Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061900.t1 | XP_024625861.1 | 92.4 | 251 | 19 | 0 | 1 | 251 | 1 | 251 | 3.10E-103 | 384.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061900.t1 | G7L0L2 | 92.4 | 251 | 19 | 0 | 1 | 251 | 85 | 335 | 2.2e-103 | 384.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene061900 | 0.840998 | 6.20E-58 | -1.69E-46 |
| MS.gene049873 | MS.gene061900 | 0.843255 | 1.57E-58 | -1.69E-46 |
| MS.gene050303 | MS.gene061900 | 0.807951 | 3.93E-50 | -1.69E-46 |
| MS.gene050799 | MS.gene061900 | 0.821938 | 3.09E-53 | -1.69E-46 |
| MS.gene051678 | MS.gene061900 | 0.830234 | 3.29E-55 | -1.69E-46 |
| MS.gene052208 | MS.gene061900 | 0.805493 | 1.30E-49 | -1.69E-46 |
| MS.gene052286 | MS.gene061900 | 0.813709 | 2.23E-51 | -1.69E-46 |
| MS.gene052358 | MS.gene061900 | -0.866651 | 2.44E-65 | -1.69E-46 |
| MS.gene052361 | MS.gene061900 | -0.801852 | 7.40E-49 | -1.69E-46 |
| MS.gene052458 | MS.gene061900 | 0.811328 | 7.40E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene061900 | 0.81536 | 9.62E-52 | -1.69E-46 |
| MS.gene053006 | MS.gene061900 | 0.817637 | 2.97E-52 | -1.69E-46 |
| MS.gene053294 | MS.gene061900 | 0.858695 | 6.90E-63 | -1.69E-46 |
| MS.gene053401 | MS.gene061900 | 0.819901 | 9.10E-53 | -1.69E-46 |
| MS.gene053900 | MS.gene061900 | 0.832433 | 9.48E-56 | -1.69E-46 |
| MS.gene053995 | MS.gene061900 | 0.815507 | 8.92E-52 | -1.69E-46 |
| MS.gene055374 | MS.gene061900 | 0.861566 | 9.38E-64 | -1.69E-46 |
| MS.gene05548 | MS.gene061900 | 0.856089 | 4.07E-62 | -1.69E-46 |
| MS.gene055555 | MS.gene061900 | 0.826956 | 2.04E-54 | -1.69E-46 |
| MS.gene055808 | MS.gene061900 | 0.81957 | 1.08E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene061900 | 0.805341 | 1.40E-49 | -1.69E-46 |
| MS.gene056091 | MS.gene061900 | 0.843969 | 1.01E-58 | -1.69E-46 |
| MS.gene056642 | MS.gene061900 | 0.814007 | 1.92E-51 | -1.69E-46 |
| MS.gene057060 | MS.gene061900 | 0.811253 | 7.68E-51 | -1.69E-46 |
| MS.gene057079 | MS.gene061900 | 0.809805 | 1.58E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene061900 | 0.815265 | 1.01E-51 | -1.69E-46 |
| MS.gene05795 | MS.gene061900 | 0.8222 | 2.69E-53 | -1.69E-46 |
| MS.gene058037 | MS.gene061900 | 0.827712 | 1.34E-54 | -1.69E-46 |
| MS.gene058635 | MS.gene061900 | 0.819286 | 1.26E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene061900 | 0.833366 | 5.56E-56 | -1.69E-46 |
| MS.gene060572 | MS.gene061900 | 0.819531 | 1.11E-52 | -1.69E-46 |
| MS.gene060917 | MS.gene061900 | 0.817265 | 3.61E-52 | -1.69E-46 |
| MS.gene061040 | MS.gene061900 | -0.814569 | 1.44E-51 | -1.69E-46 |
| MS.gene061266 | MS.gene061900 | 0.830123 | 3.51E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene061900 | MS.gene09850 | PPI |
| MS.gene061900 | MS.gene64517 | PPI |
| MS.gene09850 | MS.gene061900 | PPI |
| MS.gene061900 | MS.gene068527 | PPI |
