AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028567

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028567.t1 MTR_4g092080 94.940 336 15 1 1 334 1 336 0.0 654
MS.gene028567.t1 MTR_1g022440 42.249 329 179 4 12 338 8 327 3.53e-96 289
MS.gene028567.t1 MTR_1g022445 43.598 328 174 4 13 338 9 327 6.23e-95 285
MS.gene028567.t1 MTR_7g074820 40.964 332 185 6 9 336 5 329 4.10e-80 247
MS.gene028567.t1 MTR_7g074730 37.952 332 194 5 9 336 5 328 4.81e-78 242
MS.gene028567.t1 MTR_7g074880 39.157 332 188 6 9 336 5 326 1.24e-77 241
MS.gene028567.t1 MTR_7g074850 39.759 332 189 6 9 336 5 329 6.03e-77 239
MS.gene028567.t1 MTR_7g074710 37.349 332 194 5 9 336 5 326 5.39e-74 231
MS.gene028567.t1 MTR_4g081440 38.344 326 189 4 11 327 23 345 1.80e-72 229
MS.gene028567.t1 MTR_4g081440 38.690 336 173 7 11 327 19 340 3.35e-72 228
MS.gene028567.t1 MTR_2g101330 36.970 330 194 8 9 336 2 319 1.75e-65 210
MS.gene028567.t1 MTR_8g062440 39.216 306 171 5 8 311 4 296 5.22e-65 208
MS.gene028567.t1 MTR_3g005210 38.509 322 182 7 13 331 7 315 6.78e-65 208
MS.gene028567.t1 MTR_8g062440 39.871 311 171 6 8 315 4 301 8.47e-65 208
MS.gene028567.t1 MTR_4g077100 39.498 319 172 7 3 315 2 305 7.65e-64 206
MS.gene028567.t1 MTR_4g077130 39.490 314 169 7 8 315 5 303 1.76e-63 204
MS.gene028567.t1 MTR_4g077100 40.830 289 157 5 3 289 2 278 2.37e-63 202
MS.gene028567.t1 MTR_3g005170 37.654 324 186 7 13 333 11 321 4.34e-63 203
MS.gene028567.t1 MTR_3g005170 40.502 279 152 5 13 289 11 277 5.63e-63 202
MS.gene028567.t1 MTR_8g062440 39.744 312 171 7 8 315 4 302 8.07e-63 202
MS.gene028567.t1 MTR_8g062110 37.690 329 188 7 8 333 5 319 1.09e-62 202
MS.gene028567.t1 MTR_8g062110 37.920 327 186 7 8 331 14 326 1.36e-62 202
MS.gene028567.t1 MTR_8g062110 39.929 283 155 5 8 288 5 274 6.12e-62 199
MS.gene028567.t1 MTR_8g062110 39.929 283 155 5 8 288 5 274 7.69e-62 199
MS.gene028567.t1 MTR_4g009040 38.623 334 179 11 10 337 11 324 1.10e-57 189
MS.gene028567.t1 MTR_7g074870 36.299 281 163 4 60 336 20 288 2.80e-57 187
MS.gene028567.t1 MTR_4g023730 33.628 339 202 7 8 336 4 329 5.57e-56 185
MS.gene028567.t1 MTR_4g009040 38.166 338 179 12 10 337 11 328 1.16e-55 184
MS.gene028567.t1 MTR_5g056600 33.333 330 199 9 13 336 5 319 3.45e-54 180
MS.gene028567.t1 MTR_4g009690 38.138 333 181 10 10 337 11 323 2.83e-51 173
MS.gene028567.t1 MTR_2g013230 31.045 335 221 6 11 336 7 340 5.09e-51 173
MS.gene028567.t1 MTR_5g072620 33.731 335 196 9 10 336 3 319 5.35e-51 172
MS.gene028567.t1 MTR_3g005170 34.259 324 166 8 13 333 11 290 2.91e-50 169
MS.gene028567.t1 MTR_2g104960 37.920 327 182 11 13 336 21 329 1.20e-49 169
MS.gene028567.t1 MTR_4g009690 38.024 334 181 11 10 337 11 324 1.44e-49 168
MS.gene028567.t1 MTR_2g013260 31.343 335 216 7 11 334 7 338 1.21e-48 167
MS.gene028567.t1 MTR_4g006940 35.474 327 190 10 13 336 18 326 9.32e-48 164
MS.gene028567.t1 MTR_3g031650 31.940 335 198 9 10 334 3 317 2.97e-47 162
MS.gene028567.t1 MTR_4g006940 37.102 283 160 7 10 291 15 280 3.77e-47 161
MS.gene028567.t1 MTR_4g006940 37.102 283 160 7 10 291 15 280 3.77e-47 161
MS.gene028567.t1 MTR_2g013250 30.060 336 225 6 8 334 3 337 2.00e-46 160
MS.gene028567.t1 MTR_7g117190 30.973 339 217 7 11 336 6 340 3.04e-46 160
