AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016246

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016246.t1 MTR_7g074850 92.239 335 18 3 1 333 1 329 0.0 632
MS.gene016246.t1 MTR_7g074820 86.866 335 36 3 1 333 1 329 0.0 587
MS.gene016246.t1 MTR_7g074730 82.985 335 48 4 1 333 1 328 0.0 564
MS.gene016246.t1 MTR_7g074880 73.731 335 77 4 1 333 1 326 0.0 511
MS.gene016246.t1 MTR_7g074710 65.075 335 106 4 1 333 1 326 4.11e-161 453
MS.gene016246.t1 MTR_7g074870 70.307 293 77 3 43 333 4 288 2.76e-149 421
MS.gene016246.t1 MTR_4g023730 50.595 336 156 4 1 333 1 329 4.95e-108 318
MS.gene016246.t1 MTR_1g022440 46.483 327 165 4 8 333 8 325 2.33e-100 299
MS.gene016246.t1 MTR_1g022445 46.483 327 165 5 8 333 8 325 1.90e-96 289
MS.gene016246.t1 MTR_4g092080 39.941 338 188 4 1 331 7 336 1.55e-78 243
MS.gene016246.t1 MTR_4g081440 36.994 346 186 10 6 333 18 349 5.73e-70 222
MS.gene016246.t1 MTR_4g081440 36.994 346 187 10 6 333 22 354 6.13e-70 222
MS.gene016246.t1 MTR_2g101330 37.273 330 190 9 7 333 4 319 2.74e-63 204
MS.gene016246.t1 MTR_2g013230 35.057 348 203 8 1 333 1 340 4.26e-56 186
MS.gene016246.t1 MTR_3g005210 34.535 333 193 8 8 333 6 320 8.33e-56 184
MS.gene016246.t1 MTR_4g077130 34.808 339 197 11 1 332 1 322 6.53e-55 182
MS.gene016246.t1 MTR_8g062110 35.821 335 193 10 1 330 2 319 2.32e-54 181
MS.gene016246.t1 MTR_8g062110 35.522 335 194 9 1 330 11 328 3.31e-54 181
MS.gene016246.t1 MTR_8g062440 35.503 338 197 9 1 333 1 322 1.07e-53 179
MS.gene016246.t1 MTR_5g056600 35.347 331 192 10 9 333 5 319 1.43e-53 179
MS.gene016246.t1 MTR_3g031650 34.925 335 189 11 8 333 5 319 5.24e-52 174
MS.gene016246.t1 MTR_8g062440 34.911 338 198 9 1 333 1 321 1.03e-51 174
MS.gene016246.t1 MTR_8g062110 36.806 288 164 6 1 285 2 274 3.58e-51 171
MS.gene016246.t1 MTR_8g062110 36.806 288 164 6 1 285 2 274 3.91e-51 171
MS.gene016246.t1 MTR_3g005170 33.939 330 197 10 8 332 10 323 8.22e-51 171
MS.gene016246.t1 MTR_2g013250 33.430 344 206 9 3 331 2 337 5.27e-50 170
MS.gene016246.t1 MTR_3g005170 35.816 282 164 7 8 286 10 277 2.61e-49 166
MS.gene016246.t1 MTR_4g077100 35.172 290 170 7 1 286 3 278 1.21e-48 164
MS.gene016246.t1 MTR_8g062440 37.063 286 162 6 1 283 1 271 1.85e-48 166
MS.gene016246.t1 MTR_5g072620 35.542 332 191 11 8 333 5 319 3.00e-48 165
MS.gene016246.t1 MTR_4g077100 35.517 290 169 8 1 286 3 278 3.12e-48 165
MS.gene016246.t1 MTR_2g013260 34.393 346 204 8 1 331 1 338 1.06e-47 164
MS.gene016246.t1 MTR_3g031650 35.563 284 160 8 8 285 5 271 8.26e-46 157
MS.gene016246.t1 MTR_4g009040 34.545 330 195 10 7 333 12 323 1.49e-45 158
MS.gene016246.t1 MTR_7g117190 33.333 348 206 11 3 333 2 340 2.47e-44 155
MS.gene016246.t1 MTR_4g009040 34.030 335 194 11 7 333 12 327 4.32e-44 154
MS.gene016246.t1 MTR_2g104960 33.636 330 195 12 8 333 20 329 3.47e-43 152
MS.gene016246.t1 MTR_4g006940 32.424 330 199 10 8 333 17 326 3.69e-43 152
MS.gene016246.t1 MTR_2g028620 32.853 347 194 13 2 333 4 326 9.12e-42 148
MS.gene016246.t1 MTR_2g013260 34.333 300 175 7 1 285 1 293 1.20e-39 141
MS.gene016246.t1 MTR_4g006940 34.043 282 165 8 8 287 17 279 4.10e-39 140
MS.gene016246.t1 MTR_4g006940 34.043 282 165 8 8 287 17 279 4.10e-39 140
MS.gene016246.t1 MTR_4g009690 33.030 330 199 10 7 333 12 322 5.76e-39 140
MS.gene016246.t1 MTR_2g028620 34.615 286 164 9 2 280 4 273 1.05e-38 139
MS.gene016246.t1 MTR_4g009690 32.523 329 203 9 7 333 12 323 7.43e-38 137
MS.gene016246.t1 MTR_7g117190 33.445 299 176 9 3 285 2 293 2.99e-36 133
MS.gene016246.t1 MTR_3g005170 30.303 330 178 11 8 332 10 292 3.84e-35 130
MS.gene016246.t1 MTR_2g013230 32.510 243 148 6 106 333 9 250 1.11e-29 114
MS.gene016246.t1 MTR_8g062110 32.000 225 138 7 111 330 19 233 1.02e-25 103
MS.gene016246.t1 MTR_5g029990 28.896 308 179 14 8 305 12 289 9.52e-25 102
