AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066310.t1 MTR_2g064425 75.971 412 99 0 3 414 159 570 0.0 672
MS.gene066310.t1 MTR_2g064425 75.971 412 99 0 3 414 174 585 0.0 671
MS.gene066310.t1 MTR_2g065450 71.186 413 118 1 3 414 177 589 0.0 612
MS.gene066310.t1 MTR_8g057020 67.312 413 134 1 3 414 176 588 0.0 598
MS.gene066310.t1 MTR_2g064425 76.496 234 55 0 3 236 159 392 5.01e-131 384
MS.gene066310.t1 MTR_2g064425 76.496 234 55 0 3 236 174 407 8.20e-131 384
MS.gene066310.t1 MTR_4g045810 46.845 412 216 3 3 412 168 578 3.94e-128 382
MS.gene066310.t1 MTR_7g010710 38.293 410 246 5 9 413 146 553 1.46e-105 323
MS.gene066310.t1 MTR_2g089130 40.194 413 243 3 3 414 154 563 4.23e-102 315
MS.gene066310.t1 MTR_2g089120 38.889 414 248 4 3 414 154 564 1.14e-98 306
MS.gene066310.t1 MTR_4g081460 34.699 415 267 4 3 415 150 562 3.93e-82 263
MS.gene066310.t1 MTR_6g039440 33.012 415 274 4 3 415 141 553 3.94e-75 244
MS.gene066310.t1 MTR_4g048460 33.976 415 270 4 3 415 149 561 4.44e-73 239
MS.gene066310.t1 MTR_6g008560 32.289 415 277 4 3 415 145 557 2.20e-72 237
MS.gene066310.t1 MTR_5g094620 32.850 414 276 2 3 415 125 537 6.46e-72 235
MS.gene066310.t1 MTR_6g008560 31.762 403 271 4 15 415 1 401 6.29e-70 226
MS.gene066310.t1 MTR_4g092010 33.681 383 247 4 9 388 158 536 8.85e-70 231
MS.gene066310.t1 MTR_5g062230 31.884 414 280 2 3 415 141 553 2.06e-68 226
MS.gene066310.t1 MTR_2g082010 30.476 420 285 5 3 418 155 571 2.54e-68 227
MS.gene066310.t1 MTR_2g010960 32.353 408 265 5 2 403 106 508 7.18e-68 224
MS.gene066310.t1 MTR_3g063120 32.924 407 263 5 2 403 78 479 7.82e-68 223
MS.gene066310.t1 MTR_6g065040 32.512 406 262 7 4 403 2 401 1.57e-67 220
MS.gene066310.t1 MTR_6g064980 32.203 413 273 5 2 409 102 512 5.21e-67 222
MS.gene066310.t1 MTR_3g465090 31.618 408 267 7 2 403 102 503 6.62e-66 219
MS.gene066310.t1 MTR_5g073200 32.850 414 274 4 4 415 138 549 9.69e-66 219
MS.gene066310.t1 MTR_3g464190 31.643 414 281 2 3 415 145 557 2.27e-65 219
MS.gene066310.t1 MTR_4g092020 31.490 416 268 8 3 410 160 566 4.86e-65 218
MS.gene066310.t1 MTR_3g063170 31.618 408 267 7 2 403 102 503 7.32e-65 216
MS.gene066310.t1 MTR_3g052120 33.073 384 250 6 11 388 111 493 2.45e-64 215
MS.gene066310.t1 MTR_3g063140 31.785 409 265 7 2 403 102 503 8.77e-64 213
MS.gene066310.t1 MTR_2g081980 29.440 411 286 3 9 416 149 558 9.27e-64 214
MS.gene066310.t1 MTR_8g007515 30.404 421 286 5 1 416 139 557 2.70e-63 213
MS.gene066310.t1 MTR_4g048460 37.658 316 194 3 3 317 149 462 9.03e-61 204
MS.gene066310.t1 MTR_5g073260 29.880 415 287 4 3 415 140 552 6.74e-58 199
MS.gene066310.t1 MTR_2g082060 28.910 422 280 7 3 418 149 556 2.18e-57 197
MS.gene066310.t1 MTR_2g082050 27.053 414 262 6 3 412 79 456 1.15e-50 177
MS.gene066310.t1 MTR_4g019550 28.169 426 276 7 3 415 150 558 6.76e-49 174
MS.gene066310.t1 MTR_2g012870 28.267 329 212 6 14 325 343 664 3.40e-30 123
MS.gene066310.t1 MTR_2g012900 30.205 341 199 12 14 325 348 678 4.26e-30 123
MS.gene066310.t1 MTR_7g050990 40.972 144 76 1 61 204 29 163 9.43e-29 119
MS.gene066310.t1 MTR_3g058160 25.798 376 248 10 3 353 330 699 3.73e-27 114
MS.gene066310.t1 MTR_3g058160 25.798 376 248 10 3 353 343 712 3.74e-27 114
