Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019278.t1 | XP_003611137.1 | 96.7 | 330 | 11 | 0 | 1 | 330 | 1 | 330 | 1.30E-155 | 559.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019278.t1 | Q9LN71 | 66.7 | 288 | 88 | 3 | 50 | 330 | 65 | 351 | 1.7e-95 | 350.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019278.t1 | G7KDG0 | 96.7 | 330 | 11 | 0 | 1 | 330 | 1 | 330 | 9.2e-156 | 559.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene66767 | MS.gene019278 | PPI |
| MS.gene019278 | MS.gene62798 | PPI |
| MS.gene019278 | MS.gene072809 | PPI |
| MS.gene019278 | MS.gene034468 | PPI |
| MS.gene019278 | MS.gene43051 | PPI |
| MS.gene019278 | MS.gene015550 | PPI |
| MS.gene019278 | MS.gene019280 | PPI |
| MS.gene019278 | MS.gene050322 | PPI |
| MS.gene019278 | MS.gene72994 | PPI |
| MS.gene019278 | MS.gene007227 | PPI |
| MS.gene019278 | MS.gene84339 | PPI |
| MS.gene019278 | MS.gene35396 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019278.t1 | MTR_5g010720 | 98.503 | 334 | 5 | 0 | 1 | 334 | 1 | 334 | 0.0 | 672 |
| MS.gene019278.t1 | MTR_4g102870 | 72.000 | 325 | 77 | 6 | 13 | 334 | 5 | 318 | 2.46e-159 | 451 |
| MS.gene019278.t1 | MTR_7g076010 | 50.154 | 325 | 139 | 6 | 19 | 334 | 4 | 314 | 2.69e-89 | 273 |
| MS.gene019278.t1 | MTR_8g105330 | 49.270 | 274 | 118 | 4 | 58 | 330 | 30 | 283 | 9.37e-85 | 260 |
| MS.gene019278.t1 | MTR_1g032440 | 45.897 | 329 | 148 | 8 | 12 | 340 | 9 | 307 | 6.79e-83 | 254 |
| MS.gene019278.t1 | MTR_1g032440 | 46.395 | 319 | 141 | 8 | 12 | 330 | 9 | 297 | 1.31e-80 | 250 |
| MS.gene019278.t1 | MTR_7g088500 | 38.767 | 227 | 93 | 3 | 80 | 300 | 34 | 220 | 8.96e-47 | 161 |
| MS.gene019278.t1 | MTR_3g107995 | 29.545 | 264 | 154 | 9 | 88 | 334 | 71 | 319 | 9.55e-23 | 98.6 |
| MS.gene019278.t1 | MTR_1g011380 | 29.057 | 265 | 168 | 7 | 86 | 338 | 92 | 348 | 2.95e-21 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019278.t1 | AT4G11680 | 62.319 | 345 | 100 | 8 | 6 | 340 | 26 | 350 | 1.85e-125 | 367 |
| MS.gene019278.t1 | AT4G11680 | 63.582 | 335 | 96 | 7 | 6 | 334 | 26 | 340 | 7.26e-125 | 364 |
| MS.gene019278.t1 | AT1G12760 | 68.421 | 304 | 90 | 2 | 37 | 334 | 52 | 355 | 1.66e-123 | 362 |
| MS.gene019278.t1 | AT1G12760 | 69.014 | 284 | 82 | 2 | 57 | 334 | 1 | 284 | 2.30e-116 | 341 |
