Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049055.t1 | XP_024628281.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.60E-71 | 278.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049055.t1 | A4K9Z8 | 90.2 | 133 | 13 | 0 | 1 | 133 | 1 | 133 | 8.5e-69 | 260.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049055.t1 | A0A396GR17 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.2e-71 | 278.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044849 | MS.gene049055 | PPI |
| MS.gene049055 | MS.gene20134 | PPI |
| MS.gene20133 | MS.gene049055 | PPI |
| MS.gene27990 | MS.gene049055 | PPI |
| MS.gene049056 | MS.gene049055 | PPI |
| MS.gene015837 | MS.gene049055 | PPI |
| MS.gene001874 | MS.gene049055 | PPI |
| MS.gene015836 | MS.gene049055 | PPI |
| MS.gene049055 | MS.gene05946 | PPI |
| MS.gene05947 | MS.gene049055 | PPI |
| MS.gene030883 | MS.gene049055 | PPI |
| MS.gene79962 | MS.gene049055 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049055.t1 | MTR_6g090030 | 89.474 | 133 | 14 | 0 | 1 | 133 | 1 | 133 | 6.18e-88 | 252 |
| MS.gene049055.t1 | MTR_8g102330 | 84.211 | 133 | 21 | 0 | 1 | 133 | 1 | 133 | 1.91e-83 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049055.t1 | AT2G19770 | 86.567 | 134 | 17 | 1 | 1 | 133 | 1 | 134 | 1.37e-85 | 246 |
| MS.gene049055.t1 | AT4G29340 | 84.328 | 134 | 20 | 1 | 1 | 133 | 1 | 134 | 2.46e-83 | 240 |
| MS.gene049055.t1 | AT2G19760 | 75.188 | 133 | 31 | 1 | 1 | 133 | 1 | 131 | 2.03e-71 | 210 |
| MS.gene049055.t1 | AT4G29350 | 73.684 | 133 | 33 | 1 | 1 | 133 | 1 | 131 | 1.18e-70 | 208 |
| MS.gene049055.t1 | AT5G56600 | 72.180 | 133 | 35 | 1 | 1 | 133 | 1 | 131 | 6.70e-69 | 204 |
| MS.gene049055.t1 | AT5G56600 | 70.787 | 89 | 24 | 1 | 1 | 89 | 1 | 87 | 2.99e-41 | 133 |
Find 42 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGCCATGATGGCTCTGTT+TGG | 0.298573 | 4.2:-2783053 | MS.gene049055:CDS |
| CCTACGGGCTTGCACCTTGC+AGG | 0.315777 | 4.2:-2782416 | MS.gene049055:CDS |
| TGGCCATGATGGCTCTGTTT+GGG | 0.344130 | 4.2:-2783052 | MS.gene049055:CDS |
| CTATCTGCCGCTGCCATCAT+TGG | 0.366810 | 4.2:-2783072 | MS.gene049055:CDS |
| ATGGTCATCCAAGGAGAGTC+TGG | 0.390726 | 4.2:-2782383 | MS.gene049055:CDS |
| GGGAGATTACCTCATTGATC+AGG | 0.391286 | 4.2:-2781975 | MS.gene049055:CDS |
| TTGTTATTTGGATAGGGATC+TGG | 0.425299 | 4.2:-2782094 | MS.gene049055:intron |
| CAATGATGGCAGCGGCAGAT+AGG | 0.436163 | 4.2:+2783073 | None:intergenic |
| ACCTGGTCATCTTGCTCCTA+CGG | 0.438799 | 4.2:-2782432 | MS.gene049055:CDS |
| TGACAGCTCCAGACTCTCCT+TGG | 0.476205 | 4.2:+2782375 | None:intergenic |
| AGCGGCAGATAGGTGGTGTC+CGG | 0.488939 | 4.2:+2783083 | None:intergenic |
| CCTGGTCATCTTGCTCCTAC+GGG | 0.490248 | 4.2:-2782431 | MS.gene049055:CDS |
| GCTGCCATCATTGGCCATGA+TGG | 0.491650 | 4.2:-2783063 | MS.gene049055:CDS |
| GGCATCACCATAAAGAAAAC+CGG | 0.492694 | 4.2:-2782070 | MS.gene049055:CDS |