MS.gene066310.t1 MTR_3g058160 25.798 376 248 10 3 353 139 508 3.85e-27 114
MS.gene066310.t1 MTR_3g058160 25.798 376 248 10 3 353 350 719 4.28e-27 114
MS.gene066310.t1 MTR_3g058160 28.030 264 184 5 93 353 480 740 3.37e-26 111
MS.gene066310.t1 MTR_2g064295 24.277 346 234 8 3 325 330 670 3.81e-25 108
MS.gene066310.t1 MTR_5g094530 28.708 209 114 2 28 235 4 178 5.63e-23 95.9
MS.gene066310.t1 MTR_3g063160 29.787 188 130 2 2 188 102 288 2.00e-22 97.1
MS.gene066310.t1 MTR_5g081730 48.980 98 45 2 21 116 16 110 3.24e-19 84.3
MS.gene066310.t1 MTR_7g057340 23.837 172 130 1 245 415 1 172 3.46e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066310.t1 AT3G25830 44.686 414 220 5 11 415 181 594 1.66e-126 378
MS.gene066310.t1 AT3G25820 44.686 414 220 5 11 415 181 594 1.66e-126 378
MS.gene066310.t1 AT3G25810 44.391 419 222 6 8 415 179 597 4.42e-126 377
MS.gene066310.t1 AT4G16740 42.927 410 226 5 11 415 158 564 8.21e-122 365
MS.gene066310.t1 AT4G16730 41.647 425 233 5 4 415 71 493 1.15e-121 362
MS.gene066310.t1 AT4G16730 41.647 425 233 5 4 415 117 539 3.48e-121 363
MS.gene066310.t1 AT2G24210 43.187 433 222 11 1 415 164 590 1.39e-118 358
MS.gene066310.t1 AT3G25820 44.939 247 133 2 11 254 181 427 2.57e-73 236
MS.gene066310.t1 AT1G61680 31.841 402 267 5 3 398 162 562 3.49e-71 234
MS.gene066310.t1 AT1G61680 31.841 402 267 5 3 398 155 555 4.02e-71 234
MS.gene066310.t1 AT4G16740 45.217 230 122 2 11 236 158 387 2.60e-66 217
MS.gene066310.t1 AT5G23960 30.361 415 284 4 3 415 134 545 1.02e-62 211
MS.gene066310.t1 AT5G23960 30.456 417 283 5 3 415 134 547 2.34e-61 208
MS.gene066310.t1 AT1G70080 30.097 412 282 3 3 408 193 604 2.26e-54 191
MS.gene066310.t1 AT3G14520 26.619 417 301 2 4 415 188 604 2.07e-53 188
MS.gene066310.t1 AT1G31950 26.619 417 301 2 4 415 191 607 1.35e-52 186
MS.gene066310.t1 AT1G31950 26.619 417 301 2 4 415 190 606 1.81e-52 185
MS.gene066310.t1 AT3G14540 27.896 423 288 5 4 415 185 601 4.24e-51 181
MS.gene066310.t1 AT3G25820 45.355 183 97 2 11 190 181 363 3.43e-49 172
MS.gene066310.t1 AT3G25820 45.355 183 97 2 11 190 181 363 3.43e-49 172
MS.gene066310.t1 AT1G33750 27.791 421 289 5 3 415 190 603 1.52e-48 174
MS.gene066310.t1 AT4G16740 42.439 205 114 2 11 211 158 362 2.15e-48 169
MS.gene066310.t1 AT4G16740 42.439 205 114 2 11 211 158 362 2.15e-48 169
MS.gene066310.t1 AT3G29410 26.969 419 296 4 3 415 84 498 2.56e-48 172
MS.gene066310.t1 AT3G29410 26.969 419 296 4 3 415 189 603 1.41e-47 172
MS.gene066310.t1 AT3G14490 27.619 420 292 7 4 415 186 601 3.32e-47 171
MS.gene066310.t1 AT3G29190 28.469 418 293 5 3 415 185 601 1.12e-46 169
MS.gene066310.t1 AT3G32030 25.542 415 293 6 11 415 196 604 2.84e-43 160
MS.gene066310.t1 AT3G29110 28.362 409 289 4 4 408 182 590 3.53e-43 159
MS.gene066310.t1 AT1G31950 27.273 319 229 1 4 319 191 509 4.50e-42 155
MS.gene066310.t1 AT3G14540 29.231 325 215 4 4 319 185 503 2.60e-41 153
MS.gene066310.t1 AT4G15870 25.366 410 290 6 3 408 194 591 2.39e-40 152
MS.gene066310.t1 AT4G20230 25.000 412 305 4 3 410 188 599 3.64e-39 149
MS.gene066310.t1 AT4G20230 25.000 412 305 4 3 410 200 611 4.21e-39 149
MS.gene066310.t1 AT5G44630 27.470 415 293 6 4 412 142 554 5.46e-39 147
