Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012793.t1 | XP_013458716.1 | 91.6 | 706 | 22 | 1 | 1 | 669 | 1 | 706 | 0.00E+00 | 1112.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012793.t1 | Q9LTY1 | 56.9 | 726 | 254 | 7 | 1 | 668 | 1 | 725 | 8.9e-199 | 694.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012793.t1 | A0A072UUK2 | 91.6 | 706 | 22 | 1 | 1 | 669 | 1 | 706 | 0.0e+00 | 1112.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067039 | MS.gene012793 | PPI |
| MS.gene012793 | MS.gene75567 | PPI |
| MS.gene012793 | MS.gene021252 | PPI |
| MS.gene78111 | MS.gene012793 | PPI |
| MS.gene012793 | MS.gene003211 | PPI |
| MS.gene012793 | MS.gene03802 | PPI |
| MS.gene66800 | MS.gene012793 | PPI |
| MS.gene012793 | MS.gene79126 | PPI |
| MS.gene045227 | MS.gene012793 | PPI |
| MS.gene012793 | MS.gene02806 | PPI |
| MS.gene012793 | MS.gene02011 | PPI |
| MS.gene012793 | MS.gene99360 | PPI |
| MS.gene012793 | MS.gene02013 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012793.t1 | MTR_4g134640 | 91.643 | 706 | 22 | 1 | 1 | 669 | 1 | 706 | 0.0 | 1292 |
| MS.gene012793.t1 | MTR_4g134640 | 96.790 | 623 | 20 | 0 | 47 | 669 | 4 | 626 | 0.0 | 1209 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012793.t1 | AT5G49880 | 59.366 | 726 | 236 | 7 | 1 | 668 | 1 | 725 | 0.0 | 811 |
Find 131 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCACTCTCAGCTCATTTA+AGG | 0.127080 | 8.1:-223656 | None:intergenic |
| AGCTTCACCATTCTTCAAAT+TGG | 0.225489 | 8.1:-223458 | None:intergenic |
| ATCAGGGAATACCAACATTT+TGG | 0.238178 | 8.1:+226222 | MS.gene012793:CDS |
| TGCGAAGGTTTGTCTCATTT+TGG | 0.238880 | 8.1:-220012 | None:intergenic |
| TTCTACAGGGAGTGATGTTT+TGG | 0.261658 | 8.1:+220546 | MS.gene012793:CDS |
| CAGCACTTCTATGGTTTGTT+TGG | 0.266549 | 8.1:-224708 | None:intergenic |
| TGATCATTCACAACTTAAAA+AGG | 0.271940 | 8.1:+220144 | MS.gene012793:CDS |
| CTTGAATTTGAATATGAATC+AGG | 0.287905 | 8.1:+226205 | MS.gene012793:CDS |
| TTGAACTGTTTAGGAGAAAC+TGG | 0.306116 | 8.1:+225394 | MS.gene012793:intron |
| TTCAGAGATATCATTATTAG+AGG | 0.326892 | 8.1:+223777 | MS.gene012793:CDS |
| GACAAAGCAACATCTTTAGA+AGG | 0.333909 | 8.1:+220067 | MS.gene012793:CDS |
| CTAAAGATGTTGCTTTGTCT+TGG | 0.341234 | 8.1:-220063 | None:intergenic |
| GAAACAACTGGAGGTGGCTT+TGG | 0.341324 | 8.1:+222567 | MS.gene012793:CDS |
| CATTGCAAACCTCTTTGAAT+TGG | 0.351624 | 8.1:-220361 | None:intergenic |
| TCAATTAAACACTGAACTTC+AGG | 0.351637 | 8.1:+219982 | MS.gene012793:CDS |
| GCTCAGATTGTGCCCTTTCC+CGG | 0.351664 | 8.1:-221845 | None:intergenic |
| TCCCAGGAAGTTTCTTCTAC+AGG | 0.379616 | 8.1:+220532 | MS.gene012793:CDS |
| TATTAAAATCAGAGATTAAA+CGG | 0.384162 | 8.1:+222656 | MS.gene012793:CDS |
| AACTGCTCGCTTGAAACAAC+TGG | 0.389194 | 8.1:+222555 | MS.gene012793:CDS |
