AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071401

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071401.t1 MTR_4g092080 94.940 336 15 1 2 335 1 336 0.0 654
MS.gene071401.t1 MTR_1g022440 42.249 329 179 4 13 339 8 327 3.98e-96 288
MS.gene071401.t1 MTR_1g022445 43.598 328 174 4 14 339 9 327 6.66e-95 285
MS.gene071401.t1 MTR_7g074820 40.964 332 185 6 10 337 5 329 2.78e-80 248
MS.gene071401.t1 MTR_7g074730 37.952 332 194 5 10 337 5 328 4.61e-78 242
MS.gene071401.t1 MTR_7g074880 39.157 332 188 6 10 337 5 326 1.59e-77 241
MS.gene071401.t1 MTR_7g074850 39.759 332 189 6 10 337 5 329 5.41e-77 239
MS.gene071401.t1 MTR_7g074710 37.349 332 194 5 10 337 5 326 6.00e-74 231
MS.gene071401.t1 MTR_4g081440 38.344 326 189 4 12 328 23 345 1.39e-72 229
MS.gene071401.t1 MTR_4g081440 38.690 336 173 7 12 328 19 340 2.48e-72 228
MS.gene071401.t1 MTR_2g101330 36.970 330 194 8 10 337 2 319 1.62e-65 210
MS.gene071401.t1 MTR_8g062440 39.216 306 171 5 9 312 4 296 5.62e-65 208
MS.gene071401.t1 MTR_3g005210 38.509 322 182 7 14 332 7 315 6.22e-65 208
MS.gene071401.t1 MTR_8g062440 39.871 311 171 6 9 316 4 301 9.53e-65 207
MS.gene071401.t1 MTR_4g077100 39.498 319 172 7 4 316 2 305 9.18e-64 205
MS.gene071401.t1 MTR_4g077130 39.490 314 169 7 9 316 5 303 1.69e-63 204
MS.gene071401.t1 MTR_4g077100 40.830 289 157 5 4 290 2 278 2.58e-63 202
MS.gene071401.t1 MTR_3g005170 37.654 324 186 7 14 334 11 321 4.52e-63 203
MS.gene071401.t1 MTR_3g005170 40.502 279 152 5 14 290 11 277 5.50e-63 202
MS.gene071401.t1 MTR_8g062440 39.744 312 171 7 9 316 4 302 8.32e-63 202
MS.gene071401.t1 MTR_8g062110 37.690 329 188 7 9 334 5 319 1.26e-62 202
MS.gene071401.t1 MTR_8g062110 37.920 327 186 7 9 332 14 326 1.36e-62 202
MS.gene071401.t1 MTR_8g062110 39.929 283 155 5 9 289 5 274 6.11e-62 199
MS.gene071401.t1 MTR_8g062110 39.929 283 155 5 9 289 5 274 7.59e-62 199
MS.gene071401.t1 MTR_4g009040 38.372 344 186 11 1 338 1 324 7.56e-58 190
MS.gene071401.t1 MTR_7g074870 36.299 281 163 4 61 337 20 288 3.22e-57 187
MS.gene071401.t1 MTR_4g023730 34.030 335 206 7 9 337 4 329 5.50e-56 185
MS.gene071401.t1 MTR_4g009040 37.931 348 186 12 1 338 1 328 1.18e-55 184
MS.gene071401.t1 MTR_5g056600 33.333 330 199 9 14 337 5 319 3.83e-54 180
MS.gene071401.t1 MTR_4g009690 37.901 343 188 10 1 338 1 323 2.45e-51 173
MS.gene071401.t1 MTR_2g013230 31.045 335 221 6 12 337 7 340 4.96e-51 173
MS.gene071401.t1 MTR_5g072620 33.731 335 196 9 11 337 3 319 6.06e-51 172
MS.gene071401.t1 MTR_3g005170 34.259 324 166 8 14 334 11 290 2.61e-50 169
MS.gene071401.t1 MTR_4g009690 37.791 344 188 11 1 338 1 324 1.24e-49 169
MS.gene071401.t1 MTR_2g104960 37.920 327 182 11 14 337 21 329 1.26e-49 169
MS.gene071401.t1 MTR_2g013260 31.343 335 216 7 12 335 7 338 1.15e-48 167
MS.gene071401.t1 MTR_4g006940 35.474 327 190 10 14 337 18 326 7.91e-48 164
MS.gene071401.t1 MTR_3g031650 31.940 335 198 9 11 335 3 317 2.89e-47 162
MS.gene071401.t1 MTR_4g006940 37.102 283 160 7 11 292 15 280 3.49e-47 161
MS.gene071401.t1 MTR_4g006940 37.102 283 160 7 11 292 15 280 3.49e-47 161
MS.gene071401.t1 MTR_2g013250 30.060 336 225 6 9 335 3 337 2.23e-46 160
MS.gene071401.t1 MTR_7g117190 30.973 339 217 7 12 337 6 340 3.06e-46 160
