Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015836.t1 | AFK40156.1 | 98.5 | 131 | 2 | 0 | 1 | 131 | 1 | 131 | 1.80E-70 | 275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015836.t1 | P49231 | 88.5 | 131 | 15 | 0 | 1 | 131 | 1 | 131 | 1.3e-66 | 253.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015836.t1 | I3SIR4 | 98.5 | 131 | 2 | 0 | 1 | 131 | 1 | 131 | 1.3e-70 | 275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015836 | MS.gene20133 | PPI |
| MS.gene015836 | MS.gene015837 | PPI |
| MS.gene015836 | MS.gene20134 | PPI |
| MS.gene015836 | MS.gene55068 | PPI |
| MS.gene20133 | MS.gene015836 | PPI |
| MS.gene015836 | MS.gene049055 | PPI |
| MS.gene015836 | MS.gene049056 | PPI |
| MS.gene015836 | MS.gene86676 | PPI |
| MS.gene015836 | MS.gene065414 | PPI |
| MS.gene015836 | MS.gene05946 | PPI |
| MS.gene049056 | MS.gene015836 | PPI |
| MS.gene015836 | MS.gene05947 | PPI |
| MS.gene015836 | MS.gene024155 | PPI |
| MS.gene015836 | MS.gene033590 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015836.t1 | MTR_8g102330 | 89.474 | 133 | 12 | 1 | 1 | 131 | 1 | 133 | 8.58e-86 | 246 |
| MS.gene015836.t1 | MTR_6g090030 | 78.947 | 133 | 26 | 1 | 1 | 131 | 1 | 133 | 9.62e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015836.t1 | AT2G19760 | 79.389 | 131 | 27 | 0 | 1 | 131 | 1 | 131 | 8.93e-76 | 221 |
| MS.gene015836.t1 | AT5G56600 | 79.389 | 131 | 27 | 0 | 1 | 131 | 1 | 131 | 9.53e-76 | 221 |
| MS.gene015836.t1 | AT4G29350 | 77.863 | 131 | 29 | 0 | 1 | 131 | 1 | 131 | 1.48e-75 | 220 |
| MS.gene015836.t1 | AT2G19770 | 76.119 | 134 | 29 | 1 | 1 | 131 | 1 | 134 | 9.55e-75 | 218 |
| MS.gene015836.t1 | AT4G29340 | 75.373 | 134 | 30 | 1 | 1 | 131 | 1 | 134 | 1.89e-72 | 213 |
| MS.gene015836.t1 | AT5G56600 | 78.161 | 87 | 19 | 0 | 1 | 87 | 1 | 87 | 3.36e-46 | 145 |
Find 39 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGATTATCTTGTTGAAC+AGG | 0.316615 | 4.3:-3164911 | MS.gene015836:CDS |
| TTGATAGCAGTTATCTCTTC+AGG | 0.316863 | 4.3:+3165913 | None:intergenic |
| TATTGGTGGTACTAAATATA+TGG | 0.344442 | 4.3:-3165845 | MS.gene015836:CDS |
| CCAGTTGGAGCAAGAGTTCC+AGG | 0.346308 | 4.3:+3165874 | None:intergenic |
| GCTCCAACTGGATTACATAT+TGG | 0.350525 | 4.3:-3165862 | MS.gene015836:CDS |
| ACCAATATGGCCTTGGTCAT+TGG | 0.371913 | 4.3:-3164988 | MS.gene015836:CDS |
| GCCAATGACCAAGGCCATAT+TGG | 0.385130 | 4.3:+3164987 | None:intergenic |
| CCTGGAACTCTTGCTCCAAC+TGG | 0.397773 | 4.3:-3165874 | MS.gene015836:CDS |
| TCGGTGTAGACGGTAGCGTT+TGG | 0.405021 | 4.3:-3166317 | MS.gene015836:CDS |
| CGGTGTAGACGGTAGCGTTT+GGG | 0.413449 | 4.3:-3166316 | MS.gene015836:CDS |
