Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021252.t1 | XP_003594432.1 | 68.6 | 491 | 54 | 3 | 32 | 422 | 85 | 575 | 2.20E-162 | 582 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021252.t1 | Q9M1G5 | 31.4 | 472 | 221 | 7 | 33 | 404 | 94 | 562 | 3.8e-54 | 213.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021252.t1 | G7IFT2 | 68.6 | 491 | 54 | 3 | 32 | 422 | 85 | 575 | 1.6e-162 | 582.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene016667 | MS.gene021252 | PPI |
| MS.gene95049 | MS.gene021252 | PPI |
| MS.gene43480 | MS.gene021252 | PPI |
| MS.gene012793 | MS.gene021252 | PPI |
| MS.gene79126 | MS.gene021252 | PPI |
| MS.gene95250 | MS.gene021252 | PPI |
| MS.gene042046 | MS.gene021252 | PPI |
| MS.gene067039 | MS.gene021252 | PPI |
| MS.gene056169 | MS.gene021252 | PPI |
| MS.gene75567 | MS.gene021252 | PPI |
| MS.gene45748 | MS.gene021252 | PPI |
| MS.gene036083 | MS.gene021252 | PPI |
| MS.gene045227 | MS.gene021252 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021252.t1 | MTR_2g028550 | 72.301 | 491 | 36 | 3 | 32 | 422 | 85 | 575 | 0.0 | 658 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021252.t1 | AT3G54630 | 34.820 | 471 | 206 | 5 | 33 | 404 | 94 | 562 | 1.50e-85 | 272 |
Find 98 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAAATTCGGTGGTGAAAT+TGG | 0.216989 | 6.1:+44332363 | MS.gene021252:CDS |
| CAATGCTTTGTCTTATTCAT+TGG | 0.263535 | 6.1:+44331250 | MS.gene021252:CDS |
| TATAAGATAATTGAGGAGTT+TGG | 0.272199 | 6.1:+44331631 | MS.gene021252:CDS |
| CTATAGTTTCAGAGGTTAGA+AGG | 0.303946 | 6.1:+44360824 | MS.gene021252:CDS |
| TGAGCCGTTGGAGAGGGTTT+TGG | 0.311584 | 6.1:+44331663 | MS.gene021252:CDS |
| TTCAGCAGGTGTGGCTCCCT+TGG | 0.329571 | 6.1:-44332275 | None:intergenic |
| TAAGAGAAAAGTGGAGTTGC+AGG | 0.331677 | 6.1:+44331756 | MS.gene021252:CDS |
| AACGGGGAATTCAAGGAGTT+TGG | 0.339763 | 6.1:-44331121 | None:intergenic |
| ATCTGTTGCTGAGGAGAAAC+TGG | 0.341692 | 6.1:+44331486 | MS.gene021252:CDS |
| TGTGCTTCTAGGTTGAATAT+TGG | 0.344319 | 6.1:+44332226 | MS.gene021252:intron |
| GCATAAGATCAAAGACTTAG+AGG | 0.380540 | 6.1:+44331861 | MS.gene021252:CDS |
| GGCCATTGATGCGGAGTAGC+AGG | 0.380994 | 6.1:-44331225 | None:intergenic |
| AGGATGAGCTTGAGAAAGAA+AGG | 0.397850 | 6.1:+44331578 | MS.gene021252:CDS |
| GGCATCTGAATTGAAGTTGC+AGG | 0.406818 | 6.1:+44360705 | MS.gene021252:intron |
| GAAAATTCTGCTAGGCTTGA+AGG | 0.407337 | 6.1:+44332418 | MS.gene021252:CDS |
