Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016249.t1 | Q40316.1 | 98.8 | 326 | 4 | 0 | 1 | 326 | 1 | 326 | 7.80E-184 | 652.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016249.t1 | Q40316 | 98.8 | 326 | 4 | 0 | 1 | 326 | 1 | 326 | 1.9e-186 | 652.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016249.t1 | A2Q3W4 | 98.8 | 326 | 4 | 0 | 1 | 326 | 1 | 326 | 9.6e-184 | 652.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene016249 | MS.gene56554 | PPI |
| MS.gene016249 | MS.gene006771 | PPI |
| MS.gene028567 | MS.gene016249 | PPI |
| MS.gene071401 | MS.gene016249 | PPI |
| MS.gene016249 | MS.gene96808 | PPI |
| MS.gene016249 | MS.gene40154 | PPI |
| MS.gene031525 | MS.gene016249 | PPI |
| MS.gene016249 | MS.gene40148 | PPI |
| MS.gene016249 | MS.gene071398 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016249.t1 | MTR_7g074710 | 98.773 | 326 | 4 | 0 | 1 | 326 | 1 | 326 | 0.0 | 662 |
| MS.gene016249.t1 | MTR_7g074880 | 69.632 | 326 | 99 | 0 | 1 | 326 | 1 | 326 | 1.02e-174 | 487 |
| MS.gene016249.t1 | MTR_7g074850 | 67.173 | 329 | 105 | 1 | 1 | 326 | 1 | 329 | 8.76e-170 | 474 |
| MS.gene016249.t1 | MTR_7g074730 | 66.159 | 328 | 109 | 1 | 1 | 326 | 1 | 328 | 1.19e-166 | 467 |
| MS.gene016249.t1 | MTR_7g074820 | 65.957 | 329 | 109 | 1 | 1 | 326 | 1 | 329 | 7.49e-166 | 465 |
| MS.gene016249.t1 | MTR_7g074870 | 65.385 | 286 | 99 | 0 | 41 | 326 | 3 | 288 | 2.05e-140 | 399 |
| MS.gene016249.t1 | MTR_4g023730 | 48.328 | 329 | 167 | 3 | 1 | 326 | 1 | 329 | 9.73e-106 | 312 |
| MS.gene016249.t1 | MTR_1g022440 | 43.385 | 325 | 171 | 4 | 8 | 326 | 8 | 325 | 2.79e-94 | 283 |
| MS.gene016249.t1 | MTR_1g022445 | 44.000 | 325 | 169 | 4 | 8 | 326 | 8 | 325 | 4.38e-93 | 280 |
| MS.gene016249.t1 | MTR_4g092080 | 38.485 | 330 | 189 | 5 | 5 | 324 | 11 | 336 | 5.73e-75 | 234 |
| MS.gene016249.t1 | MTR_4g081440 | 35.000 | 340 | 195 | 7 | 6 | 326 | 22 | 354 | 4.64e-69 | 219 |
| MS.gene016249.t1 | MTR_4g081440 | 34.412 | 340 | 196 | 7 | 6 | 326 | 18 | 349 | 1.98e-68 | 218 |
| MS.gene016249.t1 | MTR_2g101330 | 38.957 | 326 | 183 | 8 | 7 | 326 | 4 | 319 | 4.87e-63 | 203 |
| MS.gene016249.t1 | MTR_8g062440 | 36.254 | 331 | 191 | 8 | 4 | 326 | 3 | 321 | 2.44e-59 | 193 |
| MS.gene016249.t1 | MTR_8g062440 | 35.650 | 331 | 194 | 7 | 4 | 326 | 3 | 322 | 5.33e-58 | 190 |
| MS.gene016249.t1 | MTR_8g062110 | 36.170 | 329 | 194 | 8 | 1 | 323 | 1 | 319 | 2.24e-57 | 188 |
| MS.gene016249.t1 | MTR_8g062110 | 36.170 | 329 | 194 | 8 | 1 | 323 | 10 | 328 | 2.63e-57 | 188 |
| MS.gene016249.t1 | MTR_8g062440 | 39.643 | 280 | 153 | 6 | 4 | 276 | 3 | 273 | 4.05e-57 | 187 |
| MS.gene016249.t1 | MTR_8g062110 | 38.929 | 280 | 161 | 5 | 1 | 276 | 1 | 274 | 9.89e-57 | 185 |
| MS.gene016249.t1 | MTR_4g077130 | 38.489 | 278 | 162 | 5 | 2 | 276 | 4 | 275 | 1.00e-56 | 187 |
| MS.gene016249.t1 | MTR_8g062110 | 38.929 | 280 | 161 | 5 | 1 | 276 | 1 | 274 | 1.06e-56 | 185 |
| MS.gene016249.t1 | MTR_5g056600 | 33.956 | 321 | 203 | 6 | 9 | 326 | 5 | 319 | 2.56e-54 | 180 |
| MS.gene016249.t1 | MTR_3g005210 | 36.765 | 272 | 164 | 4 | 8 | 276 | 6 | 272 | 5.74e-54 | 179 |
| MS.gene016249.t1 | MTR_2g013230 | 33.815 | 346 | 203 | 9 | 1 | 326 | 1 | 340 | 1.52e-53 | 179 |
| MS.gene016249.t1 | MTR_4g077100 | 36.429 | 280 | 169 | 5 | 1 | 276 | 3 | 277 | 1.67e-53 | 177 |
| MS.gene016249.t1 | MTR_4g077100 | 36.429 | 280 | 169 | 5 | 1 | 276 | 3 | 277 | 6.18e-53 | 177 |
