Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05946.t1 | AFK40156.1 | 98.5 | 131 | 2 | 0 | 1 | 131 | 1 | 131 | 1.80E-70 | 275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05946.t1 | P49231 | 88.5 | 131 | 15 | 0 | 1 | 131 | 1 | 131 | 1.3e-66 | 253.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05946.t1 | I3SIR4 | 98.5 | 131 | 2 | 0 | 1 | 131 | 1 | 131 | 1.3e-70 | 275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05946 | MS.gene20133 | 0.977033 | 5.61E-143 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene05946 | MS.gene024155 | PPI |
| MS.gene20133 | MS.gene05946 | PPI |
| MS.gene05946 | MS.gene20134 | PPI |
| MS.gene033590 | MS.gene05946 | PPI |
| MS.gene049056 | MS.gene05946 | PPI |
| MS.gene049055 | MS.gene05946 | PPI |
| MS.gene015837 | MS.gene05946 | PPI |
| MS.gene015836 | MS.gene05946 | PPI |
| MS.gene55068 | MS.gene05946 | PPI |
| MS.gene065414 | MS.gene05946 | PPI |
| MS.gene05946 | MS.gene86676 | PPI |
| MS.gene05947 | MS.gene05946 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05946.t1 | MTR_8g102330 | 89.474 | 133 | 12 | 1 | 1 | 131 | 1 | 133 | 8.58e-86 | 246 |
| MS.gene05946.t1 | MTR_6g090030 | 78.947 | 133 | 26 | 1 | 1 | 131 | 1 | 133 | 9.62e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05946.t1 | AT2G19760 | 79.389 | 131 | 27 | 0 | 1 | 131 | 1 | 131 | 8.93e-76 | 221 |
| MS.gene05946.t1 | AT5G56600 | 79.389 | 131 | 27 | 0 | 1 | 131 | 1 | 131 | 9.53e-76 | 221 |
| MS.gene05946.t1 | AT4G29350 | 77.863 | 131 | 29 | 0 | 1 | 131 | 1 | 131 | 1.48e-75 | 220 |
| MS.gene05946.t1 | AT2G19770 | 76.119 | 134 | 29 | 1 | 1 | 131 | 1 | 134 | 9.55e-75 | 218 |
| MS.gene05946.t1 | AT4G29340 | 75.373 | 134 | 30 | 1 | 1 | 131 | 1 | 134 | 1.89e-72 | 213 |
| MS.gene05946.t1 | AT5G56600 | 78.161 | 87 | 19 | 0 | 1 | 87 | 1 | 87 | 3.36e-46 | 145 |
Find 0 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAATTTAATAGAGAAAA+TGG | + | 56947:49011-49030 | None:intergenic | 10.0% |
| !! | CTGTTAATTAATTAATTAAG+TGG | - | 56947:48324-48343 | MS.gene05946:intron | 15.0% |
| !!! | AATTTCTGAATACTTATTTT+GGG | - | 56947:49324-49343 | MS.gene05946:intron | 15.0% |
| !!! | TAATTTCTGAATACTTATTT+TGG | - | 56947:49323-49342 | MS.gene05946:intron | 15.0% |
| !!! | TATTTTAATCTTTCAAATTG+AGG | - | 56947:49112-49131 | MS.gene05946:intron | 15.0% |
| !! | ATTGAAAATCTATTTGAAAG+TGG | - | 56947:49030-49049 | MS.gene05946:intron | 20.0% |
| !! | TTGAAAATCTATTTGAAAGT+GGG | - | 56947:49031-49050 | MS.gene05946:intron | 20.0% |