| TGAATAAGACAAAGCATTGA+AGG | 0.417031 | 6.1:-44331246 | None:intergenic |
| TGAGAAAGAAAGGGGTGTGT+TGG | 0.420784 | 6.1:+44331588 | MS.gene021252:CDS |
| TCAGCAGGTGTGGCTCCCTT+GGG | 0.420953 | 6.1:-44332274 | None:intergenic |
| GAGAAAGAAAGGGGTGTGTT+GGG | 0.423854 | 6.1:+44331589 | MS.gene021252:CDS |
| AGCCACACCTGCTGAAATCA+TGG | 0.428974 | 6.1:+44332282 | MS.gene021252:CDS |
| TGACGAGGCTATTCTTGATA+TGG | 0.429407 | 6.1:+44331429 | MS.gene021252:CDS |
| ATTGATTTATTAAGCTTGAA+AGG | 0.433644 | 6.1:-44331192 | None:intergenic |
| TGTTGAGTTGGAGGCGCAAT+TGG | 0.435358 | 6.1:+44331528 | MS.gene021252:CDS |
| CTAGTGAGGCGGAACTTACT+AGG | 0.442397 | 6.1:+44331794 | MS.gene021252:CDS |
| CGGAACTTACTAGGAATGCT+TGG | 0.445936 | 6.1:+44331803 | MS.gene021252:CDS |
| TGCGATTGCGGATTGTTGAT+AGG | 0.448386 | 6.1:-44331015 | None:intergenic |
| GTTTGTTAACGGGGAATTCA+AGG | 0.451050 | 6.1:-44331128 | None:intergenic |
| GGAGAAGCAGTTGGAGGCTA+AGG | 0.456175 | 6.1:+44331693 | MS.gene021252:CDS |
| TTCGTCTTCGAGTTTGTTAA+CGG | 0.457672 | 6.1:-44331139 | None:intergenic |
| TTGATTTATTAAGCTTGAAA+GGG | 0.470084 | 6.1:-44331191 | None:intergenic |
| AGAGGCGAGGAAGGAGTTGA+TGG | 0.475835 | 6.1:-44331039 | None:intergenic |
| TCGTCTTCGAGTTTGTTAAC+GGG | 0.477823 | 6.1:-44331138 | None:intergenic |
| GGTGGTGAAATTGGAAGAGT+TGG | 0.481970 | 6.1:+44332372 | MS.gene021252:CDS |
| TGCTTGGGAGGAAAAGTGTT+GGG | 0.483018 | 6.1:+44331819 | MS.gene021252:CDS |
| TGAGGAGATTAAGAGAAAAG+TGG | 0.483684 | 6.1:+44331747 | MS.gene021252:CDS |
| GGATGAGCTTGAGAAAGAAA+GGG | 0.484285 | 6.1:+44331579 | MS.gene021252:CDS |
| ACCCTGCTACTCCGCATCAA+TGG | 0.496718 | 6.1:+44331223 | MS.gene021252:CDS |
| AAATTAGGGTTTCGTGGGGA+AGG | 0.497630 | 6.1:-44331092 | None:intergenic |
| AAAAGAACCTCCTACGAGCC+TGG | 0.500931 | 6.1:-44331903 | None:intergenic |
| TGAGCAAACTGTTGTTGAGT+TGG | 0.501346 | 6.1:+44331516 | MS.gene021252:CDS |
| TCAGCATATGATTCAAGTAC+AGG | 0.501972 | 6.1:-44332331 | None:intergenic |
| TGATGACATCAAGAGAAATT+CGG | 0.502763 | 6.1:+44332351 | MS.gene021252:CDS |
| GCAAACTGTTGTTGAGTTGG+AGG | 0.509033 | 6.1:+44331519 | MS.gene021252:CDS |
| AAGGATTGTCGAGGAGAATG+AGG | 0.510239 | 6.1:+44331729 | MS.gene021252:CDS |
| GAGATCAGTGCAGTCTAAGA+AGG | 0.510410 | 6.1:+44331558 | MS.gene021252:CDS |
| GCAGTTGGAGGCTAAGGTGG+TGG | 0.516624 | 6.1:+44331699 | MS.gene021252:CDS |
| GGCGGAGAAGGAGAAGCAGT+TGG | 0.516817 | 6.1:+44331684 | MS.gene021252:CDS |
