AlfalfaGEDB | MS.gene018557

M. sativa cultivar XinJiangDaYe / MS.gene018557

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018557.t1	XP_020212665.1	98.6	138	2	0	1	138	1	138	3.10E-41	177.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018557.t1	Q1SU99	99.3	138	1	0	1	138	1	138	2.2e-43	176.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018557.t1	A0A151TUN5	98.6	138	2	0	1	138	1	138	2.3e-41	177.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene018557	MS.gene066301	PPI
MS.gene018557	MS.gene049822	PPI
MS.gene004127	MS.gene018557	PPI
MS.gene004126	MS.gene018557	PPI
MS.gene025425	MS.gene018557	PPI
MS.gene018557	MS.gene65249	PPI
MS.gene018557	MS.gene08465	PPI
MS.gene018557	MS.gene99525	PPI
MS.gene65007	MS.gene018557	PPI
MS.gene018557	MS.gene025426	PPI
MS.gene018557	MS.gene64653	PPI
MS.gene018557	MS.gene066300	PPI
MS.gene018557	MS.gene049824	PPI
MS.gene018557	MS.gene041623	PPI
MS.gene018557	MS.gene025035	PPI
MS.gene018557	MS.gene24295	PPI
MS.gene018557	MS.gene26950	PPI
MS.gene018557	MS.gene63143	PPI
MS.gene018557	MS.gene051008	PPI
MS.gene018557	MS.gene81051	PPI
MS.gene066348	MS.gene018557	PPI
MS.gene035396	MS.gene018557	PPI
MS.gene018557	MS.gene63953	PPI
MS.gene018557	MS.gene84498	PPI
MS.gene018557	MS.gene27326	PPI
MS.gene75219	MS.gene018557	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018557.t1	MTR_7g099960	99.275	138	1	0	1	138	1	138	1.85e-94	268
MS.gene018557.t1	MTR_4g063240	85.811	148	10	3	1	138	1	147	2.72e-81	236
MS.gene018557.t1	MTR_1g090780	93.525	139	6	3	1	138	1	137	6.25e-79	229
MS.gene018557.t1	MTR_1g492910	93.525	139	6	3	1	138	1	137	6.82e-79	229
MS.gene018557.t1	MTR_4g063220	86.577	149	8	3	1	138	1	148	1.51e-77	226
MS.gene018557.t1	MTR_4g063990	86.577	149	8	3	1	138	1	148	1.51e-77	226
MS.gene018557.t1	MTR_4g063200	86.577	149	8	3	1	138	1	148	1.51e-77	226
MS.gene018557.t1	MTR_4g064020	86.577	149	8	3	1	138	1	148	1.51e-77	226
MS.gene018557.t1	MTR_4g071180	86.577	149	8	3	1	138	1	148	1.41e-76	224
MS.gene018557.t1	MTR_4g070240	86.577	149	8	3	1	138	1	148	1.41e-76	224
MS.gene018557.t1	MTR_4g088025	87.500	144	11	3	1	138	1	143	4.51e-75	220
MS.gene018557.t1	MTR_4g070245	85.235	149	10	3	1	138	1	148	5.68e-75	220
MS.gene018557.t1	MTR_4g063275	83.893	149	12	3	1	138	1	148	2.52e-74	218
MS.gene018557.t1	MTR_4g064983	85.517	145	9	3	1	134	1	144	6.02e-73	218
MS.gene018557.t1	MTR_1g068600	95.370	108	4	1	31	138	30	136	5.60e-65	194
MS.gene018557.t1	MTR_2g084480	60.674	89	35	0	49	137	136	224	2.78e-37	127
MS.gene018557.t1	MTR_8g069990	71.910	89	22	2	9	97	3	88	1.07e-35	119
MS.gene018557.t1	MTR_4g063270	90.196	51	5	0	76	126	1	51	4.41e-27	95.9
MS.gene018557.t1	MTR_7g114580	90.244	41	4	0	98	138	11	51	5.92e-20	77.8
MS.gene018557.t1	MTR_4g032777	39.744	78	47	0	45	122	33	110	7.57e-13	61.6
MS.gene018557.t1	MTR_2g083800	66.071	56	14	3	9	62	11	63	3.72e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018557.t1	AT2G37470	89.855	138	13	1	1	137	1	138	8.42e-79	229
MS.gene018557.t1	AT3G53650	89.855	138	14	0	1	138	1	138	1.22e-77	226
MS.gene018557.t1	AT3G45980	82.781	151	12	3	1	138	1	150	9.13e-75	219
MS.gene018557.t1	AT1G07790	82.550	149	14	2	1	138	1	148	8.27e-74	217
MS.gene018557.t1	AT2G28720	82.237	152	12	3	1	138	1	151	1.86e-73	216
MS.gene018557.t1	AT5G59910	85.926	135	17	1	6	138	16	150	1.53e-71	211
MS.gene018557.t1	AT3G46030	88.372	129	13	1	12	138	17	145	5.88e-71	209
MS.gene018557.t1	AT5G02570	88.550	131	9	2	11	138	5	132	2.03e-67	200
MS.gene018557.t1	AT5G22880	81.119	143	18	2	5	138	3	145	5.66e-66	197
MS.gene018557.t1	AT3G09480	82.114	123	21	1	16	138	5	126	8.28e-64	191
MS.gene018557.t1	AT1G08170	60.000	85	34	0	51	135	151	235	3.06e-37	127

