Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015837.t1 | XP_024628281.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.60E-71 | 278.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015837.t1 | A4K9Z8 | 90.2 | 133 | 13 | 0 | 1 | 133 | 1 | 133 | 8.5e-69 | 260.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015837.t1 | A0A396GR17 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.2e-71 | 278.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene049056 | MS.gene015837 | PPI |
| MS.gene001874 | MS.gene015837 | PPI |
| MS.gene015836 | MS.gene015837 | PPI |
| MS.gene015837 | MS.gene20134 | PPI |
| MS.gene05947 | MS.gene015837 | PPI |
| MS.gene015837 | MS.gene049055 | PPI |
| MS.gene79962 | MS.gene015837 | PPI |
| MS.gene015837 | MS.gene05946 | PPI |
| MS.gene044849 | MS.gene015837 | PPI |
| MS.gene030883 | MS.gene015837 | PPI |
| MS.gene20133 | MS.gene015837 | PPI |
| MS.gene27990 | MS.gene015837 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015837.t1 | MTR_6g090030 | 89.474 | 133 | 14 | 0 | 1 | 133 | 1 | 133 | 6.18e-88 | 252 |
| MS.gene015837.t1 | MTR_8g102330 | 84.211 | 133 | 21 | 0 | 1 | 133 | 1 | 133 | 1.91e-83 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015837.t1 | AT2G19770 | 86.567 | 134 | 17 | 1 | 1 | 133 | 1 | 134 | 1.37e-85 | 246 |
| MS.gene015837.t1 | AT4G29340 | 84.328 | 134 | 20 | 1 | 1 | 133 | 1 | 134 | 2.46e-83 | 240 |
| MS.gene015837.t1 | AT2G19760 | 75.188 | 133 | 31 | 1 | 1 | 133 | 1 | 131 | 2.03e-71 | 210 |
| MS.gene015837.t1 | AT4G29350 | 73.684 | 133 | 33 | 1 | 1 | 133 | 1 | 131 | 1.18e-70 | 208 |
| MS.gene015837.t1 | AT5G56600 | 72.180 | 133 | 35 | 1 | 1 | 133 | 1 | 131 | 6.70e-69 | 204 |
| MS.gene015837.t1 | AT5G56600 | 70.787 | 89 | 24 | 1 | 1 | 89 | 1 | 87 | 2.99e-41 | 133 |
Find 42 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGCCATGATGGCTCTGTT+TGG | 0.298573 | 4.3:-3170100 | MS.gene015837:CDS |
| CCTACGGGCTTGCACCTTGC+AGG | 0.315777 | 4.3:-3169463 | MS.gene015837:CDS |
| TGGCCATGATGGCTCTGTTT+GGG | 0.344130 | 4.3:-3170099 | MS.gene015837:CDS |
| CTATCTGCCGCTGCCATCAT+TGG | 0.366810 | 4.3:-3170119 | MS.gene015837:CDS |
| ATGGTCATCCAAGGAGAGTC+TGG | 0.390726 | 4.3:-3169430 | MS.gene015837:CDS |
| GGGAGATTACCTCATTGATC+AGG | 0.391286 | 4.3:-3169022 | MS.gene015837:CDS |
| TTGTTATTTGGATAGGGATC+TGG | 0.425299 | 4.3:-3169141 | MS.gene015837:intron |
| CAATGATGGCAGCGGCAGAT+AGG | 0.436163 | 4.3:+3170120 | None:intergenic |
| ACCTGGTCATCTTGCTCCTA+CGG | 0.438799 | 4.3:-3169479 | MS.gene015837:CDS |
| TGACAGCTCCAGACTCTCCT+TGG | 0.476205 | 4.3:+3169422 | None:intergenic |
| AGCGGCAGATAGGTGGTGTC+CGG | 0.488939 | 4.3:+3170130 | None:intergenic |
| CCTGGTCATCTTGCTCCTAC+GGG | 0.490248 | 4.3:-3169478 | MS.gene015837:CDS |
| GCTGCCATCATTGGCCATGA+TGG | 0.491650 | 4.3:-3170110 | MS.gene015837:CDS |
| GGCATCACCATAAAGAAAAC+CGG | 0.492694 | 4.3:-3169117 | MS.gene015837:CDS |