| TTGCACTGACCGGGAGTCAT+TGG | 0.432121 | 4.3:+3164952 | None:intergenic |
| AACTACCATGTTGCACTGAC+CGG | 0.438554 | 4.3:+3164942 | None:intergenic |
| TTCCCTCGAATGACAGCTCC+AGG | 0.451609 | 4.3:+3165808 | None:intergenic |
| CACCTTCTCTGTGACATTGA+AGG | 0.461970 | 4.3:-3166366 | MS.gene015836:CDS |
| AATAAAGACTTCGATGAGCC+TGG | 0.476770 | 4.3:-3165892 | MS.gene015836:CDS |
| ATTATATTATCCCAGGGTGC+TGG | 0.481662 | 4.3:-3165030 | MS.gene015836:intron |
| ACTAAATATATGGTGATTCA+AGG | 0.491883 | 4.3:-3165835 | MS.gene015836:CDS |
| TACTGTTAAGAAGACCAATA+TGG | 0.497722 | 4.3:-3165001 | MS.gene015836:CDS |
| TAAGAAGACCAATATGGCCT+TGG | 0.505382 | 4.3:-3164995 | MS.gene015836:CDS |
| CTCACTCATGCTGCTATCCT+CGG | 0.510155 | 4.3:-3166336 | MS.gene015836:CDS |
| ATGGTGATTCAAGGTGAACC+TGG | 0.519803 | 4.3:-3165826 | MS.gene015836:CDS |
| GAACCTGGAGCTGTCATTCG+AGG | 0.527391 | 4.3:-3165811 | MS.gene015836:CDS |
| AGCTGTCATTCGAGGGAAGA+AGG | 0.545445 | 4.3:-3165803 | MS.gene015836:intron |
| GCTGCTATCCTCGGTGTAGA+CGG | 0.548044 | 4.3:-3166327 | MS.gene015836:CDS |
| TCAGAGTGCTAATTTCCCTC+AGG | 0.551578 | 4.3:-3166292 | MS.gene015836:intron |
| ACTACCATGTTGCACTGACC+GGG | 0.553161 | 4.3:+3164943 | None:intergenic |
| ATATTATCCCAGGGTGCTGG+TGG | 0.563632 | 4.3:-3165027 | MS.gene015836:intron |
| CCAACTGGATTACATATTGG+TGG | 0.577382 | 4.3:-3165859 | MS.gene015836:CDS |
| GGTGATTATCTTGTTGAACA+GGG | 0.591801 | 4.3:-3164910 | MS.gene015836:CDS |
| TACCTTCAATGTCACAGAGA+AGG | 0.600366 | 4.3:+3166364 | None:intergenic |
| ATCATAAATGCCAATGACCA+AGG | 0.602159 | 4.3:+3164978 | None:intergenic |
| TATGATGAACCAATGACTCC+CGG | 0.606226 | 4.3:-3164961 | MS.gene015836:CDS |
| CCACCAATATGTAATCCAGT+TGG | 0.607406 | 4.3:+3165859 | None:intergenic |
| AACCTGGAGCTGTCATTCGA+GGG | 0.609180 | 4.3:-3165810 | MS.gene015836:CDS |
| AGTGCAACATGGTAGTTGAA+AGG | 0.610554 | 4.3:-3164936 | MS.gene015836:CDS |
| GACTCCCGGTCAGTGCAACA+TGG | 0.634244 | 4.3:-3164947 | MS.gene015836:CDS |
| AACATGGTAGTTGAAAGGCT+TGG | 0.685081 | 4.3:-3164931 | MS.gene015836:CDS |
| AATCTCTGTGAAGCATGTCG+TGG | 0.689357 | 4.3:-3166407 | None:intergenic |
| AAACGCTACCGTCTACACCG+AGG | 0.799540 | 4.3:+3166319 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTCTGAATACTTATTTT+GGG | - | chr4.3:3166242-3166261 | MS.gene015836:intron | 15.0% |
| !!! | TAATTTCTGAATACTTATTT+TGG | - | chr4.3:3166241-3166260 | MS.gene015836:intron | 15.0% |
| !!! | TATTTTAATCTTTCAAATTG+AGG | - | chr4.3:3166030-3166049 | MS.gene015836:intron | 15.0% |
| !!! | TTTTAATTTGATAGAGAAAA+TGG | + | chr4.3:3165929-3165948 | None:intergenic | 15.0% |
| !! | ATTGAAAATCTATTTGAAAG+TGG | - | chr4.3:3165948-3165967 | MS.gene015836:intron | 20.0% |