MS.gene016246.t1 MTR_8g062110 32.584 178 109 3 111 285 19 188 1.62e-22 93.6
MS.gene016246.t1 MTR_7g117190 32.174 230 137 9 121 333 3 230 5.47e-22 93.2
MS.gene016246.t1 MTR_0164s0070 37.313 134 76 3 7 139 4 130 2.33e-21 90.9
MS.gene016246.t1 MTR_2g013260 31.349 252 153 7 95 331 21 267 5.67e-21 91.3
MS.gene016246.t1 MTR_6g406250 28.571 266 165 10 5 262 52 300 8.15e-21 92.0
MS.gene016246.t1 MTR_7g074865 85.366 41 6 0 1 41 1 41 3.48e-19 80.9
MS.gene016246.t1 MTR_6g406250 27.511 229 144 8 2 222 47 261 1.64e-17 81.3
MS.gene016246.t1 MTR_3g101500 27.338 278 162 8 7 277 3 247 5.91e-14 71.6
MS.gene016246.t1 MTR_3g101500 26.568 271 173 6 7 277 3 247 8.73e-14 71.6
MS.gene016246.t1 MTR_0685s0030 31.624 117 76 1 170 286 3 115 1.22e-13 67.0
MS.gene016246.t1 MTR_0685s0030 31.624 117 76 1 170 286 3 115 3.34e-13 67.0
MS.gene016246.t1 MTR_3g101500 29.949 197 112 6 7 203 3 173 1.19e-12 67.0
MS.gene016246.t1 MTR_3g011130 33.913 115 69 3 111 221 35 146 8.76e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016246.t1 AT2G45400 49.263 339 155 6 3 333 35 364 3.49e-104 310
MS.gene016246.t1 AT5G42800 47.164 335 165 6 1 333 1 325 2.89e-96 290
MS.gene016246.t1 AT2G45400 50.169 295 135 4 3 292 35 322 2.29e-93 281
MS.gene016246.t1 AT2G45400 48.322 298 141 5 42 333 17 307 8.93e-85 258
MS.gene016246.t1 AT4G35420 41.888 339 173 10 1 333 1 321 2.79e-80 248
MS.gene016246.t1 AT1G61720 38.576 337 186 8 7 333 12 337 1.09e-71 226
MS.gene016246.t1 AT2G45400 52.444 225 97 3 3 222 35 254 1.28e-71 223
MS.gene016246.t1 AT1G68540 38.906 329 183 10 10 333 6 321 6.56e-58 190
MS.gene016246.t1 AT1G09480 36.667 330 190 8 8 333 8 322 1.93e-56 186
MS.gene016246.t1 AT5G58490 37.537 341 186 12 1 333 2 323 3.09e-56 186
MS.gene016246.t1 AT2G02400 36.119 335 189 10 5 333 3 318 3.36e-56 185
MS.gene016246.t1 AT1G09480 36.667 330 190 8 8 333 55 369 4.41e-56 186
MS.gene016246.t1 AT1G09490 35.455 330 194 8 8 333 8 322 7.49e-55 182
MS.gene016246.t1 AT1G51410 34.226 336 200 8 3 333 4 323 8.57e-55 182
MS.gene016246.t1 AT5G19440 36.061 330 190 9 8 332 10 323 9.18e-55 182
MS.gene016246.t1 AT4G27250 32.749 342 202 8 2 325 7 338 9.53e-55 182
MS.gene016246.t1 AT1G25460 36.280 328 191 8 10 333 6 319 5.08e-53 177
MS.gene016246.t1 AT1G09510 36.474 329 186 9 8 330 8 319 6.32e-53 177
MS.gene016246.t1 AT4G27250 31.751 337 202 7 2 325 7 328 1.27e-52 177
MS.gene016246.t1 AT1G66800 34.132 334 203 8 1 333 1 318 2.24e-52 176
MS.gene016246.t1 AT1G76470 35.714 322 193 6 5 324 4 313 1.36e-49 168
MS.gene016246.t1 AT1G09500 35.357 280 163 6 8 283 8 273 2.56e-46 159
MS.gene016246.t1 AT1G09500 35.294 289 169 7 8 292 8 282 3.93e-46 159
MS.gene016246.t1 AT1G15950 34.848 330 191 10 8 333 13 322 6.91e-46 159
MS.gene016246.t1 AT1G15950 34.848 330 191 10 8 333 13 322 6.91e-46 159
MS.gene016246.t1 AT2G33590 34.139 331 184 12 5 325 6 312 2.13e-43 152
MS.gene016246.t1 AT1G15950 34.277 318 186 8 8 321 13 311 2.31e-43 152
MS.gene016246.t1 AT1G80820 34.835 333 187 11 8 333 8 317 5.69e-42 149
MS.gene016246.t1 AT1G15950 36.879 282 157 8 8 287 13 275 3.00e-41 145
MS.gene016246.t1 AT1G15950 36.749 283 158 8 8 288 13 276 3.16e-41 145
MS.gene016246.t1 AT1G15950 36.396 283 159 7 8 288 13 276 3.85e-41 145
MS.gene016246.t1 AT2G33600 35.258 329 185 12 4 325 5 312 2.57e-40 144
MS.gene016246.t1 AT1G09490 33.096 281 176 6 57 333 19 291 2.66e-40 143
MS.gene016246.t1 AT1G80820 35.789 285 156 9 8 287 8 270 1.05e-37 136
MS.gene016246.t1 AT1G68540 36.364 220 130 6 118 333 1 214 3.90e-33 122
MS.gene016246.t1 AT1G09500 32.917 240 150 5 57 292 16 248 3.89e-32 122
MS.gene016246.t1 AT2G23910 26.183 317 194 12 9 310 11 302 4.10e-25 103
MS.gene016246.t1 AT2G23910 26.756 299 183 10 1 288 1 274 1.48e-23 98.6
