Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006890.t1 | XP_003607873.1 | 99.6 | 677 | 3 | 0 | 1 | 677 | 1 | 677 | 0.00E+00 | 1305.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006890.t1 | Q9SY60 | 64.0 | 683 | 221 | 8 | 1 | 676 | 1 | 665 | 1.1e-231 | 804.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006890.t1 | A2Q297 | 99.6 | 677 | 3 | 0 | 1 | 677 | 1 | 677 | 0.0e+00 | 1305.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene84797 | MS.gene006890 | PPI |
| MS.gene029389 | MS.gene006890 | PPI |
| MS.gene021190 | MS.gene006890 | PPI |
| MS.gene71846 | MS.gene006890 | PPI |
| MS.gene76287 | MS.gene006890 | PPI |
| MS.gene006890 | MS.gene93470 | PPI |
| MS.gene065117 | MS.gene006890 | PPI |
| MS.gene27144 | MS.gene006890 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006890.t1 | MTR_4g083940 | 99.557 | 677 | 3 | 0 | 1 | 677 | 1 | 677 | 0.0 | 1379 |
| MS.gene006890.t1 | MTR_4g005930 | 34.697 | 660 | 407 | 10 | 28 | 676 | 49 | 695 | 5.88e-110 | 350 |
| MS.gene006890.t1 | MTR_6g045363 | 34.114 | 683 | 409 | 16 | 15 | 677 | 44 | 705 | 3.22e-104 | 334 |
| MS.gene006890.t1 | MTR_1g111120 | 33.916 | 687 | 407 | 15 | 15 | 677 | 40 | 703 | 7.47e-100 | 323 |
| MS.gene006890.t1 | MTR_1g069620 | 27.623 | 648 | 432 | 14 | 27 | 665 | 60 | 679 | 1.03e-52 | 195 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006890.t1 | AT1G10180 | 63.982 | 683 | 221 | 8 | 1 | 676 | 1 | 665 | 0.0 | 849 |
| MS.gene006890.t1 | AT1G10385 | 33.979 | 671 | 415 | 11 | 18 | 677 | 39 | 692 | 1.85e-108 | 345 |
| MS.gene006890.t1 | AT5G49830 | 35.171 | 671 | 402 | 11 | 22 | 677 | 34 | 686 | 6.05e-108 | 344 |
| MS.gene006890.t1 | AT5G49830 | 35.213 | 656 | 395 | 10 | 34 | 677 | 80 | 717 | 3.18e-106 | 340 |
| MS.gene006890.t1 | AT5G49830 | 35.213 | 656 | 395 | 10 | 34 | 677 | 111 | 748 | 1.05e-105 | 340 |
Find 127 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGCCTTCACTTCCATTTC+AGG | 0.212758 | 4.4:+29191042 | MS.gene006890:CDS |
| TCTGAAGTACTGCTCAATTT+TGG | 0.215791 | 4.4:+29189724 | MS.gene006890:CDS |
| AAATGCTAGTGTAGAATTAA+AGG | 0.251429 | 4.4:+29190859 | MS.gene006890:CDS |
| GAGGATCTTGACTGGGATTC+TGG | 0.261393 | 4.4:+29191016 | MS.gene006890:CDS |
| ATCTCCATGAGCCCACTATT+TGG | 0.273163 | 4.4:-29190821 | None:intergenic |
| GATTCTTGATATGCACTTTA+CGG | 0.276409 | 4.4:+29194050 | MS.gene006890:CDS |
| TCGGAACAACTTGCAATATT+AGG | 0.290817 | 4.4:+29190719 | MS.gene006890:CDS |
| CAATATGCACTCAAAGTTCT+TGG | 0.302770 | 4.4:+29187538 | MS.gene006890:CDS |
| TATCTGACGAGCAAGAATTC+TGG | 0.316234 | 4.4:+29191652 | MS.gene006890:CDS |
