Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05947.t1 | XP_024628281.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.60E-71 | 278.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05947.t1 | A4K9Z8 | 90.2 | 133 | 13 | 0 | 1 | 133 | 1 | 133 | 8.5e-69 | 260.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05947.t1 | A0A396GR17 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.2e-71 | 278.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05947 | MS.gene20134 | 0.946743 | 2.57E-105 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene030883 | MS.gene05947 | PPI |
| MS.gene79962 | MS.gene05947 | PPI |
| MS.gene05947 | MS.gene015837 | PPI |
| MS.gene05947 | MS.gene20134 | PPI |
| MS.gene044849 | MS.gene05947 | PPI |
| MS.gene001874 | MS.gene05947 | PPI |
| MS.gene015836 | MS.gene05947 | PPI |
| MS.gene20133 | MS.gene05947 | PPI |
| MS.gene05947 | MS.gene049055 | PPI |
| MS.gene27990 | MS.gene05947 | PPI |
| MS.gene05947 | MS.gene05946 | PPI |
| MS.gene049056 | MS.gene05947 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05947.t1 | MTR_6g090030 | 89.474 | 133 | 14 | 0 | 1 | 133 | 1 | 133 | 6.18e-88 | 252 |
| MS.gene05947.t1 | MTR_8g102330 | 84.211 | 133 | 21 | 0 | 1 | 133 | 1 | 133 | 1.91e-83 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05947.t1 | AT2G19770 | 86.567 | 134 | 17 | 1 | 1 | 133 | 1 | 134 | 1.37e-85 | 246 |
| MS.gene05947.t1 | AT4G29340 | 84.328 | 134 | 20 | 1 | 1 | 133 | 1 | 134 | 2.46e-83 | 240 |
| MS.gene05947.t1 | AT2G19760 | 75.188 | 133 | 31 | 1 | 1 | 133 | 1 | 131 | 2.03e-71 | 210 |
| MS.gene05947.t1 | AT4G29350 | 73.684 | 133 | 33 | 1 | 1 | 133 | 1 | 131 | 1.18e-70 | 208 |
| MS.gene05947.t1 | AT5G56600 | 72.180 | 133 | 35 | 1 | 1 | 133 | 1 | 131 | 6.70e-69 | 204 |
| MS.gene05947.t1 | AT5G56600 | 70.787 | 89 | 24 | 1 | 1 | 89 | 1 | 87 | 2.99e-41 | 133 |
Find 0 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAACATTGTAATTAAAA+TGG | + | 56947:53503-53522 | None:intergenic | 15.0% |
| !! | TTTGAAGATTAGATTTAATT+AGG | - | 56947:53642-53661 | MS.gene05947:intron | 15.0% |
| !!! | ATTTTGAAAGTAATTATACT+TGG | - | 56947:53371-53390 | MS.gene05947:intron | 15.0% |
| !! | ATCATCATTGTAAAGATTTA+CGG | + | 56947:53436-53455 | None:intergenic | 20.0% |
| ! | GAAGATTAGATTTAATTAGG+AGG | - | 56947:53645-53664 | MS.gene05947:intron | 25.0% |
| ! | TGTAATCATGAATATACTTG+AGG | + | 56947:53203-53222 | None:intergenic | 25.0% |
| !! | TATGTAAGATCGATTTAGAA+AGG | + | 56947:53581-53600 | None:intergenic | 25.0% |
| !!! | TGATATATGTGCTTGTTATT+TGG | - | 56947:54106-54125 | MS.gene05947:intron | 25.0% |
| AAAAAAGAGACATGTACGTT+AGG | + | 56947:54067-54086 | None:intergenic | 30.0% | |
| AATAATTTACCTTCTTCCCA+CGG | + | 56947:53864-53883 | None:intergenic | 30.0% | |
| ATAATATCAGTGATCTCTTG+AGG | + | 56947:53745-53764 | None:intergenic | 30.0% | |
| ATGCATAGAATAGTTTCATG+TGG | + | 56947:53229-53248 | None:intergenic | 30.0% | |
| CATTTGATGTGTGATATTGA+TGG | - | 56947:53104-53123 | MS.gene05947:CDS | 30.0% | |
| TCATGAATATACTTGAGGAA+AGG | + | 56947:53198-53217 | None:intergenic | 30.0% | |
| TGCATAGAATAGTTTCATGT+GGG | + | 56947:53228-53247 | None:intergenic | 30.0% | |
| TTCAAAATCACACTATGATC+TGG | + | 56947:53359-53378 | None:intergenic | 30.0% | |
| !! | ATGTGCTTGTTATTTGGATA+GGG | - | 56947:54112-54131 | MS.gene05947:intron | 30.0% |
| !! | TATGTGCTTGTTATTTGGAT+AGG | - | 56947:54111-54130 | MS.gene05947:intron | 30.0% |
| AAAAGTTAGTCCATAAGACG+AGG | + | 56947:53993-54012 | None:intergenic | 35.0% | |
| AAAGTTAGTCCATAAGACGA+GGG | + | 56947:53992-54011 | None:intergenic | 35.0% | |
| GCATAGATCTCTATCATTCT+TGG | - | 56947:53245-53264 | MS.gene05947:intron | 35.0% | |
| GTTGTATTTGAGTCTCATGT+TGG | - | 56947:53923-53942 | MS.gene05947:intron | 35.0% | |
| !! | ATGAAAGATTTTGACGAACC+TGG | - | 56947:53763-53782 | MS.gene05947:intron | 35.0% |
| !! | TACGTTAGGTTTTATGCATG+TGG | + | 56947:54053-54072 | None:intergenic | 35.0% |
| !! | TTGTTATTTGGATAGGGATC+TGG | - | 56947:54118-54137 | MS.gene05947:intron | 35.0% |
| !!! | TCTAGTTTTCGGTGTTTATG+AGG | - | 56947:54171-54190 | MS.gene05947:CDS | 35.0% |
| AACATGGTTGTTGAGAGGTT+GGG | - | 56947:54217-54236 | MS.gene05947:CDS | 40.0% | |
| AATGCAACATGGTTGTTGAG+AGG | - | 56947:54212-54231 | MS.gene05947:CDS | 40.0% | |
| GGAGATTACCTCATTGATCA+GGG | - | 56947:54238-54257 | MS.gene05947:CDS | 40.0% | |
| GGCATCACCATAAAGAAAAC+CGG | - | 56947:54142-54161 | MS.gene05947:CDS | 40.0% | |
| GTCAAATACATGGTCATCCA+AGG | - | 56947:53820-53839 | MS.gene05947:intron | 40.0% | |
| TTATTTGGATAGGGATCTGG+AGG | - | 56947:54121-54140 | MS.gene05947:intron | 40.0% | |
| ! | ATGTGTGATATTGATGGCAC+CGG | - | 56947:53110-53129 | MS.gene05947:CDS | 40.0% |
| !! | AGCTTGACCGGTTTTCTTTA+TGG | + | 56947:54152-54171 | None:intergenic | 40.0% |
| ACAACCATGTTGCATTGTCC+AGG | + | 56947:54208-54227 | None:intergenic | 45.0% | |
| ACCGAAAACTAGAGCTTGAC+CGG | + | 56947:54164-54183 | None:intergenic | 45.0% | |
| CAACATGGTTGTTGAGAGGT+TGG | - | 56947:54216-54235 | MS.gene05947:CDS | 45.0% | |
| CCATGTATTTGACTCCTGCA+AGG | + | 56947:53813-53832 | None:intergenic | 45.0% | |
| CCTTGCAGGAGTCAAATACA+TGG | - | 56947:53810-53829 | MS.gene05947:intron | 45.0% | |
| GGGAGATTACCTCATTGATC+AGG | - | 56947:54237-54256 | MS.gene05947:CDS | 45.0% | |
| GTTTGAGTACCCTCGTCTTA+TGG | - | 56947:53980-53999 | MS.gene05947:intron | 45.0% | |
| !!! | ACCGGTCAAGCTCTAGTTTT+CGG | - | 56947:54160-54179 | MS.gene05947:CDS | 45.0% |
| ACCTGGTCATCTTGCTCCTA+CGG | - | 56947:53780-53799 | MS.gene05947:intron | 50.0% | |
| AGAGCCATCATGGCCAATGA+TGG | + | 56947:53156-53175 | None:intergenic | 50.0% | |
| ATCATGGCCAATGATGGCAG+CGG | + | 56947:53150-53169 | None:intergenic | 50.0% | |
| ATGGTCATCCAAGGAGAGTC+TGG | - | 56947:53829-53848 | MS.gene05947:intron | 50.0% | |
| GACTCCTGGACAATGCAACA+TGG | - | 56947:54201-54220 | MS.gene05947:CDS | 50.0% | |
| TATGAGGAACCTGTGACTCC+TGG | - | 56947:54187-54206 | MS.gene05947:CDS | 50.0% | |
| TTGCATTGTCCAGGAGTCAC+AGG | + | 56947:54199-54218 | None:intergenic | 50.0% | |
| ! | TGGCCATGATGGCTCTGTTT+GGG | - | 56947:53160-53179 | MS.gene05947:CDS | 50.0% |
| ! | TTGGCCATGATGGCTCTGTT+TGG | - | 56947:53159-53178 | MS.gene05947:CDS | 50.0% |
| AGCTGTCATCCGTGGGAAGA+AGG | - | 56947:53852-53871 | MS.gene05947:intron | 55.0% | |
| CAATGATGGCAGCGGCAGAT+AGG | + | 56947:53142-53161 | None:intergenic | 55.0% | |
| CCTGGTCATCTTGCTCCTAC+GGG | - | 56947:53781-53800 | MS.gene05947:intron | 55.0% | |
| CTATCTGCCGCTGCCATCAT+TGG | - | 56947:53140-53159 | MS.gene05947:CDS | 55.0% | |
| GAGCCCAAACAGAGCCATCA+TGG | + | 56947:53166-53185 | None:intergenic | 55.0% | |
| GCTGCCATCATTGGCCATGA+TGG | - | 56947:53149-53168 | MS.gene05947:CDS | 55.0% | |
| TGACAGCTCCAGACTCTCCT+TGG | + | 56947:53840-53859 | None:intergenic | 55.0% | |
| ! | AGTCTGGAGCTGTCATCCGT+GGG | - | 56947:53845-53864 | MS.gene05947:intron | 55.0% |
| AGCGGCAGATAGGTGGTGTC+CGG | + | 56947:53132-53151 | None:intergenic | 60.0% | |
| CCCGTAGGAGCAAGATGACC+AGG | + | 56947:53784-53803 | None:intergenic | 60.0% | |
| TGATGGCAGCGGCAGATAGG+TGG | + | 56947:53139-53158 | None:intergenic | 60.0% | |
| ! | GAGTCTGGAGCTGTCATCCG+TGG | - | 56947:53844-53863 | MS.gene05947:intron | 60.0% |
| CCTACGGGCTTGCACCTTGC+AGG | - | 56947:53796-53815 | MS.gene05947:intron | 65.0% | |
| CCTGCAAGGTGCAAGCCCGT+AGG | + | 56947:53799-53818 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 56947 | gene | 53077 | 54267 | 53077 | ID=MS.gene05947 |
| 56947 | mRNA | 53077 | 54267 | 53077 | ID=MS.gene05947.t1;Parent=MS.gene05947 |
| 56947 | exon | 54139 | 54267 | 54139 | ID=MS.gene05947.t1.exon1;Parent=MS.gene05947.t1 |
| 56947 | CDS | 54139 | 54267 | 54139 | ID=cds.MS.gene05947.t1;Parent=MS.gene05947.t1 |
| 56947 | exon | 53471 | 53608 | 53471 | ID=MS.gene05947.t1.exon2;Parent=MS.gene05947.t1 |
| 56947 | CDS | 53471 | 53608 | 53471 | ID=cds.MS.gene05947.t1;Parent=MS.gene05947.t1 |
| 56947 | exon | 53077 | 53211 | 53077 | ID=MS.gene05947.t1.exon3;Parent=MS.gene05947.t1 |
| 56947 | CDS | 53077 | 53211 | 53077 | ID=cds.MS.gene05947.t1;Parent=MS.gene05947.t1 |
| Gene Sequence |
| Protein sequence |
