Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016240.t1 | XP_013449291.1 | 98.6 | 362 | 5 | 0 | 1 | 362 | 71 | 432 | 6.00E-209 | 736.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016240.t1 | Q9Y4P3 | 29.5 | 325 | 207 | 7 | 7 | 331 | 79 | 381 | 1.4e-33 | 145.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016240.t1 | A0A072U288 | 98.6 | 362 | 5 | 0 | 1 | 362 | 71 | 432 | 4.3e-209 | 736.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene86578 | MS.gene016240 | PPI |
| MS.gene30824 | MS.gene016240 | PPI |
| MS.gene024701 | MS.gene016240 | PPI |
| MS.gene21679 | MS.gene016240 | PPI |
| MS.gene21951 | MS.gene016240 | PPI |
| MS.gene62770 | MS.gene016240 | PPI |
| MS.gene22289 | MS.gene016240 | PPI |
| MS.gene79299 | MS.gene016240 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016240.t1 | MTR_7g074890 | 98.619 | 362 | 5 | 0 | 1 | 362 | 71 | 432 | 0.0 | 739 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016240.t1 | AT4G00090 | 77.285 | 361 | 78 | 1 | 1 | 361 | 69 | 425 | 0.0 | 593 |
| MS.gene016240.t1 | AT4G32990 | 23.592 | 373 | 207 | 16 | 10 | 362 | 12 | 326 | 6.14e-12 | 66.2 |
Find 93 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTCCTGATGGACGAAATT+TGG | 0.134819 | 7.4:-30394664 | MS.gene016240:CDS |
| TTCACCTGATGGAAAGATTT+TGG | 0.177641 | 7.4:-30388443 | MS.gene016240:CDS |
| GGGAACACCTTCAGTGTCTT+TGG | 0.241043 | 7.4:+30388520 | None:intergenic |
| GTGATTATTTGCTCAGAGTT+TGG | 0.280324 | 7.4:+30388997 | None:intergenic |
| CAGGAACGGACATCATTCTT+TGG | 0.298478 | 7.4:-30392576 | MS.gene016240:CDS |
| TGTCGAGACTGGAAAGGTTT+TGG | 0.301458 | 7.4:-30388386 | MS.gene016240:CDS |
| AAAGCAATAATTACTATGTT+TGG | 0.304101 | 7.4:-30393313 | MS.gene016240:CDS |
| AGGACGGTTCCTTAAGAATA+TGG | 0.309668 | 7.4:-30388966 | MS.gene016240:CDS |
| TTCAAATGTTATGCAGCTTA+AGG | 0.316013 | 7.4:-30390777 | MS.gene016240:CDS |
| GGCCATGGTGATGCAGTTAC+AGG | 0.333345 | 7.4:-30394696 | MS.gene016240:CDS |
| ATTGATATTCCATATTCTTA+AGG | 0.334674 | 7.4:+30388957 | None:intergenic |
| GTAGAGTCACGAAGAGGAAT+TGG | 0.340083 | 7.4:+30388499 | None:intergenic |
| CACATGCCCCAAACTCTTCC+CGG | 0.345712 | 7.4:+30392543 | None:intergenic |
| TTTGGAGCGTCCGCTACTTA+TGG | 0.351553 | 7.4:-30393295 | MS.gene016240:CDS |
| TCACTTGCAGGCGACATTAC+AGG | 0.354181 | 7.4:-30388240 | MS.gene016240:intron |
| GACTTATCTTCAGAAACTTT+AGG | 0.358893 | 7.4:+30393394 | None:intergenic |
| TTACCCATAGGAATAGCTTT+AGG | 0.374814 | 7.4:+30388204 | None:intergenic |
| TCAAATGTTATGCAGCTTAA+GGG | 0.416385 | 7.4:-30390776 | MS.gene016240:CDS |
| CGATGTTCCCACTTAATCTC+CGG | 0.421960 | 7.4:+30393352 | None:intergenic |