MS.gene016246.t1 AT2G23910 25.926 297 184 11 1 286 1 272 2.23e-23 97.8
MS.gene016246.t1 AT1G15950 31.718 227 143 7 111 333 6 224 1.13e-22 95.5
MS.gene016246.t1 AT5G14700 29.889 271 154 9 8 262 55 305 2.12e-22 96.7
MS.gene016246.t1 AT4G30470 24.825 286 190 9 9 290 11 275 3.54e-21 92.4
MS.gene016246.t1 AT5G14700 28.390 236 139 7 8 230 55 273 1.24e-18 85.1
MS.gene016246.t1 AT4G33360 25.606 289 185 6 2 286 9 271 1.13e-17 82.4
MS.gene016246.t1 AT2G23910 25.670 261 165 10 63 310 24 268 1.38e-17 81.6
MS.gene016246.t1 AT4G33360 25.606 289 185 6 2 286 9 271 1.75e-17 82.4
MS.gene016246.t1 AT4G33360 25.352 284 182 6 7 286 2 259 6.94e-17 80.5

Find 66 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAATCTGTTCTTGAATT+TGG 0.142678 7.1:-28201100 MS.gene016246:CDS
AGTTTACTAGCTTTCTTATT+TGG 0.183198 7.1:-28200989 MS.gene016246:intron
CATTGAAAATATTTAGCTTT+TGG 0.214602 7.1:+28201446 None:intergenic
GAGCAATTATATCTCCCTTT+TGG 0.266827 7.1:+28200774 None:intergenic
ACTGGAGGTACAGGGTTTAT+TGG 0.300052 7.1:-28203515 MS.gene016246:CDS
ATACAATGGATGAAAGTTAT+TGG 0.306077 7.1:-28201192 MS.gene016246:CDS
AGAACCATTGATGGAGCATT+AGG 0.319772 7.1:-28201307 MS.gene016246:CDS
GATGTTGGCATTTGAAGTTC+AGG 0.343651 7.1:+28200699 None:intergenic
CATTTGAAGTTCAGGGTACT+TGG 0.365683 7.1:+28200707 None:intergenic
AATTGAATGTTGCAAGGAAA+AGG 0.371801 7.1:-28200459 MS.gene016246:CDS
AGCATCCATGAGTTTCTTAG+AGG 0.383422 7.1:+28200526 None:intergenic
AAAGGAAGAGTTTGTGTGAC+TGG 0.394367 7.1:-28203533 MS.gene016246:CDS
GTACAGGGTTTATTGGTTCA+TGG 0.397157 7.1:-28203508 MS.gene016246:CDS
ATTGCTGCATTAAAACTCTC+AGG 0.400078 7.1:+28201409 None:intergenic
GCATTTGGAAGGGCTGCATT+TGG 0.401537 7.1:+28201472 None:intergenic
TCTTTCAGTTCAAAATCAAC+TGG 0.415670 7.1:+28201352 None:intergenic
GAGGTTAATTGCGGAAGCTT+AGG 0.430315 7.1:+28200545 None:intergenic
GGTACGAGATACAAGCAATA+AGG 0.431637 7.1:+28200857 None:intergenic
TCTTCAATAGTTGCAATGAA+TGG 0.434791 7.1:+28200750 None:intergenic
CTTGAACATCCTAATCCAAA+AGG 0.439060 7.1:-28200789 MS.gene016246:CDS
GTTTGTGTGACTGGAGGTAC+AGG 0.443705 7.1:-28203524 MS.gene016246:CDS
TCATCTTCTAGAGAGCTTAT+CGG 0.460094 7.1:-28200572 MS.gene016246:intron
AATTCCTAATGCTCCATCAA+TGG 0.462695 7.1:+28201303 None:intergenic
ATTAAGAGACTCCTTGAAGA+TGG 0.464111 7.1:-28203482 MS.gene016246:CDS
TTCATTTGTCCTAAGCTACC+TGG 0.469552 7.1:-28201022 MS.gene016246:CDS
TAACAGCGTAACCATCTTCA+AGG 0.473218 7.1:+28203471 None:intergenic
ATGAAAGGTCCTACAACAAA+AGG 0.476037 7.1:+28201040 None:intergenic
TAGCCTTGACGAAATGTTTG+AGG 0.478982 7.1:-28200486 MS.gene016246:CDS
ATTTGGAGAACAACATGGGT+TGG 0.487832 7.1:-28201083 MS.gene016246:CDS
CTTGAATTTGGAGAACAACA+TGG 0.492029 7.1:-28201088 MS.gene016246:CDS
ATTGAATGTTGCAAGGAAAA+GGG 0.495429 7.1:-28200458 MS.gene016246:CDS
AAGAACAGATTTCTCAGCTA+GGG 0.495734 7.1:+28201108 None:intergenic
TCTAAGAAACTCATGGATGC+TGG 0.498821 7.1:-28200524 MS.gene016246:CDS
TTATTGCTTGTATCTCGTAC+CGG 0.499138 7.1:-28200855 MS.gene016246:CDS
GCAGCAATTGAAGGGTGCAT+TGG 0.503156 7.1:-28201394 MS.gene016246:CDS
TGAACAGATCCAGGTAGCTT+AGG 0.503818 7.1:+28201013 None:intergenic
CAAGAACAGATTTCTCAGCT+AGG 0.509193 7.1:+28201107 None:intergenic
ATGTTGGCATTTGAAGTTCA+GGG 0.512887 7.1:+28200700 None:intergenic
GTAGTAATGGAAGAAGAGAA+AGG 0.514620 7.1:-28203551 None:intergenic
ATTAACCTCTAAGAAACTCA+TGG 0.523086 7.1:-28200531 MS.gene016246:CDS
ACACCATACGACCAAGCAAA+AGG 0.525769 7.1:+28201139 None:intergenic
GCTTGTATCTCGTACCGGTA+TGG 0.528101 7.1:-28200850 MS.gene016246:CDS
AGTAAACTATGAACAGATCC+AGG 0.532869 7.1:+28201004 None:intergenic
GCATGTTGATGATGTGGCTA+GGG 0.545455 7.1:-28200826 MS.gene016246:CDS
TGTAGAACTCACTTGGATGT+TGG 0.557000 7.1:+28200684 None:intergenic
ATCATCAACATGCACCATAC+CGG 0.559899 7.1:+28200836 None:intergenic
ACCACTGTTAGAGCTGATCC+AGG 0.562994 7.1:-28203446 MS.gene016246:intron