MS.gene066310.t1 AT5G48110 28.481 316 223 3 79 393 5 318 1.50e-38 142
MS.gene066310.t1 AT2G23230 27.136 398 280 6 4 394 185 579 4.88e-36 140
MS.gene066310.t1 AT2G23230 26.396 394 283 4 4 394 185 574 7.07e-36 139
MS.gene066310.t1 AT4G20210 26.429 420 299 7 3 415 184 600 1.40e-35 138
MS.gene066310.t1 AT1G48820 28.767 365 239 6 3 363 163 510 2.44e-35 137
MS.gene066310.t1 AT4G20200 24.390 410 306 4 3 408 188 597 2.56e-35 137
MS.gene066310.t1 AT1G48800 27.553 421 278 10 4 408 190 599 2.86e-35 137
MS.gene066310.t1 AT1G66020 24.878 410 304 4 3 408 182 591 5.44e-35 137
MS.gene066310.t1 AT4G13300 24.638 414 308 4 3 412 138 551 4.65e-33 130
MS.gene066310.t1 AT4G13280 24.396 414 309 4 3 412 138 551 1.08e-32 130
MS.gene066310.t1 AT1G79460 26.444 329 233 6 93 416 456 780 6.38e-29 120
MS.gene066310.t1 AT1G66020 30.144 209 143 3 3 208 182 390 1.05e-24 105
MS.gene066310.t1 AT4G13280 22.947 414 285 6 3 412 138 521 6.13e-24 104
MS.gene066310.t1 AT1G61120 27.429 350 216 9 15 344 379 710 4.25e-22 99.8

Find 61 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACTTCAGTTTGCTAAAT+TGG 0.103435 2.3:+30334450 MS.gene066310:CDS
AAAGTTTCTCAAAGAATATT+TGG 0.167285 2.3:+30334294 MS.gene066310:CDS
ATATGCTCTTATCTTCATTT+TGG 0.204420 2.3:-30334318 None:intergenic
GTTTGAAAATGAAATTATAT+TGG 0.247458 2.3:+30334249 MS.gene066310:CDS
GATTCCACGTTCATTCAAAA+TGG 0.264512 2.3:+30335857 MS.gene066310:CDS
ACATATACTTTAAGTCTTCT+TGG 0.268413 2.3:-30334498 None:intergenic
TATTACTTACTGCTTTCTTT+AGG 0.304141 2.3:-30335616 None:intergenic
ATGTAGCATTGAATTATCTT+TGG 0.305826 2.3:-30336358 None:intergenic
TAACTATGGATAAGTATATC+TGG 0.362564 2.3:-30335828 None:intergenic
CAATTACCTATCAATTGCTT+TGG 0.379210 2.3:-30335350 None:intergenic
CTTACTGCTTTCTTTAGGTA+TGG 0.393417 2.3:-30335611 None:intergenic
ACTTCAAATAAGTCATGCTT+TGG 0.406823 2.3:+30334342 MS.gene066310:CDS
AAGAAAACTCAAACATGATT+AGG 0.407501 2.3:+30335898 MS.gene066310:CDS
TACTCAACACAACATTGAAT+AGG 0.408865 2.3:-30336616 None:intergenic
GTGGAATCAGAAAGTAACTA+TGG 0.412857 2.3:-30335842 None:intergenic
ATATTCTTTGAGAAACTTTG+AGG 0.413496 2.3:-30334290 None:intergenic
TGCTTTGAACATTGGTAGAA+TGG 0.422510 2.3:+30336504 MS.gene066310:CDS
TCTTCACCAAAGCAATTGAT+AGG 0.429846 2.3:+30335344 MS.gene066310:CDS
AAGTATATCTGGTGCACCTA+TGG 0.431316 2.3:-30335817 None:intergenic
ATCATCAATTGTGGTAATCA+AGG 0.434604 2.3:-30335284 None:intergenic
TTACGCCTTGCTAATGACCT+TGG 0.465962 2.3:+30335936 MS.gene066310:CDS
CTTTGGAGCTTCCACTACAT+TGG 0.473873 2.3:+30334359 MS.gene066310:CDS
TTTCTCGATGGGAGGCTCAA+TGG 0.484973 2.3:+30334386 MS.gene066310:CDS
TCTATGTATTCTTCAAGACT+TGG 0.487482 2.3:-30335780 None:intergenic
ATAGATACTGCTTTGAACAT+TGG 0.501453 2.3:+30336496 MS.gene066310:CDS
TTCATTCAAAATGGAAGACT+TGG 0.509358 2.3:+30335866 MS.gene066310:CDS
AACACAACATTGAATAGGAA+AGG 0.527983 2.3:-30336611 None:intergenic
GGCCAATCTGTTGATGTTGA+GGG 0.533101 2.3:+30334193 MS.gene066310:CDS
TTCCCTCAACATCAACAGAT+TGG 0.534301 2.3:-30334195 None:intergenic
AGGCCAATCTGTTGATGTTG+AGG 0.541057 2.3:+30334192 MS.gene066310:CDS
AAGAATACATAGAGAATGCA+TGG 0.541704 2.3:+30335790 MS.gene066310:CDS
TTTCAGCGTGAAAATGAGAT+GGG 0.542662 2.3:+30336328 MS.gene066310:intron
