AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008904

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008904.t1 MTR_8g068890 98.101 316 5 1 1 316 1 315 0.0 631
MS.gene008904.t1 MTR_5g032420 81.366 322 54 4 1 316 1 322 7.70e-179 497
MS.gene008904.t1 MTR_3g100570 74.132 317 77 3 1 316 1 313 3.85e-171 477
MS.gene008904.t1 MTR_8g086070 38.390 323 149 6 3 316 18 299 1.54e-66 211
MS.gene008904.t1 MTR_7g066690 37.299 311 166 6 6 314 17 300 1.23e-61 198
MS.gene008904.t1 MTR_4g018750 36.422 313 170 6 6 316 15 300 5.41e-61 197
MS.gene008904.t1 MTR_1g090330 33.333 303 176 4 11 312 19 296 9.28e-54 178
MS.gene008904.t1 MTR_3g462140 33.227 313 181 5 4 314 13 299 3.30e-50 169
MS.gene008904.t1 MTR_4g018750 34.783 276 151 6 6 279 15 263 1.28e-44 154
MS.gene008904.t1 MTR_4g018750 28.324 173 119 3 144 311 31 203 1.41e-15 75.9
MS.gene008904.t1 MTR_8g086070 38.991 218 94 4 3 214 18 202 3.25e-43 148
MS.gene008904.t1 MTR_1g088995 30.945 307 179 5 6 312 33 306 5.10e-40 142
MS.gene008904.t1 MTR_1g088995 29.596 223 125 4 6 228 33 223 7.00e-20 87.0
MS.gene008904.t1 MTR_4g057850 25.723 311 189 9 7 309 23 299 1.50e-19 87.8
MS.gene008904.t1 MTR_4g057850 28.426 197 120 4 115 303 9 192 1.04e-12 68.2
MS.gene008904.t1 MTR_1g097890 24.679 312 177 7 4 313 49 304 2.11e-16 78.6
MS.gene008904.t1 MTR_1g087840 26.263 297 186 9 22 313 35 303 3.96e-16 77.8
MS.gene008904.t1 MTR_4g012850 21.975 314 202 6 6 313 16 292 5.31e-16 77.4
MS.gene008904.t1 MTR_4g012850 28.436 211 116 6 9 217 117 294 6.45e-16 77.4
MS.gene008904.t1 MTR_8g027975 24.484 339 193 12 4 313 49 353 1.32e-15 77.0
MS.gene008904.t1 MTR_5g009500 21.557 334 207 8 4 313 36 338 9.32e-15 74.3
MS.gene008904.t1 MTR_2g102010 27.517 298 172 12 4 294 15 275 8.54e-14 70.9
MS.gene008904.t1 MTR_2g102010 25.397 189 113 4 22 208 126 288 5.85e-11 62.8
MS.gene008904.t1 MTR_5g073040 25.159 314 191 7 11 316 40 317 9.10e-14 71.2
MS.gene008904.t1 MTR_5g055410 27.063 303 176 12 22 313 37 305 1.34e-13 70.9
MS.gene008904.t1 MTR_4g119770 23.529 340 204 13 5 316 17 328 1.70e-13 70.5
MS.gene008904.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 2.01e-13 69.3
MS.gene008904.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 2.92e-13 68.9
MS.gene008904.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 3.99e-13 69.3
MS.gene008904.t1 MTR_4g088545 23.006 326 204 9 2 313 8 300 1.05e-11 65.1
MS.gene008904.t1 MTR_5g030530 25.723 311 197 8 9 313 14 296 5.10e-13 68.9
MS.gene008904.t1 MTR_3g104500 23.016 252 171 7 74 314 83 322 5.16e-13 68.9
MS.gene008904.t1 MTR_6g091800 26.415 212 132 7 115 311 5 207 9.34e-13 68.2
MS.gene008904.t1 MTR_7g084400 25.000 328 171 10 6 313 213 485 1.24e-12 68.6
MS.gene008904.t1 MTR_7g109135 25.714 315 191 10 9 313 25 306 1.72e-12 67.4
MS.gene008904.t1 MTR_8g092080 22.436 312 188 5 4 315 50 307 2.26e-12 67.0
MS.gene008904.t1 MTR_8g066975 26.498 317 187 12 9 313 14 296 2.50e-12 66.6
MS.gene008904.t1 MTR_4g114320 24.082 245 169 6 76 315 164 396 3.20e-12 67.0
MS.gene008904.t1 MTR_7g068340 30.337 178 105 6 144 315 103 267 5.65e-12 66.2
MS.gene008904.t1 MTR_8g076760 22.801 307 195 9 21 314 29 306 6.38e-12 65.9
MS.gene008904.t1 MTR_7g117030 24.924 329 208 11 12 315 23 337 4.34e-11 63.5
MS.gene008904.t1 MTR_7g117030 25.346 217 126 6 134 316 23 237 7.07e-11 62.8
MS.gene008904.t1 MTR_1g012030 23.457 243 170 6 76 313 179 410 6.22e-11 63.2
MS.gene008904.t1 MTR_3g025800 23.009 339 199 12 6 315 28 333 9.99e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008904.t1 AT4G24570 74.528 318 74 2 1 316 1 313 3.61e-170 474