| CACAGAATTAATTTGCCTCC+TGG | 0.426988 | 7.4:-30393520 | MS.gene016240:CDS |
| TATTCTTAAGGAACCGTCCT+TGG | 0.428813 | 7.4:+30388969 | None:intergenic |
| TCTTGAAGTTCGCTATCATA+TGG | 0.443016 | 7.4:-30388551 | MS.gene016240:intron |
| GATTCTTGGGCATACCATCA+TGG | 0.451369 | 7.4:+30388347 | None:intergenic |
| ATTCTTGGGCATACCATCAT+GGG | 0.451700 | 7.4:+30388348 | None:intergenic |
| GATGGACGAAATTTGGCAAC+AGG | 0.460035 | 7.4:-30394657 | MS.gene016240:intron |
| TATGCAGCTTAAGGGGCATA+AGG | 0.464062 | 7.4:-30390768 | MS.gene016240:intron |
| TGCATCACCATGGCCTTTCA+AGG | 0.464432 | 7.4:+30394704 | None:intergenic |
| GGTTGTTCATTGTACATGTA+TGG | 0.468235 | 7.4:-30393427 | MS.gene016240:CDS |
| ACAAGCAAAGCTTCCTCTCC+CGG | 0.468521 | 7.4:-30393371 | MS.gene016240:CDS |
| GTTGCCAAATTTCGTCCATC+AGG | 0.472098 | 7.4:+30394660 | None:intergenic |
| CGCCTCAGTATTTCACCTGA+TGG | 0.474853 | 7.4:-30388454 | MS.gene016240:CDS |
| GCTACTTATGGCAGTGCTGA+TGG | 0.488490 | 7.4:-30393283 | MS.gene016240:CDS |
| GCAGCCAAAATCTTTCCATC+AGG | 0.491333 | 7.4:+30388439 | None:intergenic |
| TAGAGTCACGAAGAGGAATT+GGG | 0.492229 | 7.4:+30388500 | None:intergenic |
| ATTCCTCTTCGTGACTCTAC+TGG | 0.494301 | 7.4:-30388496 | MS.gene016240:CDS |
| TTGGATTTGAATACCTTGAA+AGG | 0.495211 | 7.4:-30394717 | MS.gene016240:CDS |
| TTCCTCTCCCGGAGATTAAG+TGG | 0.497136 | 7.4:-30393360 | MS.gene016240:CDS |
| ATTGCAGTTGGAGGACCTCC+AGG | 0.502096 | 7.4:+30393502 | None:intergenic |
| GGGGCCTAAAGCTATTCCTA+TGG | 0.502990 | 7.4:-30388208 | MS.gene016240:CDS |
| CAGTGGTTAAGTGTCGAGAC+TGG | 0.507986 | 7.4:-30388397 | MS.gene016240:CDS |
| TTCAAGGTATTCAAATCCAA+TGG | 0.512823 | 7.4:+30394720 | None:intergenic |
| AGCGTTCACTGCAGACGTTA+AGG | 0.513771 | 7.4:-30392455 | MS.gene016240:intron |
| GGTGAGAGTATTCAAGTTGG+AGG | 0.518079 | 7.4:-30393667 | MS.gene016240:CDS |
| TTGTTCAGAGTGCTGTGACT+TGG | 0.519779 | 7.4:-30389032 | MS.gene016240:intron |
| CGACATTACAGGCATTGCAT+GGG | 0.526095 | 7.4:-30388229 | MS.gene016240:CDS |
| TCAGCAAATGCAATTGCAGT+TGG | 0.528703 | 7.4:+30393490 | None:intergenic |
| GATGTTCCCACTTAATCTCC+GGG | 0.529101 | 7.4:+30393353 | None:intergenic |
| GCGACATTACAGGCATTGCA+TGG | 0.531987 | 7.4:-30388230 | MS.gene016240:CDS |
| TTGAATACCTTGAAAGGCCA+TGG | 0.533959 | 7.4:-30394711 | MS.gene016240:CDS |
| AGTCAAGCGGCATCATCCAT+TGG | 0.534347 | 7.4:-30394736 | MS.gene016240:CDS |
| CGGGAAGAGTTTGGGGCATG+TGG | 0.534451 | 7.4:-30392542 | MS.gene016240:CDS |
| GTGCAAACAGCTTGTGCTGA+TGG | 0.534976 | 7.4:-30393693 | MS.gene016240:intron |
| ACGGACATCATTCTTTGGCA+TGG | 0.536612 | 7.4:-30392571 | MS.gene016240:CDS |