MS.gene028567.t1 MTR_2g013260 32.872 289 181 6 11 288 7 293 1.46e-44 154
MS.gene028567.t1 MTR_3g031650 32.168 286 170 6 10 288 3 271 3.19e-44 153
MS.gene028567.t1 MTR_7g117190 31.650 297 187 6 9 292 4 297 7.39e-42 148
MS.gene028567.t1 MTR_2g028620 31.845 336 201 14 13 338 11 328 1.15e-40 145
MS.gene028567.t1 MTR_2g028620 33.569 283 166 11 13 288 11 278 1.07e-39 142
MS.gene028567.t1 MTR_8g062110 34.764 233 136 6 101 331 13 231 4.09e-32 120
MS.gene028567.t1 MTR_8g062110 36.649 191 107 4 99 288 11 188 2.78e-31 117
MS.gene028567.t1 MTR_2g013230 28.033 239 163 5 106 336 13 250 1.39e-27 108
MS.gene028567.t1 MTR_0164s0070 38.411 151 89 4 9 155 2 152 2.57e-26 104
MS.gene028567.t1 MTR_2g013260 28.452 239 158 6 106 334 32 267 1.17e-24 101
MS.gene028567.t1 MTR_7g117190 28.017 232 151 6 117 336 3 230 9.61e-22 92.4
MS.gene028567.t1 MTR_6g406250 27.372 274 165 8 5 265 48 300 4.44e-21 93.2
MS.gene028567.t1 MTR_3g011130 41.176 119 65 4 102 218 30 145 6.71e-17 77.0
MS.gene028567.t1 MTR_6g406250 26.977 215 140 5 5 210 48 254 1.02e-16 79.3
MS.gene028567.t1 MTR_0685s0030 33.553 152 85 3 168 315 3 142 4.19e-16 75.1
MS.gene028567.t1 MTR_0685s0030 35.246 122 70 1 168 289 3 115 1.92e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028567.t1 AT1G61720 60.976 328 128 0 10 337 11 338 1.40e-144 412
MS.gene028567.t1 AT5G42800 42.296 331 180 4 8 336 4 325 1.73e-95 288
MS.gene028567.t1 AT4G35420 40.729 329 182 7 9 336 5 321 1.37e-75 236
MS.gene028567.t1 AT4G27250 38.110 328 188 7 13 328 14 338 3.39e-66 213
MS.gene028567.t1 AT1G51410 37.092 337 188 9 8 337 5 324 3.27e-64 206
MS.gene028567.t1 AT2G45400 35.952 331 198 6 13 336 41 364 1.50e-62 203
MS.gene028567.t1 AT4G27250 38.110 328 178 10 13 328 14 328 2.93e-62 202
MS.gene028567.t1 AT1G09510 41.667 276 147 5 10 283 6 269 6.28e-62 200
MS.gene028567.t1 AT5G19440 36.667 330 193 7 8 334 6 322 2.01e-59 194
MS.gene028567.t1 AT1G09490 40.071 282 153 6 10 288 6 274 1.08e-58 192
MS.gene028567.t1 AT1G66800 36.310 336 186 9 8 336 4 318 2.84e-56 186
MS.gene028567.t1 AT2G45400 37.324 284 165 5 13 290 41 317 1.04e-55 184
MS.gene028567.t1 AT1G09480 40.000 275 149 6 10 281 6 267 1.16e-55 184
MS.gene028567.t1 AT1G09480 40.000 275 149 6 10 281 53 314 2.15e-55 185
MS.gene028567.t1 AT2G33590 36.391 327 191 9 4 328 1 312 2.34e-55 183
MS.gene028567.t1 AT2G33600 37.654 324 185 9 7 328 4 312 5.00e-55 182
MS.gene028567.t1 AT1G09500 39.007 282 147 7 10 283 6 270 5.73e-55 181
MS.gene028567.t1 AT1G09500 41.445 263 129 7 10 264 6 251 2.43e-54 181
MS.gene028567.t1 AT1G68540 32.326 331 201 11 14 336 6 321 5.77e-49 167
MS.gene028567.t1 AT2G02400 31.765 340 200 12 7 336 1 318 1.70e-48 166
MS.gene028567.t1 AT2G45400 33.562 292 182 5 50 336 23 307 2.23e-48 165
MS.gene028567.t1 AT5G58490 33.645 321 185 9 8 312 5 313 1.69e-47 163
MS.gene028567.t1 AT1G76470 35.329 334 199 8 9 338 4 324 1.20e-46 161
MS.gene028567.t1 AT1G15950 38.462 286 158 8 10 294 11 279 1.25e-46 160
MS.gene028567.t1 AT1G25460 32.012 328 204 8 14 336 6 319 2.76e-46 160
MS.gene028567.t1 AT1G15950 36.061 330 190 11 10 336 11 322 1.66e-45 158
MS.gene028567.t1 AT1G15950 36.061 330 190 11 10 336 11 322 1.66e-45 158
MS.gene028567.t1 AT1G80820 35.435 333 194 11 7 336 3 317 5.24e-45 157