MS.gene071401.t1 MTR_2g013260 32.872 289 181 6 12 289 7 293 1.30e-44 155
MS.gene071401.t1 MTR_3g031650 32.168 286 170 6 11 289 3 271 3.42e-44 153
MS.gene071401.t1 MTR_7g117190 31.650 297 187 6 10 293 4 297 7.90e-42 148
MS.gene071401.t1 MTR_2g028620 31.845 336 201 14 14 339 11 328 1.22e-40 145
MS.gene071401.t1 MTR_2g028620 33.569 283 166 11 14 289 11 278 1.03e-39 142
MS.gene071401.t1 MTR_8g062110 34.764 233 136 6 102 332 13 231 4.35e-32 120
MS.gene071401.t1 MTR_8g062110 36.649 191 107 4 100 289 11 188 2.45e-31 117
MS.gene071401.t1 MTR_2g013230 28.033 239 163 5 107 337 13 250 1.20e-27 108
MS.gene071401.t1 MTR_0164s0070 38.411 151 89 4 10 156 2 152 2.26e-26 104
MS.gene071401.t1 MTR_2g013260 28.452 239 158 6 107 335 32 267 1.08e-24 101
MS.gene071401.t1 MTR_7g117190 28.017 232 151 6 118 337 3 230 1.03e-21 92.4
MS.gene071401.t1 MTR_6g406250 27.372 274 165 8 6 266 48 300 5.17e-21 92.8
MS.gene071401.t1 MTR_3g011130 41.176 119 65 4 103 219 30 145 6.19e-17 77.0
MS.gene071401.t1 MTR_6g406250 26.977 215 140 5 6 211 48 254 1.08e-16 79.0
MS.gene071401.t1 MTR_0685s0030 33.553 152 85 3 169 316 3 142 4.19e-16 75.1
MS.gene071401.t1 MTR_0685s0030 35.246 122 70 1 169 290 3 115 1.82e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071401.t1 AT1G61720 59.467 338 137 0 1 338 1 338 1.11e-144 412
MS.gene071401.t1 AT5G42800 42.296 331 180 4 9 337 4 325 2.05e-95 288
MS.gene071401.t1 AT4G35420 40.729 329 182 7 10 337 5 321 1.72e-75 235
MS.gene071401.t1 AT4G27250 38.110 328 188 7 14 329 14 338 2.59e-66 213
MS.gene071401.t1 AT1G51410 37.092 337 188 9 9 338 5 324 3.00e-64 206
MS.gene071401.t1 AT2G45400 35.952 331 198 6 14 337 41 364 1.50e-62 203
MS.gene071401.t1 AT4G27250 38.110 328 178 10 14 329 14 328 3.19e-62 202
MS.gene071401.t1 AT1G09510 41.667 276 147 5 11 284 6 269 6.62e-62 200
MS.gene071401.t1 AT5G19440 36.667 330 193 7 9 335 6 322 1.90e-59 194
MS.gene071401.t1 AT1G09490 40.071 282 153 6 11 289 6 274 1.11e-58 192
MS.gene071401.t1 AT1G66800 36.310 336 186 9 9 337 4 318 2.66e-56 186
MS.gene071401.t1 AT1G09480 40.000 275 149 6 11 282 6 267 1.09e-55 184
MS.gene071401.t1 AT2G45400 37.324 284 165 5 14 291 41 317 1.15e-55 184
MS.gene071401.t1 AT1G09480 40.000 275 149 6 11 282 53 314 2.22e-55 185
MS.gene071401.t1 AT2G33590 36.728 324 188 9 8 329 4 312 2.46e-55 183
MS.gene071401.t1 AT2G33600 37.654 324 185 9 8 329 4 312 5.26e-55 182
MS.gene071401.t1 AT1G09500 39.007 282 147 7 11 284 6 270 5.36e-55 181
MS.gene071401.t1 AT1G09500 41.445 263 129 7 11 265 6 251 1.96e-54 181
MS.gene071401.t1 AT1G68540 32.326 331 201 11 15 337 6 321 6.88e-49 167
MS.gene071401.t1 AT2G02400 31.765 340 200 12 8 337 1 318 1.97e-48 165
MS.gene071401.t1 AT2G45400 33.562 292 182 5 51 337 23 307 2.27e-48 165
MS.gene071401.t1 AT5G58490 33.436 326 179 10 9 313 5 313 1.55e-47 163
MS.gene071401.t1 AT1G15950 37.500 296 167 8 1 295 1 279 1.01e-46 160
MS.gene071401.t1 AT1G76470 35.329 334 199 8 10 339 4 324 1.15e-46 161
MS.gene071401.t1 AT1G25460 32.012 328 204 8 15 337 6 319 2.66e-46 160
MS.gene071401.t1 AT1G15950 36.061 330 190 11 11 337 11 322 1.83e-45 158
MS.gene071401.t1 AT1G15950 36.061 330 190 11 11 337 11 322 1.83e-45 158
MS.gene071401.t1 AT1G80820 35.435 333 194 11 8 337 3 317 4.18e-45 157
MS.gene071401.t1 AT1G15950 36.164 318 183 10 11 325 11 311 1.59e-44 155