| !! | TTGAAAATCTATTTGAAAGT+GGG | - | chr4.3:3165949-3165968 | MS.gene015836:intron | 20.0% |
| !!! | AACAGTTAAATGCTATTTAT+TGG | - | chr4.3:3166094-3166113 | MS.gene015836:intron | 20.0% |
| !!! | AATGCCATTTTACTATTTTA+GGG | - | chr4.3:3165820-3165839 | MS.gene015836:CDS | 20.0% |
| !!! | TGGTCAATTTTTTTATTCAA+TGG | - | chr4.3:3166167-3166186 | MS.gene015836:intron | 20.0% |
| ! | AAACTGCTCTATTTAATTGT+AGG | - | chr4.3:3165620-3165639 | MS.gene015836:intron | 25.0% |
| ! | AATAGCATTTAACTGTTCAA+AGG | + | chr4.3:3166091-3166110 | None:intergenic | 25.0% |
| ! | AATTTCAAAAAGACCAAACA+TGG | + | chr4.3:3165652-3165671 | None:intergenic | 25.0% |
| ! | ACATCCCTAAAATAGTAAAA+TGG | + | chr4.3:3165827-3165846 | None:intergenic | 25.0% |
| ! | ACTAAATATATGGTGATTCA+AGG | - | chr4.3:3165461-3165480 | MS.gene015836:intron | 25.0% |
| ! | ATTGATCTGAATAAGTCTTA+TGG | - | chr4.3:3166147-3166166 | MS.gene015836:intron | 25.0% |
| ! | CTTATTCAGATCAATATTGA+AGG | + | chr4.3:3166142-3166161 | None:intergenic | 25.0% |
| ! | GAGATTCAATCTAACATAAT+AGG | + | chr4.3:3165599-3165618 | None:intergenic | 25.0% |
| ! | TACAATTTCAGAAATCACAA+TGG | - | chr4.3:3165890-3165909 | MS.gene015836:CDS | 25.0% |
| ! | TTATACATTGTGAAGTGTAA+TGG | - | chr4.3:3165282-3165301 | MS.gene015836:intron | 25.0% |
| !! | GTTCTTTATTTGTAATGTGT+TGG | - | chr4.3:3165335-3165354 | MS.gene015836:intron | 25.0% |
| !! | TAACCTTACACTGATTATTA+AGG | - | chr4.3:3165144-3165163 | MS.gene015836:intron | 25.0% |
| !! | TATTGGTGGTACTAAATATA+TGG | - | chr4.3:3165451-3165470 | MS.gene015836:intron | 25.0% |
| !!! | GAATGCCATTTTACTATTTT+AGG | - | chr4.3:3165819-3165838 | MS.gene015836:CDS | 25.0% |
| !!! | GTTTGGTCTTTTTGAAATTT+AGG | - | chr4.3:3165653-3165672 | MS.gene015836:intron | 25.0% |
| !!! | TTGAAGGTAATTTTTTATGC+AGG | + | chr4.3:3166126-3166145 | None:intergenic | 25.0% |
| AGTCAAATTCTCTGATGAAT+CGG | + | chr4.3:3165204-3165223 | None:intergenic | 30.0% | |
| ATATAAGCAAGTTATTGTGC+AGG | + | chr4.3:3166222-3166241 | None:intergenic | 30.0% | |
| CAAATGAAGTTCTGTACTTA+GGG | - | chr4.3:3165176-3165195 | MS.gene015836:intron | 30.0% | |
| GATCCTTAATAATCAGTGTA+AGG | + | chr4.3:3165150-3165169 | None:intergenic | 30.0% | |
| GTTGTCAATTTAGGAATTTC+AGG | - | chr4.3:3165848-3165867 | MS.gene015836:CDS | 30.0% | |
| TACTGTTAAGAAGACCAATA+TGG | - | chr4.3:3166295-3166314 | MS.gene015836:CDS | 30.0% | |
| !! | CATTTTAGTCACACATTTCA+TGG | + | chr4.3:3165800-3165819 | None:intergenic | 30.0% |
| AGGGATGTTGTTGTCAATTT+AGG | - | chr4.3:3165839-3165858 | MS.gene015836:CDS | 35.0% | |
| ATCATAAATGCCAATGACCA+AGG | + | chr4.3:3166321-3166340 | None:intergenic | 35.0% | |