| MS.gene016249.t1 | MTR_3g005170 | 37.132 | 272 | 163 | 5 | 8 | 276 | 10 | 276 | 9.41e-53 | 175 |
| MS.gene016249.t1 | MTR_3g005170 | 37.132 | 272 | 163 | 5 | 8 | 276 | 10 | 276 | 2.52e-52 | 175 |
| MS.gene016249.t1 | MTR_3g031650 | 33.851 | 322 | 203 | 7 | 8 | 326 | 5 | 319 | 3.26e-51 | 172 |
| MS.gene016249.t1 | MTR_5g072620 | 34.783 | 322 | 200 | 7 | 8 | 326 | 5 | 319 | 1.28e-48 | 166 |
| MS.gene016249.t1 | MTR_2g013250 | 31.195 | 343 | 208 | 7 | 3 | 324 | 2 | 337 | 5.91e-48 | 164 |
| MS.gene016249.t1 | MTR_3g031650 | 35.294 | 272 | 168 | 5 | 8 | 276 | 5 | 271 | 6.01e-46 | 157 |
| MS.gene016249.t1 | MTR_2g013260 | 31.792 | 346 | 206 | 11 | 1 | 324 | 1 | 338 | 8.93e-45 | 156 |
| MS.gene016249.t1 | MTR_7g117190 | 32.362 | 343 | 209 | 10 | 3 | 326 | 2 | 340 | 1.02e-44 | 156 |
| MS.gene016249.t1 | MTR_2g104960 | 34.056 | 323 | 196 | 13 | 8 | 326 | 20 | 329 | 1.16e-42 | 150 |
| MS.gene016249.t1 | MTR_4g009040 | 32.716 | 324 | 202 | 11 | 7 | 326 | 12 | 323 | 1.17e-42 | 150 |
| MS.gene016249.t1 | MTR_4g006940 | 32.515 | 326 | 205 | 11 | 4 | 326 | 13 | 326 | 1.43e-42 | 150 |
| MS.gene016249.t1 | MTR_4g009040 | 32.012 | 328 | 203 | 11 | 7 | 326 | 12 | 327 | 5.71e-41 | 145 |
| MS.gene016249.t1 | MTR_2g028620 | 32.219 | 329 | 201 | 11 | 8 | 326 | 10 | 326 | 2.34e-39 | 141 |
| MS.gene016249.t1 | MTR_4g006940 | 34.173 | 278 | 169 | 10 | 4 | 278 | 13 | 279 | 9.58e-39 | 139 |
| MS.gene016249.t1 | MTR_4g006940 | 34.173 | 278 | 169 | 10 | 4 | 278 | 13 | 279 | 9.58e-39 | 139 |
| MS.gene016249.t1 | MTR_7g117190 | 33.106 | 293 | 176 | 9 | 3 | 276 | 2 | 293 | 1.06e-38 | 139 |
| MS.gene016249.t1 | MTR_2g013260 | 32.881 | 295 | 177 | 7 | 1 | 276 | 1 | 293 | 5.53e-38 | 137 |
| MS.gene016249.t1 | MTR_4g009690 | 32.927 | 328 | 196 | 11 | 7 | 326 | 12 | 323 | 9.21e-38 | 137 |
| MS.gene016249.t1 | MTR_4g009690 | 32.817 | 323 | 202 | 10 | 7 | 326 | 12 | 322 | 1.49e-37 | 137 |
| MS.gene016249.t1 | MTR_3g005170 | 32.353 | 272 | 145 | 6 | 8 | 276 | 10 | 245 | 1.17e-36 | 133 |
| MS.gene016249.t1 | MTR_2g028620 | 33.453 | 278 | 167 | 8 | 8 | 276 | 10 | 278 | 4.21e-36 | 132 |
| MS.gene016249.t1 | MTR_2g013230 | 30.769 | 247 | 146 | 8 | 100 | 326 | 9 | 250 | 1.63e-24 | 100 |
| MS.gene016249.t1 | MTR_0164s0070 | 40.625 | 128 | 74 | 2 | 7 | 133 | 4 | 130 | 4.90e-24 | 97.8 |
| MS.gene016249.t1 | MTR_8g062110 | 32.143 | 224 | 138 | 6 | 105 | 323 | 19 | 233 | 1.78e-23 | 97.1 |
| MS.gene016249.t1 | MTR_8g062110 | 35.429 | 175 | 105 | 3 | 105 | 276 | 19 | 188 | 7.07e-23 | 94.4 |
| MS.gene016249.t1 | MTR_5g029990 | 26.866 | 268 | 172 | 6 | 8 | 270 | 12 | 260 | 7.69e-23 | 96.7 |
| MS.gene016249.t1 | MTR_6g406250 | 26.909 | 275 | 181 | 7 | 4 | 268 | 51 | 315 | 2.31e-20 | 90.9 |
| MS.gene016249.t1 | MTR_7g117190 | 31.169 | 231 | 137 | 9 | 115 | 326 | 3 | 230 | 3.31e-19 | 85.1 |
| MS.gene016249.t1 | MTR_7g074865 | 82.500 | 40 | 7 | 0 | 1 | 40 | 1 | 40 | 3.28e-18 | 78.2 |
| MS.gene016249.t1 | MTR_6g406250 | 28.910 | 211 | 135 | 5 | 4 | 206 | 51 | 254 | 3.89e-18 | 83.2 |
| MS.gene016249.t1 | MTR_3g101500 | 27.668 | 253 | 150 | 9 | 7 | 251 | 3 | 230 | 5.93e-16 | 77.4 |
| MS.gene016249.t1 | MTR_3g101500 | 27.668 | 253 | 150 | 9 | 7 | 251 | 3 | 230 | 6.29e-16 | 77.8 |
| MS.gene016249.t1 | MTR_2g013260 | 27.935 | 247 | 149 | 10 | 100 | 324 | 28 | 267 | 9.64e-16 | 76.6 |
