Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049056.t1 | AFK40156.1 | 98.5 | 131 | 2 | 0 | 1 | 131 | 1 | 131 | 1.80E-70 | 275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049056.t1 | P49231 | 88.5 | 131 | 15 | 0 | 1 | 131 | 1 | 131 | 1.3e-66 | 253.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049056.t1 | I3SIR4 | 98.5 | 131 | 2 | 0 | 1 | 131 | 1 | 131 | 1.3e-70 | 275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene049056 | MS.gene015837 | PPI |
| MS.gene049056 | MS.gene024155 | PPI |
| MS.gene049056 | MS.gene20134 | PPI |
| MS.gene049056 | MS.gene55068 | PPI |
| MS.gene20133 | MS.gene049056 | PPI |
| MS.gene049056 | MS.gene20133 | PPI |
| MS.gene049056 | MS.gene049055 | PPI |
| MS.gene015836 | MS.gene049056 | PPI |
| MS.gene049056 | MS.gene86676 | PPI |
| MS.gene049056 | MS.gene065414 | PPI |
| MS.gene049056 | MS.gene05946 | PPI |
| MS.gene049056 | MS.gene015836 | PPI |
| MS.gene049056 | MS.gene033590 | PPI |
| MS.gene049056 | MS.gene05947 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049056.t1 | MTR_8g102330 | 89.474 | 133 | 12 | 1 | 1 | 131 | 1 | 133 | 8.58e-86 | 246 |
| MS.gene049056.t1 | MTR_6g090030 | 78.947 | 133 | 26 | 1 | 1 | 131 | 1 | 133 | 9.62e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049056.t1 | AT2G19760 | 79.389 | 131 | 27 | 0 | 1 | 131 | 1 | 131 | 8.93e-76 | 221 |
| MS.gene049056.t1 | AT5G56600 | 79.389 | 131 | 27 | 0 | 1 | 131 | 1 | 131 | 9.53e-76 | 221 |
| MS.gene049056.t1 | AT4G29350 | 77.863 | 131 | 29 | 0 | 1 | 131 | 1 | 131 | 1.48e-75 | 220 |
| MS.gene049056.t1 | AT2G19770 | 76.119 | 134 | 29 | 1 | 1 | 131 | 1 | 134 | 9.55e-75 | 218 |
| MS.gene049056.t1 | AT4G29340 | 75.373 | 134 | 30 | 1 | 1 | 131 | 1 | 134 | 1.89e-72 | 213 |
| MS.gene049056.t1 | AT5G56600 | 78.161 | 87 | 19 | 0 | 1 | 87 | 1 | 87 | 3.36e-46 | 145 |
Find 39 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGATTATCTTGTTGAAC+AGG | 0.316615 | 4.2:-2777855 | MS.gene049056:CDS |
| TTGATAGCAGTTATCTCTTC+AGG | 0.316863 | 4.2:+2778860 | None:intergenic |
| TATTGGTGGTACTAAATATA+TGG | 0.344442 | 4.2:-2778792 | MS.gene049056:CDS |
| CCAGTTGGAGCAAGAGTTCC+AGG | 0.346308 | 4.2:+2778821 | None:intergenic |
| GCTCCAACTGGATTACATAT+TGG | 0.350525 | 4.2:-2778809 | MS.gene049056:CDS |
| ACCAATATGGCCTTGGTCAT+TGG | 0.371913 | 4.2:-2777932 | MS.gene049056:CDS |
| GCCAATGACCAAGGCCATAT+TGG | 0.385130 | 4.2:+2777931 | None:intergenic |
| CCTGGAACTCTTGCTCCAAC+TGG | 0.397773 | 4.2:-2778821 | MS.gene049056:CDS |
| TCGGTGTAGACGGTAGCGTT+TGG | 0.405021 | 4.2:-2779260 | MS.gene049056:CDS |
| CGGTGTAGACGGTAGCGTTT+GGG | 0.413449 | 4.2:-2779259 | MS.gene049056:CDS |
| TTGCACTGACCGGGAGTCAT+TGG | 0.432121 | 4.2:+2777896 | None:intergenic |