| TCAGTACGTGTTGGATCCCA+AGG | 0.521099 | 6.1:+44332258 | MS.gene021252:CDS |
| TGAGTTGGAGGCGCAATTGG+AGG | 0.523011 | 6.1:+44331531 | MS.gene021252:CDS |
| CTCAAAGTATAAAGAACATG+TGG | 0.527765 | 6.1:+44360798 | MS.gene021252:CDS |
| AAAATATAGTTATGGATTGG+AGG | 0.527971 | 6.1:+44360903 | MS.gene021252:CDS |
| ACGAGCCTGGTTGCAATCGA+TGG | 0.534197 | 6.1:-44331890 | None:intergenic |
| TCAAACAGTTTAAGAAGTAG+TGG | 0.534595 | 6.1:-44331165 | None:intergenic |
| GGAACTTACTAGGAATGCTT+GGG | 0.534865 | 6.1:+44331804 | MS.gene021252:CDS |
| AAAATTCTGCTAGGCTTGAA+GGG | 0.537545 | 6.1:+44332419 | MS.gene021252:CDS |
| TCAATACCCATGATTTCAGC+AGG | 0.541735 | 6.1:-44332289 | None:intergenic |
| GGAGTTGATGGTTGCGATTG+CGG | 0.543181 | 6.1:-44331027 | None:intergenic |
| ATGCTTGGGAGGAAAAGTGT+TGG | 0.549756 | 6.1:+44331818 | MS.gene021252:CDS |
| GAGAATTGAGCCGTTGGAGA+GGG | 0.549810 | 6.1:+44331657 | MS.gene021252:CDS |
| GTTGGAGGCGCAATTGGAGG+AGG | 0.550019 | 6.1:+44331534 | MS.gene021252:CDS |
| TGGATCGAGAATTGAGCCGT+TGG | 0.551014 | 6.1:+44331651 | MS.gene021252:CDS |
| CTAAGGTGGTGGAGAATGAA+AGG | 0.551554 | 6.1:+44331710 | MS.gene021252:CDS |
| GAAACTGTGAGAGGCGAGGA+AGG | 0.553109 | 6.1:-44331048 | None:intergenic |
| GAGAAGATTTGAAAGAGATG+TGG | 0.554044 | 6.1:-44331070 | None:intergenic |
| TATAGTTTCAGAGGTTAGAA+GGG | 0.555223 | 6.1:+44360825 | MS.gene021252:CDS |
| GAAGCAGTTGGAGGCTAAGG+TGG | 0.568442 | 6.1:+44331696 | MS.gene021252:CDS |
| CGAGAATTGAGCCGTTGGAG+AGG | 0.570516 | 6.1:+44331656 | MS.gene021252:CDS |
| ACCCATGATTTCAGCAGGTG+TGG | 0.571033 | 6.1:-44332284 | None:intergenic |
| TAATGACATTCAGTACGTGT+TGG | 0.573629 | 6.1:+44332249 | MS.gene021252:CDS |
| TCATGAGAAATCTGTTGCTG+AGG | 0.575318 | 6.1:+44331477 | MS.gene021252:CDS |
| TCTCTCCATCGATTGCAACC+AGG | 0.578773 | 6.1:+44331885 | MS.gene021252:CDS |
| CGAGGCTATTCTTGATATGG+AGG | 0.579105 | 6.1:+44331432 | MS.gene021252:CDS |
| CGTCTTCGAGTTTGTTAACG+GGG | 0.583351 | 6.1:-44331137 | None:intergenic |
| GGAGAAGGAGAAGCAGTTGG+AGG | 0.585972 | 6.1:+44331687 | MS.gene021252:CDS |
| GCCATTGATGCGGAGTAGCA+GGG | 0.589115 | 6.1:-44331224 | None:intergenic |
| TGTGGGATCTATAGTTTCAG+AGG | 0.589958 | 6.1:+44360816 | MS.gene021252:CDS |
| TGCTGAGAGAGATGCTAGTG+AGG | 0.591450 | 6.1:+44331780 | MS.gene021252:CDS |
| AATCAACTTAGAAAGTTCAC+CGG | 0.599947 | 6.1:-44360767 | None:intergenic |
| GATGAGCTTGAGAAAGAAAG+GGG | 0.600316 | 6.1:+44331580 | MS.gene021252:CDS |