TTGAACATCCTAATCCAAAA+GGG 0.565903 7.1:-28200788 MS.gene016246:CDS
TTGAATTTGGAGAACAACAT+GGG 0.580887 7.1:-28201087 MS.gene016246:CDS
AGACAAAGAAGAAGATACAA+TGG 0.599895 7.1:-28201206 MS.gene016246:CDS
TTGCTGCATTAAAACTCTCA+GGG 0.608456 7.1:+28201410 None:intergenic
GGACGCAATTGAATGTTGCA+AGG 0.609289 7.1:-28200465 MS.gene016246:CDS
CAGGGTACTTGGAAGAAACA+AGG 0.616116 7.1:+28200718 None:intergenic
CGTCCTCAAACATTTCGTCA+AGG 0.621284 7.1:+28200483 None:intergenic
TTTGTGTGACTGGAGGTACA+GGG 0.633409 7.1:-28203523 MS.gene016246:CDS
ATACACAGCAGAAGCACTTG+AGG 0.642464 7.1:+28201234 None:intergenic
TATGGTGCATGTTGATGATG+TGG 0.643007 7.1:-28200832 MS.gene016246:CDS
AGTTTCTTAGAGGTTAATTG+CGG 0.647629 7.1:+28200536 None:intergenic
TGCATGTTGATGATGTGGCT+AGG 0.650912 7.1:-28200827 MS.gene016246:CDS
AAAGGTCCTACAACAAAAGG+TGG 0.652252 7.1:+28201043 None:intergenic
CTGCATTTGGAAGATTTGCG+AGG 0.654312 7.1:+28201485 None:intergenic
ACCTGGATCAGCTCTAACAG+TGG 0.657128 7.1:+28203445 None:intergenic
AATCAACTGGAGTAGCTGTG+TGG 0.675600 7.1:+28201365 None:intergenic
TCTTCTAGAGAGCTTATCGG+AGG 0.675738 7.1:-28200569 MS.gene016246:intron
GGTAGCTTAGGACAAATGAA+AGG 0.676303 7.1:+28201025 None:intergenic
GGAAGAGTTTGTGTGACTGG+AGG 0.702220 7.1:-28203530 MS.gene016246:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAATTAATAATATAT+AGG + chr7.1:28201930-28201949 None:intergenic 0.0%
!! AAAATAATTTAGAAATTTGA+AGG + chr7.1:28201078-28201097 None:intergenic 10.0%
!! AATTATTAGAAAAATTTGAA+TGG - chr7.1:28201259-28201278 MS.gene016246:CDS 10.0%
!! ATAGATATAAAAAATGTTAT+TGG - chr7.1:28201217-28201236 MS.gene016246:CDS 10.0%
!! TAAATTATAAAAATTTCTCT+TGG - chr7.1:28201114-28201133 MS.gene016246:CDS 10.0%
!!! ATTTAACTTTTTTTTTAAGA+AGG + chr7.1:28200770-28200789 None:intergenic 10.0%
!!! TTATTCTATTTTTTTTATCT+TGG + chr7.1:28200861-28200880 None:intergenic 10.0%
!! AAAAAAAAAGTTAAATTACC+CGG - chr7.1:28200774-28200793 MS.gene016246:CDS 15.0%
!! AATAAACTACATATTGTTAT+TGG - chr7.1:28200604-28200623 MS.gene016246:intron 15.0%
!! AATAGATGAAAATTAACAAA+TGG - chr7.1:28203082-28203101 MS.gene016246:intron 15.0%
!! AATTAATAATATATAGGTAG+TGG + chr7.1:28201924-28201943 None:intergenic 15.0%
!! TAAAAATGAAACACATTTAT+AGG - chr7.1:28201309-28201328 MS.gene016246:CDS 15.0%
!! TTTAAATAAACTAATATGAC+AGG - chr7.1:28201381-28201400 MS.gene016246:CDS 15.0%
!!! AGTTTTTTTTACTTTCAAAA+AGG + chr7.1:28200577-28200596 None:intergenic 15.0%
!!! ATAAGACTTAAATAAGTTTT+TGG - chr7.1:28200895-28200914 MS.gene016246:intron 15.0%
!!! ATTGAAAATATTTAGCTTTT+GGG + chr7.1:28202549-28202568 None:intergenic 15.0%
!!! CTTTAATTTATCTATTTTGA+AGG + chr7.1:28201052-28201071 None:intergenic 15.0%
!!! GTTTTTTTTACTTTCAAAAA+GGG + chr7.1:28200576-28200595 None:intergenic 15.0%
!!! TTAAATTTTTCTATAGTAGA+TGG + chr7.1:28202065-28202084 None:intergenic 15.0%
!!! TTGGAAAACTTTTTTTTTAA+AGG - chr7.1:28201236-28201255 MS.gene016246:CDS 15.0%
!!! TTTTTTATCTCTATTTTTTC+CGG + chr7.1:28200796-28200815 None:intergenic 15.0%
!! AACATTGTATAATTAGATCA+TGG + chr7.1:28201484-28201503 None:intergenic 20.0%
!! AAGATGAAGAAAAATTTAGA+CGG + chr7.1:28200974-28200993 None:intergenic 20.0%
!! ACATTGTATAATTAGATCAT+GGG + chr7.1:28201483-28201502 None:intergenic 20.0%
!! ATTGTTTGAGAAAAAAATGA+GGG - chr7.1:28201284-28201303 MS.gene016246:CDS 20.0%
!! TATTGTTTGAGAAAAAAATG+AGG - chr7.1:28201283-28201302 MS.gene016246:CDS 20.0%
!! TTAAACAACCTAAGAATATA+TGG + chr7.1:28202364-28202383 None:intergenic 20.0%
!!! ACTTTTTTACTTTAACACTT+TGG + chr7.1:28202249-28202268 None:intergenic 20.0%
!!! CATTGAAAATATTTAGCTTT+TGG + chr7.1:28202550-28202569 None:intergenic 20.0%
!!! CTTCATCTTTTAAATGTTTT+CGG - chr7.1:28200985-28201004 MS.gene016246:intron 20.0%