Find 34 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAAGAGCTGGTGAATT+TGG	0.235894	7.4:-10297910	None:intergenic
CTCCGCCTTTGGTTTCTTCT+CGG	0.278156	7.4:-10297574	None:intergenic
GCTGTTAGATTGGTTCTTCC+TGG	0.314946	7.4:+10297843	MS.gene018557:CDS
GAGATCTTCTTCTCCGCCTT+TGG	0.325858	7.4:-10297585	None:intergenic
ATTGGTTCTTCCTGGAGAAT+TGG	0.353160	7.4:+10297851	MS.gene018557:CDS
GCCTTTGGTTTCTTCTCGGC+TGG	0.357848	7.4:-10297570	None:intergenic
CATCTTCAAGGTTCTGAAAC+AGG	0.359264	7.4:+10297674	MS.gene018557:CDS
AAACAGGTTCATCCTGATAT+TGG	0.374570	7.4:+10297690	MS.gene018557:CDS
GATTCAAACTGCTGTTAGAT+TGG	0.401031	7.4:+10297833	MS.gene018557:CDS
TGCTCAGGAATCGTCTCGTT+TGG	0.412474	7.4:+10297773	MS.gene018557:CDS
TTGCTTGAAACACCAATATC+AGG	0.417581	7.4:-10297702	None:intergenic
TGGTGTTTCAAGCAAGGCTA+TGG	0.418611	7.4:+10297710	MS.gene018557:CDS
TCGGCTGGAGCTTTCTCTGC+GGG	0.433183	7.4:-10297555	None:intergenic
TGCTGTTTCTGAAGGGACTA+AGG	0.469671	7.4:+10297881	MS.gene018557:CDS
ATACAAGATATACATCTTCA+AGG	0.488494	7.4:+10297662	MS.gene018557:CDS
GCTAAACATGCTGTTTCTGA+AGG	0.504101	7.4:+10297873	MS.gene018557:CDS
TCTGCGGGCTTCTTCTCCGC+GGG	0.504996	7.4:-10297540	None:intergenic
GCATGTTTAGCCAATTCTCC+AGG	0.514866	7.4:-10297861	None:intergenic
TATCTTTGAGAAACTTGCTC+AGG	0.528928	7.4:+10297758	MS.gene018557:CDS
ATCTCCCTTGAAGTGATCGT+CGG	0.552358	7.4:-10297813	None:intergenic
CTCTGCGGGCTTCTTCTCCG+CGG	0.552987	7.4:-10297541	None:intergenic
GGTGTTTCAAGCAAGGCTAT+GGG	0.553844	7.4:+10297711	MS.gene018557:CDS
CTCGGCTGGAGCTTTCTCTG+CGG	0.557332	7.4:-10297556	None:intergenic
AGAAGCCGACGATCACTTCA+AGG	0.560392	7.4:+10297808	MS.gene018557:CDS
GAGAAGAAGATCTCAAAAGA+AGG	0.561490	7.4:+10297594	MS.gene018557:CDS
TGATATTGGTGTTTCAAGCA+AGG	0.568075	7.4:+10297704	MS.gene018557:CDS
AGGAATCGTCTCGTTTGGCT+AGG	0.569875	7.4:+10297778	MS.gene018557:CDS
AAAGCAACAAAATCAAGAGC+TGG	0.570887	7.4:-10297919	None:intergenic
CTAAACATGCTGTTTCTGAA+GGG	0.585084	7.4:+10297874	MS.gene018557:CDS