| ATTCTTGCCAATTTGAAGAA+TGG | 0.390364 | 8.1:+223451 | MS.gene012793:CDS |
| TAAAGATGTTGCTTTGTCTT+GGG | 0.400406 | 8.1:-220062 | None:intergenic |
| TCCCTGTAGAAGAAACTTCC+TGG | 0.401242 | 8.1:-220534 | None:intergenic |
| CTTTGAATTGGTCCTTAAGA+TGG | 0.406182 | 8.1:-220349 | None:intergenic |
| ACATTGACAACTTACATTAG+TGG | 0.415112 | 8.1:-220194 | None:intergenic |
| AGACCTGCTTAAAGAGCAAC+TGG | 0.415270 | 8.1:+220325 | MS.gene012793:CDS |
| GATTAAACGGATTGAATTAA+CGG | 0.416637 | 8.1:+222669 | MS.gene012793:CDS |
| TCATCGGAACGTGGCCTCTT+CGG | 0.417235 | 8.1:-219450 | None:intergenic |
| TGCTTTGTCTTGGGCGGAAG+CGG | 0.418194 | 8.1:-220053 | None:intergenic |
| TTTGGTTAAACAATTGCAAC+AGG | 0.422570 | 8.1:+220564 | MS.gene012793:CDS |
| GATTCTAGAAATAGAAAAGT+TGG | 0.428700 | 8.1:-220170 | None:intergenic |
| AAGCAGGTCTGTTTCTCTCT+CGG | 0.431745 | 8.1:-220312 | None:intergenic |
| AGAGCAACTGGACCATCTTA+AGG | 0.435575 | 8.1:+220337 | MS.gene012793:CDS |
| GCTTTGGATGCTGCTGAGAT+TGG | 0.436789 | 8.1:+222583 | MS.gene012793:CDS |
| GATGAACACCAGCGATCAAA+TGG | 0.439362 | 8.1:+226124 | MS.gene012793:CDS |
| TGCGTCGCCGTCGCCGTCAT+CGG | 0.440741 | 8.1:-219466 | None:intergenic |
| TGCAGCGTAAATCGTGTCAC+TGG | 0.446319 | 8.1:-226151 | None:intergenic |
| TGCTGAAACTGAAGCTGTGC+TGG | 0.451059 | 8.1:+222615 | MS.gene012793:CDS |
| TTGTTTGGCTTCATTATCTA+CGG | 0.458408 | 8.1:-224693 | None:intergenic |
| GGATCCGGCGGCAGCGGTGG+AGG | 0.459538 | 8.1:+219506 | MS.gene012793:CDS |
| GAAGAATTGAAGAGTCAATC+AGG | 0.460323 | 8.1:+224773 | MS.gene012793:CDS |
| AAGTAGTGCTAATCCCATTC+AGG | 0.466808 | 8.1:+223492 | MS.gene012793:CDS |
| GGAAAAGAGCCGCCGGGAAA+GGG | 0.468010 | 8.1:+221833 | MS.gene012793:CDS |
| GAGGGAAATACAACCTGAAT+GGG | 0.468113 | 8.1:-223505 | None:intergenic |
| CTGGGATTCCATTTGATCGC+TGG | 0.470960 | 8.1:-226132 | None:intergenic |
| CAAGAACTCGCTGCTTCTAA+AGG | 0.472308 | 8.1:+219887 | MS.gene012793:CDS |
| TCTTCGGTGGCGGAGGAGTA+CGG | 0.473231 | 8.1:-219434 | None:intergenic |
| CGTGGCCTCTTCGGTGGCGG+AGG | 0.474010 | 8.1:-219441 | None:intergenic |
| ACTCCAGGATCAACTCTTGA+AGG | 0.475492 | 8.1:+219922 | MS.gene012793:CDS |
| CACTGGAGAGGGAGAGTGAC+CGG | 0.480225 | 8.1:+223749 | MS.gene012793:CDS |
| TGTAAAGCTGCAAATTTAGC+AGG | 0.480888 | 8.1:-221978 | None:intergenic |
| AGAGGGAAATACAACCTGAA+TGG | 0.481847 | 8.1:-223506 | None:intergenic |
| CTATGAGGATCCGGCGGCAG+CGG | 0.485300 | 8.1:+219500 | MS.gene012793:CDS |
| TCAGCCTGAGGTATCTCGTC+AGG | 0.487574 | 8.1:+226441 | MS.gene012793:CDS |
| AAGAATAAAGTCACTGGAGA+GGG | 0.490630 | 8.1:+223738 | MS.gene012793:CDS |
| TGCTTCTAAAGGCCGTGAAA+AGG | 0.491539 | 8.1:+219898 | MS.gene012793:CDS |
| CCCAGGTTGAGATATTCGTT+CGG | 0.491573 | 8.1:+227562 | MS.gene012793:intron |
| GGTTCCTCCACCGCTGCCGC+CGG | 0.494163 | 8.1:-219510 | None:intergenic |
