Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015741.t1 | AET05287.1 | 92.7 | 151 | 11 | 0 | 2 | 152 | 63 | 213 | 4.00E-74 | 287.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015741.t1 | Q54UU1 | 54.4 | 125 | 56 | 1 | 15 | 138 | 3 | 127 | 3.2e-35 | 149.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015741.t1 | G7L8Y7 | 92.7 | 151 | 11 | 0 | 2 | 152 | 63 | 213 | 2.9e-74 | 287.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene96826 | MS.gene015741 | PPI |
| MS.gene59549 | MS.gene015741 | PPI |
| MS.gene031550 | MS.gene015741 | PPI |
| MS.gene006753 | MS.gene015741 | PPI |
| MS.gene85984 | MS.gene015741 | PPI |
| MS.gene068341 | MS.gene015741 | PPI |
| MS.gene015741 | MS.gene87621 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015741.t1 | MTR_8g103680 | 92.715 | 151 | 11 | 0 | 2 | 152 | 63 | 213 | 5.57e-105 | 298 |
| MS.gene015741.t1 | MTR_5g075330 | 87.333 | 150 | 19 | 0 | 3 | 152 | 25 | 174 | 3.74e-101 | 287 |
| MS.gene015741.t1 | MTR_5g075330 | 90.071 | 141 | 14 | 0 | 12 | 152 | 1 | 141 | 3.63e-98 | 279 |
| MS.gene015741.t1 | MTR_8g095450 | 28.873 | 142 | 84 | 3 | 18 | 143 | 28 | 168 | 1.03e-14 | 67.8 |
| MS.gene015741.t1 | MTR_4g048530 | 29.787 | 141 | 84 | 3 | 18 | 143 | 26 | 166 | 1.48e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015741.t1 | AT5G02280 | 87.943 | 141 | 17 | 0 | 12 | 152 | 1 | 141 | 4.05e-94 | 268 |
| MS.gene015741.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 5.86e-14 | 65.9 |
| MS.gene015741.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 5.86e-14 | 65.9 |
| MS.gene015741.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 5.86e-14 | 65.9 |
Find 35 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCAGTTGTCACCTGTTTC+TGG | 0.050534 | 4.3:+2043580 | MS.gene015741:CDS |
| TTTCCTGATATTTAGGATTA+TGG | 0.113471 | 4.3:+2043484 | MS.gene015741:intron |
| ATTTAGGATTATGGGTCTTC+TGG | 0.243224 | 4.3:+2043493 | MS.gene015741:intron |
| CTTTCCATTTGTTGAGTTCC+AGG | 0.279445 | 4.3:-2044182 | None:intergenic |
| TTCCTGATATTTAGGATTAT+GGG | 0.283975 | 4.3:+2043485 | MS.gene015741:intron |
| CAGATTCTTGCAAAGGCTTT+GGG | 0.307507 | 4.3:+2042798 | MS.gene015741:CDS |
| TCAGATTCTTGCAAAGGCTT+TGG | 0.323137 | 4.3:+2042797 | MS.gene015741:CDS |
| GAAGGACCGTGTTGCATTGC+TGG | 0.349606 | 4.3:+2044322 | MS.gene015741:CDS |
| CGGTGGATTGATCTACTATA+AGG | 0.397918 | 4.3:+2042860 | MS.gene015741:CDS |
| AGGCATCTCCATCTCATAGA+AGG | 0.407862 | 4.3:-2044255 | None:intergenic |
| GCTTACGGGTGGCTAGCTTA+TGG | 0.427608 | 4.3:+2043536 | MS.gene015741:CDS |
| AAATAATACCTGTCAGTGAC+TGG | 0.443726 | 4.3:-2043654 | None:intergenic |
| ATTCCTGCGCAACCAGAAAC+AGG | 0.450165 | 4.3:-2043592 | None:intergenic |
| TGAACCTGGAACTCAACAAA+TGG | 0.475337 | 4.3:+2044178 | MS.gene015741:CDS |