MS.gene008904.t1 AT2G22500 75.000 316 74 1 1 316 1 311 2.35e-165 462
MS.gene008904.t1 AT5G09470 59.882 339 107 6 1 315 1 334 1.27e-124 360
MS.gene008904.t1 AT5G19760 39.498 319 147 5 3 314 15 294 1.44e-69 218
MS.gene008904.t1 AT5G58970 38.535 314 160 6 6 313 12 298 8.72e-65 206
MS.gene008904.t1 AT3G54110 37.898 314 168 6 4 316 13 300 8.83e-64 204
MS.gene008904.t1 AT1G14140 33.987 306 171 4 11 312 21 299 5.31e-52 174
MS.gene008904.t1 AT1G14140 26.601 203 123 2 9 211 121 297 3.63e-13 69.3
MS.gene008904.t1 AT4G03115 32.797 311 171 5 3 312 35 308 2.08e-46 159
MS.gene008904.t1 AT4G03115 32.476 311 172 5 3 312 67 340 3.98e-46 159
MS.gene008904.t1 AT5G58970 37.751 249 129 5 6 248 12 240 1.11e-43 151
MS.gene008904.t1 AT5G58970 26.590 173 122 3 144 311 32 204 1.70e-18 84.0
MS.gene008904.t1 AT4G03115 34.137 249 151 4 65 312 9 245 5.48e-42 146
MS.gene008904.t1 AT4G03115 34.583 240 144 4 74 312 10 237 1.16e-40 142
MS.gene008904.t1 AT4G03115 30.396 227 121 4 3 228 35 225 2.85e-24 99.0
MS.gene008904.t1 AT4G03115 30.396 227 121 4 3 228 35 225 3.58e-24 98.6
MS.gene008904.t1 AT5G01340 26.433 314 194 8 7 314 18 300 2.20e-22 95.5
MS.gene008904.t1 AT5G01340 28.272 191 114 6 122 303 11 187 3.52e-11 63.5
MS.gene008904.t1 AT1G25380 26.821 302 183 8 22 315 37 308 1.08e-16 80.1
MS.gene008904.t1 AT1G25380 26.821 302 183 8 22 315 37 308 1.08e-16 80.1
MS.gene008904.t1 AT1G34065 24.522 314 179 7 4 315 73 330 3.82e-16 78.2
MS.gene008904.t1 AT1G34065 24.522 314 179 7 4 315 80 337 4.16e-16 78.2
MS.gene008904.t1 AT3G53940 23.988 321 204 8 3 313 70 360 5.13e-16 78.2
MS.gene008904.t1 AT2G33820 24.848 330 191 11 5 313 13 306 9.59e-16 77.0
MS.gene008904.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.42e-15 76.3
MS.gene008904.t1 AT5G46800 22.535 213 145 3 110 313 1 202 7.46e-11 62.4
MS.gene008904.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.42e-15 76.3
MS.gene008904.t1 AT5G46800 22.535 213 145 3 110 313 1 202 7.46e-11 62.4
MS.gene008904.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.42e-15 76.3
MS.gene008904.t1 AT5G46800 22.535 213 145 3 110 313 1 202 7.46e-11 62.4
MS.gene008904.t1 AT5G56450 25.100 251 165 7 75 314 88 326 1.87e-15 76.3
MS.gene008904.t1 AT4G01100 25.074 339 205 13 4 313 40 358 6.15e-15 75.1
MS.gene008904.t1 AT4G01100 25.000 340 190 12 4 313 40 344 6.33e-15 75.1
MS.gene008904.t1 AT4G01100 26.792 265 157 9 78 313 38 294 1.00e-13 70.9
MS.gene008904.t1 AT2G37890 22.430 321 207 9 4 313 43 332 2.56e-13 70.1
MS.gene008904.t1 AT4G39460 23.871 310 182 5 4 313 56 311 3.20e-13 69.7
MS.gene008904.t1 AT4G39460 23.871 310 182 5 4 313 56 311 3.20e-13 69.7
MS.gene008904.t1 AT4G39460 23.871 310 182 5 4 313 56 311 3.20e-13 69.7
MS.gene008904.t1 AT2G33820 24.260 338 191 12 5 313 13 314 3.85e-13 69.3
MS.gene008904.t1 AT2G47490 27.213 305 172 12 22 313 33 300 1.03e-12 68.2
MS.gene008904.t1 AT5G66380 28.718 195 121 8 130 316 12 196 1.07e-12 68.2
MS.gene008904.t1 AT3G53940 26.425 193 125 4 132 312 73 260 1.08e-12 67.8
MS.gene008904.t1 AT4G26180 24.885 217 141 8 109 311 1 209 2.38e-12 66.6
MS.gene008904.t1 AT2G35800 24.841 314 185 10 3 314 542 806 3.12e-12 67.8
MS.gene008904.t1 AT3G55640 24.873 197 129 5 132 315 38 228 3.22e-12 65.9
MS.gene008904.t1 AT3G55640 24.873 197 129 5 132 315 38 228 3.22e-12 65.9
MS.gene008904.t1 AT1G07030 25.767 326 185 10 6 316 36 319 3.24e-12 66.6
MS.gene008904.t1 AT4G27940 24.340 341 217 10 12 314 71 408 3.69e-12 67.0
MS.gene008904.t1 AT4G26180 24.885 217 141 8 109 311 1 209 4.37e-12 66.2