| CATTTGATGTGTGATATTGA+TGG | 0.492739 | 4.2:-2783108 | MS.gene049055:CDS |
| TTGCATTGTCCAGGAGTCAC+AGG | 0.497282 | 4.2:+2782016 | None:intergenic |
| ACCGAAAACTAGAGCTTGAC+CGG | 0.513054 | 4.2:+2782051 | None:intergenic |
| CAACATGGTTGTTGAGAGGT+TGG | 0.513380 | 4.2:-2781996 | MS.gene049055:CDS |
| ACAACCATGTTGCATTGTCC+AGG | 0.519180 | 4.2:+2782007 | None:intergenic |
| AGCTGTCATCCGTGGGAAGA+AGG | 0.522760 | 4.2:-2782360 | MS.gene049055:intron |
| TATGAGGAACCTGTGACTCC+TGG | 0.523087 | 4.2:-2782025 | MS.gene049055:CDS |
| GAGCCCAAACAGAGCCATCA+TGG | 0.526888 | 4.2:+2783049 | None:intergenic |
| CCCGTAGGAGCAAGATGACC+AGG | 0.528322 | 4.2:+2782431 | None:intergenic |
| ATAATATCAGTGATCTCTTG+AGG | 0.540089 | 4.2:+2782470 | None:intergenic |
| AGAGCCATCATGGCCAATGA+TGG | 0.541950 | 4.2:+2783059 | None:intergenic |
| AACATGGTTGTTGAGAGGTT+GGG | 0.543359 | 4.2:-2781995 | MS.gene049055:CDS |
| GAGTCTGGAGCTGTCATCCG+TGG | 0.545615 | 4.2:-2782368 | MS.gene049055:CDS |
| GTCAAATACATGGTCATCCA+AGG | 0.546015 | 4.2:-2782392 | MS.gene049055:CDS |
| TTATTTGGATAGGGATCTGG+AGG | 0.562519 | 4.2:-2782091 | MS.gene049055:intron |
| AATAATTTACCTTCTTCCCA+CGG | 0.573845 | 4.2:+2782351 | None:intergenic |
| GGAGATTACCTCATTGATCA+GGG | 0.584642 | 4.2:-2781974 | MS.gene049055:CDS |
| CCTGCAAGGTGCAAGCCCGT+AGG | 0.594563 | 4.2:+2782416 | None:intergenic |
| ATGTGTGATATTGATGGCAC+CGG | 0.603576 | 4.2:-2783102 | MS.gene049055:CDS |
| CCTTGCAGGAGTCAAATACA+TGG | 0.605937 | 4.2:-2782402 | MS.gene049055:CDS |
| ATCATGGCCAATGATGGCAG+CGG | 0.611873 | 4.2:+2783065 | None:intergenic |
| TCATGAATATACTTGAGGAA+AGG | 0.612766 | 4.2:+2783017 | None:intergenic |
| TGATGGCAGCGGCAGATAGG+TGG | 0.614292 | 4.2:+2783076 | None:intergenic |
| GACTCCTGGACAATGCAACA+TGG | 0.634894 | 4.2:-2782011 | MS.gene049055:CDS |
| AGTCTGGAGCTGTCATCCGT+GGG | 0.647697 | 4.2:-2782367 | MS.gene049055:CDS |
| AATGCAACATGGTTGTTGAG+AGG | 0.662368 | 4.2:-2782000 | MS.gene049055:CDS |
| TCTACAGGCCCTGATCAATG+AGG | 0.683059 | 4.2:+2781966 | None:intergenic |
| CCATGTATTTGACTCCTGCA+AGG | 0.700396 | 4.2:+2782402 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAACATTGTAATTAAAA+TGG | + | chr4.2:2782393-2782412 | None:intergenic | 15.0% |
| !! | TTTGAAGATTAGATTTAATT+AGG | - | chr4.2:2782532-2782551 | MS.gene049055:intron | 15.0% |
| !!! | ATTTTGAAAGTAATTATACT+TGG | - | chr4.2:2782261-2782280 | MS.gene049055:intron | 15.0% |
| !! | ATCATCATTGTAAAGATTTA+CGG | + | chr4.2:2782326-2782345 | None:intergenic | 20.0% |
| ! | GAAGATTAGATTTAATTAGG+AGG | - | chr4.2:2782535-2782554 | MS.gene049055:intron | 25.0% |
| ! | TGTAATCATGAATATACTTG+AGG | + | chr4.2:2782093-2782112 | None:intergenic | 25.0% |
| !! | TATGTAAGATCGATTTAGAA+AGG | + | chr4.2:2782471-2782490 | None:intergenic | 25.0% |
| !!! | TGATATATGTGCTTGTTATT+TGG | - | chr4.2:2782996-2783015 | MS.gene049055:intron | 25.0% |
| AAAAAAGAGACATGTACGTT+AGG | + | chr4.2:2782957-2782976 | None:intergenic | 30.0% | |