ATCAAGAATGGCTCCCACAA+AGG	0.630896	7.4:+10297506	None:intergenic
TCCAGCCGAGAAGAAACCAA+AGG	0.637685	7.4:+10297569	MS.gene018557:CDS
AAAGGCAGAAAAGAAACCCG+CGG	0.639354	7.4:+10297524	MS.gene018557:CDS
GAAGCCGACGATCACTTCAA+GGG	0.644853	7.4:+10297809	MS.gene018557:CDS
AGCCGAGAAGAAACCAAAGG+CGG	0.671315	7.4:+10297572	MS.gene018557:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACAAGATATACATCTTCA+AGG	+	chr7.4:10297662-10297681	MS.gene018557:CDS	25.0%
	AAACAGGTTCATCCTGATAT+TGG	+	chr7.4:10297690-10297709	MS.gene018557:CDS	35.0%
	GAGAAGAAGATCTCAAAAGA+AGG	+	chr7.4:10297594-10297613	MS.gene018557:CDS	35.0%
	GATTCAAACTGCTGTTAGAT+TGG	+	chr7.4:10297833-10297852	MS.gene018557:CDS	35.0%
	TTGCTTGAAACACCAATATC+AGG	-	chr7.4:10297705-10297724	None:intergenic	35.0%
!	TATCTTTGAGAAACTTGCTC+AGG	+	chr7.4:10297758-10297777	MS.gene018557:CDS	35.0%
!	TGATATTGGTGTTTCAAGCA+AGG	+	chr7.4:10297704-10297723	MS.gene018557:CDS	35.0%
!!	CTAAACATGCTGTTTCTGAA+GGG	+	chr7.4:10297874-10297893	MS.gene018557:CDS	35.0%
!	ATTGGTTCTTCCTGGAGAAT+TGG	+	chr7.4:10297851-10297870	MS.gene018557:CDS	40.0%
!	GGTTTCTTTTCTGCCTTTGT+GGG	-	chr7.4:10297522-10297541	None:intergenic	40.0%
!!	CATCTTCAAGGTTCTGAAAC+AGG	+	chr7.4:10297674-10297693	MS.gene018557:CDS	40.0%
!!	GCTAAACATGCTGTTTCTGA+AGG	+	chr7.4:10297873-10297892	MS.gene018557:CDS	40.0%
	AAAGGCAGAAAAGAAACCCG+CGG	+	chr7.4:10297524-10297543	MS.gene018557:CDS	45.0%
	ATCTCCCTTGAAGTGATCGT+CGG	-	chr7.4:10297816-10297835	None:intergenic	45.0%
	GCATGTTTAGCCAATTCTCC+AGG	-	chr7.4:10297864-10297883	None:intergenic	45.0%
	GGTGTTTCAAGCAAGGCTAT+GGG	+	chr7.4:10297711-10297730	MS.gene018557:CDS	45.0%
	TGGTGTTTCAAGCAAGGCTA+TGG	+	chr7.4:10297710-10297729	MS.gene018557:CDS	45.0%
!	GCTGTTAGATTGGTTCTTCC+TGG	+	chr7.4:10297843-10297862	MS.gene018557:CDS	45.0%
!	GGGTTTCTTTTCTGCCTTTG+TGG	-	chr7.4:10297523-10297542	None:intergenic	45.0%
!!	TGCTGTTTCTGAAGGGACTA+AGG	+	chr7.4:10297881-10297900	MS.gene018557:CDS	45.0%