| GTACAAACTACAGGAGATAT+TGG | 0.318472 | 4.4:+29190526 | MS.gene006890:intron |
| AGAAAACATTGATGTACTTC+TGG | 0.318742 | 4.4:+29189157 | MS.gene006890:CDS |
| ATCATCTTAGACAATGTAAA+AGG | 0.322480 | 4.4:-29189512 | None:intergenic |
| AAGCATTATTCCCCAATCTA+AGG | 0.326731 | 4.4:+29186775 | MS.gene006890:CDS |
| GTTGAAATCGCACGATTTGC+GGG | 0.327235 | 4.4:+29194072 | MS.gene006890:CDS |
| CATGTTGATAATACCGCATT+TGG | 0.334966 | 4.4:-29190773 | None:intergenic |
| TGTAATTTCAATGACAATAA+AGG | 0.337606 | 4.4:+29189541 | MS.gene006890:CDS |
| TCAATGACAATAAAGGAATC+TGG | 0.346816 | 4.4:+29189548 | MS.gene006890:CDS |
| TTCTGCTAGCATCTGCATTC+AGG | 0.349261 | 4.4:+29189697 | MS.gene006890:CDS |
| GATTCCACTTATCTAATTCA+TGG | 0.350347 | 4.4:-29189046 | None:intergenic |
| AGGAATAGCGTTTACCATAC+TGG | 0.359988 | 4.4:+29190739 | MS.gene006890:CDS |
| AATGTAAAAGGAAATGTTTC+AGG | 0.360157 | 4.4:-29189500 | None:intergenic |
| GCTCTCAACAAGCATGCTAT+TGG | 0.360734 | 4.4:-29189927 | None:intergenic |
| CAAGTTTGAAGAAGCATTAG+AGG | 0.362873 | 4.4:+29189187 | MS.gene006890:CDS |
| CTGTGAACTCTTAGATCTAA+AGG | 0.366187 | 4.4:+29187493 | MS.gene006890:CDS |
| TGGTAACAGTTCATCCAGTA+TGG | 0.380863 | 4.4:-29190753 | None:intergenic |
| TCTGCAGAGTTGAAAGTCTC+AGG | 0.392765 | 4.4:+29189233 | MS.gene006890:CDS |
| AGTAAATATCTGCATTCAAT+CGG | 0.393201 | 4.4:-29190979 | None:intergenic |
| GAGAGAATCAACCTTAGATT+GGG | 0.401738 | 4.4:-29186786 | None:intergenic |
| GAGCTTAAGAAGGCCTTAGA+TGG | 0.411077 | 4.4:+29189368 | MS.gene006890:CDS |
| GGTTGAAATCGCACGATTTG+CGG | 0.414431 | 4.4:+29194071 | MS.gene006890:CDS |
| ATCTGACGAGCAAGAATTCT+GGG | 0.422635 | 4.4:+29191653 | MS.gene006890:CDS |
| ACAACTCTGTTGGTATAAAC+AGG | 0.424568 | 4.4:-29189590 | None:intergenic |
| ATAAGAATGATGACCTGAAA+TGG | 0.425331 | 4.4:-29191055 | None:intergenic |
| AGAGAGAATCAACCTTAGAT+TGG | 0.425927 | 4.4:-29186787 | None:intergenic |
| CACTTTGGAGGAAATGTACT+AGG | 0.430108 | 4.4:+29190575 | MS.gene006890:CDS |
| CTTGTAAAGGATTTAATGAC+TGG | 0.430610 | 4.4:+29189017 | MS.gene006890:CDS |
| GTCTGCTCTCATAGAAAGAA+AGG | 0.459264 | 4.4:+29189289 | MS.gene006890:CDS |
| GAAAGTAAGGAACCAAATAG+TGG | 0.459390 | 4.4:+29190809 | MS.gene006890:CDS |
| ACTGCCACTTGGTTTGCATC+AGG | 0.461751 | 4.4:+29191704 | MS.gene006890:CDS |
| GATGCTGCAAAATGAAAGTA+AGG | 0.463937 | 4.4:+29190796 | MS.gene006890:CDS |
| CAGCATTAGTTGATGTGCTA+CGG | 0.466889 | 4.4:-29189414 | None:intergenic |
| GCAAGAATTCTGGGGTGTCT+TGG | 0.467617 | 4.4:+29191662 | MS.gene006890:CDS |
| AGTGGGCTCATGGAGATTGT+AGG | 0.468495 | 4.4:+29190827 | MS.gene006890:CDS |