| AATAATTTACCTTCTTCCCA+CGG | + | chr4.2:2782754-2782773 | None:intergenic | 30.0% | |
| ATAATATCAGTGATCTCTTG+AGG | + | chr4.2:2782635-2782654 | None:intergenic | 30.0% | |
| ATGCATAGAATAGTTTCATG+TGG | + | chr4.2:2782119-2782138 | None:intergenic | 30.0% | |
| CATTTGATGTGTGATATTGA+TGG | - | chr4.2:2781994-2782013 | MS.gene049055:CDS | 30.0% | |
| TCATGAATATACTTGAGGAA+AGG | + | chr4.2:2782088-2782107 | None:intergenic | 30.0% | |
| TGCATAGAATAGTTTCATGT+GGG | + | chr4.2:2782118-2782137 | None:intergenic | 30.0% | |
| TTCAAAATCACACTATGATC+TGG | + | chr4.2:2782249-2782268 | None:intergenic | 30.0% | |
| !! | ATGTGCTTGTTATTTGGATA+GGG | - | chr4.2:2783002-2783021 | MS.gene049055:intron | 30.0% |
| !! | TATGTGCTTGTTATTTGGAT+AGG | - | chr4.2:2783001-2783020 | MS.gene049055:intron | 30.0% |
| AAAAGTTAGTCCATAAGACG+AGG | + | chr4.2:2782883-2782902 | None:intergenic | 35.0% | |
| AAAGTTAGTCCATAAGACGA+GGG | + | chr4.2:2782882-2782901 | None:intergenic | 35.0% | |
| GCATAGATCTCTATCATTCT+TGG | - | chr4.2:2782135-2782154 | MS.gene049055:intron | 35.0% | |
| GTTGTATTTGAGTCTCATGT+TGG | - | chr4.2:2782813-2782832 | MS.gene049055:intron | 35.0% | |
| !! | ATGAAAGATTTTGACGAACC+TGG | - | chr4.2:2782653-2782672 | MS.gene049055:intron | 35.0% |
| !! | TACGTTAGGTTTTATGCATG+TGG | + | chr4.2:2782943-2782962 | None:intergenic | 35.0% |
| !! | TTGTTATTTGGATAGGGATC+TGG | - | chr4.2:2783008-2783027 | MS.gene049055:intron | 35.0% |
| !!! | TCTAGTTTTCGGTGTTTATG+AGG | - | chr4.2:2783061-2783080 | MS.gene049055:CDS | 35.0% |
| AACATGGTTGTTGAGAGGTT+GGG | - | chr4.2:2783107-2783126 | MS.gene049055:CDS | 40.0% | |
| AATGCAACATGGTTGTTGAG+AGG | - | chr4.2:2783102-2783121 | MS.gene049055:CDS | 40.0% | |
| GGAGATTACCTCATTGATCA+GGG | - | chr4.2:2783128-2783147 | MS.gene049055:CDS | 40.0% | |
| GGCATCACCATAAAGAAAAC+CGG | - | chr4.2:2783032-2783051 | MS.gene049055:CDS | 40.0% | |
| GTCAAATACATGGTCATCCA+AGG | - | chr4.2:2782710-2782729 | MS.gene049055:intron | 40.0% | |
| TTATTTGGATAGGGATCTGG+AGG | - | chr4.2:2783011-2783030 | MS.gene049055:intron | 40.0% | |
| ! | ATGTGTGATATTGATGGCAC+CGG | - | chr4.2:2782000-2782019 | MS.gene049055:CDS | 40.0% |
| !! | AGCTTGACCGGTTTTCTTTA+TGG | + | chr4.2:2783042-2783061 | None:intergenic | 40.0% |
| ACAACCATGTTGCATTGTCC+AGG | + | chr4.2:2783098-2783117 | None:intergenic | 45.0% | |
| ACCGAAAACTAGAGCTTGAC+CGG | + | chr4.2:2783054-2783073 | None:intergenic | 45.0% | |
| CAACATGGTTGTTGAGAGGT+TGG | - | chr4.2:2783106-2783125 | MS.gene049055:CDS | 45.0% | |
| CCATGTATTTGACTCCTGCA+AGG | + | chr4.2:2782703-2782722 | None:intergenic | 45.0% | |
| CCTTGCAGGAGTCAAATACA+TGG | - | chr4.2:2782700-2782719 | MS.gene049055:intron | 45.0% | |
| GGGAGATTACCTCATTGATC+AGG | - | chr4.2:2783127-2783146 | MS.gene049055:CDS | 45.0% | |
| GTTTGAGTACCCTCGTCTTA+TGG | - | chr4.2:2782870-2782889 | MS.gene049055:intron | 45.0% | |