| ATCTGAGCTATCTAAACTAC+AGG | 0.495023 | 8.1:+221860 | MS.gene012793:CDS |
| TGAGGATCCGGCGGCAGCGG+TGG | 0.495529 | 8.1:+219503 | MS.gene012793:CDS |
| GGTCCAGTTGCTCTTTAAGC+AGG | 0.500008 | 8.1:-220328 | None:intergenic |
| AGGAAAAGAGCCGCCGGGAA+AGG | 0.500081 | 8.1:+221832 | MS.gene012793:CDS |
| GTATCTTAATTTCCTCATGT+TGG | 0.507329 | 8.1:-223687 | None:intergenic |
| CCCAGGAAGTTTCTTCTACA+GGG | 0.507888 | 8.1:+220533 | MS.gene012793:CDS |
| GAACGTGGCCTCTTCGGTGG+CGG | 0.508356 | 8.1:-219444 | None:intergenic |
| GATATCATTATTAGAGGCAA+AGG | 0.509270 | 8.1:+223783 | MS.gene012793:CDS |
| GCTTGTTATCTATGAGGATC+CGG | 0.510386 | 8.1:+219491 | MS.gene012793:CDS |
| AGGCCGTGAAAAGGCACTCC+AGG | 0.512926 | 8.1:+219907 | MS.gene012793:CDS |
| TTCCTTCTACAGTTAGGTCA+TGG | 0.513722 | 8.1:+224620 | MS.gene012793:intron |
| AAATCTCACAGCACGCCCTC+CGG | 0.515186 | 8.1:-225624 | None:intergenic |
| TCAACATCTTACCAAAATGT+TGG | 0.516170 | 8.1:-226233 | None:intergenic |
| TTGAATTTGAATATGAATCA+GGG | 0.517570 | 8.1:+226206 | MS.gene012793:CDS |
| GAAAGACTCCACTGTTATGT+TGG | 0.523713 | 8.1:-227613 | None:intergenic |
| TGCTGGAGGAAAAGAGCCGC+CGG | 0.524219 | 8.1:+221826 | MS.gene012793:CDS |
| TTTAGGAGAAACTGGAAAGC+TGG | 0.527669 | 8.1:+225402 | MS.gene012793:intron |
| TCTCTATGGTCTCTGAAAGA+TGG | 0.530121 | 8.1:-220099 | None:intergenic |
| GGCGGAAGCGGCATGAGACT+CGG | 0.530624 | 8.1:-220041 | None:intergenic |
| GTTATGTTGGCAGTAAAAGC+AGG | 0.531953 | 8.1:-227600 | None:intergenic |
| GCTGGTGGAGAGCTACATAT+CGG | 0.532673 | 8.1:+225420 | MS.gene012793:CDS |
| AGCAAGAAGAATAAAGTCAC+TGG | 0.533486 | 8.1:+223732 | MS.gene012793:CDS |
| CGCACAGCTTGTTATCTATG+AGG | 0.533714 | 8.1:+219485 | MS.gene012793:CDS |
| ACCTGGCATCTCATGTTTCG+AGG | 0.534847 | 8.1:+221950 | MS.gene012793:CDS |
| CCTCCTTCAAGAGTTGATCC+TGG | 0.535275 | 8.1:-219925 | None:intergenic |
| ATGATATCTCTGAACGGAGC+CGG | 0.537996 | 8.1:-223768 | None:intergenic |
| AAGAGGCCACGTTCCGATGA+CGG | 0.543240 | 8.1:+219453 | MS.gene012793:CDS |
| TCCTCGAAACATGAGATGCC+AGG | 0.545495 | 8.1:-221951 | None:intergenic |
| TGAAATTCTGTCTGCAAACA+CGG | 0.545580 | 8.1:-225596 | None:intergenic |
| AATTTGAAGAATGGTGAAGC+TGG | 0.552951 | 8.1:+223460 | MS.gene012793:CDS |
| TCGCTTGAAACAACTGGAGG+TGG | 0.553739 | 8.1:+222561 | MS.gene012793:CDS |
| CTATATTTGGCAGATTGTGA+TGG | 0.562845 | 8.1:+226102 | MS.gene012793:intron |
| CCCTGTAGAAGAAACTTCCT+GGG | 0.564281 | 8.1:-220533 | None:intergenic |
| TAGAGGATCAAATATCATCA+TGG | 0.572164 | 8.1:+221907 | MS.gene012793:CDS |
| ACATCTTTAGAAGGGAAGCT+AGG | 0.573337 | 8.1:+220076 | MS.gene012793:CDS |
| TCGGAACGTGGCCTCTTCGG+TGG | 0.574434 | 8.1:-219447 | None:intergenic |
| CTTACCTGACGAGATACCTC+AGG | 0.581003 | 8.1:-226445 | None:intergenic |
| GTGCATAGCATGTGCTCGGA+GGG | 0.581042 | 8.1:-219531 | None:intergenic |