| MS.gene019278.t1 | AT1G63170 | 66.890 | 299 | 94 | 3 | 41 | 334 | 29 | 327 | 8.17e-114 | 336 |
| MS.gene019278.t1 | AT3G61180 | 58.681 | 288 | 110 | 4 | 44 | 330 | 42 | 321 | 4.16e-111 | 329 |
| MS.gene019278.t1 | AT1G68070 | 46.497 | 314 | 136 | 4 | 20 | 330 | 6 | 290 | 1.33e-88 | 270 |
| MS.gene019278.t1 | AT2G01735 | 47.266 | 256 | 116 | 3 | 53 | 307 | 44 | 281 | 1.25e-72 | 230 |
| MS.gene019278.t1 | AT2G01735 | 48.649 | 222 | 98 | 2 | 53 | 273 | 44 | 250 | 2.31e-65 | 207 |
| MS.gene019278.t1 | AT4G32600 | 28.679 | 265 | 148 | 11 | 97 | 338 | 124 | 370 | 8.18e-22 | 96.7 |
| MS.gene019278.t1 | AT4G32600 | 28.679 | 265 | 148 | 11 | 97 | 338 | 124 | 370 | 8.18e-22 | 96.7 |
| MS.gene019278.t1 | AT1G80400 | 27.542 | 236 | 133 | 7 | 86 | 298 | 106 | 326 | 9.35e-21 | 93.2 |
| MS.gene019278.t1 | AT4G26580 | 25.688 | 218 | 126 | 7 | 97 | 307 | 99 | 287 | 2.34e-11 | 64.7 |
| MS.gene019278.t1 | AT4G26580 | 25.688 | 218 | 126 | 7 | 97 | 307 | 99 | 287 | 2.34e-11 | 64.7 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCTCGTTCTGCTGTTTC+TGG | 0.113154 | 5.4:-4974259 | MS.gene019278:CDS |
| CACAGCCTCAACGGCATTTC+CGG | 0.201324 | 5.4:+4974430 | None:intergenic |
| AAGATTCTCCTCAGCTTTAC+TGG | 0.236698 | 5.4:-4972155 | MS.gene019278:intron |
| TCATTTATTTGGTGGATTAT+TGG | 0.257070 | 5.4:-4972216 | MS.gene019278:CDS |
| AGGTTATGTATAGTCTTTCT+TGG | 0.315938 | 5.4:-4971999 | MS.gene019278:CDS |
| CGGCAGTGTTGCGAAACATT+TGG | 0.324377 | 5.4:-4972259 | MS.gene019278:intron |
| GGTGGATTATTGGATTCTAC+TGG | 0.372489 | 5.4:-4972206 | MS.gene019278:CDS |
| TGGTTCCAGTTATGTTTCAT+TGG | 0.384792 | 5.4:-4974239 | MS.gene019278:CDS |
| TTGCGCCAATGAAACATAAC+TGG | 0.386200 | 5.4:+4974234 | None:intergenic |
| GCATTTCCGGAGCTTCATTC+CGG | 0.387248 | 5.4:+4974443 | None:intergenic |
| TTGTGATTCTCGATATCGTT+TGG | 0.398024 | 5.4:-4974510 | MS.gene019278:CDS |
| CCGTTGAGGCTGTGGATTGC+GGG | 0.401372 | 5.4:-4974421 | MS.gene019278:CDS |
| GTTGCAATGTGTTCTTCATA+TGG | 0.402716 | 5.4:-4974392 | MS.gene019278:CDS |
| GTTGCGCTGGCCTGTGTCAT+TGG | 0.414076 | 5.4:-4971948 | MS.gene019278:CDS |
| AGTAAAGCTGAGGAGAATCT+TGG | 0.421975 | 5.4:+4972157 | None:intergenic |
| CGCAACGTGGACATTGGAAA+AGG | 0.430138 | 5.4:+4967820 | None:intergenic |
| TCAGCAAGAACATGTTCAAT+AGG | 0.434852 | 5.4:+4967905 | None:intergenic |