MS.gene028567.t1 AT1G15950 36.164 318 183 10 10 324 11 311 1.79e-44 155
MS.gene028567.t1 AT1G15950 37.809 283 158 8 10 291 11 276 3.18e-44 153
MS.gene028567.t1 AT1G15950 37.943 282 157 8 10 290 11 275 5.97e-44 152
MS.gene028567.t1 AT1G09490 38.525 244 134 6 48 288 13 243 1.73e-43 152
MS.gene028567.t1 AT1G80820 37.413 286 161 8 7 291 3 271 1.75e-43 151
MS.gene028567.t1 AT1G09500 40.000 230 113 7 43 264 5 217 2.27e-40 144
MS.gene028567.t1 AT2G45400 39.070 215 125 4 13 221 41 255 1.60e-39 140
MS.gene028567.t1 AT2G23910 27.344 256 166 8 12 265 10 247 2.01e-25 103
MS.gene028567.t1 AT2G23910 27.344 256 166 8 12 265 10 247 2.17e-25 103
MS.gene028567.t1 AT2G23910 27.344 256 166 8 12 265 10 247 2.18e-25 103
MS.gene028567.t1 AT4G30470 25.391 256 170 7 12 265 10 246 5.96e-24 100
MS.gene028567.t1 AT1G15950 32.489 237 145 7 102 336 1 224 2.91e-23 97.1
MS.gene028567.t1 AT1G68540 27.193 228 147 8 114 336 1 214 1.93e-22 94.0
MS.gene028567.t1 AT2G23910 32.075 159 94 4 107 265 69 213 6.99e-20 88.2
MS.gene028567.t1 AT5G14700 28.195 266 162 10 13 265 56 305 2.50e-19 88.2
MS.gene028567.t1 AT5G14700 30.151 199 128 7 13 203 56 251 1.19e-16 79.3
MS.gene028567.t1 AT4G33360 27.985 268 164 9 6 271 9 249 2.28e-15 75.9
MS.gene028567.t1 AT4G33360 27.985 268 164 9 6 271 9 249 3.09e-15 76.3
MS.gene028567.t1 AT4G33360 28.137 263 160 9 11 271 2 237 1.37e-14 73.9

Find 54 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATCAACTTTGCCTTGC+TGG 0.226247 4.3:-22220948 None:intergenic
CCTCATAGCACTGCAAAGTT+TGG 0.275725 4.3:+22219283 MS.gene028567:CDS
CGAAGGGACTGGTCATATTA+TGG 0.276006 4.3:+22219680 MS.gene028567:CDS
TTCAATACACCTTTGATTGC+TGG 0.333253 4.3:-22219573 None:intergenic
TCTTCAAATACTCCAGAGTT+TGG 0.365279 4.3:-22221033 None:intergenic
TTCCTTTCAAAGCATTTATG+AGG 0.388148 4.3:-22220516 None:intergenic
ATTCCCATGGAAACACTAGA+TGG 0.394524 4.3:-22220377 None:intergenic
TCTGAAAAGCTTATCAAAGA+AGG 0.411453 4.3:+22220974 MS.gene028567:CDS
AAGAAGAAGGCATGTGTGAT+AGG 0.414338 4.3:+22218644 MS.gene028567:CDS
CTCATAGCACTGCAAAGTTT+GGG 0.415377 4.3:+22219284 MS.gene028567:CDS
TATCAAAATCTACTTCTTCA+AGG 0.417228 4.3:-22221065 None:intergenic
ATGGGAATGTCACTTCTAAC+AGG 0.424075 4.3:+22220392 MS.gene028567:CDS
CTCAAAAGTGCTAGCTGAAA+AGG 0.425883 4.3:+22220265 MS.gene028567:CDS
GTGATACCTACTCTAACAAT+TGG 0.428631 4.3:+22220332 MS.gene028567:CDS
ACAAGTTCACATCCTGCTAT+AGG 0.437816 4.3:-22219359 None:intergenic
GATCAAGCAGTTGCTTGAAA+AGG 0.456606 4.3:+22218694 MS.gene028567:CDS
AAAGGAATGCAGATCCTGTC+AGG 0.463412 4.3:+22220532 MS.gene028567:CDS
CTGAGAAACTTAGCAAGCTC+AGG 0.468414 4.3:-22220664 None:intergenic
ATCAAGCAGTTGCTTGAAAA+GGG 0.470848 4.3:+22218695 MS.gene028567:CDS
TATCCCATCTAGTGTTTCCA+TGG 0.473150 4.3:+22220373 MS.gene028567:CDS
TTCCTCATAAATGCTTTGAA+AGG 0.475020 4.3:+22220514 MS.gene028567:CDS
TGACTTCAAGCTTACTCAGT+TGG 0.478141 4.3:-22220712 None:intergenic
CCAAACTTTGCAGTGCTATG+AGG 0.494447 4.3:-22219283 None:intergenic
ATATTATGGATGAAACCAAC+TGG 0.503991 4.3:+22219694 MS.gene028567:CDS
CTGAAAAGCTTATCAAAGAA+GGG 0.506098 4.3:+22220975 MS.gene028567:CDS
TCTTGAGAGGCAAAGTTCAC+AGG 0.513934 4.3:-22219398 None:intergenic
TCTGGAGTATTTGAAGACTC+AGG 0.526497 4.3:+22221039 MS.gene028567:CDS
CTGCTACAAATATATGAGCT+CGG 0.536593 4.3:-22220588 None:intergenic
