AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene018109

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018109.t1 MTR_7g095080 96.966 725 21 1 1 724 1 725 0.0 1454
MS.gene018109.t1 MTR_4g094992 92.664 518 32 1 213 724 2 519 0.0 989
MS.gene018109.t1 MTR_1g023985 46.423 685 309 6 41 724 563 1190 0.0 633
MS.gene018109.t1 MTR_1g114490 45.727 667 304 6 59 724 512 1121 0.0 610
MS.gene018109.t1 MTR_3g023880 44.818 685 318 8 43 724 407 1034 0.0 591
MS.gene018109.t1 MTR_6g061840 44.234 685 321 7 41 720 247 875 0.0 581
MS.gene018109.t1 MTR_2g015320 40.851 705 340 7 41 723 46 695 0.0 552
MS.gene018109.t1 MTR_5g075310 41.850 681 296 6 43 720 204 787 0.0 541
MS.gene018109.t1 MTR_1g086640 41.086 718 346 10 27 724 33 693 0.0 540
MS.gene018109.t1 MTR_3g054400 40.462 692 346 8 39 722 359 992 2.72e-178 538
MS.gene018109.t1 MTR_2g098440 40.260 693 348 8 39 723 562 1196 1.02e-175 537
MS.gene018109.t1 MTR_3g052610 40.027 742 340 12 27 722 33 715 3.65e-175 518
MS.gene018109.t1 MTR_6g061840 46.235 571 246 7 41 606 247 761 4.72e-168 502
MS.gene018109.t1 MTR_4g485610 40.211 664 323 9 78 724 49 655 2.12e-166 495
MS.gene018109.t1 MTR_8g080240 38.171 689 343 14 47 712 3 631 5.82e-155 464
MS.gene018109.t1 MTR_8g080240 38.171 689 343 14 47 712 3 631 2.19e-154 464
MS.gene018109.t1 MTR_8g080240 38.390 646 319 13 47 673 3 588 3.47e-144 434
MS.gene018109.t1 MTR_8g041990 35.703 661 254 10 43 699 101 594 5.03e-124 384
MS.gene018109.t1 MTR_6g061840 49.606 381 151 1 41 421 247 586 2.19e-123 380
MS.gene018109.t1 MTR_3g086770 33.287 715 369 21 41 717 255 899 4.90e-97 330
MS.gene018109.t1 MTR_3g014030 32.394 710 382 18 41 717 255 899 1.90e-96 328
MS.gene018109.t1 MTR_0267s0070 32.943 683 362 18 66 717 937 1554 1.35e-91 314
MS.gene018109.t1 MTR_1g056490 30.663 724 416 17 45 712 228 921 2.23e-79 274
MS.gene018109.t1 MTR_1543s0010 41.026 312 162 6 327 633 10 304 4.60e-69 229
MS.gene018109.t1 MTR_4g023590 36.890 328 166 8 15 320 227 535 1.28e-54 204
MS.gene018109.t1 MTR_4g023590 38.624 189 114 2 332 519 692 879 3.73e-32 134
MS.gene018109.t1 MTR_3g055020 41.202 233 121 3 385 616 44 261 6.82e-53 189
MS.gene018109.t1 MTR_8g045110 28.406 433 288 7 288 712 105 523 6.67e-48 179
MS.gene018109.t1 MTR_1g109170 29.493 434 273 11 292 709 601 1017 7.33e-48 183
MS.gene018109.t1 MTR_1g109170 34.959 246 108 4 35 273 316 516 7.21e-32 134
MS.gene018109.t1 MTR_1g109170 29.630 432 271 11 294 709 603 1017 1.04e-47 182
MS.gene018109.t1 MTR_1g109170 34.959 246 108 4 35 273 316 516 7.75e-32 133
MS.gene018109.t1 MTR_8g106630 29.932 441 271 13 288 709 543 964 1.54e-46 179
MS.gene018109.t1 MTR_8g106630 35.294 238 110 3 35 269 260 456 2.15e-34 141
MS.gene018109.t1 MTR_1g088590 29.532 491 245 15 48 491 30 466 1.74e-43 169
MS.gene018109.t1 MTR_4g134790 33.243 370 216 9 280 630 888 1245 5.17e-42 165
MS.gene018109.t1 MTR_4g134790 33.096 281 133 6 7 273 562 801 3.27e-33 138
MS.gene018109.t1 MTR_4g134790 33.243 370 216 9 280 630 885 1242 5.96e-42 165
MS.gene018109.t1 MTR_4g134790 33.096 281 133 6 7 273 562 801 3.70e-33 137
MS.gene018109.t1 MTR_8g044260 28.341 434 281 10 291 709 521 939 5.21e-41 162
MS.gene018109.t1 MTR_8g044260 31.600 250 119 3 27 269 228 432 1.20e-30 129
MS.gene018109.t1 MTR_5g090430 28.241 432 273 12 273 682 526 942 6.04e-40 159
MS.gene018109.t1 MTR_5g090430 28.512 242 118 5 45 273 184 383 2.27e-25 113
MS.gene018109.t1 MTR_5g090430 28.241 432 273 12 273 682 413 829 6.83e-40 158