| MS.gene016249.t1 | MTR_3g101500 | 28.421 | 190 | 116 | 6 | 7 | 196 | 3 | 172 | 2.37e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016249.t1 | AT2G45400 | 48.171 | 328 | 162 | 5 | 6 | 326 | 38 | 364 | 5.01e-103 | 306 |
| MS.gene016249.t1 | AT5G42800 | 43.675 | 332 | 174 | 5 | 1 | 326 | 1 | 325 | 8.86e-92 | 278 |
| MS.gene016249.t1 | AT2G45400 | 48.582 | 282 | 138 | 4 | 6 | 280 | 38 | 319 | 4.39e-89 | 270 |
| MS.gene016249.t1 | AT2G45400 | 47.241 | 290 | 146 | 4 | 43 | 326 | 19 | 307 | 1.27e-86 | 263 |
| MS.gene016249.t1 | AT1G61720 | 39.104 | 335 | 186 | 8 | 4 | 326 | 9 | 337 | 1.03e-77 | 241 |
| MS.gene016249.t1 | AT4G35420 | 41.818 | 330 | 179 | 8 | 1 | 326 | 1 | 321 | 8.29e-75 | 233 |
| MS.gene016249.t1 | AT2G45400 | 50.691 | 217 | 101 | 3 | 6 | 216 | 38 | 254 | 2.43e-69 | 217 |
| MS.gene016249.t1 | AT1G09510 | 37.730 | 326 | 193 | 6 | 1 | 323 | 1 | 319 | 9.06e-57 | 187 |
| MS.gene016249.t1 | AT2G02400 | 36.086 | 327 | 195 | 8 | 4 | 326 | 2 | 318 | 2.58e-56 | 185 |
| MS.gene016249.t1 | AT4G27250 | 31.875 | 320 | 201 | 5 | 9 | 317 | 14 | 327 | 1.39e-55 | 184 |
| MS.gene016249.t1 | AT1G09480 | 36.778 | 329 | 198 | 7 | 1 | 326 | 1 | 322 | 4.32e-54 | 180 |
| MS.gene016249.t1 | AT4G27250 | 31.098 | 328 | 203 | 5 | 9 | 317 | 14 | 337 | 4.41e-54 | 181 |
| MS.gene016249.t1 | AT1G51410 | 34.242 | 330 | 203 | 7 | 2 | 326 | 3 | 323 | 6.24e-54 | 179 |
| MS.gene016249.t1 | AT5G19440 | 35.061 | 328 | 198 | 7 | 4 | 325 | 5 | 323 | 1.05e-53 | 179 |
| MS.gene016249.t1 | AT1G09480 | 36.778 | 329 | 198 | 7 | 1 | 326 | 48 | 369 | 1.10e-53 | 180 |
| MS.gene016249.t1 | AT1G09490 | 36.170 | 329 | 200 | 7 | 1 | 326 | 1 | 322 | 1.11e-53 | 179 |
| MS.gene016249.t1 | AT1G66800 | 35.185 | 324 | 201 | 8 | 4 | 326 | 3 | 318 | 1.24e-53 | 178 |
| MS.gene016249.t1 | AT1G68540 | 34.472 | 322 | 200 | 7 | 10 | 326 | 6 | 321 | 3.87e-51 | 172 |
| MS.gene016249.t1 | AT5G58490 | 34.985 | 323 | 198 | 8 | 8 | 326 | 9 | 323 | 8.28e-51 | 171 |
| MS.gene016249.t1 | AT1G09500 | 36.786 | 280 | 168 | 5 | 1 | 276 | 1 | 275 | 1.45e-49 | 167 |
| MS.gene016249.t1 | AT1G25460 | 34.579 | 321 | 199 | 6 | 10 | 326 | 6 | 319 | 1.55e-49 | 168 |
| MS.gene016249.t1 | AT1G09500 | 36.491 | 285 | 172 | 5 | 1 | 281 | 1 | 280 | 4.47e-49 | 167 |
| MS.gene016249.t1 | AT2G33590 | 35.714 | 322 | 184 | 12 | 5 | 318 | 6 | 312 | 2.69e-44 | 154 |
| MS.gene016249.t1 | AT1G15950 | 34.268 | 321 | 198 | 9 | 8 | 326 | 13 | 322 | 9.00e-44 | 154 |
| MS.gene016249.t1 | AT1G15950 | 34.268 | 321 | 198 | 9 | 8 | 326 | 13 | 322 | 9.00e-44 | 154 |
| MS.gene016249.t1 | AT1G76470 | 33.333 | 315 | 203 | 5 | 5 | 317 | 4 | 313 | 3.59e-43 | 151 |
| MS.gene016249.t1 | AT1G80820 | 34.049 | 326 | 192 | 12 | 8 | 326 | 8 | 317 | 2.71e-42 | 149 |
| MS.gene016249.t1 | AT2G33600 | 36.646 | 322 | 181 | 11 | 5 | 318 | 6 | 312 | 3.99e-42 | 149 |
| MS.gene016249.t1 | AT1G15950 | 34.304 | 309 | 191 | 8 | 8 | 314 | 13 | 311 | 6.63e-42 | 148 |
| MS.gene016249.t1 | AT1G15950 | 35.897 | 273 | 163 | 8 | 8 | 278 | 13 | 275 | 1.42e-38 | 138 |
| MS.gene016249.t1 | AT1G15950 | 35.897 | 273 | 163 | 8 | 8 | 278 | 13 | 275 | 2.12e-38 | 138 |
| MS.gene016249.t1 | AT1G09490 | 33.559 | 295 | 187 | 6 | 35 | 326 | 3 | 291 | 2.22e-38 | 138 |
| MS.gene016249.t1 | AT1G15950 | 35.897 | 273 | 163 | 8 | 8 | 278 | 13 | 275 | 2.59e-38 | 138 |
| MS.gene016249.t1 | AT1G80820 | 35.612 | 278 | 157 | 11 | 8 | 278 | 8 | 270 | 2.73e-37 | 134 |