| CATTTGATGTGTGATATTGA+TGG | 0.492739 | 4.3:-3170155 | MS.gene015837:CDS |
| TTGCATTGTCCAGGAGTCAC+AGG | 0.497282 | 4.3:+3169063 | None:intergenic |
| ACCGAAAACTAGAGCTTGAC+CGG | 0.513054 | 4.3:+3169098 | None:intergenic |
| CAACATGGTTGTTGAGAGGT+TGG | 0.513380 | 4.3:-3169043 | MS.gene015837:CDS |
| ACAACCATGTTGCATTGTCC+AGG | 0.519180 | 4.3:+3169054 | None:intergenic |
| AGCTGTCATCCGTGGGAAGA+AGG | 0.522760 | 4.3:-3169407 | MS.gene015837:intron |
| TATGAGGAACCTGTGACTCC+TGG | 0.523087 | 4.3:-3169072 | MS.gene015837:CDS |
| GAGCCCAAACAGAGCCATCA+TGG | 0.526888 | 4.3:+3170096 | None:intergenic |
| CCCGTAGGAGCAAGATGACC+AGG | 0.528322 | 4.3:+3169478 | None:intergenic |
| ATAATATCAGTGATCTCTTG+AGG | 0.540089 | 4.3:+3169517 | None:intergenic |
| AGAGCCATCATGGCCAATGA+TGG | 0.541950 | 4.3:+3170106 | None:intergenic |
| AACATGGTTGTTGAGAGGTT+GGG | 0.543359 | 4.3:-3169042 | MS.gene015837:CDS |
| GAGTCTGGAGCTGTCATCCG+TGG | 0.545615 | 4.3:-3169415 | MS.gene015837:CDS |
| GTCAAATACATGGTCATCCA+AGG | 0.546015 | 4.3:-3169439 | MS.gene015837:CDS |
| TTATTTGGATAGGGATCTGG+AGG | 0.562519 | 4.3:-3169138 | MS.gene015837:intron |
| AATAATTTACCTTCTTCCCA+CGG | 0.573845 | 4.3:+3169398 | None:intergenic |
| GGAGATTACCTCATTGATCA+GGG | 0.584642 | 4.3:-3169021 | MS.gene015837:CDS |
| CCTGCAAGGTGCAAGCCCGT+AGG | 0.594563 | 4.3:+3169463 | None:intergenic |
| ATGTGTGATATTGATGGCAC+CGG | 0.603576 | 4.3:-3170149 | MS.gene015837:CDS |
| CCTTGCAGGAGTCAAATACA+TGG | 0.605937 | 4.3:-3169449 | MS.gene015837:CDS |
| ATCATGGCCAATGATGGCAG+CGG | 0.611873 | 4.3:+3170112 | None:intergenic |
| TCATGAATATACTTGAGGAA+AGG | 0.612766 | 4.3:+3170064 | None:intergenic |
| TGATGGCAGCGGCAGATAGG+TGG | 0.614292 | 4.3:+3170123 | None:intergenic |
| GACTCCTGGACAATGCAACA+TGG | 0.634894 | 4.3:-3169058 | MS.gene015837:CDS |
| AGTCTGGAGCTGTCATCCGT+GGG | 0.647697 | 4.3:-3169414 | MS.gene015837:CDS |
| AATGCAACATGGTTGTTGAG+AGG | 0.662368 | 4.3:-3169047 | MS.gene015837:CDS |
| TCTACAGGCCCTGATCAATG+AGG | 0.683059 | 4.3:+3169013 | None:intergenic |
| CCATGTATTTGACTCCTGCA+AGG | 0.700396 | 4.3:+3169449 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAACATTGTAATTAAAA+TGG | + | chr4.3:3169440-3169459 | None:intergenic | 15.0% |
| !! | ATCATCATTGTAAAGATTTA+CGG | + | chr4.3:3169373-3169392 | None:intergenic | 20.0% |
| !! | TTTGAAGATTAGATTTGATT+AGG | - | chr4.3:3169579-3169598 | MS.gene015837:intron | 20.0% |
| ! | TGTAATCATGAATATACTTG+AGG | + | chr4.3:3169140-3169159 | None:intergenic | 25.0% |
| !! | TATGTAAGATCGATTTAGAA+AGG | + | chr4.3:3169518-3169537 | None:intergenic | 25.0% |
| !!! | TGATATATGTGCTTGTTATT+TGG | - | chr4.3:3170043-3170062 | MS.gene015837:intron | 25.0% |
| AAAAAAGAGACATGTACGTT+AGG | + | chr4.3:3170004-3170023 | None:intergenic | 30.0% | |
| AATAATTTACCTTCTTCCCA+CGG | + | chr4.3:3169801-3169820 | None:intergenic | 30.0% | |
| ATAATATCAGTGATCTCTTG+AGG | + | chr4.3:3169682-3169701 | None:intergenic | 30.0% | |