MS.gene018109.t1 MTR_5g090430 28.512 242 118 5 45 273 71 270 2.46e-25 112
MS.gene018109.t1 MTR_4g120070 32.734 278 129 7 77 320 208 461 1.26e-33 139
MS.gene018109.t1 MTR_4g120070 37.624 202 119 3 324 519 605 805 9.60e-33 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018109.t1 AT1G26370 70.203 688 203 1 35 720 26 713 0.0 1022
MS.gene018109.t1 AT3G26560 45.467 706 327 7 20 724 487 1135 0.0 633
MS.gene018109.t1 AT2G35340 44.336 715 336 9 13 724 371 1026 0.0 605
MS.gene018109.t1 AT2G35340 44.336 715 336 9 13 724 233 888 0.0 604
MS.gene018109.t1 AT1G32490 43.776 715 341 9 13 724 360 1016 0.0 602
MS.gene018109.t1 AT1G32490 43.776 715 341 9 13 724 370 1026 0.0 601
MS.gene018109.t1 AT4G16680 42.475 711 344 8 19 724 106 756 0.0 584
MS.gene018109.t1 AT3G62310 42.378 715 335 10 30 724 40 697 0.0 555
MS.gene018109.t1 AT2G47250 42.165 702 332 9 40 724 57 701 0.0 550
MS.gene018109.t1 AT4G18465 39.658 701 347 6 41 720 41 686 0.0 541
MS.gene018109.t1 AT5G13010 40.490 694 347 8 39 724 549 1184 1.66e-178 544
MS.gene018109.t1 AT3G62310 46.388 526 224 6 30 539 40 523 6.74e-152 453
MS.gene018109.t1 AT3G62310 46.168 535 227 7 30 545 40 532 1.64e-151 453
MS.gene018109.t1 AT4G16680 48.000 475 202 3 19 493 186 615 3.10e-150 452
MS.gene018109.t1 AT1G27900 36.797 693 358 14 47 715 3 639 5.79e-149 451
MS.gene018109.t1 AT1G27900 38.409 591 299 9 47 628 3 537 2.12e-140 423
MS.gene018109.t1 AT1G27900 38.409 591 299 9 47 628 3 537 5.01e-140 422
MS.gene018109.t1 AT5G10370 32.817 710 375 18 48 723 306 947 5.41e-96 327
MS.gene018109.t1 AT2G35920 30.748 722 418 18 45 712 167 860 9.69e-88 296
MS.gene018109.t1 AT4G01020 31.171 709 388 19 48 723 303 944 1.03e-87 302
MS.gene018109.t1 AT2G35920 30.748 722 418 18 45 712 225 918 2.17e-87 296
MS.gene018109.t1 AT2G35920 30.748 722 418 18 45 712 257 950 3.41e-87 296
MS.gene018109.t1 AT1G48650 29.625 746 449 19 16 709 267 988 6.04e-79 275
MS.gene018109.t1 AT1G48650 29.625 746 449 19 16 709 267 988 6.48e-79 275
MS.gene018109.t1 AT1G58050 29.726 693 382 24 14 636 561 1218 4.12e-69 247
MS.gene018109.t1 AT1G33390 38.276 290 137 5 40 312 233 497 3.92e-51 193
MS.gene018109.t1 AT1G33390 41.791 201 103 3 331 524 646 839 3.49e-38 153
MS.gene018109.t1 AT1G33390 38.276 290 137 5 40 312 233 497 3.92e-51 193
MS.gene018109.t1 AT1G33390 41.791 201 103 3 331 524 646 839 3.49e-38 153
MS.gene018109.t1 AT5G14900 34.615 286 160 8 448 724 3 270 3.36e-48 172
MS.gene018109.t1 AT2G01130 30.000 430 268 9 292 705 492 904 3.71e-48 184
MS.gene018109.t1 AT2G01130 34.553 246 117 3 27 269 199 403 6.42e-35 143
MS.gene018109.t1 AT2G01130 30.000 430 268 9 292 705 427 839 4.54e-48 184
MS.gene018109.t1 AT2G01130 34.553 246 117 3 27 269 134 338 6.26e-35 143
MS.gene018109.t1 AT1G58060 30.223 493 267 15 280 707 895 1375 2.86e-45 175
MS.gene018109.t1 AT1G58060 35.772 246 108 7 39 273 605 811 5.00e-35 144
MS.gene018109.t1 AT1G06670 28.788 396 252 9 247 630 524 901 1.60e-40 161
MS.gene018109.t1 AT1G06670 32.500 240 116 3 39 273 208 406 2.15e-31 132
MS.gene018109.t1 AT1G06670 28.788 396 252 9 247 630 524 901 1.88e-40 160
MS.gene018109.t1 AT1G06670 32.500 240 116 3 39 273 208 406 2.66e-31 132
MS.gene018109.t1 AT2G30800 27.673 477 292 15 274 716 184 641 2.02e-39 157
MS.gene018109.t1 AT2G30800 27.673 477 292 15 274 716 527 984 2.57e-39 157
MS.gene018109.t1 AT2G30800 30.417 240 121 3 39 273 178 376 1.48e-28 123