| TTGCGGATTGTTGATAGGAA+CGG | 0.607890 | 6.1:-44331010 | None:intergenic |
| ACTTACTAGGAATGCTTGGG+AGG | 0.610350 | 6.1:+44331807 | MS.gene021252:CDS |
| AGTCTGCTGAAAATTCTGCT+AGG | 0.616077 | 6.1:+44332410 | MS.gene021252:CDS |
| TCGATTGCAACCAGGCTCGT+AGG | 0.620427 | 6.1:+44331893 | MS.gene021252:CDS |
| ATTGCAACCAGGCTCGTAGG+AGG | 0.620797 | 6.1:+44331896 | MS.gene021252:CDS |
| ATAGTTTCAGAGGTTAGAAG+GGG | 0.620828 | 6.1:+44360826 | MS.gene021252:CDS |
| GCCACACCTGCTGAAATCAT+GGG | 0.621915 | 6.1:+44332283 | MS.gene021252:CDS |
| AAAGAAAGGGGTGTGTTGGG+AGG | 0.622863 | 6.1:+44331592 | MS.gene021252:CDS |
| AGCATTGAAGGCCATTGATG+CGG | 0.625810 | 6.1:-44331234 | None:intergenic |
| TCAAAGTATAAAGAACATGT+GGG | 0.633300 | 6.1:+44360799 | MS.gene021252:CDS |
| GGAGAATGAAAGGATTGTCG+AGG | 0.645887 | 6.1:+44331720 | MS.gene021252:CDS |
| TCCGCCAAAACCCTCTCCAA+CGG | 0.655375 | 6.1:-44331667 | None:intergenic |
| GAAGAAATTCAAATGCATGC+CGG | 0.655574 | 6.1:+44360748 | MS.gene021252:CDS |
| TGTGGAAACTGTGAGAGGCG+AGG | 0.661863 | 6.1:-44331052 | None:intergenic |
| TGAGAGAGATGCTAGTGAGG+CGG | 0.680560 | 6.1:+44331783 | MS.gene021252:CDS |
| CAGTACGTGTTGGATCCCAA+GGG | 0.692879 | 6.1:+44332259 | MS.gene021252:CDS |
| TGACATCAAGAGAAATTCGG+TGG | 0.701910 | 6.1:+44332354 | MS.gene021252:CDS |
| TAGTTTCAGAGGTTAGAAGG+GGG | 0.727851 | 6.1:+44360827 | MS.gene021252:CDS |
| AGAGATGTGGAAACTGTGAG+AGG | 0.737547 | 6.1:-44331057 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 44331006 | 44360954 | 44331006 | ID=MS.gene021252 |
| chr6.1 | mRNA | 44331006 | 44360954 | 44331006 | ID=MS.gene021252.t1;Parent=MS.gene021252 |
| chr6.1 | exon | 44331006 | 44331285 | 44331006 | ID=MS.gene021252.t1.exon1;Parent=MS.gene021252.t1 |
| chr6.1 | CDS | 44331006 | 44331285 | 44331006 | ID=cds.MS.gene021252.t1;Parent=MS.gene021252.t1 |
| chr6.1 | exon | 44331425 | 44331917 | 44331425 | ID=MS.gene021252.t1.exon2;Parent=MS.gene021252.t1 |
| chr6.1 | CDS | 44331425 | 44331917 | 44331425 | ID=cds.MS.gene021252.t1;Parent=MS.gene021252.t1 |
| chr6.1 | exon | 44332237 | 44332483 | 44332237 | ID=MS.gene021252.t1.exon3;Parent=MS.gene021252.t1 |
| chr6.1 | CDS | 44332237 | 44332483 | 44332237 | ID=cds.MS.gene021252.t1;Parent=MS.gene021252.t1 |
| chr6.1 | exon | 44360706 | 44360954 | 44360706 | ID=MS.gene021252.t1.exon4;Parent=MS.gene021252.t1 |
| chr6.1 | CDS | 44360706 | 44360954 | 44360706 | ID=cds.MS.gene021252.t1;Parent=MS.gene021252.t1 |
| Gene Sequence |
| Protein sequence |