| TCAAAGAGCTCACATCGTAT+AGG | 0.480843 | 4.3:-2044275 | None:intergenic |
| GGACACCAATGATAGCTTAC+GGG | 0.481121 | 4.3:+2043522 | MS.gene015741:CDS |
| CATTGCTTCCAGTCACTGAC+AGG | 0.492920 | 4.3:+2043646 | MS.gene015741:CDS |
| GGCATCTCCATCTCATAGAA+GGG | 0.506472 | 4.3:-2044254 | None:intergenic |
| GGCATTTCAGATTCTTGCAA+AGG | 0.507176 | 4.3:+2042791 | MS.gene015741:CDS |
| AGCCACCCGTAAGCTATCAT+TGG | 0.510288 | 4.3:-2043527 | None:intergenic |
| TGGACACCAATGATAGCTTA+CGG | 0.512682 | 4.3:+2043521 | MS.gene015741:CDS |
| TCACCTGTTTCTGGTTGCGC+AGG | 0.516213 | 4.3:+2043589 | MS.gene015741:CDS |
| CTTTGATATCAACTTAGCTC+AGG | 0.519060 | 4.3:+2044292 | MS.gene015741:CDS |
| AAGGACCGTGTTGCATTGCT+GGG | 0.527169 | 4.3:+2044323 | MS.gene015741:CDS |
| GAAGATCAAATGTGTCAGCT+TGG | 0.530412 | 4.3:-2043624 | None:intergenic |
| CTTTACATCATCAATAAGTC+CGG | 0.534062 | 4.3:+2042840 | MS.gene015741:CDS |
| CTTAGCTCAGGCAGTGCAGA+AGG | 0.554879 | 4.3:+2044304 | MS.gene015741:CDS |
| TTATGGGTCTTCTGGACGAA+TGG | 0.565210 | 4.3:+2043501 | MS.gene015741:CDS |
| GAAGAACCCCTTCTATGAGA+TGG | 0.572651 | 4.3:+2044247 | MS.gene015741:CDS |
| TACATCATCAATAAGTCCGG+TGG | 0.589559 | 4.3:+2042843 | MS.gene015741:CDS |
| AGGTGACAACTGCTGAGAGA+TGG | 0.604194 | 4.3:-2043572 | None:intergenic |
| GCATCTCCATCTCATAGAAG+GGG | 0.643808 | 4.3:-2044253 | None:intergenic |
| CACCAATGATAGCTTACGGG+TGG | 0.665422 | 4.3:+2043525 | MS.gene015741:CDS |
| CTTATAGTAGATCAATCCAC+CGG | 0.673585 | 4.3:-2042859 | None:intergenic |
| ATCGACCCAGCAATGCAACA+CGG | 0.698434 | 4.3:-2044328 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAACAATACTAAAAAATTT+AGG | + | chr4.3:2043109-2043128 | MS.gene015741:intron | 10.0% |
| !! | GAAAGTTTAGTAATAATTAT+TGG | + | chr4.3:2043875-2043894 | MS.gene015741:intron | 15.0% |
| !!! | TTATTTTTATTGTAAGCTTA+AGG | - | chr4.3:2043220-2043239 | None:intergenic | 15.0% |
| !! | CTTAACATAATAATGAGTTA+TGG | - | chr4.3:2043681-2043700 | None:intergenic | 20.0% |
| !!! | TGTTTAATTTCCTGATATTT+AGG | + | chr4.3:2043477-2043496 | MS.gene015741:intron | 20.0% |
| !!! | TTCAGTAAAACATTTTTCAT+AGG | - | chr4.3:2042957-2042976 | None:intergenic | 20.0% |
| ! | AAAAACTTTCTGAGTTAGAT+GGG | - | chr4.3:2043384-2043403 | None:intergenic | 25.0% |
| ! | GGTAAGAATTCAAACATAAT+TGG | - | chr4.3:2043982-2044001 | None:intergenic | 25.0% |
| ! | TAAAAACTTTCTGAGTTAGA+TGG | - | chr4.3:2043385-2043404 | None:intergenic | 25.0% |
| ! | TGTGAATAAAAAGCAAACAT+TGG | - | chr4.3:2043782-2043801 | None:intergenic | 25.0% |
| !! | AAAGTTTTTACTACTGTTGT+TGG | + | chr4.3:2043395-2043414 | MS.gene015741:intron | 25.0% |
| !! | ATTTAAAATCTACTAGTGCT+AGG | - | chr4.3:2044105-2044124 | None:intergenic | 25.0% |