| AGTTCTTGACATGGCAGAAT+CGG | 0.473021 | 4.4:+29189844 | MS.gene006890:CDS |
| AGCTCAAGATATTCTTGTAA+AGG | 0.474934 | 4.4:+29189004 | MS.gene006890:CDS |
| CTATCAGTCTCAAACTGAAA+AGG | 0.482629 | 4.4:+29186808 | MS.gene006890:CDS |
| TCAGGACAGAAAGAACTTGA+TGG | 0.485886 | 4.4:-29189482 | None:intergenic |
| GGCACAGCCTCTTTAAGCTC+TGG | 0.487213 | 4.4:-29190677 | None:intergenic |
| GGGAATAACTCTTCAGATGA+AGG | 0.492767 | 4.4:+29189254 | MS.gene006890:CDS |
| AGCATTAGTTGATGTGCTAC+GGG | 0.495022 | 4.4:-29189413 | None:intergenic |
| AAGTTGTTCCGAGTCTGTCT+CGG | 0.501878 | 4.4:-29190708 | None:intergenic |
| GGAATAATGCTTTCGATGGA+AGG | 0.504051 | 4.4:-29186764 | None:intergenic |
| TCAGTTCCTCTACTGCCACT+TGG | 0.507196 | 4.4:+29191693 | MS.gene006890:CDS |
| TGCTTCTTCAAACTTGTGCT+CGG | 0.508758 | 4.4:-29189180 | None:intergenic |
| GTGGCTGGAGATGTGTTACT+TGG | 0.511571 | 4.4:+29191570 | MS.gene006890:CDS |
| GTAAATATCTGCATTCAATC+GGG | 0.512134 | 4.4:-29190978 | None:intergenic |
| TTGTCATCATCGGAAGGGCC+TGG | 0.512178 | 4.4:-29190650 | None:intergenic |
| AGTTGTTCAGTGGGCTGAGT+GGG | 0.512900 | 4.4:+29189607 | MS.gene006890:CDS |
| GGAACCAAATAGTGGGCTCA+TGG | 0.517547 | 4.4:+29190817 | MS.gene006890:CDS |
| GGCAGTGCTTGAAGACCAGC+TGG | 0.519514 | 4.4:+29189310 | MS.gene006890:CDS |
| TCTAAGGCCTTCTTAAGCTC+AGG | 0.525157 | 4.4:-29189365 | None:intergenic |
| TGAAATGGAAGTGAAGGCAG+TGG | 0.528128 | 4.4:-29191040 | None:intergenic |
| CAACAATTAGCAATTGTGGC+TGG | 0.535383 | 4.4:+29191555 | MS.gene006890:CDS |
| GTGGGCTCATGGAGATTGTA+GGG | 0.536664 | 4.4:+29190828 | MS.gene006890:CDS |
| TTGGGGAATAATGCTTTCGA+TGG | 0.537651 | 4.4:-29186768 | None:intergenic |
| CAAAGTTCTTGGCTTTCTTG+AGG | 0.537754 | 4.4:+29187549 | MS.gene006890:CDS |
| AGTTTCATCTATTCAAGAGA+GGG | 0.540337 | 4.4:+29190950 | MS.gene006890:CDS |
| CCAAAGTGCAAAACAGCCAT+TGG | 0.541109 | 4.4:-29190560 | None:intergenic |
| AGAATTCTGGGGTGTCTTGG+AGG | 0.545615 | 4.4:+29191665 | MS.gene006890:CDS |
| TCTAAAACTTCTTCAACAGA+AGG | 0.546578 | 4.4:-29189794 | None:intergenic |
| ATAACAAAGAGGATCTTGAC+TGG | 0.548772 | 4.4:+29191008 | MS.gene006890:CDS |
| CTTAGATCTAAAGGATTCTG+TGG | 0.551408 | 4.4:+29187502 | MS.gene006890:CDS |
| GTTCTTATCAAAGCACTGCC+AGG | 0.551848 | 4.4:+29190632 | MS.gene006890:CDS |
| CAATCTTCCAGAGCTTAAAG+AGG | 0.552435 | 4.4:+29190670 | MS.gene006890:CDS |
| CTCTGGAAGATTGTCATCAT+CGG | 0.552997 | 4.4:-29190660 | None:intergenic |
| GCTGCAACAATTAGCAATTG+TGG | 0.553957 | 4.4:+29191551 | MS.gene006890:CDS |
| TTAGATGGTTTGATAAAACT+CGG | 0.561757 | 4.4:+29189383 | MS.gene006890:CDS |
| GAGTCTGTCTCGGCTCTGAA+AGG | 0.562375 | 4.4:-29190698 | None:intergenic |