MS.gene071401.t1 AT1G15950 36.860 293 167 8 1 292 1 276 3.67e-44 153
MS.gene071401.t1 AT1G15950 36.986 292 166 8 1 291 1 275 5.45e-44 153
MS.gene071401.t1 AT1G80820 37.413 286 161 8 8 292 3 271 1.60e-43 151
MS.gene071401.t1 AT1G09490 38.525 244 134 6 49 289 13 243 1.70e-43 152
MS.gene071401.t1 AT1G09500 40.000 230 113 7 44 265 5 217 2.45e-40 143
MS.gene071401.t1 AT2G45400 39.070 215 125 4 14 222 41 255 1.59e-39 140
MS.gene071401.t1 AT2G23910 27.344 256 166 8 13 266 10 247 1.96e-25 103
MS.gene071401.t1 AT2G23910 27.344 256 166 8 13 266 10 247 2.11e-25 103
MS.gene071401.t1 AT2G23910 27.344 256 166 8 13 266 10 247 2.29e-25 103
MS.gene071401.t1 AT4G30470 25.391 256 170 7 13 266 10 246 6.25e-24 100
MS.gene071401.t1 AT1G15950 32.489 237 145 7 103 337 1 224 3.21e-23 97.1
MS.gene071401.t1 AT1G68540 27.193 228 147 8 115 337 1 214 1.94e-22 94.0
MS.gene071401.t1 AT2G23910 32.075 159 94 4 108 266 69 213 7.67e-20 88.2
MS.gene071401.t1 AT5G14700 28.195 266 162 10 14 266 56 305 2.90e-19 88.2
MS.gene071401.t1 AT5G14700 30.151 199 128 7 14 204 56 251 1.15e-16 79.7
MS.gene071401.t1 AT4G33360 27.985 268 164 9 7 272 9 249 2.03e-15 76.3
MS.gene071401.t1 AT4G33360 27.985 268 164 9 7 272 9 249 3.09e-15 76.3
MS.gene071401.t1 AT4G33360 28.137 263 160 9 12 272 2 237 1.27e-14 73.9

Find 56 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATCAACTTTGCCTTGC+TGG 0.226247 4.2:-20461164 None:intergenic
CCTCATAGCACTGCAAAGTT+TGG 0.275725 4.2:+20459503 MS.gene071401:CDS
CGAAGGGACTGGTCATATTA+TGG 0.276006 4.2:+20459900 MS.gene071401:CDS
TTCAATACACCTTTGATTGC+TGG 0.333253 4.2:-20459793 None:intergenic
TCTTCAAATACTCCAGAGTT+TGG 0.365279 4.2:-20461249 None:intergenic
TTCCTTTCAAAGCATTTATG+AGG 0.388148 4.2:-20460732 None:intergenic
ATTCCCATGGAAACACTAGA+TGG 0.394524 4.2:-20460593 None:intergenic
TCTGAAAAGCTTATCAAAGA+AGG 0.411453 4.2:+20461190 MS.gene071401:CDS
AAGAAGAAGGCATGTGTGAT+AGG 0.414338 4.2:+20458867 MS.gene071401:CDS
CTCATAGCACTGCAAAGTTT+GGG 0.415377 4.2:+20459504 MS.gene071401:CDS
TATCAAAATCTACTTCTTCA+AGG 0.417228 4.2:-20461281 None:intergenic
ATGGGAATGTCACTTCTAAC+AGG 0.424075 4.2:+20460608 MS.gene071401:CDS
CTCAAAAGTGCTAGCTGAAA+AGG 0.425883 4.2:+20460481 MS.gene071401:CDS
GTGATACCTACTCTAACAAT+TGG 0.428631 4.2:+20460548 MS.gene071401:CDS
ACAAGTTCACATCCTGCTAT+AGG 0.437816 4.2:-20459579 None:intergenic
GATCAAGCAGTTGCTTGAAA+AGG 0.456606 4.2:+20458917 MS.gene071401:CDS
AAAGGAATGCAGATCCTGTC+AGG 0.463412 4.2:+20460748 MS.gene071401:CDS
CTGAGAAACTTAGCAAGCTC+AGG 0.468414 4.2:-20460880 None:intergenic
ATCAAGCAGTTGCTTGAAAA+GGG 0.470848 4.2:+20458918 MS.gene071401:CDS
TATCCCATCTAGTGTTTCCA+TGG 0.473150 4.2:+20460589 MS.gene071401:CDS
TTCCTCATAAATGCTTTGAA+AGG 0.475020 4.2:+20460730 MS.gene071401:CDS
TGACTTCAAGCTTACTCAGT+TGG 0.478141 4.2:-20460928 None:intergenic
CCAAACTTTGCAGTGCTATG+AGG 0.494447 4.2:-20459503 None:intergenic
ATATTATGGATGAAACCAAC+TGG 0.503991 4.2:+20459914 MS.gene071401:CDS
CTGAAAAGCTTATCAAAGAA+GGG 0.506098 4.2:+20461191 MS.gene071401:CDS
TCTTGAGAGGCAAAGTTCAC+AGG 0.513934 4.2:-20459618 None:intergenic
CACTGCAAAGCCACCCACTT+GGG 0.520500 4.2:+20459957 MS.gene071401:CDS
TCTGGAGTATTTGAAGACTC+AGG 0.526497 4.2:+20461255 MS.gene071401:CDS