MS.gene008904.t1 AT3G55640 24.623 199 135 4 126 315 36 228 6.26e-12 65.9
MS.gene008904.t1 AT3G55640 20.312 320 217 6 6 316 38 328 6.48e-11 62.8
MS.gene008904.t1 AT3G55640 24.623 199 135 4 126 315 36 228 6.26e-12 65.9
MS.gene008904.t1 AT3G55640 20.312 320 217 6 6 316 38 328 6.48e-11 62.8
MS.gene008904.t1 AT3G51870 30.337 178 111 6 141 315 104 271 6.57e-12 66.2
MS.gene008904.t1 AT3G51870 23.986 296 183 10 20 313 105 360 4.05e-11 63.9
MS.gene008904.t1 AT2G33820 23.596 267 146 9 5 243 13 249 1.18e-11 64.7
MS.gene008904.t1 AT2G33820 25.269 186 130 5 130 310 20 201 9.12e-11 62.0
MS.gene008904.t1 AT5G17400 23.793 290 185 7 6 287 14 275 3.00e-11 63.5
MS.gene008904.t1 AT5G01500 28.492 179 115 7 140 315 131 299 8.55e-11 62.8

Find 125 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATCCTATGATGAGTTTA+AGG 0.221358 8.2:-34039155 MS.gene008904:CDS
CGGCCCAACACGGGGCGGTT+GGG 0.300952 8.2:+34039582 None:intergenic
GGGCTGCTCGCCGGTGGGAT+TGG 0.301666 8.2:-34039376 MS.gene008904:CDS
TGGCGCGGCGGTCGGGAATC+CGG 0.321453 8.2:-34039356 MS.gene008904:CDS
GTGGGATTGGCGCGGCGGTC+GGG 0.359376 8.2:-34039363 MS.gene008904:CDS
CGGATTTATAGTTTCTTCTC+TGG 0.361423 8.2:+34039284 None:intergenic
TGCTAATGCTGGTCGGATAT+TGG 0.367208 8.2:+34039645 None:intergenic
CGGCCCAACCGCCCCGTGTT+GGG 0.379107 8.2:-34039585 MS.gene008904:CDS
ACAGGTTCGTAAGTTGCTTA+AGG 0.385790 8.2:-34038846 MS.gene008904:CDS
GGTGGGATTGGCGCGGCGGT+CGG 0.398701 8.2:-34039364 MS.gene008904:CDS
TCGGCCCAACACGGGGCGGT+TGG 0.400715 8.2:+34039581 None:intergenic
GGCGGTTGGGCCGTCACGGC+CGG 0.409237 8.2:+34039595 None:intergenic
ACGGCCCAACCGCCCCGTGT+TGG 0.410440 8.2:-34039586 MS.gene008904:CDS
TTGCCGTTGACAAGCAAGAT+TGG 0.420958 8.2:-34039403 MS.gene008904:CDS
GGTTGGATGAACGATGGGCT+TGG 0.421393 8.2:-34039115 MS.gene008904:CDS
ACAAACAACACAACGGTAAA+CGG 0.423325 8.2:+34038878 None:intergenic
GTTAGTTACCGCGTCACAAC+TGG 0.425239 8.2:-34039176 MS.gene008904:CDS
GTTGTTTGTTACATTGGAAC+AGG 0.435604 8.2:-34038864 MS.gene008904:CDS
GGCATAGCTTCCATCATTGC+AGG 0.435640 8.2:-34039739 MS.gene008904:CDS
GGTCCCTGCCTTGTAATTGT+AGG 0.442748 8.2:+34038899 None:intergenic
ATTGGAGCGGGGCTGCTCGC+CGG 0.443449 8.2:-34039385 MS.gene008904:CDS
CGTTGTGTTGTTTGTTACAT+TGG 0.449852 8.2:-34038870 MS.gene008904:CDS
TCCAACCCGGTTCGGTTCAT+CGG 0.451801 8.2:-34039621 MS.gene008904:CDS
GGATTTATAGTTTCTTCTCT+GGG 0.452868 8.2:+34039285 None:intergenic
TCCCTCGTCTTTCGCCATCC+TGG 0.455818 8.2:+34039246 None:intergenic
CAATCAATGGCACCGGAGTA+AGG 0.460053 8.2:+34038977 None:intergenic
GTTGGATGAACGATGGGCTT+GGG 0.460335 8.2:-34039114 MS.gene008904:CDS
GGAGTAAGGCGGAGGCGATC+CGG 0.461442 8.2:+34038991 None:intergenic
GTCTTTCGCCATCCTGGTGA+TGG 0.464552 8.2:+34039252 None:intergenic
GGAGCGGGGCTGCTCGCCGG+TGG 0.465110 8.2:-34039382 MS.gene008904:CDS
TGGTGTTAAGCTAGTCCAAC+AGG 0.465340 8.2:-34039551 MS.gene008904:CDS
CGGTGACCGATGAACCGAAC+CGG 0.466185 8.2:+34039615 None:intergenic
AACGGCGATCGGCCCAACAC+GGG 0.470119 8.2:+34039573 None:intergenic
GGCGGCGGTTGCGTCGAATC+CGG 0.471512 8.2:-34039056 MS.gene008904:CDS
TTGACAAGCAAGATTGGAGC+GGG 0.472465 8.2:-34039397 MS.gene008904:CDS
ACGGGGCGGTTGGGCCGTCA+CGG 0.473765 8.2:+34039591 None:intergenic
ATTAGCATTCCAACCCGGTT+CGG 0.477760 8.2:-34039629 MS.gene008904:CDS
CGGTGGATGTGATTAAGACT+AGG 0.482921 8.2:-34039036 MS.gene008904:CDS
CAACGGCGATCGGCCCAACA+CGG 0.483085 8.2:+34039572 None:intergenic
TTGAAGACTGTTCGTGCTGA+GGG 0.486561 8.2:-34038950 MS.gene008904:CDS
GACCGCCGCGCCAATCCCAC+CGG 0.488627 8.2:+34039366 None:intergenic
TATTCCTACAATTACAAGGC+AGG 0.491525 8.2:-34038903 MS.gene008904:CDS