!!! TTTTAGTCAAGTTATTAACA+TGG + chr7.1:28203355-28203374 None:intergenic 20.0%
!!! TTTTTATCTCTATTTTTTCC+GGG + chr7.1:28200795-28200814 None:intergenic 20.0%
! AGAAATATGGCTAAAAAGAA+GGG + chr7.1:28201616-28201635 None:intergenic 25.0%
! AGAATTCTTCAAGAATACTT+AGG - chr7.1:28201674-28201693 MS.gene016246:intron 25.0%
! ATACAATGGATGAAAGTTAT+TGG - chr7.1:28202801-28202820 MS.gene016246:intron 25.0%
! ATATCGTTAGATTCATTACA+TGG + chr7.1:28202437-28202456 None:intergenic 25.0%
! ATCTTTAACTCTTGAATCTA+CGG - chr7.1:28202144-28202163 MS.gene016246:intron 25.0%
! ATTAATATGTAGAACTCACT+TGG + chr7.1:28203319-28203338 None:intergenic 25.0%
! GCTTCATAACTAATAGTATA+GGG + chr7.1:28201819-28201838 None:intergenic 25.0%
! TAGAAATATGGCTAAAAAGA+AGG + chr7.1:28201617-28201636 None:intergenic 25.0%
! TGAGAAATATTTATCAAGGA+GGG + chr7.1:28202121-28202140 None:intergenic 25.0%
! TGCTTCATAACTAATAGTAT+AGG + chr7.1:28201820-28201839 None:intergenic 25.0%
! TGTGTGAGAAATATTTATCA+AGG + chr7.1:28202125-28202144 None:intergenic 25.0%
! TTCTTAGATACAATGAAGTT+TGG + chr7.1:28201590-28201609 None:intergenic 25.0%
!! GTAATTCAAATGTCTTTTAG+TGG + chr7.1:28200825-28200844 None:intergenic 25.0%
!! GTTTAATCAAGTGTGAATTT+TGG - chr7.1:28202378-28202397 MS.gene016246:intron 25.0%
!!! AGTTTACTAGCTTTCTTATT+TGG - chr7.1:28203004-28203023 MS.gene016246:intron 25.0%
!!! CATTGCTCTTTTTAGAAATA+TGG + chr7.1:28201629-28201648 None:intergenic 25.0%
AATTGAATGTTGCAAGGAAA+AGG - chr7.1:28203534-28203553 MS.gene016246:CDS 30.0%
ACCACTAATGTAAACAACAT+AGG - chr7.1:28201889-28201908 MS.gene016246:intron 30.0%
ACCTAAGAATATATGGTCTA+TGG + chr7.1:28202357-28202376 None:intergenic 30.0%
ACTGTTTCATCTAAAAAGAC+TGG + chr7.1:28201430-28201449 None:intergenic 30.0%
AGACAAAGAAGAAGATACAA+TGG - chr7.1:28202787-28202806 MS.gene016246:intron 30.0%
AGATACAATGAAGTTTGGTT+GGG + chr7.1:28201585-28201604 None:intergenic 30.0%
AGCGAAATTATGTAAATAGC+AGG - chr7.1:28202464-28202483 MS.gene016246:intron 30.0%
AGTTTCTTAGAGGTTAATTG+CGG + chr7.1:28203460-28203479 None:intergenic 30.0%
ATATAATAGACCCTATCGTT+TGG - chr7.1:28201748-28201767 MS.gene016246:intron 30.0%
ATTATCCAACCAAACTTCAA+GGG + chr7.1:28201457-28201476 None:intergenic 30.0%
ATTGAATGTTGCAAGGAAAA+GGG - chr7.1:28203535-28203554 MS.gene016246:CDS 30.0%
GAGAAATATTTATCAAGGAG+GGG + chr7.1:28202120-28202139 None:intergenic 30.0%
GAGAAATCTGTTCTTGAATT+TGG - chr7.1:28202893-28202912 MS.gene016246:intron 30.0%
GCGAAATTATGTAAATAGCA+GGG - chr7.1:28202465-28202484 MS.gene016246:intron 30.0%
GTGAGAAATATTTATCAAGG+AGG + chr7.1:28202122-28202141 None:intergenic 30.0%
TAGATACAATGAAGTTTGGT+TGG + chr7.1:28201586-28201605 None:intergenic 30.0%
TCAAGTTATTAACATGGAGA+AGG + chr7.1:28203349-28203368 None:intergenic 30.0%
TCCATAGACCATATATTCTT+AGG - chr7.1:28202353-28202372 MS.gene016246:intron 30.0%
TCTTCAATAGTTGCAATGAA+TGG + chr7.1:28203246-28203265 None:intergenic 30.0%
TCTTTCAGTTCAAAATCAAC+TGG + chr7.1:28202644-28202663 None:intergenic 30.0%
TTGAACATCCTAATCCAAAA+GGG - chr7.1:28203205-28203224 MS.gene016246:intron 30.0%
TTGAATTTGGAGAACAACAT+GGG - chr7.1:28202906-28202925 MS.gene016246:intron 30.0%
TTTACTTTAACACTTTGGAC+CGG + chr7.1:28202244-28202263 None:intergenic 30.0%
! AGTCTTTTTAGATGAAACAG+TGG - chr7.1:28201429-28201448 MS.gene016246:CDS 30.0%
! ATTAACCTCTAAGAAACTCA+TGG - chr7.1:28203462-28203481 MS.gene016246:CDS 30.0%
! TCCTATGTTGTTTACATTAG+TGG + chr7.1:28201893-28201912 None:intergenic 30.0%
! TTATATCTCCCTTTTGGATT+AGG + chr7.1:28203216-28203235 None:intergenic 30.0%
!! AGTTTTAATGCAGCAATTGA+AGG - chr7.1:28202590-28202609 MS.gene016246:intron 30.0%
!! GTTTTAATGCAGCAATTGAA+GGG - chr7.1:28202591-28202610 MS.gene016246:intron 30.0%
AAATGAGTTATGCATGCATG+TGG - chr7.1:28201549-28201568 MS.gene016246:intron 35.0%
