Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018558.t1 | XP_024625861.1 | 92.8 | 251 | 18 | 0 | 1 | 251 | 1 | 251 | 1.40E-103 | 386 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018558.t1 | G7L0L2 | 92.8 | 251 | 18 | 0 | 1 | 251 | 85 | 335 | 9.9e-104 | 386.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene073852 | MS.gene018558 | PPI |
| MS.gene64517 | MS.gene018558 | PPI |
| MS.gene53776 | MS.gene018558 | PPI |
| MS.gene018558 | MS.gene068527 | PPI |
| MS.gene018558 | MS.gene058978 | PPI |
| MS.gene24068 | MS.gene018558 | PPI |
| MS.gene061900 | MS.gene018558 | PPI |
| MS.gene09850 | MS.gene018558 | PPI |
| MS.gene058978 | MS.gene018558 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018558.t1 | MTR_7g099950 | 92.829 | 251 | 18 | 0 | 1 | 251 | 85 | 335 | 1.19e-141 | 400 |
| MS.gene018558.t1 | MTR_1g068825 | 44.922 | 256 | 124 | 5 | 1 | 251 | 1 | 244 | 7.60e-47 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018558.t1 | AT2G37480 | 40.336 | 119 | 48 | 4 | 81 | 194 | 71 | 171 | 1.77e-13 | 67.4 |
| MS.gene018558.t1 | AT3G53670 | 36.800 | 125 | 71 | 3 | 69 | 193 | 61 | 177 | 2.03e-13 | 67.4 |
| MS.gene018558.t1 | AT3G53670 | 36.800 | 125 | 71 | 3 | 69 | 193 | 61 | 177 | 2.03e-13 | 67.4 |
| MS.gene018558.t1 | AT2G37480 | 40.336 | 119 | 49 | 3 | 81 | 194 | 71 | 172 | 3.80e-13 | 66.6 |
Find 42 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCCGCAGTTTGAATCT+TGG | 0.342574 | 7.4:-10305994 | None:intergenic |
| GGCAACGTCGCATGAGGAAT+TGG | 0.368193 | 7.4:-10305202 | None:intergenic |
| ATAGGAAATTGACGGTGAAA+TGG | 0.412052 | 7.4:+10305714 | MS.gene018558:CDS |
| TTTATCACGGCTACCTTTCA+AGG | 0.425025 | 7.4:-10305678 | None:intergenic |
| ACAAGAAACAGCCGAAATCC+AGG | 0.447680 | 7.4:+10305789 | MS.gene018558:CDS |
| TAGGAAATTGACGGTGAAAT+GGG | 0.449608 | 7.4:+10305715 | MS.gene018558:CDS |
| TGGGATTCTCAAGTTATTGA+AGG | 0.461070 | 7.4:+10305938 | MS.gene018558:CDS |
| AAGCAGTCTCGCAACGTTGG+TGG | 0.501681 | 7.4:+10305890 | MS.gene018558:CDS |
| TCCTCGTCAGAAGAGCTTGA+AGG | 0.508539 | 7.4:-10305223 | None:intergenic |
| AAGGCAGATTTGAGCTCCGA+AGG | 0.526871 | 7.4:-10305659 | None:intergenic |
| AGCAGTCTCGCAACGTTGGT+GGG | 0.540201 | 7.4:+10305891 | MS.gene018558:CDS |
| GCCTTCAAGCTCTTCTGACG+AGG | 0.541579 | 7.4:+10305222 | MS.gene018558:CDS |
| TGCGACACTAGTGTAGGTAT+AGG | 0.544332 | 7.4:-10305755 | None:intergenic |
| TTCACCGTCAATTTCCTATG+TGG | 0.544822 | 7.4:-10305710 | None:intergenic |
| AAATGTAAAGAAAGGTAACA+AGG | 0.560400 | 7.4:+10305832 | MS.gene018558:CDS |
| AATGTAAAGAAAGGTAACAA+GGG | 0.568123 | 7.4:+10305833 | MS.gene018558:CDS |
| GAGCTCAAATCTGCCTTGAA+AGG | 0.568787 | 7.4:+10305665 | MS.gene018558:CDS |
| GAAATCTGAACATTTGTCTG+AGG | 0.568873 | 7.4:+10305078 | MS.gene018558:CDS |
| AAATCTGAACATTTGTCTGA+GGG | 0.573710 | 7.4:+10305079 | MS.gene018558:CDS |
| TGAACCACATAGGAAATTGA+CGG | 0.581672 | 7.4:+10305706 | MS.gene018558:CDS |
| CTGATCTGTGCAGTAAGTCG+TGG | 0.587620 | 7.4:+10305918 | MS.gene018558:CDS |
| ATCTGAACATTTGTCTGAGG+GGG | 0.595296 | 7.4:+10305081 | MS.gene018558:CDS |
| CTTGAAGGCAACGTCGCATG+AGG | 0.604027 | 7.4:-10305208 | None:intergenic |
| AGAAGTGCATTACTCAGTTG+CGG | 0.604827 | 7.4:+10306039 | MS.gene018558:CDS |
| TGATCTGTGCAGTAAGTCGT+GGG | 0.608135 | 7.4:+10305919 | MS.gene018558:CDS |
| TAGCCAAGGAAAACTCAAGT+GGG | 0.608660 | 7.4:+10305119 | MS.gene018558:CDS |
| AAGAAGCAGTCTCGCAACGT+TGG | 0.609694 | 7.4:+10305887 | MS.gene018558:CDS |
| TGCGATGTGCAATTTAGCCA+AGG | 0.611431 | 7.4:+10305105 | MS.gene018558:CDS |
| ATCAAGAGAGTCAAATTCAG+AGG | 0.614512 | 7.4:-10305963 | None:intergenic |
| ATGTAAAGAAAGGTAACAAG+GGG | 0.626414 | 7.4:+10305834 | MS.gene018558:CDS |
| TGATGAGCAGAATGCTCATG+AGG | 0.641178 | 7.4:-10305271 | None:intergenic |
| AGCCAAGGAAAACTCAAGTG+GGG | 0.641950 | 7.4:+10305120 | MS.gene018558:CDS |
| AAGGGGAATTCAACCCGAGG+GGG | 0.642568 | 7.4:+10305851 | MS.gene018558:CDS |
| GAAAGAATCAAAACCCAACA+TGG | 0.643797 | 7.4:+10305153 | MS.gene018558:CDS |
| CGTCGCATGAGGAATTGGAA+AGG | 0.653621 | 7.4:-10305197 | None:intergenic |
| ACAGTGTGCGACACTAGTGT+AGG | 0.663479 | 7.4:-10305761 | None:intergenic |
| TGTAGCCAAGATTCAAACTG+CGG | 0.664125 | 7.4:+10305989 | MS.gene018558:CDS |
| TTAGCCAAGGAAAACTCAAG+TGG | 0.667603 | 7.4:+10305118 | MS.gene018558:CDS |
| AATCTGAACATTTGTCTGAG+GGG | 0.684222 | 7.4:+10305080 | MS.gene018558:CDS |
| AACAAGGGGAATTCAACCCG+AGG | 0.692269 | 7.4:+10305848 | MS.gene018558:CDS |
| ACAAGGGGAATTCAACCCGA+GGG | 0.697575 | 7.4:+10305849 | MS.gene018558:CDS |
| CAAGGGGAATTCAACCCGAG+GGG | 0.745728 | 7.4:+10305850 | MS.gene018558:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTTAACACTATATTGAAT+AGG | - | chr7.4:10305494-10305513 | None:intergenic | 20.0% |
| !! | ATTGAATAGGTAAATTCAAT+AGG | - | chr7.4:10305481-10305500 | None:intergenic | 20.0% |
| !! | GAAAAGAAAAATGTAAAGAA+AGG | + | chr7.4:10305824-10305843 | MS.gene018558:CDS | 20.0% |
| !! | GATGTAAAATACATAGAAAA+AGG | - | chr7.4:10305632-10305651 | None:intergenic | 20.0% |
| !! | TCAATATAGTGTTAAACTAA+TGG | + | chr7.4:10305496-10305515 | MS.gene018558:intron | 20.0% |
| ! | AAATGTAAAGAAAGGTAACA+AGG | + | chr7.4:10305832-10305851 | MS.gene018558:CDS | 25.0% |
| ! | AATGTAAAGAAAGGTAACAA+GGG | + | chr7.4:10305833-10305852 | MS.gene018558:CDS | 25.0% |
| ! | TTAATGATGCTTATGTGTAT+TGG | + | chr7.4:10305350-10305369 | MS.gene018558:intron | 25.0% |
| ! | TTGTAACATGAAAAACTTAC+AGG | - | chr7.4:10305322-10305341 | None:intergenic | 25.0% |
| !! | AGTTTTTCATGTTACAAAGT+AGG | + | chr7.4:10305325-10305344 | MS.gene018558:intron | 25.0% |
| !! | TTCTATGTATTTTACATCAC+AGG | + | chr7.4:10305634-10305653 | MS.gene018558:intron | 25.0% |
| !!! | AATTTCTGTTTTGAAAAAGC+TGG | + | chr7.4:10305394-10305413 | MS.gene018558:intron | 25.0% |
| AAATCTGAACATTTGTCTGA+GGG | + | chr7.4:10305079-10305098 | MS.gene018558:CDS | 30.0% | |
| ATGTAAAGAAAGGTAACAAG+GGG | + | chr7.4:10305834-10305853 | MS.gene018558:CDS | 30.0% | |
| CAACAAATCAATACCACATA+TGG | - | chr7.4:10305427-10305446 | None:intergenic | 30.0% | |
| TGTCCGAATAACAAAAAGAA+CGG | - | chr7.4:10305533-10305552 | None:intergenic | 30.0% | |
| ! | CTTTTCATTCTTCTTTTTCC+TGG | - | chr7.4:10305810-10305829 | None:intergenic | 30.0% |
| ! | TTCTTCTTTTTCCTGGATTT+CGG | - | chr7.4:10305803-10305822 | None:intergenic | 30.0% |
| !! | CATATGTGGTATTGATTTGT+TGG | + | chr7.4:10305425-10305444 | MS.gene018558:intron | 30.0% |
| !! | TTTTTCAAACACACCATGTT+GGG | - | chr7.4:10305169-10305188 | None:intergenic | 30.0% |
| !!! | ACACCGTTCTTTTTGTTATT+CGG | + | chr7.4:10305527-10305546 | MS.gene018558:intron | 30.0% |
| !!! | TTTTTTCAAACACACCATGT+TGG | - | chr7.4:10305170-10305189 | None:intergenic | 30.0% |
| AAAGGTAGCCGTGATAAAAA+CGG | + | chr7.4:10305683-10305702 | MS.gene018558:CDS | 35.0% | |
| AATCTGAACATTTGTCTGAG+GGG | + | chr7.4:10305080-10305099 | MS.gene018558:CDS | 35.0% | |
| AGCTGTTGAAAAAAATGCAC+AGG | - | chr7.4:10305608-10305627 | None:intergenic | 35.0% | |
| ATAAAAACGGTGAACCACAT+AGG | + | chr7.4:10305696-10305715 | MS.gene018558:CDS | 35.0% | |
| ATAGGAAATTGACGGTGAAA+TGG | + | chr7.4:10305714-10305733 | MS.gene018558:CDS | 35.0% | |
| ATCAAGAGAGTCAAATTCAG+AGG | - | chr7.4:10305966-10305985 | None:intergenic | 35.0% | |
| CAATAGGAATGCTTAACAGA+TGG | - | chr7.4:10305465-10305484 | None:intergenic | 35.0% | |
| GAAAGAATCAAAACCCAACA+TGG | + | chr7.4:10305153-10305172 | MS.gene018558:CDS | 35.0% | |
| GAAATCTGAACATTTGTCTG+AGG | + | chr7.4:10305078-10305097 | MS.gene018558:CDS | 35.0% | |
| TAGGAAATTGACGGTGAAAT+GGG | + | chr7.4:10305715-10305734 | MS.gene018558:CDS | 35.0% | |
| TGAACCACATAGGAAATTGA+CGG | + | chr7.4:10305706-10305725 | MS.gene018558:CDS | 35.0% | |
| TGGGATTCTCAAGTTATTGA+AGG | + | chr7.4:10305938-10305957 | MS.gene018558:CDS | 35.0% | |
| ATCTGAACATTTGTCTGAGG+GGG | + | chr7.4:10305081-10305100 | MS.gene018558:CDS | 40.0% | |
| TAGCCAAGGAAAACTCAAGT+GGG | + | chr7.4:10305119-10305138 | MS.gene018558:CDS | 40.0% | |
| TGTAGCCAAGATTCAAACTG+CGG | + | chr7.4:10305989-10306008 | MS.gene018558:CDS | 40.0% | |
| TTAGCCAAGGAAAACTCAAG+TGG | + | chr7.4:10305118-10305137 | MS.gene018558:CDS | 40.0% | |
| TTCACCGTCAATTTCCTATG+TGG | - | chr7.4:10305713-10305732 | None:intergenic | 40.0% | |
| TTTATCACGGCTACCTTTCA+AGG | - | chr7.4:10305681-10305700 | None:intergenic | 40.0% | |
| ! | AGAAGTGCATTACTCAGTTG+CGG | + | chr7.4:10306039-10306058 | MS.gene018558:CDS | 40.0% |
| ! | TTTTACATCACAGGCTCCTT+CGG | + | chr7.4:10305643-10305662 | MS.gene018558:intron | 40.0% |
| !! | GTGGTTCACCGTTTTTATCA+CGG | - | chr7.4:10305694-10305713 | None:intergenic | 40.0% |
| ACAAGAAACAGCCGAAATCC+AGG | + | chr7.4:10305789-10305808 | MS.gene018558:CDS | 45.0% | |
| AGCCAAGGAAAACTCAAGTG+GGG | + | chr7.4:10305120-10305139 | MS.gene018558:CDS | 45.0% | |