| !!! | AACAGTTAAATGCTATTTAT+TGG | - | 56947:49176-49195 | MS.gene05946:intron | 20.0% |
| !!! | AATGCCATTTTACTATTTTA+GGG | - | 56947:48902-48921 | MS.gene05946:CDS | 20.0% |
| !!! | TGGTCAATTTTTTTATTCAA+TGG | - | 56947:49249-49268 | MS.gene05946:intron | 20.0% |
| ! | AAACTGCTCTATTTAATTGT+AGG | - | 56947:48702-48721 | MS.gene05946:intron | 25.0% |
| ! | AATAGCATTTAACTGTTCAA+AGG | + | 56947:49173-49192 | None:intergenic | 25.0% |
| ! | AATTTCAAAAAGACCAAACA+TGG | + | 56947:48734-48753 | None:intergenic | 25.0% |
| ! | ACAATTTCAGAAATCACAAT+GGG | - | 56947:48973-48992 | MS.gene05946:CDS | 25.0% |
| ! | ACATCCCTAAAATAGTAAAA+TGG | + | 56947:48909-48928 | None:intergenic | 25.0% |
| ! | ACTAAATATATGGTGATTCA+AGG | - | 56947:48543-48562 | MS.gene05946:intron | 25.0% |
| ! | ATTGATCTGAATAAGTCTTA+TGG | - | 56947:49229-49248 | MS.gene05946:intron | 25.0% |
| ! | CTTATTCAGATCAATATTGA+AGG | + | 56947:49224-49243 | None:intergenic | 25.0% |
| ! | GAGATTCAATCTAACATAAT+AGG | + | 56947:48681-48700 | None:intergenic | 25.0% |
| ! | TACAATTTCAGAAATCACAA+TGG | - | 56947:48972-48991 | MS.gene05946:CDS | 25.0% |
| ! | TTATACATTGTGAAGTGTAA+TGG | - | 56947:48364-48383 | MS.gene05946:intron | 25.0% |
| !! | GTTCTTTATTTGTAATGTGT+TGG | - | 56947:48417-48436 | MS.gene05946:intron | 25.0% |
| !! | TAACCTTACACTGATTATTA+AGG | - | 56947:48230-48249 | MS.gene05946:intron | 25.0% |
| !! | TATTGGTGGTACTAAATATA+TGG | - | 56947:48533-48552 | MS.gene05946:intron | 25.0% |
| !!! | GAATGCCATTTTACTATTTT+AGG | - | 56947:48901-48920 | MS.gene05946:CDS | 25.0% |
| !!! | GTTTGGTCTTTTTGAAATTA+AGG | - | 56947:48735-48754 | MS.gene05946:intron | 25.0% |
| !!! | TTGAAGGTAATTTTTTATGC+AGG | + | 56947:49208-49227 | None:intergenic | 25.0% |
| AGTCAAATTCTCTGATGAAT+CGG | + | 56947:48290-48309 | None:intergenic | 30.0% | |
| ATATAAGCAAGTTATTGTGC+AGG | + | 56947:49304-49323 | None:intergenic | 30.0% | |
| CAAATGAAGTTCTGTACTTA+GGG | - | 56947:48262-48281 | MS.gene05946:intron | 30.0% | |
| GATCCTTAATAATCAGTGTA+AGG | + | 56947:48236-48255 | None:intergenic | 30.0% | |
| GTTGTCAATTTAGGAATTTC+AGG | - | 56947:48930-48949 | MS.gene05946:CDS | 30.0% | |
| TACTGTTAAGAAGACCAATA+TGG | - | 56947:49377-49396 | MS.gene05946:CDS | 30.0% | |
| !! | CATTTTAGTCACACATTTCA+TGG | + | 56947:48882-48901 | None:intergenic | 30.0% |
| AGGGATGTTGTTGTCAATTT+AGG | - | 56947:48921-48940 | MS.gene05946:CDS | 35.0% | |