CTGCTACAAATATATGAGCT+CGG 0.536593 4.2:-20460804 None:intergenic
TGCTAGCTGAAAAGGCTGCA+TGG 0.537823 4.2:+20460489 MS.gene071401:CDS
TGATGGATATCGAACCTGAC+AGG 0.542607 4.2:-20460762 None:intergenic
CTGGAGTATTTGAAGACTCA+GGG 0.551188 4.2:+20461256 MS.gene071401:CDS
AGTTCACAGGTGTAGCAAGT+TGG 0.553446 4.2:-20459605 None:intergenic
GAAGGACCAATTGTTAGAGT+AGG 0.556874 4.2:-20460554 None:intergenic
CAAACAAATAGAAAAGAAGA+AGG 0.557146 4.2:+20458854 MS.gene071401:CDS
AAACTCAACATCAGACCAGT+TGG 0.559402 4.2:-20459929 None:intergenic
GCATGTGTGATAGGTGGCAC+TGG 0.564473 4.2:+20458876 MS.gene071401:CDS
ATGATAAAACCAGCAATCAA+AGG 0.568131 4.2:+20459784 MS.gene071401:CDS
ACACTGCAAAGCCACCCACT+TGG 0.573692 4.2:+20459956 MS.gene071401:CDS
AATATCCTCGACATGAGTGA+TGG 0.579257 4.2:-20460779 None:intergenic
ATAAACGAACTCGAAGGGAC+TGG 0.592956 4.2:+20459889 MS.gene071401:CDS
ATTTGATGATTGCTCCAGCA+AGG 0.593062 4.2:+20461150 MS.gene071401:CDS
ATCCCATCTAGTGTTTCCAT+GGG 0.594221 4.2:+20460590 MS.gene071401:CDS
GGGATATCTTGTGTTAGAGA+AGG 0.600247 4.2:-20460572 None:intergenic
GAAGGGTTCAGTTTCAAGCA+TGG 0.601008 4.2:+20461208 MS.gene071401:CDS
AATACTACTGTTAGAGACCC+AGG 0.604643 4.2:+20458951 MS.gene071401:CDS
ACCTACCTCGGGATCTTGAG+AGG 0.609339 4.2:-20459631 None:intergenic
TTCCCATGGAAACACTAGAT+GGG 0.620353 4.2:-20460592 None:intergenic
GTGACTATAAACGAACTCGA+AGG 0.630121 4.2:+20459883 MS.gene071401:CDS
TGACTATAAACGAACTCGAA+GGG 0.632588 4.2:+20459884 MS.gene071401:CDS
CTTTGCCTCTCAAGATCCCG+AGG 0.635384 4.2:+20459626 MS.gene071401:CDS
TGTTAGAAGTGACATTCCCA+TGG 0.640999 4.2:-20460606 None:intergenic
GATATCCATCACTCATGTCG+AGG 0.645745 4.2:+20460774 MS.gene071401:CDS
AAGAAGGCATGTGTGATAGG+TGG 0.652368 4.2:+20458870 MS.gene071401:CDS
GGAGTATTTGAAGACTCAGG+GGG 0.662530 4.2:+20461258 MS.gene071401:CDS
TGGAGTATTTGAAGACTCAG+GGG 0.686930 4.2:+20461257 MS.gene071401:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTCAAAAATTAATTCAATT+AGG - chr4.2:20459696-20459715 None:intergenic 15.0%
!! TGCAATTCATATATATATTA+TGG - chr4.2:20460310-20460329 None:intergenic 15.0%
!!! AGATTATTTTGACATAAAAT+GGG + chr4.2:20460008-20460027 MS.gene071401:intron 15.0%
!!! CACACAATATTTTAATATTT+TGG - chr4.2:20459059-20459078 None:intergenic 15.0%
!! AATCCTTAAACCATTTAATT+TGG - chr4.2:20460644-20460663 None:intergenic 20.0%
!! ATAACCATTTAAATTGTTGT+CGG - chr4.2:20460044-20460063 None:intergenic 20.0%
!! TAAAATTGAATACATGGTTA+TGG + chr4.2:20460070-20460089 MS.gene071401:intron 20.0%
!! TATGGTTAAGATTTCAAATA+AGG + chr4.2:20460088-20460107 MS.gene071401:intron 20.0%
!!! ATCTCAATTTTATATTTTGC+AGG + chr4.2:20460700-20460719 MS.gene071401:intron 20.0%
!!! ATTTTTGAAGTATATGCATT+TGG + chr4.2:20459706-20459725 MS.gene071401:intron 20.0%
!!! CAGATTATTTTGACATAAAA+TGG + chr4.2:20460007-20460026 MS.gene071401:intron 20.0%
!!! CATTTTATGTCAAAATAATC+TGG - chr4.2:20460009-20460028 None:intergenic 20.0%
!!! TAGATCAATGTTATTTTCTT+CGG - chr4.2:20460522-20460541 None:intergenic 20.0%
!!! TCGTGTTTAATTTTTTTCAA+TGG + chr4.2:20460265-20460284 MS.gene071401:intron 20.0%
! AAAAAATGAAACTTTGCCTT+AGG - chr4.2:20461122-20461141 None:intergenic 25.0%
! AAATTATCATCAAAATGTGC+AGG + chr4.2:20460448-20460467 MS.gene071401:intron 25.0%
! AATGATTATTTCATAGTGCT+CGG + chr4.2:20461020-20461039 MS.gene071401:intron 25.0%