| !! | TTCCTGATATTTAGGATTAT+GGG | + | chr4.3:2043485-2043504 | MS.gene015741:intron | 25.0% |
| !! | TTTCCTGATATTTAGGATTA+TGG | + | chr4.3:2043484-2043503 | MS.gene015741:intron | 25.0% |
| !!! | TGATTTTTGGTTTTAGGATA+TGG | + | chr4.3:2043152-2043171 | MS.gene015741:intron | 25.0% |
| !!! | TGTTTGCTTTTTATTCACAT+TGG | + | chr4.3:2043783-2043802 | MS.gene015741:intron | 25.0% |
| AAGAATGAATGCCAAAAATC+TGG | + | chr4.3:2043728-2043747 | MS.gene015741:intron | 30.0% | |
| CAATTAAGGTGACAAATCTA+CGG | - | chr4.3:2044003-2044022 | None:intergenic | 30.0% | |
| CTTTACATCATCAATAAGTC+CGG | + | chr4.3:2042840-2042859 | MS.gene015741:CDS | 30.0% | |
| TACATACAGGTAAAATTCCA+AGG | - | chr4.3:2043918-2043937 | None:intergenic | 30.0% | |
| ! | AATAAGTTGGGTTGTTAATC+AGG | + | chr4.3:2042998-2043017 | MS.gene015741:intron | 30.0% |
| ! | AGTGATATGTACCAGATTTT+TGG | - | chr4.3:2043742-2043761 | None:intergenic | 30.0% |
| ! | CAAACATAATTGGTAACAAC+TGG | - | chr4.3:2043972-2043991 | None:intergenic | 30.0% |
| ! | CTGGGTTTTACTAACATTTA+AGG | + | chr4.3:2043849-2043868 | MS.gene015741:intron | 30.0% |
| !!! | CGTCATTGATTTTTGGTTTT+AGG | + | chr4.3:2043146-2043165 | MS.gene015741:intron | 30.0% |
| !!! | CTATTCACGTCATTGATTTT+TGG | + | chr4.3:2043139-2043158 | MS.gene015741:intron | 30.0% |
| AAATAATACCTGTCAGTGAC+TGG | - | chr4.3:2043657-2043676 | None:intergenic | 35.0% | |
| AATTAATTCTTCTCTGCCAC+AGG | + | chr4.3:2044134-2044153 | MS.gene015741:intron | 35.0% | |
| ACCCAACTTATTCATCAAAG+AGG | - | chr4.3:2042990-2043009 | None:intergenic | 35.0% | |
| ATCTGGTACATATCACTGAT+TGG | + | chr4.3:2043745-2043764 | MS.gene015741:intron | 35.0% | |
| ATTAATTCTTCTCTGCCACA+GGG | + | chr4.3:2044135-2044154 | MS.gene015741:intron | 35.0% | |
| CCCAACTTATTCATCAAAGA+GGG | - | chr4.3:2042989-2043008 | None:intergenic | 35.0% | |
| CCCTCTTTGATGAATAAGTT+GGG | + | chr4.3:2042986-2043005 | MS.gene015741:intron | 35.0% | |
| CGTAGATTTGTCACCTTAAT+TGG | + | chr4.3:2044001-2044020 | MS.gene015741:intron | 35.0% | |
| CTTATAGTAGATCAATCCAC+CGG | - | chr4.3:2042862-2042881 | None:intergenic | 35.0% | |
| CTTTGATATCAACTTAGCTC+AGG | + | chr4.3:2044292-2044311 | MS.gene015741:CDS | 35.0% | |
| GACCCATAATCCTAAATATC+AGG | - | chr4.3:2043490-2043509 | None:intergenic | 35.0% | |
| TACTAGTGCTAGGTTATAGA+TGG | - | chr4.3:2044095-2044114 | None:intergenic | 35.0% | |
| TCACGTCGTAGATGAAAATA+AGG | + | chr4.3:2043177-2043196 | MS.gene015741:intron | 35.0% | |
| TGTAATGCCTTGGTCATTTA+AGG | + | chr4.3:2043934-2043953 | MS.gene015741:intron | 35.0% | |
| TTAATCAGGTCACTTGAACT+TGG | + | chr4.3:2043012-2043031 | MS.gene015741:intron | 35.0% | |
| TTCACATTGGTAAGTAACCT+TGG | + | chr4.3:2043796-2043815 | MS.gene015741:intron | 35.0% | |