| ACAACTATTTGATAAATACA+TGG | 0.563452 | 4.4:+29190607 | MS.gene006890:CDS |
| GCAGACACTGTCTCAGATGA+TGG | 0.565567 | 4.4:-29189668 | None:intergenic |
| CTAGTGTAGAATTAAAGGAA+TGG | 0.569164 | 4.4:+29190864 | MS.gene006890:CDS |
| AAAGTAAGGAACCAAATAGT+GGG | 0.574422 | 4.4:+29190810 | MS.gene006890:CDS |
| GCAGATGCTAGCAGAACGTA+AGG | 0.576431 | 4.4:-29189690 | None:intergenic |
| GATTCTGTGGAGAACTTATG+TGG | 0.581709 | 4.4:+29187515 | MS.gene006890:CDS |
| TGCAAACCAAGTGGCAGTAG+AGG | 0.581969 | 4.4:-29191699 | None:intergenic |
| GTGTAGAATTAAAGGAATGG+AGG | 0.582717 | 4.4:+29190867 | MS.gene006890:CDS |
| GCTCGGCAATACCAACCAGC+TGG | 0.584269 | 4.4:-29189325 | None:intergenic |
| TCTGCCATGAATTAGATAAG+TGG | 0.584707 | 4.4:+29189042 | MS.gene006890:CDS |
| TGGTTTGATAAAACTCGGGA+AGG | 0.587347 | 4.4:+29189388 | MS.gene006890:CDS |
| GGAAGATTGTCATCATCGGA+AGG | 0.589617 | 4.4:-29190656 | None:intergenic |
| AGCTTCGAGATCACTTCTGT+CGG | 0.591992 | 4.4:+29190915 | MS.gene006890:CDS |
| TGCAATTGCAGAAAGTGAAG+AGG | 0.592066 | 4.4:-29189897 | None:intergenic |
| AGCTCAGGAAAACTAACAGA+AGG | 0.592380 | 4.4:-29189350 | None:intergenic |
| TAGATGGTTTGATAAAACTC+GGG | 0.593142 | 4.4:+29189384 | MS.gene006890:CDS |
| AAAACTAACAGAAGGTTGCT+CGG | 0.595073 | 4.4:-29189342 | None:intergenic |
| CAAATGCGGTATTATCAACA+TGG | 0.597044 | 4.4:+29190774 | MS.gene006890:CDS |
| CTGCAGAGTTGAAAGTCTCA+GGG | 0.600112 | 4.4:+29189234 | MS.gene006890:CDS |
| TAGGATATCTGAAGAAGCTG+TGG | 0.601233 | 4.4:+29188941 | MS.gene006890:intron |
| GAGTTTCATCTATTCAAGAG+AGG | 0.601341 | 4.4:+29190949 | MS.gene006890:CDS |
| TACCAACAGAGTTGTTCAGT+GGG | 0.602592 | 4.4:+29189598 | MS.gene006890:CDS |
| TTACTTGAGTGATAACAAAG+AGG | 0.604703 | 4.4:+29190997 | MS.gene006890:CDS |
| ATCTGATGAACATGTCGAGA+TGG | 0.607293 | 4.4:-29194099 | None:intergenic |
| AGCCCACTGAACAACTCTGT+TGG | 0.609878 | 4.4:-29189600 | None:intergenic |
| GAAATGGAAGTGAAGGCAGT+GGG | 0.613052 | 4.4:-29191039 | None:intergenic |
| ATGACCTGAAATGGAAGTGA+AGG | 0.617824 | 4.4:-29191046 | None:intergenic |
| GGATGAACTGTTACCAAATG+CGG | 0.618227 | 4.4:+29190760 | MS.gene006890:CDS |
| GATATCTGAAGAAGCTGTGG+AGG | 0.624804 | 4.4:+29188944 | MS.gene006890:CDS |
| TTTCAGAGCCGAGACAGACT+CGG | 0.625276 | 4.4:+29190700 | MS.gene006890:CDS |
| GTGCTTGAAGACCAGCTGGT+TGG | 0.631740 | 4.4:+29189314 | MS.gene006890:CDS |
| ATTGGAACAGCTTACACCAA+TGG | 0.638850 | 4.4:+29190544 | MS.gene006890:CDS |
| TAACAGAGACTGTTGTCATG+TGG | 0.643087 | 4.4:+29191628 | MS.gene006890:CDS |
| GAAGATTGTCATCATCGGAA+GGG | 0.643235 | 4.4:-29190655 | None:intergenic |