! AATTATCATCAAAATGTGCA+GGG + chr4.2:20460449-20460468 MS.gene071401:intron 25.0%
! ATACATTACCTATCTTTCAA+AGG + chr4.2:20461070-20461089 MS.gene071401:intron 25.0%
! ATCATTTATGTACTCAACTT+AGG + chr4.2:20460386-20460405 MS.gene071401:intron 25.0%
! CAAACAAATAGAAAAGAAGA+AGG + chr4.2:20458854-20458873 MS.gene071401:CDS 25.0%
! CATATATATATTATGGTCAG+TGG - chr4.2:20460303-20460322 None:intergenic 25.0%
! CGATAAATTTGACAAAATCA+TGG - chr4.2:20459346-20459365 None:intergenic 25.0%
! TAATTTGGTCAAAATTACAG+TGG - chr4.2:20459315-20459334 None:intergenic 25.0%
! TGAAAAACAGCCAAATTAAA+TGG + chr4.2:20460631-20460650 MS.gene071401:intron 25.0%
! TGAATTAGCGATTAAAAAAC+TGG - chr4.2:20459085-20459104 None:intergenic 25.0%
! TTCAAATTGTTGTCGATATT+TGG + chr4.2:20460343-20460362 MS.gene071401:intron 25.0%
!! AGATTTCAAATAAGGTATCA+TGG + chr4.2:20460096-20460115 MS.gene071401:intron 25.0%
!! CCAATTAGAAATGATAATGA+GGG - chr4.2:20460425-20460444 None:intergenic 25.0%
!! GCAGTTTAAAATTGAATACA+TGG + chr4.2:20460064-20460083 MS.gene071401:intron 25.0%
!! TCCAATTAGAAATGATAATG+AGG - chr4.2:20460426-20460445 None:intergenic 25.0%
!!! CTGTAATTTTGACCAAATTA+CGG + chr4.2:20459315-20459334 MS.gene071401:intron 25.0%
!!! GTTTTTTAATCGCTAATTCA+TGG + chr4.2:20459085-20459104 MS.gene071401:intron 25.0%
AAATGGTTTAAGGATTTCCA+AGG + chr4.2:20460648-20460667 MS.gene071401:intron 30.0%
ACGTATATTTGAACACAGTT+TGG + chr4.2:20460181-20460200 MS.gene071401:intron 30.0%
ATATTATGGATGAAACCAAC+TGG + chr4.2:20459914-20459933 MS.gene071401:CDS 30.0%
ATGATAAAACCAGCAATCAA+AGG + chr4.2:20459784-20459803 MS.gene071401:CDS 30.0%
ATGTTCAGTGAATGGTTAAT+AGG - chr4.2:20460122-20460141 None:intergenic 30.0%
CAGCCAAATTAAATGGTTTA+AGG + chr4.2:20460638-20460657 MS.gene071401:intron 30.0%
CCCTCATTATCATTTCTAAT+TGG + chr4.2:20460422-20460441 MS.gene071401:intron 30.0%
CTGAAAAGCTTATCAAAGAA+GGG + chr4.2:20461191-20461210 MS.gene071401:CDS 30.0%
GAAATAATCATTGTTCTCTG+TGG - chr4.2:20461012-20461031 None:intergenic 30.0%
GAGAATGACATTCAAAATCT+TGG - chr4.2:20460151-20460170 None:intergenic 30.0%
GTTTCCGACAACAATTTAAA+TGG + chr4.2:20460037-20460056 MS.gene071401:intron 30.0%
TCAAATATACGTGAAAAACG+TGG - chr4.2:20460173-20460192 None:intergenic 30.0%
TCTGAAAAGCTTATCAAAGA+AGG + chr4.2:20461190-20461209 MS.gene071401:CDS 30.0%
! AGGAAAATCCTTTGAAAGAT+AGG - chr4.2:20461081-20461100 None:intergenic 30.0%
! GACAACAATTTGAAACTGAA+TGG - chr4.2:20460337-20460356 None:intergenic 30.0%
! GACACATGCAAATTCTTTTT+GGG - chr4.2:20459163-20459182 None:intergenic 30.0%
! TGACACATGCAAATTCTTTT+TGG - chr4.2:20459164-20459183 None:intergenic 30.0%
! TTCCTCATAAATGCTTTGAA+AGG + chr4.2:20460730-20460749 MS.gene071401:CDS 30.0%
! TTCCTTTCAAAGCATTTATG+AGG - chr4.2:20460735-20460754 None:intergenic 30.0%
!! AAGTTTGATTTTTACCCAAG+TGG - chr4.2:20459974-20459993 None:intergenic 30.0%
!! AGTTTGATTTTTACCCAAGT+GGG - chr4.2:20459973-20459992 None:intergenic 30.0%
!!! CGCTATTGAAGTAGTTTTAT+AGG + chr4.2:20459395-20459414 MS.gene071401:intron 30.0%
AACTACTTCAATAGCGAGAT+AGG - chr4.2:20459391-20459410 None:intergenic 35.0%
ATCAAGCAGTTGCTTGAAAA+GGG + chr4.2:20458918-20458937 MS.gene071401:CDS 35.0%
ATCTTAAACCTCATTGACCA+CGG - chr4.2:20460246-20460265 None:intergenic 35.0%