| TTTACCTGTATGTAATGCCT+TGG | + | chr4.3:2043924-2043943 | MS.gene015741:intron | 35.0% | |
| TTTGTCAAGGCTGTTAATCA+AGG | - | chr4.3:2043323-2043342 | None:intergenic | 35.0% | |
| ! | ATTTAGGATTATGGGTCTTC+TGG | + | chr4.3:2043493-2043512 | MS.gene015741:intron | 35.0% |
| !! | TAACATAGTCTTATGTGCCT+TGG | + | chr4.3:2043898-2043917 | MS.gene015741:intron | 35.0% |
| ACATTGGTAAGTAACCTTGG+AGG | + | chr4.3:2043799-2043818 | MS.gene015741:intron | 40.0% | |
| AGTAGAGACTAGAGAGCAAT+GGG | - | chr4.3:2042901-2042920 | None:intergenic | 40.0% | |
| CAGATTCTTGCAAAGGCTTT+GGG | + | chr4.3:2042798-2042817 | MS.gene015741:CDS | 40.0% | |
| CAGCCTTGACAAATTCACAA+CGG | + | chr4.3:2043330-2043349 | MS.gene015741:intron | 40.0% | |
| GAAGATCAAATGTGTCAGCT+TGG | - | chr4.3:2043627-2043646 | None:intergenic | 40.0% | |
| GCCCTCTTTGATGAATAAGT+TGG | + | chr4.3:2042985-2043004 | MS.gene015741:intron | 40.0% | |
| GGCATTTCAGATTCTTGCAA+AGG | + | chr4.3:2042791-2042810 | MS.gene015741:CDS | 40.0% | |
| TACATCATCAATAAGTCCGG+TGG | + | chr4.3:2042843-2042862 | MS.gene015741:CDS | 40.0% | |
| TAGTCTCTACTCTCTACTGT+GGG | + | chr4.3:2042910-2042929 | MS.gene015741:intron | 40.0% | |
| TCAAAGAGCTCACATCGTAT+AGG | - | chr4.3:2044278-2044297 | None:intergenic | 40.0% | |
| TCAGATTCTTGCAAAGGCTT+TGG | + | chr4.3:2042797-2042816 | MS.gene015741:CDS | 40.0% | |
| TGAACCTGGAACTCAACAAA+TGG | + | chr4.3:2044178-2044197 | MS.gene015741:CDS | 40.0% | |
| TGGACACCAATGATAGCTTA+CGG | + | chr4.3:2043521-2043540 | MS.gene015741:CDS | 40.0% | |
| ! | ATGACCAAGGCATTACATAC+AGG | - | chr4.3:2043931-2043950 | None:intergenic | 40.0% |
| ! | CTTTCCATTTGTTGAGTTCC+AGG | - | chr4.3:2044185-2044204 | None:intergenic | 40.0% |
| ! | CTTTCTGAGTTAGATGGGTT+TGG | - | chr4.3:2043379-2043398 | None:intergenic | 40.0% |
| !! | CGGTGGATTGATCTACTATA+AGG | + | chr4.3:2042860-2042879 | MS.gene015741:CDS | 40.0% |
| ACGCCGTTGTGAATTTGTCA+AGG | - | chr4.3:2043336-2043355 | None:intergenic | 45.0% | |
| AGGCATCTCCATCTCATAGA+AGG | - | chr4.3:2044258-2044277 | None:intergenic | 45.0% | |
| CTAGTCTCTACTCTCTACTG+TGG | + | chr4.3:2042909-2042928 | MS.gene015741:intron | 45.0% | |
| GAAGAACCCCTTCTATGAGA+TGG | + | chr4.3:2044247-2044266 | MS.gene015741:CDS | 45.0% | |
| GAGTAGAGACTAGAGAGCAA+TGG | - | chr4.3:2042902-2042921 | None:intergenic | 45.0% | |
| GCATCTCCATCTCATAGAAG+GGG | - | chr4.3:2044256-2044275 | None:intergenic | 45.0% | |
| GGACACCAATGATAGCTTAC+GGG | + | chr4.3:2043522-2043541 | MS.gene015741:CDS | 45.0% | |
| GGCATCTCCATCTCATAGAA+GGG | - | chr4.3:2044257-2044276 | None:intergenic | 45.0% | |
| GTGAACGCAAAGGCCAATTA+AGG | - | chr4.3:2044017-2044036 | None:intergenic | 45.0% | |
| TTATGGGTCTTCTGGACGAA+TGG | + | chr4.3:2043501-2043520 | MS.gene015741:CDS | 45.0% | |