| CTTCGTCATTGTGGCGGGAA+CGG | 0.437914 | 5.4:-4974479 | MS.gene019278:CDS |
| CGGAAATGCCGTTGAGGCTG+TGG | 0.443205 | 5.4:-4974429 | MS.gene019278:CDS |
| AATAAATGAAAACATGGTAT+TGG | 0.445274 | 5.4:+4972230 | None:intergenic |
| TCGTCATCGAATGCGGTAGC+AGG | 0.452616 | 5.4:-4974322 | MS.gene019278:CDS |
| AGGAATAATGACCGAGTGTC+GGG | 0.456706 | 5.4:-4967936 | MS.gene019278:CDS |
| TCTTTATGCTGTTGCAGACC+AGG | 0.456796 | 5.4:-4971886 | MS.gene019278:intron |
| GGAGGAACCGCCGCCGGGAT+TGG | 0.464726 | 5.4:+4974708 | None:intergenic |
| ATGCGGTAGCAGGTTCTGAT+AGG | 0.472844 | 5.4:-4974312 | MS.gene019278:CDS |
| GCCGCCGGGATTGGTGAGAA+AGG | 0.476623 | 5.4:+4974717 | None:intergenic |
| AAGCGGCCGTCGAACGATGC+GGG | 0.483688 | 5.4:-4974611 | MS.gene019278:CDS |
| GTAGTGGGAATTTCAGTTCA+AGG | 0.485833 | 5.4:-4974285 | MS.gene019278:CDS |
| TAGAAGAACGACAAAGCGAT+TGG | 0.486101 | 5.4:-4974549 | MS.gene019278:CDS |
| TTCGCCTTCGTCATTGTGGC+GGG | 0.487248 | 5.4:-4974484 | MS.gene019278:CDS |
| TCGGTCATTATTCCTCCAAC+AGG | 0.487971 | 5.4:+4967944 | None:intergenic |
| TTTCTCACCAATCCCGGCGG+CGG | 0.492874 | 5.4:-4974715 | MS.gene019278:CDS |
| GTAAAGCTGAGGAGAATCTT+GGG | 0.502198 | 5.4:+4972158 | None:intergenic |
| GAAGGAAGCGTGCGGCTTGG+AGG | 0.503723 | 5.4:+4974641 | None:intergenic |
| TCGGAGGAGGAACCGCCGCC+GGG | 0.504312 | 5.4:+4974703 | None:intergenic |
| ATCGGAGGAGGAACCGCCGC+CGG | 0.506560 | 5.4:+4974702 | None:intergenic |
| ACGTTGGCGACGACGGCGAC+CGG | 0.511663 | 5.4:+4974666 | None:intergenic |
| TTTGAGCCGGAATGAAGCTC+CGG | 0.515420 | 5.4:-4974449 | MS.gene019278:CDS |
| GACGAAGGAAGCGTGCGGCT+TGG | 0.516675 | 5.4:+4974638 | None:intergenic |
| ACTCCCTTTCTCACCAATCC+CGG | 0.519708 | 5.4:-4974721 | MS.gene019278:CDS |
| GGATTCTACTGGGTATCAGC+TGG | 0.519815 | 5.4:-4972195 | MS.gene019278:CDS |
| GACATTGGAAAAGGGAGAAA+AGG | 0.520186 | 5.4:+4967829 | None:intergenic |
| CGCTTCCTTCGTCAAGCAAG+CGG | 0.520375 | 5.4:-4974628 | MS.gene019278:CDS |
| AGAAGAACGACAAAGCGATT+GGG | 0.524313 | 5.4:-4974548 | MS.gene019278:CDS |
| GTGGATTATTGGATTCTACT+GGG | 0.526393 | 5.4:-4972205 | MS.gene019278:CDS |
| GTTGTTGAGGTCATCGGAGG+AGG | 0.532200 | 5.4:+4974690 | None:intergenic |
| CCGCCGGGATTGGTGAGAAA+GGG | 0.536556 | 5.4:+4974718 | None:intergenic |
| GATAACAATCAAGGTCCTGT+TGG | 0.541510 | 5.4:-4967959 | MS.gene019278:CDS |