TGTTCGTGCTGAGGGTCCTA+TGG 0.492843 8.2:-34038942 MS.gene008904:CDS
TCTCTATTCCACCACTCGTA+TGG 0.493057 8.2:-34039476 MS.gene008904:CDS
TGTTGAACACCCTGCAATGA+TGG 0.493211 8.2:+34039729 None:intergenic
AAGGTTCGAATGCAGCTTCA+AGG 0.496054 8.2:-34039688 MS.gene008904:CDS
TCATCGGTCACCGGCCGTGA+CGG 0.498790 8.2:-34039605 MS.gene008904:CDS
CTCTATTCCACCACTCGTAT+GGG 0.500478 8.2:-34039475 MS.gene008904:CDS
GCTGGTCGGATATTGGTTGT+AGG 0.505194 8.2:+34039652 None:intergenic
ATGAATATGAAGGTGGAGGC+CGG 0.509995 8.2:-34039010 MS.gene008904:CDS
CGTGTTGGGCCGATCGCCGT+TGG 0.512777 8.2:-34039571 MS.gene008904:CDS
GTGTCTCTGCCACCGTTCTC+CGG 0.514808 8.2:-34039504 MS.gene008904:CDS
CCGTTGTCGACGCCATCACC+AGG 0.516545 8.2:-34039264 MS.gene008904:CDS
AACGGCAAAGTACCGGAGAT+AGG 0.516962 8.2:+34039418 None:intergenic
AGCTTAACACCAACGGCGAT+CGG 0.517276 8.2:+34039562 None:intergenic
CGGCTGATGTGGCAATGGTT+AGG 0.521248 8.2:-34039336 MS.gene008904:CDS
AGTCTTCAAAGCACAATCAA+TGG 0.523057 8.2:+34038964 None:intergenic
GAGCGGGGCTGCTCGCCGGT+GGG 0.525418 8.2:-34039381 MS.gene008904:CDS
GGTCGGGAATCCGGCTGATG+TGG 0.525455 8.2:-34039347 MS.gene008904:CDS
AATAATCGCAATGGGTGTGA+AGG 0.526131 8.2:-34039776 None:intergenic
CTAACCATTGCCACATCAGC+CGG 0.528201 8.2:+34039337 None:intergenic
AAATGGTCTGATCCTATCTC+CGG 0.538460 8.2:-34039430 MS.gene008904:CDS
ACCGATGAACCGAACCGGGT+TGG 0.539068 8.2:+34039620 None:intergenic
GCTTGTCAACGGCAAAGTAC+CGG 0.541832 8.2:+34039411 None:intergenic
TTTGAAGACTGTTCGTGCTG+AGG 0.545035 8.2:-34038951 MS.gene008904:CDS
ACGAGGGAGTTACTAGCCTA+TGG 0.549664 8.2:-34039231 MS.gene008904:CDS
TGTGGCAATGGTTAGGATGC+AGG 0.550254 8.2:-34039329 MS.gene008904:CDS
AAACCTTTGTAAAGAGCCAT+AGG 0.551305 8.2:+34038926 None:intergenic
TCAGAGATGAACCGCGCCAT+AGG 0.555710 8.2:+34039215 None:intergenic
GCTCGCCGGTGGGATTGGCG+CGG 0.558280 8.2:-34039371 MS.gene008904:CDS
GGTGACCGATGAACCGAACC+GGG 0.561504 8.2:+34039616 None:intergenic
CCTGGTGATGGCGTCGACAA+CGG 0.566152 8.2:+34039264 None:intergenic
CGCCGGTGGGATTGGCGCGG+CGG 0.566467 8.2:-34039368 MS.gene008904:CDS
GTGCTGCTACACCTTCCTGT+TGG 0.566711 8.2:+34039536 None:intergenic
GGATCGCCTCCGCCTTACTC+CGG 0.567757 8.2:-34038989 MS.gene008904:CDS
ATAATCGCAATGGGTGTGAA+GGG 0.570571 8.2:-34039775 None:intergenic
TAGTTTCTTCTCTGGGCCGA+CGG 0.570902 8.2:+34039292 None:intergenic
GAGAACGGTGGCAGAGACAC+CGG 0.571761 8.2:+34039507 None:intergenic
CCGGGTTGGAATGCTAATGC+TGG 0.573037 8.2:+34039634 None:intergenic
GGTGTGAAGGGTTTCGTCGA+AGG 0.574034 8.2:-34039763 MS.gene008904:CDS
ATGGCACCGGAGTAAGGCGG+AGG 0.575695 8.2:+34038983 None:intergenic
ATTCCTACAATTACAAGGCA+GGG 0.578803 8.2:-34038902 MS.gene008904:CDS
CGGTTCGGTTCATCGGTCAC+CGG 0.580843 8.2:-34039614 MS.gene008904:CDS
GGTGGATGTGATTAAGACTA+GGG 0.581075 8.2:-34039035 MS.gene008904:CDS
GGTCCTATGGCTCTTTACAA+AGG 0.588648 8.2:-34038929 MS.gene008904:CDS
GGTGATGAATATGAAGGTGG+AGG 0.589447 8.2:-34039014 MS.gene008904:CDS
ACGGCGATCGGCCCAACACG+GGG 0.592000 8.2:+34039574 None:intergenic
CAAAGCACAATCAATGGCAC+CGG 0.593300 8.2:+34038970 None:intergenic
GACTAGGGTGATGAATATGA+AGG 0.600049 8.2:-34039020 MS.gene008904:CDS
TTGTGACGCGGTAACTAACA+TGG 0.601663 8.2:+34039180 None:intergenic
AAAATGGTTGGATGAACGAT+GGG 0.602734 8.2:-34039120 MS.gene008904:CDS
ATAGAGTCCCATACGAGTGG+TGG 0.603119 8.2:+34039468 None:intergenic
CACCAGGATGGCGAAAGACG+AGG 0.603337 8.2:-34039248 MS.gene008904:CDS
CTAGTCTTAATCACATCCAC+CGG 0.603477 8.2:+34039037 None:intergenic
GAATCCGGCTGATGTGGCAA+TGG 0.605065 8.2:-34039341 MS.gene008904:CDS