| ATCTATGCTATGCTATGCTT+TGG | - | chr4.3:3165563-3165582 | MS.gene015836:intron | 35.0% | |
| GCAAATGAAGTTCTGTACTT+AGG | - | chr4.3:3165175-3165194 | MS.gene015836:intron | 35.0% | |
| GGTGATTATCTTGTTGAACA+GGG | - | chr4.3:3166386-3166405 | MS.gene015836:CDS | 35.0% | |
| TGGTGATTATCTTGTTGAAC+AGG | - | chr4.3:3166385-3166404 | MS.gene015836:CDS | 35.0% | |
| TTGGGCATTATATTATCCCA+GGG | - | chr4.3:3166260-3166279 | MS.gene015836:intron | 35.0% | |
| TTTGGGCATTATATTATCCC+AGG | - | chr4.3:3166259-3166278 | MS.gene015836:intron | 35.0% | |
| ! | TTGATAGCAGTTATCTCTTC+AGG | + | chr4.3:3165386-3165405 | None:intergenic | 35.0% |
| AACATGGTAGTTGAAAGGCT+TGG | - | chr4.3:3166365-3166384 | MS.gene015836:CDS | 40.0% | |
| AATAAAGACTTCGATGAGCC+TGG | - | chr4.3:3165404-3165423 | MS.gene015836:intron | 40.0% | |
| AGTGCAACATGGTAGTTGAA+AGG | - | chr4.3:3166360-3166379 | MS.gene015836:CDS | 40.0% | |
| ATTATATTATCCCAGGGTGC+TGG | - | chr4.3:3166266-3166285 | MS.gene015836:intron | 40.0% | |
| CCAACTGGATTACATATTGG+TGG | - | chr4.3:3165437-3165456 | MS.gene015836:intron | 40.0% | |
| CCAATCACAGTAACAGAGAT+CGG | + | chr4.3:3165120-3165139 | None:intergenic | 40.0% | |
| CCACCAATATGTAATCCAGT+TGG | + | chr4.3:3165440-3165459 | None:intergenic | 40.0% | |
| GCTCCAACTGGATTACATAT+TGG | - | chr4.3:3165434-3165453 | MS.gene015836:intron | 40.0% | |
| TAAGAAGACCAATATGGCCT+TGG | - | chr4.3:3166301-3166320 | MS.gene015836:CDS | 40.0% | |
| TACCTTCAATGTCACAGAGA+AGG | + | chr4.3:3164935-3164954 | None:intergenic | 40.0% | |
| TATGATGAACCAATGACTCC+CGG | - | chr4.3:3166335-3166354 | MS.gene015836:CDS | 40.0% | |
| TTGTAGGTTGTTGCCATGTT+TGG | - | chr4.3:3165636-3165655 | MS.gene015836:intron | 40.0% | |
| ! | CACAAGCATTGCCAATGATA+AGG | + | chr4.3:3165981-3166000 | None:intergenic | 40.0% |
| !! | CAGATCGATGGATTGATGAA+AGG | + | chr4.3:3165032-3165051 | None:intergenic | 40.0% |
| !! | GATTGATGAAAGGTACCTGA+GGG | + | chr4.3:3165022-3165041 | None:intergenic | 40.0% |
| ATGGTGATTCAAGGTGAACC+TGG | - | chr4.3:3165470-3165489 | MS.gene015836:intron | 45.0% | |
| CACCTTCTCTGTGACATTGA+AGG | - | chr4.3:3164930-3164949 | MS.gene015836:CDS | 45.0% | |
| CCGATCTCTGTTACTGTGAT+TGG | - | chr4.3:3165117-3165136 | MS.gene015836:intron | 45.0% | |
| TCGCGAAAGAAACAGATCGA+TGG | + | chr4.3:3165044-3165063 | None:intergenic | 45.0% | |
| ! | AACTACCATGTTGCACTGAC+CGG | + | chr4.3:3166357-3166376 | None:intergenic | 45.0% |
| ! | ACCAATATGGCCTTGGTCAT+TGG | - | chr4.3:3166308-3166327 | MS.gene015836:CDS | 45.0% |
| ! | GTGGGTTGCAACCTTATCAT+TGG | - | chr4.3:3165967-3165986 | MS.gene015836:intron | 45.0% |