| MS.gene061900 | MS.gene058978 | PPI |
| MS.gene061900 | MS.gene018558 | PPI |
| MS.gene061900 | MS.gene073852 | PPI |
| MS.gene061900 | MS.gene53776 | PPI |
| MS.gene061900 | MS.gene24068 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061900.t1 | MTR_7g099950 | 92.430 | 251 | 19 | 0 | 1 | 251 | 85 | 335 | 2.96e-140 | 397 |
| MS.gene061900.t1 | MTR_1g068825 | 44.531 | 256 | 125 | 5 | 1 | 251 | 1 | 244 | 2.95e-46 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061900.t1 | AT2G37480 | 40.336 | 119 | 48 | 4 | 81 | 194 | 71 | 171 | 2.49e-13 | 67.0 |
| MS.gene061900.t1 | AT3G53670 | 36.800 | 125 | 71 | 2 | 69 | 193 | 61 | 177 | 3.36e-13 | 67.0 |
| MS.gene061900.t1 | AT3G53670 | 36.800 | 125 | 71 | 2 | 69 | 193 | 61 | 177 | 3.36e-13 | 67.0 |
| MS.gene061900.t1 | AT2G37480 | 40.336 | 119 | 49 | 3 | 81 | 194 | 71 | 172 | 4.69e-13 | 66.2 |
Find 42 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCCGCAGTTTGAATCT+TGG | 0.342574 | 7.2:-12035422 | None:intergenic |
| GGCAACGTCGCATGAGGAAT+TGG | 0.368193 | 7.2:-12034630 | None:intergenic |
| ATAGGAAATTGACGGTGAAA+TGG | 0.412052 | 7.2:+12035142 | MS.gene061900:CDS |
| TTTATCACGGCTACCTTTCA+AGG | 0.425025 | 7.2:-12035106 | None:intergenic |
| ACAAGAAACAGCCGAAATCC+AGG | 0.447680 | 7.2:+12035217 | MS.gene061900:CDS |
| TAGGAAATTGACGGTGAAAT+GGG | 0.449608 | 7.2:+12035143 | MS.gene061900:CDS |
| TGGGATTCTCAAGTTATTGA+AGG | 0.461070 | 7.2:+12035366 | MS.gene061900:CDS |
| AAGCAGTCTCGCAACGTTGG+TGG | 0.501681 | 7.2:+12035318 | MS.gene061900:CDS |
| TCCTCGTCAGAAGAGCTTGA+AGG | 0.508539 | 7.2:-12034651 | None:intergenic |
| AAGGCAGATTTGAGCTCCGA+AGG | 0.526871 | 7.2:-12035087 | None:intergenic |
| AGCAGTCTCGCAACGTTGGT+GGG | 0.540201 | 7.2:+12035319 | MS.gene061900:CDS |
| GCCTTCAAGCTCTTCTGACG+AGG | 0.541579 | 7.2:+12034650 | MS.gene061900:CDS |
| TGCGACACTAGTGTAGGTAT+AGG | 0.544332 | 7.2:-12035183 | None:intergenic |
| TTCACCGTCAATTTCCTATG+TGG | 0.544822 | 7.2:-12035138 | None:intergenic |
| AAATGTAAAGAAAGGTAACA+AGG | 0.560400 | 7.2:+12035260 | MS.gene061900:CDS |
| AATGTAAAGAAAGGTAACAA+GGG | 0.568123 | 7.2:+12035261 | MS.gene061900:CDS |
| GAGCTCAAATCTGCCTTGAA+AGG | 0.568787 | 7.2:+12035093 | MS.gene061900:CDS |
| GAAATCTGAACATTTGTCTG+AGG | 0.568873 | 7.2:+12034506 | MS.gene061900:CDS |
| AAATCTGAACATTTGTCTGA+GGG | 0.573710 | 7.2:+12034507 | MS.gene061900:CDS |
| TGAACCACATAGGAAATTGA+CGG | 0.581672 | 7.2:+12035134 | MS.gene061900:CDS |
| CTGATCTGTGCAGTAAGTCG+TGG | 0.587620 | 7.2:+12035346 | MS.gene061900:CDS |
| TGCGATGTGCAATGTAGCCA+AGG | 0.589787 | 7.2:+12034533 | MS.gene061900:CDS |
| ATCTGAACATTTGTCTGAGG+GGG | 0.595296 | 7.2:+12034509 | MS.gene061900:CDS |
| CTTGAAGGCAACGTCGCATG+AGG | 0.604027 | 7.2:-12034636 | None:intergenic |
| AGAAGTGCATTACTCAGTTG+CGG | 0.604827 | 7.2:+12035467 | MS.gene061900:CDS |