| ACCGGAGTTGTTGAGGTCAT+CGG | 0.545010 | 5.4:+4974684 | None:intergenic |
| GCAACGTGGACATTGGAAAA+GGG | 0.546710 | 5.4:+4967821 | None:intergenic |
| AACAATCAAGGTCCTGTTGG+AGG | 0.549616 | 5.4:-4967956 | MS.gene019278:CDS |
| AAACAACGCAACGTGGACAT+TGG | 0.550325 | 5.4:+4967814 | None:intergenic |
| AAACAAACTGATAACAATCA+AGG | 0.553925 | 5.4:-4967968 | MS.gene019278:CDS |
| CCACCAAATAAATGAAAACA+TGG | 0.554733 | 5.4:+4972224 | None:intergenic |
| TTGAGTATAGGAGAAGGAGA+AGG | 0.554902 | 5.4:-4974357 | MS.gene019278:CDS |
| CGATTGGGCGTATTCGAAGC+CGG | 0.557403 | 5.4:-4974533 | MS.gene019278:CDS |
| CCCGCAATCCACAGCCTCAA+CGG | 0.557587 | 5.4:+4974421 | None:intergenic |
| TAAAGAAGTGCAATGATACA+TGG | 0.558649 | 5.4:+4971903 | None:intergenic |
| TGAACATGTTCTTGCTGAGG+AGG | 0.560231 | 5.4:-4967900 | MS.gene019278:CDS |
| GGGAATTTCAGTTCAAGGGA+AGG | 0.560360 | 5.4:-4974280 | MS.gene019278:CDS |
| AAGGCTCCCGCATCGTTCGA+CGG | 0.563222 | 5.4:+4974605 | None:intergenic |
| TAGTGGGAATTTCAGTTCAA+GGG | 0.567413 | 5.4:-4974284 | MS.gene019278:CDS |
| GGTTCTGATAGGATTGGTAG+TGG | 0.567433 | 5.4:-4974301 | MS.gene019278:CDS |
| TTCTACTGGGTATCAGCTGG+TGG | 0.568485 | 5.4:-4972192 | MS.gene019278:CDS |
| TGTTCTTGCTGAGGAGGATG+CGG | 0.568631 | 5.4:-4967894 | MS.gene019278:CDS |
| CTTCGCCTTCGTCATTGTGG+CGG | 0.571163 | 5.4:-4974485 | MS.gene019278:CDS |
| GGAGGAGACGTTGGCGACGA+CGG | 0.572322 | 5.4:+4974659 | None:intergenic |
| TCCGATGACCTCAACAACTC+CGG | 0.579073 | 5.4:-4974685 | MS.gene019278:CDS |
| ACGATATCGAGAATCACAAC+CGG | 0.580305 | 5.4:+4974514 | None:intergenic |
| AATTTGATGAAGAGAGTACT+AGG | 0.581019 | 5.4:-4974213 | MS.gene019278:intron |
| GCCGTTGAGGCTGTGGATTG+CGG | 0.586081 | 5.4:-4974422 | MS.gene019278:CDS |
| GAACTTCGCCTTCGTCATTG+TGG | 0.588422 | 5.4:-4974488 | MS.gene019278:CDS |
| GTGCGGCTTGGAGGAGACGT+TGG | 0.591058 | 5.4:+4974650 | None:intergenic |
| GACGGCGACCGGAGTTGTTG+AGG | 0.594522 | 5.4:+4974677 | None:intergenic |
| GAGGAATAATGACCGAGTGT+CGG | 0.596428 | 5.4:-4967937 | MS.gene019278:CDS |
| ACGTTCTTCGTCATCGAATG+CGG | 0.597603 | 5.4:-4974329 | MS.gene019278:CDS |
| AGGTGAATCAGCCCGACACT+CGG | 0.599908 | 5.4:+4967925 | None:intergenic |
| ATGTCTGATGAGGAACGGTT+CGG | 0.599968 | 5.4:+4974754 | None:intergenic |
| CGATGATGTCTGATGAGGAA+CGG | 0.605271 | 5.4:+4974749 | None:intergenic |