ACACAACATTGAATAGGAAA+GGG 0.549956 2.3:-30336610 None:intergenic
AGACTTGAAATTTGTTCCCA+CGG 0.551562 2.3:-30335221 None:intergenic
CGAGAAATTCTCCAATGTAG+TGG 0.558830 2.3:-30334370 None:intergenic
TTATGATGTGCATGGTACCT+TGG 0.560316 2.3:+30335309 MS.gene066310:CDS
ATCATAAATATCATCAATTG+TGG 0.568920 2.3:-30335293 None:intergenic
TACATTGGAGAATTTCTCGA+TGG 0.572320 2.3:+30334374 MS.gene066310:CDS
CTTCCATGTTGTACACAACA+AGG 0.573406 2.3:-30336428 None:intergenic
TAGATGGGATCTAAATGCCA+TGG 0.574694 2.3:+30335487 MS.gene066310:intron
GCATTGTTCATGTACCTACA+TGG 0.575275 2.3:+30336526 MS.gene066310:CDS
GTATTGTGGGAGAGGTTCCA+TGG 0.575761 2.3:-30335504 None:intergenic
GGTAATCAAGGCATATACCT+TGG 0.581141 2.3:-30335272 None:intergenic
GAGAATGCATGGATATCCAT+AGG 0.583758 2.3:+30335801 MS.gene066310:CDS
AACTTACTTTATAAGATCCA+AGG 0.584705 2.3:-30335953 None:intergenic
TCGTGGAGCTTCTGAAGTCG+AGG 0.598317 2.3:+30336387 MS.gene066310:CDS
CAATGCTACATGAACGATCG+TGG 0.605163 2.3:+30336370 MS.gene066310:CDS
GAAGAGCTCGAGTTCTTCCA+AGG 0.605936 2.3:-30335326 None:intergenic
TGATATGACCATCTCCATGT+AGG 0.609569 2.3:-30336540 None:intergenic
AAGATCCAAGGTCATTAGCA+AGG 0.609710 2.3:-30335941 None:intergenic
TGGTGGAAAAGAATCGAACT+TGG 0.611617 2.3:+30335148 MS.gene066310:CDS
GAAGCAAGGTGGTATCATAG+TGG 0.612090 2.3:+30335750 MS.gene066310:CDS
TTCATGTACCTACATGGAGA+TGG 0.612512 2.3:+30336532 MS.gene066310:CDS
TTGGAGAATTTCTCGATGGG+AGG 0.615953 2.3:+30334378 MS.gene066310:CDS
AAGACTTAAAGTATATGTCA+AGG 0.616611 2.3:+30334503 MS.gene066310:CDS
ACATTGGAGAATTTCTCGAT+GGG 0.625313 2.3:+30334375 MS.gene066310:CDS
ATGCATATTTGATAGAAGCA+AGG 0.628575 2.3:+30335736 MS.gene066310:CDS
GATGATATTTATGATGTGCA+TGG 0.632583 2.3:+30335301 MS.gene066310:CDS
AGTCCTTGTTGTGTACAACA+TGG 0.651967 2.3:+30336425 MS.gene066310:CDS
CATATTTGATAGAAGCAAGG+TGG 0.695260 2.3:+30335739 MS.gene066310:CDS
TTACCGAAAAGATATAACCA+AGG 0.713401 2.3:+30335255 MS.gene066310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTGAATTAACTTAAATT+TGG + chr2.3:30334665-30334684 MS.gene066310:intron 10.0%
!! GTTTGAAAATGAAATTATAT+TGG + chr2.3:30334249-30334268 MS.gene066310:CDS 15.0%
!! TCAATTATAAATTCAATCAA+TGG + chr2.3:30335012-30335031 MS.gene066310:intron 15.0%
!!! ACATTTAAAATTGTGAATTT+TGG + chr2.3:30334641-30334660 MS.gene066310:intron 15.0%
!!! ATTACTTTTAAATGAGAATA+AGG - chr2.3:30335427-30335446 None:intergenic 15.0%
!!! ATTGTATTAATTTTTTTACG+CGG - chr2.3:30336201-30336220 None:intergenic 15.0%
!!! TCTATTTATTATGAGTTTTT+AGG + chr2.3:30335662-30335681 MS.gene066310:intron 15.0%
!!! TTACTTTTAAATGAGAATAA+GGG - chr2.3:30335426-30335445 None:intergenic 15.0%
!! AAAGTTTCTCAAAGAATATT+TGG + chr2.3:30334294-30334313 MS.gene066310:CDS 20.0%
!! AAATCAGGATATATAGTATA+TGG - chr2.3:30334849-30334868 None:intergenic 20.0%
!! AACAATTAGTGATAAGTTAA+AGG - chr2.3:30336295-30336314 None:intergenic 20.0%
!! AACGTTTAAAAAAATAACGA+AGG - chr2.3:30336014-30336033 None:intergenic 20.0%
!! AGATCATTTATTGTAAAATC+AGG - chr2.3:30334864-30334883 None:intergenic 20.0%
!! ATCATAAATATCATCAATTG+TGG - chr2.3:30335296-30335315 None:intergenic 20.0%
!! ATGTATCATAACAATAATGA+AGG - chr2.3:30336038-30336057 None:intergenic 20.0%
!! TTAAATTGTATGTATGAAGA+AGG - chr2.3:30336272-30336291 None:intergenic 20.0%