TGGTGGAATAGAGACACTGC+CGG 0.605680 8.2:+34039485 None:intergenic
ACACCCTCTTGATCTCATCA+AGG 0.606884 8.2:-34039707 MS.gene008904:CDS
GTGAAGGGTTTCGTCGAAGG+AGG 0.607310 8.2:-34039760 MS.gene008904:CDS
AACCGCCGCCACAAAACCAG+CGG 0.608991 8.2:+34039069 None:intergenic
CGAACCTTGATGAGATCAAG+AGG 0.611493 8.2:+34039703 None:intergenic
GGCCGACGGAAGTCTTCCGT+CGG 0.612610 8.2:+34039306 None:intergenic
GGCGGTTGCGTCGAATCCGG+TGG 0.613152 8.2:-34039053 MS.gene008904:CDS
ATAGAGACACTGCCGGAGAA+CGG 0.615334 8.2:+34039492 None:intergenic
ACCAGGATGGCGAAAGACGA+GGG 0.622914 8.2:-34039247 MS.gene008904:CDS
ATGGTTAGGATGCAGGCCGA+CGG 0.622979 8.2:-34039322 MS.gene008904:CDS
TAGGGTGATGAATATGAAGG+TGG 0.625045 8.2:-34039017 MS.gene008904:CDS
GGCCGACGGAAGACTTCCGT+CGG 0.629424 8.2:-34039308 MS.gene008904:CDS
GCTCCAATCTTGCTTGTCAA+CGG 0.630329 8.2:+34039400 None:intergenic
CCAGCATTAGCATTCCAACC+CGG 0.630776 8.2:-34039634 MS.gene008904:CDS
TTGGACTAGCTTAACACCAA+CGG 0.639677 8.2:+34039555 None:intergenic
TGTCGACGCCATCACCAGGA+TGG 0.643772 8.2:-34039260 MS.gene008904:CDS
GTTAAGCTAGTCCAACAGGA+AGG 0.649653 8.2:-34039547 MS.gene008904:CDS
GCATAGCTTCCATCATTGCA+GGG 0.651032 8.2:-34039738 MS.gene008904:CDS
GTTGGAATGCTAATGCTGGT+CGG 0.653431 8.2:+34039638 None:intergenic
ATCATAGAGTCCCATACGAG+TGG 0.658486 8.2:+34039465 None:intergenic
GCGATCGGCCCAACACGGGG+CGG 0.664344 8.2:+34039577 None:intergenic
GTTGACAAGCAAGATTGGAG+CGG 0.666908 8.2:-34039398 MS.gene008904:CDS
ACGCGGTAACTAACATGGCG+CGG 0.672856 8.2:+34039185 None:intergenic
GAGACACTGCCGGAGAACGG+TGG 0.689533 8.2:+34039495 None:intergenic
ATAGGATGCCAGTTGTGACG+CGG 0.695379 8.2:+34039168 None:intergenic
CAATGTAACAAACAACACAA+CGG 0.699064 8.2:+34038871 None:intergenic
GAACCTTGATGAGATCAAGA+GGG 0.713345 8.2:+34039704 None:intergenic
TGACAAGCAAGATTGGAGCG+GGG 0.724268 8.2:-34039396 MS.gene008904:CDS
TCAATGGCACCGGAGTAAGG+CGG 0.732141 8.2:+34038980 None:intergenic
GGAGTTACTAGCCTATGGCG+CGG 0.742480 8.2:-34039226 MS.gene008904:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATATGATGAAGAAAAAA+TGG - chr8.2:34039157-34039176 MS.gene008904:CDS 20.0%
!!! AAAAGATTTTGAAAAATGGT+TGG - chr8.2:34039472-34039491 MS.gene008904:CDS 20.0%
!!! AAGGAAAAGATTTTGAAAAA+TGG - chr8.2:34039468-34039487 MS.gene008904:CDS 20.0%
!!! GTTTTATTCCTACAATTACA+AGG - chr8.2:34039697-34039716 MS.gene008904:CDS 25.0%
CAATGTAACAAACAACACAA+CGG + chr8.2:34039736-34039755 None:intergenic 30.0%
GGATTTATAGTTTCTTCTCT+GGG + chr8.2:34039322-34039341 None:intergenic 30.0%
! CTTTTCCTTAAACTCATCAT+AGG + chr8.2:34039457-34039476 None:intergenic 30.0%
AAAAATGGTTGGATGAACGA+TGG - chr8.2:34039483-34039502 MS.gene008904:CDS 35.0%
AAAATGGTTGGATGAACGAT+GGG - chr8.2:34039484-34039503 MS.gene008904:CDS 35.0%
AAACCTTTGTAAAGAGCCAT+AGG + chr8.2:34039681-34039700 None:intergenic 35.0%
ACAAACAACACAACGGTAAA+CGG + chr8.2:34039729-34039748 None:intergenic 35.0%
AGTCTTCAAAGCACAATCAA+TGG + chr8.2:34039643-34039662 None:intergenic 35.0%
ATTCCTACAATTACAAGGCA+GGG - chr8.2:34039702-34039721 MS.gene008904:CDS 35.0%
CGGATTTATAGTTTCTTCTC+TGG + chr8.2:34039323-34039342 None:intergenic 35.0%
TATTCCTACAATTACAAGGC+AGG - chr8.2:34039701-34039720 MS.gene008904:CDS 35.0%
! GTTGTTTGTTACATTGGAAC+AGG - chr8.2:34039740-34039759 MS.gene008904:CDS 35.0%
!! CGTTGTGTTGTTTGTTACAT+TGG - chr8.2:34039734-34039753 MS.gene008904:CDS 35.0%
AAATGGTCTGATCCTATCTC+CGG - chr8.2:34039174-34039193 MS.gene008904:CDS 40.0%
ACAGGTTCGTAAGTTGCTTA+AGG - chr8.2:34039758-34039777 MS.gene008904:CDS 40.0%
GAACCTTGATGAGATCAAGA+GGG + chr8.2:34038903-34038922 None:intergenic 40.0%