MS.gene018109.t1 AT5G04895 28.046 435 283 11 288 707 529 948 1.16e-38 155
MS.gene018109.t1 AT5G04895 30.315 254 125 3 27 273 239 447 1.65e-31 132
MS.gene018109.t1 AT2G47680 30.112 445 227 12 47 456 25 420 6.10e-38 152

Find 150 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGGTGTTGATGACATATT+AGG 0.230492 7.1:+12933737 MS.gene018109:CDS
GCATCTACATAATCTGTTTC+TGG 0.232424 7.1:-12930894 None:intergenic
GCCTTTGATCCGGCTCCATC+TGG 0.237514 7.1:+12931372 MS.gene018109:CDS
AGGTTGCAACGCTTAATTTC+TGG 0.256924 7.1:-12933686 None:intergenic
TTCTAAGAAAACTGTCATTC+TGG 0.285262 7.1:-12930722 None:intergenic
TTGATTATTGTTGGTGAAAC+TGG 0.291242 7.1:+12929572 MS.gene018109:CDS
ATAAGAGCTTTGGCATAAAC+TGG 0.292906 7.1:-12935124 None:intergenic
CACAGCTGCATTGAGGAAAA+AGG 0.328575 7.1:-12935894 None:intergenic
TTATGCACTCTGGTTTCTTC+CGG 0.338992 7.1:-12936246 None:intergenic
TTCATGTTGGATAAAGATAA+TGG 0.353602 7.1:+12930699 MS.gene018109:CDS
AAGTCACTTCCTATTGCTTC+GGG 0.354749 7.1:+12929422 MS.gene018109:CDS
TCTTTGCAATGCCTGGGACT+TGG 0.361472 7.1:-12932779 None:intergenic
TGTTTACAGGGAAGCATTGT+TGG 0.379684 7.1:+12930512 MS.gene018109:intron
CCCTTGAACGTGAACAGCTT+TGG 0.383415 7.1:-12930835 None:intergenic
TAACAGAGTACCCAACCTTT+TGG 0.385983 7.1:-12930125 None:intergenic
TTCTTTACACAACAGGGCTT+TGG 0.389466 7.1:+12936180 MS.gene018109:intron
CTCGTGCGGAAAATAAAATA+TGG 0.390077 7.1:-12935212 None:intergenic
GAGACATCAATTACAATTCC+TGG 0.391735 7.1:+12932667 MS.gene018109:CDS
CATACCTTCTTTGCAATGCC+TGG 0.393486 7.1:-12932786 None:intergenic
TGTCCACACTGATGTCTTGA+TGG 0.399891 7.1:+12930590 MS.gene018109:CDS
AAAGTCACTTCCTATTGCTT+CGG 0.400688 7.1:+12929421 MS.gene018109:CDS
GATGGAAGAGCTGCATATAT+AGG 0.404918 7.1:-12931333 None:intergenic
CAACCTATGGTTAACTGAAC+TGG 0.411358 7.1:+12936327 MS.gene018109:CDS
TGGGTGTTGATGACATATTA+GGG 0.411725 7.1:+12933738 MS.gene018109:CDS
TCAAGGACATGTTCAACAAA+TGG 0.413696 7.1:+12935811 MS.gene018109:CDS
TCAATTACATACTTGATTCC+AGG 0.417163 7.1:-12932685 None:intergenic
AGCAAGTCAGTTTAACTGCT+TGG 0.419602 7.1:+12935150 MS.gene018109:CDS
AGAGATTAATTGAAGAGGTT+CGG 0.420010 7.1:+12929537 MS.gene018109:CDS
GGCAAACAACTCATGGTGTT+TGG 0.423711 7.1:+12929135 None:intergenic
TAGAGCAGCTACTGATTCAT+TGG 0.426806 7.1:+12935579 MS.gene018109:CDS
GCCAAAGCTGTTCACGTTCA+AGG 0.430027 7.1:+12930834 MS.gene018109:CDS
GACAGCTATTATAAAATCAT+TGG 0.430046 7.1:+12935015 MS.gene018109:CDS
CGGGCCATTTGACGTCCAAC+TGG 0.430443 7.1:-12935088 None:intergenic
TTGGCGTTGGCATTAGCATT+GGG 0.434102 7.1:-12929204 None:intergenic
TAACCAGAGTTGACAACCTA+TGG 0.434887 7.1:+12936314 MS.gene018109:CDS
ACTTGCTAAAATAAGAGCTT+TGG 0.436656 7.1:-12935134 None:intergenic
ATCCTTCGTCTGTATTGTTC+CGG 0.437198 7.1:+12936227 MS.gene018109:CDS
TTTGGTAACAGTGGGCGTGC+AGG 0.437226 7.1:+12933590 MS.gene018109:intron
CCTTTGATCCGGCTCCATCT+GGG 0.438053 7.1:+12931373 MS.gene018109:CDS
CAACTCACAACCACATTCTT+CGG 0.438554 7.1:-12930100 None:intergenic
TGACAAGGTTCTCACCTTGA+AGG 0.442041 7.1:-12933779 None:intergenic
TGCCATGACGGAAAAGTTGT+TGG 0.442723 7.1:+12930027 MS.gene018109:CDS
ATTGGCGTTGGCATTAGCAT+TGG 0.442749 7.1:-12929205 None:intergenic
AAGGACCTGGAAAATGCTTT+CGG 0.443930 7.1:+12933618 MS.gene018109:CDS
TTGGTTATTGCGATTGGCGT+TGG 0.445640 7.1:-12929217 None:intergenic
AACTGGGTCCAAGGGAAGTC+GGG 0.447934 7.1:-12935107 None:intergenic
GACCTGGAAAATTGTTATCA+AGG 0.449776 7.1:-12930919 None:intergenic
TATTCTTGTTCTCATTATCA+AGG 0.450410 7.1:-12930668 None:intergenic