!!! GAATTGTTGAAAATTTTGTT+TGG + chr2.3:30335197-30335216 MS.gene066310:CDS 20.0%
!!! GATTTTATGTGTTTTTATAG+TGG + chr2.3:30335700-30335719 MS.gene066310:intron 20.0%
!!! TTTTCTCTCAAATTTTTTTG+TGG - chr2.3:30336066-30336085 None:intergenic 20.0%
!!! TTTTTTTGTTCTTTTTAAGC+TGG + chr2.3:30335128-30335147 MS.gene066310:intron 20.0%
! AACTTACTTTATAAGATCCA+AGG - chr2.3:30335956-30335975 None:intergenic 25.0%
! AAGAAAACTCAAACATGATT+AGG + chr2.3:30335898-30335917 MS.gene066310:CDS 25.0%
! AAGACTTAAAGTATATGTCA+AGG + chr2.3:30334503-30334522 MS.gene066310:CDS 25.0%
! ACATATACTTTAAGTCTTCT+TGG - chr2.3:30334501-30334520 None:intergenic 25.0%
! ATGAAGAATATTTGCAAATG+AGG + chr2.3:30336111-30336130 MS.gene066310:intron 25.0%
! ATTAATACAATGAGAATTGC+TGG + chr2.3:30336210-30336229 MS.gene066310:intron 25.0%
! GTTAATCACATGTAAAATTG+TGG + chr2.3:30334585-30334604 MS.gene066310:intron 25.0%
! TAACTATGGATAAGTATATC+TGG - chr2.3:30335831-30335850 None:intergenic 25.0%
! TATTACTTACTGCTTTCTTT+AGG - chr2.3:30335619-30335638 None:intergenic 25.0%
! TTGAAACCTTGAAAAAAAGT+GGG + chr2.3:30335576-30335595 MS.gene066310:CDS 25.0%
! TTTGTAAGGAATAATTATGC+TGG - chr2.3:30335980-30335999 None:intergenic 25.0%
!! AAGCATATTTTCATGTATTG+TGG - chr2.3:30335521-30335540 None:intergenic 25.0%
!! AGCATATTTTCATGTATTGT+GGG - chr2.3:30335520-30335539 None:intergenic 25.0%
!! AGGAAGAGATTAATTTTGTA+AGG - chr2.3:30335994-30336013 None:intergenic 25.0%
!! ATACCTTGGTTATATCTTTT+CGG - chr2.3:30335261-30335280 None:intergenic 25.0%
!! ATATGCTCTTATCTTCATTT+TGG - chr2.3:30334321-30334340 None:intergenic 25.0%
!! ATATTCTTTGAGAAACTTTG+AGG - chr2.3:30334293-30334312 None:intergenic 25.0%
!! ATGTAGCATTGAATTATCTT+TGG - chr2.3:30336361-30336380 None:intergenic 25.0%
!! ATTATGCGATTTTGAATTTC+AGG - chr2.3:30336574-30336593 None:intergenic 25.0%
!! TTATGCGATTTTGAATTTCA+GGG - chr2.3:30336573-30336592 None:intergenic 25.0%
!! TTTGAAACCTTGAAAAAAAG+TGG + chr2.3:30335575-30335594 MS.gene066310:CDS 25.0%
!!! TTTACTTCAGTTTGCTAAAT+TGG + chr2.3:30334450-30334469 MS.gene066310:CDS 25.0%
AACACAACATTGAATAGGAA+AGG - chr2.3:30336614-30336633 None:intergenic 30.0%
AAGAATACATAGAGAATGCA+TGG + chr2.3:30335790-30335809 MS.gene066310:CDS 30.0%
ACACAACATTGAATAGGAAA+GGG - chr2.3:30336613-30336632 None:intergenic 30.0%
ACTTCAAATAAGTCATGCTT+TGG + chr2.3:30334342-30334361 MS.gene066310:CDS 30.0%
ATCATCAATTGTGGTAATCA+AGG - chr2.3:30335287-30335306 None:intergenic 30.0%
ATGCATATTTGATAGAAGCA+AGG + chr2.3:30335736-30335755 MS.gene066310:CDS 30.0%
CAATTACCTATCAATTGCTT+TGG - chr2.3:30335353-30335372 None:intergenic 30.0%
GATGATATTTATGATGTGCA+TGG + chr2.3:30335301-30335320 MS.gene066310:CDS 30.0%
GGTTAATTCATTTGACAAGA+TGG + chr2.3:30334606-30334625 MS.gene066310:intron 30.0%
TACTCAACACAACATTGAAT+AGG - chr2.3:30336619-30336638 None:intergenic 30.0%
TCTATGTATTCTTCAAGACT+TGG - chr2.3:30335783-30335802 None:intergenic 30.0%
TTACCGAAAAGATATAACCA+AGG + chr2.3:30335255-30335274 MS.gene066310:CDS 30.0%
TTCACGCTGAAAAAAGAAAA+AGG - chr2.3:30336320-30336339 None:intergenic 30.0%
TTCATTCAAAATGGAAGACT+TGG + chr2.3:30335866-30335885 MS.gene066310:CDS 30.0%