| AACTACCATGTTGCACTGAC+CGG | 0.438554 | 4.2:+2777886 | None:intergenic |
| TTCCCTCGAATGACAGCTCC+AGG | 0.451609 | 4.2:+2778755 | None:intergenic |
| CACCTTCTCTGTGACATTGA+AGG | 0.461970 | 4.2:-2779309 | MS.gene049056:CDS |
| AATAAAGACTTCGATGAGCC+TGG | 0.476770 | 4.2:-2778839 | MS.gene049056:CDS |
| ATTATATTATCCCAGGGTGC+TGG | 0.481662 | 4.2:-2777974 | MS.gene049056:intron |
| ACTAAATATATGGTGATTCA+AGG | 0.491883 | 4.2:-2778782 | MS.gene049056:CDS |
| TACTGTTAAGAAGACCAATA+TGG | 0.497722 | 4.2:-2777945 | MS.gene049056:CDS |
| TAAGAAGACCAATATGGCCT+TGG | 0.505382 | 4.2:-2777939 | MS.gene049056:CDS |
| CTCACTCATGCTGCTATCCT+CGG | 0.510155 | 4.2:-2779279 | MS.gene049056:CDS |
| ATGGTGATTCAAGGTGAACC+TGG | 0.519803 | 4.2:-2778773 | MS.gene049056:CDS |
| GAACCTGGAGCTGTCATTCG+AGG | 0.527391 | 4.2:-2778758 | MS.gene049056:CDS |
| AGCTGTCATTCGAGGGAAGA+AGG | 0.545445 | 4.2:-2778750 | MS.gene049056:intron |
| GCTGCTATCCTCGGTGTAGA+CGG | 0.548044 | 4.2:-2779270 | MS.gene049056:CDS |
| TCAGAGTGCTAATTTCCCTC+AGG | 0.551578 | 4.2:-2779235 | MS.gene049056:intron |
| ACTACCATGTTGCACTGACC+GGG | 0.553161 | 4.2:+2777887 | None:intergenic |
| ATATTATCCCAGGGTGCTGG+TGG | 0.563632 | 4.2:-2777971 | MS.gene049056:intron |
| CCAACTGGATTACATATTGG+TGG | 0.577382 | 4.2:-2778806 | MS.gene049056:CDS |
| GGTGATTATCTTGTTGAACA+GGG | 0.591801 | 4.2:-2777854 | MS.gene049056:CDS |
| TACCTTCAATGTCACAGAGA+AGG | 0.600366 | 4.2:+2779307 | None:intergenic |
| ATCATAAATGCCAATGACCA+AGG | 0.602159 | 4.2:+2777922 | None:intergenic |
| TATGATGAACCAATGACTCC+CGG | 0.606226 | 4.2:-2777905 | MS.gene049056:CDS |
| CCACCAATATGTAATCCAGT+TGG | 0.607406 | 4.2:+2778806 | None:intergenic |
| AACCTGGAGCTGTCATTCGA+GGG | 0.609180 | 4.2:-2778757 | MS.gene049056:CDS |
| AGTGCAACATGGTAGTTGAA+AGG | 0.610554 | 4.2:-2777880 | MS.gene049056:CDS |
| GACTCCCGGTCAGTGCAACA+TGG | 0.634244 | 4.2:-2777891 | MS.gene049056:CDS |
| AACATGGTAGTTGAAAGGCT+TGG | 0.685081 | 4.2:-2777875 | MS.gene049056:CDS |
| AATCTCTGTGAAGCATGTCG+TGG | 0.689357 | 4.2:-2779350 | None:intergenic |
| AAACGCTACCGTCTACACCG+AGG | 0.799540 | 4.2:+2779262 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTGTTAATTAATTAATTAAG+TGG | - | chr4.2:2778182-2778201 | MS.gene049056:intron | 15.0% |
| !!! | AATTTCTGAATACTTATTTT+GGG | - | chr4.2:2779185-2779204 | MS.gene049056:intron | 15.0% |
| !!! | TAATTTCTGAATACTTATTT+TGG | - | chr4.2:2779184-2779203 | MS.gene049056:intron | 15.0% |
| !!! | TATTTTAATCTTTCAAATTG+AGG | - | chr4.2:2778973-2778992 | MS.gene049056:intron | 15.0% |
| !!! | TTTTAATTTGATAGAGAAAA+TGG | + | chr4.2:2778872-2778891 | None:intergenic | 15.0% |