ATTTCAATACCCGAAGCAAT+AGG 0.452539 7.1:-12929431 None:intergenic
GACAATTATAACAGAATACT+TGG 0.453394 7.1:-12930547 None:intergenic
CAGGGCTTTGGCAAGTGGTC+AGG 0.455322 7.1:+12936192 MS.gene018109:intron
GACATGCTCGTGACATTCAC+AGG 0.455685 7.1:+12935692 MS.gene018109:CDS
CGTGCAGGGCGTGAAGGACC+TGG 0.456345 7.1:+12933605 MS.gene018109:CDS
GTGAGTTGGGCCAAAAGGTT+GGG 0.456369 7.1:+12930115 MS.gene018109:CDS
ATGGGTTTGGTTATTGCGAT+TGG 0.457755 7.1:-12929223 None:intergenic
GTAATACCAGGGAGAGGAAT+GGG 0.458451 7.1:-12929241 None:intergenic
ATCAAGTATGTAATTGATCC+TGG 0.458843 7.1:+12932691 MS.gene018109:CDS
AGTCCCAGGCATTGCAAAGA+AGG 0.461418 7.1:+12932782 MS.gene018109:CDS
CGTACTTTCTCTGAGTACTT+TGG 0.462297 7.1:+12930807 MS.gene018109:CDS
GCCATGACGGAAAAGTTGTT+GGG 0.462870 7.1:+12930028 MS.gene018109:CDS
ACATTCTTCGGCAACTCGCT+TGG 0.465098 7.1:-12930088 None:intergenic
GAAGAATGTGGTTGTGAGTT+GGG 0.470574 7.1:+12930102 MS.gene018109:CDS
TGATCCAGTTGGACGTCAAA+TGG 0.470663 7.1:+12935084 MS.gene018109:CDS
TAACCATAGGTTGTCAACTC+TGG 0.470971 7.1:-12936317 None:intergenic
TGCTGTTGAACGGCTTATCA+AGG 0.477511 7.1:+12931263 MS.gene018109:CDS
CGAAGAATGTGGTTGTGAGT+TGG 0.477627 7.1:+12930101 MS.gene018109:CDS
GCTGCAAAATGACATTAGAA+AGG 0.479362 7.1:-12933706 None:intergenic
GATATACTAGTATTTCTGAC+TGG 0.479761 7.1:+12931225 MS.gene018109:CDS
AGAGTGATGTGGTCTCCCTC+TGG 0.480341 7.1:-12935544 None:intergenic
TCAAATGGCCCGACTTCCCT+TGG 0.483015 7.1:+12935099 MS.gene018109:CDS
ACCAGGGAGAGGAATGGGTT+TGG 0.484017 7.1:-12929236 None:intergenic
GGCTTTGGCAAGTGGTCAGG+TGG 0.485781 7.1:+12936195 MS.gene018109:CDS
CAAGGACATGTTCAACAAAT+GGG 0.487040 7.1:+12935812 MS.gene018109:CDS
GCTCGCATTTGCTGCTCAGA+TGG 0.488003 7.1:-12931351 None:intergenic
CGGTCAAATTCTATCAAAGA+TGG 0.488070 7.1:+12930639 MS.gene018109:CDS
ATATTTGATCCTTGTTGAGT+TGG 0.489249 7.1:-12930166 None:intergenic
AAAGCGCGTTCCTATGATCC+TGG 0.491588 7.1:+12932721 MS.gene018109:CDS
TGAGAAGAGATTAATTGAAG+AGG 0.492285 7.1:+12929532 MS.gene018109:CDS
TTGGTAACAGTGGGCGTGCA+GGG 0.495052 7.1:+12933591 MS.gene018109:intron
CGTTCCTATGATCCTGGAAA+AGG 0.496489 7.1:+12932727 MS.gene018109:CDS
ATTTCATTGAGAAACCTTCA+AGG 0.501014 7.1:+12933765 MS.gene018109:CDS
TAATTTCGACGAGCCACAAA+AGG 0.507055 7.1:-12929177 None:intergenic
GCAAATGCGAGCCTTTGATC+CGG 0.507905 7.1:+12931362 MS.gene018109:CDS
ACAGAATACTTGGAAAGATA+AGG 0.508465 7.1:-12930537 None:intergenic
TGTGAGTTGGGCCAAAAGGT+TGG 0.511660 7.1:+12930114 MS.gene018109:CDS
CGTTGGCATTAGCATTGGGT+TGG 0.512423 7.1:-12929200 None:intergenic
GGATACCTGTAGGTTCCATC+TGG 0.513099 7.1:-12935923 None:intergenic
ATACCTTCTTTGCAATGCCT+GGG 0.513868 7.1:-12932785 None:intergenic
TCGTTAAATATTATGCACTC+TGG 0.514440 7.1:-12936256 None:intergenic
ACTTTCTCTGAGTACTTTGG+TGG 0.515214 7.1:+12930810 MS.gene018109:CDS
CCCAGATGGAGCCGGATCAA+AGG 0.518326 7.1:-12931373 None:intergenic
AGGATCAAATATATGACAGA+TGG 0.520985 7.1:+12930177 MS.gene018109:CDS
CACAACAGGGCTTTGGCAAG+TGG 0.525131 7.1:+12936187 MS.gene018109:intron
CAAATTCTATCAAAGATGGT+TGG 0.530800 7.1:+12930643 MS.gene018109:CDS
ATTGTTGGTGAAACTGGAAG+TGG 0.530851 7.1:+12929578 MS.gene018109:CDS
ACCAAACCCATTCCTCTCCC+TGG 0.534233 7.1:+12929235 MS.gene018109:CDS
GGTCTGAAACTTGCTTCTTG+TGG 0.535848 7.1:+12935833 MS.gene018109:CDS
CCAAAGCTGTTCACGTTCAA+GGG 0.541798 7.1:+12930835 MS.gene018109:CDS
GTGATAGCAGCAACACGTCT+AGG 0.547288 7.1:-12930060 None:intergenic
GAGCCAGTTCAGTTAACCAT+AGG 0.554841 7.1:-12936330 None:intergenic