! ATAGATACTGCTTTGAACAT+TGG + chr2.3:30336496-30336515 MS.gene066310:CDS 30.0%
! ATTTTCAACAATTCTATCCC+TGG - chr2.3:30335191-30335210 None:intergenic 30.0%
! TCTAGAACATTGCAATGATA+TGG - chr2.3:30334974-30334993 None:intergenic 30.0%
! TGAAAAGTTGAGATTTTCCA+GGG + chr2.3:30335171-30335190 MS.gene066310:CDS 30.0%
! TTTTCTTTTGTTCATCGTCT+TGG + chr2.3:30334936-30334955 MS.gene066310:intron 30.0%
!! ATTTGACTTGTGAATCTAGA+TGG + chr2.3:30335471-30335490 MS.gene066310:intron 30.0%
!! TTTGACTTGTGAATCTAGAT+GGG + chr2.3:30335472-30335491 MS.gene066310:intron 30.0%
!! TTTTGTTCTTTTTAAGCTGG+TGG + chr2.3:30335131-30335150 MS.gene066310:intron 30.0%
AAAGCAGTCATTACGTTTCA+TGG + chr2.3:30334530-30334549 MS.gene066310:intron 35.0%
ACATTGGAGAATTTCTCGAT+GGG + chr2.3:30334375-30334394 MS.gene066310:CDS 35.0%
ACTACGTGAGCACATATTAT+CGG - chr2.3:30334801-30334820 None:intergenic 35.0%
CATATTTGATAGAAGCAAGG+TGG + chr2.3:30335739-30335758 MS.gene066310:CDS 35.0%
CATCGTAGGTGTAGAAAAAT+AGG + chr2.3:30334756-30334775 MS.gene066310:intron 35.0%
CTTACTGCTTTCTTTAGGTA+TGG - chr2.3:30335614-30335633 None:intergenic 35.0%
GATTCCACGTTCATTCAAAA+TGG + chr2.3:30335857-30335876 MS.gene066310:CDS 35.0%
GTGGAATCAGAAAGTAACTA+TGG - chr2.3:30335845-30335864 None:intergenic 35.0%
GTTCACAATACTTCCATCTT+GGG + chr2.3:30336160-30336179 MS.gene066310:intron 35.0%
TACATTGGAGAATTTCTCGA+TGG + chr2.3:30334374-30334393 MS.gene066310:CDS 35.0%
TCTTCACCAAAGCAATTGAT+AGG + chr2.3:30335344-30335363 MS.gene066310:CDS 35.0%
TGAGCACATATTATCGGTAA+CGG - chr2.3:30334795-30334814 None:intergenic 35.0%
TGTTCACAATACTTCCATCT+TGG + chr2.3:30336159-30336178 MS.gene066310:intron 35.0%
TTTCAGCGTGAAAATGAGAT+GGG + chr2.3:30336328-30336347 MS.gene066310:intron 35.0%
! AGACTTGAAATTTGTTCCCA+CGG - chr2.3:30335224-30335243 None:intergenic 35.0%
! ATTTTCATGTATTGTGGGAG+AGG - chr2.3:30335515-30335534 None:intergenic 35.0%
! ATTTTTCTACACCTACGATG+CGG - chr2.3:30334756-30334775 None:intergenic 35.0%
! GTGAAAAGTTGAGATTTTCC+AGG + chr2.3:30335170-30335189 MS.gene066310:CDS 35.0%
! TCTTCCATTTTGAATGAACG+TGG - chr2.3:30335864-30335883 None:intergenic 35.0%
! TGCTTTGAACATTGGTAGAA+TGG + chr2.3:30336504-30336523 MS.gene066310:CDS 35.0%
! TTTTCAGCGTGAAAATGAGA+TGG + chr2.3:30336327-30336346 MS.gene066310:intron 35.0%
!! GAAAATTTTGTTTGGACCGT+GGG + chr2.3:30335205-30335224 MS.gene066310:CDS 35.0%
!! TGAAAATTTTGTTTGGACCG+TGG + chr2.3:30335204-30335223 MS.gene066310:CDS 35.0%
!!! ATAGTGCCCACTTTTTTTCA+AGG - chr2.3:30335585-30335604 None:intergenic 35.0%
AAGATCCAAGGTCATTAGCA+AGG - chr2.3:30335944-30335963 None:intergenic 40.0%
AAGTATATCTGGTGCACCTA+TGG - chr2.3:30335820-30335839 None:intergenic 40.0%
CGAGAAATTCTCCAATGTAG+TGG - chr2.3:30334373-30334392 None:intergenic 40.0%
CTTCCATGTTGTACACAACA+AGG - chr2.3:30336431-30336450 None:intergenic 40.0%
CTTGATTTCGTTGTCTAGTG+TGG + chr2.3:30334913-30334932 MS.gene066310:intron 40.0%
GAACACGATCATGACATGAA+AGG - chr2.3:30336144-30336163 None:intergenic 40.0%
GAGAATGCATGGATATCCAT+AGG + chr2.3:30335801-30335820 MS.gene066310:CDS 40.0%
GATAATATGTGCTCACGTAG+TGG + chr2.3:30334800-30334819 MS.gene066310:intron 40.0%
GCATTGTTCATGTACCTACA+TGG + chr2.3:30336526-30336545 MS.gene066310:CDS 40.0%