AACAAATGGAAGTTGCATTC+AGG - chr7.1:28203096-28203115 MS.gene016246:intron 35.0%
AAGAACAGATTTCTCAGCTA+GGG + chr7.1:28202888-28202907 None:intergenic 35.0%
AATATATGGTCTATGGAGCA+CGG + chr7.1:28202350-28202369 None:intergenic 35.0%
AATATGTTCGACCAAACGAT+AGG + chr7.1:28201762-28201781 None:intergenic 35.0%
AATGAGTTATGCATGCATGT+GGG - chr7.1:28201550-28201569 MS.gene016246:intron 35.0%
AATTCCTAATGCTCCATCAA+TGG + chr7.1:28202693-28202712 None:intergenic 35.0%
ACACCTATATAACACTTCGT+TGG + chr7.1:28202215-28202234 None:intergenic 35.0%
AGTAAACTATGAACAGATCC+AGG + chr7.1:28202992-28203011 None:intergenic 35.0%
ATAATCCAAGAACGTACCAA+TGG + chr7.1:28203044-28203063 None:intergenic 35.0%
ATATGTTCGACCAAACGATA+GGG + chr7.1:28201761-28201780 None:intergenic 35.0%
ATGAAAGGTCCTACAACAAA+AGG + chr7.1:28202956-28202975 None:intergenic 35.0%
ATTAAGAGACTCCTTGAAGA+TGG - chr7.1:28200511-28200530 MS.gene016246:CDS 35.0%
ATTGCTGCATTAAAACTCTC+AGG + chr7.1:28202587-28202606 None:intergenic 35.0%
CATTATCCAACCAAACTTCA+AGG + chr7.1:28201458-28201477 None:intergenic 35.0%
CTTGAACATCCTAATCCAAA+AGG - chr7.1:28203204-28203223 MS.gene016246:intron 35.0%
CTTGAATTTGGAGAACAACA+TGG - chr7.1:28202905-28202924 MS.gene016246:intron 35.0%
GTGACAAAAAGAACCATTGA+TGG - chr7.1:28202677-28202696 MS.gene016246:intron 35.0%
TAATCCAAGAACGTACCAAT+GGG + chr7.1:28203043-28203062 None:intergenic 35.0%
TACTTTCAAAAAGGGTTACC+TGG + chr7.1:28200568-28200587 None:intergenic 35.0%
TTATTGCTTGTATCTCGTAC+CGG - chr7.1:28203138-28203157 MS.gene016246:intron 35.0%
TTGCTGCATTAAAACTCTCA+GGG + chr7.1:28202586-28202605 None:intergenic 35.0%
! ACGAAGTGTTATATAGGTGT+CGG - chr7.1:28202215-28202234 MS.gene016246:intron 35.0%
! ATGTTGGCATTTGAAGTTCA+GGG + chr7.1:28203296-28203315 None:intergenic 35.0%
! CTTTTTGTCACTATTTCCTC+TGG + chr7.1:28202668-28202687 None:intergenic 35.0%
! GAGCAATTATATCTCCCTTT+TGG + chr7.1:28203222-28203241 None:intergenic 35.0%
! TCATCTTCTAGAGAGCTTAT+CGG - chr7.1:28203421-28203440 MS.gene016246:intron 35.0%
! TGTCCAACGAAGTGTTATAT+AGG - chr7.1:28202209-28202228 MS.gene016246:intron 35.0%
! TTTTGAACTGAAAGAACCAG+AGG - chr7.1:28202649-28202668 MS.gene016246:intron 35.0%
AAAGGAAGAGTTTGTGTGAC+TGG - chr7.1:28200460-28200479 MS.gene016246:CDS 40.0%
AAAGGTCCTACAACAAAAGG+TGG + chr7.1:28202953-28202972 None:intergenic 40.0%
AGCATCCATGAGTTTCTTAG+AGG + chr7.1:28203470-28203489 None:intergenic 40.0%
ATCATCAACATGCACCATAC+CGG + chr7.1:28203160-28203179 None:intergenic 40.0%
ATGAGTTATGCATGCATGTG+GGG - chr7.1:28201551-28201570 MS.gene016246:intron 40.0%
ATTTGGAGAACAACATGGGT+TGG - chr7.1:28202910-28202929 MS.gene016246:intron 40.0%
CAAGAACAGATTTCTCAGCT+AGG + chr7.1:28202889-28202908 None:intergenic 40.0%
CATATCGTACGAGTGTCATA+TGG - chr7.1:28202288-28202307 MS.gene016246:intron 40.0%
GGTAAGTGAAAGTTTCCCAT+TGG - chr7.1:28203025-28203044 MS.gene016246:intron 40.0%
GGTACGAGATACAAGCAATA+AGG + chr7.1:28203139-28203158 None:intergenic 40.0%
GGTAGCTTAGGACAAATGAA+AGG + chr7.1:28202971-28202990 None:intergenic 40.0%
TAACAGCGTAACCATCTTCA+AGG + chr7.1:28200525-28200544 None:intergenic 40.0%
TCTAAGAAACTCATGGATGC+TGG - chr7.1:28203469-28203488 MS.gene016246:CDS 40.0%
TGTAGAACTCACTTGGATGT+TGG + chr7.1:28203312-28203331 None:intergenic 40.0%
TTCATTTGTCCTAAGCTACC+TGG - chr7.1:28202971-28202990 MS.gene016246:intron 40.0%
! AGAACCATTGATGGAGCATT+AGG - chr7.1:28202686-28202705 MS.gene016246:intron 40.0%
! CATTTGAAGTTCAGGGTACT+TGG + chr7.1:28203289-28203308 None:intergenic 40.0%
! GATGTTGGCATTTGAAGTTC+AGG + chr7.1:28203297-28203316 None:intergenic 40.0%
! TAGCCTTGACGAAATGTTTG+AGG - chr7.1:28203507-28203526 MS.gene016246:CDS 40.0%
!! CTTGTACCACCTTTTGTTGT+AGG - chr7.1:28202944-28202963 MS.gene016246:intron 40.0%
!! GTACAGGGTTTATTGGTTCA+TGG - chr7.1:28200485-28200504 MS.gene016246:CDS 40.0%