| MS.gene016249.t1 | AT1G09500 | 34.855 | 241 | 149 | 5 | 40 | 276 | 5 | 241 | 3.40e-33 | 124 |
| MS.gene016249.t1 | AT5G14700 | 30.479 | 292 | 168 | 10 | 4 | 276 | 51 | 326 | 3.18e-25 | 104 |
| MS.gene016249.t1 | AT1G68540 | 31.050 | 219 | 142 | 5 | 112 | 326 | 1 | 214 | 1.49e-24 | 99.4 |
| MS.gene016249.t1 | AT2G23910 | 28.339 | 307 | 193 | 10 | 9 | 303 | 11 | 302 | 2.04e-24 | 101 |
| MS.gene016249.t1 | AT2G23910 | 28.044 | 271 | 177 | 8 | 9 | 272 | 11 | 270 | 9.91e-22 | 93.2 |
| MS.gene016249.t1 | AT2G23910 | 28.044 | 271 | 177 | 8 | 9 | 272 | 11 | 270 | 1.00e-21 | 93.2 |
| MS.gene016249.t1 | AT5G14700 | 32.195 | 205 | 120 | 6 | 4 | 196 | 51 | 248 | 5.73e-21 | 91.3 |
| MS.gene016249.t1 | AT4G30470 | 27.244 | 312 | 187 | 12 | 9 | 302 | 11 | 300 | 3.06e-19 | 87.0 |
| MS.gene016249.t1 | AT1G15950 | 30.357 | 224 | 149 | 5 | 105 | 326 | 6 | 224 | 3.92e-17 | 80.1 |
| MS.gene016249.t1 | AT2G23910 | 26.966 | 267 | 169 | 9 | 49 | 303 | 16 | 268 | 1.37e-15 | 75.9 |
| MS.gene016249.t1 | AT4G33360 | 27.778 | 252 | 151 | 9 | 7 | 251 | 2 | 229 | 4.17e-15 | 75.5 |
| MS.gene016249.t1 | AT4G33360 | 27.778 | 252 | 151 | 9 | 7 | 251 | 14 | 241 | 4.34e-15 | 75.1 |
| MS.gene016249.t1 | AT4G33360 | 27.778 | 252 | 151 | 9 | 7 | 251 | 14 | 241 | 5.48e-15 | 75.1 |
Find 62 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTGATATTCTGGATATT+TGG | 0.178162 | 7.1:+28227031 | None:intergenic |
| GAGAAAGCTGTTCTTGAATT+TGG | 0.206019 | 7.1:-28227593 | MS.gene016249:CDS |
| ACTGTTAGTATTTGATATTC+TGG | 0.207664 | 7.1:+28227023 | None:intergenic |
| CTTGAATTTGGTGAACAAAA+TGG | 0.220583 | 7.1:-28227581 | MS.gene016249:CDS |
| ACAAAACGACCAACAATAAA+AGG | 0.257121 | 7.1:+28227533 | None:intergenic |
| ATCAAGAGTCTCCTTGAAAA+TGG | 0.290608 | 7.1:-28228715 | MS.gene016249:CDS |
| GATGAGTTGAAGGAAATTAA+AGG | 0.307837 | 7.1:-28226999 | MS.gene016249:CDS |
| TATTGAGAAGGCTCTTGTTT+TGG | 0.324795 | 7.1:-28227489 | MS.gene016249:CDS |
| GGTTCTGCTGTTTCATTCAA+TGG | 0.327787 | 7.1:-28227719 | MS.gene016249:CDS |
| ATTGCTGCAGCGAAACTGTC+TGG | 0.328131 | 7.1:+28227902 | None:intergenic |
| AGTTTCTTCGTGTTTAAATC+TGG | 0.331608 | 7.1:+28226966 | None:intergenic |
| AGAACCGTCGATGGCGCATT+AGG | 0.342006 | 7.1:-28227800 | MS.gene016249:CDS |
| CTTAGAAGTGTTAAGCCATT+TGG | 0.344973 | 7.1:-28227647 | MS.gene016249:CDS |
| TTATGCTGTTTCTAAGACTT+TGG | 0.354833 | 7.1:-28227618 | MS.gene016249:CDS |
| AAAGGAAGAGTATGTGTAAC+TGG | 0.362480 | 7.1:-28228766 | MS.gene016249:CDS |
| TCAATAGAATCAGGAAGCTT+AGG | 0.377093 | 7.1:+28227506 | None:intergenic |
| TTGAATTTGGTGAACAAAAT+GGG | 0.381597 | 7.1:-28227580 | MS.gene016249:CDS |
| CTGCTTGAGAATTCTGTTCC+AGG | 0.394830 | 7.1:-28227116 | MS.gene016249:CDS |
| TGACATTAACAGAACGTAAG+AGG | 0.420276 | 7.1:-28227997 | MS.gene016249:intron |
| TCACTCACGGCGAAGTCAAT+TGG | 0.434477 | 7.1:+28227845 | None:intergenic |
| AGTTGAGACATCTCTTCAAT+AGG | 0.440279 | 7.1:+28227062 | None:intergenic |
| CAATAGAATCAGGAAGCTTA+GGG | 0.444039 | 7.1:+28227507 | None:intergenic |
| AGAGCCTTCTCAATAGAATC+AGG | 0.454202 | 7.1:+28227497 | None:intergenic |
| ACTAACAGTAGATGAGTTGA+AGG | 0.472358 | 7.1:-28227009 | MS.gene016249:CDS |
| ATGTGTTGGATGAGAGTGAT+TGG | 0.477053 | 7.1:-28227685 | MS.gene016249:CDS |
| ACTGTCTGGGTTGCTTAGAT+CGG | 0.487171 | 7.1:+28227916 | None:intergenic |
| ATGCACCGGGTAGATTTGTC+AGG | 0.495956 | 7.1:+28227963 | None:intergenic |