| ! | TCAGAGTGCTAATTTCCCTC+AGG | - | chr4.3:3165004-3165023 | MS.gene015836:CDS | 45.0% |
| !! | GGATTGATGAAAGGTACCTG+AGG | + | chr4.3:3165023-3165042 | None:intergenic | 45.0% |
| !! | TAATTAATTAATTAATTAAG+TGG | - | chr4.3:3165242-3165261 | MS.gene015836:intron | 5.0% |
| AACCTGGAGCTGTCATTCGA+GGG | - | chr4.3:3165486-3165505 | MS.gene015836:intron | 50.0% | |
| AGCTGTCATTCGAGGGAAGA+AGG | - | chr4.3:3165493-3165512 | MS.gene015836:intron | 50.0% | |
| CTCACTCATGCTGCTATCCT+CGG | - | chr4.3:3164960-3164979 | MS.gene015836:CDS | 50.0% | |
| GCCAATGACCAAGGCCATAT+TGG | + | chr4.3:3166312-3166331 | None:intergenic | 50.0% | |
| ! | ACTACCATGTTGCACTGACC+GGG | + | chr4.3:3166356-3166375 | None:intergenic | 50.0% |
| !! | ATATTATCCCAGGGTGCTGG+TGG | - | chr4.3:3166269-3166288 | MS.gene015836:intron | 50.0% |
| AAACGCTACCGTCTACACCG+AGG | + | chr4.3:3164980-3164999 | None:intergenic | 55.0% | |
| AGTAACACCACCAGCACCCT+GGG | + | chr4.3:3166279-3166298 | None:intergenic | 55.0% | |
| CCAGTTGGAGCAAGAGTTCC+AGG | + | chr4.3:3165425-3165444 | None:intergenic | 55.0% | |
| CCTGGAACTCTTGCTCCAAC+TGG | - | chr4.3:3165422-3165441 | MS.gene015836:intron | 55.0% | |
| CGGTGTAGACGGTAGCGTTT+GGG | - | chr4.3:3164980-3164999 | MS.gene015836:CDS | 55.0% | |
| GAACCTGGAGCTGTCATTCG+AGG | - | chr4.3:3165485-3165504 | MS.gene015836:intron | 55.0% | |
| TCGGTGTAGACGGTAGCGTT+TGG | - | chr4.3:3164979-3164998 | MS.gene015836:CDS | 55.0% | |
| TTCCCTCGAATGACAGCTCC+AGG | + | chr4.3:3165491-3165510 | None:intergenic | 55.0% | |
| ! | GCTGCTATCCTCGGTGTAGA+CGG | - | chr4.3:3164969-3164988 | MS.gene015836:CDS | 55.0% |
| ! | TTGCACTGACCGGGAGTCAT+TGG | + | chr4.3:3166347-3166366 | None:intergenic | 55.0% |
| CAGTAACACCACCAGCACCC+TGG | + | chr4.3:3166280-3166299 | None:intergenic | 60.0% | |
| GACTCCCGGTCAGTGCAACA+TGG | - | chr4.3:3166349-3166368 | MS.gene015836:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 3164903 | 3166415 | 3164903 | ID=MS.gene015836 |
| chr4.3 | mRNA | 3164903 | 3166415 | 3164903 | ID=MS.gene015836.t1;Parent=MS.gene015836 |
| chr4.3 | exon | 3166293 | 3166415 | 3166293 | ID=MS.gene015836.t1.exon1;Parent=MS.gene015836.t1 |
| chr4.3 | CDS | 3166293 | 3166415 | 3166293 | ID=cds.MS.gene015836.t1;Parent=MS.gene015836.t1 |
| chr4.3 | exon | 3165804 | 3165941 | 3165804 | ID=MS.gene015836.t1.exon2;Parent=MS.gene015836.t1 |
| chr4.3 | CDS | 3165804 | 3165941 | 3165804 | ID=cds.MS.gene015836.t1;Parent=MS.gene015836.t1 |
| chr4.3 | exon | 3164903 | 3165037 | 3164903 | ID=MS.gene015836.t1.exon3;Parent=MS.gene015836.t1 |
| chr4.3 | CDS | 3164903 | 3165037 | 3164903 | ID=cds.MS.gene015836.t1;Parent=MS.gene015836.t1 |
| Gene Sequence |
| Protein sequence |