GGTAATCAAGGCATATACCT+TGG - chr2.3:30335275-30335294 None:intergenic 40.0%
TAGATGGGATCTAAATGCCA+TGG + chr2.3:30335487-30335506 MS.gene066310:intron 40.0%
TGAGAATTGCTGGAGTACAT+CGG + chr2.3:30336220-30336239 MS.gene066310:intron 40.0%
TGATATGACCATCTCCATGT+AGG - chr2.3:30336543-30336562 None:intergenic 40.0%
TGGTGGAAAAGAATCGAACT+TGG + chr2.3:30335148-30335167 MS.gene066310:CDS 40.0%
TGTTCATCGTCTTGGTATGT+CGG + chr2.3:30334944-30334963 MS.gene066310:intron 40.0%
TTAATTTCAGACCGCATCGT+AGG + chr2.3:30334742-30334761 MS.gene066310:intron 40.0%
TTATGATGTGCATGGTACCT+TGG + chr2.3:30335309-30335328 MS.gene066310:CDS 40.0%
TTCATGTACCTACATGGAGA+TGG + chr2.3:30336532-30336551 MS.gene066310:CDS 40.0%
TTCCATCTTGGGACGAATAA+TGG + chr2.3:30336171-30336190 MS.gene066310:intron 40.0%
TTCCATTATTCGTCCCAAGA+TGG - chr2.3:30336176-30336195 None:intergenic 40.0%
TTCCCTCAACATCAACAGAT+TGG - chr2.3:30334198-30334217 None:intergenic 40.0%
! AGTCCTTGTTGTGTACAACA+TGG + chr2.3:30336425-30336444 MS.gene066310:CDS 40.0%
AGGCCAATCTGTTGATGTTG+AGG + chr2.3:30334192-30334211 MS.gene066310:CDS 45.0%
CAATGCTACATGAACGATCG+TGG + chr2.3:30336370-30336389 MS.gene066310:CDS 45.0%
GAAGCAAGGTGGTATCATAG+TGG + chr2.3:30335750-30335769 MS.gene066310:CDS 45.0%
GGCCAATCTGTTGATGTTGA+GGG + chr2.3:30334193-30334212 MS.gene066310:CDS 45.0%
TGTGATTAACCGACTGTCGT+AGG - chr2.3:30334575-30334594 None:intergenic 45.0%
TTACGCCTTGCTAATGACCT+TGG + chr2.3:30335936-30335955 MS.gene066310:CDS 45.0%
TTGGAGAATTTCTCGATGGG+AGG + chr2.3:30334378-30334397 MS.gene066310:CDS 45.0%
!! CTTTGGAGCTTCCACTACAT+TGG + chr2.3:30334359-30334378 MS.gene066310:CDS 45.0%
AACCGACTGTCGTAGGTGTA+GGG - chr2.3:30334568-30334587 None:intergenic 50.0%
GAAGAGCTCGAGTTCTTCCA+AGG - chr2.3:30335329-30335348 None:intergenic 50.0%
GTATTGTGGGAGAGGTTCCA+TGG - chr2.3:30335507-30335526 None:intergenic 50.0%
GTCGTAGGTGTAGGGGAAAT+TGG - chr2.3:30334560-30334579 None:intergenic 50.0%
TAACCGACTGTCGTAGGTGT+AGG - chr2.3:30334569-30334588 None:intergenic 50.0%
TTTCTCGATGGGAGGCTCAA+TGG + chr2.3:30334386-30334405 MS.gene066310:CDS 50.0%
ACCGACTGTCGTAGGTGTAG+GGG - chr2.3:30334567-30334586 None:intergenic 55.0%
TCCCCTACACCTACGACAGT+CGG + chr2.3:30334563-30334582 MS.gene066310:intron 55.0%
!! TCGTGGAGCTTCTGAAGTCG+AGG + chr2.3:30336387-30336406 MS.gene066310:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 30334175 30336639 30334175 ID=MS.gene066310
chr2.3 mRNA 30334175 30336639 30334175 ID=MS.gene066310.t1;Parent=MS.gene066310
chr2.3 exon 30334175 30334524 30334175 ID=MS.gene066310.t1.exon1;Parent=MS.gene066310.t1
chr2.3 CDS 30334175 30334524 30334175 ID=cds.MS.gene066310.t1;Parent=MS.gene066310.t1
chr2.3 exon 30335147 30335365 30335147 ID=MS.gene066310.t1.exon2;Parent=MS.gene066310.t1
chr2.3 CDS 30335147 30335365 30335147 ID=cds.MS.gene066310.t1;Parent=MS.gene066310.t1
chr2.3 exon 30335490 30335628 30335490 ID=MS.gene066310.t1.exon3;Parent=MS.gene066310.t1
chr2.3 CDS 30335490 30335628 30335490 ID=cds.MS.gene066310.t1;Parent=MS.gene066310.t1
chr2.3 exon 30335720 30335968 30335720 ID=MS.gene066310.t1.exon4;Parent=MS.gene066310.t1
chr2.3 CDS 30335720 30335968 30335720 ID=cds.MS.gene066310.t1;Parent=MS.gene066310.t1