| !! | ATTGAATATCTATTTGAAAG+TGG | - | chr4.2:2778891-2778910 | MS.gene049056:intron | 20.0% |
| !! | TTGAATATCTATTTGAAAGT+GGG | - | chr4.2:2778892-2778911 | MS.gene049056:intron | 20.0% |
| !!! | AACAGTTAAATGCTATTTAT+TGG | - | chr4.2:2779037-2779056 | MS.gene049056:intron | 20.0% |
| !!! | AATGCCATTTTACTATTTTA+GGG | - | chr4.2:2778760-2778779 | MS.gene049056:CDS | 20.0% |
| !!! | TGGTCAATTTTTTTATTCAA+TGG | - | chr4.2:2779110-2779129 | MS.gene049056:intron | 20.0% |
| ! | AAACTGCTCTATTTAATTGT+AGG | - | chr4.2:2778560-2778579 | MS.gene049056:intron | 25.0% |
| ! | AATAGCATTTAACTGTTCAA+AGG | + | chr4.2:2779034-2779053 | None:intergenic | 25.0% |
| ! | AATTTCAAAAAGACCAAACA+TGG | + | chr4.2:2778592-2778611 | None:intergenic | 25.0% |
| ! | ACAATTTCAGAAATCACAAT+GGG | - | chr4.2:2778831-2778850 | MS.gene049056:CDS | 25.0% |
| ! | ACATCCCTAAAATAGTAAAA+TGG | + | chr4.2:2778767-2778786 | None:intergenic | 25.0% |
| ! | ACTAAATATATGGTGATTCA+AGG | - | chr4.2:2778401-2778420 | MS.gene049056:intron | 25.0% |
| ! | ATTGATCTGAATAAGTCTTA+TGG | - | chr4.2:2779090-2779109 | MS.gene049056:intron | 25.0% |
| ! | CTTATTCAGATCAATATTGA+AGG | + | chr4.2:2779085-2779104 | None:intergenic | 25.0% |
| ! | GAGATTCAATCTAACATAAT+AGG | + | chr4.2:2778539-2778558 | None:intergenic | 25.0% |
| ! | TACAATTTCAGAAATCACAA+TGG | - | chr4.2:2778830-2778849 | MS.gene049056:CDS | 25.0% |
| ! | TTATACATTGTGAAGTGTAA+TGG | - | chr4.2:2778222-2778241 | MS.gene049056:intron | 25.0% |
| !! | GTTCTTTATTTGTAATGTGT+TGG | - | chr4.2:2778275-2778294 | MS.gene049056:intron | 25.0% |
| !! | TAACCTTACACTGATTATTA+AGG | - | chr4.2:2778088-2778107 | MS.gene049056:intron | 25.0% |
| !! | TATTGGTGGTACTAAATATA+TGG | - | chr4.2:2778391-2778410 | MS.gene049056:intron | 25.0% |
| !!! | GAATGCCATTTTACTATTTT+AGG | - | chr4.2:2778759-2778778 | MS.gene049056:CDS | 25.0% |
| !!! | GTTTGGTCTTTTTGAAATTA+AGG | - | chr4.2:2778593-2778612 | MS.gene049056:intron | 25.0% |
| !!! | TTGAAGGTAATTTTTTATGC+AGG | + | chr4.2:2779069-2779088 | None:intergenic | 25.0% |
| AGTCAAATTCTCTGATGAAT+CGG | + | chr4.2:2778148-2778167 | None:intergenic | 30.0% | |
| ATATAAGCAAGTTATTGTGC+AGG | + | chr4.2:2779165-2779184 | None:intergenic | 30.0% | |
| CAAATGAAGTTCTGTACTTA+GGG | - | chr4.2:2778120-2778139 | MS.gene049056:intron | 30.0% | |
| GATCCTTAATAATCAGTGTA+AGG | + | chr4.2:2778094-2778113 | None:intergenic | 30.0% | |
| GTTGTCAATTTAGGAATTTC+AGG | - | chr4.2:2778788-2778807 | MS.gene049056:CDS | 30.0% | |
| TACTGTTAAGAAGACCAATA+TGG | - | chr4.2:2779238-2779257 | MS.gene049056:CDS | 30.0% | |
| !! | CATTTTAGTCACACATTTCA+TGG | + | chr4.2:2778740-2778759 | None:intergenic | 30.0% |