TGCTAGCTGAAAAGGCTGCA+TGG 0.537823 4.3:+22220273 MS.gene028567:CDS
TGATGGATATCGAACCTGAC+AGG 0.542607 4.3:-22220546 None:intergenic
CTGGAGTATTTGAAGACTCA+GGG 0.551188 4.3:+22221040 MS.gene028567:CDS
AGTTCACAGGTGTAGCAAGT+TGG 0.553446 4.3:-22219385 None:intergenic
GAAGGACCAATTGTTAGAGT+AGG 0.556874 4.3:-22220338 None:intergenic
CAAACAAATAGAAAAGAAGA+AGG 0.557146 4.3:+22218631 MS.gene028567:CDS
AAACTCAACATCAGACCAGT+TGG 0.559402 4.3:-22219709 None:intergenic
GCATGTGTGATAGGTGGCAC+TGG 0.564473 4.3:+22218653 MS.gene028567:CDS
ATGATAAAACCAGCAATCAA+AGG 0.568131 4.3:+22219564 MS.gene028567:CDS
AATATCCTCAACATGAGTGA+TGG 0.569390 4.3:-22220563 None:intergenic
ATAAACGAACTCGAAGGGAC+TGG 0.592956 4.3:+22219669 MS.gene028567:CDS
ATTTGATGATTGCTCCAGCA+AGG 0.593062 4.3:+22220934 MS.gene028567:CDS
ATCCCATCTAGTGTTTCCAT+GGG 0.594221 4.3:+22220374 MS.gene028567:CDS
GGGATATCTTGTGTTAGAGA+AGG 0.600247 4.3:-22220356 None:intergenic
GAAGGGTTCAGTTTCAAGCA+TGG 0.601008 4.3:+22220992 MS.gene028567:CDS
AATACTACTGTTAGAGACCC+AGG 0.604643 4.3:+22218728 MS.gene028567:CDS
ACCTACCTCGGGATCTTGAG+AGG 0.609339 4.3:-22219411 None:intergenic
GATATCCATCACTCATGTTG+AGG 0.613457 4.3:+22220558 MS.gene028567:CDS
TTCCCATGGAAACACTAGAT+GGG 0.620353 4.3:-22220376 None:intergenic
GTGACTATAAACGAACTCGA+AGG 0.630121 4.3:+22219663 MS.gene028567:CDS
TGACTATAAACGAACTCGAA+GGG 0.632588 4.3:+22219664 MS.gene028567:CDS
CTTTGCCTCTCAAGATCCCG+AGG 0.635384 4.3:+22219406 MS.gene028567:CDS
TGTTAGAAGTGACATTCCCA+TGG 0.640999 4.3:-22220390 None:intergenic
AAGAAGGCATGTGTGATAGG+TGG 0.652368 4.3:+22218647 MS.gene028567:CDS
GGAGTATTTGAAGACTCAGG+GGG 0.662530 4.3:+22221042 MS.gene028567:CDS
TGGAGTATTTGAAGACTCAG+GGG 0.686930 4.3:+22221041 MS.gene028567:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTCAAAAATTAATTCAATT+AGG - chr4.3:22219476-22219495 None:intergenic 15.0%
!!! AGATTATTTTGACATAAAAT+GGG + chr4.3:22219788-22219807 MS.gene028567:intron 15.0%
!!! CACACAATATTTTAATATTT+TGG - chr4.3:22218836-22218855 None:intergenic 15.0%
!! AATCCTTAAACCATTTAATT+TGG - chr4.3:22220428-22220447 None:intergenic 20.0%
!! ATAACCATTTAAATTGTTGT+CGG - chr4.3:22219824-22219843 None:intergenic 20.0%
!! TAAAATTGAATACATGGTTA+TGG + chr4.3:22219850-22219869 MS.gene028567:intron 20.0%
!! TATATATATATTATGGTCAG+CGG - chr4.3:22220083-22220102 None:intergenic 20.0%
!! TATGGTTAAGATTTCAAATA+AGG + chr4.3:22219868-22219887 MS.gene028567:intron 20.0%
!!! ATCTCAATTTTATATTTTGC+AGG + chr4.3:22220484-22220503 MS.gene028567:intron 20.0%
!!! ATTTTTGAAGTATATGCATT+TGG + chr4.3:22219486-22219505 MS.gene028567:intron 20.0%
!!! CAGATTATTTTGACATAAAA+TGG + chr4.3:22219787-22219806 MS.gene028567:intron 20.0%
!!! CATTTTATGTCAAAATAATC+TGG - chr4.3:22219789-22219808 None:intergenic 20.0%
!!! CTGTAATTTTGACAAAATTA+CGG + chr4.3:22219095-22219114 MS.gene028567:intron 20.0%
!!! TAATTTTGTCAAAATTACAG+TGG - chr4.3:22219095-22219114 None:intergenic 20.0%
!!! TAGATCAATGTTATTTTCTT+CGG - chr4.3:22220306-22220325 None:intergenic 20.0%
!!! TCGTGTTTAATTTTTTTCAA+TGG + chr4.3:22220045-22220064 MS.gene028567:intron 20.0%
! AAAAAATGAAACTTTGCCTT+AGG - chr4.3:22220906-22220925 None:intergenic 25.0%
! AAATTATCATCAAAATGTGC+AGG + chr4.3:22220232-22220251 MS.gene028567:intron 25.0%