| ACAGCATAATTCCAACCAAA+TGG | 0.504930 | 7.1:+28227632 | None:intergenic |
| GTCCAAATGGCTGAAGGAAA+AGG | 0.516695 | 7.1:-28228784 | None:intergenic |
| TCTTCAATAGGTACAATGAA+TGG | 0.521628 | 7.1:+28227074 | None:intergenic |
| CAGCAATCAAAGGATGTGTC+GGG | 0.524506 | 7.1:-28227886 | MS.gene016249:CDS |
| GAGCAATTGTATCTACCTCC+TGG | 0.524842 | 7.1:+28227098 | None:intergenic |
| ATTCAATGTTGCAAGGAGAA+AGG | 0.530160 | 7.1:-28226888 | MS.gene016249:CDS |
| AGTATGTGTAACTGGAGGTA+CGG | 0.533565 | 7.1:-28228758 | MS.gene016249:CDS |
| ACCTGGATCAGCTCTAATAG+TGG | 0.533677 | 7.1:+28228678 | None:intergenic |
| GCTTCCTGATTCTATTGAGA+AGG | 0.537211 | 7.1:-28227501 | MS.gene016249:CDS |
| TTGCTGCAGCGAAACTGTCT+GGG | 0.539457 | 7.1:+28227903 | None:intergenic |
| ACGAAGAAACTCGTGGATGC+TGG | 0.539479 | 7.1:-28226954 | MS.gene016249:CDS |
| TATTTCTTCTGGTTCACTCA+CGG | 0.542470 | 7.1:+28227832 | None:intergenic |
| GAAGTGTTAAGCCATTTGGT+TGG | 0.545559 | 7.1:-28227643 | MS.gene016249:CDS |
| TGGAAAAGACAATGATGTGT+TGG | 0.554067 | 7.1:-28227699 | MS.gene016249:CDS |
| TGAAGGAAATTAAAGGTGCA+AGG | 0.554380 | 7.1:-28226992 | MS.gene016249:CDS |
| ACCACTATTAGAGCTGATCC+AGG | 0.558931 | 7.1:-28228679 | MS.gene016249:intron |
| GCAGCAATCAAAGGATGTGT+CGG | 0.561014 | 7.1:-28227887 | MS.gene016249:CDS |
| AATGCCTAATGCGCCATCGA+CGG | 0.565238 | 7.1:+28227796 | None:intergenic |
| GACATTAACAGAACGTAAGA+GGG | 0.566904 | 7.1:-28227996 | MS.gene016249:intron |
| TTTCCACATGGTACATGTTG+AGG | 0.569682 | 7.1:-28227162 | MS.gene016249:CDS |
| GTATGTGTAACTGGAGGTAC+GGG | 0.572789 | 7.1:-28228757 | MS.gene016249:CDS |
| CATGGTACATGTTGAGGATG+TGG | 0.574986 | 7.1:-28227156 | MS.gene016249:CDS |
| AGTTTCGCTGCAGCAATCAA+AGG | 0.584297 | 7.1:-28227896 | MS.gene016249:CDS |
| AAGAGTGTCCAAATGGCTGA+AGG | 0.588094 | 7.1:-28228790 | None:intergenic |
| AGGTTTATATACACTTCAAG+TGG | 0.594598 | 7.1:-28227740 | MS.gene016249:CDS |
| GGCGAAGTCAATTGGTGAAG+CGG | 0.597111 | 7.1:+28227853 | None:intergenic |
| TGGTGTGACACGTTTCCACA+TGG | 0.600639 | 7.1:-28227174 | MS.gene016249:CDS |
| TGAATTCAAAGACAGTGAAG+AGG | 0.602314 | 7.1:-28227760 | MS.gene016249:CDS |
| CGATGCAATTCAATGTTGCA+AGG | 0.602642 | 7.1:-28226895 | MS.gene016249:CDS |
| AGTCAATTGGTGAAGCGGTG+TGG | 0.629454 | 7.1:+28227858 | None:intergenic |
| AGCTTCCTGACAAATCTACC+CGG | 0.636776 | 7.1:-28227968 | MS.gene016249:CDS |
| TTTAAACACGAAGAAACTCG+TGG | 0.659776 | 7.1:-28226961 | MS.gene016249:CDS |
| CTTGAGAATTCTGTTCCAGG+AGG | 0.688166 | 7.1:-28227113 | MS.gene016249:CDS |
| GGAAGAGTATGTGTAACTGG+AGG | 0.698516 | 7.1:-28228763 | MS.gene016249:CDS |
| CATCCTCAACATGTACCATG+TGG | 0.710484 | 7.1:+28227159 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTGAAAATATTAAAATAAAA+AGG | + | chr7.1:28227015-28227034 | None:intergenic | 10.0% |
| !! | AATTAACTATATTGTGTGTT+TGG | - | chr7.1:28227051-28227070 | MS.gene016249:CDS | 20.0% |
| !! | GTATATAGAAACAACTATAA+AGG | + | chr7.1:28227185-28227204 | None:intergenic | 20.0% |
| !! | TATTTGATATTCTGGATATT+TGG | + | chr7.1:28228628-28228647 | None:intergenic | 20.0% |
| !! | TGAATTTGACAAGATTATAA+TGG | + | chr7.1:28227319-28227338 | None:intergenic | 20.0% |
| !!! | ACATCAATTTATTTTCACTT+AGG | + | chr7.1:28228442-28228461 | None:intergenic | 20.0% |
| ! | AAATTCACAGTAATTCTGTT+AGG | - | chr7.1:28227332-28227351 | MS.gene016249:intron | 25.0% |
