACAAAATTAATCTCTTCCTTCGTTATTTTTTTAAACGTTTCCTTCATTATTGTTATGATACATGACATCCACAAAAAATTTGAGAGAAAATTAAAAAAACTTTGTATTGACGATAATGAAGAATATTTGCAAATGAGGAAGTTAACCTTTCATGTCATGATCGTGTTCACAATATTTCCATCTTGGGACGAATAATGGAAAACCGCGTAAAAAAATTAATACAATGAGAATTGCTGGAGTACATCGGCTGAAAATAATTATACTTTAGAATCACCTTCTTCATACATACAATTTAACCTTTAACTTATCACTAATTGTTTTCCTTTTTCTTTTTTCAGCGTGAAAATGAGATGGGTGATAATCCAAAGATAATTCAATGCTACATGAACGATCGTGGAGCTTCTGAAGTCGAGGCTCGTGAGTATGTAAAGTCCTTGTTGTGTACAACATGGAAGAAGATGAATAAAGAAGCTCATACTTCATCTTTTTCTCAAAGTTTCATAGATACTGCTTTGAACATTGGTAGAATGGCATTGTTCATGTACCTACATGGAGATGGTCATAGCATTCAAGACCCTGAAATTCAAAATCGCATAATGTCATTAATTTTTCAGCCCTTTCCTATTCAATGTTGTGTTGAGTAA
Protein sequence

>MS.gene77594.t1

MRNFKKGQSVDVEGMLSMYEASFHSFENEIILDEARDLTSKFLKEYLDQNEDKSISLQISHALELPLHWRISRWEAQWFINIYERQENKNHTLLQFAKLDYNIVQNIYQEDLKYMSSWWKRIELGEKLRFSRDRIVENFVWTVGTNFKWDLNAMEPLPQYMKICFHALYNYVNEVAFETLKKSGHYITPYLKKAWTDICNAYLIEARWYHSGYIPSLEEYIENAWISIGAPDILIHSYFLIPRSFKMEDLVSLEENSNMIRLSAMLLRLANDLGSYKRENEMGDNPKIIQCYMNDRGASEVEAREYVKSLLCTTWKKMNKEAHTSSFSQSFIDTALNIGRMALFMYLHGDGHSIQDPEIQNRIMSLIFQPFPIQCCVE