! AATGATTATTTCATAGTGCT+CGG + chr4.3:22220804-22220823 MS.gene028567:intron 25.0%
! AATTATCATCAAAATGTGCA+GGG + chr4.3:22220233-22220252 MS.gene028567:intron 25.0%
! ATCATTTATGTACTCAACTT+AGG + chr4.3:22220170-22220189 MS.gene028567:intron 25.0%
! CAAACAAATAGAAAAGAAGA+AGG + chr4.3:22218631-22218650 MS.gene028567:CDS 25.0%
! CGATAAATTTGACAAAATCA+TGG - chr4.3:22219126-22219145 None:intergenic 25.0%
! TGAAAAACAGCCAAATTAAA+TGG + chr4.3:22220415-22220434 MS.gene028567:intron 25.0%
! TGAATTAGCGATTAAAAAAC+TGG - chr4.3:22218862-22218881 None:intergenic 25.0%
! TTCAAATTGTTGTCGATATT+TGG + chr4.3:22220127-22220146 MS.gene028567:intron 25.0%
!! AGATTTCAAATAAGGTATCA+TGG + chr4.3:22219876-22219895 MS.gene028567:intron 25.0%
!! CCAATTAGAAATGATAATGA+GGG - chr4.3:22220209-22220228 None:intergenic 25.0%
!! GCAGTTTAAAATTGAATACA+TGG + chr4.3:22219844-22219863 MS.gene028567:intron 25.0%
!! TCCAATTAGAAATGATAATG+AGG - chr4.3:22220210-22220229 None:intergenic 25.0%
!!! GTTTTTTAATCGCTAATTCA+TGG + chr4.3:22218862-22218881 MS.gene028567:intron 25.0%
AAATGGTTTAAGGATTTCCA+AGG + chr4.3:22220432-22220451 MS.gene028567:intron 30.0%
ACGTATATTTGAACACAGTT+TGG + chr4.3:22219961-22219980 MS.gene028567:intron 30.0%
ATATTATGGATGAAACCAAC+TGG + chr4.3:22219694-22219713 MS.gene028567:CDS 30.0%
ATGATAAAACCAGCAATCAA+AGG + chr4.3:22219564-22219583 MS.gene028567:CDS 30.0%
ATGTTCAGTGAATGGTTAAT+AGG - chr4.3:22219902-22219921 None:intergenic 30.0%
ATTCATTACCTATCTTTCAG+AGG + chr4.3:22220854-22220873 MS.gene028567:intron 30.0%
CAGCCAAATTAAATGGTTTA+AGG + chr4.3:22220422-22220441 MS.gene028567:intron 30.0%
CCCTCATTATCATTTCTAAT+TGG + chr4.3:22220206-22220225 MS.gene028567:intron 30.0%
CTGAAAAGCTTATCAAAGAA+GGG + chr4.3:22220975-22220994 MS.gene028567:CDS 30.0%
GAAATAATCATTGTTCTCTG+TGG - chr4.3:22220796-22220815 None:intergenic 30.0%
GAGAATGACATTCAAAATCT+TGG - chr4.3:22219931-22219950 None:intergenic 30.0%
GTTTCCGACAACAATTTAAA+TGG + chr4.3:22219817-22219836 MS.gene028567:intron 30.0%
TCAAATATACGTGAAAAACG+TGG - chr4.3:22219953-22219972 None:intergenic 30.0%
TCTGAAAAGCTTATCAAAGA+AGG + chr4.3:22220974-22220993 MS.gene028567:CDS 30.0%
! GACAACAATTTGAAACTGAA+TGG - chr4.3:22220121-22220140 None:intergenic 30.0%
! GACACATGCAATTTCTTTTT+GGG - chr4.3:22218940-22218959 None:intergenic 30.0%
! TGACACATGCAATTTCTTTT+TGG - chr4.3:22218941-22218960 None:intergenic 30.0%
! TTCCTCATAAATGCTTTGAA+AGG + chr4.3:22220514-22220533 MS.gene028567:CDS 30.0%
! TTCCTTTCAAAGCATTTATG+AGG - chr4.3:22220519-22220538 None:intergenic 30.0%
!! AAGTTTGATTTTTACCCAAG+TGG - chr4.3:22219754-22219773 None:intergenic 30.0%
!! AGTTTGATTTTTACCCAAGT+GGG - chr4.3:22219753-22219772 None:intergenic 30.0%
!!! CGCTATTGAAGTAGTTTTAT+TGG + chr4.3:22219175-22219194 MS.gene028567:intron 30.0%
AACTACTTCAATAGCGAGAT+AGG - chr4.3:22219171-22219190 None:intergenic 35.0%
AATATCCTCAACATGAGTGA+TGG - chr4.3:22220566-22220585 None:intergenic 35.0%
ATCAAGCAGTTGCTTGAAAA+GGG + chr4.3:22218695-22218714 MS.gene028567:CDS 35.0%
CTGCTACAAATATATGAGCT+CGG - chr4.3:22220591-22220610 None:intergenic 35.0%
GGTTAATGATGTTCAGTGAA+TGG - chr4.3:22219910-22219929 None:intergenic 35.0%
GTGATACCTACTCTAACAAT+TGG + chr4.3:22220332-22220351 MS.gene028567:CDS 35.0%