| AGGGATGTTGTTGTCAATTT+AGG | - | chr4.2:2778779-2778798 | MS.gene049056:CDS | 35.0% | |
| ATCATAAATGCCAATGACCA+AGG | + | chr4.2:2779264-2779283 | None:intergenic | 35.0% | |
| ATCTATGCTATGCTATGCTT+TGG | - | chr4.2:2778503-2778522 | MS.gene049056:intron | 35.0% | |
| GCAAATGAAGTTCTGTACTT+AGG | - | chr4.2:2778119-2778138 | MS.gene049056:intron | 35.0% | |
| GGTGATTATCTTGTTGAACA+GGG | - | chr4.2:2779329-2779348 | MS.gene049056:CDS | 35.0% | |
| TGGTGATTATCTTGTTGAAC+AGG | - | chr4.2:2779328-2779347 | MS.gene049056:CDS | 35.0% | |
| TTGGGCATTATATTATCCCA+GGG | - | chr4.2:2779203-2779222 | MS.gene049056:intron | 35.0% | |
| TTTGGGCATTATATTATCCC+AGG | - | chr4.2:2779202-2779221 | MS.gene049056:intron | 35.0% | |
| ! | TTGATAGCAGTTATCTCTTC+AGG | + | chr4.2:2778326-2778345 | None:intergenic | 35.0% |
| AACATGGTAGTTGAAAGGCT+TGG | - | chr4.2:2779308-2779327 | MS.gene049056:CDS | 40.0% | |
| AATAAAGACTTCGATGAGCC+TGG | - | chr4.2:2778344-2778363 | MS.gene049056:intron | 40.0% | |
| AGTGCAACATGGTAGTTGAA+AGG | - | chr4.2:2779303-2779322 | MS.gene049056:CDS | 40.0% | |
| ATTATATTATCCCAGGGTGC+TGG | - | chr4.2:2779209-2779228 | MS.gene049056:intron | 40.0% | |
| CCAACTGGATTACATATTGG+TGG | - | chr4.2:2778377-2778396 | MS.gene049056:intron | 40.0% | |
| CCAATCACAGTAACAGAGAT+CGG | + | chr4.2:2778064-2778083 | None:intergenic | 40.0% | |
| CCACCAATATGTAATCCAGT+TGG | + | chr4.2:2778380-2778399 | None:intergenic | 40.0% | |
| GCTCCAACTGGATTACATAT+TGG | - | chr4.2:2778374-2778393 | MS.gene049056:intron | 40.0% | |
| TAAGAAGACCAATATGGCCT+TGG | - | chr4.2:2779244-2779263 | MS.gene049056:CDS | 40.0% | |
| TACCTTCAATGTCACAGAGA+AGG | + | chr4.2:2777879-2777898 | None:intergenic | 40.0% | |
| TATGATGAACCAATGACTCC+CGG | - | chr4.2:2779278-2779297 | MS.gene049056:CDS | 40.0% | |
| TTGTAGGTTGTTGCCATGTT+TGG | - | chr4.2:2778576-2778595 | MS.gene049056:intron | 40.0% | |
| ! | CACAAGCATTGCCAATGATA+AGG | + | chr4.2:2778924-2778943 | None:intergenic | 40.0% |
| !! | CAGATCGATGGATTGATGAA+AGG | + | chr4.2:2777976-2777995 | None:intergenic | 40.0% |
| !! | GATTGATGAAAGGTACCTGA+GGG | + | chr4.2:2777966-2777985 | None:intergenic | 40.0% |
| ATGGTGATTCAAGGTGAACC+TGG | - | chr4.2:2778410-2778429 | MS.gene049056:intron | 45.0% | |
| CACCTTCTCTGTGACATTGA+AGG | - | chr4.2:2777874-2777893 | MS.gene049056:CDS | 45.0% | |
| CCGATCTCTGTTACTGTGAT+TGG | - | chr4.2:2778061-2778080 | MS.gene049056:intron | 45.0% | |
| TCGCGAAAGAAACAGATCGA+TGG | + | chr4.2:2777988-2778007 | None:intergenic | 45.0% | |
| ! | AACTACCATGTTGCACTGAC+CGG | + | chr4.2:2779300-2779319 | None:intergenic | 45.0% |
| ! | ACCAATATGGCCTTGGTCAT+TGG | - | chr4.2:2779251-2779270 | MS.gene049056:CDS | 45.0% |