GACTAGGGTGATGAATATGA+AGG - chr8.2:34039584-34039603 MS.gene008904:CDS 40.0%
GGAAAAACTAGCTGTCACAT+GGG + chr8.2:34039517-34039536 None:intergenic 40.0%
GGCATCCTATGATGAGTTTA+AGG - chr8.2:34039449-34039468 MS.gene008904:CDS 40.0%
GGTGGATGTGATTAAGACTA+GGG - chr8.2:34039569-34039588 MS.gene008904:CDS 40.0%
TAGGGTGATGAATATGAAGG+TGG - chr8.2:34039587-34039606 MS.gene008904:CDS 40.0%
!! CTAGTCTTAATCACATCCAC+CGG + chr8.2:34039570-34039589 None:intergenic 40.0%
!! TTGGACTAGCTTAACACCAA+CGG + chr8.2:34039052-34039071 None:intergenic 40.0%
!!! TAGTTTTTCCGCTGGTTTTG+TGG - chr8.2:34039527-34039546 MS.gene008904:CDS 40.0%
AAGGTTCGAATGCAGCTTCA+AGG - chr8.2:34038916-34038935 MS.gene008904:CDS 45.0%
ACACCCTCTTGATCTCATCA+AGG - chr8.2:34038897-34038916 MS.gene008904:CDS 45.0%
ATCATAGAGTCCCATACGAG+TGG + chr8.2:34039142-34039161 None:intergenic 45.0%
ATGAATATGAAGGTGGAGGC+CGG - chr8.2:34039594-34039613 MS.gene008904:CDS 45.0%
ATTAGCATTCCAACCCGGTT+CGG - chr8.2:34038975-34038994 MS.gene008904:CDS 45.0%
CAAAGCACAATCAATGGCAC+CGG + chr8.2:34039637-34039656 None:intergenic 45.0%
CGAACCTTGATGAGATCAAG+AGG + chr8.2:34038904-34038923 None:intergenic 45.0%
CGGAAAAACTAGCTGTCACA+TGG + chr8.2:34039518-34039537 None:intergenic 45.0%
CGGTGGATGTGATTAAGACT+AGG - chr8.2:34039568-34039587 MS.gene008904:CDS 45.0%
CTCTATTCCACCACTCGTAT+GGG - chr8.2:34039129-34039148 MS.gene008904:CDS 45.0%
GCATAGCTTCCATCATTGCA+GGG - chr8.2:34038866-34038885 MS.gene008904:CDS 45.0%
GCTCCAATCTTGCTTGTCAA+CGG + chr8.2:34039207-34039226 None:intergenic 45.0%
GGTCCTATGGCTCTTTACAA+AGG - chr8.2:34039675-34039694 MS.gene008904:CDS 45.0%
GGTGATGAATATGAAGGTGG+AGG - chr8.2:34039590-34039609 MS.gene008904:CDS 45.0%
GTTAAGCTAGTCCAACAGGA+AGG - chr8.2:34039057-34039076 MS.gene008904:CDS 45.0%
GTTGACAAGCAAGATTGGAG+CGG - chr8.2:34039206-34039225 MS.gene008904:CDS 45.0%
GTTGGAATGCTAATGCTGGT+CGG + chr8.2:34038969-34038988 None:intergenic 45.0%
TCTCTATTCCACCACTCGTA+TGG - chr8.2:34039128-34039147 MS.gene008904:CDS 45.0%
TGCTAATGCTGGTCGGATAT+TGG + chr8.2:34038962-34038981 None:intergenic 45.0%
TGTTGAACACCCTGCAATGA+TGG + chr8.2:34038878-34038897 None:intergenic 45.0%
TTGAAGACTGTTCGTGCTGA+GGG - chr8.2:34039654-34039673 MS.gene008904:CDS 45.0%
TTGACAAGCAAGATTGGAGC+GGG - chr8.2:34039207-34039226 MS.gene008904:CDS 45.0%
TTGCCGTTGACAAGCAAGAT+TGG - chr8.2:34039201-34039220 MS.gene008904:CDS 45.0%
TTGTGACGCGGTAACTAACA+TGG + chr8.2:34039427-34039446 None:intergenic 45.0%
TTTGAAGACTGTTCGTGCTG+AGG - chr8.2:34039653-34039672 MS.gene008904:CDS 45.0%
! TGGTGTTAAGCTAGTCCAAC+AGG - chr8.2:34039053-34039072 MS.gene008904:CDS 45.0%
!!! GGTGTAGCAGCACTTTTTTC+CGG - chr8.2:34039078-34039097 MS.gene008904:CDS 45.0%
!!! TTTTTCCGCTGGTTTTGTGG+CGG - chr8.2:34039530-34039549 MS.gene008904:CDS 45.0%
AGCTTAACACCAACGGCGAT+CGG + chr8.2:34039045-34039064 None:intergenic 50.0%
ATAGAGACACTGCCGGAGAA+CGG + chr8.2:34039115-34039134 None:intergenic 50.0%
ATAGAGTCCCATACGAGTGG+TGG + chr8.2:34039139-34039158 None:intergenic 50.0%
ATAGGATGCCAGTTGTGACG+CGG + chr8.2:34039439-34039458 None:intergenic 50.0%
CCAGCATTAGCATTCCAACC+CGG - chr8.2:34038970-34038989 MS.gene008904:CDS 50.0%
CTAACCATTGCCACATCAGC+CGG + chr8.2:34039270-34039289 None:intergenic 50.0%
GCTTGTCAACGGCAAAGTAC+CGG + chr8.2:34039196-34039215 None:intergenic 50.0%
GGCATAGCTTCCATCATTGC+AGG - chr8.2:34038865-34038884 MS.gene008904:CDS 50.0%
GGTCCCTGCCTTGTAATTGT+AGG + chr8.2:34039708-34039727 None:intergenic 50.0%
GTTAGTTACCGCGTCACAAC+TGG - chr8.2:34039428-34039447 MS.gene008904:CDS 50.0%