TCTTCAAATACTCCAGAGTT+TGG - chr4.3:22221036-22221055 None:intergenic 35.0%
TGACTATAAACGAACTCGAA+GGG + chr4.3:22219664-22219683 MS.gene028567:CDS 35.0%
TGTGCTTAAGAGATTGTGTT+TGG - chr4.3:22219051-22219070 None:intergenic 35.0%
TTCAATACACCTTTGATTGC+TGG - chr4.3:22219576-22219595 None:intergenic 35.0%
! AGGAAAATCCTCTGAAAGAT+AGG - chr4.3:22220865-22220884 None:intergenic 35.0%
! ATCAGCATTAGTTTCTCCTA+AGG + chr4.3:22220887-22220906 MS.gene028567:intron 35.0%
! TAGAAATGATAATGAGGGCA+GGG - chr4.3:22220204-22220223 None:intergenic 35.0%
! TGAAACTTTTGACCAAACTC+TGG + chr4.3:22221021-22221040 MS.gene028567:CDS 35.0%
! TTAGAAATGATAATGAGGGC+AGG - chr4.3:22220205-22220224 None:intergenic 35.0%
!! GTCTCAAAACAAGAAGTACT+AGG + chr4.3:22219145-22219164 MS.gene028567:intron 35.0%
!! GTTTGATTTTTACCCAAGTG+GGG - chr4.3:22219752-22219771 None:intergenic 35.0%
!! TTTGATTTTTACCCAAGTGG+GGG - chr4.3:22219751-22219770 None:intergenic 35.0%
!!! TCTTAAGCACATGTTTTGAC+CGG + chr4.3:22219060-22219079 MS.gene028567:intron 35.0%
AAACTCAACATCAGACCAGT+TGG - chr4.3:22219712-22219731 None:intergenic 40.0%
AATACTACTGTTAGAGACCC+AGG + chr4.3:22218728-22218747 MS.gene028567:CDS 40.0%
ACAAGTTCACATCCTGCTAT+AGG - chr4.3:22219362-22219381 None:intergenic 40.0%
AGATGTTAAGAGAGAGACCT+GGG - chr4.3:22218748-22218767 None:intergenic 40.0%
ATCCCATCTAGTGTTTCCAT+GGG + chr4.3:22220374-22220393 MS.gene028567:CDS 40.0%
ATTCCCATGGAAACACTAGA+TGG - chr4.3:22220380-22220399 None:intergenic 40.0%
ATTTGAACACCTGAAAGTCG+TGG + chr4.3:22220006-22220025 MS.gene028567:intron 40.0%
CTCATAGCACTGCAAAGTTT+GGG + chr4.3:22219284-22219303 MS.gene028567:CDS 40.0%
GAAGGACCAATTGTTAGAGT+AGG - chr4.3:22220341-22220360 None:intergenic 40.0%
GAGTCTTTGTGTATTGTCCT+TGG - chr4.3:22220452-22220471 None:intergenic 40.0%
GATATCCATCACTCATGTTG+AGG + chr4.3:22220558-22220577 MS.gene028567:CDS 40.0%
GATCAAGCAGTTGCTTGAAA+AGG + chr4.3:22218694-22218713 MS.gene028567:CDS 40.0%
GATTATCAACTTTGCCTTGC+TGG - chr4.3:22220951-22220970 None:intergenic 40.0%
GCAGAAAAAGAATCAGCTTC+TGG + chr4.3:22220607-22220626 MS.gene028567:CDS 40.0%
GGAGAAACTAATGCTGATGA+AGG - chr4.3:22220885-22220904 None:intergenic 40.0%
GGGATATCTTGTGTTAGAGA+AGG - chr4.3:22220359-22220378 None:intergenic 40.0%
GTGACTATAAACGAACTCGA+AGG + chr4.3:22219663-22219682 MS.gene028567:CDS 40.0%
GTTTAGCAATCAACCTACCT+CGG - chr4.3:22219426-22219445 None:intergenic 40.0%
TATCCCATCTAGTGTTTCCA+TGG + chr4.3:22220373-22220392 MS.gene028567:CDS 40.0%
TGACTTCAAGCTTACTCAGT+TGG - chr4.3:22220715-22220734 None:intergenic 40.0%
TGTTAGAAGTGACATTCCCA+TGG - chr4.3:22220393-22220412 None:intergenic 40.0%
TTCCCATGGAAACACTAGAT+GGG - chr4.3:22220379-22220398 None:intergenic 40.0%
TTTAGCAATCAACCTACCTC+GGG - chr4.3:22219425-22219444 None:intergenic 40.0%
TTTGAACTTTGCAGCTTCAG+AGG - chr4.3:22220767-22220786 None:intergenic 40.0%
! AAGAAGAAGGCATGTGTGAT+AGG + chr4.3:22218644-22218663 MS.gene028567:CDS 40.0%
! ATGGGAATGTCACTTCTAAC+AGG + chr4.3:22220392-22220411 MS.gene028567:CDS 40.0%
! ATTTGATGATTGCTCCAGCA+AGG + chr4.3:22220934-22220953 MS.gene028567:CDS 40.0%
! CTGGAGTATTTGAAGACTCA+GGG + chr4.3:22221040-22221059 MS.gene028567:CDS 40.0%
! TCTGGAGTATTTGAAGACTC+AGG + chr4.3:22221039-22221058 MS.gene028567:CDS 40.0%