| ! | GTGGGTTGCAACCTTATCAT+TGG | - | chr4.2:2778910-2778929 | MS.gene049056:intron | 45.0% |
| ! | TCAGAGTGCTAATTTCCCTC+AGG | - | chr4.2:2777948-2777967 | MS.gene049056:CDS | 45.0% |
| !! | GGATTGATGAAAGGTACCTG+AGG | + | chr4.2:2777967-2777986 | None:intergenic | 45.0% |
| AACCTGGAGCTGTCATTCGA+GGG | - | chr4.2:2778426-2778445 | MS.gene049056:intron | 50.0% | |
| AGCTGTCATTCGAGGGAAGA+AGG | - | chr4.2:2778433-2778452 | MS.gene049056:intron | 50.0% | |
| CTCACTCATGCTGCTATCCT+CGG | - | chr4.2:2777904-2777923 | MS.gene049056:CDS | 50.0% | |
| GCCAATGACCAAGGCCATAT+TGG | + | chr4.2:2779255-2779274 | None:intergenic | 50.0% | |
| ! | ACTACCATGTTGCACTGACC+GGG | + | chr4.2:2779299-2779318 | None:intergenic | 50.0% |
| !! | ATATTATCCCAGGGTGCTGG+TGG | - | chr4.2:2779212-2779231 | MS.gene049056:intron | 50.0% |
| AAACGCTACCGTCTACACCG+AGG | + | chr4.2:2777924-2777943 | None:intergenic | 55.0% | |
| AGTAACACCACCAGCACCCT+GGG | + | chr4.2:2779222-2779241 | None:intergenic | 55.0% | |
| CCAGTTGGAGCAAGAGTTCC+AGG | + | chr4.2:2778365-2778384 | None:intergenic | 55.0% | |
| CCTGGAACTCTTGCTCCAAC+TGG | - | chr4.2:2778362-2778381 | MS.gene049056:intron | 55.0% | |
| CGGTGTAGACGGTAGCGTTT+GGG | - | chr4.2:2777924-2777943 | MS.gene049056:CDS | 55.0% | |
| GAACCTGGAGCTGTCATTCG+AGG | - | chr4.2:2778425-2778444 | MS.gene049056:intron | 55.0% | |
| TCGGTGTAGACGGTAGCGTT+TGG | - | chr4.2:2777923-2777942 | MS.gene049056:CDS | 55.0% | |
| TTCCCTCGAATGACAGCTCC+AGG | + | chr4.2:2778431-2778450 | None:intergenic | 55.0% | |
| ! | GCTGCTATCCTCGGTGTAGA+CGG | - | chr4.2:2777913-2777932 | MS.gene049056:CDS | 55.0% |
| ! | TTGCACTGACCGGGAGTCAT+TGG | + | chr4.2:2779290-2779309 | None:intergenic | 55.0% |
| CAGTAACACCACCAGCACCC+TGG | + | chr4.2:2779223-2779242 | None:intergenic | 60.0% | |
| GACTCCCGGTCAGTGCAACA+TGG | - | chr4.2:2779292-2779311 | MS.gene049056:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 2777847 | 2779358 | 2777847 | ID=MS.gene049056 |
| chr4.2 | mRNA | 2777847 | 2779358 | 2777847 | ID=MS.gene049056.t1;Parent=MS.gene049056 |
| chr4.2 | exon | 2779236 | 2779358 | 2779236 | ID=MS.gene049056.t1.exon1;Parent=MS.gene049056.t1 |
| chr4.2 | CDS | 2779236 | 2779358 | 2779236 | ID=cds.MS.gene049056.t1;Parent=MS.gene049056.t1 |
| chr4.2 | exon | 2778751 | 2778888 | 2778751 | ID=MS.gene049056.t1.exon2;Parent=MS.gene049056.t1 |
| chr4.2 | CDS | 2778751 | 2778888 | 2778751 | ID=cds.MS.gene049056.t1;Parent=MS.gene049056.t1 |
| chr4.2 | exon | 2777847 | 2777981 | 2777847 | ID=MS.gene049056.t1.exon3;Parent=MS.gene049056.t1 |
| chr4.2 | CDS | 2777847 | 2777981 | 2777847 | ID=cds.MS.gene049056.t1;Parent=MS.gene049056.t1 |
| Gene Sequence |
| Protein sequence |