TTGCGAAACCCAGATGGAGC+CGG 0.557730 7.1:-12931381 None:intergenic
CGAGTATAAAATATATCCAC+AGG 0.558690 7.1:-12930867 None:intergenic
AAACTGGGTCCAAGGGAAGT+CGG 0.561694 7.1:-12935108 None:intergenic
AAATGAATTTGAGAAACTTG+AGG 0.562155 7.1:+12933652 MS.gene018109:CDS
TGGTTGTGAGTTGGGCCAAA+AGG 0.566424 7.1:+12930110 MS.gene018109:CDS
AAGAGCTTCGCAAGTCCAGA+GGG 0.566562 7.1:+12935529 MS.gene018109:CDS
GAATACGAATGGTTTCATGT+TGG 0.567599 7.1:+12930686 MS.gene018109:CDS
GGCTCCATCTGGGTTTCGCA+AGG 0.567724 7.1:+12931383 MS.gene018109:CDS
GACATGATGATTAACTTCAA+CGG 0.568214 7.1:-12930768 None:intergenic
AGTGGGCGTGCAGGGCGTGA+AGG 0.569161 7.1:+12933599 MS.gene018109:intron
CTATGATCCTGGAAAAGGCA+TGG 0.570476 7.1:+12932732 MS.gene018109:CDS
ATATACTAGTATTTCTGACT+GGG 0.570954 7.1:+12931226 MS.gene018109:CDS
GGAACAATACAGACGAAGGA+TGG 0.575517 7.1:-12936225 None:intergenic
TGACAGATGGGTTACTTCTG+AGG 0.582783 7.1:+12930190 MS.gene018109:CDS
AGTAATACCAGGGAGAGGAA+TGG 0.583151 7.1:-12929242 None:intergenic
ACCTCTTCGCACTTCTCGTG+CGG 0.584338 7.1:-12935226 None:intergenic
GCAGCTGTGAAGCAGCCAGA+TGG 0.589847 7.1:+12935908 MS.gene018109:CDS
AAGAGATTGATGCTGTTGAA+CGG 0.590120 7.1:+12931253 MS.gene018109:CDS
GTCCACACTGATGTCTTGAT+GGG 0.592239 7.1:+12930591 MS.gene018109:CDS
GGAAAAGTAATACCAGGGAG+AGG 0.595830 7.1:-12929247 None:intergenic
AATGAGAACAAGAATACGAA+TGG 0.596334 7.1:+12930675 MS.gene018109:CDS
TTGGCATAAACTGGGTCCAA+GGG 0.596569 7.1:-12935115 None:intergenic
GGATCAAATATATGACAGAT+GGG 0.600655 7.1:+12930178 MS.gene018109:CDS
AAGCGAGTTGCCGAAGAATG+TGG 0.604736 7.1:+12930090 MS.gene018109:CDS
GCTGCTTCACAGCTGCATTG+AGG 0.605522 7.1:-12935901 None:intergenic
GATACCTTGCGAAACCCAGA+TGG 0.606659 7.1:-12931387 None:intergenic
AGCAGCCAGATGGAACCTAC+AGG 0.608253 7.1:+12935918 MS.gene018109:CDS
TTTGGCATAAACTGGGTCCA+AGG 0.609632 7.1:-12935116 None:intergenic
CACGAGCATGTCTAAGAGAA+CGG 0.611248 7.1:-12935681 None:intergenic
TTCCGCAGAAAGCATTGCAA+CGG 0.612393 7.1:-12935188 None:intergenic
AGAAATACTAGTATATCACC+AGG 0.613029 7.1:-12931219 None:intergenic
AGTACCTACATCCAAGTCCC+AGG 0.615133 7.1:+12932768 MS.gene018109:CDS
GTACATCAAGATGAGGCCCC+TGG 0.615632 7.1:+12931201 MS.gene018109:CDS
TAAGAGCTTCGCAAGTCCAG+AGG 0.617178 7.1:+12935528 MS.gene018109:CDS
AGCCGTTGCAATGCTTTCTG+CGG 0.618592 7.1:+12935186 MS.gene018109:CDS
ATTCTACTTCCAACTCAACA+AGG 0.634787 7.1:+12930157 MS.gene018109:CDS
TGTCAACTATCTGATCCAGT+TGG 0.635262 7.1:+12935073 MS.gene018109:CDS
AATGCCTGGGACTTGGATGT+AGG 0.635279 7.1:-12932772 None:intergenic
GTTATAGGTACATCAAGATG+AGG 0.635310 7.1:+12931194 MS.gene018109:intron
TAAGAGCTTTGGCATAAACT+GGG 0.636597 7.1:-12935123 None:intergenic
TCCGCACGAGAAGTGCGAAG+AGG 0.638157 7.1:+12935225 MS.gene018109:CDS
TGTCAACTCTGGTTAAATCA+CGG 0.646694 7.1:-12936306 None:intergenic
TTCCGGAACAATACAGACGA+AGG 0.647037 7.1:-12936229 None:intergenic
ACACATTAATCAGAGTGATG+TGG 0.651588 7.1:-12935555 None:intergenic
TCAAGGGAGACAGTTTCCTG+TGG 0.671336 7.1:+12930851 MS.gene018109:CDS
GAAATACTAGTATATCACCA+GGG 0.680918 7.1:-12931218 None:intergenic
TTCTGCATAGCGTAAAACTG+AGG 0.692959 7.1:-12936352 None:intergenic
AAATACTAGTATATCACCAG+GGG 0.695525 7.1:-12931217 None:intergenic
AGCCCATCAAGACATCAGTG+TGG 0.744619 7.1:-12930593 None:intergenic
ACGTGTTGCTGCTATCACTG+TGG 0.765431 7.1:+12930065 MS.gene018109:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.1 gene 12929147 12936378 12929147 ID=MS.gene018109