!! TATGGTGCATGTTGATGATG+TGG - chr7.1:28203161-28203180 MS.gene016246:intron 40.0%
!!! GAACTTTGAAGCCTTTTGCT+TGG - chr7.1:28202843-28202862 MS.gene016246:intron 40.0%
!!! TTTAGCTTTTGGGCTGCATT+TGG + chr7.1:28202539-28202558 None:intergenic 40.0%
AATCAACTGGAGTAGCTGTG+TGG + chr7.1:28202631-28202650 None:intergenic 45.0%
ACACCATACGACCAAGCAAA+AGG + chr7.1:28202857-28202876 None:intergenic 45.0%
ACAGTGGTACCCTTGAAGTT+TGG - chr7.1:28201445-28201464 MS.gene016246:CDS 45.0%
ACTCTTGAATCTACGGTGTG+TGG - chr7.1:28202151-28202170 MS.gene016246:intron 45.0%
CGTCCTCAAACATTTCGTCA+AGG + chr7.1:28203513-28203532 None:intergenic 45.0%
CTGCATTTGGAAGATTTGCG+AGG + chr7.1:28202511-28202530 None:intergenic 45.0%
GAGGTTAATTGCGGAAGCTT+AGG + chr7.1:28203451-28203470 None:intergenic 45.0%
GGACGCAATTGAATGTTGCA+AGG - chr7.1:28203528-28203547 MS.gene016246:CDS 45.0%
GTTTCCCATTGGTACGTTCT+TGG - chr7.1:28203036-28203055 MS.gene016246:intron 45.0%
TACGAGTGTCATATGGTGTC+AGG - chr7.1:28202295-28202314 MS.gene016246:intron 45.0%
TGAACAGATCCAGGTAGCTT+AGG + chr7.1:28202983-28203002 None:intergenic 45.0%
TGGTACCCTTGAAGTTTGGT+TGG - chr7.1:28201449-28201468 MS.gene016246:CDS 45.0%
! AAGCCTTTTGCTTGGTCGTA+TGG - chr7.1:28202851-28202870 MS.gene016246:intron 45.0%
! ACTGGAGGTACAGGGTTTAT+TGG - chr7.1:28200478-28200497 MS.gene016246:CDS 45.0%
! CAGGGTACTTGGAAGAAACA+AGG + chr7.1:28203278-28203297 None:intergenic 45.0%
! CTTTTGGGCTGCATTTGGAA+GGG + chr7.1:28202534-28202553 None:intergenic 45.0%
! TCTTCTAGAGAGCTTATCGG+AGG - chr7.1:28203424-28203443 MS.gene016246:intron 45.0%
! TTTGTGTGACTGGAGGTACA+GGG - chr7.1:28200470-28200489 MS.gene016246:CDS 45.0%
!! ATACACAGCAGAAGCACTTG+AGG + chr7.1:28202762-28202781 None:intergenic 45.0%
!! GCATGTTGATGATGTGGCTA+GGG - chr7.1:28203167-28203186 MS.gene016246:intron 45.0%
!! GTTATATAGGTGTCGGACAC+CGG - chr7.1:28202222-28202241 MS.gene016246:intron 45.0%
!! TGCATGTTGATGATGTGGCT+AGG - chr7.1:28203166-28203185 MS.gene016246:intron 45.0%
!! ATATTTCTTAAAAAATATAT+TGG + chr7.1:28201790-28201809 None:intergenic 5.0%
!! TATTTCTTAAAAAATATATT+GGG + chr7.1:28201789-28201808 None:intergenic 5.0%
ACCACTGTTAGAGCTGATCC+AGG - chr7.1:28200547-28200566 MS.gene016246:CDS 50.0%
GCATTTGGAAGGGCTGCATT+TGG + chr7.1:28202524-28202543 None:intergenic 50.0%
GGAAGAGTTTGTGTGACTGG+AGG - chr7.1:28200463-28200482 MS.gene016246:CDS 50.0%
GTCATGTCAGGTGTCTACGT+CGG + chr7.1:28201849-28201868 None:intergenic 50.0%
TATGGAGCACGGACATCTCT+AGG + chr7.1:28202339-28202358 None:intergenic 50.0%
! ACCTGGATCAGCTCTAACAG+TGG + chr7.1:28200551-28200570 None:intergenic 50.0%
! GCAGCAATTGAAGGGTGCAT+TGG - chr7.1:28202599-28202618 MS.gene016246:intron 50.0%
! GCTTGTATCTCGTACCGGTA+TGG - chr7.1:28203143-28203162 MS.gene016246:intron 50.0%
! GCTTTTGGGCTGCATTTGGA+AGG + chr7.1:28202535-28202554 None:intergenic 50.0%
! GTTTGTGTGACTGGAGGTAC+AGG - chr7.1:28200469-28200488 MS.gene016246:CDS 50.0%
! TGGTGTCAACGTGTCTGTGT+CGG + chr7.1:28201873-28201892 None:intergenic 50.0%
!! GTCTGTGTCGGTGTCATGTC+AGG + chr7.1:28201861-28201880 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 28200448 28203567 28200448 ID=MS.gene016246
chr7.1 mRNA 28200448 28203567 28200448 ID=MS.gene016246.t1;Parent=MS.gene016246
chr7.1 exon 28203447 28203567 28203447 ID=MS.gene016246.t1.exon1;Parent=MS.gene016246.t1
chr7.1 CDS 28203447 28203567 28203447 ID=cds.MS.gene016246.t1;Parent=MS.gene016246.t1
chr7.1 exon 28200990 28201532 28200990 ID=MS.gene016246.t1.exon2;Parent=MS.gene016246.t1
chr7.1 CDS 28200990 28201532 28200990 ID=cds.MS.gene016246.t1;Parent=MS.gene016246.t1
chr7.1 exon 28200696 28200897 28200696 ID=MS.gene016246.t1.exon3;Parent=MS.gene016246.t1
chr7.1 CDS 28200696 28200897 28200696 ID=cds.MS.gene016246.t1;Parent=MS.gene016246.t1
