Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20133.t1 | AFK40156.1 | 98.5 | 131 | 2 | 0 | 1 | 131 | 1 | 131 | 1.80E-70 | 275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20133.t1 | P49231 | 88.5 | 131 | 15 | 0 | 1 | 131 | 1 | 131 | 1.3e-66 | 253.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20133.t1 | I3SIR4 | 98.5 | 131 | 2 | 0 | 1 | 131 | 1 | 131 | 1.3e-70 | 275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05946 | MS.gene20133 | 0.977033 | 5.61E-143 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015836 | MS.gene20133 | PPI |
| MS.gene20133 | MS.gene049055 | PPI |
| MS.gene20133 | MS.gene049056 | PPI |
| MS.gene049056 | MS.gene20133 | PPI |
| MS.gene20133 | MS.gene86676 | PPI |
| MS.gene20133 | MS.gene065414 | PPI |
| MS.gene20133 | MS.gene05946 | PPI |
| MS.gene20133 | MS.gene015836 | PPI |
| MS.gene20133 | MS.gene033590 | PPI |
| MS.gene20133 | MS.gene05947 | PPI |
| MS.gene20133 | MS.gene015837 | PPI |
| MS.gene20133 | MS.gene024155 | PPI |
| MS.gene20133 | MS.gene20134 | PPI |
| MS.gene20133 | MS.gene55068 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20133.t1 | MTR_8g102330 | 89.474 | 133 | 12 | 1 | 1 | 131 | 1 | 133 | 8.58e-86 | 246 |
| MS.gene20133.t1 | MTR_6g090030 | 78.947 | 133 | 26 | 1 | 1 | 131 | 1 | 133 | 9.62e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20133.t1 | AT2G19760 | 79.389 | 131 | 27 | 0 | 1 | 131 | 1 | 131 | 8.93e-76 | 221 |
| MS.gene20133.t1 | AT5G56600 | 79.389 | 131 | 27 | 0 | 1 | 131 | 1 | 131 | 9.53e-76 | 221 |
| MS.gene20133.t1 | AT4G29350 | 77.863 | 131 | 29 | 0 | 1 | 131 | 1 | 131 | 1.48e-75 | 220 |
| MS.gene20133.t1 | AT2G19770 | 76.119 | 134 | 29 | 1 | 1 | 131 | 1 | 134 | 9.55e-75 | 218 |
| MS.gene20133.t1 | AT4G29340 | 75.373 | 134 | 30 | 1 | 1 | 131 | 1 | 134 | 1.89e-72 | 213 |
| MS.gene20133.t1 | AT5G56600 | 78.161 | 87 | 19 | 0 | 1 | 87 | 1 | 87 | 3.36e-46 | 145 |
Find 39 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGATTATCTTGTTGAAC+AGG | 0.316615 | 4.4:-3557238 | MS.gene20133:CDS |
| TTGATAGCAGTTATCTCTTC+AGG | 0.316863 | 4.4:+3558238 | None:intergenic |
| TATTGGTGGTACTAAATATA+TGG | 0.344442 | 4.4:-3558170 | MS.gene20133:CDS |
| CCAGTTGGAGCAAGAGTTCC+AGG | 0.346308 | 4.4:+3558199 | None:intergenic |
| GCTCCAACTGGATTACATAT+TGG | 0.350525 | 4.4:-3558187 | MS.gene20133:CDS |
| ACCAATATGGCCTTGGTCAT+TGG | 0.371913 | 4.4:-3557315 | MS.gene20133:CDS |
| GCCAATGACCAAGGCCATAT+TGG | 0.385130 | 4.4:+3557314 | None:intergenic |
| CCTGGAACTCTTGCTCCAAC+TGG | 0.397773 | 4.4:-3558199 | MS.gene20133:CDS |
| TCGGTGTAGACGGTAGCGTT+TGG | 0.405021 | 4.4:-3558638 | MS.gene20133:CDS |