CTGCTACAAATATATGAGCT+CGG - chr4.2:20460807-20460826 None:intergenic 35.0%
GGTTAATGATGTTCAGTGAA+TGG - chr4.2:20460130-20460149 None:intergenic 35.0%
GTGATACCTACTCTAACAAT+TGG + chr4.2:20460548-20460567 MS.gene071401:CDS 35.0%
TCTTCAAATACTCCAGAGTT+TGG - chr4.2:20461252-20461271 None:intergenic 35.0%
TGACTATAAACGAACTCGAA+GGG + chr4.2:20459884-20459903 MS.gene071401:CDS 35.0%
TGTGCTTAAGAGATTGTGTT+TGG - chr4.2:20459271-20459290 None:intergenic 35.0%
TTCAATACACCTTTGATTGC+TGG - chr4.2:20459796-20459815 None:intergenic 35.0%
! ATCAGCATTAGTTTCTCCTA+AGG + chr4.2:20461103-20461122 MS.gene071401:intron 35.0%
! TAGAAATGATAATGAGGGCA+GGG - chr4.2:20460420-20460439 None:intergenic 35.0%
! TGAAACTTTTGACCAAACTC+TGG + chr4.2:20461237-20461256 MS.gene071401:CDS 35.0%
! TTAGAAATGATAATGAGGGC+AGG - chr4.2:20460421-20460440 None:intergenic 35.0%
!! GTCTCAAAACAAGAAGTACT+AGG + chr4.2:20459365-20459384 MS.gene071401:intron 35.0%
!!! TCTTAAGCACATGTTTTGAC+CGG + chr4.2:20459280-20459299 MS.gene071401:intron 35.0%
AAACTCAACATCAGACCAGT+TGG - chr4.2:20459932-20459951 None:intergenic 40.0%
AATACTACTGTTAGAGACCC+AGG + chr4.2:20458951-20458970 MS.gene071401:CDS 40.0%
AATATCCTCGACATGAGTGA+TGG - chr4.2:20460782-20460801 None:intergenic 40.0%
ACAAGTTCACATCCTGCTAT+AGG - chr4.2:20459582-20459601 None:intergenic 40.0%
AGATGTTAAGAGAGAGACCT+GGG - chr4.2:20458971-20458990 None:intergenic 40.0%
ATCATGGTGACACCGTAATT+TGG - chr4.2:20459330-20459349 None:intergenic 40.0%
ATCCCATCTAGTGTTTCCAT+GGG + chr4.2:20460590-20460609 MS.gene071401:CDS 40.0%
ATTCCCATGGAAACACTAGA+TGG - chr4.2:20460596-20460615 None:intergenic 40.0%
CTCATAGCACTGCAAAGTTT+GGG + chr4.2:20459504-20459523 MS.gene071401:CDS 40.0%
GAAGGACCAATTGTTAGAGT+AGG - chr4.2:20460557-20460576 None:intergenic 40.0%
GAGTCTTTGTGTATTGTCCT+TGG - chr4.2:20460668-20460687 None:intergenic 40.0%
GATCAAGCAGTTGCTTGAAA+AGG + chr4.2:20458917-20458936 MS.gene071401:CDS 40.0%
GATTATCAACTTTGCCTTGC+TGG - chr4.2:20461167-20461186 None:intergenic 40.0%
GCAGAAAAAGAATCAGCTTC+TGG + chr4.2:20460823-20460842 MS.gene071401:CDS 40.0%
GGAGAAACTAATGCTGATGA+AGG - chr4.2:20461101-20461120 None:intergenic 40.0%
GGGATATCTTGTGTTAGAGA+AGG - chr4.2:20460575-20460594 None:intergenic 40.0%
GTGACTATAAACGAACTCGA+AGG + chr4.2:20459883-20459902 MS.gene071401:CDS 40.0%
GTTTAGCAATCAACCTACCT+CGG - chr4.2:20459646-20459665 None:intergenic 40.0%
TATCCCATCTAGTGTTTCCA+TGG + chr4.2:20460589-20460608 MS.gene071401:CDS 40.0%
TGACTTCAAGCTTACTCAGT+TGG - chr4.2:20460931-20460950 None:intergenic 40.0%
TGTTAGAAGTGACATTCCCA+TGG - chr4.2:20460609-20460628 None:intergenic 40.0%
TTCCCATGGAAACACTAGAT+GGG - chr4.2:20460595-20460614 None:intergenic 40.0%
TTTAGCAATCAACCTACCTC+GGG - chr4.2:20459645-20459664 None:intergenic 40.0%
TTTGAACTTTGCAGCTTCAG+AGG - chr4.2:20460983-20461002 None:intergenic 40.0%
! AAGAAGAAGGCATGTGTGAT+AGG + chr4.2:20458867-20458886 MS.gene071401:CDS 40.0%
! ATGGGAATGTCACTTCTAAC+AGG + chr4.2:20460608-20460627 MS.gene071401:CDS 40.0%
! ATTTGATGATTGCTCCAGCA+AGG + chr4.2:20461150-20461169 MS.gene071401:CDS 40.0%
! CTGGAGTATTTGAAGACTCA+GGG + chr4.2:20461256-20461275 MS.gene071401:CDS 40.0%
! TCTGGAGTATTTGAAGACTC+AGG + chr4.2:20461255-20461274 MS.gene071401:CDS 40.0%
! TGGAGTATTTGAAGACTCAG+GGG + chr4.2:20461257-20461276 MS.gene071401:CDS 40.0%