| ATCATAAATGCCAATGACCA+AGG | + | 56947:49403-49422 | None:intergenic | 35.0% | |
| ATCTATGCTATGCTATGCTT+TGG | - | 56947:48645-48664 | MS.gene05946:intron | 35.0% | |
| GCAAATGAAGTTCTGTACTT+AGG | - | 56947:48261-48280 | MS.gene05946:intron | 35.0% | |
| GGTGATTATCTTGTTGAACA+GGG | - | 56947:49468-49487 | MS.gene05946:CDS | 35.0% | |
| TGGTGATTATCTTGTTGAAC+AGG | - | 56947:49467-49486 | MS.gene05946:CDS | 35.0% | |
| TTGGGCATTATATTATCCCA+GGG | - | 56947:49342-49361 | MS.gene05946:intron | 35.0% | |
| TTTGGGCATTATATTATCCC+AGG | - | 56947:49341-49360 | MS.gene05946:intron | 35.0% | |
| ! | TTGATAGCAGTTATCTCTTC+AGG | + | 56947:48468-48487 | None:intergenic | 35.0% |
| AACATGGTAGTTGAAAGGCT+TGG | - | 56947:49447-49466 | MS.gene05946:CDS | 40.0% | |
| AATAAAGACTTCGATGAGCC+TGG | - | 56947:48486-48505 | MS.gene05946:intron | 40.0% | |
| AGTGCAACATGGTAGTTGAA+AGG | - | 56947:49442-49461 | MS.gene05946:CDS | 40.0% | |
| ATTATATTATCCCAGGGTGC+TGG | - | 56947:49348-49367 | MS.gene05946:intron | 40.0% | |
| CCAACTGGATTACATATTGG+TGG | - | 56947:48519-48538 | MS.gene05946:intron | 40.0% | |
| CCAATCACAGTAACAGAGAT+CGG | + | 56947:48206-48225 | None:intergenic | 40.0% | |
| CCACCAATATGTAATCCAGT+TGG | + | 56947:48522-48541 | None:intergenic | 40.0% | |
| GCTCCAACTGGATTACATAT+TGG | - | 56947:48516-48535 | MS.gene05946:intron | 40.0% | |
| TAAGAAGACCAATATGGCCT+TGG | - | 56947:49383-49402 | MS.gene05946:CDS | 40.0% | |
| TACCTTCAATGTCACAGAGA+AGG | + | 56947:48021-48040 | None:intergenic | 40.0% | |
| TATGATGAACCAATGACTCC+CGG | - | 56947:49417-49436 | MS.gene05946:CDS | 40.0% | |
| TTGTAGGTTGTTGCCATGTT+TGG | - | 56947:48718-48737 | MS.gene05946:intron | 40.0% | |
| ! | CACAAGCATTGCCAATGATA+AGG | + | 56947:49063-49082 | None:intergenic | 40.0% |
| !! | CAGATCGATGGATTGATGAA+AGG | + | 56947:48118-48137 | None:intergenic | 40.0% |
| !! | GATTGATGAAAGGTACCTGA+GGG | + | 56947:48108-48127 | None:intergenic | 40.0% |
| ATGGTGATTCAAGGTGAACC+TGG | - | 56947:48552-48571 | MS.gene05946:intron | 45.0% | |
| CACCTTCTCTGTGACATTGA+AGG | - | 56947:48016-48035 | MS.gene05946:CDS | 45.0% | |
| CCGATCTCTGTTACTGTGAT+TGG | - | 56947:48203-48222 | MS.gene05946:intron | 45.0% | |
| TCGCGAAAGAAACAGATCGA+TGG | + | 56947:48130-48149 | None:intergenic | 45.0% | |
| ! | AACTACCATGTTGCACTGAC+CGG | + | 56947:49439-49458 | None:intergenic | 45.0% |
| ! | ACCAATATGGCCTTGGTCAT+TGG | - | 56947:49390-49409 | MS.gene05946:CDS | 45.0% |
| ! | GTGGGTTGCAACCTTATCAT+TGG | - | 56947:49049-49068 | MS.gene05946:intron | 45.0% |