| CGGTGTAGACGGTAGCGTTT+GGG | 0.413449 | 4.4:-3558637 | MS.gene20133:CDS |
| TTGCACTGACCGGGAGTCAT+TGG | 0.432121 | 4.4:+3557279 | None:intergenic |
| AACTACCATGTTGCACTGAC+CGG | 0.438554 | 4.4:+3557269 | None:intergenic |
| TTCCCTCGAATGACAGCTCC+AGG | 0.451609 | 4.4:+3558133 | None:intergenic |
| CACCTTCTCTGTGACATTGA+AGG | 0.461970 | 4.4:-3558687 | MS.gene20133:CDS |
| AATAAAGACTTCGATGAGCC+TGG | 0.476770 | 4.4:-3558217 | MS.gene20133:CDS |
| ATTATATTATCCCAGGGTGC+TGG | 0.481662 | 4.4:-3557357 | MS.gene20133:intron |
| ACTAAATATATGGTGATTCA+AGG | 0.491883 | 4.4:-3558160 | MS.gene20133:CDS |
| TACTGTTAAGAAGACCAATA+TGG | 0.497722 | 4.4:-3557328 | MS.gene20133:CDS |
| TAAGAAGACCAATATGGCCT+TGG | 0.505382 | 4.4:-3557322 | MS.gene20133:CDS |
| CTCACTCATGCTGCTATCCT+CGG | 0.510155 | 4.4:-3558657 | MS.gene20133:CDS |
| ATGGTGATTCAAGGTGAACC+TGG | 0.519803 | 4.4:-3558151 | MS.gene20133:CDS |
| GAACCTGGAGCTGTCATTCG+AGG | 0.527391 | 4.4:-3558136 | MS.gene20133:CDS |
| AGCTGTCATTCGAGGGAAGA+AGG | 0.545445 | 4.4:-3558128 | MS.gene20133:intron |
| GCTGCTATCCTCGGTGTAGA+CGG | 0.548044 | 4.4:-3558648 | MS.gene20133:CDS |
| TCAGAGTGCTAATTTCCCTC+AGG | 0.551578 | 4.4:-3558613 | MS.gene20133:intron |
| ACTACCATGTTGCACTGACC+GGG | 0.553161 | 4.4:+3557270 | None:intergenic |
| ATATTATCCCAGGGTGCTGG+TGG | 0.563632 | 4.4:-3557354 | MS.gene20133:intron |
| CCAACTGGATTACATATTGG+TGG | 0.577382 | 4.4:-3558184 | MS.gene20133:CDS |
| GGTGATTATCTTGTTGAACA+GGG | 0.591801 | 4.4:-3557237 | MS.gene20133:CDS |
| TACCTTCAATGTCACAGAGA+AGG | 0.600366 | 4.4:+3558685 | None:intergenic |
| ATCATAAATGCCAATGACCA+AGG | 0.602159 | 4.4:+3557305 | None:intergenic |
| TATGATGAACCAATGACTCC+CGG | 0.606226 | 4.4:-3557288 | MS.gene20133:CDS |
| CCACCAATATGTAATCCAGT+TGG | 0.607406 | 4.4:+3558184 | None:intergenic |
| AACCTGGAGCTGTCATTCGA+GGG | 0.609180 | 4.4:-3558135 | MS.gene20133:CDS |
| AGTGCAACATGGTAGTTGAA+AGG | 0.610554 | 4.4:-3557263 | MS.gene20133:CDS |
| GACTCCCGGTCAGTGCAACA+TGG | 0.634244 | 4.4:-3557274 | MS.gene20133:CDS |
| AACATGGTAGTTGAAAGGCT+TGG | 0.685081 | 4.4:-3557258 | MS.gene20133:CDS |
| AATCTCTGTGAAGCATGTCG+TGG | 0.689357 | 4.4:-3558728 | None:intergenic |
| AAACGCTACCGTCTACACCG+AGG | 0.799540 | 4.4:+3558640 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTGTTAATTAATTAATTAAG+TGG | - | chr4.4:3557565-3557584 | MS.gene20133:intron | 15.0% |
| !!! | AATTTCTGAATACTTATTTT+GGG | - | chr4.4:3558563-3558582 | MS.gene20133:intron | 15.0% |
| !!! | TAATTTCTGAATACTTATTT+TGG | - | chr4.4:3558562-3558581 | MS.gene20133:intron | 15.0% |
| !!! | TATTTTAATCTTTCAAATTG+AGG | - | chr4.4:3558353-3558372 | MS.gene20133:intron | 15.0% |