TAGTTTCTTCTCTGGGCCGA+CGG + chr8.2:34039315-34039334 None:intergenic 50.0%
TGACAAGCAAGATTGGAGCG+GGG - chr8.2:34039208-34039227 MS.gene008904:CDS 50.0%
TGGTGGAATAGAGACACTGC+CGG + chr8.2:34039122-34039141 None:intergenic 50.0%
TGTGGCAATGGTTAGGATGC+AGG - chr8.2:34039275-34039294 MS.gene008904:CDS 50.0%
! ACGAGGGAGTTACTAGCCTA+TGG - chr8.2:34039373-34039392 MS.gene008904:CDS 50.0%
! CAATCAATGGCACCGGAGTA+AGG + chr8.2:34039630-34039649 None:intergenic 50.0%
! GCTGGTCGGATATTGGTTGT+AGG + chr8.2:34038955-34038974 None:intergenic 50.0%
! GTTGGATGAACGATGGGCTT+GGG - chr8.2:34039490-34039509 MS.gene008904:CDS 50.0%
!! AACGGCAAAGTACCGGAGAT+AGG + chr8.2:34039189-34039208 None:intergenic 50.0%
!!! GTGACAGCTAGTTTTTCCGC+TGG - chr8.2:34039519-34039538 MS.gene008904:CDS 50.0%
ACCAGGATGGCGAAAGACGA+GGG - chr8.2:34039357-34039376 MS.gene008904:CDS 55.0%
ACGCGGTAACTAACATGGCG+CGG + chr8.2:34039422-34039441 None:intergenic 55.0%
ATGGTTAGGATGCAGGCCGA+CGG - chr8.2:34039282-34039301 MS.gene008904:CDS 55.0%
CGGCTGATGTGGCAATGGTT+AGG - chr8.2:34039268-34039287 MS.gene008904:CDS 55.0%
GAATCCGGCTGATGTGGCAA+TGG - chr8.2:34039263-34039282 MS.gene008904:CDS 55.0%
GGTGTGAAGGGTTTCGTCGA+AGG - chr8.2:34038841-34038860 MS.gene008904:CDS 55.0%
GTCTTTCGCCATCCTGGTGA+TGG + chr8.2:34039355-34039374 None:intergenic 55.0%
GTGAAGGGTTTCGTCGAAGG+AGG - chr8.2:34038844-34038863 MS.gene008904:CDS 55.0%
TCAGAGATGAACCGCGCCAT+AGG + chr8.2:34039392-34039411 None:intergenic 55.0%
TGTTCGTGCTGAGGGTCCTA+TGG - chr8.2:34039662-34039681 MS.gene008904:CDS 55.0%
! CCGGGTTGGAATGCTAATGC+TGG + chr8.2:34038973-34038992 None:intergenic 55.0%
! GGAGTTACTAGCCTATGGCG+CGG - chr8.2:34039378-34039397 MS.gene008904:CDS 55.0%
! GGTTGGATGAACGATGGGCT+TGG - chr8.2:34039489-34039508 MS.gene008904:CDS 55.0%
! GTGCTGCTACACCTTCCTGT+TGG + chr8.2:34039071-34039090 None:intergenic 55.0%
! TCAATGGCACCGGAGTAAGG+CGG + chr8.2:34039627-34039646 None:intergenic 55.0%
!! TCCAACCCGGTTCGGTTCAT+CGG - chr8.2:34038983-34039002 MS.gene008904:CDS 55.0%
AACCGCCGCCACAAAACCAG+CGG + chr8.2:34039538-34039557 None:intergenic 60.0%
ACCGATGAACCGAACCGGGT+TGG + chr8.2:34038987-34039006 None:intergenic 60.0%
CACCAGGATGGCGAAAGACG+AGG - chr8.2:34039356-34039375 MS.gene008904:CDS 60.0%
CGGTGACCGATGAACCGAAC+CGG + chr8.2:34038992-34039011 None:intergenic 60.0%
GAGAACGGTGGCAGAGACAC+CGG + chr8.2:34039100-34039119 None:intergenic 60.0%
GGTGACCGATGAACCGAACC+GGG + chr8.2:34038991-34039010 None:intergenic 60.0%
GTGTCTCTGCCACCGTTCTC+CGG - chr8.2:34039100-34039119 MS.gene008904:CDS 60.0%
TCCCTCGTCTTTCGCCATCC+TGG + chr8.2:34039361-34039380 None:intergenic 60.0%
TGTCGACGCCATCACCAGGA+TGG - chr8.2:34039344-34039363 MS.gene008904:CDS 60.0%
!! CCTGGTGATGGCGTCGACAA+CGG + chr8.2:34039343-34039362 None:intergenic 60.0%
!! CGGTTCGGTTCATCGGTCAC+CGG - chr8.2:34038990-34039009 MS.gene008904:CDS 60.0%
!!! TTCCGCTGGTTTTGTGGCGG+CGG - chr8.2:34039533-34039552 MS.gene008904:CDS 60.0%
AACGGCGATCGGCCCAACAC+GGG + chr8.2:34039034-34039053 None:intergenic 65.0%
CAACGGCGATCGGCCCAACA+CGG + chr8.2:34039035-34039054 None:intergenic 65.0%
CCGTTGTCGACGCCATCACC+AGG - chr8.2:34039340-34039359 MS.gene008904:CDS 65.0%
GAGACACTGCCGGAGAACGG+TGG + chr8.2:34039112-34039131 None:intergenic 65.0%
GGAGTAAGGCGGAGGCGATC+CGG + chr8.2:34039616-34039635 None:intergenic 65.0%
GGATCGCCTCCGCCTTACTC+CGG - chr8.2:34039615-34039634 MS.gene008904:CDS 65.0%
GGCCGACGGAAGACTTCCGT+CGG - chr8.2:34039296-34039315 MS.gene008904:CDS 65.0%
GGCCGACGGAAGTCTTCCGT+CGG + chr8.2:34039301-34039320 None:intergenic 65.0%