| AATAGCATGCTTGTTGAGAG+CGG | 0.645600 | 4.4:+29189929 | MS.gene006890:CDS |
| ATACCAACAGAGTTGTTCAG+TGG | 0.648569 | 4.4:+29189597 | MS.gene006890:CDS |
| TGGCTGGAGATGTGTTACTT+GGG | 0.652414 | 4.4:+29191571 | MS.gene006890:CDS |
| GAGGCAAACTGTGGTGACAA+TGG | 0.653080 | 4.4:-29189878 | None:intergenic |
| TAACAAAGAGGATCTTGACT+GGG | 0.655528 | 4.4:+29191009 | MS.gene006890:CDS |
| GAGTTGTTCAGTGGGCTGAG+TGG | 0.658596 | 4.4:+29189606 | MS.gene006890:CDS |
| AGAGAATCAACCTTAGATTG+GGG | 0.665099 | 4.4:-29186785 | None:intergenic |
| GGTTTGATAAAACTCGGGAA+GGG | 0.673563 | 4.4:+29189389 | MS.gene006890:CDS |
| TAGAAGAGTAGTTCTTGACA+TGG | 0.677134 | 4.4:+29189835 | MS.gene006890:CDS |
| AGTACCTGATGCAAACCAAG+TGG | 0.692309 | 4.4:-29191708 | None:intergenic |
| GAGCAAGATGGAAAGTAGCG+AGG | 0.693328 | 4.4:+29186727 | None:intergenic |
| TCTGACGAGCAAGAATTCTG+GGG | 0.728977 | 4.4:+29191654 | MS.gene006890:CDS |
| AATGGCACGAGCAATAATAG+CGG | 0.743151 | 4.4:-29194127 | None:intergenic |
| GAAAGTGAAGAGGCAAACTG+TGG | 0.758643 | 4.4:-29189887 | None:intergenic |
| TATGAGAGCAGACTTATACG+AGG | 0.759611 | 4.4:-29189279 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29186734 | 29194141 | 29186734 | ID=MS.gene006890 |
| chr4.4 | mRNA | 29186734 | 29194141 | 29186734 | ID=MS.gene006890.t1;Parent=MS.gene006890 |
| chr4.4 | exon | 29186734 | 29186829 | 29186734 | ID=MS.gene006890.t1.exon1;Parent=MS.gene006890.t1 |
| chr4.4 | CDS | 29186734 | 29186829 | 29186734 | ID=cds.MS.gene006890.t1;Parent=MS.gene006890.t1 |
| chr4.4 | exon | 29187476 | 29187570 | 29187476 | ID=MS.gene006890.t1.exon2;Parent=MS.gene006890.t1 |
| chr4.4 | CDS | 29187476 | 29187570 | 29187476 | ID=cds.MS.gene006890.t1;Parent=MS.gene006890.t1 |
| chr4.4 | exon | 29188944 | 29189976 | 29188944 | ID=MS.gene006890.t1.exon3;Parent=MS.gene006890.t1 |
| chr4.4 | CDS | 29188944 | 29189976 | 29188944 | ID=cds.MS.gene006890.t1;Parent=MS.gene006890.t1 |
| chr4.4 | exon | 29190539 | 29191063 | 29190539 | ID=MS.gene006890.t1.exon4;Parent=MS.gene006890.t1 |
| chr4.4 | CDS | 29190539 | 29191063 | 29190539 | ID=cds.MS.gene006890.t1;Parent=MS.gene006890.t1 |
| chr4.4 | exon | 29191537 | 29191725 | 29191537 | ID=MS.gene006890.t1.exon5;Parent=MS.gene006890.t1 |
| chr4.4 | CDS | 29191537 | 29191725 | 29191537 | ID=cds.MS.gene006890.t1;Parent=MS.gene006890.t1 |
| chr4.4 | exon | 29194048 | 29194141 | 29194048 | ID=MS.gene006890.t1.exon6;Parent=MS.gene006890.t1 |
| chr4.4 | CDS | 29194048 | 29194141 | 29194048 | ID=cds.MS.gene006890.t1;Parent=MS.gene006890.t1 |
| Gene Sequence |
| Protein sequence |