| GACGGCCGCTTGCTTGACGA+AGG | 0.611750 | 5.4:+4974623 | None:intergenic |
| GTAGCAGGTTCTGATAGGAT+TGG | 0.613195 | 5.4:-4974307 | MS.gene019278:CDS |
| CAAGCGGCCGTCGAACGATG+CGG | 0.613490 | 5.4:-4974612 | MS.gene019278:CDS |
| GTTTCGCGAACAACGACGGA+AGG | 0.617701 | 5.4:+4974586 | None:intergenic |
| GGCAGTTTCGCGAACAACGA+CGG | 0.621073 | 5.4:+4974582 | None:intergenic |
| TATTGAACATGTTCTTGCTG+AGG | 0.624382 | 5.4:-4967903 | MS.gene019278:CDS |
| AGGACAAACCAGTAAAGCTG+AGG | 0.632785 | 5.4:+4972147 | None:intergenic |
| AAGCTCCGGAAATGCCGTTG+AGG | 0.640748 | 5.4:-4974435 | MS.gene019278:CDS |
| CGTTCCCGCCACAATGACGA+AGG | 0.662267 | 5.4:+4974480 | None:intergenic |
| AACAGCGATACCAATGACAC+AGG | 0.663749 | 5.4:+4971938 | None:intergenic |
| GTTCTGATAGGATTGGTAGT+GGG | 0.672302 | 5.4:-4974300 | MS.gene019278:CDS |
| GGAGTTGTTGAGGTCATCGG+AGG | 0.672635 | 5.4:+4974687 | None:intergenic |
| TCGTTCTTCTAATTGCTCTG+CGG | 0.681822 | 5.4:+4974561 | None:intergenic |
| TGAATCGATGATGTCTGATG+AGG | 0.686853 | 5.4:+4974744 | None:intergenic |
| TGATTAGAAACAACGCAACG+TGG | 0.704031 | 5.4:+4967807 | None:intergenic |
| CCCTTTCTCACCAATCCCGG+CGG | 0.736966 | 5.4:-4974718 | MS.gene019278:CDS |
| TGCTTGACGAAGGAAGCGTG+CGG | 0.756061 | 5.4:+4974633 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 4967821 | 4974785 | 4967821 | ID=MS.gene019278 |
| chr5.4 | mRNA | 4967821 | 4974785 | 4967821 | ID=MS.gene019278.t1;Parent=MS.gene019278 |
| chr5.4 | exon | 4974214 | 4974785 | 4974214 | ID=MS.gene019278.t1.exon1;Parent=MS.gene019278.t1 |
| chr5.4 | CDS | 4974214 | 4974785 | 4974214 | ID=cds.MS.gene019278.t1;Parent=MS.gene019278.t1 |
| chr5.4 | exon | 4972156 | 4972275 | 4972156 | ID=MS.gene019278.t1.exon2;Parent=MS.gene019278.t1 |
| chr5.4 | CDS | 4972156 | 4972275 | 4972156 | ID=cds.MS.gene019278.t1;Parent=MS.gene019278.t1 |
| chr5.4 | exon | 4971887 | 4972019 | 4971887 | ID=MS.gene019278.t1.exon3;Parent=MS.gene019278.t1 |
| chr5.4 | CDS | 4971887 | 4972019 | 4971887 | ID=cds.MS.gene019278.t1;Parent=MS.gene019278.t1 |
| chr5.4 | exon | 4967821 | 4968059 | 4967821 | ID=MS.gene019278.t1.exon4;Parent=MS.gene019278.t1 |
| chr5.4 | CDS | 4967821 | 4968059 | 4967821 | ID=cds.MS.gene019278.t1;Parent=MS.gene019278.t1 |
| Gene Sequence |
| Protein sequence |