| TGATCTGTGCAGTAAGTCGT+GGG | 0.608135 | 7.2:+12035347 | MS.gene061900:CDS |
| AAGAAGCAGTCTCGCAACGT+TGG | 0.609694 | 7.2:+12035315 | MS.gene061900:CDS |
| TAGCCAAGGAAAACTCAAGT+GGG | 0.611731 | 7.2:+12034547 | MS.gene061900:CDS |
| ATCAAGAGAGTCAAATTCAG+AGG | 0.614512 | 7.2:-12035391 | None:intergenic |
| ATGTAAAGAAAGGTAACAAG+GGG | 0.626414 | 7.2:+12035262 | MS.gene061900:CDS |
| TGATGAGCAGAATGCTCATG+AGG | 0.629399 | 7.2:-12034699 | None:intergenic |
| AAGGGGAATTCAACCCGAGG+GGG | 0.642568 | 7.2:+12035279 | MS.gene061900:CDS |
| GAAAGAATCAAAACCCAACA+TGG | 0.643797 | 7.2:+12034581 | MS.gene061900:CDS |
| CGTCGCATGAGGAATTGGAA+AGG | 0.653621 | 7.2:-12034625 | None:intergenic |
| ACAGTGTGCGACACTAGTGT+AGG | 0.663479 | 7.2:-12035189 | None:intergenic |
| TGTAGCCAAGATTCAAACTG+CGG | 0.664125 | 7.2:+12035417 | MS.gene061900:CDS |
| GTAGCCAAGGAAAACTCAAG+TGG | 0.667603 | 7.2:+12034546 | MS.gene061900:CDS |
| AATCTGAACATTTGTCTGAG+GGG | 0.684222 | 7.2:+12034508 | MS.gene061900:CDS |
| AACAAGGGGAATTCAACCCG+AGG | 0.692269 | 7.2:+12035276 | MS.gene061900:CDS |
| ACAAGGGGAATTCAACCCGA+GGG | 0.697575 | 7.2:+12035277 | MS.gene061900:CDS |
| AGCCAAGGAAAACTCAAGTG+GGG | 0.705918 | 7.2:+12034548 | MS.gene061900:CDS |
| CAAGGGGAATTCAACCCGAG+GGG | 0.745728 | 7.2:+12035278 | MS.gene061900:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGGCTAAATTAAAAAAAAAA+CGG | - | chr7.2:12035016-12035035 | None:intergenic | 15.0% |
| !! | AGTTTAACACTATATTGAAT+AGG | - | chr7.2:12034922-12034941 | None:intergenic | 20.0% |
| !! | ATTGAATAGGTAAATTCAAT+AGG | - | chr7.2:12034909-12034928 | None:intergenic | 20.0% |
| !! | GAAAAGAAAAATGTAAAGAA+AGG | + | chr7.2:12035252-12035271 | MS.gene061900:CDS | 20.0% |
| !! | GATGTAAAATACATAGAAAA+AGG | - | chr7.2:12035060-12035079 | None:intergenic | 20.0% |
| !! | TCAATATAGTGTTAAACTAA+TGG | + | chr7.2:12034924-12034943 | MS.gene061900:intron | 20.0% |
| ! | AAATGTAAAGAAAGGTAACA+AGG | + | chr7.2:12035260-12035279 | MS.gene061900:CDS | 25.0% |
| ! | AATGTAAAGAAAGGTAACAA+GGG | + | chr7.2:12035261-12035280 | MS.gene061900:CDS | 25.0% |
| ! | TTAATGATGCTTATGTGTAT+TGG | + | chr7.2:12034778-12034797 | MS.gene061900:intron | 25.0% |
| ! | TTGTAACATGAAAAACTTAC+AGG | - | chr7.2:12034750-12034769 | None:intergenic | 25.0% |
| !! | AGTTTTTCATGTTACAAAGT+CGG | + | chr7.2:12034753-12034772 | MS.gene061900:intron | 25.0% |
| !! | TTCTATGTATTTTACATCAC+AGG | + | chr7.2:12035062-12035081 | MS.gene061900:intron | 25.0% |
| !!! | AATTTCTGTTTTGAAAAAGC+CGG | + | chr7.2:12034822-12034841 | MS.gene061900:intron | 25.0% |
| AAATCTGAACATTTGTCTGA+GGG | + | chr7.2:12034507-12034526 | MS.gene061900:CDS | 30.0% | |
| ATGTAAAGAAAGGTAACAAG+GGG | + | chr7.2:12035262-12035281 | MS.gene061900:CDS | 30.0% | |
| CAACAAATCAATACCACATA+TGG | - | chr7.2:12034855-12034874 | None:intergenic | 30.0% | |
| TGTCCGAATAACAAAAAGAA+CGG | - | chr7.2:12034961-12034980 | None:intergenic | 30.0% | |