| AGCTGGCGTTATTCCATATG+TGG | + | chr7.4:10305411-10305430 | MS.gene018558:intron | 45.0% | |
| GAGCTCAAATCTGCCTTGAA+AGG | + | chr7.4:10305665-10305684 | MS.gene018558:CDS | 45.0% | |
| TGATCTGTGCAGTAAGTCGT+GGG | + | chr7.4:10305919-10305938 | MS.gene018558:CDS | 45.0% | |
| TGATGAGCAGAATGCTCATG+AGG | - | chr7.4:10305274-10305293 | None:intergenic | 45.0% | |
| TGCGACACTAGTGTAGGTAT+AGG | - | chr7.4:10305758-10305777 | None:intergenic | 45.0% | |
| TGCGATGTGCAATTTAGCCA+AGG | + | chr7.4:10305105-10305124 | MS.gene018558:CDS | 45.0% | |
| TGCTTCCGCAGTTTGAATCT+TGG | - | chr7.4:10305997-10306016 | None:intergenic | 45.0% | |
| ! | AACCCCACTTGAGTTTTCCT+TGG | - | chr7.4:10305125-10305144 | None:intergenic | 45.0% |
| AACAAGGGGAATTCAACCCG+AGG | + | chr7.4:10305848-10305867 | MS.gene018558:CDS | 50.0% | |
| AAGAAGCAGTCTCGCAACGT+TGG | + | chr7.4:10305887-10305906 | MS.gene018558:CDS | 50.0% | |
| AAGGCAGATTTGAGCTCCGA+AGG | - | chr7.4:10305662-10305681 | None:intergenic | 50.0% | |
| ACAAGGGGAATTCAACCCGA+GGG | + | chr7.4:10305849-10305868 | MS.gene018558:CDS | 50.0% | |
| ACAGTGTGCGACACTAGTGT+AGG | - | chr7.4:10305764-10305783 | None:intergenic | 50.0% | |
| CGTCGCATGAGGAATTGGAA+AGG | - | chr7.4:10305200-10305219 | None:intergenic | 50.0% | |
| CTGATCTGTGCAGTAAGTCG+TGG | + | chr7.4:10305918-10305937 | MS.gene018558:CDS | 50.0% | |
| TATCTTTGCCATTGCCCCCT+CGG | - | chr7.4:10305868-10305887 | None:intergenic | 50.0% | |
| TCCTCGTCAGAAGAGCTTGA+AGG | - | chr7.4:10305226-10305245 | None:intergenic | 50.0% | |
| AAGCAGTCTCGCAACGTTGG+TGG | + | chr7.4:10305890-10305909 | MS.gene018558:CDS | 55.0% | |
| AAGGGGAATTCAACCCGAGG+GGG | + | chr7.4:10305851-10305870 | MS.gene018558:CDS | 55.0% | |
| AATTCAACCCGAGGGGGCAA+TGG | + | chr7.4:10305857-10305876 | MS.gene018558:CDS | 55.0% | |
| AGCAGTCTCGCAACGTTGGT+GGG | + | chr7.4:10305891-10305910 | MS.gene018558:CDS | 55.0% | |
| ATCTTTGCCATTGCCCCCTC+GGG | - | chr7.4:10305867-10305886 | None:intergenic | 55.0% | |
| CAAGGGGAATTCAACCCGAG+GGG | + | chr7.4:10305850-10305869 | MS.gene018558:CDS | 55.0% | |
| CTTGAAGGCAACGTCGCATG+AGG | - | chr7.4:10305211-10305230 | None:intergenic | 55.0% | |
| GCCTTCAAGCTCTTCTGACG+AGG | + | chr7.4:10305222-10305241 | MS.gene018558:CDS | 55.0% | |
| GGCAACGTCGCATGAGGAAT+TGG | - | chr7.4:10305205-10305224 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10304983 | 10306072 | 10304983 | ID=MS.gene018558 |
| chr7.4 | mRNA | 10304983 | 10306072 | 10304983 | ID=MS.gene018558.t1;Parent=MS.gene018558 |
| chr7.4 | exon | 10304983 | 10305321 | 10304983 | ID=MS.gene018558.t1.exon1;Parent=MS.gene018558.t1 |
| chr7.4 | CDS | 10304983 | 10305321 | 10304983 | ID=cds.MS.gene018558.t1;Parent=MS.gene018558.t1 |
| chr7.4 | exon | 10305656 | 10306072 | 10305656 | ID=MS.gene018558.t1.exon2;Parent=MS.gene018558.t1 |
| chr7.4 | CDS | 10305656 | 10306072 | 10305656 | ID=cds.MS.gene018558.t1;Parent=MS.gene018558.t1 |
| Gene Sequence |
| Protein sequence |