| ! | TCAGAGTGCTAATTTCCCTC+AGG | - | 56947:48090-48109 | MS.gene05946:CDS | 45.0% |
| !! | GGATTGATGAAAGGTACCTG+AGG | + | 56947:48109-48128 | None:intergenic | 45.0% |
| AACCTGGAGCTGTCATTCGA+GGG | - | 56947:48568-48587 | MS.gene05946:intron | 50.0% | |
| AGCTGTCATTCGAGGGAAGA+AGG | - | 56947:48575-48594 | MS.gene05946:intron | 50.0% | |
| CTCACTCATGCTGCTATCCT+CGG | - | 56947:48046-48065 | MS.gene05946:CDS | 50.0% | |
| GCCAATGACCAAGGCCATAT+TGG | + | 56947:49394-49413 | None:intergenic | 50.0% | |
| ! | ACTACCATGTTGCACTGACC+GGG | + | 56947:49438-49457 | None:intergenic | 50.0% |
| !! | ATATTATCCCAGGGTGCTGG+TGG | - | 56947:49351-49370 | MS.gene05946:intron | 50.0% |
| AAACGCTACCGTCTACACCG+AGG | + | 56947:48066-48085 | None:intergenic | 55.0% | |
| AGTAACACCACCAGCACCCT+GGG | + | 56947:49361-49380 | None:intergenic | 55.0% | |
| CCAGTTGGAGCAAGAGTTCC+AGG | + | 56947:48507-48526 | None:intergenic | 55.0% | |
| CCTGGAACTCTTGCTCCAAC+TGG | - | 56947:48504-48523 | MS.gene05946:intron | 55.0% | |
| CGGTGTAGACGGTAGCGTTT+GGG | - | 56947:48066-48085 | MS.gene05946:CDS | 55.0% | |
| GAACCTGGAGCTGTCATTCG+AGG | - | 56947:48567-48586 | MS.gene05946:intron | 55.0% | |
| TCGGTGTAGACGGTAGCGTT+TGG | - | 56947:48065-48084 | MS.gene05946:CDS | 55.0% | |
| TTCCCTCGAATGACAGCTCC+AGG | + | 56947:48573-48592 | None:intergenic | 55.0% | |
| ! | GCTGCTATCCTCGGTGTAGA+CGG | - | 56947:48055-48074 | MS.gene05946:CDS | 55.0% |
| ! | TTGCACTGACCGGGAGTCAT+TGG | + | 56947:49429-49448 | None:intergenic | 55.0% |
| CAGTAACACCACCAGCACCC+TGG | + | 56947:49362-49381 | None:intergenic | 60.0% | |
| GACTCCCGGTCAGTGCAACA+TGG | - | 56947:49431-49450 | MS.gene05946:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 56947 | gene | 47989 | 49497 | 47989 | ID=MS.gene05946 |
| 56947 | mRNA | 47989 | 49497 | 47989 | ID=MS.gene05946.t1;Parent=MS.gene05946 |
| 56947 | exon | 49375 | 49497 | 49375 | ID=MS.gene05946.t1.exon1;Parent=MS.gene05946.t1 |
| 56947 | CDS | 49375 | 49497 | 49375 | ID=cds.MS.gene05946.t1;Parent=MS.gene05946.t1 |
| 56947 | exon | 48890 | 49027 | 48890 | ID=MS.gene05946.t1.exon2;Parent=MS.gene05946.t1 |
| 56947 | CDS | 48890 | 49027 | 48890 | ID=cds.MS.gene05946.t1;Parent=MS.gene05946.t1 |
| 56947 | exon | 47989 | 48123 | 47989 | ID=MS.gene05946.t1.exon3;Parent=MS.gene05946.t1 |
| 56947 | CDS | 47989 | 48123 | 47989 | ID=cds.MS.gene05946.t1;Parent=MS.gene05946.t1 |
| Gene Sequence |
| Protein sequence |