| ! | CTTTTCATTCTTCTTTTTCC+TGG | - | chr7.2:12035238-12035257 | None:intergenic | 30.0% |
| ! | TTCTTCTTTTTCCTGGATTT+CGG | - | chr7.2:12035231-12035250 | None:intergenic | 30.0% |
| !! | CATATGTGGTATTGATTTGT+TGG | + | chr7.2:12034853-12034872 | MS.gene061900:intron | 30.0% |
| !! | TTTTTCAAACACACCATGTT+GGG | - | chr7.2:12034597-12034616 | None:intergenic | 30.0% |
| !!! | ACACCGTTCTTTTTGTTATT+CGG | + | chr7.2:12034955-12034974 | MS.gene061900:intron | 30.0% |
| !!! | TTTTTTCAAACACACCATGT+TGG | - | chr7.2:12034598-12034617 | None:intergenic | 30.0% |
| AAAGGTAGCCGTGATAAAAA+CGG | + | chr7.2:12035111-12035130 | MS.gene061900:CDS | 35.0% | |
| AATCTGAACATTTGTCTGAG+GGG | + | chr7.2:12034508-12034527 | MS.gene061900:CDS | 35.0% | |
| AGCTGTTGAAAAAAATGCAC+AGG | - | chr7.2:12035036-12035055 | None:intergenic | 35.0% | |
| ATAAAAACGGTGAACCACAT+AGG | + | chr7.2:12035124-12035143 | MS.gene061900:CDS | 35.0% | |
| ATAGGAAATTGACGGTGAAA+TGG | + | chr7.2:12035142-12035161 | MS.gene061900:CDS | 35.0% | |
| ATCAAGAGAGTCAAATTCAG+AGG | - | chr7.2:12035394-12035413 | None:intergenic | 35.0% | |
| CAATAGGAATGCTTAACAGA+TGG | - | chr7.2:12034893-12034912 | None:intergenic | 35.0% | |
| GAAAGAATCAAAACCCAACA+TGG | + | chr7.2:12034581-12034600 | MS.gene061900:CDS | 35.0% | |
| GAAATCTGAACATTTGTCTG+AGG | + | chr7.2:12034506-12034525 | MS.gene061900:CDS | 35.0% | |
| TAGGAAATTGACGGTGAAAT+GGG | + | chr7.2:12035143-12035162 | MS.gene061900:CDS | 35.0% | |
| TGAACCACATAGGAAATTGA+CGG | + | chr7.2:12035134-12035153 | MS.gene061900:CDS | 35.0% | |
| TGGGATTCTCAAGTTATTGA+AGG | + | chr7.2:12035366-12035385 | MS.gene061900:CDS | 35.0% | |
| ATCTGAACATTTGTCTGAGG+GGG | + | chr7.2:12034509-12034528 | MS.gene061900:CDS | 40.0% | |
| TACCACATATGGAATAACGC+CGG | - | chr7.2:12034844-12034863 | None:intergenic | 40.0% | |
| TAGCCAAGGAAAACTCAAGT+GGG | + | chr7.2:12034547-12034566 | MS.gene061900:CDS | 40.0% | |
| TGTAGCCAAGATTCAAACTG+CGG | + | chr7.2:12035417-12035436 | MS.gene061900:CDS | 40.0% | |
| TTCACCGTCAATTTCCTATG+TGG | - | chr7.2:12035141-12035160 | None:intergenic | 40.0% | |
| TTTATCACGGCTACCTTTCA+AGG | - | chr7.2:12035109-12035128 | None:intergenic | 40.0% | |
| ! | AGAAGTGCATTACTCAGTTG+CGG | + | chr7.2:12035467-12035486 | MS.gene061900:CDS | 40.0% |
| ! | TTTTACATCACAGGCTCCTT+CGG | + | chr7.2:12035071-12035090 | MS.gene061900:intron | 40.0% |
| !! | GTGGTTCACCGTTTTTATCA+CGG | - | chr7.2:12035122-12035141 | None:intergenic | 40.0% |
| ACAAGAAACAGCCGAAATCC+AGG | + | chr7.2:12035217-12035236 | MS.gene061900:CDS | 45.0% | |
| AGCCAAGGAAAACTCAAGTG+GGG | + | chr7.2:12034548-12034567 | MS.gene061900:CDS | 45.0% | |
| GAGCTCAAATCTGCCTTGAA+AGG | + | chr7.2:12035093-12035112 | MS.gene061900:CDS | 45.0% | |
| GTAGCCAAGGAAAACTCAAG+TGG | + | chr7.2:12034546-12034565 | MS.gene061900:CDS | 45.0% | |
| TGATCTGTGCAGTAAGTCGT+GGG | + | chr7.2:12035347-12035366 | MS.gene061900:CDS | 45.0% | |
| TGATGAGCAGAATGCTCATG+AGG | - | chr7.2:12034702-12034721 | None:intergenic | 45.0% | |
| TGCGACACTAGTGTAGGTAT+AGG | - | chr7.2:12035186-12035205 | None:intergenic | 45.0% | |
| TGCTTCCGCAGTTTGAATCT+TGG | - | chr7.2:12035425-12035444 | None:intergenic | 45.0% | |
| ! | AACCCCACTTGAGTTTTCCT+TGG | - | chr7.2:12034553-12034572 | None:intergenic | 45.0% |
| AACAAGGGGAATTCAACCCG+AGG | + | chr7.2:12035276-12035295 | MS.gene061900:CDS | 50.0% | |
| AAGAAGCAGTCTCGCAACGT+TGG | + | chr7.2:12035315-12035334 | MS.gene061900:CDS | 50.0% | |
| AAGGCAGATTTGAGCTCCGA+AGG | - | chr7.2:12035090-12035109 | None:intergenic | 50.0% | |
| ACAAGGGGAATTCAACCCGA+GGG | + | chr7.2:12035277-12035296 | MS.gene061900:CDS | 50.0% | |
| ACAGTGTGCGACACTAGTGT+AGG | - | chr7.2:12035192-12035211 | None:intergenic | 50.0% | |
| AGCCGGCGTTATTCCATATG+TGG | + | chr7.2:12034839-12034858 | MS.gene061900:intron | 50.0% | |
| CGTCGCATGAGGAATTGGAA+AGG | - | chr7.2:12034628-12034647 | None:intergenic | 50.0% | |
| CTGATCTGTGCAGTAAGTCG+TGG | + | chr7.2:12035346-12035365 | MS.gene061900:CDS | 50.0% | |
| TATCTTTGCCATTGCCCCCT+CGG | - | chr7.2:12035296-12035315 | None:intergenic | 50.0% | |
| TCCTCGTCAGAAGAGCTTGA+AGG | - | chr7.2:12034654-12034673 | None:intergenic | 50.0% | |
| TGCGATGTGCAATGTAGCCA+AGG | + | chr7.2:12034533-12034552 | MS.gene061900:CDS | 50.0% | |
| AAGCAGTCTCGCAACGTTGG+TGG | + | chr7.2:12035318-12035337 | MS.gene061900:CDS | 55.0% | |
| AAGGGGAATTCAACCCGAGG+GGG | + | chr7.2:12035279-12035298 | MS.gene061900:CDS | 55.0% | |
| AATTCAACCCGAGGGGGCAA+TGG | + | chr7.2:12035285-12035304 | MS.gene061900:CDS | 55.0% | |
| AGCAGTCTCGCAACGTTGGT+GGG | + | chr7.2:12035319-12035338 | MS.gene061900:CDS | 55.0% | |
| ATCTTTGCCATTGCCCCCTC+GGG | - | chr7.2:12035295-12035314 | None:intergenic | 55.0% | |
| CAAGGGGAATTCAACCCGAG+GGG | + | chr7.2:12035278-12035297 | MS.gene061900:CDS | 55.0% | |
| CTTGAAGGCAACGTCGCATG+AGG | - | chr7.2:12034639-12034658 | None:intergenic | 55.0% | |
| GCCTTCAAGCTCTTCTGACG+AGG | + | chr7.2:12034650-12034669 | MS.gene061900:CDS | 55.0% | |
| GGCAACGTCGCATGAGGAAT+TGG | - | chr7.2:12034633-12034652 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 12034411 | 12035500 | 12034411 | ID=MS.gene061900 |
| chr7.2 | mRNA | 12034411 | 12035500 | 12034411 | ID=MS.gene061900.t1;Parent=MS.gene061900 |
| chr7.2 | exon | 12034411 | 12034749 | 12034411 | ID=MS.gene061900.t1.exon1;Parent=MS.gene061900.t1 |
| chr7.2 | CDS | 12034411 | 12034749 | 12034411 | ID=cds.MS.gene061900.t1;Parent=MS.gene061900.t1 |
| chr7.2 | exon | 12035084 | 12035500 | 12035084 | ID=MS.gene061900.t1.exon2;Parent=MS.gene061900.t1 |
| chr7.2 | CDS | 12035084 | 12035500 | 12035084 | ID=cds.MS.gene061900.t1;Parent=MS.gene061900.t1 |
| Gene Sequence |
| Protein sequence |