! TGGAGTATTTGAAGACTCAG+GGG + chr4.3:22221041-22221060 MS.gene028567:CDS 40.0%
! TTGATTTTTACCCAAGTGGG+GGG - chr4.3:22219750-22219769 None:intergenic 40.0%
!! CTCAAAAGTGCTAGCTGAAA+AGG + chr4.3:22220265-22220284 MS.gene028567:CDS 40.0%
!! GATTTTGATGCTCCTATAGC+AGG + chr4.3:22219347-22219366 MS.gene028567:CDS 40.0%
AAAGGAATGCAGATCCTGTC+AGG + chr4.3:22220532-22220551 MS.gene028567:CDS 45.0%
AGTTCACAGGTGTAGCAAGT+TGG - chr4.3:22219388-22219407 None:intergenic 45.0%
ATAAACGAACTCGAAGGGAC+TGG + chr4.3:22219669-22219688 MS.gene028567:CDS 45.0%
ATTACAGTGGCACACTTCTC+CGG - chr4.3:22219082-22219101 None:intergenic 45.0%
CCAAACTTTGCAGTGCTATG+AGG - chr4.3:22219286-22219305 None:intergenic 45.0%
CCTCATAGCACTGCAAAGTT+TGG + chr4.3:22219283-22219302 MS.gene028567:CDS 45.0%
CTGAGAAACTTAGCAAGCTC+AGG - chr4.3:22220667-22220686 None:intergenic 45.0%
GAAGGGTTCAGTTTCAAGCA+TGG + chr4.3:22220992-22221011 MS.gene028567:CDS 45.0%
GAGATGTTAAGAGAGAGACC+TGG - chr4.3:22218749-22218768 None:intergenic 45.0%
TCTTGAGAGGCAAAGTTCAC+AGG - chr4.3:22219401-22219420 None:intergenic 45.0%
! AAGAAGGCATGTGTGATAGG+TGG + chr4.3:22218647-22218666 MS.gene028567:CDS 45.0%
! GGAGTATTTGAAGACTCAGG+GGG + chr4.3:22221042-22221061 MS.gene028567:CDS 45.0%
! TGATGGATATCGAACCTGAC+AGG - chr4.3:22220549-22220568 None:intergenic 45.0%
!! CGAAGGGACTGGTCATATTA+TGG + chr4.3:22219680-22219699 MS.gene028567:CDS 45.0%
!!! TCAGCTAGCACTTTTGAGAC+AGG - chr4.3:22220263-22220282 None:intergenic 45.0%
!! ATTCATATATATATATATTA+TGG - chr4.3:22220090-22220109 None:intergenic 5.0%
CCTCATTGACCACGACTTTC+AGG - chr4.3:22220018-22220037 None:intergenic 50.0%
CCTGAAAGTCGTGGTCAATG+AGG + chr4.3:22220015-22220034 MS.gene028567:intron 50.0%
TGCTAGCTGAAAAGGCTGCA+TGG + chr4.3:22220273-22220292 MS.gene028567:CDS 50.0%
!!! GATAGGTGGCACTGGTTTTG+TGG + chr4.3:22218661-22218680 MS.gene028567:CDS 50.0%
ACCTACCTCGGGATCTTGAG+AGG - chr4.3:22219414-22219433 None:intergenic 55.0%
CTTTGCCTCTCAAGATCCCG+AGG + chr4.3:22219406-22219425 MS.gene028567:CDS 55.0%
GCATGTGTGATAGGTGGCAC+TGG + chr4.3:22218653-22218672 MS.gene028567:CDS 55.0%
ACACTGCAAAGCCCCCCACT+TGG + chr4.3:22219736-22219755 MS.gene028567:CDS 60.0%
CACTGCAAAGCCCCCCACTT+GGG + chr4.3:22219737-22219756 MS.gene028567:CDS 60.0%
GCCTCTCAAGATCCCGAGGT+AGG + chr4.3:22219410-22219429 MS.gene028567:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 22218620 22221078 22218620 ID=MS.gene028567
chr4.3 mRNA 22218620 22221078 22218620 ID=MS.gene028567.t1;Parent=MS.gene028567
chr4.3 exon 22218620 22218749 22218620 ID=MS.gene028567.t1.exon1;Parent=MS.gene028567.t1
chr4.3 CDS 22218620 22218749 22218620 ID=cds.MS.gene028567.t1;Parent=MS.gene028567.t1
chr4.3 exon 22219261 22219427 22219261 ID=MS.gene028567.t1.exon2;Parent=MS.gene028567.t1
chr4.3 CDS 22219261 22219427 22219261 ID=cds.MS.gene028567.t1;Parent=MS.gene028567.t1
chr4.3 exon 22219558 22219758 22219558 ID=MS.gene028567.t1.exon3;Parent=MS.gene028567.t1
chr4.3 CDS 22219558 22219758 22219558 ID=cds.MS.gene028567.t1;Parent=MS.gene028567.t1
chr4.3 exon 22220254 22220413 22220254 ID=MS.gene028567.t1.exon4;Parent=MS.gene028567.t1
chr4.3 CDS 22220254 22220413 22220254 ID=cds.MS.gene028567.t1;Parent=MS.gene028567.t1