chr7.1 mRNA 12929147 12936378 12929147 ID=MS.gene018109.t1;Parent=MS.gene018109
chr7.1 exon 12929147 12929256 12929147 ID=MS.gene018109.t1.exon1;Parent=MS.gene018109.t1
chr7.1 CDS 12929147 12929256 12929147 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12929397 12929443 12929397 ID=MS.gene018109.t1.exon2;Parent=MS.gene018109.t1
chr7.1 CDS 12929397 12929443 12929397 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12929531 12929611 12929531 ID=MS.gene018109.t1.exon3;Parent=MS.gene018109.t1
chr7.1 CDS 12929531 12929611 12929531 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12929995 12930211 12929995 ID=MS.gene018109.t1.exon4;Parent=MS.gene018109.t1
chr7.1 CDS 12929995 12930211 12929995 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12930521 12930938 12930521 ID=MS.gene018109.t1.exon5;Parent=MS.gene018109.t1
chr7.1 CDS 12930521 12930938 12930521 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12931201 12931404 12931201 ID=MS.gene018109.t1.exon6;Parent=MS.gene018109.t1
chr7.1 CDS 12931201 12931404 12931201 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12932643 12932803 12932643 ID=MS.gene018109.t1.exon7;Parent=MS.gene018109.t1
chr7.1 CDS 12932643 12932803 12932643 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12933601 12933786 12933601 ID=MS.gene018109.t1.exon8;Parent=MS.gene018109.t1
chr7.1 CDS 12933601 12933786 12933601 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12935015 12935246 12935015 ID=MS.gene018109.t1.exon9;Parent=MS.gene018109.t1
chr7.1 CDS 12935015 12935246 12935015 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12935514 12935713 12935514 ID=MS.gene018109.t1.exon10;Parent=MS.gene018109.t1
chr7.1 CDS 12935514 12935713 12935514 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12935805 12935939 12935805 ID=MS.gene018109.t1.exon11;Parent=MS.gene018109.t1
chr7.1 CDS 12935805 12935939 12935805 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
chr7.1 exon 12936195 12936378 12936195 ID=MS.gene018109.t1.exon12;Parent=MS.gene018109.t1
chr7.1 CDS 12936195 12936378 12936195 ID=cds.MS.gene018109.t1;Parent=MS.gene018109.t1
Gene Sequence

>MS.gene018109

ATGGTGTTTGGTCAGAGAGATCGTAACAAGCCTTTTGTGGCTCGTCGAAATTACCAACCCAATGCTAATGCCAACGCCAATCGCAATAACCAAACCCATTCCTCTCCCTGGTATTACTTTTCCTAATTCATTTCAATTTTTACTCCTTTTCAACTATAATTATTCATGCTCAAAAGGGTAGCAGAAAAAGACCAAATTTTGAATTGGGTTTTAGTTTTTTTTTCTTTCTAATTTGTTAATTTTATTACAGGAGACAGAAAATTATAGAGCAGAGAAAGTCACTTCCTATTGCTTCGGGTATTGAAATTTGTTTGATTTTTGTGTTTTGAATGTTCTTACTTTCATATTTATCATGTGTGACCGTTGGCATTGTTTTTATTGTAGTTGAGAAGAGATTAATTGAAGAGGTTCGGAAAAATGATATTTTGATTATTGTTGGTGAAACTGGAAGTGGAAAAAGTACACGTAGGTTACATCTTATCCTATTAATCAATATGTTTATGATTATATCCTTTATAATTTAGGGTATATTTGGATTTACTTATCTGATTATTTACTCGCATAAGCACCTGTGAGAATGTTTGAGAGTTTTTATGAAAACAACAAATAACATGTTCATAAGCTGTTAGATTTGAAAAGAGCTTATAGCTTATATGAAAACAATTTGGCTTTGTTTTATCTTTTGTTTAAAAATAATTTATGTTGTATGATAAGCGCTTATGTTGTACTTGCATAATTTAGCTGTTTATTAAAACATGCCTTAGGTTTTTAGGAAAAGAAACGAAAAAAGGAATTTTAGAGATAGCGGGTTTTCACTGTGTTTATGTTTATTGCATTTTACCGTGTAGAGATTCCACAATTTTTGTTTCATACTGGATTTTGCCATGACGGAAAAGTTGTTGGGATAACTCAACCTAGACGTGTTGCTGCTATCACTGTGGCCAAGCGAGTTGCCGAAGAATGTGGTTGTGAGTTGGGCCAAAAGGTTGGGTACTCTGTTAGATTTGATGATTCTACTTCCAACTCAACAAGGATCAAATATATGACAGATGGGTTACTTCTGAGGTACAACTTTTTTGTAGCTTTGATTTCTGGGAGTGTTTCCTATCAAGACATGATACTGTGATTTGTTTTTTCTTTTCATTCTTGATGTCAAACTATAAAAATACCATATCACTTTTTTTGAATCAATTGTATTCAACGACGGGTGAGTAACATCCCCTTCCCCGTCGTTGATGCAGGTTTGAGATAGTTATATGATTTGAAGTAGTTAAGGAGGTTTCAGATTAAATATTTTGTTTTTCATGGTTTGTATTTGTAAGTACTGTTTTTCTTATGGTAAAGTTAATAAGCATTTTTTACTTGTGTTTACAGGGAAGCATTGTTGGATCCTTATCTTTCCAAGTATTCTGTTATAATTGTCGATGAAGCACACGAGAGAACTGTCCACACTGATGTCTTGATGGGCTTGCTAAAAAATGTGCAACATGCTCGGTCAAATTCTATCAAAGATGGTTGGAACCTTGATAATGAGAACAAGAATACGAATGGTTTCATGTTGGATAAAGATAATGGCCAGAATGACAGTTTTCTTAGAAAAGACCACCGCGAAAAGAGTTCTCCGTTGAAGTTAATCATCATGTCTGCAAGTCTTGATGCTCGTACTTTCTCTGAGTACTTTGGTGGTGCCAAAGCTGTTCACGTTCAAGGGAGACAGTTTCCTGTGGATATATTTTATACTCGTCATCCAGAAACAGATTATGTAGATGCTGCCTTGATAACAATTTTCCAGGTCATTGGTTTAATCAGTACATGTAATAATTTAATAACGTGGTTTAACTTATTTTTTTGTAAGAGACCTTAGCTCCTAATAATATTTTTTTGGGACAGTGGTTTTTTGGTATGCATTTATGGTACATGTTTGTGCACTGCCTCAGTCATTCAGACATACTGCTACACACACACACACACACAAGATTATAACATTGTGGTGATGGCTGAGAAGTTAAGTTCATATAATTGTCACTTAAAGTCATGTGTCTTTGATGTTATAGGTACATCAAGATGAGGCCCCTGGTGATATACTAGTATTTCTGACTGGGCAAGAAGAGATTGATGCTGTTGAACGGCTTATCAAGGAGCGACTTACAAAGTTACCTCAAGAAAATCAGAAGCTTCAAGTTGTGCCTATATATGCAGCTCTTCCATCTGAGCAGCAAATGCGAGCCTTTGATCCGGCTCCATCTGGGTTTCGCAAGGTATCCTGTAGCTATGCATATTATCCTTTGTTAAATTATCGTATTACTTCTGTGTAGAATTCAATAGAAAAATCAGGAAAATAAGTTTAATTTAACCAATCATTATTTTTTTATTTAATTTAAACGGTTGACATTTCCATCTAGGTTTTTATTTATTCTCTTGAAGCACTCTGTTTCCCATGACCATGTTAATTTATAAAGTAAATGCTGGTTCCTGATTGCCGCATTTAGTGCCTCAGCGTGTTTAGAACATATGCTCGTGCTTTGATATTTCATATTTTGTTATATGGAAGTTTCATCATTGGAAGTTCTCTTAGACACATATGACTTATATAATGATCATCTTCTGTTAGAAGTTAGAACTGGTATCGAAAGTAGAAAAAGTAAGATCGAAAAATGAAAATTGCAAAGGAGTGGTTCTTGAATTGATAACAATATGCACCCTGGCCAGTATATCATAGTGGTGGTGGATACACCAGCCTCGGGTTTCAAGTACCTGCAACTTCCAAATTCTACAATCCCCACAAACTCACCACTTTCTAGTTCCACCTGTATTTATAAGTTATAACTCCCTCTTCCTGTCTCTCCTTGATTAGTTATTCAGATGAAATGTATTTCCTATACTGTCCCCTGCTCTTCCTCATATCACTATAAATTACTAGAAGTTTCCTAACATCTTCCATTTACATTCATGATTTGTTACCTATAGTCTCTATCATTTGACAGTGTTGCCATTGTGGTCCAATTACTCTGATGTCAAATTTAGAACTAATTTTTTTTTTCTTCCAATGTCATTATTTGCTTTGTTGCTTCTAACAGTGGATCACTTTGGTTGTAAAGTACTATTTGTATCGTTTTTATACCGTCATACTTACCGAAATGCACCTACCAGTATCTATGTATACCGACCTATTATTTTCCTTTACTTATAACCTTCATCGTGTAATATGTTACCATCACCTATACTCCTTTCGGAACTATGCCTTCTGAACTGGCAACAATCCCTATAAATAGTACATGGATATACAAAACAGTGTCTGCTAGGAGTAGGACCATATTTTTTTCCCATAGTAGAGTGTCACATTTTTAGTTTGAGCCTGTGAATAACAATAACACCAATCAAGCCTTTAATGTGAGCTAATTTATATTCAAAGTAACCTCTGATATTTAATATTTATCATAGCAGGTAATCGCAATTCTTAAATTGTATTCAACTTGGTACTTTGTTGTTTTGTTAGGTGGTATTGGCAACTAATATTGCTGAGACATCAATTACAATTCCTGGAATCAAGTATGTAATTGATCCTGGACTTGTAAAAGCGCGTTCCTATGATCCTGGAAAAGGCATGGAATCTTTGATTGTAGTACCTACATCCAAGTCCCAGGCATTGCAAAGAAGGTATGGTGCTGGATTATGTTTCAATCTGTTTAATATTTGATTCTGTTTGATGTTTAATTGCTGCTGCAATTGTTGGTTGAATTTAGTTAATGTAATGTAAATGAAGTAAGTTTGGTTCAGTTTAACTGAGGATGACTTCCTTTCTCATTTCAAGTTCAGTTTATTAATGGATACCTTTGATTTTCCTCAAAAACATGTATTTTCTTAACAAAGGTTTTTGAAATGTGAATCTAGCATCATTATTTCTAGCACTTGTTTTTTGTTAAACATGGCGAGGCATAACAACAATTAAGCAAAACCTTATCCCACTGGTTGTGGTCGGCTATGAGTCAAATGGTGTCATAATTCAAATGACATCATAATCAAAACCATTGATTTCCCTCAAAAACATGTATTTTCTTAACAAAGACTTTTGGAACGTGAATCTAGCATCATTGTTTCTAGAACTTGTTTTTTATAACACATACCGAGGCATTACAACAACAATAATAAAACCTTAATCCACTAGTTGGGGTCGGCTATATGAGTCAAATGACGTCGTAGTTCCCTATCATGTGATAAGTTCAGAAATAGATTCTCTACTTCTAAATCTCTCTTGATGGTTTGGCCTATAGTTTTTCATGGTCTCACTCCACCTTTAACCATTGGACTGTCCTCTATCTAGCAAACTTTCCTTGACAGTGTCGTGTGTGTGCACTCGTCCATCTTAACCTTCTCATTATGTGACATAGGTAATATATTTAATTTGATTTTGCTATAAGAGTAGTGCAAATTGATTATCTATATTTTGGTAACAGTGGGCGTGCAGGGCGTGAAGGACCTGGAAAATGCTTTCGGCTATATCCAGAAAATGAATTTGAGAAACTTGAGGATTCTACAATGCCAGAAATTAAGCGTTGCAACCTTTCTAATGTCATTTTGCAGCTTAAGGCTTTGGGTGTTGATGACATATTAGGGTTCGATTTCATTGAGAAACCTTCAAGGTGAGAACCTTGTCAAAATTTTGGTTTGACAACTTCACTACCTATGCATATTTGTGGATAAGTTCTGATTGCTTATGTTTTTAAGGCTAAATTACTATGTAGATTTTAAAGTTTCACTTTGTTTTTAAATAGGTTCTTACACTTCAAAAAAATTAGTGTCTGTGATTCTAAAGATTTTTGTACGAGGGTCAACTACCAAGTACTATTAAGACAATGCAAGCTTTTGCTTAATTTGGAGACCCATTTGAAAGCATAGATAAGCTGAAGAGTTTGAGAGATATCACAGTTAAAAAGGCCCCTATACACTTAATCTTAATTTTCAAATAGGTCCTTATAAGCTATAATTTTGCTAAAGTTAACAGCCATTAGGGAGTAGGGATCTTATAAATTTGTCTAAACTATAAGAACCTATTTGAAAATTCATGAAACTATAGGGATCTGCAGACTAATGAAGACTATTTAAAATAAAATATGCATGTCCTGAAAGCTTTTTTTTCTAACTTCTGCTTATCTTTTAAAAGGATTTTAAGTCACTTGAAAAGATGCTATTATCTCTCTACTAAGATGATTCCTTCAACCTATATAGGTCCTTTTGATTAACCTTTGATTTCTTTTTGTTGTAATGTGATCTAAACTGGAGATAAAAAATCCATTTGAATATTAGGTGCTTTAATTCAATTTTTCTTGGTCATGAAAAATAAAATTATTGAGGATTGACTATATTGTATTCATATACCGAAAGAATAAGAATGACATGACATTTGTATTGACAAATAACCAAAAATCTATAAAAGTGTCATATATATTATTAATGCAAAAGTACTTTTAGGAATACTTGTATACTGATGAAGAAACTGAGAAGAGTACATAGACAACACCTAATAAAACCTTGCTTGGACCAAGATCTTGCACCTTAAAATCAATCTATAAAGATAAATTTCTATTACAAAATATTGAAAGTGGGCTTTAGTGAATTTTTTTGGACTGAATGGAGAAAATAAATAAAGGATGTAGATAATCTATTCCTTTGCATCAGTTATTAATAGTGAGTTCAAATATTTGAAACTTCTATTGATTCAGTTTCTTTAATGTCAGTTGTCTCGTCCATTGTACTCCAGAACTAAATTAACATTTTTTTAGAGTTATTCGTTTCTTTCTTTATTTTCGAAAAGTAATATCACTGAGCAACATATTACTCAAAGCTTGTACCTGTCACATGTTTTAACAGGACAGCTATTATAAAATCATTGGAGCAGCTATTTTTGTTAGGTGCTTTGACAGATGACTGTCAACTATCTGATCCAGTTGGACGTCAAATGGCCCGACTTCCCTTGGACCCAGTTTATGCCAAAGCTCTTATTTTAGCAAGTCAGTTTAACTGCTTGGAAGAGATGTTGATAGCCGTTGCAATGCTTTCTGCGGAATCCATATTTTATTTTCCGCACGAGAAGTGCGAAGAGGTTTGAAGAGTGAATTCGGATTGTAAACCATTCCTGTCATATTCTATTATTCTATTTCGGTGAAATGTAAATACGAGCATAAATCATTGATTATGTGCTATGCCACTCTATTGGTATATTCACAGTTTAACAAAATATTTGAAAAATATCATACGGATGGTTTAACTATATGCGATTGGTAATTACCGTAGGAAGATTCTAGTCTTTATTTGACGTCAACTTGTCTTCTCTACACTAACTTATTGTGCTTCTCTGATTTATGGCCAGGCAAGAACTGCAGCTAAGAGCTTCGCAAGTCCAGAGGGAGACCACATCACTCTGATTAATGTGTATAGAGCAGCTACTGATTCATTGGAGAAGAGAACTATCGAAACTAATAAAACAAAAAATGAAAAGGATTTTAGAAAATGGTGCAAAGAAAATTTTATAAATAGCCGTTCTCTTAGACATGCTCGTGACATTCACAGGTTGTTTTATTGTGTTTAAACTTTACTTCAAGGCATCTATAATATCCTTGAGACATTTTATGCATTCTAATTTCTGAAATGCCTTTTTCAGGCAGATTCAAGGACATGTTCAACAAATGGGTCTGAAACTTGCTTCTTGTGGAGAAGATATGCTTCAATTTCGCAGATGCCTTGTTGCTTCCTTTTTCCTCAATGCAGCTGTGAAGCAGCCAGATGGAACCTACAGGTATCCATTTTAACTTCAAAATTCTTGGGCAGTCCCGCTAGGGTCGCTTGCCATCTAATTGGAATCATTACATGAAAATATCGTTTAGTTTCGGGATTTTTCATTGGTAATTATGATTCGCTAATTGGAATCCTGACCTTACATGCATATACACTCTTCAAATATTTTGCTTTACACTCTTCAAATATTTTGCTTTTTTGTTTTTACTTATACTTTTATGATAAAAAAGACTCTTACTGCTTCTTTACACAACAGGGCTTTGGCAAGTGGTCAGGTGGTGCAGATCCATCCTTCGTCTGTATTGTTCCGGAAGAAACCAGAGTGCATAATATTTAACGAACTGATTCAAACTAATAACAAGTATGTCCGTGATTTAACCAGAGTTGACAACCTATGGTTAACTGAACTGGCTCCTCAGTTTTACGCTATGCAGAATTGA
Protein sequence

>MS.gene018109.t1

MVFGQRDRNKPFVARRNYQPNANANANRNNQTHSSPWRQKIIEQRKSLPIASVEKRLIEEVRKNDILIIVGETGSGKSTQIPQFLFHTGFCHDGKVVGITQPRRVAAITVAKRVAEECGCELGQKVGYSVRFDDSTSNSTRIKYMTDGLLLREALLDPYLSKYSVIIVDEAHERTVHTDVLMGLLKNVQHARSNSIKDGWNLDNENKNTNGFMLDKDNGQNDSFLRKDHREKSSPLKLIIMSASLDARTFSEYFGGAKAVHVQGRQFPVDIFYTRHPETDYVDAALITIFQVHQDEAPGDILVFLTGQEEIDAVERLIKERLTKLPQENQKLQVVPIYAALPSEQQMRAFDPAPSGFRKVVLATNIAETSITIPGIKYVIDPGLVKARSYDPGKGMESLIVVPTSKSQALQRSGRAGREGPGKCFRLYPENEFEKLEDSTMPEIKRCNLSNVILQLKALGVDDILGFDFIEKPSRTAIIKSLEQLFLLGALTDDCQLSDPVGRQMARLPLDPVYAKALILASQFNCLEEMLIAVAMLSAESIFYFPHEKCEEARTAAKSFASPEGDHITLINVYRAATDSLEKRTIETNKTKNEKDFRKWCKENFINSRSLRHARDIHRQIQGHVQQMGLKLASCGEDMLQFRRCLVASFFLNAAVKQPDGTYRALASGQVVQIHPSSVLFRKKPECIIFNELIQTNNKYVRDLTRVDNLWLTELAPQFYAMQN