	AGAAGCCGACGATCACTTCA+AGG	+	chr7.4:10297808-10297827	MS.gene018557:CDS	50.0%
	AGCCGAGAAGAAACCAAAGG+CGG	+	chr7.4:10297572-10297591	MS.gene018557:CDS	50.0%
	AGGAATCGTCTCGTTTGGCT+AGG	+	chr7.4:10297778-10297797	MS.gene018557:CDS	50.0%
	GAAGCCGACGATCACTTCAA+GGG	+	chr7.4:10297809-10297828	MS.gene018557:CDS	50.0%
	GAGATCTTCTTCTCCGCCTT+TGG	-	chr7.4:10297588-10297607	None:intergenic	50.0%
	TCCAGCCGAGAAGAAACCAA+AGG	+	chr7.4:10297569-10297588	MS.gene018557:CDS	50.0%
	TGCTCAGGAATCGTCTCGTT+TGG	+	chr7.4:10297773-10297792	MS.gene018557:CDS	50.0%
!	CTCCGCCTTTGGTTTCTTCT+CGG	-	chr7.4:10297577-10297596	None:intergenic	50.0%
!	GCCTTTGGTTTCTTCTCGGC+TGG	-	chr7.4:10297573-10297592	None:intergenic	55.0%
	CTCGGCTGGAGCTTTCTCTG+CGG	-	chr7.4:10297559-10297578	None:intergenic	60.0%
	TCGGCTGGAGCTTTCTCTGC+GGG	-	chr7.4:10297558-10297577	None:intergenic	60.0%
	CTCTGCGGGCTTCTTCTCCG+CGG	-	chr7.4:10297544-10297563	None:intergenic	65.0%
	TCTGCGGGCTTCTTCTCCGC+GGG	-	chr7.4:10297543-10297562	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	10297513	10297929	10297513	ID=MS.gene018557
chr7.4	mRNA	10297513	10297929	10297513	ID=MS.gene018557.t1;Parent=MS.gene018557
chr7.4	exon	10297513	10297929	10297513	ID=MS.gene018557.t1.exon1;Parent=MS.gene018557.t1
chr7.4	CDS	10297513	10297929	10297513	ID=cds.MS.gene018557.t1;Parent=MS.gene018557.t1

Gene Sequence

>MS.gene018557

ATGGCTCCCACAAAGGCAGAAAAGAAACCCGCGGAGAAGAAGCCCGCAGAGAAAGCTCCAGCCGAGAAGAAACCAAAGGCGGAGAAGAAGATCTCAAAAGAAGGAAGCAGCGACAAGAAGAAAAAGAGAACGAAGAAAAGCGTTGAAACATACAAGATATACATCTTCAAGGTTCTGAAACAGGTTCATCCTGATATTGGTGTTTCAAGCAAGGCTATGGGAATTATGAACAGTTTCATTAACGATATCTTTGAGAAACTTGCTCAGGAATCGTCTCGTTTGGCTAGGTATAACAAGAAGCCGACGATCACTTCAAGGGAGATTCAAACTGCTGTTAGATTGGTTCTTCCTGGAGAATTGGCTAAACATGCTGTTTCTGAAGGGACTAAGGCTGTTACCAAATTCACCAGCTCTTGA

Protein sequence

>MS.gene018557.t1

MAPTKAEKKPAEKKPAEKAPAEKKPKAEKKISKEGSSDKKKKRTKKSVETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS