Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20134.t1 | XP_024628281.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.60E-71 | 278.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20134.t1 | A4K9Z8 | 90.2 | 133 | 13 | 0 | 1 | 133 | 1 | 133 | 8.5e-69 | 260.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20134.t1 | A0A396GR17 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 1.2e-71 | 278.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05947 | MS.gene20134 | 0.946743 | 2.57E-105 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene049056 | MS.gene20134 | PPI |
| MS.gene049055 | MS.gene20134 | PPI |
| MS.gene015837 | MS.gene20134 | PPI |
| MS.gene001874 | MS.gene20134 | PPI |
| MS.gene015836 | MS.gene20134 | PPI |
| MS.gene05946 | MS.gene20134 | PPI |
| MS.gene05947 | MS.gene20134 | PPI |
| MS.gene79962 | MS.gene20134 | PPI |
| MS.gene044849 | MS.gene20134 | PPI |
| MS.gene030883 | MS.gene20134 | PPI |
| MS.gene20133 | MS.gene20134 | PPI |
| MS.gene27990 | MS.gene20134 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20134.t1 | MTR_6g090030 | 89.474 | 133 | 14 | 0 | 1 | 133 | 1 | 133 | 6.18e-88 | 252 |
| MS.gene20134.t1 | MTR_8g102330 | 84.211 | 133 | 21 | 0 | 1 | 133 | 1 | 133 | 1.91e-83 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20134.t1 | AT2G19770 | 86.567 | 134 | 17 | 1 | 1 | 133 | 1 | 134 | 1.37e-85 | 246 |
| MS.gene20134.t1 | AT4G29340 | 84.328 | 134 | 20 | 1 | 1 | 133 | 1 | 134 | 2.46e-83 | 240 |
| MS.gene20134.t1 | AT2G19760 | 75.188 | 133 | 31 | 1 | 1 | 133 | 1 | 131 | 2.03e-71 | 210 |
| MS.gene20134.t1 | AT4G29350 | 73.684 | 133 | 33 | 1 | 1 | 133 | 1 | 131 | 1.18e-70 | 208 |
| MS.gene20134.t1 | AT5G56600 | 72.180 | 133 | 35 | 1 | 1 | 133 | 1 | 131 | 6.70e-69 | 204 |
| MS.gene20134.t1 | AT5G56600 | 70.787 | 89 | 24 | 1 | 1 | 89 | 1 | 87 | 2.99e-41 | 133 |
Find 42 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGCCATGATGGCTCTGTT+TGG | 0.298573 | 4.4:-3562426 | MS.gene20134:CDS |
| CCTACGGGCTTGCACCTTGC+AGG | 0.315777 | 4.4:-3561789 | MS.gene20134:CDS |
| TGGCCATGATGGCTCTGTTT+GGG | 0.344130 | 4.4:-3562425 | MS.gene20134:CDS |
| CTATCTGCCGCTGCCATCAT+TGG | 0.366810 | 4.4:-3562445 | MS.gene20134:CDS |
| ATGGTCATCCAAGGAGAGTC+TGG | 0.390726 | 4.4:-3561756 | MS.gene20134:CDS |
| GGGAGATTACCTCATTGATC+AGG | 0.391286 | 4.4:-3561348 | MS.gene20134:CDS |
| TTGTTATTTGGATAGGGATC+TGG | 0.425299 | 4.4:-3561467 | MS.gene20134:intron |
| CAATGATGGCAGCGGCAGAT+AGG | 0.436163 | 4.4:+3562446 | None:intergenic |
| ACCTGGTCATCTTGCTCCTA+CGG | 0.438799 | 4.4:-3561805 | MS.gene20134:CDS |
| TGACAGCTCCAGACTCTCCT+TGG | 0.476205 | 4.4:+3561748 | None:intergenic |
| AGCGGCAGATAGGTGGTGTC+CGG | 0.488939 | 4.4:+3562456 | None:intergenic |
| CCTGGTCATCTTGCTCCTAC+GGG | 0.490248 | 4.4:-3561804 | MS.gene20134:CDS |
| GCTGCCATCATTGGCCATGA+TGG | 0.491650 | 4.4:-3562436 | MS.gene20134:CDS |
| GGCATCACCATAAAGAAAAC+CGG | 0.492694 | 4.4:-3561443 | MS.gene20134:CDS |
| CATTTGATGTGTGATATTGA+TGG | 0.492739 | 4.4:-3562481 | MS.gene20134:CDS |
| TTGCATTGTCCAGGAGTCAC+AGG | 0.497282 | 4.4:+3561389 | None:intergenic |
| ACCGAAAACTAGAGCTTGAC+CGG | 0.513054 | 4.4:+3561424 | None:intergenic |
| CAACATGGTTGTTGAGAGGT+TGG | 0.513380 | 4.4:-3561369 | MS.gene20134:CDS |
| ACAACCATGTTGCATTGTCC+AGG | 0.519180 | 4.4:+3561380 | None:intergenic |
| AGCTGTCATCCGTGGGAAGA+AGG | 0.522760 | 4.4:-3561733 | MS.gene20134:intron |
| TATGAGGAACCTGTGACTCC+TGG | 0.523087 | 4.4:-3561398 | MS.gene20134:CDS |
| GAGCCCAAACAGAGCCATCA+TGG | 0.526888 | 4.4:+3562422 | None:intergenic |
| CCCGTAGGAGCAAGATGACC+AGG | 0.528322 | 4.4:+3561804 | None:intergenic |
| ATAATATCAGTGATCTCTTG+AGG | 0.540089 | 4.4:+3561843 | None:intergenic |
| AGAGCCATCATGGCCAATGA+TGG | 0.541950 | 4.4:+3562432 | None:intergenic |
| AACATGGTTGTTGAGAGGTT+GGG | 0.543359 | 4.4:-3561368 | MS.gene20134:CDS |
| GAGTCTGGAGCTGTCATCCG+TGG | 0.545615 | 4.4:-3561741 | MS.gene20134:CDS |
| GTCAAATACATGGTCATCCA+AGG | 0.546015 | 4.4:-3561765 | MS.gene20134:CDS |
| TTATTTGGATAGGGATCTGG+AGG | 0.562519 | 4.4:-3561464 | MS.gene20134:intron |
| AATAATTTACCTTCTTCCCA+CGG | 0.573845 | 4.4:+3561724 | None:intergenic |
| GGAGATTACCTCATTGATCA+GGG | 0.584642 | 4.4:-3561347 | MS.gene20134:CDS |
| CCTGCAAGGTGCAAGCCCGT+AGG | 0.594563 | 4.4:+3561789 | None:intergenic |
| ATGTGTGATATTGATGGCAC+CGG | 0.603576 | 4.4:-3562475 | MS.gene20134:CDS |
| CCTTGCAGGAGTCAAATACA+TGG | 0.605937 | 4.4:-3561775 | MS.gene20134:CDS |
| ATCATGGCCAATGATGGCAG+CGG | 0.611873 | 4.4:+3562438 | None:intergenic |
| TCATGAATATACTTGAGGAA+AGG | 0.612766 | 4.4:+3562390 | None:intergenic |
| TGATGGCAGCGGCAGATAGG+TGG | 0.614292 | 4.4:+3562449 | None:intergenic |
| GACTCCTGGACAATGCAACA+TGG | 0.634894 | 4.4:-3561384 | MS.gene20134:CDS |
| AGTCTGGAGCTGTCATCCGT+GGG | 0.647697 | 4.4:-3561740 | MS.gene20134:CDS |
| AATGCAACATGGTTGTTGAG+AGG | 0.662368 | 4.4:-3561373 | MS.gene20134:CDS |
| TCTACAGGCCCTGATCAATG+AGG | 0.683059 | 4.4:+3561339 | None:intergenic |
| CCATGTATTTGACTCCTGCA+AGG | 0.700396 | 4.4:+3561775 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAACATTGTAATTAAAA+TGG | + | chr4.4:3561766-3561785 | None:intergenic | 15.0% |
| !! | ATCATCATTGTAAAGATTTA+CGG | + | chr4.4:3561699-3561718 | None:intergenic | 20.0% |
| !! | TTTGAAGATTAGATTTGATT+AGG | - | chr4.4:3561905-3561924 | MS.gene20134:intron | 20.0% |
| ! | TGTAATCATGAATATACTTG+AGG | + | chr4.4:3561466-3561485 | None:intergenic | 25.0% |
| !! | TATGTAAGATCGATTTAGAA+AGG | + | chr4.4:3561844-3561863 | None:intergenic | 25.0% |
| !!! | TGATATATGTGCTTGTTATT+TGG | - | chr4.4:3562369-3562388 | MS.gene20134:intron | 25.0% |
| AAAAAAGAGACATGTACGTT+AGG | + | chr4.4:3562330-3562349 | None:intergenic | 30.0% | |
| AATAATTTACCTTCTTCCCA+CGG | + | chr4.4:3562127-3562146 | None:intergenic | 30.0% | |
| ATAATATCAGTGATCTCTTG+AGG | + | chr4.4:3562008-3562027 | None:intergenic | 30.0% | |
| ATGCATAGAATAGTTTCATG+TGG | + | chr4.4:3561492-3561511 | None:intergenic | 30.0% | |
| CATTTGATGTGTGATATTGA+TGG | - | chr4.4:3561367-3561386 | MS.gene20134:CDS | 30.0% | |
| GAAGATTAGATTTGATTAGG+AGG | - | chr4.4:3561908-3561927 | MS.gene20134:intron | 30.0% | |
| TCATGAATATACTTGAGGAA+AGG | + | chr4.4:3561461-3561480 | None:intergenic | 30.0% | |
| TGCATAGAATAGTTTCATGT+GGG | + | chr4.4:3561491-3561510 | None:intergenic | 30.0% | |
| TTCAAAATCACACTATGATC+TGG | + | chr4.4:3561622-3561641 | None:intergenic | 30.0% | |
| !! | ACATGCATTTTTCAAAAGAG+AGG | + | chr4.4:3561731-3561750 | None:intergenic | 30.0% |
| !! | ATGTGCTTGTTATTTGGATA+GGG | - | chr4.4:3562375-3562394 | MS.gene20134:intron | 30.0% |
| !! | TATGTGCTTGTTATTTGGAT+AGG | - | chr4.4:3562374-3562393 | MS.gene20134:intron | 30.0% |
| AAAAGTTAGTCCATAAGACG+AGG | + | chr4.4:3562256-3562275 | None:intergenic | 35.0% | |
| AAAGTTAGTCCATAAGACGA+GGG | + | chr4.4:3562255-3562274 | None:intergenic | 35.0% | |
| GCATAGATCTCTATCATTCT+TGG | - | chr4.4:3561508-3561527 | MS.gene20134:intron | 35.0% | |
| GTTGTATTTGAGTCTCATGT+TGG | - | chr4.4:3562186-3562205 | MS.gene20134:intron | 35.0% | |
| !! | ATGAAAGATTTTGACGAACC+TGG | - | chr4.4:3562026-3562045 | MS.gene20134:intron | 35.0% |
| !! | TACGTTAGGTTTTATGCATG+TGG | + | chr4.4:3562316-3562335 | None:intergenic | 35.0% |
| !! | TTGTTATTTGGATAGGGATC+TGG | - | chr4.4:3562381-3562400 | MS.gene20134:intron | 35.0% |
| !!! | TCTAGTTTTCGGTGTTTATG+AGG | - | chr4.4:3562434-3562453 | MS.gene20134:CDS | 35.0% |
| AACATGGTTGTTGAGAGGTT+GGG | - | chr4.4:3562480-3562499 | MS.gene20134:CDS | 40.0% | |
| AATGCAACATGGTTGTTGAG+AGG | - | chr4.4:3562475-3562494 | MS.gene20134:CDS | 40.0% | |
| GGAGATTACCTCATTGATCA+GGG | - | chr4.4:3562501-3562520 | MS.gene20134:CDS | 40.0% | |
| GGCATCACCATAAAGAAAAC+CGG | - | chr4.4:3562405-3562424 | MS.gene20134:CDS | 40.0% | |
| GTCAAATACATGGTCATCCA+AGG | - | chr4.4:3562083-3562102 | MS.gene20134:intron | 40.0% | |
| TTATTTGGATAGGGATCTGG+AGG | - | chr4.4:3562384-3562403 | MS.gene20134:intron | 40.0% | |
| ! | ATGTGTGATATTGATGGCAC+CGG | - | chr4.4:3561373-3561392 | MS.gene20134:CDS | 40.0% |
| !! | AGCTTGACCGGTTTTCTTTA+TGG | + | chr4.4:3562415-3562434 | None:intergenic | 40.0% |
| ACAACCATGTTGCATTGTCC+AGG | + | chr4.4:3562471-3562490 | None:intergenic | 45.0% | |
| ACCGAAAACTAGAGCTTGAC+CGG | + | chr4.4:3562427-3562446 | None:intergenic | 45.0% | |
| CAACATGGTTGTTGAGAGGT+TGG | - | chr4.4:3562479-3562498 | MS.gene20134:CDS | 45.0% | |
| CCATGTATTTGACTCCTGCA+AGG | + | chr4.4:3562076-3562095 | None:intergenic | 45.0% | |
| CCTTGCAGGAGTCAAATACA+TGG | - | chr4.4:3562073-3562092 | MS.gene20134:intron | 45.0% | |
| GGGAGATTACCTCATTGATC+AGG | - | chr4.4:3562500-3562519 | MS.gene20134:CDS | 45.0% | |
| GTTTGAGTACCCTCGTCTTA+TGG | - | chr4.4:3562243-3562262 | MS.gene20134:intron | 45.0% | |
| !!! | ACCGGTCAAGCTCTAGTTTT+CGG | - | chr4.4:3562423-3562442 | MS.gene20134:CDS | 45.0% |
| ACCTGGTCATCTTGCTCCTA+CGG | - | chr4.4:3562043-3562062 | MS.gene20134:intron | 50.0% | |
| AGAGCCATCATGGCCAATGA+TGG | + | chr4.4:3561419-3561438 | None:intergenic | 50.0% | |
| ATCATGGCCAATGATGGCAG+CGG | + | chr4.4:3561413-3561432 | None:intergenic | 50.0% | |
| ATGGTCATCCAAGGAGAGTC+TGG | - | chr4.4:3562092-3562111 | MS.gene20134:intron | 50.0% | |
| GACTCCTGGACAATGCAACA+TGG | - | chr4.4:3562464-3562483 | MS.gene20134:CDS | 50.0% | |
| TATGAGGAACCTGTGACTCC+TGG | - | chr4.4:3562450-3562469 | MS.gene20134:CDS | 50.0% | |
| TTGCATTGTCCAGGAGTCAC+AGG | + | chr4.4:3562462-3562481 | None:intergenic | 50.0% | |
| ! | TGGCCATGATGGCTCTGTTT+GGG | - | chr4.4:3561423-3561442 | MS.gene20134:CDS | 50.0% |
| ! | TTGGCCATGATGGCTCTGTT+TGG | - | chr4.4:3561422-3561441 | MS.gene20134:CDS | 50.0% |
| AGCTGTCATCCGTGGGAAGA+AGG | - | chr4.4:3562115-3562134 | MS.gene20134:intron | 55.0% | |
| CAATGATGGCAGCGGCAGAT+AGG | + | chr4.4:3561405-3561424 | None:intergenic | 55.0% | |
| CCTGGTCATCTTGCTCCTAC+GGG | - | chr4.4:3562044-3562063 | MS.gene20134:intron | 55.0% | |
| CTATCTGCCGCTGCCATCAT+TGG | - | chr4.4:3561403-3561422 | MS.gene20134:CDS | 55.0% | |
| GAGCCCAAACAGAGCCATCA+TGG | + | chr4.4:3561429-3561448 | None:intergenic | 55.0% | |
| GCTGCCATCATTGGCCATGA+TGG | - | chr4.4:3561412-3561431 | MS.gene20134:CDS | 55.0% | |
| TGACAGCTCCAGACTCTCCT+TGG | + | chr4.4:3562103-3562122 | None:intergenic | 55.0% | |
| ! | AGTCTGGAGCTGTCATCCGT+GGG | - | chr4.4:3562108-3562127 | MS.gene20134:intron | 55.0% |
| AGCGGCAGATAGGTGGTGTC+CGG | + | chr4.4:3561395-3561414 | None:intergenic | 60.0% | |
| CCCGTAGGAGCAAGATGACC+AGG | + | chr4.4:3562047-3562066 | None:intergenic | 60.0% | |
| TGATGGCAGCGGCAGATAGG+TGG | + | chr4.4:3561402-3561421 | None:intergenic | 60.0% | |
| ! | GAGTCTGGAGCTGTCATCCG+TGG | - | chr4.4:3562107-3562126 | MS.gene20134:intron | 60.0% |
| CCTACGGGCTTGCACCTTGC+AGG | - | chr4.4:3562059-3562078 | MS.gene20134:intron | 65.0% | |
| CCTGCAAGGTGCAAGCCCGT+AGG | + | chr4.4:3562062-3562081 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 3561340 | 3562530 | 3561340 | ID=MS.gene20134 |
| chr4.4 | mRNA | 3561340 | 3562530 | 3561340 | ID=MS.gene20134.t1;Parent=MS.gene20134 |
| chr4.4 | exon | 3562402 | 3562530 | 3562402 | ID=MS.gene20134.t1.exon1;Parent=MS.gene20134.t1 |
| chr4.4 | CDS | 3562402 | 3562530 | 3562402 | ID=cds.MS.gene20134.t1;Parent=MS.gene20134.t1 |
| chr4.4 | exon | 3561734 | 3561871 | 3561734 | ID=MS.gene20134.t1.exon2;Parent=MS.gene20134.t1 |
| chr4.4 | CDS | 3561734 | 3561871 | 3561734 | ID=cds.MS.gene20134.t1;Parent=MS.gene20134.t1 |
| chr4.4 | exon | 3561340 | 3561474 | 3561340 | ID=MS.gene20134.t1.exon3;Parent=MS.gene20134.t1 |
| chr4.4 | CDS | 3561340 | 3561474 | 3561340 | ID=cds.MS.gene20134.t1;Parent=MS.gene20134.t1 |
| Gene Sequence |
| Protein sequence |