! TTGATTTTTACCCAAGTGGG+TGG - chr4.2:20459970-20459989 None:intergenic 40.0%
!! CTCAAAAGTGCTAGCTGAAA+AGG + chr4.2:20460481-20460500 MS.gene071401:CDS 40.0%
!! GATTTTGATGCTCCTATAGC+AGG + chr4.2:20459567-20459586 MS.gene071401:CDS 40.0%
AAAGGAATGCAGATCCTGTC+AGG + chr4.2:20460748-20460767 MS.gene071401:CDS 45.0%
AGTTCACAGGTGTAGCAAGT+TGG - chr4.2:20459608-20459627 None:intergenic 45.0%
ATAAACGAACTCGAAGGGAC+TGG + chr4.2:20459889-20459908 MS.gene071401:CDS 45.0%
ATTACAGTGGCACACTTCTC+CGG - chr4.2:20459302-20459321 None:intergenic 45.0%
ATTTGAACACCTGAAAGCCG+TGG + chr4.2:20460226-20460245 MS.gene071401:intron 45.0%
CCAAACTTTGCAGTGCTATG+AGG - chr4.2:20459506-20459525 None:intergenic 45.0%
CCTCATAGCACTGCAAAGTT+TGG + chr4.2:20459503-20459522 MS.gene071401:CDS 45.0%
CTGAGAAACTTAGCAAGCTC+AGG - chr4.2:20460883-20460902 None:intergenic 45.0%
GAAGGGTTCAGTTTCAAGCA+TGG + chr4.2:20461208-20461227 MS.gene071401:CDS 45.0%
GAGATGTTAAGAGAGAGACC+TGG - chr4.2:20458972-20458991 None:intergenic 45.0%
GATATCCATCACTCATGTCG+AGG + chr4.2:20460774-20460793 MS.gene071401:CDS 45.0%
TCTTGAGAGGCAAAGTTCAC+AGG - chr4.2:20459621-20459640 None:intergenic 45.0%
! AAGAAGGCATGTGTGATAGG+TGG + chr4.2:20458870-20458889 MS.gene071401:CDS 45.0%
! GGAGTATTTGAAGACTCAGG+GGG + chr4.2:20461258-20461277 MS.gene071401:CDS 45.0%
! TGATGGATATCGAACCTGAC+AGG - chr4.2:20460765-20460784 None:intergenic 45.0%
!! CGAAGGGACTGGTCATATTA+TGG + chr4.2:20459900-20459919 MS.gene071401:CDS 45.0%
!!! TCAGCTAGCACTTTTGAGAC+AGG - chr4.2:20460479-20460498 None:intergenic 45.0%
TGCTAGCTGAAAAGGCTGCA+TGG + chr4.2:20460489-20460508 MS.gene071401:CDS 50.0%
!!! GATAGGTGGCACTGGTTTTG+TGG + chr4.2:20458884-20458903 MS.gene071401:CDS 50.0%
ACACTGCAAAGCCACCCACT+TGG + chr4.2:20459956-20459975 MS.gene071401:CDS 55.0%
ACCTACCTCGGGATCTTGAG+AGG - chr4.2:20459634-20459653 None:intergenic 55.0%
CACTGCAAAGCCACCCACTT+GGG + chr4.2:20459957-20459976 MS.gene071401:CDS 55.0%
CCTCATTGACCACGGCTTTC+AGG - chr4.2:20460238-20460257 None:intergenic 55.0%
CCTGAAAGCCGTGGTCAATG+AGG + chr4.2:20460235-20460254 MS.gene071401:intron 55.0%
CTTTGCCTCTCAAGATCCCG+AGG + chr4.2:20459626-20459645 MS.gene071401:CDS 55.0%
GCATGTGTGATAGGTGGCAC+TGG + chr4.2:20458876-20458895 MS.gene071401:CDS 55.0%
GCCTCTCAAGATCCCGAGGT+AGG + chr4.2:20459630-20459649 MS.gene071401:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 20458840 20461294 20458840 ID=MS.gene071401
chr4.2 mRNA 20458840 20461294 20458840 ID=MS.gene071401.t1;Parent=MS.gene071401
chr4.2 exon 20458840 20458972 20458840 ID=MS.gene071401.t1.exon1;Parent=MS.gene071401.t1
chr4.2 CDS 20458840 20458972 20458840 ID=cds.MS.gene071401.t1;Parent=MS.gene071401.t1
chr4.2 exon 20459481 20459647 20459481 ID=MS.gene071401.t1.exon2;Parent=MS.gene071401.t1
chr4.2 CDS 20459481 20459647 20459481 ID=cds.MS.gene071401.t1;Parent=MS.gene071401.t1
chr4.2 exon 20459778 20459978 20459778 ID=MS.gene071401.t1.exon3;Parent=MS.gene071401.t1
chr4.2 CDS 20459778 20459978 20459778 ID=cds.MS.gene071401.t1;Parent=MS.gene071401.t1
chr4.2 exon 20460470 20460629 20460470 ID=MS.gene071401.t1.exon4;Parent=MS.gene071401.t1
chr4.2 CDS 20460470 20460629 20460470 ID=cds.MS.gene071401.t1;Parent=MS.gene071401.t1