chr2.3 exon 30336334 30336639 30336334 ID=MS.gene066310.t1.exon5;Parent=MS.gene066310.t1
chr2.3 CDS 30336334 30336639 30336334 ID=cds.MS.gene066310.t1;Parent=MS.gene066310.t1
Gene Sequence

>MS.gene066310

ATGCGTAATTTTAAGAAAGGCCAATCTGTTGATGTTGAGGGAATGTTATCGATGTATGAAGCATCATTTCATTCGTTTGAAAATGAAATTATATTGGATGAAGCAAGAGATTTGACCTCAAAGTTTCTCAAAGAATATTTGGACCAAAATGAAGATAAGAGCATATCACTTCAAATAAGTCATGCTTTGGAGCTTCCACTACATTGGAGAATTTCTCGATGGGAGGCTCAATGGTTCATTAATATTTACGAAAGACAAGAAAATAAGAATCACACTTTACTTCAGTTTGCTAAATTGGATTACAACATTGTTCAAAACATCTACCAAGAAGACTTAAAGTATATGTCAAGGTAATAAAGCAGTCATTACGTTTCATGGACAACCAATTTCCCCTACACCTACGACAGTCGGTTAATCACATGTAAAATTGTGGTTAATTCATTTGACAAGATGGTTAATTTAAATCACATTTAAAATTGTGAATTTTGGTTAATTGAATTAACTTAAATTTGGTTATGAATTAAATTAATATTCAACTATATTAATCACATTTGTGAATGTGATTAATTAATTTCAGACCGCATCGTAGGTGTAGAAAAATAGGTGTCTCAGTATTACCGTTACCGATAATATGTGCTCACGTAGTGGTGTCTAAATATTTGTCGAATGAACCATATACTATATATCCTGATTTTACAATAAATGATCTTCACATACTGCTTTTCTTTTGTATTTTATCTTGATTTCGTTGTCTAGTGTGGTTTTCTTTTGTTCATCGTCTTGGTATGTCGGTTTTCCATATCATTGCAATGTTCTAGACTGTAAGATCAATTTCAATCAATTATAAATTCAATCAATGGCTTAGACGTCAACATTAAGAATCTGTATTTCTTTACATATAAATGACTTTATGTAAATTTTCATGCTATCTTATCACAATTAGTGAATAAATTTTTTTTTGTTCTTTTTAAGCTGGTGGAAAAGAATCGAACTTGGTGAAAAGTTGAGATTTTCCAGGGATAGAATTGTTGAAAATTTTGTTTGGACCGTGGGAACAAATTTCAAGTCTGATTTTGAAAATTACCGAAAAGATATAACCAAGGTATATGCCTTGATTACCACAATTGATGATATTTATGATGTGCATGGTACCTTGGAAGAACTCGAGCTCTTCACCAAAGCAATTGATAGGTAATTGTTTTTTTGTTACAATATTGATGTTTAACAATAATTTATGTTAATTTATTTCCCTTATTCTCATTTAAAAGTAATTCTTGTTATTGAGTAATTATCAAAATTTGACTTGTGAATCTAGATGGGATCTAAATGCCATGGAACCTCTCCCACAATACATGAAAATATGCTTTCATGCACTCTATAACTATGTGAATGAAGTTGCATTTGAAACCTTGAAAAAAAGTGGGCACTATATCACACCATACCTAAAGAAAGCAGTAAGTAATATTATATCTTTCATGATAACAATCTCTATTTATTATGAGTTTTTAGGCATATGATATATTATGATTTTATGTGTTTTTATAGTGGACAGATATATGTAATGCATATTTGATAGAAGCAAGGTGGTATCATAGTGGATACATTCCAAGTCTTGAAGAATACATAGAGAATGCATGGATATCCATAGGTGCACCAGATATACTTATCCATAGTTACTTTCTGATTCCACGTTCATTCAAAATGGAAGACTTGGTTAGTTTAGAAGAAAACTCAAACATGATTAGGCTTTCAGCAATGCTTTTACGCCTTGCTAATGACCTTGGATCTTATAAAGTAAGTTTCCAGCATAATTATTCCTTACAAAATTAATCTCTTCCTTCGTTATTTTTTTAAACGTTTCCTTCATTATTGTTATGATACATGACATCCACAAAAAAATTTGAGAGAAAATTAAAAAAACTTTGTATTGACGATAATGAAGAATATTTGCAAATGAGGAAGTTAACCTTTCATGTCATGATCGTGTTCACAATACTTCCATCTTGGGACGAATAATGGAAAACCGCGTAAAAAAATTAATACAATGAGAATTGCTGGAGTACATCGGCTGAAAATAATTATACTTTAGAATCACCTTCTTCATACATACAATTTAACCTTTAACTTATCACTAATTGTTTTCCTTTTTCTTTTTTCAGCGTGAAAATGAGATGGGTGATAATCCAAAGATAATTCAATGCTACATGAACGATCGTGGAGCTTCTGAAGTCGAGGCTCGTGAGTATGTAAAGTCCTTGTTGTGTACAACATGGAAGAAGATGAATAAAGAAGCTCATACTTCATCTTTTTCTCAAAGTTTCATAGATACTGCTTTGAACATTGGTAGAATGGCATTGTTCATGTACCTACATGGAGATGGTCATATCATTCAAGACCCTGAAATTCAAAATCGCATAATGTCATTAATTTTTCAGCCCTTTCCTATTCAATGTTGTGTTGAGTAA
Protein sequence

>MS.gene066310.t1

MRNFKKGQSVDVEGMLSMYEASFHSFENEIILDEARDLTSKFLKEYLDQNEDKSISLQISHALELPLHWRISRWEAQWFINIYERQENKNHTLLQFAKLDYNIVQNIYQEDLKYMSSWWKRIELGEKLRFSRDRIVENFVWTVGTNFKSDFENYRKDITKVYALITTIDDIYDVHGTLEELELFTKAIDRWDLNAMEPLPQYMKICFHALYNYVNEVAFETLKKSGHYITPYLKKAWTDICNAYLIEARWYHSGYIPSLEEYIENAWISIGAPDILIHSYFLIPRSFKMEDLVSLEENSNMIRLSAMLLRLANDLGSYKRENEMGDNPKIIQCYMNDRGASEVEAREYVKSLLCTTWKKMNKEAHTSSFSQSFIDTALNIGRMALFMYLHGDGHIIQDPEIQNRIMSLIFQPFPIQCCVE