| ! | CAAGGCACCTTAAATGACCA+AGG | - | chr4.3:2043944-2043963 | None:intergenic | 45.0% |
| ! | TTTTTCGTGGTCTGTGAACC+TGG | + | chr4.3:2044164-2044183 | MS.gene015741:CDS | 45.0% |
| AAGGACCGTGTTGCATTGCT+GGG | + | chr4.3:2044323-2044342 | MS.gene015741:CDS | 50.0% | |
| AGCCACCCGTAAGCTATCAT+TGG | - | chr4.3:2043530-2043549 | None:intergenic | 50.0% | |
| AGGTGACAACTGCTGAGAGA+TGG | - | chr4.3:2043575-2043594 | None:intergenic | 50.0% | |
| ATCGACCCAGCAATGCAACA+CGG | - | chr4.3:2044331-2044350 | None:intergenic | 50.0% | |
| ATTCCTGCGCAACCAGAAAC+AGG | - | chr4.3:2043595-2043614 | None:intergenic | 50.0% | |
| CACCAATGATAGCTTACGGG+TGG | + | chr4.3:2043525-2043544 | MS.gene015741:CDS | 50.0% | |
| CACGAAAAACTTCGTCCCTG+TGG | - | chr4.3:2044153-2044172 | None:intergenic | 50.0% | |
| CAGCAGTTGTCACCTGTTTC+TGG | + | chr4.3:2043580-2043599 | MS.gene015741:CDS | 50.0% | |
| CAGCATCACAGTGAACGCAA+AGG | - | chr4.3:2044027-2044046 | None:intergenic | 50.0% | |
| CATTGCTTCCAGTCACTGAC+AGG | + | chr4.3:2043646-2043665 | MS.gene015741:CDS | 50.0% | |
| TGGTAACAACTGGTGCAGCA+AGG | - | chr4.3:2043962-2043981 | None:intergenic | 50.0% | |
| ! | CACAGGGACGAAGTTTTTCG+TGG | + | chr4.3:2044151-2044170 | MS.gene015741:intron | 50.0% |
| CTTAGCTCAGGCAGTGCAGA+AGG | + | chr4.3:2044304-2044323 | MS.gene015741:CDS | 55.0% | |
| GAAGGACCGTGTTGCATTGC+TGG | + | chr4.3:2044322-2044341 | MS.gene015741:CDS | 55.0% | |
| GTACTGTGCAGTCTGTGCTG+TGG | + | chr4.3:2043826-2043845 | MS.gene015741:intron | 55.0% | |
| TGCACAGTACAGTGCCTCCA+AGG | - | chr4.3:2043816-2043835 | None:intergenic | 55.0% | |
| !! | GCTTACGGGTGGCTAGCTTA+TGG | + | chr4.3:2043536-2043555 | MS.gene015741:CDS | 55.0% |
| !! | TCACCTGTTTCTGGTTGCGC+AGG | + | chr4.3:2043589-2043608 | MS.gene015741:CDS | 55.0% |
| GTGCAGTCTGTGCTGTGGCT+GGG | + | chr4.3:2043831-2043850 | MS.gene015741:intron | 60.0% | |
| TGTGCAGTCTGTGCTGTGGC+TGG | + | chr4.3:2043830-2043849 | MS.gene015741:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 2042789 | 2044352 | 2042789 | ID=MS.gene015741 |
| chr4.3 | mRNA | 2042789 | 2044352 | 2042789 | ID=MS.gene015741.t1;Parent=MS.gene015741 |
| chr4.3 | exon | 2042789 | 2042881 | 2042789 | ID=MS.gene015741.t1.exon1;Parent=MS.gene015741.t1 |
| chr4.3 | CDS | 2042789 | 2042881 | 2042789 | ID=cds.MS.gene015741.t1;Parent=MS.gene015741.t1 |
| chr4.3 | exon | 2043499 | 2043667 | 2043499 | ID=MS.gene015741.t1.exon2;Parent=MS.gene015741.t1 |
| chr4.3 | CDS | 2043499 | 2043667 | 2043499 | ID=cds.MS.gene015741.t1;Parent=MS.gene015741.t1 |
| chr4.3 | exon | 2044156 | 2044352 | 2044156 | ID=MS.gene015741.t1.exon3;Parent=MS.gene015741.t1 |
| chr4.3 | CDS | 2044156 | 2044352 | 2044156 | ID=cds.MS.gene015741.t1;Parent=MS.gene015741.t1 |
| Gene Sequence |
| Protein sequence |