| ! | AATTGTGGTAAAAACTTCTT+TGG | - | chr7.1:28227272-28227291 | MS.gene016249:intron | 25.0% |
| ! | ACTGTTAGTATTTGATATTC+TGG | + | chr7.1:28228636-28228655 | None:intergenic | 25.0% |
| ! | GAAAAAACATGAGTAGAATA+AGG | + | chr7.1:28228213-28228232 | None:intergenic | 25.0% |
| ! | GGAAATTATAAGCATTTGTT+TGG | + | chr7.1:28227631-28227650 | None:intergenic | 25.0% |
| ! | TCTGTTAATGTCAAAAACAT+AGG | + | chr7.1:28227652-28227671 | None:intergenic | 25.0% |
| !! | AGAAGTTTTTACCACAATTA+CGG | + | chr7.1:28227271-28227290 | None:intergenic | 25.0% |
| !! | GTCAATTTAAAACCTAAACT+CGG | - | chr7.1:28228354-28228373 | MS.gene016249:intron | 25.0% |
| !! | TTGAATTTGGTGAACAAAAT+GGG | - | chr7.1:28228076-28228095 | MS.gene016249:intron | 25.0% |
| !! | TTGATTCTTCCTTTTATTGT+TGG | - | chr7.1:28228114-28228133 | MS.gene016249:intron | 25.0% |
| AAAAGGGTAATAAATTCACC+TGG | + | chr7.1:28226998-28227017 | None:intergenic | 30.0% | |
| ACAAAACGACCAACAATAAA+AGG | + | chr7.1:28228126-28228145 | None:intergenic | 30.0% | |
| AGGTTTATATACACTTCAAG+TGG | - | chr7.1:28227916-28227935 | MS.gene016249:CDS | 30.0% | |
| AGTTTCTTCGTGTTTAAATC+TGG | + | chr7.1:28228693-28228712 | None:intergenic | 30.0% | |
| CATATACAATTAGAGTCGTT+GGG | + | chr7.1:28228401-28228420 | None:intergenic | 30.0% | |
| GTGAAAATAAATTGATGTGC+AGG | - | chr7.1:28228444-28228463 | MS.gene016249:intron | 30.0% | |
| TCTTCAATAGGTACAATGAA+TGG | + | chr7.1:28228585-28228604 | None:intergenic | 30.0% | |
| TTCACAAATAGAGTAAAACC+TGG | + | chr7.1:28227132-28227151 | None:intergenic | 30.0% | |
| ! | ATGAGATCGTTGATAAAGAT+TGG | - | chr7.1:28228306-28228325 | MS.gene016249:intron | 30.0% |
| ! | CTTGAATTTGGTGAACAAAA+TGG | - | chr7.1:28228075-28228094 | MS.gene016249:intron | 30.0% |
| ! | GATGAGTTGAAGGAAATTAA+AGG | - | chr7.1:28228657-28228676 | MS.gene016249:intron | 30.0% |
| ! | GGTTTTACTCTATTTGTGAA+TGG | - | chr7.1:28227132-28227151 | MS.gene016249:CDS | 30.0% |
| ! | GTTGAAGAAATGTAGCTTTT+CGG | + | chr7.1:28227719-28227738 | None:intergenic | 30.0% |
| ! | TTCCAAGTCGATAAACTTTT+TGG | + | chr7.1:28227358-28227377 | None:intergenic | 30.0% |
| !! | AATGGTCATTAGTCTTGATT+TGG | - | chr7.1:28227150-28227169 | MS.gene016249:CDS | 30.0% |
| !! | ATGGTCATTAGTCTTGATTT+GGG | - | chr7.1:28227151-28227170 | MS.gene016249:CDS | 30.0% |
| !! | CTTTTTGTCACTATTTCTTC+TGG | + | chr7.1:28227838-28227857 | None:intergenic | 30.0% |
| !! | TATATTGTGTGTTTGGTTTG+TGG | - | chr7.1:28227058-28227077 | MS.gene016249:CDS | 30.0% |
| !! | TTATGCTGTTTCTAAGACTT+TGG | - | chr7.1:28228038-28228057 | MS.gene016249:intron | 30.0% |
| !!! | CATTTTTGTTTAACTTGACC+AGG | - | chr7.1:28227111-28227130 | MS.gene016249:CDS | 30.0% |
| AACGTTCATATTCCGAGTTT+AGG | + | chr7.1:28228369-28228388 | None:intergenic | 35.0% | |
| ACAGCATAATTCCAACCAAA+TGG | + | chr7.1:28228027-28228046 | None:intergenic | 35.0% | |
| AGTTGAGACATCTCTTCAAT+AGG | + | chr7.1:28228597-28228616 | None:intergenic | 35.0% | |
| ATCAAGAGTCTCCTTGAAAA+TGG | - | chr7.1:28226941-28226960 | MS.gene016249:CDS | 35.0% | |
| ATTCAATGTTGCAAGGAGAA+AGG | - | chr7.1:28228768-28228787 | MS.gene016249:CDS | 35.0% | |
| CAATAGAATCAGGAAGCTTA+GGG | + | chr7.1:28228152-28228171 | None:intergenic | 35.0% | |
| CTCGTATATATCTGAGTTCA+CGG | - | chr7.1:28227217-28227236 | MS.gene016249:intron | 35.0% | |
| GACATTAACAGAACGTAAGA+GGG | - | chr7.1:28227660-28227679 | MS.gene016249:CDS | 35.0% | |