| GGGATATGTTTCTCTCCTGA+TGG | 0.536826 | 7.4:-30394675 | MS.gene016240:CDS |
| TTAAGAATATGGAATATCAA+TGG | 0.537043 | 7.4:-30388955 | MS.gene016240:intron |
| AGCTGCAATGAAACGTCCAT+TGG | 0.543756 | 7.4:+30392480 | None:intergenic |
| CAAATGTTATGCAGCTTAAG+GGG | 0.548411 | 7.4:-30390775 | MS.gene016240:CDS |
| GCAAATGCAATTGCAGTTGG+AGG | 0.553636 | 7.4:+30393493 | None:intergenic |
| CTGGGAGATTGTATATTCAA+AGG | 0.557654 | 7.4:-30390819 | MS.gene016240:CDS |
| AGTGGTGAGAGTATTCAAGT+TGG | 0.558329 | 7.4:-30393670 | MS.gene016240:CDS |
| GTGACAAGAAAGTGAAATTG+TGG | 0.564897 | 7.4:-30387775 | MS.gene016240:CDS |
| GACATTACAGGCATTGCATG+GGG | 0.566726 | 7.4:-30388228 | MS.gene016240:CDS |
| ACCACTGCAATGTGGAACCA+TGG | 0.567269 | 7.4:+30388413 | None:intergenic |
| TCCCACTTAATCTCCGGGAG+AGG | 0.568667 | 7.4:+30393358 | None:intergenic |
| GCTGCAATGAAACGTCCATT+GGG | 0.569996 | 7.4:+30392481 | None:intergenic |
| GAGATTGTATATTCAAAGGA+TGG | 0.573331 | 7.4:-30390815 | MS.gene016240:CDS |
| TGACAAGAAAGTGAAATTGT+GGG | 0.573767 | 7.4:-30387774 | MS.gene016240:CDS |
| GCAAATAATCACTGCATCCA+AGG | 0.580008 | 7.4:-30388986 | MS.gene016240:CDS |
| TTAATTGCATCGTGTTCAGA+AGG | 0.582676 | 7.4:-30393253 | MS.gene016240:intron |
| TCCTCTCCCGGAGATTAAGT+GGG | 0.590082 | 7.4:-30393359 | MS.gene016240:CDS |
| GGGCCTAAAGCTATTCCTAT+GGG | 0.601225 | 7.4:-30388207 | MS.gene016240:intron |
| CCACTGCAATGTGGAACCAT+GGG | 0.607190 | 7.4:+30388414 | None:intergenic |
| GCCATGGTGATGCAGTTACA+GGG | 0.611622 | 7.4:-30394695 | MS.gene016240:CDS |
| GTTAAGTGTCGAGACTGGAA+AGG | 0.613018 | 7.4:-30388392 | MS.gene016240:CDS |
| AGTTCGCTATCATATGGATG+AGG | 0.619477 | 7.4:-30388545 | MS.gene016240:CDS |
| GTGTCATCACACAATCTCAC+TGG | 0.620767 | 7.4:-30393448 | MS.gene016240:CDS |
| ATCAGCACTGCCATAAGTAG+CGG | 0.630073 | 7.4:+30393285 | None:intergenic |
| CCCATGGTTCCACATTGCAG+TGG | 0.633715 | 7.4:-30388414 | MS.gene016240:CDS |
| ATGGCTACTATATCACCCAA+TGG | 0.633994 | 7.4:-30392496 | MS.gene016240:CDS |
| ATAATCACTGCATCCAAGGA+CGG | 0.635724 | 7.4:-30388982 | MS.gene016240:CDS |
| AACAGCTTGTGCTGATGGAG+TGG | 0.638047 | 7.4:-30393688 | MS.gene016240:intron |
| AGAATTAATTTGCCTCCTGG+AGG | 0.640059 | 7.4:-30393517 | MS.gene016240:CDS |
| TGAGGATCCAAAGACACTGA+AGG | 0.648025 | 7.4:-30388527 | MS.gene016240:CDS |
| GACACTTAACCACTGCAATG+TGG | 0.660431 | 7.4:+30388405 | None:intergenic |
| GTACCAGTAGAGTCACGAAG+AGG | 0.665126 | 7.4:+30388493 | None:intergenic |
| AACATAAGGATCAAGTCAAG+CGG | 0.669504 | 7.4:-30394749 | None:intergenic |
| TTCCATCAGGTGAAATACTG+AGG | 0.672850 | 7.4:+30388452 | None:intergenic |
| TCCCTGTAACTGCATCACCA+TGG | 0.687110 | 7.4:+30394694 | None:intergenic |
| ACATTACAGGCATTGCATGG+GGG | 0.704948 | 7.4:-30388227 | MS.gene016240:CDS |
| ATTGTGTGATGACACAACAA+TGG | 0.716207 | 7.4:+30393456 | None:intergenic |
| GCAGTTGGAGGACCTCCAGG+AGG | 0.740102 | 7.4:+30393505 | None:intergenic |
| GGTGCACTAGTAGTTCACGA+TGG | 0.754480 | 7.4:+30387746 | None:intergenic |
| GTGTGATGACACAACAATGG+AGG | 0.768302 | 7.4:+30393459 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 30387760 | 30394763 | 30387760 | ID=MS.gene016240 |
| chr7.4 | mRNA | 30387760 | 30394763 | 30387760 | ID=MS.gene016240.t1;Parent=MS.gene016240 |
| chr7.4 | exon | 30394658 | 30394763 | 30394658 | ID=MS.gene016240.t1.exon1;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30394658 | 30394763 | 30394658 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| chr7.4 | exon | 30393645 | 30393705 | 30393645 | ID=MS.gene016240.t1.exon2;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30393645 | 30393705 | 30393645 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| chr7.4 | exon | 30393254 | 30393546 | 30393254 | ID=MS.gene016240.t1.exon3;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30393254 | 30393546 | 30393254 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| chr7.4 | exon | 30392456 | 30392595 | 30392456 | ID=MS.gene016240.t1.exon4;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30392456 | 30392595 | 30392456 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| chr7.4 | exon | 30390769 | 30390843 | 30390769 | ID=MS.gene016240.t1.exon5;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30390769 | 30390843 | 30390769 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| chr7.4 | exon | 30388956 | 30389046 | 30388956 | ID=MS.gene016240.t1.exon6;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30388956 | 30389046 | 30388956 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| chr7.4 | exon | 30388362 | 30388565 | 30388362 | ID=MS.gene016240.t1.exon7;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30388362 | 30388565 | 30388362 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| chr7.4 | exon | 30388208 | 30388252 | 30388208 | ID=MS.gene016240.t1.exon8;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30388208 | 30388252 | 30388208 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| chr7.4 | exon | 30387760 | 30387833 | 30387760 | ID=MS.gene016240.t1.exon9;Parent=MS.gene016240.t1 |
| chr7.4 | CDS | 30387760 | 30387833 | 30387760 | ID=cds.MS.gene016240.t1;Parent=MS.gene016240.t1 |
| Gene Sequence |
| Protein sequence |