chr4.3 exon 22220506 22220719 22220506 ID=MS.gene028567.t1.exon5;Parent=MS.gene028567.t1
chr4.3 CDS 22220506 22220719 22220506 ID=cds.MS.gene028567.t1;Parent=MS.gene028567.t1
chr4.3 exon 22220934 22221078 22220934 ID=MS.gene028567.t1.exon6;Parent=MS.gene028567.t1
chr4.3 CDS 22220934 22221078 22220934 ID=cds.MS.gene028567.t1;Parent=MS.gene028567.t1
Gene Sequence

>MS.gene028567

ATGGCTAGTATCAAACAAATAGAAAAGAAGAAGGCATGTGTGATAGGTGGCACTGGTTTTGTGGCATCATTGCTGATCAAGCAGTTGCTTGAAAAGGGTTATGCTGTTAATACTACTGTTAGAGACCCAGGTCTCTCTCTTAACATCTCATTGTATAATAATAATTGTCTATATATTTATGTTAATATTTTATATTATATGACAATATATCAACCAAAATATTAAAATATTGTGTGCTGCCAGTTTTTTAATCGCTAATTCATGGATAAAAATATATATTCAATTTTCACTGTAAGTAAAATTTTGTGTCATTTAGACCCAAAAAGAAATTGCATGTGTCATGTTACTATTGTTTTTACAGTTGAGTTGATAATAATATAATATATATTGTCTTAGTTTTTTGTTAGTGTTTTTTTTTTTTCTACTTACCAAACACAATCTCTTAAGCACATGTTTTGACCGGAGAAGTGTGCCACTGTAATTTTGACAAAATTACGGTGTCACCATGATTTTGTCAAATTTATCGTCTCAAAACAAGAAGTACTAGGCCTATCTCGCTATTGAAGTAGTTTTATTGGAGAGCAGAAAATACAAATAAGATTTTGCTAAAAAAGATTTTTGATTTTTGTTGTTTTGTGCAGATAGTGCAAACAAAACATCTCACCTCATAGCACTGCAAAGTTTGGGAGAACTGAATCTATTTAAAGCAGAATTAACAGTTGAAGAAGATTTTGATGCTCCTATAGCAGGATGTGAACTTGTCTTCCAACTTGCTACACCTGTGAACTTTGCCTCTCAAGATCCCGAGGTAGGTTGATTGCTAAACAAGAAACTGCGATATAAAAATTTACAACCTAATTGAATTAATTTTTGAAGTATATGCATTTGGAAGAAAAATAATAGATTAATGATAAAATTCTTGTGCTATTACTATTCAGAATGACATGATAAAACCAGCAATCAAAGGTGTATTGAATGTCTTGAAAGCATGTGCAAGAGCAAAAGAAGTCAGAAGAGTTATCTTAACATCTTCAGCAGCTGCTGTGACTATAAACGAACTCGAAGGGACTGGTCATATTATGGATGAAACCAACTGGTCTGATGTTGAGTTTTTGAACACTGCAAAGCCCCCCACTTGGGTAAAAATCAAACTTTATTTAAAATATCCAGATTATTTTGACATAAAATGGGATAACAGTTTCCGACAACAATTTAAATGGTTATGCAGTTTAAAATTGAATACATGGTTATGGTTAAGATTTCAAATAAGGTATCATGGCCTATTAACCATTCACTGAACATCATTAACCAAGATTTTGAATGTCATTCTCCACGTTTTTCACGTATATTTGAACACAGTTTGGATGATTTATGAATATCATGTGTATTTGAACACCTGAAAGTCGTGGTCAATGAGGTTTAAGATCGTGTTTAATTTTTTTCAATGGATGATTAATCATCCGCTGACCATAATATATATATATATGAATTGCATTGACCATTCAGTTTCAAATTGTTGTCGATATTTGGTTTAAAATTTGTGTCAAAATATCATTTATGTACTCAACTTAGGTAACATTGCCCTGCCCTCATTATCATTTCTAATTGGAAAAAATTATCATCAAAATGTGCAGGGTTATCCTGTCTCAAAAGTGCTAGCTGAAAAGGCTGCATGGAAATTTGCCGAAGAAAATAACATTGATCTAATCACTGTGATACCTACTCTAACAATTGGTCCTTCTCTAACACAAGATATCCCATCTAGTGTTTCCATGGGAATGTCACTTCTAACAGGTGAAAAACAGCCAAATTAAATGGTTTAAGGATTTCCAAGGACAATACACAAAGACTCATTAATGTTTCAATCTCAATTTTATATTTTGCAGGCAATGATTTCCTCATAAATGCTTTGAAAGGAATGCAGATCCTGTCAGGTTCGATATCCATCACTCATGTTGAGGATATTTGCCGAGCTCATATATTTGTAGCAGAAAAAGAATCAGCTTCTGGTCGATATATTTGTTGTGCTCACAATACTAGTGTTCCTGAGCTTGCTAAGTTTCTCAGCAAACGATACTCTCAGTATAAAGTTCCAACTGAGTAAGCTTGAAGTCATAATTTGAAATATTTACTAAGTTTCTCATCCTCTGAAGCTGCAAAGTTCAAATATTTTCCACAGAGAACAATGATTATTTCATAGTGCTCGGTTTTCTATTTTTGAAATGACTTATTGTATTCATTACCTATCTTTCAGAGGATTTTCCTTCATCAGCATTAGTTTCTCCTAAGGCAAAGTTTCATTTTTTAATTGCAGATTTGATGATTGCTCCAGCAAGGCAAAGTTGATAATCTCTTCTGAAAAGCTTATCAAAGAAGGGTTCAGTTTCAAGCATGGTATTGCTGAAACTTTTGACCAAACTCTGGAGTATTTGAAGACTCAGGGGGCCTTGAAGAAGTAG
Protein sequence

>MS.gene028567.t1

MASIKQIEKKKACVIGGTGFVASLLIKQLLEKGYAVNTTVRDPDSANKTSHLIALQSLGELNLFKAELTVEEDFDAPIAGCELVFQLATPVNFASQDPENDMIKPAIKGVLNVLKACARAKEVRRVILTSSAAAVTINELEGTGHIMDETNWSDVEFLNTAKPPTWGYPVSKVLAEKAAWKFAEENNIDLITVIPTLTIGPSLTQDIPSSVSMGMSLLTGNDFLINALKGMQILSGSISITHVEDICRAHIFVAEKESASGRYICCAHNTSVPELAKFLSKRYSQYKVPTEFDDCSSKAKLIISSEKLIKEGFSFKHGIAETFDQTLEYLKTQGALKK