| !!! | TTTTAATTTGATAGAGAAAA+TGG | + | chr4.4:3558252-3558271 | None:intergenic | 15.0% |
| !! | ATTGAAAATCTATTTGAAAG+TGG | - | chr4.4:3558271-3558290 | MS.gene20133:intron | 20.0% |
| !! | TTGAAAATCTATTTGAAAGT+GGG | - | chr4.4:3558272-3558291 | MS.gene20133:intron | 20.0% |
| !!! | AACAGTTAAATGCTATTTAT+TGG | - | chr4.4:3558417-3558436 | MS.gene20133:intron | 20.0% |
| !!! | AATGCCATTTTACTATTTTA+GGG | - | chr4.4:3558143-3558162 | MS.gene20133:CDS | 20.0% |
| !!! | TGGTCAATTTTTTTATTCAA+TGG | - | chr4.4:3558490-3558509 | MS.gene20133:intron | 20.0% |
| ! | AAACTGCTCTATTTAATTGT+AGG | - | chr4.4:3557943-3557962 | MS.gene20133:intron | 25.0% |
| ! | AATAGCATTTAACTGTTCAA+AGG | + | chr4.4:3558414-3558433 | None:intergenic | 25.0% |
| ! | AATTTCAAAAAGACCAAACA+TGG | + | chr4.4:3557975-3557994 | None:intergenic | 25.0% |
| ! | ACATCCCTAAAATAGTAAAA+TGG | + | chr4.4:3558150-3558169 | None:intergenic | 25.0% |
| ! | ACTAAATATATGGTGATTCA+AGG | - | chr4.4:3557784-3557803 | MS.gene20133:intron | 25.0% |
| ! | ATTGATCTGAATAAGTCTTA+TGG | - | chr4.4:3558470-3558489 | MS.gene20133:intron | 25.0% |
| ! | CTTATTCAGATCAATATTGA+AGG | + | chr4.4:3558465-3558484 | None:intergenic | 25.0% |
| ! | GAGATTCAATCTAACATAAT+AGG | + | chr4.4:3557922-3557941 | None:intergenic | 25.0% |
| ! | TACAATTTCAGAAATCACAA+TGG | - | chr4.4:3558213-3558232 | MS.gene20133:CDS | 25.0% |
| ! | TTATACATTGTGAAGTGTAA+TGG | - | chr4.4:3557605-3557624 | MS.gene20133:intron | 25.0% |
| !! | GTTCTTTATTTGTAATGTGT+TGG | - | chr4.4:3557658-3557677 | MS.gene20133:intron | 25.0% |
| !! | TAACCTTACACTGATTATTA+AGG | - | chr4.4:3557471-3557490 | MS.gene20133:intron | 25.0% |
| !! | TATTGGTGGTACTAAATATA+TGG | - | chr4.4:3557774-3557793 | MS.gene20133:intron | 25.0% |
| !!! | GAATGCCATTTTACTATTTT+AGG | - | chr4.4:3558142-3558161 | MS.gene20133:CDS | 25.0% |
| !!! | GTTTGGTCTTTTTGAAATTA+AGG | - | chr4.4:3557976-3557995 | MS.gene20133:intron | 25.0% |
| !!! | TTGAAGGTAATTTTTTATGC+AGG | + | chr4.4:3558449-3558468 | None:intergenic | 25.0% |
| AGTCAAATTCTCTGATGAAT+CGG | + | chr4.4:3557531-3557550 | None:intergenic | 30.0% | |
| ATATAAGCAAGTTATTGTGC+AGG | + | chr4.4:3558543-3558562 | None:intergenic | 30.0% | |
| CAAATGAAGTTCTGTACTTA+GGG | - | chr4.4:3557503-3557522 | MS.gene20133:intron | 30.0% | |
| GATCCTTAATAATCAGTGTA+AGG | + | chr4.4:3557477-3557496 | None:intergenic | 30.0% | |
| GTTGTCAATTTAGGAATTTC+AGG | - | chr4.4:3558171-3558190 | MS.gene20133:CDS | 30.0% | |
| TACTGTTAAGAAGACCAATA+TGG | - | chr4.4:3558616-3558635 | MS.gene20133:CDS | 30.0% | |
| !! | CATTTTAGTCACACATTTCA+TGG | + | chr4.4:3558123-3558142 | None:intergenic | 30.0% |