| ATGCATAGAATAGTTTCATG+TGG | + | chr4.3:3169166-3169185 | None:intergenic | 30.0% | |
| CATTTGATGTGTGATATTGA+TGG | - | chr4.3:3169041-3169060 | MS.gene015837:CDS | 30.0% | |
| GAAGATTAGATTTGATTAGG+AGG | - | chr4.3:3169582-3169601 | MS.gene015837:intron | 30.0% | |
| TCATGAATATACTTGAGGAA+AGG | + | chr4.3:3169135-3169154 | None:intergenic | 30.0% | |
| TGCATAGAATAGTTTCATGT+GGG | + | chr4.3:3169165-3169184 | None:intergenic | 30.0% | |
| TTCAAAATCACACTATGATC+TGG | + | chr4.3:3169296-3169315 | None:intergenic | 30.0% | |
| !! | ACATGCATTTTTCAAAAGAC+AGG | + | chr4.3:3169405-3169424 | None:intergenic | 30.0% |
| !! | ATGTGCTTGTTATTTGGATA+GGG | - | chr4.3:3170049-3170068 | MS.gene015837:intron | 30.0% |
| !! | TATGTGCTTGTTATTTGGAT+AGG | - | chr4.3:3170048-3170067 | MS.gene015837:intron | 30.0% |
| AAAAGTTAGTCCATAAGACG+AGG | + | chr4.3:3169930-3169949 | None:intergenic | 35.0% | |
| AAAGTTAGTCCATAAGACGA+GGG | + | chr4.3:3169929-3169948 | None:intergenic | 35.0% | |
| GCATAGATCTCTATCATTCT+TGG | - | chr4.3:3169182-3169201 | MS.gene015837:intron | 35.0% | |
| GTTGTATTTGAGTCTCATGT+TGG | - | chr4.3:3169860-3169879 | MS.gene015837:intron | 35.0% | |
| !! | ATGAAAGATTTTGACGAACC+TGG | - | chr4.3:3169700-3169719 | MS.gene015837:intron | 35.0% |
| !! | TACGTTAGGTTTTATGCATG+TGG | + | chr4.3:3169990-3170009 | None:intergenic | 35.0% |
| !! | TTGTTATTTGGATAGGGATC+TGG | - | chr4.3:3170055-3170074 | MS.gene015837:intron | 35.0% |
| !!! | TCTAGTTTTCGGTGTTTATG+AGG | - | chr4.3:3170108-3170127 | MS.gene015837:CDS | 35.0% |
| AACATGGTTGTTGAGAGGTT+GGG | - | chr4.3:3170154-3170173 | MS.gene015837:CDS | 40.0% | |
| AATGCAACATGGTTGTTGAG+AGG | - | chr4.3:3170149-3170168 | MS.gene015837:CDS | 40.0% | |
| GGAGATTACCTCATTGATCA+GGG | - | chr4.3:3170175-3170194 | MS.gene015837:CDS | 40.0% | |
| GGCATCACCATAAAGAAAAC+CGG | - | chr4.3:3170079-3170098 | MS.gene015837:CDS | 40.0% | |
| GTCAAATACATGGTCATCCA+AGG | - | chr4.3:3169757-3169776 | MS.gene015837:intron | 40.0% | |
| TTATTTGGATAGGGATCTGG+AGG | - | chr4.3:3170058-3170077 | MS.gene015837:intron | 40.0% | |
| ! | ATGTGTGATATTGATGGCAC+CGG | - | chr4.3:3169047-3169066 | MS.gene015837:CDS | 40.0% |
| !! | AGCTTGACCGGTTTTCTTTA+TGG | + | chr4.3:3170089-3170108 | None:intergenic | 40.0% |
| ACAACCATGTTGCATTGTCC+AGG | + | chr4.3:3170145-3170164 | None:intergenic | 45.0% | |
| ACCGAAAACTAGAGCTTGAC+CGG | + | chr4.3:3170101-3170120 | None:intergenic | 45.0% | |
| CAACATGGTTGTTGAGAGGT+TGG | - | chr4.3:3170153-3170172 | MS.gene015837:CDS | 45.0% | |
| CCATGTATTTGACTCCTGCA+AGG | + | chr4.3:3169750-3169769 | None:intergenic | 45.0% | |
| CCTTGCAGGAGTCAAATACA+TGG | - | chr4.3:3169747-3169766 | MS.gene015837:intron | 45.0% | |
| GGGAGATTACCTCATTGATC+AGG | - | chr4.3:3170174-3170193 | MS.gene015837:CDS | 45.0% | |
| GTTTGAGTACCCTCGTCTTA+TGG | - | chr4.3:3169917-3169936 | MS.gene015837:intron | 45.0% | |
| !!! | ACCGGTCAAGCTCTAGTTTT+CGG | - | chr4.3:3170097-3170116 | MS.gene015837:CDS | 45.0% |
| ACCTGGTCATCTTGCTCCTA+CGG | - | chr4.3:3169717-3169736 | MS.gene015837:intron | 50.0% | |
| AGAGCCATCATGGCCAATGA+TGG | + | chr4.3:3169093-3169112 | None:intergenic | 50.0% | |
| ATCATGGCCAATGATGGCAG+CGG | + | chr4.3:3169087-3169106 | None:intergenic | 50.0% | |
| ATGGTCATCCAAGGAGAGTC+TGG | - | chr4.3:3169766-3169785 | MS.gene015837:intron | 50.0% | |
| GACTCCTGGACAATGCAACA+TGG | - | chr4.3:3170138-3170157 | MS.gene015837:CDS | 50.0% | |
| TATGAGGAACCTGTGACTCC+TGG | - | chr4.3:3170124-3170143 | MS.gene015837:CDS | 50.0% | |
| TTGCATTGTCCAGGAGTCAC+AGG | + | chr4.3:3170136-3170155 | None:intergenic | 50.0% | |
| ! | TGGCCATGATGGCTCTGTTT+GGG | - | chr4.3:3169097-3169116 | MS.gene015837:CDS | 50.0% |
| ! | TTGGCCATGATGGCTCTGTT+TGG | - | chr4.3:3169096-3169115 | MS.gene015837:CDS | 50.0% |
| AGCTGTCATCCGTGGGAAGA+AGG | - | chr4.3:3169789-3169808 | MS.gene015837:intron | 55.0% | |
| CAATGATGGCAGCGGCAGAT+AGG | + | chr4.3:3169079-3169098 | None:intergenic | 55.0% | |
| CCTGGTCATCTTGCTCCTAC+GGG | - | chr4.3:3169718-3169737 | MS.gene015837:intron | 55.0% | |
| CTATCTGCCGCTGCCATCAT+TGG | - | chr4.3:3169077-3169096 | MS.gene015837:CDS | 55.0% | |
| GAGCCCAAACAGAGCCATCA+TGG | + | chr4.3:3169103-3169122 | None:intergenic | 55.0% | |
| GCTGCCATCATTGGCCATGA+TGG | - | chr4.3:3169086-3169105 | MS.gene015837:CDS | 55.0% | |
| TGACAGCTCCAGACTCTCCT+TGG | + | chr4.3:3169777-3169796 | None:intergenic | 55.0% | |
| ! | AGTCTGGAGCTGTCATCCGT+GGG | - | chr4.3:3169782-3169801 | MS.gene015837:intron | 55.0% |
| AGCGGCAGATAGGTGGTGTC+CGG | + | chr4.3:3169069-3169088 | None:intergenic | 60.0% | |
| CCCGTAGGAGCAAGATGACC+AGG | + | chr4.3:3169721-3169740 | None:intergenic | 60.0% | |
| TGATGGCAGCGGCAGATAGG+TGG | + | chr4.3:3169076-3169095 | None:intergenic | 60.0% | |
| ! | GAGTCTGGAGCTGTCATCCG+TGG | - | chr4.3:3169781-3169800 | MS.gene015837:intron | 60.0% |
| CCTACGGGCTTGCACCTTGC+AGG | - | chr4.3:3169733-3169752 | MS.gene015837:intron | 65.0% | |
| CCTGCAAGGTGCAAGCCCGT+AGG | + | chr4.3:3169736-3169755 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 3169014 | 3170204 | 3169014 | ID=MS.gene015837 |
| chr4.3 | mRNA | 3169014 | 3170204 | 3169014 | ID=MS.gene015837.t1;Parent=MS.gene015837 |
| chr4.3 | exon | 3170076 | 3170204 | 3170076 | ID=MS.gene015837.t1.exon1;Parent=MS.gene015837.t1 |
| chr4.3 | CDS | 3170076 | 3170204 | 3170076 | ID=cds.MS.gene015837.t1;Parent=MS.gene015837.t1 |
| chr4.3 | exon | 3169408 | 3169545 | 3169408 | ID=MS.gene015837.t1.exon2;Parent=MS.gene015837.t1 |
| chr4.3 | CDS | 3169408 | 3169545 | 3169408 | ID=cds.MS.gene015837.t1;Parent=MS.gene015837.t1 |
| chr4.3 | exon | 3169014 | 3169148 | 3169014 | ID=MS.gene015837.t1.exon3;Parent=MS.gene015837.t1 |
| chr4.3 | CDS | 3169014 | 3169148 | 3169014 | ID=cds.MS.gene015837.t1;Parent=MS.gene015837.t1 |
| Gene Sequence |
| Protein sequence |