chr4.2 exon 20460722 20460935 20460722 ID=MS.gene071401.t1.exon5;Parent=MS.gene071401.t1
chr4.2 CDS 20460722 20460935 20460722 ID=cds.MS.gene071401.t1;Parent=MS.gene071401.t1
chr4.2 exon 20461150 20461294 20461150 ID=MS.gene071401.t1.exon6;Parent=MS.gene071401.t1
chr4.2 CDS 20461150 20461294 20461150 ID=cds.MS.gene071401.t1;Parent=MS.gene071401.t1
Gene Sequence

>MS.gene071401

ATGATGGCTAGTATCAAACAAATAGAAAAGAAGAAGGCATGTGTGATAGGTGGCACTGGTTTTGTGGCATCATTGCTGATCAAGCAGTTGCTTGAAAAGGGTTATGCTGTTAATACTACTGTTAGAGACCCAGGTCTCTCTCTTAACATCTCATTGTATAATAATAATTGTCTATATATTTATGTTAATATTTTATATTATATGACAATATATCAACCAAAATATTAAAATATTGTGTGCTGCCAGTTTTTTAATCGCTAATTCATGGATAAAAATATATATTCAATTTTCACTGTAAGTAAAATTTTGTGTCATTTAGACCCAAAAAGAATTTGCATGTGTCATGTTACTATTGTTTTTACAGTTGAGTTGATAATAATATAATATATATTGTCTTAGTTTTTTGTTAGTGTTTTTTTTTCTACTTACCAAACACAATCTCTTAAGCACATGTTTTGACCGGAGAAGTGTGCCACTGTAATTTTGACCAAATTACGGTGTCACCATGATTTTGTCAAATTTATCGTCTCAAAACAAGAAGTACTAGGCCTATCTCGCTATTGAAGTAGTTTTATAGGAGAGCAGAAAATACAAATAAGATTTTGCTAAAAAAGATTTTTGATTTTTGTTGTTTTGTGCAGATAGTGCAAACAAAACATCTCACCTCATAGCACTGCAAAGTTTGGGAGAACTGAATCTATTTAAAGCAGAATTAACAGTTGAAGAAGATTTTGATGCTCCTATAGCAGGATGTGAACTTGTCTTCCAACTTGCTACACCTGTGAACTTTGCCTCTCAAGATCCCGAGGTAGGTTGATTGCTAAACAAGAAACTGCGATATAAAAATTTACAACCTAATTGAATTAATTTTTGAAGTATATGCATTTGGAAGAAAAATAATAGATTAATGATAAAATTCTTGTGCTATTACTATTCAGAATGACATGATAAAACCAGCAATCAAAGGTGTATTGAATGTCTTGAAAGCATGTGCAAGAGCAAAAGAAGTCAGAAGAGTTATCTTAACATCGTCAGCAGCTGCTGTGACTATAAACGAACTCGAAGGGACTGGTCATATTATGGATGAAACCAACTGGTCTGATGTTGAGTTTTTGAACACTGCAAAGCCACCCACTTGGGTAAAAATCAAACTTTATTTAAAATATCCAGATTATTTTGACATAAAATGGGATAACAGTTTCCGACAACAATTTAAATGGTTATGCAGTTTAAAATTGAATACATGGTTATGGTTAAGATTTCAAATAAGGTATCATGGCCTATTAACCATTCACTGAACATCATTAACCAAGATTTTGAATGTCATTCTCCACGTTTTTCACGTATATTTGAACACAGTTTGGATGATTTATGAATATCATGTGTATTTGAACACCTGAAAGCCGTGGTCAATGAGGTTTAAGATCGTGTTTAATTTTTTTCAATGGATGATTAATCATCCACTGACCATAATATATATATGAATTGCATTGACCATTCAGTTTCAAATTGTTGTCGATATTTGGTTTAAAATTTGTGTCAAAATATCATTTATGTACTCAACTTAGGTAACATTGCCCTGCCCTCATTATCATTTCTAATTGGAAAAAATTATCATCAAAATGTGCAGGGTTATCCTGTCTCAAAAGTGCTAGCTGAAAAGGCTGCATGGAAATTTGCCGAAGAAAATAACATTGATCTAATCACTGTGATACCTACTCTAACAATTGGTCCTTCTCTAACACAAGATATCCCATCTAGTGTTTCCATGGGAATGTCACTTCTAACAGGTGAAAAACAGCCAAATTAAATGGTTTAAGGATTTCCAAGGACAATACACAAAGACTCATTAATGTTTCAATCTCAATTTTATATTTTGCAGGCAATGATTTCCTCATAAATGCTTTGAAAGGAATGCAGATCCTGTCAGGTTCGATATCCATCACTCATGTCGAGGATATTTGCCGAGCTCATATATTTGTAGCAGAAAAAGAATCAGCTTCTGGTCGATATATTTGTTGTGCTCACAATACTAGTGTTCCTGAGCTTGCTAAGTTTCTCAGCAAACGATACTCTCAGTATAAAGTTCCAACTGAGTAAGCTTGAAGTCATAATTTGAAATATTTACTAAGTTTCTCATCCTCTGAAGCTGCAAAGTTCAAATATTTTCCACAGAGAACAATGATTATTTCATAGTGCTCGGTTTTCTATTTTTGAAATGACTTATTGTATACATTACCTATCTTTCAAAGGATTTTCCTTCATCAGCATTAGTTTCTCCTAAGGCAAAGTTTCATTTTTTAATTGCAGATTTGATGATTGCTCCAGCAAGGCAAAGTTGATAATCTCTTCTGAAAAGCTTATCAAAGAAGGGTTCAGTTTCAAGCATGGTATTGCTGAAACTTTTGACCAAACTCTGGAGTATTTGAAGACTCAGGGGGCCTTGAAGAAGTAG
Protein sequence

>MS.gene071401.t1

MMASIKQIEKKKACVIGGTGFVASLLIKQLLEKGYAVNTTVRDPDSANKTSHLIALQSLGELNLFKAELTVEEDFDAPIAGCELVFQLATPVNFASQDPENDMIKPAIKGVLNVLKACARAKEVRRVILTSSAAAVTINELEGTGHIMDETNWSDVEFLNTAKPPTWGYPVSKVLAEKAAWKFAEENNIDLITVIPTLTIGPSLTQDIPSSVSMGMSLLTGNDFLINALKGMQILSGSISITHVEDICRAHIFVAEKESASGRYICCAHNTSVPELAKFLSKRYSQYKVPTEFDDCSSKAKLIISSEKLIKEGFSFKHGIAETFDQTLEYLKTQGALKK