| AGGGATGTTGTTGTCAATTT+AGG | - | chr4.4:3558162-3558181 | MS.gene20133:CDS | 35.0% | |
| ATCATAAATGCCAATGACCA+AGG | + | chr4.4:3558642-3558661 | None:intergenic | 35.0% | |
| ATCTATGCTATGCTATGCTT+TGG | - | chr4.4:3557886-3557905 | MS.gene20133:intron | 35.0% | |
| GCAAATGAAGTTCTGTACTT+AGG | - | chr4.4:3557502-3557521 | MS.gene20133:intron | 35.0% | |
| GGTGATTATCTTGTTGAACA+GGG | - | chr4.4:3558707-3558726 | MS.gene20133:CDS | 35.0% | |
| TGGTGATTATCTTGTTGAAC+AGG | - | chr4.4:3558706-3558725 | MS.gene20133:CDS | 35.0% | |
| TTGGGCATTATATTATCCCA+GGG | - | chr4.4:3558581-3558600 | MS.gene20133:intron | 35.0% | |
| TTTGGGCATTATATTATCCC+AGG | - | chr4.4:3558580-3558599 | MS.gene20133:intron | 35.0% | |
| ! | TTGATAGCAGTTATCTCTTC+AGG | + | chr4.4:3557709-3557728 | None:intergenic | 35.0% |
| AACATGGTAGTTGAAAGGCT+TGG | - | chr4.4:3558686-3558705 | MS.gene20133:CDS | 40.0% | |
| AATAAAGACTTCGATGAGCC+TGG | - | chr4.4:3557727-3557746 | MS.gene20133:intron | 40.0% | |
| AGTGCAACATGGTAGTTGAA+AGG | - | chr4.4:3558681-3558700 | MS.gene20133:CDS | 40.0% | |
| ATTATATTATCCCAGGGTGC+TGG | - | chr4.4:3558587-3558606 | MS.gene20133:intron | 40.0% | |
| CCAACTGGATTACATATTGG+TGG | - | chr4.4:3557760-3557779 | MS.gene20133:intron | 40.0% | |
| CCAATCACAGTAACAGAGAT+CGG | + | chr4.4:3557447-3557466 | None:intergenic | 40.0% | |
| CCACCAATATGTAATCCAGT+TGG | + | chr4.4:3557763-3557782 | None:intergenic | 40.0% | |
| GCTCCAACTGGATTACATAT+TGG | - | chr4.4:3557757-3557776 | MS.gene20133:intron | 40.0% | |
| TAAGAAGACCAATATGGCCT+TGG | - | chr4.4:3558622-3558641 | MS.gene20133:CDS | 40.0% | |
| TACCTTCAATGTCACAGAGA+AGG | + | chr4.4:3557262-3557281 | None:intergenic | 40.0% | |
| TATGATGAACCAATGACTCC+CGG | - | chr4.4:3558656-3558675 | MS.gene20133:CDS | 40.0% | |
| TTGTAGGTTGTTGCCATGTT+TGG | - | chr4.4:3557959-3557978 | MS.gene20133:intron | 40.0% | |
| ! | CACAAGCATTGCCAATGATA+AGG | + | chr4.4:3558304-3558323 | None:intergenic | 40.0% |
| !! | CAGATCGATGGATTGATGAA+AGG | + | chr4.4:3557359-3557378 | None:intergenic | 40.0% |
| !! | GATTGATGAAAGGTACCTGA+GGG | + | chr4.4:3557349-3557368 | None:intergenic | 40.0% |
| ATGGTGATTCAAGGTGAACC+TGG | - | chr4.4:3557793-3557812 | MS.gene20133:intron | 45.0% | |
| CACCTTCTCTGTGACATTGA+AGG | - | chr4.4:3557257-3557276 | MS.gene20133:CDS | 45.0% | |
| CCGATCTCTGTTACTGTGAT+TGG | - | chr4.4:3557444-3557463 | MS.gene20133:intron | 45.0% | |
| TCGCGAAAGAAACAGATCGA+TGG | + | chr4.4:3557371-3557390 | None:intergenic | 45.0% | |
| ! | AACTACCATGTTGCACTGAC+CGG | + | chr4.4:3558678-3558697 | None:intergenic | 45.0% |
| ! | ACCAATATGGCCTTGGTCAT+TGG | - | chr4.4:3558629-3558648 | MS.gene20133:CDS | 45.0% |
| ! | GTGGGTTGCAACCTTATCAT+TGG | - | chr4.4:3558290-3558309 | MS.gene20133:intron | 45.0% |
| ! | TCAGAGTGCTAATTTCCCTC+AGG | - | chr4.4:3557331-3557350 | MS.gene20133:CDS | 45.0% |
| !! | GGATTGATGAAAGGTACCTG+AGG | + | chr4.4:3557350-3557369 | None:intergenic | 45.0% |
| AACCTGGAGCTGTCATTCGA+GGG | - | chr4.4:3557809-3557828 | MS.gene20133:intron | 50.0% | |
| AGCTGTCATTCGAGGGAAGA+AGG | - | chr4.4:3557816-3557835 | MS.gene20133:intron | 50.0% | |
| CTCACTCATGCTGCTATCCT+CGG | - | chr4.4:3557287-3557306 | MS.gene20133:CDS | 50.0% | |
| GCCAATGACCAAGGCCATAT+TGG | + | chr4.4:3558633-3558652 | None:intergenic | 50.0% | |
| ! | ACTACCATGTTGCACTGACC+GGG | + | chr4.4:3558677-3558696 | None:intergenic | 50.0% |
| !! | ATATTATCCCAGGGTGCTGG+TGG | - | chr4.4:3558590-3558609 | MS.gene20133:intron | 50.0% |
| AAACGCTACCGTCTACACCG+AGG | + | chr4.4:3557307-3557326 | None:intergenic | 55.0% | |
| AGTAACACCACCAGCACCCT+GGG | + | chr4.4:3558600-3558619 | None:intergenic | 55.0% | |
| CCAGTTGGAGCAAGAGTTCC+AGG | + | chr4.4:3557748-3557767 | None:intergenic | 55.0% | |
| CCTGGAACTCTTGCTCCAAC+TGG | - | chr4.4:3557745-3557764 | MS.gene20133:intron | 55.0% | |
| CGGTGTAGACGGTAGCGTTT+GGG | - | chr4.4:3557307-3557326 | MS.gene20133:CDS | 55.0% | |
| GAACCTGGAGCTGTCATTCG+AGG | - | chr4.4:3557808-3557827 | MS.gene20133:intron | 55.0% | |
| TCGGTGTAGACGGTAGCGTT+TGG | - | chr4.4:3557306-3557325 | MS.gene20133:CDS | 55.0% | |
| TTCCCTCGAATGACAGCTCC+AGG | + | chr4.4:3557814-3557833 | None:intergenic | 55.0% | |
| ! | GCTGCTATCCTCGGTGTAGA+CGG | - | chr4.4:3557296-3557315 | MS.gene20133:CDS | 55.0% |
| ! | TTGCACTGACCGGGAGTCAT+TGG | + | chr4.4:3558668-3558687 | None:intergenic | 55.0% |
| CAGTAACACCACCAGCACCC+TGG | + | chr4.4:3558601-3558620 | None:intergenic | 60.0% | |
| GACTCCCGGTCAGTGCAACA+TGG | - | chr4.4:3558670-3558689 | MS.gene20133:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 3557230 | 3558736 | 3557230 | ID=MS.gene20133 |
| chr4.4 | mRNA | 3557230 | 3558736 | 3557230 | ID=MS.gene20133.t1;Parent=MS.gene20133 |
| chr4.4 | exon | 3558614 | 3558736 | 3558614 | ID=MS.gene20133.t1.exon1;Parent=MS.gene20133.t1 |
| chr4.4 | CDS | 3558614 | 3558736 | 3558614 | ID=cds.MS.gene20133.t1;Parent=MS.gene20133.t1 |
| chr4.4 | exon | 3558129 | 3558266 | 3558129 | ID=MS.gene20133.t1.exon2;Parent=MS.gene20133.t1 |
| chr4.4 | CDS | 3558129 | 3558266 | 3558129 | ID=cds.MS.gene20133.t1;Parent=MS.gene20133.t1 |
| chr4.4 | exon | 3557230 | 3557364 | 3557230 | ID=MS.gene20133.t1.exon3;Parent=MS.gene20133.t1 |
| chr4.4 | CDS | 3557230 | 3557364 | 3557230 | ID=cds.MS.gene20133.t1;Parent=MS.gene20133.t1 |
| Gene Sequence |
| Protein sequence |