| GAGAAAGCTGTTCTTGAATT+TGG | - | chr7.1:28228063-28228082 | MS.gene016249:intron | 35.0% | |
| GCAGGCAAAAAAGAACAAAT+TGG | - | chr7.1:28228462-28228481 | MS.gene016249:intron | 35.0% | |
| GCATATACAATTAGAGTCGT+TGG | + | chr7.1:28228402-28228421 | None:intergenic | 35.0% | |
| TAGAGAAACTGCAAAGGATT+TGG | - | chr7.1:28227478-28227497 | MS.gene016249:intron | 35.0% | |
| TCAATAGAATCAGGAAGCTT+AGG | + | chr7.1:28228153-28228172 | None:intergenic | 35.0% | |
| TGAATTCAAAGACAGTGAAG+AGG | - | chr7.1:28227896-28227915 | MS.gene016249:CDS | 35.0% | |
| TGACATTAACAGAACGTAAG+AGG | - | chr7.1:28227659-28227678 | MS.gene016249:CDS | 35.0% | |
| TGGAAAAGACAATGATGTGT+TGG | - | chr7.1:28227957-28227976 | MS.gene016249:CDS | 35.0% | |
| TTTAAACACGAAGAAACTCG+TGG | - | chr7.1:28228695-28228714 | MS.gene016249:CDS | 35.0% | |
| ! | AAAGGAAGAGTATGTGTAAC+TGG | - | chr7.1:28226890-28226909 | MS.gene016249:CDS | 35.0% |
| ! | ACTAACAGTAGATGAGTTGA+AGG | - | chr7.1:28228647-28228666 | MS.gene016249:intron | 35.0% |
| ! | TGAAGGAAATTAAAGGTGCA+AGG | - | chr7.1:28228664-28228683 | MS.gene016249:intron | 35.0% |
| !! | CTTAGAAGTGTTAAGCCATT+TGG | - | chr7.1:28228009-28228028 | MS.gene016249:intron | 35.0% |
| !! | GTGTTTGGTTTGTGGAATAT+GGG | - | chr7.1:28227066-28227085 | MS.gene016249:CDS | 35.0% |
| !! | TATTGAGAAGGCTCTTGTTT+TGG | - | chr7.1:28228167-28228186 | MS.gene016249:intron | 35.0% |
| !! | TATTTCTTCTGGTTCACTCA+CGG | + | chr7.1:28227827-28227846 | None:intergenic | 35.0% |
| !! | TGAATCCATCGACTGATTTT+AGG | - | chr7.1:28227454-28227473 | MS.gene016249:intron | 35.0% |
| !! | TGACAGAGTATCCATTTTCA+AGG | + | chr7.1:28226955-28226974 | None:intergenic | 35.0% |
| !! | TGTGTTTGGTTTGTGGAATA+TGG | - | chr7.1:28227065-28227084 | MS.gene016249:CDS | 35.0% |
| !!! | AAGGCTCTTGTTTTGGTATT+AGG | - | chr7.1:28228174-28228193 | MS.gene016249:intron | 35.0% |
| AGAGCCTTCTCAATAGAATC+AGG | + | chr7.1:28228162-28228181 | None:intergenic | 40.0% | |
| AGTATGTGTAACTGGAGGTA+CGG | - | chr7.1:28226898-28226917 | MS.gene016249:CDS | 40.0% | |
| ATGTGTTGGATGAGAGTGAT+TGG | - | chr7.1:28227971-28227990 | MS.gene016249:CDS | 40.0% | |
| CGATGCAATTCAATGTTGCA+AGG | - | chr7.1:28228761-28228780 | MS.gene016249:CDS | 40.0% | |
| GCTTCCTGATTCTATTGAGA+AGG | - | chr7.1:28228155-28228174 | MS.gene016249:intron | 40.0% | |
| TCTAGCCTAAAATCAGTCGA+TGG | + | chr7.1:28227462-28227481 | None:intergenic | 40.0% | |
| TTAGGCTAGAGAAACTGCAA+AGG | - | chr7.1:28227472-28227491 | MS.gene016249:intron | 40.0% | |
| TTTCCACATGGTACATGTTG+AGG | - | chr7.1:28228494-28228513 | MS.gene016249:intron | 40.0% | |
| ! | GAAGTGTTAAGCCATTTGGT+TGG | - | chr7.1:28228013-28228032 | MS.gene016249:intron | 40.0% |
| ! | GGCCAAAAAGTTTATCGACT+TGG | - | chr7.1:28227353-28227372 | MS.gene016249:intron | 40.0% |
| !! | CGACTTAGATTTAGCTTGAG+AGG | + | chr7.1:28227390-28227409 | None:intergenic | 40.0% |
| !! | GGTTCTGCTGTTTCATTCAA+TGG | - | chr7.1:28227937-28227956 | MS.gene016249:CDS | 40.0% |
| ACCACAATTACGGTGACATG+TGG | + | chr7.1:28227261-28227280 | None:intergenic | 45.0% | |
| ACCACATGTCACCGTAATTG+TGG | - | chr7.1:28227257-28227276 | MS.gene016249:intron | 45.0% | |
| ACCACTATTAGAGCTGATCC+AGG | - | chr7.1:28226977-28226996 | MS.gene016249:CDS | 45.0% | |
| ACTGTCTGGGTTGCTTAGAT+CGG | + | chr7.1:28227743-28227762 | None:intergenic | 45.0% | |