| GTACTCCTCCGCCACCGAAG+AGG | 0.584759 | 8.1:+219436 | MS.gene012793:CDS |
| ACAAAGCAACATCTTTAGAA+GGG | 0.593342 | 8.1:+220068 | MS.gene012793:CDS |
| AATTTACAGTACACAGAAGG+AGG | 0.597563 | 8.1:+222525 | MS.gene012793:CDS |
| TCTTAAGGACCAATTCAAAG+AGG | 0.599055 | 8.1:+220352 | MS.gene012793:CDS |
| GGTAATTTACAGTACACAGA+AGG | 0.606067 | 8.1:+222522 | MS.gene012793:CDS |
| CCGTCGCCGTCATCGGAACG+TGG | 0.606794 | 8.1:-219459 | None:intergenic |
| CACCTTAAATGAGCTGAGAG+TGG | 0.608157 | 8.1:+223654 | MS.gene012793:CDS |
| GCAGCGTAAATCGTGTCACT+GGG | 0.613627 | 8.1:-226150 | None:intergenic |
| GAGACTCGGCTTGTATGCGA+AGG | 0.619176 | 8.1:-220027 | None:intergenic |
| GGTTAATAATCGCCAACATG+AGG | 0.626687 | 8.1:+223675 | MS.gene012793:CDS |
| CCACGTTCCGATGACGGCGA+CGG | 0.626939 | 8.1:+219459 | MS.gene012793:CDS |
| AGTCCATTAAATTTATGACA+AGG | 0.631335 | 8.1:-227646 | None:intergenic |
| AGATGTTGCTTTGTCTTGGG+CGG | 0.631632 | 8.1:-220059 | None:intergenic |
| GCTGGAGGAAAAGAGCCGCC+GGG | 0.633967 | 8.1:+221827 | MS.gene012793:CDS |
| GAAGAATAAAGTCACTGGAG+AGG | 0.635917 | 8.1:+223737 | MS.gene012793:CDS |
| AGTACACAGAAGGAGGGTGA+AGG | 0.638528 | 8.1:+222532 | MS.gene012793:CDS |
| CACTAGAGAAGCGAGAAGAG+AGG | 0.638861 | 8.1:+225476 | MS.gene012793:CDS |
| CTAATAATGATATCTCTGAA+CGG | 0.639682 | 8.1:-223774 | None:intergenic |
| TAATACAAAAGTTCTGCGGA+TGG | 0.640306 | 8.1:+224658 | MS.gene012793:CDS |
| AGGAGAAACTGGAAAGCTGG+TGG | 0.645216 | 8.1:+225405 | MS.gene012793:intron |
| ATATGTGCATAGCATGTGCT+CGG | 0.645443 | 8.1:-219535 | None:intergenic |
| CACTGAGAGGAAGAAATCTG+TGG | 0.647981 | 8.1:+219844 | MS.gene012793:CDS |
| TTTGAAGGAGAAACTGCTGG+AGG | 0.658729 | 8.1:+221812 | MS.gene012793:CDS |
| TGCTCGCTTGAAACAACTGG+AGG | 0.661475 | 8.1:+222558 | MS.gene012793:CDS |
| TGTGCATAGCATGTGCTCGG+AGG | 0.667920 | 8.1:-219532 | None:intergenic |
| TCTTTCAGAGACCATAGAGA+GGG | 0.673302 | 8.1:+220102 | MS.gene012793:CDS |
| ATGACATGACAGACACTGAG+AGG | 0.677214 | 8.1:+219831 | MS.gene012793:intron |
| TCATTACACATCTCAGCCTG+AGG | 0.681344 | 8.1:+226429 | MS.gene012793:CDS |
| CCAGGATCAACTCTTGAAGG+AGG | 0.683215 | 8.1:+219925 | MS.gene012793:CDS |
| CTGCTAATACAAAAGTTCTG+CGG | 0.683446 | 8.1:+224654 | MS.gene012793:CDS |
| GCAGCTAGCAGTTGTCACAG+AGG | 0.683964 | 8.1:+223411 | MS.gene012793:intron |
| ATTTACAGTACACAGAAGGA+GGG | 0.690417 | 8.1:+222526 | MS.gene012793:CDS |
| TGTTATCTATGAGGATCCGG+CGG | 0.704459 | 8.1:+219494 | MS.gene012793:CDS |
| CAGATTGTGCCCTTTCCCGG+CGG | 0.704598 | 8.1:-221842 | None:intergenic |
| ATCTTTCAGAGACCATAGAG+AGG | 0.704981 | 8.1:+220101 | MS.gene012793:CDS |
| TTGATGATTAAAGACATACC+TGG | 0.713365 | 8.1:+221933 | MS.gene012793:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 219426 | 227656 | 219426 | ID=MS.gene012793 |