chr7.1 exon 28200448 28200583 28200448 ID=MS.gene016246.t1.exon4;Parent=MS.gene016246.t1
chr7.1 CDS 28200448 28200583 28200448 ID=cds.MS.gene016246.t1;Parent=MS.gene016246.t1
Gene Sequence

>MS.gene016246

ATGGAAGAAGAGAAAGGAAGAGTTTGTGTGACTGGAGGTACAGGGTTTATTGGTTCATGGATTATTAAGAGACTCCTTGAAGATGGTTACGCTGTTAATACCACTGTTAGAGCTGATCCAGGTAACCCTTTTTGAAAGTAAAAAAAACTATAAACTAATAAACTACATATTGTTATTGGATTTTAATGATATTATTTAATTTTCATATTGTTTAGTATCTCATTTGAATTAGAATTAAGTTGTACATCGTATTATTAAATTAATTCTTCTTTAATTGTTATTAAAAAAATATCTTCTTCAATTAAAAAAAGTTAAATTACCTTCTTAAAAAAAAAGTTAAATTACCCGGAAAAAATAGAGATAAAAAAAATATGCCACTAAAAGACATTTGAATTACTTTTTTGAATTAACCAAGATAAAAAAAATAGAATAAAAATATATAATACAATAAGACTTAAATAAGTTTTTGGTCTTTATAGATATATCATTTAAAAAAAATATATATAATATTTTCTTTTTTTAGCCGTCTAAATTTTTCTTCATCTTTTAAATGTTTTCGGTCAATATTTTTCATCTTTCTTTTAAGTCAACTCATAAGTATCCTTCAAAATAGATAAATTAAAGTATCCTTCAAATTTCTAAATTATTTTTTGCAGTGAAGTTTAGTAAATTATAAAAATTTCTCTTGGCAAAGTTTAGAATTTTTTAACAAAAGATAAATTAAATATAAATTTTTAAACATGAAAAGTACACACGAGTTCACTTAAAAATAGATATAAAAAATGTTATTGGAAAACTTTTTTTTTAAAGGAATTATTAGAAAAATTTGAATGGCTATTGTTTGAGAAAAAAATGAGGGATTAAAAATGAAACACATTTATAGGAATTAAAAAGTTATTTAACTCTAATAATAAAAAGTATTTTTTATATATATTTAAATAAACTAATATGACAGGTTTAAATAAAAATTGTCTATAATCCAGTCTTTTTAGATGAAACAGTGGTACCCTTGAAGTTTGGTTGGATAATGTACCCATGATCTAATTATACAATGTTATGTCATTTTTTGTATTAATTTATTTAAAGATAAAATCAAATTTTAAATGAGTTATGCATGCATGTGGGGTACGAGTACCCAACCAAACTTCATTGTATCTAAGAAGTTCCCTTCTTTTTAGCCATATTTCTAAAAAGAGCAATGCTATATCTCTAGAAAAATAACATCAAGAATTCTTCAAGAATACTTAGGTTTTGTATTTGAATGAAAGACTAATCTAACTCTAGTCTGACTTAGACAAAGATATAATAGACCCTATCGTTTGGTCGAACATATTCTCACCCAATATATTTTTTAAGAAATATTCTCATCCCTATACTATTAGTTATGAAGCACAAATTCCGACGTAGACACCTGACATGACACCGACACAGACACGTTGACACCACTAATGTAAACAACATAGGACACTGACACCACTACCTATATATTATTAATTTATTTATTAAAGATATAAACTAAGAATCAAAATACATATATATGCATCTAACTTGAGCAATTTATTTCATTAAATAAAGTTCTATAACAAGATACAATAGTATTTGATCTTTATTTATCCATCTACTATAGAAAAATTTAAAAATGTGTTGCAATCAACTTTGATGTCTATAACCCCTCCTTGATAAATATTTCTCACACATCTTTAACTCTTGAATCTACGGTGTGTGGAAGCAGAGAAAAAAAATTCAGATATTTGTCTAAATTGTCCAACGAAGTGTTATATAGGTGTCGGACACCGGTCCAAAGTGTTAAAGTAAAAAAGTAAACAACTTATTTATGACACATATCGTACGAGTGTCATATGGTGTCAGGCATCGCGACACGTGTAATCCTAGAGATGTCCGTGCTCCATAGACCATATATTCTTAGGTTGTTTAATCAAGTGTGAATTTTGGTTATTTGTTGTTATTATAATAGTATTATGTTTTCCATGTAATGAATCTAACGATATCTATGTAAGCGAAATTATGTAAATAGCAGGGCAAAACAAAGATGTTAGCTTCCTCGCAAATCTTCCAAATGCAGCCCTTCCAAATGCAGCCCAAAAGCTAAATATTTTCAATGCTGATCTTAGCTACCCTGAGAGTTTTAATGCAGCAATTGAAGGGTGCATTGGAGTATTCCACACAGCTACTCCAGTTGATTTTGAACTGAAAGAACCAGAGGAAATAGTGACAAAAAGAACCATTGATGGAGCATTAGGAATTCTAAAAGCATGCAAAAATTCAAAGACAGTGAAAAGAGTTGTTTACACCTCAAGTGCTTCTGCTGTGTATACGCAAGACAAAGAAGAAGATACAATGGATGAAAGTTATTGGAGTGATGTGAATATTCTTAGAACTTTGAAGCCTTTTGCTTGGTCGTATGGTGTTTCTAAAACCCTAGCTGAGAAATCTGTTCTTGAATTTGGAGAACAACATGGGTTGGATATTGTGACTCTTGTACCACCTTTTGTTGTAGGACCTTTCATTTGTCCTAAGCTACCTGGATCTGTTCATAGTTTACTAGCTTTCTTATTTGGTAAGTGAAAGTTTCCCATTGGTACGTTCTTGGATTATTTTGAAAGTATTTTCTTAAATAGATGAAAATTAACAAATGGAAGTTGCATTCAGGTGATATTGAGAAGCATTCCTTATTGCTTGTATCTCGTACCGGTATGGTGCATGTTGATGATGTGGCTAGGGCACATATATTCTTACTTGAACATCCTAATCCAAAAGGGAGATATAATTGCTCACCATTCATTGCAACTATTGAAGAAATAGTTGACCTTGTTTCTTCCAAGTACCCTGAACTTCAAATGCCAACATCCAAGTGAGTTCTACATATTAATTACATGTCCTTCTCCATGTTAATAACTTGACTAAAATTATGTGATCTTTCTTTTATTGTTACTAATACTATTCAATTTGTGTTCATCTTCTAGAGAGCTTATCGGAGGTCCTAAGCTTCCGCAATTAACCTCTAAGAAACTCATGGATGCTGGATTCAAGTTTAAATATAGCCTTGACGAAATGTTTGAGGACGCAATTGAATGTTGCAAGGAAAAGGGTTATCTTTGA
Protein sequence

>MS.gene016246.t1

MEEEKGRVCVTGGTGFIGSWIIKRLLEDGYAVNTTVRADPAGQNKDVSFLANLPNAALPNAAQKLNIFNADLSYPESFNAAIEGCIGVFHTATPVDFELKEPEEIVTKRTIDGALGILKACKNSKTVKRVVYTSSASAVYTQDKEEDTMDESYWSDVNILRTLKPFAWSYGVSKTLAEKSVLEFGEQHGLDIVTLVPPFVVGPFICPKLPGSVHSLLAFLFGDIEKHSLLLVSRTGMVHVDDVARAHIFLLEHPNPKGRYNCSPFIATIEEIVDLVSSKYPELQMPTSKELIGGPKLPQLTSKKLMDAGFKFKYSLDEMFEDAIECCKEKGYL