| AGCTTCCTGACAAATCTACC+CGG | - | chr7.1:28227688-28227707 | MS.gene016249:CDS | 45.0% | |
| AGTTTCGCTGCAGCAATCAA+AGG | - | chr7.1:28227760-28227779 | MS.gene016249:CDS | 45.0% | |
| CAGCAATCAAAGGATGTGTC+GGG | - | chr7.1:28227770-28227789 | MS.gene016249:CDS | 45.0% | |
| CATCCTCAACATGTACCATG+TGG | + | chr7.1:28228500-28228519 | None:intergenic | 45.0% | |
| CATGGTACATGTTGAGGATG+TGG | - | chr7.1:28228500-28228519 | MS.gene016249:intron | 45.0% | |
| CTGCTTGAGAATTCTGTTCC+AGG | - | chr7.1:28228540-28228559 | MS.gene016249:intron | 45.0% | |
| CTTGAGAATTCTGTTCCAGG+AGG | - | chr7.1:28228543-28228562 | MS.gene016249:intron | 45.0% | |
| GAGCAATTGTATCTACCTCC+TGG | + | chr7.1:28228561-28228580 | None:intergenic | 45.0% | |
| GCAGCAATCAAAGGATGTGT+CGG | - | chr7.1:28227769-28227788 | MS.gene016249:CDS | 45.0% | |
| GTGACAAAAAGAACCGTCGA+TGG | - | chr7.1:28227847-28227866 | MS.gene016249:CDS | 45.0% | |
| ! | ACCTGGATCAGCTCTAATAG+TGG | + | chr7.1:28226981-28227000 | None:intergenic | 45.0% |
| ! | ATGTAGCTTTTCGGATGCAC+CGG | + | chr7.1:28227710-28227729 | None:intergenic | 45.0% |
| ! | GGAAGAGTATGTGTAACTGG+AGG | - | chr7.1:28226893-28226912 | MS.gene016249:CDS | 45.0% |
| ! | GTACGGGTTTTCTTGGTTCA+TGG | - | chr7.1:28226915-28226934 | MS.gene016249:CDS | 45.0% |
| ! | GTATGTGTAACTGGAGGTAC+GGG | - | chr7.1:28226899-28226918 | MS.gene016249:CDS | 45.0% |
| !! | TGAAAATATTAAAATAAAAA+GGG | + | chr7.1:28227014-28227033 | None:intergenic | 5.0% |
| AATGCCTAATGCGCCATCGA+CGG | + | chr7.1:28227863-28227882 | None:intergenic | 50.0% | |
| ACGAAGAAACTCGTGGATGC+TGG | - | chr7.1:28228702-28228721 | MS.gene016249:CDS | 50.0% | |
| AGTCAATTGGTGAAGCGGTG+TGG | + | chr7.1:28227801-28227820 | None:intergenic | 50.0% | |
| ATGCACCGGGTAGATTTGTC+AGG | + | chr7.1:28227696-28227715 | None:intergenic | 50.0% | |
| ATTGCTGCAGCGAAACTGTC+TGG | + | chr7.1:28227757-28227776 | None:intergenic | 50.0% | |
| GGCGAAGTCAATTGGTGAAG+CGG | + | chr7.1:28227806-28227825 | None:intergenic | 50.0% | |
| TCACTCACGGCGAAGTCAAT+TGG | + | chr7.1:28227814-28227833 | None:intergenic | 50.0% | |
| TGGTGTGACACGTTTCCACA+TGG | - | chr7.1:28228482-28228501 | MS.gene016249:intron | 50.0% | |
| TTGCTGCAGCGAAACTGTCT+GGG | + | chr7.1:28227756-28227775 | None:intergenic | 50.0% | |
| ! | TGTAGCTTTTCGGATGCACC+GGG | + | chr7.1:28227709-28227728 | None:intergenic | 50.0% |
| !! | ACTGGAGGTACGGGTTTTCT+TGG | - | chr7.1:28226908-28226927 | MS.gene016249:CDS | 50.0% |
| AGAACCGTCGATGGCGCATT+AGG | - | chr7.1:28227856-28227875 | MS.gene016249:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 28226878 | 28228800 | 28226878 | ID=MS.gene016249 |
| chr7.1 | mRNA | 28226878 | 28228800 | 28226878 | ID=MS.gene016249.t1;Parent=MS.gene016249 |
| chr7.1 | exon | 28228680 | 28228800 | 28228680 | ID=MS.gene016249.t1.exon1;Parent=MS.gene016249.t1 |
| chr7.1 | CDS | 28228680 | 28228800 | 28228680 | ID=cds.MS.gene016249.t1;Parent=MS.gene016249.t1 |
| chr7.1 | exon | 28227483 | 28228007 | 28227483 | ID=MS.gene016249.t1.exon2;Parent=MS.gene016249.t1 |
| chr7.1 | CDS | 28227483 | 28228007 | 28227483 | ID=cds.MS.gene016249.t1;Parent=MS.gene016249.t1 |
| chr7.1 | exon | 28226878 | 28227212 | 28226878 | ID=MS.gene016249.t1.exon3;Parent=MS.gene016249.t1 |
| chr7.1 | CDS | 28226878 | 28227212 | 28226878 | ID=cds.MS.gene016249.t1;Parent=MS.gene016249.t1 |
| Gene Sequence |
| Protein sequence |