| chr8.1 | mRNA | 219426 | 227656 | 219426 | ID=MS.gene012793.t1;Parent=MS.gene012793 |
| chr8.1 | exon | 219426 | 219561 | 219426 | ID=MS.gene012793.t1.exon1;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 219426 | 219561 | 219426 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 219843 | 220201 | 219843 | ID=MS.gene012793.t1.exon2;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 219843 | 220201 | 219843 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 220281 | 220373 | 220281 | ID=MS.gene012793.t1.exon3;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 220281 | 220373 | 220281 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 220484 | 220600 | 220484 | ID=MS.gene012793.t1.exon4;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 220484 | 220600 | 220484 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 221756 | 222003 | 221756 | ID=MS.gene012793.t1.exon5;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 221756 | 222003 | 221756 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 222519 | 222690 | 222519 | ID=MS.gene012793.t1.exon6;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 222519 | 222690 | 222519 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 223415 | 223513 | 223415 | ID=MS.gene012793.t1.exon7;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 223415 | 223513 | 223415 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 223601 | 223804 | 223601 | ID=MS.gene012793.t1.exon8;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 223601 | 223804 | 223601 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 224632 | 224794 | 224632 | ID=MS.gene012793.t1.exon9;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 224632 | 224794 | 224632 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 225407 | 225497 | 225407 | ID=MS.gene012793.t1.exon10;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 225407 | 225497 | 225407 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 225588 | 225657 | 225588 | ID=MS.gene012793.t1.exon11;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 225588 | 225657 | 225588 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 226115 | 226243 | 226115 | ID=MS.gene012793.t1.exon12;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 226115 | 226243 | 226115 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 226424 | 226462 | 226424 | ID=MS.gene012793.t1.exon13;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 226424 | 226462 | 226424 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| chr8.1 | exon | 227567 | 227656 | 227567 | ID=MS.gene012793.t1.exon14;Parent=MS.gene012793.t1 |
| chr8.1 | CDS | 227567 | 227656 | 227567 | ID=cds.MS.gene012793.t1;Parent=MS.gene012793.t1 |
| Gene Sequence |
| Protein sequence |