GGTCGGGAATCCGGCTGATG+TGG - chr8.2:34039257-34039276 MS.gene008904:CDS 65.0%
TCATCGGTCACCGGCCGTGA+CGG - chr8.2:34038999-34039018 MS.gene008904:CDS 65.0%
! ATGGCACCGGAGTAAGGCGG+AGG + chr8.2:34039624-34039643 None:intergenic 65.0%
ACGGCGATCGGCCCAACACG+GGG + chr8.2:34039033-34039052 None:intergenic 70.0%
ATTGGAGCGGGGCTGCTCGC+CGG - chr8.2:34039219-34039238 MS.gene008904:CDS 70.0%
CGTGTTGGGCCGATCGCCGT+TGG - chr8.2:34039033-34039052 MS.gene008904:CDS 70.0%
GGCGGCGGTTGCGTCGAATC+CGG - chr8.2:34039548-34039567 MS.gene008904:CDS 70.0%
GGCGGTTGCGTCGAATCCGG+TGG - chr8.2:34039551-34039570 MS.gene008904:CDS 70.0%
ACGGCCCAACCGCCCCGTGT+TGG - chr8.2:34039018-34039037 MS.gene008904:CDS 75.0%
ACGGGGCGGTTGGGCCGTCA+CGG + chr8.2:34039016-34039035 None:intergenic 75.0%
CGGCCCAACACGGGGCGGTT+GGG + chr8.2:34039025-34039044 None:intergenic 75.0%
CGGCCCAACCGCCCCGTGTT+GGG - chr8.2:34039019-34039038 MS.gene008904:CDS 75.0%
GACCGCCGCGCCAATCCCAC+CGG + chr8.2:34039241-34039260 None:intergenic 75.0%
GCGATCGGCCCAACACGGGG+CGG + chr8.2:34039030-34039049 None:intergenic 75.0%
GGGCTGCTCGCCGGTGGGAT+TGG - chr8.2:34039228-34039247 MS.gene008904:CDS 75.0%
TCGGCCCAACACGGGGCGGT+TGG + chr8.2:34039026-34039045 None:intergenic 75.0%
TGGCGCGGCGGTCGGGAATC+CGG - chr8.2:34039248-34039267 MS.gene008904:CDS 75.0%
! GCTCGCCGGTGGGATTGGCG+CGG - chr8.2:34039233-34039252 MS.gene008904:CDS 75.0%
! GGTGGGATTGGCGCGGCGGT+CGG - chr8.2:34039240-34039259 MS.gene008904:CDS 75.0%
! GTGGGATTGGCGCGGCGGTC+GGG - chr8.2:34039241-34039260 MS.gene008904:CDS 75.0%
! GAGCGGGGCTGCTCGCCGGT+GGG - chr8.2:34039223-34039242 MS.gene008904:CDS 80.0%
! GGCGGTTGGGCCGTCACGGC+CGG + chr8.2:34039012-34039031 None:intergenic 80.0%
!! CGCCGGTGGGATTGGCGCGG+CGG - chr8.2:34039236-34039255 MS.gene008904:CDS 80.0%
! GGAGCGGGGCTGCTCGCCGG+TGG - chr8.2:34039222-34039241 MS.gene008904:CDS 85.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 34038838 34039788 34038838 ID=MS.gene008904
chr8.2 mRNA 34038838 34039788 34038838 ID=MS.gene008904.t1;Parent=MS.gene008904
chr8.2 exon 34038838 34039788 34038838 ID=MS.gene008904.t1.exon1;Parent=MS.gene008904.t1
chr8.2 CDS 34038838 34039788 34038838 ID=cds.MS.gene008904.t1;Parent=MS.gene008904.t1
Gene Sequence

>MS.gene008904

ATGGGTGTGAAGGGTTTCGTCGAAGGAGGCATAGCTTCCATCATTGCAGGGTGTTCAACACACCCTCTTGATCTCATCAAGGTTCGAATGCAGCTTCAAGGAGAGAATAGTACTCCTACAACCAATATCCGACCAGCATTAGCATTCCAACCCGGTTCGGTTCATCGGTCACCGGCCGTGACGGCCCAACCGCCCCGTGTTGGGCCGATCGCCGTTGGTGTTAAGCTAGTCCAACAGGAAGGTGTAGCAGCACTTTTTTCCGGTGTCTCTGCCACCGTTCTCCGGCAGTGTCTCTATTCCACCACTCGTATGGGACTCTATGATATGATGAAGAAAAAATGGTCTGATCCTATCTCCGGTACTTTGCCGTTGACAAGCAAGATTGGAGCGGGGCTGCTCGCCGGTGGGATTGGCGCGGCGGTCGGGAATCCGGCTGATGTGGCAATGGTTAGGATGCAGGCCGACGGAAGACTTCCGTCGGCCCAGAGAAGAAACTATAAATCCGTTGTCGACGCCATCACCAGGATGGCGAAAGACGAGGGAGTTACTAGCCTATGGCGCGGTTCATCTCTGACAGTGAACCGCGCCATGTTAGTTACCGCGTCACAACTGGCATCCTATGATGAGTTTAAGGAAAAGATTTTGAAAAATGGTTGGATGAACGATGGGCTTGGGACCCATGTGACAGCTAGTTTTTCCGCTGGTTTTGTGGCGGCGGTTGCGTCGAATCCGGTGGATGTGATTAAGACTAGGGTGATGAATATGAAGGTGGAGGCCGGATCGCCTCCGCCTTACTCCGGTGCCATTGATTGTGCTTTGAAGACTGTTCGTGCTGAGGGTCCTATGGCTCTTTACAAAGGTTTTATTCCTACAATTACAAGGCAGGGACCGTTTACCGTTGTGTTGTTTGTTACATTGGAACAGGTTCGTAAGTTGCTTAAGGATTTCTAA
Protein sequence

>MS.gene008904.t1

MGVKGFVEGGIASIIAGCSTHPLDLIKVRMQLQGENSTPTTNIRPALAFQPGSVHRSPAVTAQPPRVGPIAVGVKLVQQEGVAALFSGVSATVLRQCLYSTTRMGLYDMMKKKWSDPISGTLPLTSKIGAGLLAGGIGAAVGNPADVAMVRMQADGRLPSAQRRNYKSVVDAITRMAKDEGVTSLWRGSSLTVNRAMLVTASQLASYDEFKEKILKNGWMNDGLGTHVTASFSAGFVAAVASNPVDVIKTRVMNMKVEAGSPPPYSGAIDCALKTVRAEGPMALYKGFIPTITRQGPFTVVLFVTLEQVRKLLKDF