| !!! | ACCGGTCAAGCTCTAGTTTT+CGG | - | chr4.2:2783050-2783069 | MS.gene049055:CDS | 45.0% |
| ACCTGGTCATCTTGCTCCTA+CGG | - | chr4.2:2782670-2782689 | MS.gene049055:intron | 50.0% | |
| AGAGCCATCATGGCCAATGA+TGG | + | chr4.2:2782046-2782065 | None:intergenic | 50.0% | |
| ATCATGGCCAATGATGGCAG+CGG | + | chr4.2:2782040-2782059 | None:intergenic | 50.0% | |
| ATGGTCATCCAAGGAGAGTC+TGG | - | chr4.2:2782719-2782738 | MS.gene049055:intron | 50.0% | |
| GACTCCTGGACAATGCAACA+TGG | - | chr4.2:2783091-2783110 | MS.gene049055:CDS | 50.0% | |
| TATGAGGAACCTGTGACTCC+TGG | - | chr4.2:2783077-2783096 | MS.gene049055:CDS | 50.0% | |
| TTGCATTGTCCAGGAGTCAC+AGG | + | chr4.2:2783089-2783108 | None:intergenic | 50.0% | |
| ! | TGGCCATGATGGCTCTGTTT+GGG | - | chr4.2:2782050-2782069 | MS.gene049055:CDS | 50.0% |
| ! | TTGGCCATGATGGCTCTGTT+TGG | - | chr4.2:2782049-2782068 | MS.gene049055:CDS | 50.0% |
| AGCTGTCATCCGTGGGAAGA+AGG | - | chr4.2:2782742-2782761 | MS.gene049055:intron | 55.0% | |
| CAATGATGGCAGCGGCAGAT+AGG | + | chr4.2:2782032-2782051 | None:intergenic | 55.0% | |
| CCTGGTCATCTTGCTCCTAC+GGG | - | chr4.2:2782671-2782690 | MS.gene049055:intron | 55.0% | |
| CTATCTGCCGCTGCCATCAT+TGG | - | chr4.2:2782030-2782049 | MS.gene049055:CDS | 55.0% | |
| GAGCCCAAACAGAGCCATCA+TGG | + | chr4.2:2782056-2782075 | None:intergenic | 55.0% | |
| GCTGCCATCATTGGCCATGA+TGG | - | chr4.2:2782039-2782058 | MS.gene049055:CDS | 55.0% | |
| TGACAGCTCCAGACTCTCCT+TGG | + | chr4.2:2782730-2782749 | None:intergenic | 55.0% | |
| ! | AGTCTGGAGCTGTCATCCGT+GGG | - | chr4.2:2782735-2782754 | MS.gene049055:intron | 55.0% |
| AGCGGCAGATAGGTGGTGTC+CGG | + | chr4.2:2782022-2782041 | None:intergenic | 60.0% | |
| CCCGTAGGAGCAAGATGACC+AGG | + | chr4.2:2782674-2782693 | None:intergenic | 60.0% | |
| TGATGGCAGCGGCAGATAGG+TGG | + | chr4.2:2782029-2782048 | None:intergenic | 60.0% | |
| ! | GAGTCTGGAGCTGTCATCCG+TGG | - | chr4.2:2782734-2782753 | MS.gene049055:intron | 60.0% |
| CCTACGGGCTTGCACCTTGC+AGG | - | chr4.2:2782686-2782705 | MS.gene049055:intron | 65.0% | |
| CCTGCAAGGTGCAAGCCCGT+AGG | + | chr4.2:2782689-2782708 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 2781967 | 2783157 | 2781967 | ID=MS.gene049055 |
| chr4.2 | mRNA | 2781967 | 2783157 | 2781967 | ID=MS.gene049055.t1;Parent=MS.gene049055 |
| chr4.2 | exon | 2783029 | 2783157 | 2783029 | ID=MS.gene049055.t1.exon1;Parent=MS.gene049055.t1 |
| chr4.2 | CDS | 2783029 | 2783157 | 2783029 | ID=cds.MS.gene049055.t1;Parent=MS.gene049055.t1 |
| chr4.2 | exon | 2782361 | 2782498 | 2782361 | ID=MS.gene049055.t1.exon2;Parent=MS.gene049055.t1 |
| chr4.2 | CDS | 2782361 | 2782498 | 2782361 | ID=cds.MS.gene049055.t1;Parent=MS.gene049055.t1 |
| chr4.2 | exon | 2781967 | 2782101 | 2781967 | ID=MS.gene049055.t1.exon3;Parent=MS.gene049055.t1 |
| chr4.2 | CDS | 2781967 | 2782101 | 2781967 | ID=cds.MS.gene049055.t1;Parent=MS.gene049055.t1 |
| Gene Sequence |
| Protein sequence |
